Rroxscaffold_7G00192410

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0003963
Physical Location & Seq
Reverse (-)
33601334 .. 33603782
2449 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00192410.1

Sequence Viewer

Length: 552 bp
ATGCAATACATCAAGTTCCTATCCACCATCCACCCTTGTGTCGTTCCTAGAAGATTGCTTGCTTTCAAGGATCTTCAAGTTCTTCATCTCAAGGCTTGTCATCCATCTTTCACGGTTATATACAATTTAGCAGTGCTAGGAGGCCTCCGATTTTCTTGGAGAGATTTTGGAGTACGAAACCTAGGTTTCGTGTTGCTTTGCGACGATGTAGCTTCGCTCCCGGACCGAGGCAAGGCTGCTGCACGCTCTCGAGTGCGACGACGATGTGGCGGCGGTGCGGACTTTGGTCCGGATCATGTTTGGTTCGACGACGTCGGATTGGTTTCGGGTTTGTTCCAATCCGATTCCCTAGGTTGGTATAGATCGGGATGCGCGGTCGATCATGGTGGTGTCCGGTGTAGTTTTACGGGCATAGCACCGCGGCGGAGACTTTACGAGCGGGAGAACGGCAGCGGTAACGCTTCCGGAGAGGGAATTGATTGGCAAGACGAGTCGGGGTACCGGTTTCCGGCAAGTATTGCGGGGGATCGGATCCTCTCCGTCCGGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

183

Amino Acids

20.25

Weight (kDa)

9.21

Isoelectric Point (pI)

40.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 315
Acc65I GGTACC 1 cut(s) 498
AccB1I GGYRCC 1 cut(s) 498
AccBSI CCGCTC 1 cut(s) 439
AccII CGCG 2 cut(s) 374, 421
AccIII TCCGGA 2 cut(s) 289, 464
AclWI GGATC 5 cut(s) 78, 300, 526, 534, 539
AcyI GRCGYC 1 cut(s) 312
AfaI GTAC 2 cut(s) 174, 500
AfiI CCNNNNNNNGG 4 cut(s) 227, 232, 354, 508
AgeI ACCGGT 1 cut(s) 501
AgsI TTSAA 2 cut(s) 67, 77
AleI CACNNNNGTG 1 cut(s) 36
AluBI AGCT 1 cut(s) 212
AluI AGCT 1 cut(s) 212
Alw26I GTCTC 1 cut(s) 421
AlwI GGATC 5 cut(s) 78, 300, 526, 534, 539
Ama87I CYCGRG 1 cut(s) 249
Aor13HI TCCGGA 2 cut(s) 289, 464
AoxI GGCC 1 cut(s) 142
ApeKI GCWGC 3 cut(s) 236, 239, 450
AsiGI ACCGGT 1 cut(s) 501
Asp718I GGTACC 1 cut(s) 498
AspA2I CCTAGG 2 cut(s) 181, 349
AspLEI GCGC 1 cut(s) 374
AspS9I GGNCC 2 cut(s) 223, 287
AsuC2I CCSGG 2 cut(s) 221, 545
AvaI CYCGRG 1 cut(s) 249
AvaII GGWCC 2 cut(s) 223, 287
AvrII CCTAGG 2 cut(s) 181, 349
BamHI GGATCC 1 cut(s) 531
BanI GGYRCC 1 cut(s) 498
BbvI GCAGC 3 cut(s) 223, 226, 462
BccI CCATC 2 cut(s) 35, 112
BceAI ACGGC 1 cut(s) 463
BcnI CCSGG 2 cut(s) 221, 545
BcoDI GTCTC 1 cut(s) 421
BfaI CTAG 4 cut(s) 48, 137, 182, 350
BisI GCNGC 5 cut(s) 237, 240, 271, 422, 451
BlnI CCTAGG 2 cut(s) 181, 349
BlsI GCNGC 5 cut(s) 238, 241, 272, 423, 452
Bme1390I CCNGG 2 cut(s) 221, 545
Bme18I GGWCC 2 cut(s) 223, 287
BmeT110I CYCGRG 1 cut(s) 249
BmgT120I GGNCC 2 cut(s) 223, 287
BmiI GGNNCC 2 cut(s) 500, 533
BmrFI CCNGG 2 cut(s) 221, 545
BmsI GCATC 1 cut(s) 359
BoxI GACNNNNGTC 1 cut(s) 285
BpuEI CTTGAG 1 cut(s) 74
BpuMI CCSGG 2 cut(s) 221, 545
BsaHI GRCGYC 1 cut(s) 312
BsaJI CCNNGG 4 cut(s) 181, 226, 349, 419
BsaWI WCCGGW 4 cut(s) 289, 393, 464, 501
Bsc4I CCNNNNNNNGG 4 cut(s) 227, 232, 354, 508
Bse118I RCCGGY 1 cut(s) 501
BseAI TCCGGA 2 cut(s) 289, 464
BseDI CCNNGG 4 cut(s) 181, 226, 349, 419
BseGI GGATG 3 cut(s) 27, 100, 374
BseLI CCNNNNNNNGG 4 cut(s) 227, 232, 354, 508
BseXI GCAGC 3 cut(s) 223, 226, 462
BsgI GTGCAG 1 cut(s) 225
Bsh1236I CGCG 2 cut(s) 374, 421
Bsh1285I CGRYCG 1 cut(s) 378
BshFI GGCC 1 cut(s) 144
BshNI GGYRCC 1 cut(s) 498
BshTI ACCGGT 1 cut(s) 501
BsiEI CGRYCG 1 cut(s) 378
BsiHKCI CYCGRG 1 cut(s) 249
BsiSI CCGG 7 cut(s) 221, 290, 394, 465, 502, 509, 544
BslI CCNNNNNNNGG 4 cut(s) 227, 232, 354, 508
BsmAI GTCTC 1 cut(s) 421
BsnI GGCC 1 cut(s) 144
BsoBI CYCGRG 1 cut(s) 249
Bsp13I TCCGGA 2 cut(s) 289, 464
Bsp143I GATC 6 cut(s) 70, 292, 362, 379, 526, 531
BspANI GGCC 1 cut(s) 144
BspEI TCCGGA 2 cut(s) 289, 464
BspFNI CGCG 2 cut(s) 374, 421
BspLI GGNNCC 2 cut(s) 500, 533
BspPI GGATC 5 cut(s) 78, 300, 526, 534, 539
BspT107I GGYRCC 1 cut(s) 498
BsrBI CCGCTC 1 cut(s) 439
BsrFI RCCGGY 1 cut(s) 501
BssAI RCCGGY 1 cut(s) 501
BssECI CCNNGG 4 cut(s) 181, 226, 349, 419
BssMI GATC 6 cut(s) 70, 292, 362, 379, 526, 531
BssNI GRCGYC 1 cut(s) 312
BssT1I CCWWGG 2 cut(s) 181, 349
Bst4CI ACNGT 1 cut(s) 115
BstACI GRCGYC 1 cut(s) 312
BstAPI GCANNNNNTGC 1 cut(s) 518
BstC8I GCNNGC 2 cut(s) 60, 244
BstDSI CCRYGG 1 cut(s) 419
BstF5I GGATG 3 cut(s) 27, 100, 374
BstFNI CGCG 2 cut(s) 374, 421
BstHHI GCGC 1 cut(s) 374
BstKTI GATC 6 cut(s) 73, 295, 365, 382, 529, 534
BstMAI GTCTC 1 cut(s) 421
BstMBI GATC 6 cut(s) 70, 292, 362, 379, 526, 531
BstMCI CGRYCG 1 cut(s) 378
BstMWI GCNNNNNNNGC 1 cut(s) 518
BstPAI GACNNNNGTC 1 cut(s) 285
BstSCI CCNGG 2 cut(s) 219, 543
BstUI CGCG 2 cut(s) 374, 421
BstV1I GCAGC 3 cut(s) 223, 226, 462
BstX2I RGATCY 2 cut(s) 70, 531
BstYI RGATCY 2 cut(s) 70, 531
BsuRI GGCC 1 cut(s) 144
BtgI CCRYGG 1 cut(s) 419
BtsCI GGATG 3 cut(s) 27, 100, 374
BtsI GCAGTG 1 cut(s) 138
BtsIMutI CAGTG 1 cut(s) 138
Cac8I GCNNGC 2 cut(s) 60, 244
CfoI GCGC 1 cut(s) 374
Cfr10I RCCGGY 1 cut(s) 501
Cfr13I GGNCC 2 cut(s) 223, 287
Cfr42I CCGCGG 1 cut(s) 422
CpoI CGGWCCG 1 cut(s) 223
Csp6I GTAC 2 cut(s) 173, 499
CspAI ACCGGT 1 cut(s) 501
CspI CGGWCCG 1 cut(s) 223
CviAII CATG 2 cut(s) 296, 383
CviJI RGCY 4 cut(s) 95, 144, 212, 236
CviKI_1 RGCY 4 cut(s) 95, 144, 212, 236
CviQI GTAC 2 cut(s) 173, 499
DpnI GATC 6 cut(s) 72, 294, 364, 381, 528, 533
DpnII GATC 6 cut(s) 70, 292, 362, 379, 526, 531
EciI GGCGGA 1 cut(s) 439
Eco130I CCWWGG 2 cut(s) 181, 349
Eco147I AGGCCT 1 cut(s) 144
Eco47I GGWCC 2 cut(s) 223, 287
Eco88I CYCGRG 1 cut(s) 249
EcoT14I CCWWGG 2 cut(s) 181, 349
ErhI CCWWGG 2 cut(s) 181, 349
FaeI CATG 2 cut(s) 299, 386
FaiI YATR 6 cut(s) 119, 121, 297, 360, 384, 413
FatI CATG 2 cut(s) 295, 382
FauI CCCGC 2 cut(s) 432, 514
Fnu4HI GCNGC 5 cut(s) 237, 240, 271, 422, 451
FokI GGATG 3 cut(s) 14, 87, 381
Fsp4HI GCNGC 5 cut(s) 237, 240, 271, 422, 451
FspBI CTAG 4 cut(s) 48, 137, 182, 350
GlaI GCGC 1 cut(s) 373
GluI GCNGC 5 cut(s) 237, 240, 271, 422, 451
HaeIII GGCC 1 cut(s) 144
HapII CCGG 7 cut(s) 221, 290, 394, 465, 502, 509, 544
HhaI GCGC 1 cut(s) 374
Hin1I GRCGYC 1 cut(s) 312
Hin1II CATG 2 cut(s) 299, 386
Hin6I GCGC 1 cut(s) 372
HinP1I GCGC 1 cut(s) 372
HinfI GANTC 2 cut(s) 344, 491
HpaII CCGG 7 cut(s) 221, 290, 394, 465, 502, 509, 544
Hpy188I TCNGA 4 cut(s) 149, 317, 343, 531
Hpy188III TCNNGA 4 cut(s) 249, 290, 366, 465
Hpy99I CGWCG 6 cut(s) 206, 261, 264, 311, 314, 317
HpyCH4III ACNGT 1 cut(s) 115
HpyCH4IV ACGT 1 cut(s) 312
HpyCH4V TGCA 2 cut(s) 4, 242
HpyF10VI GCNNNNNNNGC 1 cut(s) 518
HpySE526I ACGT 1 cut(s) 312
Hsp92I GRCGYC 1 cut(s) 312
Hsp92II CATG 2 cut(s) 299, 386
HspAI GCGC 1 cut(s) 372
Kpn2I TCCGGA 2 cut(s) 289, 464
KpnI GGTACC 1 cut(s) 502
KspI CCGCGG 1 cut(s) 422
Kzo9I GATC 6 cut(s) 70, 292, 362, 379, 526, 531
LmnI GCTCC 1 cut(s) 222
LpnPI CCDG 6 cut(s) 234, 303, 407, 478, 515, 522
Lsp1109I GCAGC 3 cut(s) 223, 226, 462
LweI GCATC 1 cut(s) 359
MaeI CTAG 4 cut(s) 48, 137, 182, 350
MaeII ACGT 1 cut(s) 312
MaeIII GTNAC 1 cut(s) 455
MalI GATC 6 cut(s) 72, 294, 364, 381, 528, 533
MbiI CCGCTC 1 cut(s) 439
MboI GATC 6 cut(s) 70, 292, 362, 379, 526, 531
MboII GAAGA 3 cut(s) 63, 65, 74
MflI RGATCY 2 cut(s) 70, 531
MluCI AATT 2 cut(s) 124, 474
MlyI GAGTC 1 cut(s) 500
MmeI TCCRAC 1 cut(s) 295
MnlI CCTC 5 cut(s) 134, 155, 221, 463, 545
MroI TCCGGA 2 cut(s) 289, 464
MseI TTAA 1 cut(s) 550
MslI CAYNNNNRTG 2 cut(s) 36, 387
MspA1I CMGCKG 2 cut(s) 421, 453
MspI CCGG 7 cut(s) 221, 290, 394, 465, 502, 509, 544
MspR9I CCNGG 2 cut(s) 221, 545
MvnI CGCG 2 cut(s) 374, 421
MwoI GCNNNNNNNGC 1 cut(s) 518
NciI CCSGG 2 cut(s) 221, 545
NdeII GATC 6 cut(s) 70, 292, 362, 379, 526, 531
NlaIII CATG 2 cut(s) 299, 386
NlaIV GGNNCC 2 cut(s) 500, 533
OliI CACNNNNGTG 1 cut(s) 36
PaeR7I CTCGAG 1 cut(s) 249
PceI AGGCCT 1 cut(s) 144
PcsI WCGNNNNNNNCGW 1 cut(s) 256
PfeI GAWTC 1 cut(s) 344
PflFI GACNNNGTC 1 cut(s) 311
PfoI TCCNGGA 1 cut(s) 219
PinAI ACCGGT 1 cut(s) 501
PkrI GCNGC 5 cut(s) 238, 241, 272, 423, 452
PleI GAGTC 1 cut(s) 499
PpsI GAGTC 1 cut(s) 499
PshAI GACNNNNGTC 1 cut(s) 285
PspN4I GGNNCC 2 cut(s) 500, 533
PspPI GGNCC 2 cut(s) 223, 287
PsuI RGATCY 2 cut(s) 70, 531
PsyI GACNNNGTC 1 cut(s) 311
RsaI GTAC 2 cut(s) 174, 500
RsaNI GTAC 2 cut(s) 173, 499
RseI CAYNNNNRTG 2 cut(s) 36, 387
Rsr2I CGGWCCG 1 cut(s) 223
RsrII CGGWCCG 1 cut(s) 223
SacII CCGCGG 1 cut(s) 422
SaqAI TTAA 1 cut(s) 550
SatI GCNGC 5 cut(s) 237, 240, 271, 422, 451
Sau3AI GATC 6 cut(s) 70, 292, 362, 379, 526, 531
Sau96I GGNCC 2 cut(s) 223, 287
SchI GAGTC 1 cut(s) 500
ScrFI CCNGG 2 cut(s) 221, 545
SetI ASST 5 cut(s) 183, 187, 214, 315, 355
SfaNI GCATC 1 cut(s) 359
Sfr274I CTCGAG 1 cut(s) 249
Sfr303I CCGCGG 1 cut(s) 422
SgrBI CCGCGG 1 cut(s) 422
SinI GGWCC 2 cut(s) 223, 287
SlaI CTCGAG 1 cut(s) 249
SmiMI CAYNNNNRTG 2 cut(s) 36, 387
SmlI CTYRAG 2 cut(s) 89, 249
SmoI CTYRAG 2 cut(s) 89, 249
Sse9I AATT 2 cut(s) 124, 474
SseBI AGGCCT 1 cut(s) 144
SspMI CTAG 4 cut(s) 48, 137, 182, 350
StuI AGGCCT 1 cut(s) 144
StyD4I CCNGG 2 cut(s) 219, 543
StyI CCWWGG 2 cut(s) 181, 349
TaaI ACNGT 1 cut(s) 115
TaiI ACGT 1 cut(s) 315
TaqI TCGA 3 cut(s) 250, 306, 378
TaqII GACCGA 1 cut(s) 240
TasI AATT 2 cut(s) 124, 474
TauI GCSGC 2 cut(s) 273, 424
TfiI GAWTC 1 cut(s) 344
Tru1I TTAA 1 cut(s) 550
Tru9I TTAA 1 cut(s) 550
TscAI CASTG 1 cut(s) 138
TseI GCWGC 3 cut(s) 236, 239, 450
TspDTI ATGAA 1 cut(s) 74
TspGWI ACGGA 1 cut(s) 529
TspRI CASTG 1 cut(s) 138
Tth111I GACNNNGTC 1 cut(s) 311
VpaK11BI GGWCC 2 cut(s) 223, 287
XhoI CTCGAG 1 cut(s) 249
XmaJI CCTAGG 2 cut(s) 181, 349
XspI CTAG 4 cut(s) 48, 137, 182, 350
ZraI GACGTC 1 cut(s) 313
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.