Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0003942

Physical Location & Seq

Genomic Coordinates Reverse (-)

61334111 .. 61336261

Gene Structure 2151 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000004676

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1647 bp

ATGGAGTTGGACAGAATAAGCAACTTACCTGGTGGTGTTATACAAAAGATTTTGTCATATTTGCCGATCAGGGAGGCGGTGAGGACATGTGTTTTGTCGAGCAACTGGAGGTGCAAATGGGCTATGCTTCCCCGTCTCGTGTTTGATGATCATCAAATCGGAACAACCAGCAAGGCGAGGAGGAGGAAAACTTCATTTGTGAGCATTGTTGATCGTGTTCTGTTACTTCAAATCGGCCCTGTAGATGCTTTTGACCTTGTTTATCGGAAATTTATAGCCGCTAGTGACGTTGACAGATGGATTCTTCATTTAACTAGAAAATCTTTGAAAGAGTTCACACTCACAAAATCCAGGGGGTCTCAGTACAAGATATCGTCATGTTTGTTTTCTTGCCAAGAGTTGATTCATTTGGATTTAAACAACTGTTCGCTTTGCCCCCCTTCGAGATTCAAAGGCTTCCGGAGATTGAAGAGCCTTCGGCTTCAAAACATTACTGTTGTCCAAGATGTGTTTGAAAAATTGATTGGTTCCTGCCCCCTGCTTGAGAGGCTGACTCTAATTGACTTGAACGGTATCACCCATCTCAAGATCGATGCACCTAATCTCCAATTCCTTGAAGTCCAAGATACTTTTGAGGATGTTAAGGTGGAGAATACCTTAAATCTGGTTGATGTTTTAATTGATTTTGGTGAAGCTTCTAGCTACTGTAGCAATTTGCTTAAGTATTTTGTTCAGCTACCACTTATGGAAAGGCTTACAATCAAGGGTTTCCTTTCAAAGTGTGCTATTGGTACCTCGGCAGAGGAGCTGCCAATACCATGTCGATGTCTGAAGTTCCTTTCTATAGGCATACGCTTGAATAATTTGGAGGAGATATTAGCTGCTATATGCTTTCTGAGAAGCTCACCTGCTCTACAAGAACTCAAAATTTCAGTTCACCAAGAAGGTGAGGCTACTGCTGGAGAAGCGACCTATTGGTTAAATGAAAATCATAATTGGGCATTCACCCAACTGCGACTTATGAAAGTGACTGCCTTCTCTGGTGTTAAAGCTGAAGTAGACTTCATCAAATTTCTTCTTTTGAGCTCTCCTGCTCTTGAAGAACTAGAAATTGTGGCACCTTTTGAAGATCAGGCTGTTTCAGGAGAAGTGAATTCTTGGTTAGATGACAACCTGAACTGGGCATTCACCCAACTGCGACTTGTGAAAGTAACTTGCTTCTCTGGTGTGAAAGCTGAGGTAGACTTCATTAAGTTTCTGCTTTTGAGCTCTCCTGCTCTTCAAGAACTACAAATCTCGGCTCGCCATCATGTTGTTCTGGGAGAGGTGAACCCATGGTCAGATGACAACCAGAATTGTGTATTTACCCACCTACGAGTTGTGCAATTAACAGGCATTCATGGAGTCAAACCTGAAGTAGATTTCATCAGATTTCTGCTTTCAAGTTCACCTGTGCTTGAGAGGATGACTATTCAGCCTACTTCTGTAGATTGTTCTTTGGAGCTGTTAAAGAAATTGGTCCAGTTTAGGCGTGCGTCAGTGGATGCAGAGATAAGCTACTTGGATCCATTGAGTTCAGAATCTGATGAGGATTCTGACTTTGATGATGATTATACTGATCTTGAGTCTGATTCAGATTCTGACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

F-box FBD LRR_2

Protein Analysis

549

Amino Acids

62.06

Weight (kDa)

5.29

Isoelectric Point (pI)

35.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRR_At1g61320_AtMIF1	PF23622	90 - 371	6.6e-19	At1g61320/AtMIF1, LRR domain
LRR_At5g56370	PF24758	97 - 310	4.5e-17	FBD-associated F-box protein At5g56370, LRR repeats
FBD	PF08387	453 - 491	2.5e-07	FBD

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003942)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g25150 FvH4_5g01320 FvH4_5g01330 FvH4_5g09560 FvH4_5g09560 FvH4_5g09560 FvH4_5g09560 FvH4_5g09560 FvH4_5g09560
rosa_chinensis	RchiOBHm_Chr5g0049261 RchiOBHm_Chr7g0188631 RchiOBHm_Chr7g0199601
rosa_laevigata	RLG00000003784 RLG00000004676 RLG00000034607
rosa_multiflora	Rmu_co8228169.1_g000001 Rmu_sc0005358.1_g000008 Rmu_sc0009191.1_g000012 Rmu_sc0018006.1_g000001
rosa_roxburghii	Rroxscaffold_1G00032400 Rroxscaffold_3G00256570 Rroxscaffold_3G00256590 Rroxscaffold_3G00266180
rosa_rugosa	Rorug05G0246700 Rorug06G0491900 Rorug07G0055400
rosa_samantha	Rh5BG334700 Rh5DG346800 Rh7CG099700 Rh7CG189900 Rh7CG190000 Rh7DG098800
rosa_wichuraiana	Rw7G008360 Rw7G015840

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	916
Acc36I	ACCTGC	1 cut(s)	916
Acc65I	GGTACC	1 cut(s)	791
AccB1I	GGYRCC	2 cut(s)	791, 1117
AccI	GTMKAC	2 cut(s)	1059, 1242
AccIII	TCCGGA	1 cut(s)	459
AciI	CCGC	2 cut(s)	77, 279
AclWI	GGATC	2 cut(s)	1559, 1572
AcsI	RAATTY	4 cut(s)	269, 927, 1070, 1153
AcuI	CTGAAG	3 cut(s)	851, 1074, 1434
AfaI	GTAC	2 cut(s)	365, 793
AfiI	CCNNNNNNNGG	1 cut(s)	1180
AflII	CTTAAG	1 cut(s)	719
AflIII	ACRYGT	1 cut(s)	86
AjnI	CCWGG	2 cut(s)	28, 350
AjuI	GAANNNNNNNTTGG	4 cut(s)	387, 419, 507, 539
Alw21I	GWGCWC	2 cut(s)	1088, 1271
Alw26I	GTCTC	2 cut(s)	140, 363
AlwI	GGATC	2 cut(s)	1559, 1572
AlwNI	CAGNNNCTG	2 cut(s)	1583, 1640
Aor13HI	TCCGGA	1 cut(s)	459
AoxI	GGCC	1 cut(s)	235
ApeKI	GCWGC	2 cut(s)	808, 881
ApoI	RAATTY	4 cut(s)	269, 927, 1070, 1153
ArsI	GACNNNNNNTTYG	2 cut(s)	76, 108
Asp700I	GAANNNNTTC	1 cut(s)	332
Asp718I	GGTACC	1 cut(s)	791
AspS9I	GGNCC	2 cut(s)	236, 1519
AvaII	GGWCC	1 cut(s)	1519
BamHI	GGATCC	1 cut(s)	1564
BanI	GGYRCC	2 cut(s)	791, 1117
BanII	GRGCYC	2 cut(s)	1088, 1271
BauI	CACGAG	1 cut(s)	137
Bbv12I	GWGCWC	2 cut(s)	1088, 1271
BbvCI	CCTCAGC	1 cut(s)	1236
BbvI	GCAGC	2 cut(s)	795, 868
BccI	CCATC	3 cut(s)	291, 588, 1314
BciT130I	CCWGG	2 cut(s)	30, 352
BclI	TGATCA	1 cut(s)	148
BcoDI	GTCTC	2 cut(s)	140, 363
BfaI	CTAG	4 cut(s)	282, 315, 699, 1106
BfmI	CTRYAG	4 cut(s)	240, 706, 843, 1485
BfrI	CTTAAG	1 cut(s)	719
BfuAI	ACCTGC	1 cut(s)	916
BisI	GCNGC	3 cut(s)	279, 809, 882
BlsI	GCNGC	3 cut(s)	280, 810, 883
Bme1390I	CCNGG	2 cut(s)	30, 352
Bme18I	GGWCC	1 cut(s)	1519
BmgT120I	GGNCC	2 cut(s)	236, 1519
BmiI	GGNNCC	4 cut(s)	529, 793, 1119, 1566
BmrFI	CCNGG	2 cut(s)	30, 352
BmrI	ACTGGG	1 cut(s)	1189
BmsI	GCATC	3 cut(s)	235, 583, 1534
BmuI	ACTGGG	1 cut(s)	1189
BplI	GAGNNNNNCTC	2 cut(s)	538, 570
BpmI	CTGGAG	2 cut(s)	127, 981
Bpu10I	CCTNAGC	1 cut(s)	1236
BpuEI	CTTGAG	4 cut(s)	563, 569, 1478, 1643
Bsa29I	ATCGAT	1 cut(s)	591
BsaBI	GATNNNNATC	1 cut(s)	150
BsaI	GGTCTC	1 cut(s)	363
BsaJI	CCNNGG	3 cut(s)	351, 795, 1334
BsaWI	WCCGGW	1 cut(s)	459
BsaXI	ACNNNNNCTCC	2 cut(s)	588, 618
Bsc4I	CCNNNNNNNGG	1 cut(s)	1180
Bse1I	ACTGG	3 cut(s)	110, 1184, 1522
Bse8I	GATNNNNATC	1 cut(s)	150
BseAI	TCCGGA	1 cut(s)	459
BseBI	CCWGG	2 cut(s)	30, 352
BseCI	ATCGAT	1 cut(s)	591
BseDI	CCNNGG	3 cut(s)	351, 795, 1334
BseGI	GGATG	3 cut(s)	643, 1470, 1549
BseJI	GATNNNNATC	1 cut(s)	150
BseLI	CCNNNNNNNGG	1 cut(s)	1180
BseMII	CTCAG	3 cut(s)	374, 887, 1227
BseNI	ACTGG	3 cut(s)	110, 1184, 1522
BseRI	GAGGAG	4 cut(s)	193, 196, 818, 884
BseXI	GCAGC	2 cut(s)	795, 868
BshFI	GGCC	1 cut(s)	237
BshNI	GGYRCC	2 cut(s)	791, 1117
BshVI	ATCGAT	1 cut(s)	591
BsiHKAI	GWGCWC	2 cut(s)	1088, 1271
BsiSI	CCGG	1 cut(s)	460
BslI	CCNNNNNNNGG	1 cut(s)	1180
BsmAI	GTCTC	2 cut(s)	140, 363
BsmBI	CGTCTC	1 cut(s)	140
BsmI	GAATGC	3 cut(s)	1001, 1184, 1395
BsnI	GGCC	1 cut(s)	237
Bso31I	GGTCTC	1 cut(s)	363
Bsp1286I	GDGCHC	2 cut(s)	1088, 1271
Bsp13I	TCCGGA	1 cut(s)	459
Bsp143I	GATC	7 cut(s)	66, 148, 211, 588, 1129, 1564, 1618
Bsp19I	CCATGG	1 cut(s)	1334
BspACI	CCGC	2 cut(s)	77, 279
BspANI	GGCC	1 cut(s)	237
BspCNI	CTCAG	3 cut(s)	373, 888, 1228
BspDI	ATCGAT	1 cut(s)	591
BspEI	TCCGGA	1 cut(s)	459
BspLI	GGNNCC	4 cut(s)	529, 793, 1119, 1566
BspMI	ACCTGC	1 cut(s)	916
BspPI	GGATC	2 cut(s)	1559, 1572
BspQI	GCTCTTC	2 cut(s)	464, 1284
BspT107I	GGYRCC	2 cut(s)	791, 1117
BspTI	CTTAAG	1 cut(s)	719
BspTNI	GGTCTC	1 cut(s)	363
BsrI	ACTGG	3 cut(s)	110, 1184, 1522
BssECI	CCNNGG	3 cut(s)	351, 795, 1334
BssMI	GATC	7 cut(s)	66, 148, 211, 588, 1129, 1564, 1618
BssSI	CACGAG	1 cut(s)	137
BssT1I	CCWWGG	1 cut(s)	1334
Bst2BI	CACGAG	1 cut(s)	137
Bst2UI	CCWGG	2 cut(s)	30, 352
Bst4CI	ACNGT	4 cut(s)	425, 496, 572, 707
Bst6I	CTCTTC	2 cut(s)	464, 1284
BstAFI	CTTAAG	1 cut(s)	719
BstC8I	GCNNGC	2 cut(s)	1303, 1533
BstDEI	CTNAG	3 cut(s)	360, 896, 1236
BstDSI	CCRYGG	1 cut(s)	1334
BstF5I	GGATG	3 cut(s)	643, 1470, 1549
BstKTI	GATC	7 cut(s)	69, 151, 214, 591, 1132, 1567, 1621
BstMAI	GTCTC	2 cut(s)	140, 363
BstMBI	GATC	7 cut(s)	66, 148, 211, 588, 1129, 1564, 1618
BstMWI	GCNNNNNNNGC	3 cut(s)	547, 708, 965
BstNI	CCWGG	2 cut(s)	30, 352
BstNSI	RCATGY	1 cut(s)	90
BstSCI	CCNGG	2 cut(s)	28, 350
BstSFI	CTRYAG	4 cut(s)	240, 706, 843, 1485
BstV1I	GCAGC	2 cut(s)	795, 868
BstX2I	RGATCY	1 cut(s)	1564
BstYI	RGATCY	1 cut(s)	1564
Bsu15I	ATCGAT	1 cut(s)	591
BsuRI	GGCC	1 cut(s)	237
BsuTUI	ATCGAT	1 cut(s)	591
BtgI	CCRYGG	1 cut(s)	1334
BtsCI	GGATG	3 cut(s)	643, 1470, 1549
BtsIMutI	CAGTG	1 cut(s)	1545
BveI	ACCTGC	1 cut(s)	916
Cac8I	GCNNGC	2 cut(s)	1303, 1533
CaiI	CAGNNNCTG	2 cut(s)	1583, 1640
Cfr13I	GGNCC	2 cut(s)	236, 1519
ClaI	ATCGAT	1 cut(s)	591
CseI	GACGC	1 cut(s)	1524
CsiI	ACCWGGT	1 cut(s)	28
Csp6I	GTAC	2 cut(s)	364, 792
CviAII	CATG	6 cut(s)	87, 378, 819, 1310, 1335, 1400
CviQI	GTAC	2 cut(s)	364, 792
DdeI	CTNAG	3 cut(s)	360, 896, 1236
DpnI	GATC	7 cut(s)	68, 150, 213, 590, 1131, 1566, 1620
DpnII	GATC	7 cut(s)	66, 148, 211, 588, 1129, 1564, 1618
DraI	TTTAAA	1 cut(s)	417
Eam1104I	CTCTTC	2 cut(s)	464, 1284
EarI	CTCTTC	2 cut(s)	464, 1284
Ecl136II	GAGCTC	2 cut(s)	1086, 1269
Eco130I	CCWWGG	1 cut(s)	1334
Eco24I	GRGCYC	2 cut(s)	1088, 1271
Eco31I	GGTCTC	1 cut(s)	363
Eco32I	GATATC	1 cut(s)	372
Eco47I	GGWCC	1 cut(s)	1519
Eco53kI	GAGCTC	2 cut(s)	1086, 1269
Eco57I	CTGAAG	3 cut(s)	851, 1074, 1434
EcoICRI	GAGCTC	2 cut(s)	1086, 1269
EcoRI	GAATTC	1 cut(s)	1153
EcoRII	CCWGG	2 cut(s)	28, 350
EcoRV	GATATC	1 cut(s)	372
EcoT14I	CCWWGG	1 cut(s)	1334
EcoT38I	GRGCYC	2 cut(s)	1088, 1271
ErhI	CCWWGG	1 cut(s)	1334
Esp3I	CGTCTC	1 cut(s)	140
FaeI	CATG	6 cut(s)	90, 381, 822, 1313, 1338, 1403
FatI	CATG	6 cut(s)	86, 377, 818, 1309, 1334, 1399
FbaI	TGATCA	1 cut(s)	148
FblI	GTMKAC	2 cut(s)	1059, 1242
Fnu4HI	GCNGC	3 cut(s)	279, 809, 882
FokI	GGATG	3 cut(s)	650, 1477, 1556
FriOI	GRGCYC	2 cut(s)	1088, 1271
Fsp4HI	GCNGC	3 cut(s)	279, 809, 882
FspBI	CTAG	4 cut(s)	282, 315, 699, 1106
GluI	GCNGC	3 cut(s)	279, 809, 882
GsuI	CTGGAG	2 cut(s)	127, 981
HaeIII	GGCC	1 cut(s)	237
HapII	CCGG	1 cut(s)	460
HgaI	GACGC	1 cut(s)	1524
Hin1II	CATG	6 cut(s)	90, 381, 822, 1313, 1338, 1403
HincII	GTYRAC	1 cut(s)	292
HindII	GTYRAC	1 cut(s)	292
HindIII	AAGCTT	1 cut(s)	693
HpaII	CCGG	1 cut(s)	460
Hpy166II	GTNNAC	7 cut(s)	292, 336, 937, 1060, 1243, 1330, 1448
Hpy188III	TCNNGA	7 cut(s)	444, 460, 586, 1097, 1143, 1283, 1622
Hpy8I	GTNNAC	7 cut(s)	292, 336, 937, 1060, 1243, 1330, 1448
HpyAV	CCTTC	4 cut(s)	450, 485, 938, 1045
HpyCH4III	ACNGT	4 cut(s)	425, 496, 572, 707
HpyCH4IV	ACGT	1 cut(s)	288
HpyCH4V	TGCA	4 cut(s)	114, 596, 1384, 1547
HpyF10VI	GCNNNNNNNGC	3 cut(s)	547, 708, 965
HpyF3I	CTNAG	3 cut(s)	360, 896, 1236
HpySE526I	ACGT	1 cut(s)	288
Hsp92II	CATG	6 cut(s)	90, 381, 822, 1313, 1338, 1403
Kpn2I	TCCGGA	1 cut(s)	459
KpnI	GGTACC	1 cut(s)	795
Ksp22I	TGATCA	1 cut(s)	148
Kzo9I	GATC	7 cut(s)	66, 148, 211, 588, 1129, 1564, 1618
LguI	GCTCTTC	2 cut(s)	464, 1284
LmnI	GCTCC	2 cut(s)	805, 1501
Lsp1109I	GCAGC	2 cut(s)	795, 868
LweI	GCATC	3 cut(s)	235, 583, 1534
MabI	ACCWGGT	1 cut(s)	28
MaeI	CTAG	4 cut(s)	282, 315, 699, 1106
MaeII	ACGT	1 cut(s)	288
MaeIII	GTNAC	4 cut(s)	222, 284, 1027, 1210
MalI	GATC	7 cut(s)	68, 150, 213, 590, 1131, 1566, 1620
MboI	GATC	7 cut(s)	66, 148, 211, 588, 1129, 1564, 1618
MboII	GAAGA	6 cut(s)	296, 481, 1067, 1112, 1139, 1271
MflI	RGATCY	1 cut(s)	1564
MhlI	GDGCHC	2 cut(s)	1088, 1271
MlyI	GAGTC	3 cut(s)	547, 1413, 1634
MroI	TCCGGA	1 cut(s)	459
MroXI	GAANNNNTTC	1 cut(s)	332
MslI	CAYNNNNRTG	1 cut(s)	823
MspCI	CTTAAG	1 cut(s)	719
MspI	CCGG	1 cut(s)	460
MspR9I	CCNGG	2 cut(s)	30, 352
Mva1269I	GAATGC	3 cut(s)	1001, 1184, 1395
MvaI	CCWGG	2 cut(s)	30, 352
MwoI	GCNNNNNNNGC	3 cut(s)	547, 708, 965
NcoI	CCATGG	1 cut(s)	1334
NdeII	GATC	7 cut(s)	66, 148, 211, 588, 1129, 1564, 1618
NlaIII	CATG	6 cut(s)	90, 381, 822, 1313, 1338, 1403
NlaIV	GGNNCC	4 cut(s)	529, 793, 1119, 1566
NmeAIII	GCCGAG	2 cut(s)	776, 1277
NmuCI	GTSAC	2 cut(s)	284, 1027
NspI	RCATGY	1 cut(s)	90
PaqCI	CACCTGC	1 cut(s)	916
PciI	ACATGT	1 cut(s)	86
PciSI	GCTCTTC	2 cut(s)	464, 1284
PctI	GAATGC	3 cut(s)	1001, 1184, 1395
PdmI	GAANNNNTTC	1 cut(s)	332
PfeI	GAWTC	7 cut(s)	301, 403, 447, 1580, 1592, 1631, 1637
PkrI	GCNGC	3 cut(s)	280, 810, 883
PleI	GAGTC	3 cut(s)	547, 1412, 1633
PpsI	GAGTC	3 cut(s)	547, 1412, 1633
PscI	ACATGT	1 cut(s)	86
Psp124BI	GAGCTC	2 cut(s)	1088, 1271
Psp6I	CCWGG	2 cut(s)	28, 350
PspGI	CCWGG	2 cut(s)	28, 350
PspN4I	GGNNCC	4 cut(s)	529, 793, 1119, 1566
PspPI	GGNCC	2 cut(s)	236, 1519
PstNI	CAGNNNCTG	2 cut(s)	1583, 1640
PsuI	RGATCY	1 cut(s)	1564
RsaI	GTAC	2 cut(s)	365, 793
RsaNI	GTAC	2 cut(s)	364, 792
RseI	CAYNNNNRTG	1 cut(s)	823
SacI	GAGCTC	2 cut(s)	1088, 1271
SapI	GCTCTTC	2 cut(s)	464, 1284
SatI	GCNGC	3 cut(s)	279, 809, 882
Sau3AI	GATC	7 cut(s)	66, 148, 211, 588, 1129, 1564, 1618
Sau96I	GGNCC	2 cut(s)	236, 1519
SchI	GAGTC	3 cut(s)	547, 1413, 1634
ScrFI	CCNGG	2 cut(s)	30, 352
SduI	GDGCHC	2 cut(s)	1088, 1271
SexAI	ACCWGGT	1 cut(s)	28
SfaNI	GCATC	3 cut(s)	235, 583, 1534
SfcI	CTRYAG	4 cut(s)	240, 706, 843, 1485
SinI	GGWCC	1 cut(s)	1519
SmiMI	CAYNNNNRTG	1 cut(s)	823
SmlI	CTYRAG	5 cut(s)	542, 584, 719, 1457, 1622
SmoI	CTYRAG	5 cut(s)	542, 584, 719, 1457, 1622
SsiI	CCGC	2 cut(s)	77, 279
SspMI	CTAG	4 cut(s)	282, 315, 699, 1106
SstI	GAGCTC	2 cut(s)	1088, 1271
StyD4I	CCNGG	2 cut(s)	28, 350
StyI	CCWWGG	1 cut(s)	1334
TaaI	ACNGT	4 cut(s)	425, 496, 572, 707
TaiI	ACGT	1 cut(s)	291
TaqI	TCGA	4 cut(s)	98, 443, 591, 823
TatI	WGTACW	1 cut(s)	363
TauI	GCSGC	1 cut(s)	281
TfiI	GAWTC	7 cut(s)	301, 403, 447, 1580, 1592, 1631, 1637
TscAI	CASTG	1 cut(s)	1545
TseFI	GTSAC	2 cut(s)	284, 1027
TseI	GCWGC	2 cut(s)	808, 881
Tsp45I	GTSAC	2 cut(s)	284, 1027
TspDTI	ATGAA	9 cut(s)	183, 296, 395, 999, 1037, 1054, 1237, 1388, 1414
TspRI	CASTG	1 cut(s)	1545
Vha464I	CTTAAG	1 cut(s)	719
VpaK11BI	GGWCC	1 cut(s)	1519
XapI	RAATTY	4 cut(s)	269, 927, 1070, 1153
XceI	RCATGY	1 cut(s)	90
XmiI	GTMKAC	2 cut(s)	1059, 1242
XmnI	GAANNNNTTC	1 cut(s)	332
XspI	CTAG	4 cut(s)	282, 315, 699, 1106

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.