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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclWI | GGATC | 1 cut(s) | 60 |
| AgsI | TTSAA | 3 cut(s) | 59, 79, 140 |
| AluBI | AGCT | 1 cut(s) | 41 |
| AluI | AGCT | 1 cut(s) | 41 |
| AlwI | GGATC | 1 cut(s) | 60 |
| BccI | CCATC | 4 cut(s) | 21, 27, 39, 83 |
| BceAI | ACGGC | 1 cut(s) | 124 |
| BfaI | CTAG | 1 cut(s) | 42 |
| BmiI | GGNNCC | 1 cut(s) | 112 |
| BseGI | GGATG | 1 cut(s) | 91 |
| Bsp143I | GATC | 2 cut(s) | 52, 100 |
| BspLI | GGNNCC | 1 cut(s) | 112 |
| BspPI | GGATC | 1 cut(s) | 60 |
| BssMI | GATC | 2 cut(s) | 52, 100 |
| Bst4CI | ACNGT | 1 cut(s) | 17 |
| BstC8I | GCNNGC | 1 cut(s) | 72 |
| BstF5I | GGATG | 1 cut(s) | 91 |
| BstKTI | GATC | 2 cut(s) | 55, 103 |
| BstMBI | GATC | 2 cut(s) | 52, 100 |
| BstX2I | RGATCY | 1 cut(s) | 52 |
| BstYI | RGATCY | 1 cut(s) | 52 |
| BtsCI | GGATG | 1 cut(s) | 91 |
| Cac8I | GCNNGC | 1 cut(s) | 72 |
| CviJI | RGCY | 3 cut(s) | 41, 74, 111 |
| CviKI_1 | RGCY | 3 cut(s) | 41, 74, 111 |
| DpnI | GATC | 2 cut(s) | 54, 102 |
| DpnII | GATC | 2 cut(s) | 52, 100 |
| FaiI | YATR | 1 cut(s) | 99 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 59, 91 |
| FokI | GGATG | 1 cut(s) | 98 |
| FspBI | CTAG | 1 cut(s) | 42 |
| HinfI | GANTC | 1 cut(s) | 59 |
| Hpy188III | TCNNGA | 1 cut(s) | 56 |
| HpyAV | CCTTC | 2 cut(s) | 85, 124 |
| HpyCH4III | ACNGT | 1 cut(s) | 17 |
| HpyCH4V | TGCA | 1 cut(s) | 146 |
| Kzo9I | GATC | 2 cut(s) | 52, 100 |
| LmnI | GCTCC | 1 cut(s) | 116 |
| MaeI | CTAG | 1 cut(s) | 42 |
| MalI | GATC | 2 cut(s) | 54, 102 |
| MboI | GATC | 2 cut(s) | 52, 100 |
| MboII | GAAGA | 4 cut(s) | 95, 109, 112, 115 |
| MflI | RGATCY | 1 cut(s) | 52 |
| MluCI | AATT | 1 cut(s) | 8 |
| MmeI | TCCRAC | 1 cut(s) | 30 |
| MnlI | CCTC | 2 cut(s) | 86, 137 |
| MseI | TTAA | 1 cut(s) | 154 |
| NdeII | GATC | 2 cut(s) | 52, 100 |
| NlaIV | GGNNCC | 1 cut(s) | 112 |
| PfeI | GAWTC | 1 cut(s) | 59 |
| PspN4I | GGNNCC | 1 cut(s) | 112 |
| PsuI | RGATCY | 1 cut(s) | 52 |
| SaqAI | TTAA | 1 cut(s) | 154 |
| Sau3AI | GATC | 2 cut(s) | 52, 100 |
| SetI | ASST | 2 cut(s) | 43, 84 |
| SgeI | CNNG | 9 cut(s) | 29, 54, 68, 75, 83, 91, 119, 145, 149 |
| Sse9I | AATT | 1 cut(s) | 8 |
| SspMI | CTAG | 1 cut(s) | 42 |
| TaaI | ACNGT | 1 cut(s) | 17 |
| TasI | AATT | 1 cut(s) | 8 |
| TfiI | GAWTC | 1 cut(s) | 59 |
| Tru1I | TTAA | 1 cut(s) | 154 |
| Tru9I | TTAA | 1 cut(s) | 154 |
| TspDTI | ATGAA | 1 cut(s) | 17 |
| XspI | CTAG | 1 cut(s) | 42 |