RLG00000034607

F-box FBD LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0003942
Physical Location & Seq
Reverse (-)
45927061 .. 45928320
1260 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000034607

Sequence Viewer

Length: 1260 bp
ATGATTGTTTGTAGTCCTTTGCTTGAGAGATTAACCCTACGTGACTTACAGGGTATCACCCGTCTCAAGATTCACGCACCGAATCTCCAATTCCTTAAAGTTCGAGGAGATTTTGAAGATGTTGAACTTACGAATACCTTGCATCTTGTTCATGTTTCACTTGACCGAAATAGGGCTCATGGGAGAGGGGACAGACTGCTCAACTCGTGTGTTAAGTTGCCTTATATCAAAAGGCTCACAATTGAGGGTTGCATTTTAGAGTGTGCTACTGGTTCCTTGCCGAAGGAGCTGCGTAGACCATGTCTATATCTGAACTTTCTTTCTATAAACATACGCTTGAATAACTTGAAGGAGATTTCAACTGCTATGTGCCTTCTCAGAAGCTCACCTGCTCTGCAGGAACTAGAAATTTCAGTTCGCCAACAATATGAGGAGGCTACTACCGCTAGAGAAGCAACTCCTTGGTTAAATGACAACCAGAATTGGGAATTCAGCAAACTGCAACGTGTGAAAGTAACCGGCTTTTCTGGTGCCCAAGCTGAAGTAGACTTCATCAAATTTCTGTTTTTAAGCTGCCCTGCTCTCCAAGAACTAGAAGTTTCAGTTTACCAAAAAGACAAGACTGTTGTAGGAGAAGTGGACTATTCTTGGTTAAATAACGACCAAAATTTTGCACACACCCAACTGCGACTTTTGAAAGTAACCGACTTTTGTGGTGAAGTAGAATTCATCAAGTTTCTGCTTTTAAGTTCCCCTGCTCTTCAAGAACTACATATTTTGTTTAGAGAGAATGATCATAAGAACAAGATGAGAAACAAGTGGTTAGACGATGCTGCTATGGGAGATGAGGACAACCGGAATTGCACATTCACCCAACTTCGAGTTACGAACATTAGTGGCTTTTCTGGTGCCAAAGCTGAAGTAGGTTTCATCAAATTTTTGCTTACAAGTTCACCCATGCTTGAGAGGATAACTCTTCAACCTGCTTCTGCCAAAGTTTCTTGGGAACTAAGTAAGTTGTTGACCGAGTTCAAGTCCAACTCGGTACAGGCTAATTTAAAGTTGTTGGACCCAGTAAATCCTTCAGATGACTGCCACTCCTCCCGAGCCGCTAGATGTAGATCCCCAGACTACCACTCCTCCCGAGCCGCTAGATGTAGATCCCCAGTTTATTCTGACTCCTCTGAATCAGCCTCCAGATCCCCAGTTTATTCTGATTCCTCTGAAACAGCCTCCAGATCCCCATTCGACTATGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD LRR_2

Protein Analysis

420

Amino Acids

47.59

Weight (kDa)

7.09

Isoelectric Point (pI)

54.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At1g61320_AtMIF1 PF23622 8 - 206 1.4e-10 At1g61320/AtMIF1, LRR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 397
Acc36I ACCTGC 2 cut(s) 397, 991
AccB1I GGYRCC 2 cut(s) 530, 908
AccI GTMKAC 2 cut(s) 295, 546
AciI CCGC 3 cut(s) 444, 1110, 1149
AclWI GGATC 4 cut(s) 1116, 1155, 1194, 1233
AcsI RAATTY 6 cut(s) 408, 488, 557, 667, 725, 935
AcuI CTGAAG 3 cut(s) 561, 939, 1068
AfaI GTAC 1 cut(s) 1047
AfiI CCNNNNNNNGG 2 cut(s) 172, 484
AflIII ACRYGT 1 cut(s) 505
AgsI TTSAA 9 cut(s) 116, 125, 340, 349, 360, 697, 764, 980, 1033
AluBI AGCT 5 cut(s) 289, 384, 539, 573, 917
AluI AGCT 5 cut(s) 289, 384, 539, 573, 917
Alw26I GTCTC 1 cut(s) 68
AlwI GGATC 4 cut(s) 1116, 1155, 1194, 1233
Ama87I CYCGRG 2 cut(s) 1104, 1143
ApeKI GCWGC 3 cut(s) 289, 573, 833
ApoI RAATTY 6 cut(s) 408, 488, 557, 667, 725, 935
ArsI GACNNNNNNTTYG 2 cut(s) 653, 685
AspS9I GGNCC 1 cut(s) 1069
AsuHPI GGTGA 5 cut(s) 49, 378, 728, 862, 945
AvaI CYCGRG 2 cut(s) 1104, 1143
AvaII GGWCC 1 cut(s) 1069
BaeGI GKGCMC 1 cut(s) 535
BanI GGYRCC 2 cut(s) 530, 908
BanII GRGCYC 1 cut(s) 178
BauI CACGAG 1 cut(s) 205
BbvI GCAGC 3 cut(s) 276, 560, 820
BcgI CGANNNNNNTGC 4 cut(s) 121, 155, 271, 305
BclI TGATCA 1 cut(s) 793
BcoDI GTCTC 1 cut(s) 68
BfaI CTAG 5 cut(s) 404, 447, 593, 1113, 1152
BfmI CTRYAG 1 cut(s) 395
BfuAI ACCTGC 2 cut(s) 397, 991
BisI GCNGC 5 cut(s) 290, 574, 834, 1110, 1149
BlsI GCNGC 5 cut(s) 291, 575, 835, 1111, 1150
Bme18I GGWCC 1 cut(s) 1069
BmeT110I CYCGRG 2 cut(s) 1104, 1143
BmgT120I GGNCC 1 cut(s) 1069
BmiI GGNNCC 4 cut(s) 274, 532, 910, 1071
BmrI ACTGGG 3 cut(s) 1067, 1160, 1199
BmsI GCATC 2 cut(s) 151, 820
BmuI ACTGGG 3 cut(s) 1067, 1160, 1199
BplI GAGNNNNNCTC 2 cut(s) 958, 990
BpmI CTGGAG 2 cut(s) 1180, 1219
BpuEI CTTGAG 3 cut(s) 44, 50, 983
BsaAI YACGTR 1 cut(s) 41
BsaBI GATNNNNATC 2 cut(s) 1120, 1159
BsaJI CCNNGG 1 cut(s) 461
BsaWI WCCGGW 1 cut(s) 855
BsaXI ACNNNNNCTCC 8 cut(s) 69, 99, 1082, 1112, 1121, 1124, 1151, 1154
Bsc4I CCNNNNNNNGG 2 cut(s) 172, 484
Bse118I RCCGGY 1 cut(s) 518
Bse1I ACTGG 4 cut(s) 274, 1073, 1166, 1205
Bse8I GATNNNNATC 2 cut(s) 1120, 1159
BseDI CCNNGG 1 cut(s) 461
BseJI GATNNNNATC 2 cut(s) 1120, 1159
BseLI CCNNNNNNNGG 2 cut(s) 172, 484
BseMII CTCAG 1 cut(s) 391
BseNI ACTGG 4 cut(s) 274, 1073, 1166, 1205
BseRI GAGGAG 5 cut(s) 120, 446, 1090, 1129, 1171
BseSI GKGCMC 1 cut(s) 535
BseXI GCAGC 3 cut(s) 276, 560, 820
BshNI GGYRCC 2 cut(s) 530, 908
BsiHKCI CYCGRG 2 cut(s) 1104, 1143
BsiSI CCGG 2 cut(s) 519, 856
BslFI GGGAC 1 cut(s) 203
BslI CCNNNNNNNGG 2 cut(s) 172, 484
BsmAI GTCTC 1 cut(s) 68
BsmBI CGTCTC 1 cut(s) 68
BsmFI GGGAC 1 cut(s) 203
BsoBI CYCGRG 2 cut(s) 1104, 1143
Bsp1286I GDGCHC 2 cut(s) 178, 535
Bsp143I GATC 5 cut(s) 793, 1121, 1160, 1199, 1238
BspACI CCGC 3 cut(s) 444, 1110, 1149
BspCNI CTCAG 1 cut(s) 390
BspLI GGNNCC 4 cut(s) 274, 532, 910, 1071
BspMAI CTGCAG 1 cut(s) 399
BspMI ACCTGC 2 cut(s) 397, 991
BspPI GGATC 4 cut(s) 1116, 1155, 1194, 1233
BspQI GCTCTTC 1 cut(s) 765
BspT107I GGYRCC 2 cut(s) 530, 908
BsrFI RCCGGY 1 cut(s) 518
BsrI ACTGG 4 cut(s) 274, 1073, 1166, 1205
BssAI RCCGGY 1 cut(s) 518
BssECI CCNNGG 1 cut(s) 461
BssMI GATC 5 cut(s) 793, 1121, 1160, 1199, 1238
BssSI CACGAG 1 cut(s) 205
BssT1I CCWWGG 1 cut(s) 461
Bst2BI CACGAG 1 cut(s) 205
Bst4CI ACNGT 1 cut(s) 625
Bst6I CTCTTC 2 cut(s) 765, 981
BstBAI YACGTR 1 cut(s) 41
BstDEI CTNAG 2 cut(s) 377, 1010
BstKTI GATC 5 cut(s) 796, 1124, 1163, 1202, 1241
BstMAI GTCTC 1 cut(s) 68
BstMBI GATC 5 cut(s) 793, 1121, 1160, 1199, 1238
BstMWI GCNNNNNNNGC 3 cut(s) 286, 443, 452
BstSFI CTRYAG 1 cut(s) 395
BstSLI GKGCMC 1 cut(s) 535
BstV1I GCAGC 3 cut(s) 276, 560, 820
BstX2I RGATCY 4 cut(s) 1121, 1160, 1199, 1238
BstYI RGATCY 4 cut(s) 1121, 1160, 1199, 1238
BveI ACCTGC 2 cut(s) 397, 991
Cfr10I RCCGGY 1 cut(s) 518
Cfr13I GGNCC 1 cut(s) 1069
Csp6I GTAC 1 cut(s) 1046
CviAII CATG 4 cut(s) 152, 179, 300, 958
CviQI GTAC 1 cut(s) 1046
DdeI CTNAG 2 cut(s) 377, 1010
DpnI GATC 5 cut(s) 795, 1123, 1162, 1201, 1240
DpnII GATC 5 cut(s) 793, 1121, 1160, 1199, 1238
DraI TTTAAA 1 cut(s) 1059
Eam1104I CTCTTC 2 cut(s) 765, 981
EarI CTCTTC 2 cut(s) 765, 981
Eco130I CCWWGG 1 cut(s) 461
Eco24I GRGCYC 1 cut(s) 178
Eco47I GGWCC 1 cut(s) 1069
Eco57I CTGAAG 3 cut(s) 561, 939, 1068
Eco88I CYCGRG 2 cut(s) 1104, 1143
EcoRI GAATTC 2 cut(s) 488, 725
EcoT14I CCWWGG 1 cut(s) 461
EcoT38I GRGCYC 1 cut(s) 178
ErhI CCWWGG 1 cut(s) 461
Esp3I CGTCTC 1 cut(s) 68
FaeI CATG 4 cut(s) 155, 182, 303, 961
FaqI GGGAC 1 cut(s) 203
FatI CATG 4 cut(s) 151, 178, 299, 957
FbaI TGATCA 1 cut(s) 793
FblI GTMKAC 2 cut(s) 295, 546
Fnu4HI GCNGC 5 cut(s) 290, 574, 834, 1110, 1149
FriOI GRGCYC 1 cut(s) 178
Fsp4HI GCNGC 5 cut(s) 290, 574, 834, 1110, 1149
FspBI CTAG 5 cut(s) 404, 447, 593, 1113, 1152
GluI GCNGC 5 cut(s) 290, 574, 834, 1110, 1149
GsuI CTGGAG 2 cut(s) 1180, 1219
HapII CCGG 2 cut(s) 519, 856
Hin1II CATG 4 cut(s) 155, 182, 303, 961
HincII GTYRAC 1 cut(s) 1023
HindII GTYRAC 1 cut(s) 1023
HinfI GANTC 5 cut(s) 70, 82, 1178, 1187, 1217
HpaII CCGG 2 cut(s) 519, 856
HphI GGTGA 5 cut(s) 49, 378, 728, 862, 945
Hpy166II GTNNAC 6 cut(s) 296, 547, 607, 640, 953, 1023
Hpy188I TCNGA 7 cut(s) 312, 380, 1087, 1177, 1186, 1216, 1225
Hpy188III TCNNGA 6 cut(s) 67, 764, 1104, 1143, 1197, 1236
Hpy8I GTNNAC 6 cut(s) 296, 547, 607, 640, 953, 1023
HpyAV CCTTC 4 cut(s) 277, 343, 383, 1092
HpyCH4III ACNGT 1 cut(s) 625
HpyCH4IV ACGT 2 cut(s) 40, 505
HpyCH4V TGCA 6 cut(s) 142, 252, 397, 502, 674, 864
HpyF10VI GCNNNNNNNGC 3 cut(s) 286, 443, 452
HpyF3I CTNAG 2 cut(s) 377, 1010
HpySE526I ACGT 2 cut(s) 40, 505
Hsp92II CATG 4 cut(s) 155, 182, 303, 961
Ksp22I TGATCA 1 cut(s) 793
Kzo9I GATC 5 cut(s) 793, 1121, 1160, 1199, 1238
LguI GCTCTTC 1 cut(s) 765
LmnI GCTCC 1 cut(s) 286
Lsp1109I GCAGC 3 cut(s) 276, 560, 820
LweI GCATC 2 cut(s) 151, 820
MaeI CTAG 5 cut(s) 404, 447, 593, 1113, 1152
MaeII ACGT 2 cut(s) 40, 505
MaeIII GTNAC 4 cut(s) 41, 514, 700, 883
MalI GATC 5 cut(s) 795, 1123, 1162, 1201, 1240
MboI GATC 5 cut(s) 793, 1121, 1160, 1199, 1238
MboII GAAGA 3 cut(s) 128, 752, 968
MfeI CAATTG 1 cut(s) 240
MflI RGATCY 4 cut(s) 1121, 1160, 1199, 1238
MhlI GDGCHC 2 cut(s) 178, 535
MlyI GAGTC 1 cut(s) 1172
MmeI TCCRAC 2 cut(s) 1047, 1062
MseI TTAA 8 cut(s) 32, 96, 213, 467, 569, 653, 746, 1058
MspI CCGG 2 cut(s) 519, 856
MunI CAATTG 1 cut(s) 240
MwoI GCNNNNNNNGC 3 cut(s) 286, 443, 452
NdeII GATC 5 cut(s) 793, 1121, 1160, 1199, 1238
NlaIII CATG 4 cut(s) 155, 182, 303, 961
NlaIV GGNNCC 4 cut(s) 274, 532, 910, 1071
NmuCI GTSAC 1 cut(s) 41
PaqCI CACCTGC 1 cut(s) 397
PciSI GCTCTTC 1 cut(s) 765
PfeI GAWTC 4 cut(s) 70, 82, 1187, 1217
PflFI GACNNNGTC 1 cut(s) 300
PkrI GCNGC 5 cut(s) 291, 575, 835, 1111, 1150
PleI GAGTC 1 cut(s) 1172
PpsI GAGTC 1 cut(s) 1172
Ppu21I YACGTR 1 cut(s) 41
PspN4I GGNNCC 4 cut(s) 274, 532, 910, 1071
PspPI GGNCC 1 cut(s) 1069
PstI CTGCAG 1 cut(s) 399
PsuI RGATCY 4 cut(s) 1121, 1160, 1199, 1238
PsyI GACNNNGTC 1 cut(s) 300
RsaI GTAC 1 cut(s) 1047
RsaNI GTAC 1 cut(s) 1046
SapI GCTCTTC 1 cut(s) 765
SaqAI TTAA 8 cut(s) 32, 96, 213, 467, 569, 653, 746, 1058
SatI GCNGC 5 cut(s) 290, 574, 834, 1110, 1149
Sau3AI GATC 5 cut(s) 793, 1121, 1160, 1199, 1238
Sau96I GGNCC 1 cut(s) 1069
SchI GAGTC 1 cut(s) 1172
SduI GDGCHC 2 cut(s) 178, 535
SfaNI GCATC 2 cut(s) 151, 820
SfcI CTRYAG 1 cut(s) 395
SinI GGWCC 1 cut(s) 1069
SmlI CTYRAG 3 cut(s) 23, 65, 962
SmoI CTYRAG 3 cut(s) 23, 65, 962
SsiI CCGC 3 cut(s) 444, 1110, 1149
SspMI CTAG 5 cut(s) 404, 447, 593, 1113, 1152
StyI CCWWGG 1 cut(s) 461
TaaI ACNGT 1 cut(s) 625
TaiI ACGT 2 cut(s) 43, 508
TaqI TCGA 3 cut(s) 103, 880, 1248
TaqII GACCGA 2 cut(s) 180, 1040
TauI GCSGC 2 cut(s) 1112, 1151
TfiI GAWTC 4 cut(s) 70, 82, 1187, 1217
Tru1I TTAA 8 cut(s) 32, 96, 213, 467, 569, 653, 746, 1058
Tru9I TTAA 8 cut(s) 32, 96, 213, 467, 569, 653, 746, 1058
TseFI GTSAC 1 cut(s) 41
TseI GCWGC 3 cut(s) 289, 573, 833
Tsp45I GTSAC 1 cut(s) 41
TspDTI ATGAA 4 cut(s) 140, 541, 718, 919
Tth111I GACNNNGTC 1 cut(s) 300
VpaK11BI GGWCC 1 cut(s) 1069
XapI RAATTY 6 cut(s) 408, 488, 557, 667, 725, 935
XmiI GTMKAC 2 cut(s) 295, 546
XspI CTAG 5 cut(s) 404, 447, 593, 1113, 1152
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.