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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 24 |
| AclWI | GGATC | 1 cut(s) | 78 |
| AgsI | TTSAA | 3 cut(s) | 67, 77, 113 |
| AleI | CACNNNNGTG | 1 cut(s) | 36 |
| Alw26I | GTCTC | 1 cut(s) | 92 |
| AlwI | GGATC | 1 cut(s) | 78 |
| BccI | CCATC | 1 cut(s) | 35 |
| BcoDI | GTCTC | 1 cut(s) | 92 |
| BfaI | CTAG | 1 cut(s) | 48 |
| BpuEI | CTTGAG | 1 cut(s) | 74 |
| BseGI | GGATG | 1 cut(s) | 27 |
| BslFI | GGGAC | 1 cut(s) | 29 |
| BsmAI | GTCTC | 1 cut(s) | 92 |
| BsmBI | CGTCTC | 1 cut(s) | 92 |
| BsmFI | GGGAC | 1 cut(s) | 29 |
| Bsp143I | GATC | 1 cut(s) | 70 |
| BspACI | CCGC | 1 cut(s) | 24 |
| BspPI | GGATC | 1 cut(s) | 78 |
| BssMI | GATC | 1 cut(s) | 70 |
| BstC8I | GCNNGC | 1 cut(s) | 60 |
| BstF5I | GGATG | 1 cut(s) | 27 |
| BstKTI | GATC | 1 cut(s) | 73 |
| BstMAI | GTCTC | 1 cut(s) | 92 |
| BstMBI | GATC | 1 cut(s) | 70 |
| BstX2I | RGATCY | 1 cut(s) | 70 |
| BstYI | RGATCY | 1 cut(s) | 70 |
| BtsCI | GGATG | 1 cut(s) | 27 |
| Cac8I | GCNNGC | 1 cut(s) | 60 |
| CviJI | RGCY | 1 cut(s) | 95 |
| CviKI_1 | RGCY | 1 cut(s) | 95 |
| DpnI | GATC | 1 cut(s) | 72 |
| DpnII | GATC | 1 cut(s) | 70 |
| Esp3I | CGTCTC | 1 cut(s) | 92 |
| FaiI | YATR | 1 cut(s) | 124 |
| FaqI | GGGAC | 1 cut(s) | 29 |
| FokI | GGATG | 1 cut(s) | 14 |
| FspBI | CTAG | 1 cut(s) | 48 |
| HpyCH4V | TGCA | 1 cut(s) | 4 |
| Kzo9I | GATC | 1 cut(s) | 70 |
| MaeI | CTAG | 1 cut(s) | 48 |
| MalI | GATC | 1 cut(s) | 72 |
| MboI | GATC | 1 cut(s) | 70 |
| MboII | GAAGA | 3 cut(s) | 63, 65, 74 |
| MflI | RGATCY | 1 cut(s) | 70 |
| MluCI | AATT | 1 cut(s) | 13 |
| MslI | CAYNNNNRTG | 1 cut(s) | 36 |
| NdeII | GATC | 1 cut(s) | 70 |
| OliI | CACNNNNGTG | 1 cut(s) | 36 |
| PsuI | RGATCY | 1 cut(s) | 70 |
| RseI | CAYNNNNRTG | 1 cut(s) | 36 |
| Sau3AI | GATC | 1 cut(s) | 70 |
| SetI | ASST | 1 cut(s) | 118 |
| SgeI | CNNG | 8 cut(s) | 48, 60, 71, 79, 89, 103, 125, 147 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 36 |
| SmlI | CTYRAG | 1 cut(s) | 89 |
| SmoI | CTYRAG | 1 cut(s) | 89 |
| Sse9I | AATT | 1 cut(s) | 13 |
| SsiI | CCGC | 1 cut(s) | 24 |
| SspMI | CTAG | 1 cut(s) | 48 |
| TasI | AATT | 1 cut(s) | 13 |
| TspDTI | ATGAA | 1 cut(s) | 94 |
| XspI | CTAG | 1 cut(s) | 48 |