Rw1G006280

Down syndrome critical region protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0002255
Physical Location & Seq
Reverse (-)
12838450 .. 12842651
4202 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G006280.1

Sequence Viewer

Length: 765 bp
ATGTCGATTGAGCTCAAGCTTTCCCGGATTAATCGAGTTTATCGTCCTTCTGAACTGGTTGAGGGCAAGATCATAGTGAAGTCTGCGTCTTCAATCTCGCACTATGGAATTCGCATCGCGGTTGATGGATCTGTCAACTTGCAGGTTCGGGGAGGGACGGCCAGAGTTATAGAGACTTTGGTTGGTGCTGCCAAGCCTATTTCGATTCTCAGGAACAAAATTGTTGAGCTTAGACCTGCTGGAAAGATTGCTTCAGGCATAACCGAGATACCGTTTTCTATGCATATCAGGCAACCAAGAGAAGATAACCTCGAAAAGTTCTATGAGACATTCCATGGAGGAAATATTAACATTCAGTATTTAGTGACTGTAGATATATCGAGAGGATACCTGTATAAGTCGTTATCTACAACAATGGAGTTCATAGTTGAAAGTGATAAAGGTATACATCCGCTACTTCCTCAACTGAAATCAGCAGCAATTGGAGAACTTGAAGATGAAGATAATGCCGGGAAGAATCAGCTGCATGTTGATGCAGTGAAGAATGTTACAGAGGAAATAGAATCAGTCGATTTGACCCTCCTAGATGTGCTCCATATTTTGGTGTTGGAGTATACAATTATGTGGATTATAACAATATTAGATATATATATATTGGAAATTATTGTTCATTTGGTAATTTTTTATTTCATTTTCTGTTCATTTCATTCATCTAAGGATCCAAGAATTTCACTAGCCCTAAATTCAATACCCATGTATTGGTAG

Protein Analysis

254

Amino Acids

28.65

Weight (kDa)

5.92

Isoelectric Point (pI)

42.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vps26 PF03643 11 - 146 1.6e-13 Vacuolar protein sorting-associated protein 26
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 632
Acc36I ACCTGC 2 cut(s) 133, 244
AccB7I CCANNNNNTGG 2 cut(s) 601, 759
AccI GTMKAC 2 cut(s) 445, 614
AccII CGCG 1 cut(s) 119
AciI CCGC 2 cut(s) 119, 452
AclWI GGATC 3 cut(s) 136, 713, 726
AcoI YGGCCR 1 cut(s) 159
AcsI RAATTY 3 cut(s) 108, 726, 742
AcuI CTGAAG 1 cut(s) 237
AfiI CCNNNNNNNGG 2 cut(s) 601, 759
AgsI TTSAA 4 cut(s) 93, 431, 494, 747
AluBI AGCT 4 cut(s) 13, 19, 229, 523
AluI AGCT 4 cut(s) 13, 19, 229, 523
Alw21I GWGCWC 2 cut(s) 15, 594
Alw26I GTCTC 2 cut(s) 167, 320
AlwI GGATC 3 cut(s) 136, 713, 726
AoxI GGCC 1 cut(s) 159
ApeKI GCWGC 3 cut(s) 188, 476, 523
ApoI RAATTY 3 cut(s) 108, 726, 742
AseI ATTAAT 1 cut(s) 30
AsuC2I CCSGG 2 cut(s) 25, 511
BamHI GGATCC 1 cut(s) 718
BanII GRGCYC 1 cut(s) 15
BbsI GAAGAC 1 cut(s) 81
Bbv12I GWGCWC 2 cut(s) 15, 594
BbvI GCAGC 3 cut(s) 175, 488, 510
BccI CCATC 1 cut(s) 119
BceAI ACGGC 1 cut(s) 174
BciVI GTATCC 1 cut(s) 380
BcnI CCSGG 2 cut(s) 25, 511
BcoDI GTCTC 2 cut(s) 167, 320
BfaI CTAG 2 cut(s) 584, 734
BfmI CTRYAG 1 cut(s) 369
BfuAI ACCTGC 2 cut(s) 133, 244
BfuI GTATCC 1 cut(s) 380
BisI GCNGC 3 cut(s) 189, 477, 524
BlsI GCNGC 3 cut(s) 190, 478, 525
Bme1390I CCNGG 2 cut(s) 25, 511
BmiI GGNNCC 1 cut(s) 720
BmrFI CCNGG 2 cut(s) 25, 511
BmsI GCATC 2 cut(s) 123, 523
BpiI GAAGAC 1 cut(s) 81
BpuMI CCSGG 2 cut(s) 25, 511
BsaJI CCNNGG 1 cut(s) 334
Bsc4I CCNNNNNNNGG 2 cut(s) 601, 759
Bse1I ACTGG 1 cut(s) 60
BseDI CCNNGG 1 cut(s) 334
BseGI GGATG 1 cut(s) 448
BseLI CCNNNNNNNGG 2 cut(s) 601, 759
BseMII CTCAG 1 cut(s) 223
BseNI ACTGG 1 cut(s) 60
BseXI GCAGC 3 cut(s) 175, 488, 510
Bsh1236I CGCG 1 cut(s) 119
BshFI GGCC 1 cut(s) 161
BsiHKAI GWGCWC 2 cut(s) 15, 594
BsiSI CCGG 2 cut(s) 25, 510
BslFI GGGAC 1 cut(s) 169
BslI CCNNNNNNNGG 2 cut(s) 601, 759
BsmAI GTCTC 2 cut(s) 167, 320
BsmFI GGGAC 1 cut(s) 169
BsnI GGCC 1 cut(s) 161
Bsp1286I GDGCHC 2 cut(s) 15, 594
Bsp143I GATC 3 cut(s) 69, 128, 718
Bsp19I CCATGG 1 cut(s) 334
BspACI CCGC 2 cut(s) 119, 452
BspANI GGCC 1 cut(s) 161
BspCNI CTCAG 1 cut(s) 222
BspFNI CGCG 1 cut(s) 119
BspLI GGNNCC 1 cut(s) 720
BspMI ACCTGC 2 cut(s) 133, 244
BspPI GGATC 3 cut(s) 136, 713, 726
BsrI ACTGG 1 cut(s) 60
BssECI CCNNGG 1 cut(s) 334
BssMI GATC 3 cut(s) 69, 128, 718
BssNAI GTATAC 2 cut(s) 446, 615
BssT1I CCWWGG 1 cut(s) 334
Bst1107I GTATAC 2 cut(s) 446, 615
Bst4CI ACNGT 2 cut(s) 273, 370
BstDEI CTNAG 3 cut(s) 209, 230, 714
BstDSI CCRYGG 1 cut(s) 334
BstF5I GGATG 1 cut(s) 448
BstFNI CGCG 1 cut(s) 119
BstKTI GATC 3 cut(s) 72, 131, 721
BstMAI GTCTC 2 cut(s) 167, 320
BstMBI GATC 3 cut(s) 69, 128, 718
BstMWI GCNNNNNNNGC 1 cut(s) 289
BstNSI RCATGY 1 cut(s) 530
BstSCI CCNGG 2 cut(s) 23, 509
BstSFI CTRYAG 1 cut(s) 369
BstUI CGCG 1 cut(s) 119
BstV1I GCAGC 3 cut(s) 175, 488, 510
BstV2I GAAGAC 1 cut(s) 81
BstX2I RGATCY 2 cut(s) 128, 718
BstYI RGATCY 2 cut(s) 128, 718
BstZ17I GTATAC 2 cut(s) 446, 615
BsuI GTATCC 1 cut(s) 380
BsuRI GGCC 1 cut(s) 161
BtgI CCRYGG 1 cut(s) 334
BtgZI GCGATG 1 cut(s) 100
BtsCI GGATG 1 cut(s) 448
BtsI GCAGTG 1 cut(s) 543
BtsIMutI CAGTG 1 cut(s) 543
BveI ACCTGC 2 cut(s) 133, 244
CseI GACGC 1 cut(s) 75
CviAII CATG 3 cut(s) 335, 527, 754
CviJI RGCY 7 cut(s) 13, 19, 161, 196, 229, 523, 737
CviKI_1 RGCY 7 cut(s) 13, 19, 161, 196, 229, 523, 737
DdeI CTNAG 3 cut(s) 209, 230, 714
DpnI GATC 3 cut(s) 71, 130, 720
DpnII GATC 3 cut(s) 69, 128, 718
EaeI YGGCCR 1 cut(s) 159
Ecl136II GAGCTC 1 cut(s) 13
Eco130I CCWWGG 1 cut(s) 334
Eco24I GRGCYC 1 cut(s) 15
Eco53kI GAGCTC 1 cut(s) 13
Eco57I CTGAAG 1 cut(s) 237
EcoICRI GAGCTC 1 cut(s) 13
EcoRI GAATTC 1 cut(s) 108
EcoT14I CCWWGG 1 cut(s) 334
EcoT22I ATGCAT 1 cut(s) 285
EcoT38I GRGCYC 1 cut(s) 15
ErhI CCWWGG 1 cut(s) 334
FaeI CATG 3 cut(s) 338, 530, 757
FaqI GGGAC 1 cut(s) 169
FatI CATG 3 cut(s) 334, 526, 753
FblI GTMKAC 2 cut(s) 445, 614
Fnu4HI GCNGC 3 cut(s) 189, 477, 524
FokI GGATG 1 cut(s) 435
FriOI GRGCYC 1 cut(s) 15
Fsp4HI GCNGC 3 cut(s) 189, 477, 524
FspBI CTAG 2 cut(s) 584, 734
GluI GCNGC 3 cut(s) 189, 477, 524
HaeIII GGCC 1 cut(s) 161
HapII CCGG 2 cut(s) 25, 510
HgaI GACGC 1 cut(s) 75
Hin1II CATG 3 cut(s) 338, 530, 757
HincII GTYRAC 1 cut(s) 136
HindII GTYRAC 1 cut(s) 136
HindIII AAGCTT 1 cut(s) 17
HinfI GANTC 3 cut(s) 205, 517, 563
HpaII CCGG 2 cut(s) 25, 510
Hpy166II GTNNAC 3 cut(s) 136, 446, 615
Hpy188I TCNGA 1 cut(s) 52
Hpy188III TCNNGA 2 cut(s) 211, 381
Hpy8I GTNNAC 3 cut(s) 136, 446, 615
HpyAV CCTTC 1 cut(s) 57
HpyCH4III ACNGT 2 cut(s) 273, 370
HpyCH4V TGCA 4 cut(s) 142, 283, 526, 536
HpyF10VI GCNNNNNNNGC 1 cut(s) 289
HpyF3I CTNAG 3 cut(s) 209, 230, 714
Hsp92II CATG 3 cut(s) 338, 530, 757
Kzo9I GATC 3 cut(s) 69, 128, 718
LmnI GCTCC 1 cut(s) 597
Lsp1109I GCAGC 3 cut(s) 175, 488, 510
LweI GCATC 2 cut(s) 123, 523
MaeI CTAG 2 cut(s) 584, 734
MaeIII GTNAC 2 cut(s) 364, 547
MalI GATC 3 cut(s) 71, 130, 720
MboI GATC 3 cut(s) 69, 128, 718
MboII GAAGA 6 cut(s) 81, 314, 506, 512, 526, 553
MfeI CAATTG 1 cut(s) 480
MflI RGATCY 2 cut(s) 128, 718
MhlI GDGCHC 2 cut(s) 15, 594
MluCI AATT 8 cut(s) 108, 219, 480, 618, 660, 678, 726, 742
MmeI TCCRAC 1 cut(s) 588
MnlI CCTC 8 cut(s) 55, 146, 320, 332, 377, 471, 547, 590
Mph1103I ATGCAT 1 cut(s) 285
MseI TTAA 2 cut(s) 30, 348
MslI CAYNNNNRTG 1 cut(s) 531
MspA1I CMGCKG 1 cut(s) 523
MspI CCGG 2 cut(s) 25, 510
MspR9I CCNGG 2 cut(s) 25, 511
MunI CAATTG 1 cut(s) 480
MvnI CGCG 1 cut(s) 119
MwoI GCNNNNNNNGC 1 cut(s) 289
NciI CCSGG 2 cut(s) 25, 511
NcoI CCATGG 1 cut(s) 334
NdeII GATC 3 cut(s) 69, 128, 718
NlaIII CATG 3 cut(s) 338, 530, 757
NlaIV GGNNCC 1 cut(s) 720
NmuCI GTSAC 1 cut(s) 364
NsiI ATGCAT 1 cut(s) 285
NspI RCATGY 1 cut(s) 530
PcsI WCGNNNNNNNCGW 1 cut(s) 40
PfeI GAWTC 3 cut(s) 205, 517, 563
PflMI CCANNNNNTGG 2 cut(s) 601, 759
PfoI TCCNGGA 1 cut(s) 23
PkrI GCNGC 3 cut(s) 190, 478, 525
PshBI ATTAAT 1 cut(s) 30
PsiI TTATAA 1 cut(s) 632
Psp124BI GAGCTC 1 cut(s) 15
PspN4I GGNNCC 1 cut(s) 720
PsuI RGATCY 2 cut(s) 128, 718
PvuII CAGCTG 1 cut(s) 523
RseI CAYNNNNRTG 1 cut(s) 531
SacI GAGCTC 1 cut(s) 15
SaqAI TTAA 2 cut(s) 30, 348
SatI GCNGC 3 cut(s) 189, 477, 524
Sau3AI GATC 3 cut(s) 69, 128, 718
ScrFI CCNGG 2 cut(s) 25, 511
SduI GDGCHC 2 cut(s) 15, 594
SetI ASST 9 cut(s) 15, 21, 147, 231, 238, 312, 393, 445, 525
SfaNI GCATC 2 cut(s) 123, 523
SfcI CTRYAG 1 cut(s) 369
SmiMI CAYNNNNRTG 1 cut(s) 531
SmlI CTYRAG 1 cut(s) 14
SmoI CTYRAG 1 cut(s) 14
Sse9I AATT 8 cut(s) 108, 219, 480, 618, 660, 678, 726, 742
SsiI CCGC 2 cut(s) 119, 452
SspI AATATT 2 cut(s) 346, 639
SspMI CTAG 2 cut(s) 584, 734
SstI GAGCTC 1 cut(s) 15
StyD4I CCNGG 2 cut(s) 23, 509
StyI CCWWGG 1 cut(s) 334
TaaI ACNGT 2 cut(s) 273, 370
TaqI TCGA 6 cut(s) 5, 34, 203, 312, 380, 570
TasI AATT 8 cut(s) 108, 219, 480, 618, 660, 678, 726, 742
TfiI GAWTC 3 cut(s) 205, 517, 563
Tru1I TTAA 2 cut(s) 30, 348
Tru9I TTAA 2 cut(s) 30, 348
TscAI CASTG 1 cut(s) 543
TseFI GTSAC 1 cut(s) 364
TseI GCWGC 3 cut(s) 188, 476, 523
Tsp45I GTSAC 1 cut(s) 364
TspDTI ATGAA 7 cut(s) 412, 513, 659, 679, 690, 695, 699
TspRI CASTG 1 cut(s) 543
Van91I CCANNNNNTGG 2 cut(s) 601, 759
VspI ATTAAT 1 cut(s) 30
XapI RAATTY 3 cut(s) 108, 726, 742
XceI RCATGY 1 cut(s) 530
XmiI GTMKAC 2 cut(s) 445, 614
XspI CTAG 2 cut(s) 584, 734
Zsp2I ATGCAT 1 cut(s) 285
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.