RchiOBHm_Chr5g0035741

Down syndrome critical region protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0002255
Physical Location & Seq
Forward (+)
29753012 .. 29754111
1100 bp
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UTR
Exon/CDS
Intron
PRQ31458

Sequence Viewer

Length: 441 bp
ATGAAACGGACGGCCGGAGTTATAGAGACTTTGGTTGGTGCTGCCAAGCCTATTTCGATTCTGAACAAAATTGTTGAGCTTAGACCTGCTGGAAAGATTGCTTCAGGCATAACCGAGATACCGTTTTCTATGCATATCAGGCAACCAAGAGAAGATAACCTTGAAAAGTTCTATGAGACATTCCATGGAGGAAATATTAACATTCAGTATTTAGTGACTGTAGATATATCGAGAGGATACCTGTATAAGTCGTTATCTACAACAATGGAGTTCATAGTTGAAAGTGATAAAGCTGATGTAATTGAGCGACCAGTTTCTCCGGAGATGGTTGTCTTTTACATTACTCAGGATACCCAAAGACATCCGCTACTTCCTCAACTGAAATCAGGTTTGCTGGTTACTTCCTGTTATATCCTATCTATCTCTAGTTACTGTTCTTAA

Protein Analysis

146

Amino Acids

16.38

Weight (kDa)

6.83

Isoelectric Point (pI)

56.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vps26 PF03643 6 - 94 4.5e-10 Vacuolar protein sorting-associated protein 26
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 94
AccIII TCCGGA 1 cut(s) 319
AciI CCGC 1 cut(s) 365
AcoI YGGCCR 1 cut(s) 12
AcuI CTGAAG 1 cut(s) 87
AgsI TTSAA 2 cut(s) 164, 281
AluBI AGCT 2 cut(s) 79, 293
AluI AGCT 2 cut(s) 79, 293
Alw26I GTCTC 2 cut(s) 20, 170
Aor13HI TCCGGA 1 cut(s) 319
AoxI GGCC 1 cut(s) 12
ApeKI GCWGC 1 cut(s) 41
BbvI GCAGC 1 cut(s) 28
BccI CCATC 1 cut(s) 319
BceAI ACGGC 1 cut(s) 27
BciVI GTATCC 2 cut(s) 230, 343
BcoDI GTCTC 2 cut(s) 20, 170
BfaI CTAG 1 cut(s) 426
BfmI CTRYAG 1 cut(s) 219
BfuAI ACCTGC 1 cut(s) 94
BfuI GTATCC 2 cut(s) 230, 343
BisI GCNGC 1 cut(s) 42
BlsI GCNGC 1 cut(s) 43
BsaJI CCNNGG 1 cut(s) 184
BsaWI WCCGGW 1 cut(s) 319
Bse1I ACTGG 1 cut(s) 311
BseAI TCCGGA 1 cut(s) 319
BseDI CCNNGG 1 cut(s) 184
BseGI GGATG 1 cut(s) 361
BseMII CTCAG 1 cut(s) 359
BseNI ACTGG 1 cut(s) 311
BseX3I CGGCCG 1 cut(s) 12
BseXI GCAGC 1 cut(s) 28
Bsh1285I CGRYCG 1 cut(s) 15
BshFI GGCC 1 cut(s) 14
BsiEI CGRYCG 1 cut(s) 15
BsiSI CCGG 2 cut(s) 15, 320
BsmAI GTCTC 2 cut(s) 20, 170
BsnI GGCC 1 cut(s) 14
Bsp13I TCCGGA 1 cut(s) 319
Bsp19I CCATGG 1 cut(s) 184
BspACI CCGC 1 cut(s) 365
BspANI GGCC 1 cut(s) 14
BspCNI CTCAG 1 cut(s) 358
BspEI TCCGGA 1 cut(s) 319
BspMI ACCTGC 1 cut(s) 94
BsrI ACTGG 1 cut(s) 311
BssECI CCNNGG 1 cut(s) 184
BssT1I CCWWGG 1 cut(s) 184
Bst4CI ACNGT 3 cut(s) 123, 220, 434
BstDEI CTNAG 2 cut(s) 80, 345
BstDSI CCRYGG 1 cut(s) 184
BstF5I GGATG 1 cut(s) 361
BstMAI GTCTC 2 cut(s) 20, 170
BstMCI CGRYCG 1 cut(s) 15
BstMWI GCNNNNNNNGC 1 cut(s) 139
BstSFI CTRYAG 1 cut(s) 219
BstV1I GCAGC 1 cut(s) 28
BstZI CGGCCG 1 cut(s) 12
BsuI GTATCC 2 cut(s) 230, 343
BsuRI GGCC 1 cut(s) 14
BtgI CCRYGG 1 cut(s) 184
BtsCI GGATG 1 cut(s) 361
BveI ACCTGC 1 cut(s) 94
CviAII CATG 1 cut(s) 185
CviJI RGCY 4 cut(s) 14, 49, 79, 293
CviKI_1 RGCY 4 cut(s) 14, 49, 79, 293
DdeI CTNAG 2 cut(s) 80, 345
EaeI YGGCCR 1 cut(s) 12
EagI CGGCCG 1 cut(s) 12
EclXI CGGCCG 1 cut(s) 12
Eco130I CCWWGG 1 cut(s) 184
Eco52I CGGCCG 1 cut(s) 12
Eco57I CTGAAG 1 cut(s) 87
EcoT14I CCWWGG 1 cut(s) 184
EcoT22I ATGCAT 1 cut(s) 135
ErhI CCWWGG 1 cut(s) 184
FaeI CATG 1 cut(s) 188
FalI AAGNNNNNCTT 2 cut(s) 144, 176
FatI CATG 1 cut(s) 184
Fnu4HI GCNGC 1 cut(s) 42
FokI GGATG 1 cut(s) 348
Fsp4HI GCNGC 1 cut(s) 42
FspBI CTAG 1 cut(s) 426
GluI GCNGC 1 cut(s) 42
HaeIII GGCC 1 cut(s) 14
HapII CCGG 2 cut(s) 15, 320
Hin1II CATG 1 cut(s) 188
HinfI GANTC 1 cut(s) 58
HpaII CCGG 2 cut(s) 15, 320
Hpy188I TCNGA 1 cut(s) 63
Hpy188III TCNNGA 3 cut(s) 231, 320, 347
HpyCH4III ACNGT 3 cut(s) 123, 220, 434
HpyCH4V TGCA 1 cut(s) 133
HpyF10VI GCNNNNNNNGC 1 cut(s) 139
HpyF3I CTNAG 2 cut(s) 80, 345
Hsp92II CATG 1 cut(s) 188
Kpn2I TCCGGA 1 cut(s) 319
Lsp1109I GCAGC 1 cut(s) 28
MaeI CTAG 1 cut(s) 426
MaeIII GTNAC 3 cut(s) 214, 397, 428
MboII GAAGA 1 cut(s) 164
MluCI AATT 2 cut(s) 69, 300
MnlI CCTC 3 cut(s) 182, 227, 384
Mph1103I ATGCAT 1 cut(s) 135
MroI TCCGGA 1 cut(s) 319
MseI TTAA 2 cut(s) 198, 439
MspI CCGG 2 cut(s) 15, 320
MwoI GCNNNNNNNGC 1 cut(s) 139
NcoI CCATGG 1 cut(s) 184
NlaIII CATG 1 cut(s) 188
NmuCI GTSAC 1 cut(s) 214
NsiI ATGCAT 1 cut(s) 135
PfeI GAWTC 1 cut(s) 58
PkrI GCNGC 1 cut(s) 43
SaqAI TTAA 2 cut(s) 198, 439
SatI GCNGC 1 cut(s) 42
SetI ASST 6 cut(s) 81, 88, 162, 243, 295, 391
SfcI CTRYAG 1 cut(s) 219
Sse9I AATT 2 cut(s) 69, 300
SsiI CCGC 1 cut(s) 365
SspI AATATT 1 cut(s) 196
SspMI CTAG 1 cut(s) 426
StyI CCWWGG 1 cut(s) 184
TaaI ACNGT 3 cut(s) 123, 220, 434
TaqI TCGA 2 cut(s) 56, 230
TasI AATT 2 cut(s) 69, 300
TfiI GAWTC 1 cut(s) 58
Tru1I TTAA 2 cut(s) 198, 439
Tru9I TTAA 2 cut(s) 198, 439
TseFI GTSAC 1 cut(s) 214
TseI GCWGC 1 cut(s) 41
Tsp45I GTSAC 1 cut(s) 214
TspDTI ATGAA 2 cut(s) 17, 262
TspGWI ACGGA 1 cut(s) 22
XspI CTAG 1 cut(s) 426
Zsp2I ATGCAT 1 cut(s) 135
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.