Rroxscaffold_4G00282640

alpha-L-fucosidase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0003070
Physical Location & Seq
Forward (+)
4738491 .. 4739605
1115 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00282640.1

Sequence Viewer

Length: 828 bp
ATGGTGAGGGAGTTACAGAGCTCCATTGACATATTCTCGGATGCTGATCCTGACGTTAGATGGGTCGGCGATGAGCAAGGCTTCGCCGGAGGCACAAGCTGGTCCACCATTAATCAGAGTTCATTATCAATAGGGAATGCAAGCATTGAAGGTTATCTCAACACTGATGATCCACAAGGAACAGACTGGTTGCCGCCAGAATGTGACGTTTCGATAAGAAATGGATGGTTTTGGCACAAATCAGAGTCGCCTAAGAAGCTAAGCAAGCTACTTGAAATTTACTACAACTCTGTAGGAAGAAATTGTGTGCTGTTACTCAATGTGCCTCCTAATTCGACCGGACTTATATCCGAAGCAGATGTGCAAAGACTCAGAGAATTGAGAGGTGCAATTGACACAATATTTTCTAGCAATTTAGCTGATAAATGCTTCATCAAACTGAGTAGCCAACTAGAAGGCAAAGGAGGAGGTTTTGGACCTGAAAATGTGCTAGACAGTGACCATTTGTGGACATATTGGGCTCCAAAGGATGGTGACAAAGAGGAGCATTGGATTGAATTTAGAGGCAGAGATAAAGGATTCAGGTTCCATGTGATTAGGATTCAAGAGGCAATCGGGCTTGGCCAGAGAATCAAAAGGCATGAAATTTATGTGGATGGTAAGAGGGTTGCAAATGGAACCACAGTGGGGTACAAGAGGCTTCACAGACTTGAAGGGGGAGTAGTCCATGGCCAAATTGTCAGAATTATAATTAAAGACTCAAAAGGGCTGCTTTTGATTTCTTCCATTGGTCTACATTTTGATCCCTATTGGGACCAAAGTGGCTAG

Protein Analysis

275

Amino Acids

30.87

Weight (kDa)

5.92

Isoelectric Point (pI)

38.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Alpha_L_fucos PF01120 67 - 127 6.4e-06 Alpha-L-fucosidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 749
AccB7I CCANNNNNTGG 1 cut(s) 530
AccI GTMKAC 1 cut(s) 793
AciI CCGC 1 cut(s) 194
AclWI GGATC 3 cut(s) 41, 164, 797
AcoI YGGCCR 2 cut(s) 622, 730
AcsI RAATTY 3 cut(s) 276, 557, 645
AfaI GTAC 1 cut(s) 692
AfiI CCNNNNNNNGG 2 cut(s) 530, 687
AgsI TTSAA 5 cut(s) 149, 275, 557, 605, 713
AluBI AGCT 5 cut(s) 21, 99, 259, 268, 419
AluI AGCT 5 cut(s) 21, 99, 259, 268, 419
Alw21I GWGCWC 1 cut(s) 23
AlwI GGATC 3 cut(s) 41, 164, 797
AoxI GGCC 2 cut(s) 622, 730
ApeKI GCWGC 1 cut(s) 769
ApoI RAATTY 3 cut(s) 276, 557, 645
AseI ATTAAT 1 cut(s) 111
AspS9I GGNCC 3 cut(s) 102, 476, 814
AsuHPI GGTGA 2 cut(s) 16, 545
AvaII GGWCC 3 cut(s) 102, 476, 814
BalI TGGCCA 2 cut(s) 624, 732
BanII GRGCYC 2 cut(s) 23, 523
Bbv12I GWGCWC 1 cut(s) 23
BbvI GCAGC 1 cut(s) 756
BccI CCATC 4 cut(s) 54, 219, 524, 650
BfaI CTAG 4 cut(s) 408, 452, 491, 826
BfmI CTRYAG 1 cut(s) 291
BisI GCNGC 2 cut(s) 194, 770
BlpI GCTNAGC 1 cut(s) 260
BlsI GCNGC 2 cut(s) 195, 771
Bme18I GGWCC 3 cut(s) 102, 476, 814
BmgT120I GGNCC 3 cut(s) 102, 476, 814
BmiI GGNNCC 4 cut(s) 522, 587, 679, 815
BmsI GCATC 1 cut(s) 31
Bpu1102I GCTNAGC 1 cut(s) 260
BsaBI GATNNNNATC 1 cut(s) 45
BsaJI CCNNGG 1 cut(s) 727
BsaWI WCCGGW 1 cut(s) 338
Bsc4I CCNNNNNNNGG 2 cut(s) 530, 687
Bse1I ACTGG 1 cut(s) 191
Bse8I GATNNNNATC 1 cut(s) 45
BseDI CCNNGG 1 cut(s) 727
BseGI GGATG 4 cut(s) 46, 230, 535, 661
BseJI GATNNNNATC 1 cut(s) 45
BseLI CCNNNNNNNGG 2 cut(s) 530, 687
BseMII CTCAG 2 cut(s) 385, 431
BseNI ACTGG 1 cut(s) 191
BseRI GAGGAG 2 cut(s) 480, 557
BseXI GCAGC 1 cut(s) 756
Bsh1285I CGRYCG 1 cut(s) 339
BshFI GGCC 2 cut(s) 624, 732
BsiEI CGRYCG 1 cut(s) 339
BsiHKAI GWGCWC 1 cut(s) 23
BsiSI CCGG 2 cut(s) 87, 339
BslI CCNNNNNNNGG 2 cut(s) 530, 687
BsmI GAATGC 1 cut(s) 142
BsnI GGCC 2 cut(s) 624, 732
Bsp1286I GDGCHC 2 cut(s) 23, 523
Bsp143I GATC 3 cut(s) 46, 169, 802
Bsp1720I GCTNAGC 1 cut(s) 260
Bsp19I CCATGG 1 cut(s) 727
BspACI CCGC 1 cut(s) 194
BspANI GGCC 2 cut(s) 624, 732
BspCNI CTCAG 2 cut(s) 384, 432
BspLI GGNNCC 4 cut(s) 522, 587, 679, 815
BspPI GGATC 3 cut(s) 41, 164, 797
BsrI ACTGG 1 cut(s) 191
BssECI CCNNGG 1 cut(s) 727
BssMI GATC 3 cut(s) 46, 169, 802
BssT1I CCWWGG 1 cut(s) 727
Bst4CI ACNGT 2 cut(s) 497, 685
BstC8I GCNNGC 2 cut(s) 142, 266
BstDEI CTNAG 4 cut(s) 252, 260, 371, 440
BstDSI CCRYGG 1 cut(s) 727
BstF5I GGATG 4 cut(s) 46, 230, 535, 661
BstKTI GATC 3 cut(s) 49, 172, 805
BstMBI GATC 3 cut(s) 46, 169, 802
BstMCI CGRYCG 1 cut(s) 339
BstMWI GCNNNNNNNGC 2 cut(s) 256, 265
BstSFI CTRYAG 1 cut(s) 291
BstV1I GCAGC 1 cut(s) 756
BsuRI GGCC 2 cut(s) 624, 732
BtgI CCRYGG 1 cut(s) 727
BtgZI GCGATG 1 cut(s) 84
BtsCI GGATG 4 cut(s) 46, 230, 535, 661
BtsIMutI CAGTG 3 cut(s) 162, 502, 690
Cac8I GCNNGC 2 cut(s) 142, 266
Cfr13I GGNCC 3 cut(s) 102, 476, 814
Csp6I GTAC 1 cut(s) 691
CviAII CATG 3 cut(s) 590, 641, 728
CviQI GTAC 1 cut(s) 691
DdeI CTNAG 4 cut(s) 252, 260, 371, 440
DpnI GATC 3 cut(s) 48, 171, 804
DpnII GATC 3 cut(s) 46, 169, 802
EaeI YGGCCR 2 cut(s) 622, 730
Ecl136II GAGCTC 1 cut(s) 21
Eco130I CCWWGG 1 cut(s) 727
Eco24I GRGCYC 2 cut(s) 23, 523
Eco47I GGWCC 3 cut(s) 102, 476, 814
Eco53kI GAGCTC 1 cut(s) 21
EcoICRI GAGCTC 1 cut(s) 21
EcoT14I CCWWGG 1 cut(s) 727
EcoT38I GRGCYC 2 cut(s) 23, 523
ErhI CCWWGG 1 cut(s) 727
FaeI CATG 3 cut(s) 593, 644, 731
FaiI YATR 8 cut(s) 32, 347, 514, 591, 642, 651, 729, 749
FalI AAGNNNNNCTT 2 cut(s) 756, 788
FatI CATG 3 cut(s) 589, 640, 727
FblI GTMKAC 1 cut(s) 793
Fnu4HI GCNGC 2 cut(s) 194, 770
FokI GGATG 4 cut(s) 53, 237, 542, 668
FriOI GRGCYC 2 cut(s) 23, 523
Fsp4HI GCNGC 2 cut(s) 194, 770
FspBI CTAG 4 cut(s) 408, 452, 491, 826
GluI GCNGC 2 cut(s) 194, 770
HaeIII GGCC 2 cut(s) 624, 732
HapII CCGG 2 cut(s) 87, 339
Hin1II CATG 3 cut(s) 593, 644, 731
HinfI GANTC 6 cut(s) 245, 369, 579, 601, 630, 758
HpaII CCGG 2 cut(s) 87, 339
HphI GGTGA 2 cut(s) 16, 545
Hpy166II GTNNAC 3 cut(s) 105, 510, 794
Hpy188I TCNGA 6 cut(s) 40, 117, 244, 352, 374, 743
Hpy188III TCNNGA 2 cut(s) 50, 605
Hpy8I GTNNAC 3 cut(s) 105, 510, 794
HpyAV CCTTC 3 cut(s) 143, 449, 707
HpyCH4III ACNGT 2 cut(s) 497, 685
HpyCH4IV ACGT 2 cut(s) 54, 207
HpyCH4V TGCA 4 cut(s) 140, 364, 389, 671
HpyF10VI GCNNNNNNNGC 2 cut(s) 256, 265
HpyF3I CTNAG 4 cut(s) 252, 260, 371, 440
HpySE526I ACGT 2 cut(s) 54, 207
Hsp92II CATG 3 cut(s) 593, 644, 731
Kzo9I GATC 3 cut(s) 46, 169, 802
LmnI GCTCC 3 cut(s) 26, 526, 544
LpnPI CCDG 9 cut(s) 63, 85, 100, 172, 210, 352, 492, 568, 638
Lsp1109I GCAGC 1 cut(s) 756
LweI GCATC 1 cut(s) 31
MaeI CTAG 4 cut(s) 408, 452, 491, 826
MaeII ACGT 2 cut(s) 54, 207
MaeIII GTNAC 5 cut(s) 12, 203, 312, 497, 533
MalI GATC 3 cut(s) 48, 171, 804
MboI GATC 3 cut(s) 46, 169, 802
MboII GAAGA 2 cut(s) 309, 774
MfeI CAATTG 1 cut(s) 390
MhlI GDGCHC 2 cut(s) 23, 523
MlsI TGGCCA 2 cut(s) 624, 732
MluNI TGGCCA 2 cut(s) 624, 732
MlyI GAGTC 3 cut(s) 254, 363, 752
Mox20I TGGCCA 2 cut(s) 624, 732
MscI TGGCCA 2 cut(s) 624, 732
MseI TTAA 2 cut(s) 111, 753
Msp20I TGGCCA 2 cut(s) 624, 732
MspI CCGG 2 cut(s) 87, 339
MunI CAATTG 1 cut(s) 390
Mva1269I GAATGC 1 cut(s) 142
MwoI GCNNNNNNNGC 2 cut(s) 256, 265
NcoI CCATGG 1 cut(s) 727
NdeII GATC 3 cut(s) 46, 169, 802
NlaIII CATG 3 cut(s) 593, 644, 731
NlaIV GGNNCC 4 cut(s) 522, 587, 679, 815
NmuCI GTSAC 3 cut(s) 203, 497, 533
PctI GAATGC 1 cut(s) 142
PfeI GAWTC 3 cut(s) 579, 601, 630
PflMI CCANNNNNTGG 1 cut(s) 530
PkrI GCNGC 2 cut(s) 195, 771
PleI GAGTC 3 cut(s) 253, 363, 752
PpsI GAGTC 3 cut(s) 253, 363, 752
PshBI ATTAAT 1 cut(s) 111
PsiI TTATAA 1 cut(s) 749
Psp124BI GAGCTC 1 cut(s) 23
PspN4I GGNNCC 4 cut(s) 522, 587, 679, 815
PspPI GGNCC 3 cut(s) 102, 476, 814
RsaI GTAC 1 cut(s) 692
RsaNI GTAC 1 cut(s) 691
SacI GAGCTC 1 cut(s) 23
SaqAI TTAA 2 cut(s) 111, 753
SatI GCNGC 2 cut(s) 194, 770
Sau3AI GATC 3 cut(s) 46, 169, 802
Sau96I GGNCC 3 cut(s) 102, 476, 814
SchI GAGTC 3 cut(s) 254, 363, 752
SduI GDGCHC 2 cut(s) 23, 523
SfaNI GCATC 1 cut(s) 31
SfcI CTRYAG 1 cut(s) 291
SinI GGWCC 3 cut(s) 102, 476, 814
SsiI CCGC 1 cut(s) 194
SspI AATATT 1 cut(s) 402
SspMI CTAG 4 cut(s) 408, 452, 491, 826
SstI GAGCTC 1 cut(s) 23
StyI CCWWGG 1 cut(s) 727
TaaI ACNGT 2 cut(s) 497, 685
TaiI ACGT 2 cut(s) 57, 210
TaqI TCGA 2 cut(s) 212, 335
TauI GCSGC 1 cut(s) 196
TfiI GAWTC 3 cut(s) 579, 601, 630
Tru1I TTAA 2 cut(s) 111, 753
Tru9I TTAA 2 cut(s) 111, 753
TscAI CASTG 3 cut(s) 169, 502, 690
TseFI GTSAC 3 cut(s) 203, 497, 533
TseI GCWGC 1 cut(s) 769
Tsp45I GTSAC 3 cut(s) 203, 497, 533
TspDTI ATGAA 3 cut(s) 111, 421, 657
TspRI CASTG 3 cut(s) 169, 502, 690
Van91I CCANNNNNTGG 1 cut(s) 530
VpaK11BI GGWCC 3 cut(s) 102, 476, 814
VspI ATTAAT 1 cut(s) 111
XapI RAATTY 3 cut(s) 276, 557, 645
XmiI GTMKAC 1 cut(s) 793
XspI CTAG 4 cut(s) 408, 452, 491, 826
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.