Rroxscaffold_3G00275030

Down syndrome critical region protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0002255
Physical Location & Seq
Forward (+)
66589132 .. 66591851
2720 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00275030.1

Sequence Viewer

Length: 891 bp
ATGTCGATTGAGCTCAAGCTTTCCCGGATTAATCGAGTTTATCGTCCTTCTGAACTGGTTGAGGGCAAGATCGTAGTGAAGTCTTCGTCTTCAATCTCGCACTATGGAATTCGCATCGCGGTTGATGGATCTGTCAACTTGCAGGTTCGGGGAGGGACGGCCGGAGTTATAGAGACTTTGGTTGGAGCTGCCAAGCCTATTTCGATTCTCGTTTTCTCACTTGAAAGCAGGAACAAAATTGTTGAGCTTAGACCTGCTGGAAAGATTGCTTCAGGCATAACCGAGGTGCATCTTTCTGCCTTGGAAAATATATCGAGAGGATACCTGTATAAGTCGTTATCTACAACAATGGAGTTCATAGTTGAAAGTGATAAAGCTGATGTAATTGAGCGACCAGTTTCTCCGGAGATGGTTGTCTTTTACATTACTCAGGATACCCAAAGACATCCGCTACTTCCTCAACTGAAATCAGGTGGATTTCGAATCATTGGAAAAATGGCCACTCAATGTTCTTTATCAGATCCTGTTAGTGGTGAACTAACAGTAGAAGCATCTGCAGTTCCAATTTCTTCCATTGATATACACTTGCTTCGTGTAGAGTCAATCCTTCTAGGGGAGAAAATTGTGACTGAGAAGTCTGTGGTTCAGACTACACAGATAGCAGATGGAGACGTCTGTCATAATATGACTCTGCCCATTCATGTTATACTTCCCCGTCTTTTGACATGTCCTACAGTCTCAGCAGGGCCATTCTCGATTGAATTTAAAGTTGTCATAGTCATAACATTTCAGTCAGAGGTAGCCAAATTGCATTCAAAATCTGATCCTACAACTCCCAGACTATGGCTTGCAATGGAGACTCTACCGCTTGAACTTGTTAGAACAAGGTAA

Protein Analysis

296

Amino Acids

32.32

Weight (kDa)

7.76

Isoelectric Point (pI)

43.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vps26 PF03643 171 - 262 7.2e-12 Vacuolar protein sorting-associated protein 26
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 634
AatII GACGTC 1 cut(s) 675
Acc36I ACCTGC 2 cut(s) 133, 262
AccB7I CCANNNNNTGG 1 cut(s) 843
AccII CGCG 1 cut(s) 119
AccIII TCCGGA 1 cut(s) 403
AciI CCGC 3 cut(s) 119, 449, 866
AclWI GGATC 3 cut(s) 136, 515, 818
AcoI YGGCCR 2 cut(s) 159, 498
AcsI RAATTY 2 cut(s) 108, 761
AcuI CTGAAG 1 cut(s) 255
AcyI GRCGYC 1 cut(s) 672
AfiI CCNNNNNNNGG 3 cut(s) 530, 613, 843
AflIII ACRYGT 1 cut(s) 725
AgsI TTSAA 6 cut(s) 93, 224, 365, 761, 816, 872
AluBI AGCT 5 cut(s) 13, 19, 188, 247, 377
AluI AGCT 5 cut(s) 13, 19, 188, 247, 377
Alw21I GWGCWC 1 cut(s) 15
Alw26I GTCTC 4 cut(s) 167, 663, 742, 851
AlwI GGATC 3 cut(s) 136, 515, 818
AlwNI CAGNNNCTG 1 cut(s) 524
Aor13HI TCCGGA 1 cut(s) 403
AoxI GGCC 3 cut(s) 159, 498, 746
ApeKI GCWGC 1 cut(s) 188
ApoI RAATTY 2 cut(s) 108, 761
AseI ATTAAT 1 cut(s) 30
AspS9I GGNCC 1 cut(s) 746
AsuC2I CCSGG 1 cut(s) 25
AsuHPI GGTGA 1 cut(s) 545
AsuII TTCGAA 1 cut(s) 481
BalI TGGCCA 1 cut(s) 500
BanII GRGCYC 1 cut(s) 15
BbsI GAAGAC 2 cut(s) 75, 81
Bbv12I GWGCWC 1 cut(s) 15
BbvI GCAGC 1 cut(s) 175
BccI CCATC 3 cut(s) 119, 403, 659
BceAI ACGGC 1 cut(s) 174
BciVI GTATCC 2 cut(s) 314, 427
BcnI CCSGG 1 cut(s) 25
BcoDI GTCTC 4 cut(s) 167, 663, 742, 851
BfaI CTAG 1 cut(s) 611
BfmI CTRYAG 2 cut(s) 555, 732
BfuAI ACCTGC 2 cut(s) 133, 262
BfuI GTATCC 2 cut(s) 314, 427
BisI GCNGC 1 cut(s) 189
BlsI GCNGC 1 cut(s) 190
Bme1390I CCNGG 1 cut(s) 25
BmgT120I GGNCC 1 cut(s) 746
BmrFI CCNGG 1 cut(s) 25
BmsI GCATC 3 cut(s) 123, 298, 560
BoxI GACNNNNGTC 1 cut(s) 675
BpiI GAAGAC 2 cut(s) 75, 81
Bpu14I TTCGAA 1 cut(s) 481
BpuMI CCSGG 1 cut(s) 25
BsaHI GRCGYC 1 cut(s) 672
BsaJI CCNNGG 2 cut(s) 282, 300
BsaWI WCCGGW 1 cut(s) 403
Bsc4I CCNNNNNNNGG 3 cut(s) 530, 613, 843
Bse1I ACTGG 2 cut(s) 60, 395
Bse3DI GCAATG 1 cut(s) 858
BseAI TCCGGA 1 cut(s) 403
BseDI CCNNGG 2 cut(s) 282, 300
BseGI GGATG 1 cut(s) 445
BseLI CCNNNNNNNGG 3 cut(s) 530, 613, 843
BseMI GCAATG 1 cut(s) 858
BseMII CTCAG 3 cut(s) 443, 621, 753
BseNI ACTGG 2 cut(s) 60, 395
BseX3I CGGCCG 1 cut(s) 159
BseXI GCAGC 1 cut(s) 175
Bsh1236I CGCG 1 cut(s) 119
Bsh1285I CGRYCG 1 cut(s) 162
BshFI GGCC 3 cut(s) 161, 500, 748
BsiEI CGRYCG 1 cut(s) 162
BsiHKAI GWGCWC 1 cut(s) 15
BsiSI CCGG 3 cut(s) 25, 162, 404
BslFI GGGAC 1 cut(s) 169
BslI CCNNNNNNNGG 3 cut(s) 530, 613, 843
BsmAI GTCTC 4 cut(s) 167, 663, 742, 851
BsmBI CGTCTC 1 cut(s) 663
BsmFI GGGAC 1 cut(s) 169
BsmI GAATGC 1 cut(s) 811
BsnI GGCC 3 cut(s) 161, 500, 748
Bsp119I TTCGAA 1 cut(s) 481
Bsp1286I GDGCHC 1 cut(s) 15
Bsp13I TCCGGA 1 cut(s) 403
Bsp143I GATC 4 cut(s) 69, 128, 520, 823
BspACI CCGC 3 cut(s) 119, 449, 866
BspANI GGCC 3 cut(s) 161, 500, 748
BspCNI CTCAG 3 cut(s) 442, 622, 752
BspEI TCCGGA 1 cut(s) 403
BspFNI CGCG 1 cut(s) 119
BspMAI CTGCAG 1 cut(s) 559
BspMI ACCTGC 2 cut(s) 133, 262
BspPI GGATC 3 cut(s) 136, 515, 818
BspT104I TTCGAA 1 cut(s) 481
BsrDI GCAATG 1 cut(s) 858
BsrI ACTGG 2 cut(s) 60, 395
BssECI CCNNGG 2 cut(s) 282, 300
BssMI GATC 4 cut(s) 69, 128, 520, 823
BssNI GRCGYC 1 cut(s) 672
BssT1I CCWWGG 1 cut(s) 300
Bst4CI ACNGT 2 cut(s) 544, 736
BstACI GRCGYC 1 cut(s) 672
BstBI TTCGAA 1 cut(s) 481
BstC8I GCNNGC 1 cut(s) 849
BstDEI CTNAG 4 cut(s) 248, 429, 630, 739
BstF5I GGATG 1 cut(s) 445
BstFNI CGCG 1 cut(s) 119
BstKTI GATC 4 cut(s) 72, 131, 523, 826
BstMAI GTCTC 4 cut(s) 167, 663, 742, 851
BstMBI GATC 4 cut(s) 69, 128, 520, 823
BstMCI CGRYCG 1 cut(s) 162
BstNSI RCATGY 1 cut(s) 729
BstPAI GACNNNNGTC 1 cut(s) 675
BstSCI CCNGG 1 cut(s) 23
BstSFI CTRYAG 2 cut(s) 555, 732
BstUI CGCG 1 cut(s) 119
BstV1I GCAGC 1 cut(s) 175
BstV2I GAAGAC 2 cut(s) 75, 81
BstX2I RGATCY 2 cut(s) 128, 520
BstYI RGATCY 2 cut(s) 128, 520
BstZI CGGCCG 1 cut(s) 159
BsuI GTATCC 2 cut(s) 314, 427
BsuRI GGCC 3 cut(s) 161, 500, 748
BtgZI GCGATG 1 cut(s) 100
BtsCI GGATG 1 cut(s) 445
BveI ACCTGC 2 cut(s) 133, 262
Cac8I GCNNGC 1 cut(s) 849
CaiI CAGNNNCTG 1 cut(s) 524
Cfr13I GGNCC 1 cut(s) 746
CviAII CATG 2 cut(s) 701, 726
DdeI CTNAG 4 cut(s) 248, 429, 630, 739
DpnI GATC 4 cut(s) 71, 130, 522, 825
DpnII GATC 4 cut(s) 69, 128, 520, 823
DraI TTTAAA 1 cut(s) 766
DrdI GACNNNNNNGTC 1 cut(s) 634
DseDI GACNNNNNNGTC 1 cut(s) 634
EaeI YGGCCR 2 cut(s) 159, 498
EagI CGGCCG 1 cut(s) 159
Ecl136II GAGCTC 1 cut(s) 13
EclXI CGGCCG 1 cut(s) 159
Eco130I CCWWGG 1 cut(s) 300
Eco24I GRGCYC 1 cut(s) 15
Eco52I CGGCCG 1 cut(s) 159
Eco53kI GAGCTC 1 cut(s) 13
Eco57I CTGAAG 1 cut(s) 255
EcoICRI GAGCTC 1 cut(s) 13
EcoRI GAATTC 1 cut(s) 108
EcoT14I CCWWGG 1 cut(s) 300
EcoT38I GRGCYC 1 cut(s) 15
ErhI CCWWGG 1 cut(s) 300
Esp3I CGTCTC 1 cut(s) 663
FaeI CATG 2 cut(s) 704, 729
FaqI GGGAC 1 cut(s) 169
FatI CATG 2 cut(s) 700, 725
Fnu4HI GCNGC 1 cut(s) 189
FokI GGATG 1 cut(s) 432
FriOI GRGCYC 1 cut(s) 15
Fsp4HI GCNGC 1 cut(s) 189
FspBI CTAG 1 cut(s) 611
GluI GCNGC 1 cut(s) 189
HaeIII GGCC 3 cut(s) 161, 500, 748
HapII CCGG 3 cut(s) 25, 162, 404
Hin1I GRCGYC 1 cut(s) 672
Hin1II CATG 2 cut(s) 704, 729
HincII GTYRAC 1 cut(s) 136
HindII GTYRAC 1 cut(s) 136
HindIII AAGCTT 1 cut(s) 17
HinfI GANTC 5 cut(s) 205, 483, 599, 688, 859
HpaII CCGG 3 cut(s) 25, 162, 404
HphI GGTGA 1 cut(s) 545
Hpy166II GTNNAC 2 cut(s) 136, 536
Hpy188I TCNGA 5 cut(s) 52, 520, 648, 796, 823
Hpy188III TCNNGA 4 cut(s) 315, 404, 431, 754
Hpy8I GTNNAC 2 cut(s) 136, 536
HpyAV CCTTC 2 cut(s) 57, 617
HpyCH4III ACNGT 2 cut(s) 544, 736
HpyCH4IV ACGT 1 cut(s) 672
HpyCH4V TGCA 5 cut(s) 142, 289, 557, 811, 851
HpyF3I CTNAG 4 cut(s) 248, 429, 630, 739
HpySE526I ACGT 1 cut(s) 672
Hsp92I GRCGYC 1 cut(s) 672
Hsp92II CATG 2 cut(s) 704, 729
Kpn2I TCCGGA 1 cut(s) 403
Kzo9I GATC 4 cut(s) 69, 128, 520, 823
LmnI GCTCC 1 cut(s) 185
Lsp1109I GCAGC 1 cut(s) 175
LweI GCATC 3 cut(s) 123, 298, 560
MaeI CTAG 1 cut(s) 611
MaeII ACGT 1 cut(s) 672
MaeIII GTNAC 1 cut(s) 625
MalI GATC 4 cut(s) 71, 130, 522, 825
MboI GATC 4 cut(s) 69, 128, 520, 823
MboII GAAGA 3 cut(s) 75, 81, 561
MflI RGATCY 2 cut(s) 128, 520
MhlI GDGCHC 1 cut(s) 15
MlsI TGGCCA 1 cut(s) 500
MluCI AATT 7 cut(s) 108, 237, 384, 564, 621, 761, 806
MluNI TGGCCA 1 cut(s) 500
MlyI GAGTC 3 cut(s) 608, 682, 853
MmeI TCCRAC 1 cut(s) 163
MnlI CCTC 6 cut(s) 55, 146, 277, 311, 468, 790
Mox20I TGGCCA 1 cut(s) 500
MroI TCCGGA 1 cut(s) 403
MscI TGGCCA 1 cut(s) 500
MseI TTAA 2 cut(s) 30, 765
Msp20I TGGCCA 1 cut(s) 500
MspI CCGG 3 cut(s) 25, 162, 404
MspR9I CCNGG 1 cut(s) 25
Mva1269I GAATGC 1 cut(s) 811
MvnI CGCG 1 cut(s) 119
NciI CCSGG 1 cut(s) 25
NdeII GATC 4 cut(s) 69, 128, 520, 823
NlaIII CATG 2 cut(s) 704, 729
NmuCI GTSAC 1 cut(s) 625
NspI RCATGY 1 cut(s) 729
NspV TTCGAA 1 cut(s) 481
PciI ACATGT 1 cut(s) 725
PcsI WCGNNNNNNNCGW 1 cut(s) 40
PctI GAATGC 1 cut(s) 811
PfeI GAWTC 2 cut(s) 205, 483
PflMI CCANNNNNTGG 1 cut(s) 843
PfoI TCCNGGA 1 cut(s) 23
PkrI GCNGC 1 cut(s) 190
PleI GAGTC 3 cut(s) 607, 682, 853
PpsI GAGTC 3 cut(s) 607, 682, 853
PscI ACATGT 1 cut(s) 725
PshAI GACNNNNGTC 1 cut(s) 675
PshBI ATTAAT 1 cut(s) 30
Psp124BI GAGCTC 1 cut(s) 15
PspPI GGNCC 1 cut(s) 746
PstI CTGCAG 1 cut(s) 559
PstNI CAGNNNCTG 1 cut(s) 524
PsuI RGATCY 2 cut(s) 128, 520
SacI GAGCTC 1 cut(s) 15
SaqAI TTAA 2 cut(s) 30, 765
SatI GCNGC 1 cut(s) 189
Sau3AI GATC 4 cut(s) 69, 128, 520, 823
Sau96I GGNCC 1 cut(s) 746
SchI GAGTC 3 cut(s) 608, 682, 853
ScrFI CCNGG 1 cut(s) 25
SduI GDGCHC 1 cut(s) 15
SfaNI GCATC 3 cut(s) 123, 298, 560
SfcI CTRYAG 2 cut(s) 555, 732
SfuI TTCGAA 1 cut(s) 481
SmlI CTYRAG 1 cut(s) 14
SmoI CTYRAG 1 cut(s) 14
Sse9I AATT 7 cut(s) 108, 237, 384, 564, 621, 761, 806
SsiI CCGC 3 cut(s) 119, 449, 866
SspMI CTAG 1 cut(s) 611
SstI GAGCTC 1 cut(s) 15
StyD4I CCNGG 1 cut(s) 23
StyI CCWWGG 1 cut(s) 300
TaaI ACNGT 2 cut(s) 544, 736
TaiI ACGT 1 cut(s) 675
TaqI TCGA 6 cut(s) 5, 34, 203, 314, 481, 755
TasI AATT 7 cut(s) 108, 237, 384, 564, 621, 761, 806
TfiI GAWTC 2 cut(s) 205, 483
Tru1I TTAA 2 cut(s) 30, 765
Tru9I TTAA 2 cut(s) 30, 765
TseFI GTSAC 1 cut(s) 625
TseI GCWGC 1 cut(s) 188
Tsp45I GTSAC 1 cut(s) 625
TspDTI ATGAA 2 cut(s) 346, 689
Van91I CCANNNNNTGG 1 cut(s) 843
VspI ATTAAT 1 cut(s) 30
XapI RAATTY 2 cut(s) 108, 761
XceI RCATGY 1 cut(s) 729
XspI CTAG 1 cut(s) 611
ZraI GACGTC 1 cut(s) 673
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.