Rh5DG346800

F-box FBD LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0003942
Physical Location & Seq
Reverse (-)
47391404 .. 47393165
1762 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG346800.1

Sequence Viewer

Length: 1704 bp
ATGTCAAGAAGACCACACAATTCCTCCTCTCCGGTAAAAAAACGAAGGAAGATGGAGTTGGACAGAATAAGCAACTTACCAAGTAATGTTACACAACAAATTTTGTCATATTTGCCGATCAAGGATGCAGGGAAGACAAGTATGTTATCAAAAGACTGGAGGTTCAAATGGGCTATGCTTCCTTCTCTTGTTTTTGATCATGAAATAGATGACGTGGACAGTGTGGATCACGTTCTCTTACTTCACCCTGGTCCCATATTCTCTTTCAAGCTTTCTGCACAAAACATTATGGACAAAAGTGCTATTAACCGATGGATTCGTCATCTATCAAGACAACCTATCAGACAGTTCATACTGAAAGGGTGTTATAATTTTTATAGTAATAGGCGACCTTACGATGTATCTTCCTCTTTGTTTTCTTGTCAAGATTTGACTCATTTAGAGTTAAGCGATTGTTCGCTAAGACTCCCGTGGACTTTCAATGGCTTCAGGATGTTGAGGACCCTTAATATTAAGAGAGTTACTGTGGCCCGAGATGAATTGGAAAAGATGATTGTTTGTAGTCCTTTGCTTGAGAGATTAACTCTACGTGACTTACAGGGTATCACCCGTCTCAAGATTCACGCACCAAATCTCCAATTCCTTAAAGTTCGAGGAGATTTTGAAGATGTTGAACTTACGAATACCTTGAATCTTGTTCATGTTTCACTTGACCGAAATAGGGCTCATTGGAGAGGGGACAGACTGCTCAACTCTTGTGTTAAGTTGCCTTATATCAAAAGGCTCACAATTGAGGGTTGCATTTTAGAGTCTGCTACTGGTTCCTTGCCGAAGGAGCTGCGTAGACCATGTCTATATCTGAACTTTCTTTCTATAAACATACGCTTGAATAACTTGGAGGAGATTTCAACTGTTATGTGCCTTCTCAGAAGCTCACCTGCTCTGCAGGAACTAGAAATTTCAGTTCGCCAACAATATGAGGAGGCTACTACCGCTAGAGAAGCAACTCCTTGGTTAAATGACAACCAGAATTGGGAATTCAGCAAACTGCAACGTGTGAAAGTAACCGGCTTCTCTGGTGCCCAAGCTGAAGTAGACTTCATCAAATTTCTGTTTTTAAGCTCCCCTGCTCTCCAAGAACTAGAAGTTTCAGTTTACCAAAAAGACGAGACTGTTGTAGGAGAAGTGGACTATTCTTGGTTAAATAACGACCAAAATTTTGCACACACCCAACTGCGACTTTTGAAAGTAACCGACTTTTGTGGTGAAGTAGAATTCATCAAGTTTCTGCTTTTAAGCTCCCCTGCTCTTCAAGAACTACATATTTTGTTTAGAGAGAATGATCATAAGAACAAGATAAGAAACAAGTGGTTAGACGATGCTGCTATGGGAGATGAGGACAACCGGAATTGCACATTCACCCAACTTCGAGTTATGAACATTAGTGGCTTTTTTGGTGCCAAAGCTGAAGTAGGTTTCATCAGATTTTTGCTTACAAGTTCACCTATGCTTGAGAGGATAACTCTTCAACCTGCTTCTGCCAAAGTTCCTTGGGAAGTAAGTAAGCTGTTGAACGAGTTCAAGTCCAACTCGGTACATACAAATTTGAAGTTGTTGGACCCATTAAATCCTTCTGCTGACTACCACTCCCGAGGCTCTAGATGTAGAACCCCACTTTGTTCTGACTACTCCTGTTATGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD LRR_2

Protein Analysis

567

Amino Acids

65.58

Weight (kDa)

8.75

Isoelectric Point (pI)

48.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 23 - 62 5.7e-07 F-box domain
LRR_At1g61320_AtMIF1 PF23622 94 - 217 6.8e-12 At1g61320/AtMIF1, LRR domain
LRR_At5g56370 PF24758 103 - 320 1.2e-15 FBD-associated F-box protein At5g56370, LRR repeats
LRR_At1g61320_AtMIF1 PF23622 208 - 389 1.5e-09 At1g61320/AtMIF1, LRR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 369
AarI CACCTGC 1 cut(s) 946
Acc36I ACCTGC 2 cut(s) 946, 1540
AccB1I GGYRCC 2 cut(s) 1079, 1457
AccI GTMKAC 2 cut(s) 844, 1095
AciI CCGC 1 cut(s) 993
AclWI GGATC 1 cut(s) 234
AcsI RAATTY 7 cut(s) 99, 957, 1037, 1106, 1216, 1274, 1603
AcuI CTGAAG 3 cut(s) 472, 1110, 1488
AfaI GTAC 1 cut(s) 1596
AfiI CCNNNNNNNGG 2 cut(s) 721, 1033
AflIII ACRYGT 1 cut(s) 1054
AjiI CACGTC 1 cut(s) 214
AjnI CCWGG 1 cut(s) 247
AjuI GAANNNNNNNTTGG 2 cut(s) 41, 73
AluBI AGCT 8 cut(s) 271, 838, 933, 1088, 1122, 1299, 1466, 1567
AluI AGCT 8 cut(s) 271, 838, 933, 1088, 1122, 1299, 1466, 1567
Alw26I GTCTC 2 cut(s) 617, 1163
AlwI GGATC 1 cut(s) 234
Ama87I CYCGRG 2 cut(s) 531, 1650
AoxI GGCC 1 cut(s) 528
ApeKI GCWGC 2 cut(s) 838, 1382
ApoI RAATTY 7 cut(s) 99, 957, 1037, 1106, 1216, 1274, 1603
ArsI GACNNNNNNTTYG 2 cut(s) 1202, 1234
Asp700I GAANNNNTTC 1 cut(s) 1577
AspS9I GGNCC 4 cut(s) 251, 501, 529, 1618
AsuHPI GGTGA 6 cut(s) 236, 598, 927, 1277, 1411, 1494
AvaI CYCGRG 2 cut(s) 531, 1650
AvaII GGWCC 3 cut(s) 251, 501, 1618
BaeGI GKGCMC 1 cut(s) 1084
BanI GGYRCC 2 cut(s) 1079, 1457
BanII GRGCYC 1 cut(s) 727
BbsI GAAGAC 2 cut(s) 16, 140
BbvI GCAGC 2 cut(s) 825, 1369
BccI CCATC 2 cut(s) 46, 306
BcgI CGANNNNNNTGC 2 cut(s) 820, 854
BciT130I CCWGG 1 cut(s) 249
BclI TGATCA 2 cut(s) 196, 1342
BcoDI GTCTC 2 cut(s) 617, 1163
BfaI CTAG 4 cut(s) 953, 996, 1142, 1659
BfmI CTRYAG 1 cut(s) 944
BfuAI ACCTGC 2 cut(s) 946, 1540
BisI GCNGC 2 cut(s) 839, 1383
BlsI GCNGC 2 cut(s) 840, 1384
Bme1390I CCNGG 1 cut(s) 249
Bme18I GGWCC 3 cut(s) 251, 501, 1618
BmeT110I CYCGRG 2 cut(s) 531, 1650
BmgBI CACGTC 1 cut(s) 214
BmgT120I GGNCC 4 cut(s) 251, 501, 529, 1618
BmiI GGNNCC 6 cut(s) 253, 503, 823, 1081, 1459, 1620
BmrFI CCNGG 1 cut(s) 249
BmsI GCATC 2 cut(s) 115, 1369
BpiI GAAGAC 2 cut(s) 16, 140
BplI GAGNNNNNCTC 4 cut(s) 568, 600, 1507, 1539
BpmI CTGGAG 1 cut(s) 178
BpuEI CTTGAG 3 cut(s) 593, 599, 1532
BsaAI YACGTR 1 cut(s) 590
BsaJI CCNNGG 5 cut(s) 247, 470, 1010, 1550, 1651
BsaWI WCCGGW 2 cut(s) 31, 1404
BsaXI ACNNNNNCTCC 6 cut(s) 8, 38, 618, 648, 1631, 1661
Bsc4I CCNNNNNNNGG 2 cut(s) 721, 1033
Bse118I RCCGGY 1 cut(s) 1067
Bse1I ACTGG 2 cut(s) 161, 823
BseBI CCWGG 1 cut(s) 249
BseDI CCNNGG 5 cut(s) 247, 470, 1010, 1550, 1651
BseGI GGATG 2 cut(s) 130, 498
BseLI CCNNNNNNNGG 2 cut(s) 721, 1033
BseMII CTCAG 1 cut(s) 940
BseNI ACTGG 2 cut(s) 161, 823
BseRI GAGGAG 4 cut(s) 16, 669, 914, 995
BseSI GKGCMC 1 cut(s) 1084
BseXI GCAGC 2 cut(s) 825, 1369
BsgI GTGCAG 1 cut(s) 261
BshFI GGCC 1 cut(s) 530
BshNI GGYRCC 2 cut(s) 1079, 1457
BsiHKCI CYCGRG 2 cut(s) 531, 1650
BsiSI CCGG 3 cut(s) 32, 1068, 1405
BslFI GGGAC 2 cut(s) 237, 752
BslI CCNNNNNNNGG 2 cut(s) 721, 1033
BsmAI GTCTC 2 cut(s) 617, 1163
BsmBI CGTCTC 1 cut(s) 617
BsmFI GGGAC 2 cut(s) 237, 752
BsnI GGCC 1 cut(s) 530
BsoBI CYCGRG 2 cut(s) 531, 1650
Bsp1286I GDGCHC 2 cut(s) 727, 1084
Bsp143I GATC 4 cut(s) 117, 196, 226, 1342
BspACI CCGC 1 cut(s) 993
BspANI GGCC 1 cut(s) 530
BspCNI CTCAG 1 cut(s) 939
BspHI TCATGA 1 cut(s) 199
BspLI GGNNCC 6 cut(s) 253, 503, 823, 1081, 1459, 1620
BspMAI CTGCAG 1 cut(s) 948
BspMI ACCTGC 2 cut(s) 946, 1540
BspPI GGATC 1 cut(s) 234
BspQI GCTCTTC 1 cut(s) 1314
BspT107I GGYRCC 2 cut(s) 1079, 1457
BsrFI RCCGGY 1 cut(s) 1067
BsrI ACTGG 2 cut(s) 161, 823
BssAI RCCGGY 1 cut(s) 1067
BssECI CCNNGG 5 cut(s) 247, 470, 1010, 1550, 1651
BssMI GATC 4 cut(s) 117, 196, 226, 1342
BssT1I CCWWGG 2 cut(s) 1010, 1550
Bst2UI CCWGG 1 cut(s) 249
Bst4CI ACNGT 5 cut(s) 221, 348, 526, 913, 1174
Bst6I CTCTTC 2 cut(s) 1314, 1530
BstBAI YACGTR 1 cut(s) 590
BstDEI CTNAG 2 cut(s) 461, 926
BstDSI CCRYGG 1 cut(s) 470
BstF5I GGATG 2 cut(s) 130, 498
BstKTI GATC 4 cut(s) 120, 199, 229, 1345
BstMAI GTCTC 2 cut(s) 617, 1163
BstMBI GATC 4 cut(s) 117, 196, 226, 1342
BstMWI GCNNNNNNNGC 3 cut(s) 835, 992, 1001
BstNI CCWGG 1 cut(s) 249
BstSCI CCNGG 1 cut(s) 247
BstSFI CTRYAG 1 cut(s) 944
BstSLI GKGCMC 1 cut(s) 1084
BstV1I GCAGC 2 cut(s) 825, 1369
BstV2I GAAGAC 2 cut(s) 16, 140
BsuRI GGCC 1 cut(s) 530
BtgI CCRYGG 1 cut(s) 470
BtrI CACGTC 1 cut(s) 214
BtsCI GGATG 2 cut(s) 130, 498
BtsIMutI CAGTG 1 cut(s) 226
BveI ACCTGC 2 cut(s) 946, 1540
CciI TCATGA 1 cut(s) 199
Cfr10I RCCGGY 1 cut(s) 1067
Cfr13I GGNCC 4 cut(s) 251, 501, 529, 1618
Csp6I GTAC 1 cut(s) 1595
CviAII CATG 3 cut(s) 200, 701, 849
CviQI GTAC 1 cut(s) 1595
DdeI CTNAG 2 cut(s) 461, 926
DpnI GATC 4 cut(s) 119, 198, 228, 1344
DpnII GATC 4 cut(s) 117, 196, 226, 1342
Eam1104I CTCTTC 2 cut(s) 1314, 1530
EarI CTCTTC 2 cut(s) 1314, 1530
Eco130I CCWWGG 2 cut(s) 1010, 1550
Eco24I GRGCYC 1 cut(s) 727
Eco47I GGWCC 3 cut(s) 251, 501, 1618
Eco57I CTGAAG 3 cut(s) 472, 1110, 1488
Eco88I CYCGRG 2 cut(s) 531, 1650
EcoO109I RGGNCCY 1 cut(s) 501
EcoRI GAATTC 2 cut(s) 1037, 1274
EcoRII CCWGG 1 cut(s) 247
EcoT14I CCWWGG 2 cut(s) 1010, 1550
EcoT38I GRGCYC 1 cut(s) 727
ErhI CCWWGG 2 cut(s) 1010, 1550
Esp3I CGTCTC 1 cut(s) 617
FaeI CATG 3 cut(s) 203, 704, 852
FaqI GGGAC 2 cut(s) 237, 752
FatI CATG 3 cut(s) 199, 700, 848
FbaI TGATCA 2 cut(s) 196, 1342
FblI GTMKAC 2 cut(s) 844, 1095
Fnu4HI GCNGC 2 cut(s) 839, 1383
FokI GGATG 2 cut(s) 137, 505
FriOI GRGCYC 1 cut(s) 727
Fsp4HI GCNGC 2 cut(s) 839, 1383
FspBI CTAG 4 cut(s) 953, 996, 1142, 1659
GluI GCNGC 2 cut(s) 839, 1383
GsuI CTGGAG 1 cut(s) 178
HaeIII GGCC 1 cut(s) 530
HapII CCGG 3 cut(s) 32, 1068, 1405
Hin1II CATG 3 cut(s) 203, 704, 852
HindIII AAGCTT 1 cut(s) 269
HinfI GANTC 6 cut(s) 316, 433, 465, 619, 691, 809
HpaII CCGG 3 cut(s) 32, 1068, 1405
HphI GGTGA 6 cut(s) 236, 598, 927, 1277, 1411, 1494
Hpy166II GTNNAC 7 cut(s) 217, 474, 845, 1096, 1156, 1189, 1502
Hpy188I TCNGA 5 cut(s) 344, 861, 929, 1484, 1684
Hpy188III TCNNGA 9 cut(s) 6, 200, 330, 425, 490, 616, 1313, 1650, 1659
Hpy8I GTNNAC 7 cut(s) 217, 474, 845, 1096, 1156, 1189, 1502
HpyAV CCTTC 5 cut(s) 39, 192, 826, 932, 1641
HpyCH4III ACNGT 5 cut(s) 221, 348, 526, 913, 1174
HpyCH4IV ACGT 4 cut(s) 213, 231, 589, 1054
HpyCH4V TGCA 7 cut(s) 128, 278, 801, 946, 1051, 1223, 1413
HpyF10VI GCNNNNNNNGC 3 cut(s) 835, 992, 1001
HpyF3I CTNAG 2 cut(s) 461, 926
HpySE526I ACGT 4 cut(s) 213, 231, 589, 1054
Hsp92II CATG 3 cut(s) 203, 704, 852
Ksp22I TGATCA 2 cut(s) 196, 1342
Kzo9I GATC 4 cut(s) 117, 196, 226, 1342
LguI GCTCTTC 1 cut(s) 1314
LmnI GCTCC 3 cut(s) 835, 1127, 1304
Lsp1109I GCAGC 2 cut(s) 825, 1369
LweI GCATC 2 cut(s) 115, 1369
MaeI CTAG 4 cut(s) 953, 996, 1142, 1659
MaeII ACGT 4 cut(s) 213, 231, 589, 1054
MaeIII GTNAC 5 cut(s) 88, 520, 590, 1063, 1249
MalI GATC 4 cut(s) 119, 198, 228, 1344
MboI GATC 4 cut(s) 117, 196, 226, 1342
MboII GAAGA 7 cut(s) 21, 61, 145, 396, 677, 1301, 1517
MfeI CAATTG 1 cut(s) 789
MhlI GDGCHC 2 cut(s) 727, 1084
MlyI GAGTC 3 cut(s) 427, 459, 818
MmeI TCCRAC 3 cut(s) 39, 1596, 1611
MroXI GAANNNNTTC 1 cut(s) 1577
MspI CCGG 3 cut(s) 32, 1068, 1405
MspR9I CCNGG 1 cut(s) 249
MunI CAATTG 1 cut(s) 789
MvaI CCWGG 1 cut(s) 249
MwoI GCNNNNNNNGC 3 cut(s) 835, 992, 1001
NdeII GATC 4 cut(s) 117, 196, 226, 1342
NlaIII CATG 3 cut(s) 203, 704, 852
NlaIV GGNNCC 6 cut(s) 253, 503, 823, 1081, 1459, 1620
NmuCI GTSAC 1 cut(s) 590
PagI TCATGA 1 cut(s) 199
PaqCI CACCTGC 1 cut(s) 946
PciSI GCTCTTC 1 cut(s) 1314
PdmI GAANNNNTTC 1 cut(s) 1577
PfeI GAWTC 3 cut(s) 316, 619, 691
PflFI GACNNNGTC 1 cut(s) 849
PkrI GCNGC 2 cut(s) 840, 1384
PleI GAGTC 3 cut(s) 427, 459, 817
PpsI GAGTC 3 cut(s) 427, 459, 817
Ppu21I YACGTR 1 cut(s) 590
PpuMI RGGWCCY 1 cut(s) 501
PsiI TTATAA 1 cut(s) 369
Psp5II RGGWCCY 1 cut(s) 501
Psp6I CCWGG 1 cut(s) 247
PspGI CCWGG 1 cut(s) 247
PspN4I GGNNCC 6 cut(s) 253, 503, 823, 1081, 1459, 1620
PspPI GGNCC 4 cut(s) 251, 501, 529, 1618
PspPPI RGGWCCY 1 cut(s) 501
PstI CTGCAG 1 cut(s) 948
PsyI GACNNNGTC 1 cut(s) 849
RsaI GTAC 1 cut(s) 1596
RsaNI GTAC 1 cut(s) 1595
SapI GCTCTTC 1 cut(s) 1314
SatI GCNGC 2 cut(s) 839, 1383
Sau3AI GATC 4 cut(s) 117, 196, 226, 1342
Sau96I GGNCC 4 cut(s) 251, 501, 529, 1618
SchI GAGTC 3 cut(s) 427, 459, 818
ScrFI CCNGG 1 cut(s) 249
SduI GDGCHC 2 cut(s) 727, 1084
SfaNI GCATC 2 cut(s) 115, 1369
SfcI CTRYAG 1 cut(s) 944
SinI GGWCC 3 cut(s) 251, 501, 1618
SmlI CTYRAG 3 cut(s) 572, 614, 1511
SmoI CTYRAG 3 cut(s) 572, 614, 1511
SsiI CCGC 1 cut(s) 993
SspI AATATT 1 cut(s) 511
SspMI CTAG 4 cut(s) 953, 996, 1142, 1659
StyD4I CCNGG 1 cut(s) 247
StyI CCWWGG 2 cut(s) 1010, 1550
TaaI ACNGT 5 cut(s) 221, 348, 526, 913, 1174
TaiI ACGT 4 cut(s) 216, 234, 592, 1057
TaqI TCGA 2 cut(s) 652, 1429
TaqII GACCGA 1 cut(s) 729
TfiI GAWTC 3 cut(s) 316, 619, 691
TscAI CASTG 1 cut(s) 226
TseFI GTSAC 1 cut(s) 590
TseI GCWGC 2 cut(s) 838, 1382
Tsp45I GTSAC 1 cut(s) 590
TspDTI ATGAA 8 cut(s) 216, 340, 552, 689, 1090, 1267, 1451, 1468
TspRI CASTG 1 cut(s) 226
Tth111I GACNNNGTC 1 cut(s) 849
VpaK11BI GGWCC 3 cut(s) 251, 501, 1618
XapI RAATTY 7 cut(s) 99, 957, 1037, 1106, 1216, 1274, 1603
XbaI TCTAGA 1 cut(s) 1658
XmiI GTMKAC 2 cut(s) 844, 1095
XmnI GAANNNNTTC 1 cut(s) 1577
XspI CTAG 4 cut(s) 953, 996, 1142, 1659
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.