Rroxscaffold_1G00032400

F-box FBD LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0003942
Physical Location & Seq
Forward (+)
46351009 .. 46353849
2841 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00032400.1

Sequence Viewer

Length: 1761 bp
ATGGAGTTGGACAGAATAAGCAACTTACCAAGTAATGTTGCACAACAAATTTTGTCATATTTGCCGATCAAGGATGCAGGGAAGACTAGTATTTTATCAAAAGACTGGAGGTTCAAATGGACTATGCTTCCTTCTCTTGTTTTTGATCATGAAATAGATGACCCGGACAGTGTGGATCACGTTCTCTTACTTCACCCTGGTCCCATATACTCTTTCAAGCTTTCTGCACAAAACATTATGGACAAAAGTGCTATTAACCGATGGATTCGTCATCTATCAAGACAACCTATCAAACAGTTCATACTGAAAGGGTATTATAATTTTTATAGTGATAGGCGACCTTACGATGTATCTTCCTCTTTGTTTTCTTGTCAAGATTTGACTCATTTAGAGTTAAGCGATTGTTCGCTAAGACTCCCGTGGACTTTCAATGGCTTCAGGATGTTGAAGACCCTTAATATTAAGAGAGTTACTGTCGCCCGAGATGAATTGGAAAAGATGATTGTTTGTAGTCCTTTGCTTGAGAGATTAACTCTACGTGACTTACAGGGTATCACCCGTCTCAAGATTCACGCACCGAATCTCCAATTCCTTAAAGTTCGAGGAGATTTTGAAGATGTTGAACTTACGAATACCTTAAATCTTGTTCATGTTTCACTTGACCGAAATAGGGCTCATGGGAGAGGGGACAGATTGCTCAACTCGTGTGTTAAGTTGCCTTATATCAAAAGGCTCACAATTGAGGGTTGCATTTTGGAGTGTGCTTCTGGTTCCTTGCCGAAGGAGCTGCGTAGACCATGTTCATATCTGAACTTTCTTTCTATAAACATACGCTTGAATAACTTGGAGGAGATTTCAACTGCCATGTGCCTTCTCAGAAGCTCACCTGCTCTGCAGGAACTAGAAGTTTCAGTTCGCCAACAAAATGAGGAGGCTAGTACTGCTGGAGGAGCAACTCCTTGGTTAAATGACAGCCAGAATTGGGAATTCACCAAACTGCAACGTGTAAAACTGACCGGCTTCTCTGGTGCCCAAGCTGAAGTAGACTTCATCAAATTTCTGATTTTAAGCTGCCCTGCTCTCCAAGAACTAGAAGTTTCAGTTTACCAAAAAGACAAGACAGTTGTAGGAGAAGTGGACTATTCTTGGTTAAATAACGACCAAAATTTTGCACACACCCAACTGCGACTTTTGAAAGTAACCGACTTTTGTGGTGAAGTAGAATTCATCAAGTTTCTGCTTTTAAGCTCCCCTGCTCTTCAAGAACTACATATTTTGTTTAGAGAGAATGATCATAAGAACAAGATGAGAAACAAGTGGTTAGCTATGGGAGATGAGGACAACCGGAATTGCACATTCACCCAACTTCGAGTTATGAACATTAGTGGCTTTTCTGGTGCCAAAGCTGAAGTAGGTTTCATCAAATTTTTGCTTACAAGTTCACCTATGCTTGAGAGGATAACTCTTCAACCTGCTTCTGCCAAAGTTCCTTGGGAAGTAACATCTTCTTCTCCAGAGTCCAGACCGCCTTCGTCTTCCTCCGGCGCCAAAGATCCCAGACCGCCGTCGCTCCTCGTCGTCGACCGGATTCTTCAGTTCGCAGCCCAGACGATCTACAGTTCATCGCTCTCCAAAGATCGACACCAGATTTTGCGTCGTCATCAGTCCGGTTCTCTCCGAGAGCGGCGGCGTCATCCTTCATCCATCCCAGATCGGATTCGCTGTTTTTCCCAGACCGTCATAGCTACAGATCGTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD LRR_2

Protein Analysis

586

Amino Acids

67.05

Weight (kDa)

8.94

Isoelectric Point (pI)

53.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 6 - 45 4.9e-07 F-box domain
LRR_At1g61320_AtMIF1 PF23622 78 - 200 3e-12 At1g61320/AtMIF1, LRR domain
LRR_At5g56370 PF24758 86 - 303 7.5e-16 FBD-associated F-box protein At5g56370, LRR repeats
LRR_At1g61320_AtMIF1 PF23622 191 - 374 3.4e-08 At1g61320/AtMIF1, LRR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 318
AarI CACCTGC 1 cut(s) 895
Acc36I ACCTGC 2 cut(s) 895, 1480
AccB1I GGYRCC 3 cut(s) 1028, 1397, 1544
AccBSI CCGCTC 1 cut(s) 1684
AccI GTMKAC 3 cut(s) 793, 1044, 1581
AciI CCGC 4 cut(s) 1526, 1562, 1684, 1687
AclWI GGATC 2 cut(s) 183, 1547
AcsI RAATTY 6 cut(s) 48, 986, 1055, 1165, 1223, 1424
AcuI CTGAAG 4 cut(s) 421, 1059, 1428, 1577
AcyI GRCGYC 2 cut(s) 1545, 1690
AfaI GTAC 1 cut(s) 940
AfiI CCNNNNNNNGG 2 cut(s) 670, 982
AflIII ACRYGT 1 cut(s) 1003
AhlI ACTAGT 1 cut(s) 86
AjnI CCWGG 1 cut(s) 196
AluBI AGCT 9 cut(s) 220, 787, 882, 1037, 1071, 1248, 1325, 1406, 1745
AluI AGCT 9 cut(s) 220, 787, 882, 1037, 1071, 1248, 1325, 1406, 1745
Alw26I GTCTC 1 cut(s) 566
AlwI GGATC 2 cut(s) 183, 1547
Ama87I CYCGRG 1 cut(s) 480
ApeKI GCWGC 3 cut(s) 787, 1071, 1601
ApoI RAATTY 6 cut(s) 48, 986, 1055, 1165, 1223, 1424
ArsI GACNNNNNNTTYG 2 cut(s) 1151, 1183
AspLEI GCGC 1 cut(s) 1547
AspS9I GGNCC 1 cut(s) 200
AsuC2I CCSGG 1 cut(s) 164
AsuHPI GGTGA 7 cut(s) 185, 547, 876, 982, 1226, 1351, 1434
AvaI CYCGRG 1 cut(s) 480
AvaII GGWCC 1 cut(s) 200
BaeGI GKGCMC 1 cut(s) 1033
BanI GGYRCC 3 cut(s) 1028, 1397, 1544
BanII GRGCYC 1 cut(s) 676
BauI CACGAG 1 cut(s) 703
BbsI GAAGAC 3 cut(s) 89, 455, 1527
BbvI GCAGC 3 cut(s) 774, 1058, 1613
BccI CCATC 2 cut(s) 255, 1712
BceAI ACGGC 1 cut(s) 1549
BcgI CGANNNNNNTGC 2 cut(s) 769, 803
BciT130I CCWGG 1 cut(s) 198
BclI TGATCA 2 cut(s) 145, 1291
BcnI CCSGG 1 cut(s) 164
BcoDI GTCTC 1 cut(s) 566
BcuI ACTAGT 1 cut(s) 86
BfaI CTAG 4 cut(s) 87, 902, 936, 1091
BfmI CTRYAG 3 cut(s) 893, 1615, 1746
BfoI RGCGCY 1 cut(s) 1548
BfuAI ACCTGC 2 cut(s) 895, 1480
BisI GCNGC 5 cut(s) 788, 1072, 1602, 1685, 1688
BlsI GCNGC 5 cut(s) 789, 1073, 1603, 1686, 1689
BmcAI AGTACT 1 cut(s) 940
Bme1390I CCNGG 2 cut(s) 164, 198
Bme18I GGWCC 1 cut(s) 200
BmeT110I CYCGRG 1 cut(s) 480
BmgT120I GGNCC 1 cut(s) 200
BmiI GGNNCC 5 cut(s) 202, 772, 1030, 1399, 1546
BmrFI CCNGG 2 cut(s) 164, 198
BmsI GCATC 1 cut(s) 64
BoxI GACNNNNGTC 1 cut(s) 1564
BpiI GAAGAC 3 cut(s) 89, 455, 1527
BplI GAGNNNNNCTC 4 cut(s) 517, 549, 1447, 1479
BpmI CTGGAG 3 cut(s) 127, 966, 1497
BpuEI CTTGAG 3 cut(s) 542, 548, 1472
BpuMI CCSGG 1 cut(s) 164
BsaAI YACGTR 1 cut(s) 539
BsaHI GRCGYC 2 cut(s) 1545, 1690
BsaJI CCNNGG 4 cut(s) 196, 419, 959, 1490
BsaWI WCCGGW 3 cut(s) 1344, 1584, 1667
BsaXI ACNNNNNCTCC 2 cut(s) 567, 597
Bsc4I CCNNNNNNNGG 2 cut(s) 670, 982
Bse118I RCCGGY 1 cut(s) 1016
Bse1I ACTGG 1 cut(s) 110
BseBI CCWGG 1 cut(s) 198
BseDI CCNNGG 4 cut(s) 196, 419, 959, 1490
BseGI GGATG 5 cut(s) 79, 447, 1693, 1700, 1704
BseLI CCNNNNNNNGG 2 cut(s) 670, 982
BseMII CTCAG 1 cut(s) 889
BseNI ACTGG 1 cut(s) 110
BseRI GAGGAG 5 cut(s) 618, 863, 944, 963, 1562
BseSI GKGCMC 1 cut(s) 1033
BseXI GCAGC 3 cut(s) 774, 1058, 1613
BsgI GTGCAG 1 cut(s) 210
Bsh1285I CGRYCG 1 cut(s) 1585
BshNI GGYRCC 3 cut(s) 1028, 1397, 1544
BsiEI CGRYCG 1 cut(s) 1585
BsiHKCI CYCGRG 1 cut(s) 480
BsiSI CCGG 6 cut(s) 164, 1017, 1345, 1542, 1585, 1668
BslFI GGGAC 2 cut(s) 186, 701
BslI CCNNNNNNNGG 2 cut(s) 670, 982
BsmAI GTCTC 1 cut(s) 566
BsmBI CGTCTC 1 cut(s) 566
BsmFI GGGAC 2 cut(s) 186, 701
BsoBI CYCGRG 1 cut(s) 480
Bsp1286I GDGCHC 2 cut(s) 676, 1033
Bsp143I GATC 9 cut(s) 66, 145, 175, 1291, 1552, 1611, 1636, 1711, 1750
BspACI CCGC 4 cut(s) 1526, 1562, 1684, 1687
BspCNI CTCAG 1 cut(s) 888
BspHI TCATGA 1 cut(s) 148
BspLI GGNNCC 5 cut(s) 202, 772, 1030, 1399, 1546
BspMAI CTGCAG 1 cut(s) 897
BspMI ACCTGC 2 cut(s) 895, 1480
BspPI GGATC 2 cut(s) 183, 1547
BspQI GCTCTTC 1 cut(s) 1263
BspT107I GGYRCC 3 cut(s) 1028, 1397, 1544
BsrBI CCGCTC 1 cut(s) 1684
BsrFI RCCGGY 1 cut(s) 1016
BsrI ACTGG 1 cut(s) 110
BssAI RCCGGY 1 cut(s) 1016
BssECI CCNNGG 4 cut(s) 196, 419, 959, 1490
BssMI GATC 9 cut(s) 66, 145, 175, 1291, 1552, 1611, 1636, 1711, 1750
BssNI GRCGYC 2 cut(s) 1545, 1690
BssSI CACGAG 1 cut(s) 703
BssT1I CCWWGG 2 cut(s) 959, 1490
Bst2BI CACGAG 1 cut(s) 703
Bst2UI CCWGG 1 cut(s) 198
Bst4CI ACNGT 6 cut(s) 170, 297, 475, 1123, 1619, 1738
Bst6I CTCTTC 2 cut(s) 1263, 1470
BstACI GRCGYC 2 cut(s) 1545, 1690
BstBAI YACGTR 1 cut(s) 539
BstDEI CTNAG 2 cut(s) 410, 875
BstDSI CCRYGG 1 cut(s) 419
BstF5I GGATG 5 cut(s) 79, 447, 1693, 1700, 1704
BstH2I RGCGCY 1 cut(s) 1548
BstHHI GCGC 1 cut(s) 1547
BstKTI GATC 9 cut(s) 69, 148, 178, 1294, 1555, 1614, 1639, 1714, 1753
BstMAI GTCTC 1 cut(s) 566
BstMBI GATC 9 cut(s) 66, 145, 175, 1291, 1552, 1611, 1636, 1711, 1750
BstMCI CGRYCG 1 cut(s) 1585
BstMWI GCNNNNNNNGC 3 cut(s) 784, 941, 950
BstNI CCWGG 1 cut(s) 198
BstPAI GACNNNNGTC 1 cut(s) 1564
BstSCI CCNGG 2 cut(s) 162, 196
BstSFI CTRYAG 3 cut(s) 893, 1615, 1746
BstSLI GKGCMC 1 cut(s) 1033
BstV1I GCAGC 3 cut(s) 774, 1058, 1613
BstV2I GAAGAC 3 cut(s) 89, 455, 1527
BstX2I RGATCY 1 cut(s) 1552
BstYI RGATCY 1 cut(s) 1552
BtgI CCRYGG 1 cut(s) 419
BtgZI GCGATG 1 cut(s) 1608
BtsCI GGATG 5 cut(s) 79, 447, 1693, 1700, 1704
BtsIMutI CAGTG 1 cut(s) 175
BveI ACCTGC 2 cut(s) 895, 1480
CciI TCATGA 1 cut(s) 148
CfoI GCGC 1 cut(s) 1547
Cfr10I RCCGGY 1 cut(s) 1016
Cfr13I GGNCC 1 cut(s) 200
CseI GACGC 2 cut(s) 1643, 1679
Csp6I GTAC 1 cut(s) 939
CviAII CATG 5 cut(s) 149, 650, 677, 798, 865
CviQI GTAC 1 cut(s) 939
DdeI CTNAG 2 cut(s) 410, 875
DinI GGCGCC 1 cut(s) 1546
DpnI GATC 9 cut(s) 68, 147, 177, 1293, 1554, 1613, 1638, 1713, 1752
DpnII GATC 9 cut(s) 66, 145, 175, 1291, 1552, 1611, 1636, 1711, 1750
Eam1104I CTCTTC 2 cut(s) 1263, 1470
EarI CTCTTC 2 cut(s) 1263, 1470
Eco130I CCWWGG 2 cut(s) 959, 1490
Eco24I GRGCYC 1 cut(s) 676
Eco47I GGWCC 1 cut(s) 200
Eco57I CTGAAG 4 cut(s) 421, 1059, 1428, 1577
Eco88I CYCGRG 1 cut(s) 480
EcoRI GAATTC 2 cut(s) 986, 1223
EcoRII CCWGG 1 cut(s) 196
EcoT14I CCWWGG 2 cut(s) 959, 1490
EcoT38I GRGCYC 1 cut(s) 676
EgeI GGCGCC 1 cut(s) 1546
EheI GGCGCC 1 cut(s) 1546
ErhI CCWWGG 2 cut(s) 959, 1490
Esp3I CGTCTC 1 cut(s) 566
FaeI CATG 5 cut(s) 152, 653, 680, 801, 868
FaqI GGGAC 2 cut(s) 186, 701
FatI CATG 5 cut(s) 148, 649, 676, 797, 864
FbaI TGATCA 2 cut(s) 145, 1291
FblI GTMKAC 3 cut(s) 793, 1044, 1581
Fnu4HI GCNGC 5 cut(s) 788, 1072, 1602, 1685, 1688
FokI GGATG 5 cut(s) 86, 454, 1680, 1687, 1691
FriOI GRGCYC 1 cut(s) 676
Fsp4HI GCNGC 5 cut(s) 788, 1072, 1602, 1685, 1688
FspBI CTAG 4 cut(s) 87, 902, 936, 1091
GlaI GCGC 1 cut(s) 1546
GluI GCNGC 5 cut(s) 788, 1072, 1602, 1685, 1688
GsuI CTGGAG 3 cut(s) 127, 966, 1497
HaeII RGCGCY 1 cut(s) 1548
HapII CCGG 6 cut(s) 164, 1017, 1345, 1542, 1585, 1668
HgaI GACGC 2 cut(s) 1643, 1679
HhaI GCGC 1 cut(s) 1547
Hin1I GRCGYC 2 cut(s) 1545, 1690
Hin1II CATG 5 cut(s) 152, 653, 680, 801, 868
Hin6I GCGC 1 cut(s) 1545
HinP1I GCGC 1 cut(s) 1545
HincII GTYRAC 1 cut(s) 1582
HindII GTYRAC 1 cut(s) 1582
HindIII AAGCTT 1 cut(s) 218
HinfI GANTC 8 cut(s) 265, 382, 414, 568, 580, 1517, 1588, 1717
HpaII CCGG 6 cut(s) 164, 1017, 1345, 1542, 1585, 1668
HphI GGTGA 7 cut(s) 185, 547, 876, 982, 1226, 1351, 1434
Hpy166II GTNNAC 7 cut(s) 423, 794, 1045, 1105, 1138, 1442, 1582
Hpy188I TCNGA 5 cut(s) 810, 878, 1062, 1679, 1716
Hpy188III TCNNGA 9 cut(s) 149, 279, 374, 439, 565, 1262, 1514, 1521, 1754
Hpy8I GTNNAC 7 cut(s) 423, 794, 1045, 1105, 1138, 1442, 1582
Hpy99I CGWCG 4 cut(s) 1570, 1580, 1583, 1659
HpyAV CCTTC 5 cut(s) 141, 775, 881, 1539, 1707
HpyCH4III ACNGT 6 cut(s) 170, 297, 475, 1123, 1619, 1738
HpyCH4IV ACGT 3 cut(s) 180, 538, 1003
HpyCH4V TGCA 8 cut(s) 41, 77, 227, 750, 895, 1000, 1172, 1353
HpyF10VI GCNNNNNNNGC 3 cut(s) 784, 941, 950
HpyF3I CTNAG 2 cut(s) 410, 875
HpySE526I ACGT 3 cut(s) 180, 538, 1003
Hsp92I GRCGYC 2 cut(s) 1545, 1690
Hsp92II CATG 5 cut(s) 152, 653, 680, 801, 868
HspAI GCGC 1 cut(s) 1545
KasI GGCGCC 1 cut(s) 1544
Ksp22I TGATCA 2 cut(s) 145, 1291
Kzo9I GATC 9 cut(s) 66, 145, 175, 1291, 1552, 1611, 1636, 1711, 1750
LguI GCTCTTC 1 cut(s) 1263
LmnI GCTCC 4 cut(s) 784, 950, 1253, 1575
Lsp1109I GCAGC 3 cut(s) 774, 1058, 1613
LweI GCATC 1 cut(s) 64
MaeI CTAG 4 cut(s) 87, 902, 936, 1091
MaeII ACGT 3 cut(s) 180, 538, 1003
MaeIII GTNAC 5 cut(s) 469, 539, 1198, 1498, 1754
MalI GATC 9 cut(s) 68, 147, 177, 1293, 1554, 1613, 1638, 1713, 1752
MbiI CCGCTC 1 cut(s) 1684
MboI GATC 9 cut(s) 66, 145, 175, 1291, 1552, 1611, 1636, 1711, 1750
MfeI CAATTG 1 cut(s) 738
MflI RGATCY 1 cut(s) 1552
MhlI GDGCHC 2 cut(s) 676, 1033
Mly113I GGCGCC 1 cut(s) 1545
MlyI GAGTC 3 cut(s) 376, 408, 1526
MspI CCGG 6 cut(s) 164, 1017, 1345, 1542, 1585, 1668
MspR9I CCNGG 2 cut(s) 164, 198
MunI CAATTG 1 cut(s) 738
MvaI CCWGG 1 cut(s) 198
MwoI GCNNNNNNNGC 3 cut(s) 784, 941, 950
NarI GGCGCC 1 cut(s) 1545
NciI CCSGG 1 cut(s) 164
NdeII GATC 9 cut(s) 66, 145, 175, 1291, 1552, 1611, 1636, 1711, 1750
NlaIII CATG 5 cut(s) 152, 653, 680, 801, 868
NlaIV GGNNCC 5 cut(s) 202, 772, 1030, 1399, 1546
NmuCI GTSAC 2 cut(s) 539, 1754
PagI TCATGA 1 cut(s) 148
PaqCI CACCTGC 1 cut(s) 895
PciSI GCTCTTC 1 cut(s) 1263
PfeI GAWTC 5 cut(s) 265, 568, 580, 1588, 1717
PkrI GCNGC 5 cut(s) 789, 1073, 1603, 1686, 1689
PleI GAGTC 3 cut(s) 376, 408, 1525
PluTI GGCGCC 1 cut(s) 1548
PpsI GAGTC 3 cut(s) 376, 408, 1525
Ppu21I YACGTR 1 cut(s) 539
PshAI GACNNNNGTC 1 cut(s) 1564
PsiI TTATAA 1 cut(s) 318
Psp6I CCWGG 1 cut(s) 196
PspGI CCWGG 1 cut(s) 196
PspN4I GGNNCC 5 cut(s) 202, 772, 1030, 1399, 1546
PspPI GGNCC 1 cut(s) 200
PsrI GAACNNNNNNTAC 2 cut(s) 784, 816
PstI CTGCAG 1 cut(s) 897
PsuI RGATCY 1 cut(s) 1552
RsaI GTAC 1 cut(s) 940
RsaNI GTAC 1 cut(s) 939
SalI GTCGAC 1 cut(s) 1580
SapI GCTCTTC 1 cut(s) 1263
SatI GCNGC 5 cut(s) 788, 1072, 1602, 1685, 1688
Sau3AI GATC 9 cut(s) 66, 145, 175, 1291, 1552, 1611, 1636, 1711, 1750
Sau96I GGNCC 1 cut(s) 200
ScaI AGTACT 1 cut(s) 940
SchI GAGTC 3 cut(s) 376, 408, 1526
ScrFI CCNGG 2 cut(s) 164, 198
SduI GDGCHC 2 cut(s) 676, 1033
SfaNI GCATC 1 cut(s) 64
SfcI CTRYAG 3 cut(s) 893, 1615, 1746
SfoI GGCGCC 1 cut(s) 1546
SinI GGWCC 1 cut(s) 200
SmlI CTYRAG 3 cut(s) 521, 563, 1451
SmoI CTYRAG 3 cut(s) 521, 563, 1451
SpeI ACTAGT 1 cut(s) 86
SsiI CCGC 4 cut(s) 1526, 1562, 1684, 1687
SspDI GGCGCC 1 cut(s) 1544
SspI AATATT 1 cut(s) 460
SspMI CTAG 4 cut(s) 87, 902, 936, 1091
StyD4I CCNGG 2 cut(s) 162, 196
StyI CCWWGG 2 cut(s) 959, 1490
TaaI ACNGT 6 cut(s) 170, 297, 475, 1123, 1619, 1738
TaiI ACGT 3 cut(s) 183, 541, 1006
TaqI TCGA 4 cut(s) 601, 1369, 1581, 1639
TaqII GACCGA 1 cut(s) 678
TatI WGTACW 1 cut(s) 938
TauI GCSGC 2 cut(s) 1687, 1690
TfiI GAWTC 5 cut(s) 265, 568, 580, 1588, 1717
TscAI CASTG 1 cut(s) 175
TseFI GTSAC 2 cut(s) 539, 1754
TseI GCWGC 3 cut(s) 787, 1071, 1601
Tsp45I GTSAC 2 cut(s) 539, 1754
TspRI CASTG 1 cut(s) 175
VpaK11BI GGWCC 1 cut(s) 200
XapI RAATTY 6 cut(s) 48, 986, 1055, 1165, 1223, 1424
XmiI GTMKAC 3 cut(s) 793, 1044, 1581
XspI CTAG 4 cut(s) 87, 902, 936, 1091
ZrmI AGTACT 1 cut(s) 940
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.