Rh1DG397300

alpha-L-fucosidase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0003070
Physical Location & Seq
Reverse (-)
61654132 .. 61660441
6310 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG397300.1

Sequence Viewer

Length: 1464 bp
ATGGGTAAACAATGGTGCTCATTGTTCATAATCACGTTCTTTGAACTATGCACATTTACCTTCTCTAGGCTTGAGCAAGTAACAACACCACCCTTGCCAGTTCTACCACTTCCTACATATCAACAACTGAAATGGCAACAAAGGGAGATAATAATGTTCCTCCATTTTGGAGTGAACACATTCACTGGCCTTGAGCGTGGCACCGGGCATGAAAACCCAGCCATATTCAACCCAGTTGCGCTTGATGCGAGCCAATGGGTGAATGTAGCAGAAGATGCAGGCATATCACTAATGATACTTACTGCGAAGCACCATGATGGGTTTTGCTTGTGGCCTTCTAAGTACACTGACCATTCTGTGGAAAGAAGTCCTTGGAAAAATGGCCATGGCGATGTTGTTCAGGAGTTGGTTAATGCGGCTAAGCCCCGTGGGATTGACGTTGGGCTGTATCTCTCACCCTGGGACAGACATGATCGCAGATATGGGCTGGAGAAGCCATATAACGAATACTACTTGGCTCAGTTACAAGAGCTACTCAGTAGATATGGGAGTTTAAGAGAAATTTGGTTTGATGGATATAAAGGTGAACATGCGCCGAACATGTCCTATAATTTTACAGATTGGTTCTCTATGGTGAAGGAGTTGCAGAGTTCCATTAACATATTTTCTGATGCTGGTCCTGATGTCAGATGGGTTGGAAATGAGGCTGGGTTTGCGGGGAAAACATGCTGGTCCACCATCAATCGGACCTCGTTATCAATTGGGAATGCAGGCATTGAGGATTATCTCAACAAAGGTGATCCAAAAGGGACAGATTGGTTGCCAGCAGAGTGCGATGTCTCAATCAGAAAAGGATGGTTTTGGCATAGTTCAGAGTCACCTAAGAAGCTAAGCCAGCTACTCCAAATTTACTATAACTCAGTAGGACGAAATGCTGTGTTACTACTCAACGTGCCTCCTAATTCGACAGGGCTCATATCCGAAGTAGATGTCCAAAGACTCAGAGAATTCAGAATTGCAATCGACACCATATTTTCGACCAATGTAGCAGAGAAATGCTCCATCAAGGTGAGTAGCCAGAGAGGGGGTACAGGAGGAGGTTTTGGACCTGAGAATGTGCTAGACAGTGACCATTTGTGGACATATTGGGCTCCAAGGGATGGTGACAAAGAAGACCACTGGATTGAATTCAGAGCAGATGAAGGGATGAGGTTCAATGTGATCAGGATTCAAGAGGCGATTGGGCTAGGTCAGAGGATCAAACGGCACGAAATTTGTGTCGATGGTAAAATAGTAGCTAATGGAACCACAGTGGGTCACAAGAGACTTCACAGACTTGAAGAGGGAGTAGTCCATGGCCAAACAGTAAGAATTAGAATTAAAGAATCAAGAGGAGGGCTTCCTTTAATTTCATCCATTGGCCTGCATTTTGATCCCTATTGGCACCCAGATAAAGGTGTGTGA

Protein Analysis

487

Amino Acids

55.28

Weight (kDa)

6.39

Isoelectric Point (pI)

38.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Alpha_L_fucos PF01120 74 - 336 8.3e-35 Alpha-L-fucosidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 200, 1443
AccB7I CCANNNNNTGG 2 cut(s) 358, 1160
AciI CCGC 2 cut(s) 416, 716
AclWI GGATC 3 cut(s) 794, 1265, 1427
AcoI YGGCCR 2 cut(s) 382, 1357
AcsI RAATTY 5 cut(s) 561, 906, 1007, 1187, 1272
AfaI GTAC 2 cut(s) 344, 1090
AfiI CCNNNNNNNGG 6 cut(s) 66, 358, 744, 1084, 1160, 1454
AflIII ACRYGT 1 cut(s) 600
AgsI TTSAA 6 cut(s) 44, 229, 1187, 1216, 1232, 1340
AjnI CCWGG 1 cut(s) 458
AluBI AGCT 4 cut(s) 532, 889, 898, 1298
AluI AGCT 4 cut(s) 532, 889, 898, 1298
Alw21I GWGCWC 1 cut(s) 20
Alw26I GTCTC 2 cut(s) 844, 1318
AlwI GGATC 3 cut(s) 794, 1265, 1427
AoxI GGCC 5 cut(s) 187, 332, 382, 1357, 1420
ApoI RAATTY 5 cut(s) 561, 906, 1007, 1187, 1272
ArsI GACNNNNNNTTYG 4 cut(s) 975, 1007, 1263, 1295
Asp700I GAANNNNTTC 1 cut(s) 179
AspLEI GCGC 2 cut(s) 241, 595
AspS9I GGNCC 4 cut(s) 677, 732, 747, 1106
AsuC2I CCSGG 1 cut(s) 205
AsuHPI GGTGA 8 cut(s) 271, 447, 596, 646, 809, 870, 1081, 1175
AvaII GGWCC 4 cut(s) 677, 732, 747, 1106
BalI TGGCCA 2 cut(s) 384, 1359
BanI GGYRCC 2 cut(s) 200, 1443
BanII GRGCYC 2 cut(s) 975, 1153
BbsI GAAGAC 1 cut(s) 1179
Bbv12I GWGCWC 1 cut(s) 20
BccI CCATC 8 cut(s) 311, 566, 684, 746, 849, 1070, 1154, 1277
BceAI ACGGC 1 cut(s) 1280
BciT130I CCWGG 1 cut(s) 460
BclI TGATCA 1 cut(s) 1221
BcnI CCSGG 1 cut(s) 205
BcoDI GTCTC 2 cut(s) 844, 1318
BfaI CTAG 3 cut(s) 66, 1121, 1247
BisI GCNGC 1 cut(s) 417
BlpI GCTNAGC 2 cut(s) 420, 890
BlsI GCNGC 1 cut(s) 418
Bme1390I CCNGG 2 cut(s) 205, 460
Bme18I GGWCC 4 cut(s) 677, 732, 747, 1106
BmgT120I GGNCC 4 cut(s) 677, 732, 747, 1106
BmiI GGNNCC 4 cut(s) 202, 1152, 1306, 1445
BmrFI CCNGG 2 cut(s) 205, 460
BmrI ACTGGG 1 cut(s) 227
BmsI GCATC 3 cut(s) 235, 265, 661
BmuI ACTGGG 1 cut(s) 227
BpiI GAAGAC 1 cut(s) 1179
BplI GAGNNNNNCTC 2 cut(s) 1043, 1075
BpmI CTGGAG 1 cut(s) 509
Bpu1102I GCTNAGC 2 cut(s) 420, 890
BpuEI CTTGAG 2 cut(s) 92, 212
BpuMI CCSGG 1 cut(s) 205
BsaJI CCNNGG 7 cut(s) 371, 385, 427, 458, 459, 1154, 1354
Bsc4I CCNNNNNNNGG 6 cut(s) 66, 358, 744, 1084, 1160, 1454
Bse1I ACTGG 4 cut(s) 98, 190, 233, 1184
BseBI CCWGG 1 cut(s) 460
BseDI CCNNGG 7 cut(s) 371, 385, 427, 458, 459, 1154, 1354
BseGI GGATG 4 cut(s) 860, 1165, 1212, 1412
BseLI CCNNNNNNNGG 6 cut(s) 66, 358, 744, 1084, 1160, 1454
BseMII CTCAG 5 cut(s) 533, 550, 933, 1015, 1101
BseNI ACTGG 4 cut(s) 98, 190, 233, 1184
BseRI GAGGAG 2 cut(s) 1110, 1407
BseYI CCCAGC 2 cut(s) 217, 707
BshFI GGCC 5 cut(s) 189, 334, 384, 1359, 1422
BshNI GGYRCC 2 cut(s) 200, 1443
BsiHKAI GWGCWC 1 cut(s) 20
BsiSI CCGG 1 cut(s) 204
BslFI GGGAC 2 cut(s) 476, 823
BslI CCNNNNNNNGG 6 cut(s) 66, 358, 744, 1084, 1160, 1454
BsmAI GTCTC 2 cut(s) 844, 1318
BsmFI GGGAC 2 cut(s) 476, 823
BsmI GAATGC 1 cut(s) 772
BsnI GGCC 5 cut(s) 189, 334, 384, 1359, 1422
Bsp1286I GDGCHC 3 cut(s) 20, 975, 1153
Bsp143I GATC 5 cut(s) 472, 799, 1221, 1257, 1432
Bsp1720I GCTNAGC 2 cut(s) 420, 890
Bsp19I CCATGG 2 cut(s) 385, 1354
BspACI CCGC 2 cut(s) 416, 716
BspANI GGCC 5 cut(s) 189, 334, 384, 1359, 1422
BspCNI CTCAG 5 cut(s) 532, 549, 932, 1014, 1102
BspLI GGNNCC 4 cut(s) 202, 1152, 1306, 1445
BspPI GGATC 3 cut(s) 794, 1265, 1427
BspT107I GGYRCC 2 cut(s) 200, 1443
BsrI ACTGG 4 cut(s) 98, 190, 233, 1184
BssECI CCNNGG 7 cut(s) 371, 385, 427, 458, 459, 1154, 1354
BssMI GATC 5 cut(s) 472, 799, 1221, 1257, 1432
BssT1I CCWWGG 4 cut(s) 371, 385, 1154, 1354
Bst2UI CCWGG 1 cut(s) 460
Bst4CI ACNGT 3 cut(s) 1127, 1312, 1366
Bst6I CTCTTC 1 cut(s) 1335
BstAPI GCANNNNNTGC 1 cut(s) 275
BstC8I GCNNGC 6 cut(s) 250, 280, 772, 825, 896, 1424
BstDEI CTNAG 9 cut(s) 339, 420, 519, 536, 882, 890, 919, 1001, 1110
BstDSI CCRYGG 3 cut(s) 385, 427, 1354
BstENI CCTNNNNNAGG 1 cut(s) 64
BstF5I GGATG 4 cut(s) 860, 1165, 1212, 1412
BstHHI GCGC 2 cut(s) 241, 595
BstKTI GATC 5 cut(s) 475, 802, 1224, 1260, 1435
BstMAI GTCTC 2 cut(s) 844, 1318
BstMBI GATC 5 cut(s) 472, 799, 1221, 1257, 1432
BstMWI GCNNNNNNNGC 5 cut(s) 245, 275, 493, 713, 895
BstNI CCWGG 1 cut(s) 460
BstNSI RCATGY 3 cut(s) 593, 604, 729
BstSCI CCNGG 2 cut(s) 203, 458
BstV2I GAAGAC 1 cut(s) 1179
BsuRI GGCC 5 cut(s) 189, 334, 384, 1359, 1422
BtgI CCRYGG 3 cut(s) 385, 427, 1354
BtgZI GCGATG 2 cut(s) 405, 849
BtsCI GGATG 4 cut(s) 860, 1165, 1212, 1412
BtsIMutI CAGTG 5 cut(s) 183, 345, 1132, 1177, 1317
Cac8I GCNNGC 6 cut(s) 250, 280, 772, 825, 896, 1424
CfoI GCGC 2 cut(s) 241, 595
Cfr13I GGNCC 4 cut(s) 677, 732, 747, 1106
Csp6I GTAC 2 cut(s) 343, 1089
CviAII CATG 8 cut(s) 209, 314, 386, 470, 590, 601, 726, 1355
CviQI GTAC 2 cut(s) 343, 1089
DdeI CTNAG 9 cut(s) 339, 420, 519, 536, 882, 890, 919, 1001, 1110
DpnI GATC 5 cut(s) 474, 801, 1223, 1259, 1434
DpnII GATC 5 cut(s) 472, 799, 1221, 1257, 1432
EaeI YGGCCR 2 cut(s) 382, 1357
Eam1104I CTCTTC 1 cut(s) 1335
EarI CTCTTC 1 cut(s) 1335
Eco130I CCWWGG 4 cut(s) 371, 385, 1154, 1354
Eco24I GRGCYC 2 cut(s) 975, 1153
Eco47I GGWCC 4 cut(s) 677, 732, 747, 1106
EcoNI CCTNNNNNAGG 1 cut(s) 64
EcoRI GAATTC 2 cut(s) 1007, 1187
EcoRII CCWGG 1 cut(s) 458
EcoT14I CCWWGG 4 cut(s) 371, 385, 1154, 1354
EcoT38I GRGCYC 2 cut(s) 975, 1153
ErhI CCWWGG 4 cut(s) 371, 385, 1154, 1354
FaeI CATG 8 cut(s) 212, 317, 389, 473, 593, 604, 729, 1358
FalI AAGNNNNNCTT 2 cut(s) 355, 387
FaqI GGGAC 2 cut(s) 476, 823
FatI CATG 8 cut(s) 208, 313, 385, 469, 589, 600, 725, 1354
FauI CCCGC 1 cut(s) 709
FbaI TGATCA 1 cut(s) 1221
Fnu4HI GCNGC 1 cut(s) 417
FokI GGATG 4 cut(s) 867, 1172, 1219, 1399
FriOI GRGCYC 2 cut(s) 975, 1153
Fsp4HI GCNGC 1 cut(s) 417
FspBI CTAG 3 cut(s) 66, 1121, 1247
GlaI GCGC 2 cut(s) 240, 594
GluI GCNGC 1 cut(s) 417
GsaI CCCAGC 2 cut(s) 221, 711
GsuI CTGGAG 1 cut(s) 509
HaeIII GGCC 5 cut(s) 189, 334, 384, 1359, 1422
HapII CCGG 1 cut(s) 204
HhaI GCGC 2 cut(s) 241, 595
Hin1II CATG 8 cut(s) 212, 317, 389, 473, 593, 604, 729, 1358
Hin6I GCGC 2 cut(s) 239, 593
HinP1I GCGC 2 cut(s) 239, 593
HinfI GANTC 4 cut(s) 875, 999, 1228, 1385
HpaII CCGG 1 cut(s) 204
HphI GGTGA 8 cut(s) 271, 447, 596, 646, 809, 870, 1081, 1175
Hpy166II GTNNAC 6 cut(s) 8, 175, 345, 587, 735, 1140
Hpy188III TCNNGA 5 cut(s) 401, 680, 1225, 1232, 1389
Hpy8I GTNNAC 6 cut(s) 8, 175, 345, 587, 735, 1140
HpyAV CCTTC 4 cut(s) 70, 345, 631, 1196
HpyCH4III ACNGT 3 cut(s) 1127, 1312, 1366
HpyCH4IV ACGT 3 cut(s) 35, 438, 951
HpyCH4V TGCA 6 cut(s) 51, 278, 646, 770, 1019, 1426
HpyF10VI GCNNNNNNNGC 5 cut(s) 245, 275, 493, 713, 895
HpyF3I CTNAG 9 cut(s) 339, 420, 519, 536, 882, 890, 919, 1001, 1110
HpySE526I ACGT 3 cut(s) 35, 438, 951
Hsp92II CATG 8 cut(s) 212, 317, 389, 473, 593, 604, 729, 1358
HspAI GCGC 2 cut(s) 239, 593
Ksp22I TGATCA 1 cut(s) 1221
Kzo9I GATC 5 cut(s) 472, 799, 1221, 1257, 1432
LmnI GCTCC 2 cut(s) 1064, 1156
LweI GCATC 3 cut(s) 235, 265, 661
MaeI CTAG 3 cut(s) 66, 1121, 1247
MaeII ACGT 3 cut(s) 35, 438, 951
MaeIII GTNAC 7 cut(s) 79, 522, 876, 939, 1127, 1163, 1316
MalI GATC 5 cut(s) 474, 801, 1223, 1259, 1434
MboI GATC 5 cut(s) 472, 799, 1221, 1257, 1432
MboII GAAGA 3 cut(s) 284, 1184, 1352
MfeI CAATTG 1 cut(s) 759
MhlI GDGCHC 3 cut(s) 20, 975, 1153
MlsI TGGCCA 2 cut(s) 384, 1359
MluNI TGGCCA 2 cut(s) 384, 1359
MlyI GAGTC 2 cut(s) 884, 993
MmeI TCCRAC 1 cut(s) 676
Mox20I TGGCCA 2 cut(s) 384, 1359
MroXI GAANNNNTTC 1 cut(s) 179
MscI TGGCCA 2 cut(s) 384, 1359
MseI TTAA 5 cut(s) 411, 554, 657, 1380, 1406
MslI CAYNNNNRTG 3 cut(s) 315, 390, 1067
Msp20I TGGCCA 2 cut(s) 384, 1359
MspI CCGG 1 cut(s) 204
MspR9I CCNGG 2 cut(s) 205, 460
MunI CAATTG 1 cut(s) 759
Mva1269I GAATGC 1 cut(s) 772
MvaI CCWGG 1 cut(s) 460
MwoI GCNNNNNNNGC 5 cut(s) 245, 275, 493, 713, 895
NciI CCSGG 1 cut(s) 205
NcoI CCATGG 2 cut(s) 385, 1354
NdeII GATC 5 cut(s) 472, 799, 1221, 1257, 1432
NlaIII CATG 8 cut(s) 212, 317, 389, 473, 593, 604, 729, 1358
NlaIV GGNNCC 4 cut(s) 202, 1152, 1306, 1445
NmuCI GTSAC 4 cut(s) 876, 1127, 1163, 1316
NspI RCATGY 3 cut(s) 593, 604, 729
PasI CCCWGGG 1 cut(s) 459
PciI ACATGT 1 cut(s) 600
PctI GAATGC 1 cut(s) 772
PdmI GAANNNNTTC 1 cut(s) 179
PfeI GAWTC 2 cut(s) 1228, 1385
PflMI CCANNNNNTGG 2 cut(s) 358, 1160
PkrI GCNGC 1 cut(s) 418
PleI GAGTC 2 cut(s) 883, 993
PpsI GAGTC 2 cut(s) 883, 993
PscI ACATGT 1 cut(s) 600
Psp6I CCWGG 1 cut(s) 458
PspFI CCCAGC 2 cut(s) 217, 707
PspGI CCWGG 1 cut(s) 458
PspN4I GGNNCC 4 cut(s) 202, 1152, 1306, 1445
PspPI GGNCC 4 cut(s) 677, 732, 747, 1106
RsaI GTAC 2 cut(s) 344, 1090
RsaNI GTAC 2 cut(s) 343, 1089
RseI CAYNNNNRTG 3 cut(s) 315, 390, 1067
SaqAI TTAA 5 cut(s) 411, 554, 657, 1380, 1406
SatI GCNGC 1 cut(s) 417
Sau3AI GATC 5 cut(s) 472, 799, 1221, 1257, 1432
Sau96I GGNCC 4 cut(s) 677, 732, 747, 1106
SchI GAGTC 2 cut(s) 884, 993
ScrFI CCNGG 2 cut(s) 205, 460
SduI GDGCHC 3 cut(s) 20, 975, 1153
SfaNI GCATC 3 cut(s) 235, 265, 661
SinI GGWCC 4 cut(s) 677, 732, 747, 1106
SmiMI CAYNNNNRTG 3 cut(s) 315, 390, 1067
SmlI CTYRAG 2 cut(s) 71, 191
SmoI CTYRAG 2 cut(s) 71, 191
SsiI CCGC 2 cut(s) 416, 716
SspMI CTAG 3 cut(s) 66, 1121, 1247
StyD4I CCNGG 2 cut(s) 203, 458
StyI CCWWGG 4 cut(s) 371, 385, 1154, 1354
TaaI ACNGT 3 cut(s) 1127, 1312, 1366
TaiI ACGT 3 cut(s) 38, 441, 954
TaqI TCGA 4 cut(s) 965, 1023, 1037, 1281
TatI WGTACW 1 cut(s) 342
TauI GCSGC 1 cut(s) 419
TfiI GAWTC 2 cut(s) 1228, 1385
Tru1I TTAA 5 cut(s) 411, 554, 657, 1380, 1406
Tru9I TTAA 5 cut(s) 411, 554, 657, 1380, 1406
TscAI CASTG 5 cut(s) 190, 352, 1132, 1184, 1317
TseFI GTSAC 4 cut(s) 876, 1127, 1163, 1316
Tsp45I GTSAC 4 cut(s) 876, 1127, 1163, 1316
TspDTI ATGAA 4 cut(s) 16, 225, 1215, 1401
TspRI CASTG 5 cut(s) 190, 352, 1132, 1184, 1317
Van91I CCANNNNNTGG 2 cut(s) 358, 1160
VpaK11BI GGWCC 4 cut(s) 677, 732, 747, 1106
XagI CCTNNNNNAGG 1 cut(s) 64
XapI RAATTY 5 cut(s) 561, 906, 1007, 1187, 1272
XceI RCATGY 3 cut(s) 593, 604, 729
XmnI GAANNNNTTC 1 cut(s) 179
XspI CTAG 3 cut(s) 66, 1121, 1247
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.