TPLATE-like
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 977 |
| AatII | GACGTC | 3 cut(s) | 174, 417, 1104 |
| Acc36I | ACCTGC | 2 cut(s) | 556, 1915 |
| AccB1I | GGYRCC | 1 cut(s) | 959 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 727 |
| AccBSI | CCGCTC | 3 cut(s) | 619, 670, 1062 |
| AccII | CGCG | 6 cut(s) | 551, 752, 858, 969, 1069, 1226 |
| AclWI | GGATC | 7 cut(s) | 720, 803, 816, 1313, 1449, 1727, 1740 |
| AcoI | YGGCCR | 4 cut(s) | 3, 495, 588, 964 |
| AcsI | RAATTY | 1 cut(s) | 1438 |
| AcuI | CTGAAG | 1 cut(s) | 1845 |
| AcvI | CACGTG | 2 cut(s) | 532, 1259 |
| AcyI | GRCGYC | 6 cut(s) | 66, 171, 414, 960, 1101, 1335 |
| AdeI | CACNNNGTG | 1 cut(s) | 532 |
| AfaI | GTAC | 3 cut(s) | 398, 836, 1772 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 328, 373, 605, 727 |
| AflIII | ACRYGT | 1 cut(s) | 1764 |
| AgsI | TTSAA | 4 cut(s) | 294, 850, 1473, 1689 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 413 |
| AjnI | CCWGG | 1 cut(s) | 1786 |
| Alw21I | GWGCWC | 4 cut(s) | 167, 336, 1507, 1713 |
| Alw26I | GTCTC | 6 cut(s) | 355, 626, 631, 1123, 1229, 1471 |
| AlwI | GGATC | 7 cut(s) | 720, 803, 816, 1313, 1449, 1727, 1740 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 456 |
| Ama87I | CYCGRG | 2 cut(s) | 327, 917 |
| AoxI | GGCC | 9 cut(s) | 3, 495, 588, 743, 964, 1300, 1548, 1587, 1719 |
| ApeKI | GCWGC | 8 cut(s) | 435, 438, 441, 444, 447, 450, 453, 456 |
| ApoI | RAATTY | 1 cut(s) | 1438 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 993, 1025 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 113, 737 |
| AspLEI | GCGC | 6 cut(s) | 509, 551, 553, 962, 1071, 1109 |
| AspS9I | GGNCC | 5 cut(s) | 24, 718, 743, 1300, 1548 |
| AsuC2I | CCSGG | 1 cut(s) | 499 |
| AsuHPI | GGTGA | 7 cut(s) | 79, 169, 518, 670, 1066, 1222, 1708 |
| AsuII | TTCGAA | 1 cut(s) | 1343 |
| AvaI | CYCGRG | 2 cut(s) | 327, 917 |
| AvaII | GGWCC | 2 cut(s) | 24, 718 |
| BalI | TGGCCA | 2 cut(s) | 5, 590 |
| BamHI | GGATCC | 2 cut(s) | 808, 1732 |
| BanI | GGYRCC | 1 cut(s) | 959 |
| BanII | GRGCYC | 2 cut(s) | 167, 336 |
| BarI | GAAGNNNNNNTAC | 4 cut(s) | 821, 853, 1271, 1303 |
| BauI | CACGAG | 2 cut(s) | 554, 1268 |
| BbrPI | CACGTG | 2 cut(s) | 532, 1259 |
| BbsI | GAAGAC | 5 cut(s) | 244, 268, 408, 1279, 1620 |
| Bbv12I | GWGCWC | 4 cut(s) | 167, 336, 1507, 1713 |
| BbvI | GCAGC | 8 cut(s) | 443, 447, 450, 453, 456, 459, 462, 465 |
| BccI | CCATC | 5 cut(s) | 251, 729, 1142, 1234, 1607 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 372, 406, 1024, 1058 |
| BciT130I | CCWGG | 1 cut(s) | 1788 |
| BcnI | CCSGG | 1 cut(s) | 499 |
| BcoDI | GTCTC | 6 cut(s) | 355, 626, 631, 1123, 1229, 1471 |
| BfaI | CTAG | 1 cut(s) | 1866 |
| BfoI | RGCGCY | 3 cut(s) | 510, 963, 1110 |
| BfuAI | ACCTGC | 2 cut(s) | 556, 1915 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 1580 |
| BglII | AGATCT | 1 cut(s) | 13 |
| Bme1390I | CCNGG | 2 cut(s) | 499, 1788 |
| Bme18I | GGWCC | 2 cut(s) | 24, 718 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 413 |
| BmeT110I | CYCGRG | 2 cut(s) | 327, 917 |
| BmgT120I | GGNCC | 5 cut(s) | 24, 718, 743, 1300, 1548 |
| BmiI | GGNNCC | 7 cut(s) | 203, 719, 810, 961, 1301, 1597, 1734 |
| BmrFI | CCNGG | 2 cut(s) | 499, 1788 |
| BmsI | GCATC | 1 cut(s) | 388 |
| BpiI | GAAGAC | 5 cut(s) | 244, 268, 408, 1279, 1620 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 462, 494 |
| Bpu14I | TTCGAA | 1 cut(s) | 1343 |
| BpuEI | CTTGAG | 2 cut(s) | 143, 404 |
| BpuMI | CCSGG | 1 cut(s) | 499 |
| Bsa29I | ATCGAT | 1 cut(s) | 382 |
| BsaAI | YACGTR | 2 cut(s) | 532, 1259 |
| BsaHI | GRCGYC | 6 cut(s) | 66, 171, 414, 960, 1101, 1335 |
| BsaI | GGTCTC | 1 cut(s) | 1471 |
| BsaJI | CCNNGG | 4 cut(s) | 20, 259, 498, 1551 |
| BsaWI | WCCGGW | 2 cut(s) | 724, 1218 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 328, 373, 605, 727 |
| Bse118I | RCCGGY | 3 cut(s) | 509, 962, 1531 |
| Bse1I | ACTGG | 3 cut(s) | 288, 394, 683 |
| BseBI | CCWGG | 1 cut(s) | 1788 |
| BseCI | ATCGAT | 1 cut(s) | 382 |
| BseDI | CCNNGG | 4 cut(s) | 20, 259, 498, 1551 |
| BseGI | GGATG | 6 cut(s) | 687, 721, 931, 1056, 1134, 1404 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 328, 373, 605, 727 |
| BseNI | ACTGG | 3 cut(s) | 288, 394, 683 |
| BsePI | GCGCGC | 1 cut(s) | 549 |
| BseRI | GAGGAG | 1 cut(s) | 1139 |
| BseX3I | CGGCCG | 1 cut(s) | 964 |
| BseXI | GCAGC | 8 cut(s) | 443, 447, 450, 453, 456, 459, 462, 465 |
| Bsh1236I | CGCG | 6 cut(s) | 551, 752, 858, 969, 1069, 1226 |
| Bsh1285I | CGRYCG | 1 cut(s) | 967 |
| BshFI | GGCC | 9 cut(s) | 5, 497, 590, 745, 966, 1302, 1550, 1589, 1721 |
| BshNI | GGYRCC | 1 cut(s) | 959 |
| BshVI | ATCGAT | 1 cut(s) | 382 |
| BsiEI | CGRYCG | 1 cut(s) | 967 |
| BsiHKAI | GWGCWC | 4 cut(s) | 167, 336, 1507, 1713 |
| BsiHKCI | CYCGRG | 2 cut(s) | 327, 917 |
| BslFI | GGGAC | 2 cut(s) | 515, 731 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 328, 373, 605, 727 |
| BsmAI | GTCTC | 6 cut(s) | 355, 626, 631, 1123, 1229, 1471 |
| BsmBI | CGTCTC | 4 cut(s) | 355, 626, 1123, 1229 |
| BsmFI | GGGAC | 2 cut(s) | 515, 731 |
| BsmI | GAATGC | 2 cut(s) | 895, 1248 |
| BsnI | GGCC | 9 cut(s) | 5, 497, 590, 745, 966, 1302, 1550, 1589, 1721 |
| Bso31I | GGTCTC | 1 cut(s) | 1471 |
| BsoBI | CYCGRG | 2 cut(s) | 327, 917 |
| Bsp119I | TTCGAA | 1 cut(s) | 1343 |
| Bsp1286I | GDGCHC | 4 cut(s) | 167, 336, 1507, 1713 |
| Bsp19I | CCATGG | 1 cut(s) | 20 |
| Bsp68I | TCGCGA | 1 cut(s) | 1226 |
| BspANI | GGCC | 9 cut(s) | 5, 497, 590, 745, 966, 1302, 1550, 1589, 1721 |
| BspDI | ATCGAT | 1 cut(s) | 382 |
| BspFNI | CGCG | 6 cut(s) | 551, 752, 858, 969, 1069, 1226 |
| BspLI | GGNNCC | 7 cut(s) | 203, 719, 810, 961, 1301, 1597, 1734 |
| BspMI | ACCTGC | 2 cut(s) | 556, 1915 |
| BspPI | GGATC | 7 cut(s) | 720, 803, 816, 1313, 1449, 1727, 1740 |
| BspQI | GCTCTTC | 1 cut(s) | 609 |
| BspT104I | TTCGAA | 1 cut(s) | 1343 |
| BspT107I | GGYRCC | 1 cut(s) | 959 |
| BspTNI | GGTCTC | 1 cut(s) | 1471 |
| BsrBI | CCGCTC | 3 cut(s) | 619, 670, 1062 |
| BsrFI | RCCGGY | 3 cut(s) | 509, 962, 1531 |
| BsrI | ACTGG | 3 cut(s) | 288, 394, 683 |
| BssAI | RCCGGY | 3 cut(s) | 509, 962, 1531 |
| BssECI | CCNNGG | 4 cut(s) | 20, 259, 498, 1551 |
| BssHII | GCGCGC | 1 cut(s) | 549 |
| BssNI | GRCGYC | 6 cut(s) | 66, 171, 414, 960, 1101, 1335 |
| BssSI | CACGAG | 2 cut(s) | 554, 1268 |
| BssT1I | CCWWGG | 1 cut(s) | 20 |
| Bst2BI | CACGAG | 2 cut(s) | 554, 1268 |
| Bst2UI | CCWGG | 1 cut(s) | 1788 |
| Bst4CI | ACNGT | 6 cut(s) | 28, 91, 98, 486, 787, 1555 |
| Bst6I | CTCTTC | 6 cut(s) | 41, 609, 824, 937, 996, 1474 |
| BstACI | GRCGYC | 6 cut(s) | 66, 171, 414, 960, 1101, 1335 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 456, 1708 |
| BstBAI | YACGTR | 2 cut(s) | 532, 1259 |
| BstBI | TTCGAA | 1 cut(s) | 1343 |
| BstC8I | GCNNGC | 6 cut(s) | 61, 511, 547, 551, 964, 1446 |
| BstDEI | CTNAG | 2 cut(s) | 1173, 1800 |
| BstDSI | CCRYGG | 3 cut(s) | 20, 259, 1551 |
| BstF5I | GGATG | 6 cut(s) | 687, 721, 931, 1056, 1134, 1404 |
| BstFNI | CGCG | 6 cut(s) | 551, 752, 858, 969, 1069, 1226 |
| BstH2I | RGCGCY | 3 cut(s) | 510, 963, 1110 |
| BstHHI | GCGC | 6 cut(s) | 509, 551, 553, 962, 1071, 1109 |
| BstMAI | GTCTC | 6 cut(s) | 355, 626, 631, 1123, 1229, 1471 |
| BstMCI | CGRYCG | 1 cut(s) | 967 |
| BstNI | CCWGG | 1 cut(s) | 1788 |
| BstNSI | RCATGY | 3 cut(s) | 586, 1321, 1768 |
| BstSCI | CCNGG | 2 cut(s) | 497, 1786 |
| BstUI | CGCG | 6 cut(s) | 551, 752, 858, 969, 1069, 1226 |
| BstV1I | GCAGC | 8 cut(s) | 443, 447, 450, 453, 456, 459, 462, 465 |
| BstV2I | GAAGAC | 5 cut(s) | 244, 268, 408, 1279, 1620 |
| BstX2I | RGATCY | 3 cut(s) | 13, 808, 1732 |
| BstYI | RGATCY | 3 cut(s) | 13, 808, 1732 |
| BstZI | CGGCCG | 1 cut(s) | 964 |
| Bsu15I | ATCGAT | 1 cut(s) | 382 |
| BsuRI | GGCC | 9 cut(s) | 5, 497, 590, 745, 966, 1302, 1550, 1589, 1721 |
| BsuTUI | ATCGAT | 1 cut(s) | 382 |
| BtgI | CCRYGG | 3 cut(s) | 20, 259, 1551 |
| BtgZI | GCGATG | 1 cut(s) | 967 |
| BtsCI | GGATG | 6 cut(s) | 687, 721, 931, 1056, 1134, 1404 |
| BtsI | GCAGTG | 1 cut(s) | 219 |
| BtsIMutI | CAGTG | 2 cut(s) | 219, 491 |
| BtuMI | TCGCGA | 1 cut(s) | 1226 |
| BveI | ACCTGC | 2 cut(s) | 556, 1915 |
| Cac8I | GCNNGC | 6 cut(s) | 61, 511, 547, 551, 964, 1446 |
| CaiI | CAGNNNCTG | 1 cut(s) | 456 |
| CfoI | GCGC | 6 cut(s) | 509, 551, 553, 962, 1071, 1109 |
| Cfr10I | RCCGGY | 3 cut(s) | 509, 962, 1531 |
| Cfr13I | GGNCC | 5 cut(s) | 24, 718, 743, 1300, 1548 |
| ClaI | ATCGAT | 1 cut(s) | 382 |
| CseI | GACGC | 3 cut(s) | 74, 1343, 1708 |
| Csp6I | GTAC | 3 cut(s) | 397, 835, 1771 |
| CviAII | CATG | 5 cut(s) | 21, 280, 583, 1318, 1765 |
| CviQI | GTAC | 3 cut(s) | 397, 835, 1771 |
| DdeI | CTNAG | 2 cut(s) | 1173, 1800 |
| DinI | GGCGCC | 1 cut(s) | 961 |
| DraIII | CACNNNGTG | 1 cut(s) | 532 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 977 |
| DriI | GACNNNNNGTC | 1 cut(s) | 413 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 977 |
| EaeI | YGGCCR | 4 cut(s) | 3, 495, 588, 964 |
| EagI | CGGCCG | 1 cut(s) | 964 |
| Eam1104I | CTCTTC | 6 cut(s) | 41, 609, 824, 937, 996, 1474 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 413 |
| EarI | CTCTTC | 6 cut(s) | 41, 609, 824, 937, 996, 1474 |
| EciI | GGCGGA | 6 cut(s) | 20, 194, 274, 624, 768, 1015 |
| Ecl136II | GAGCTC | 2 cut(s) | 165, 334 |
| EclXI | CGGCCG | 1 cut(s) | 964 |
| Eco130I | CCWWGG | 1 cut(s) | 20 |
| Eco24I | GRGCYC | 2 cut(s) | 167, 336 |
| Eco31I | GGTCTC | 1 cut(s) | 1471 |
| Eco32I | GATATC | 1 cut(s) | 386 |
| Eco47I | GGWCC | 2 cut(s) | 24, 718 |
| Eco52I | CGGCCG | 1 cut(s) | 964 |
| Eco53kI | GAGCTC | 2 cut(s) | 165, 334 |
| Eco57I | CTGAAG | 1 cut(s) | 1845 |
| Eco72I | CACGTG | 2 cut(s) | 532, 1259 |
| Eco88I | CYCGRG | 2 cut(s) | 327, 917 |
| EcoICRI | GAGCTC | 2 cut(s) | 165, 334 |
| EcoRI | GAATTC | 1 cut(s) | 1438 |
| EcoRII | CCWGG | 1 cut(s) | 1786 |
| EcoRV | GATATC | 1 cut(s) | 386 |
| EcoT14I | CCWWGG | 1 cut(s) | 20 |
| EcoT38I | GRGCYC | 2 cut(s) | 167, 336 |
| EgeI | GGCGCC | 1 cut(s) | 961 |
| EheI | GGCGCC | 1 cut(s) | 961 |
| ErhI | CCWWGG | 1 cut(s) | 20 |
| Esp3I | CGTCTC | 4 cut(s) | 355, 626, 1123, 1229 |
| FaeI | CATG | 5 cut(s) | 24, 283, 586, 1321, 1768 |
| FaqI | GGGAC | 2 cut(s) | 515, 731 |
| FatI | CATG | 5 cut(s) | 20, 279, 582, 1317, 1764 |
| FauI | CCCGC | 6 cut(s) | 197, 260, 346, 506, 851, 1027 |
| FauNDI | CATATG | 1 cut(s) | 1920 |
| FokI | GGATG | 6 cut(s) | 694, 708, 938, 1043, 1121, 1411 |
| FriOI | GRGCYC | 2 cut(s) | 167, 336 |
| FspBI | CTAG | 1 cut(s) | 1866 |
| GlaI | GCGC | 6 cut(s) | 508, 550, 552, 961, 1070, 1108 |
| HaeII | RGCGCY | 3 cut(s) | 510, 963, 1110 |
| HaeIII | GGCC | 9 cut(s) | 5, 497, 590, 745, 966, 1302, 1550, 1589, 1721 |
| HgaI | GACGC | 3 cut(s) | 74, 1343, 1708 |
| HhaI | GCGC | 6 cut(s) | 509, 551, 553, 962, 1071, 1109 |
| Hin1I | GRCGYC | 6 cut(s) | 66, 171, 414, 960, 1101, 1335 |
| Hin1II | CATG | 5 cut(s) | 24, 283, 586, 1321, 1768 |
| Hin6I | GCGC | 6 cut(s) | 507, 549, 551, 960, 1069, 1107 |
| HinP1I | GCGC | 6 cut(s) | 507, 549, 551, 960, 1069, 1107 |
| HincII | GTYRAC | 3 cut(s) | 94, 317, 358 |
| HindII | GTYRAC | 3 cut(s) | 94, 317, 358 |
| HinfI | GANTC | 9 cut(s) | 408, 475, 626, 929, 1199, 1273, 1358, 1407, 1450 |
| HphI | GGTGA | 7 cut(s) | 79, 169, 518, 670, 1066, 1222, 1708 |
| Hpy166II | GTNNAC | 4 cut(s) | 94, 317, 358, 1480 |
| Hpy8I | GTNNAC | 4 cut(s) | 94, 317, 358, 1480 |
| HpyAV | CCTTC | 5 cut(s) | 195, 682, 1057, 1171, 1339 |
| HpyCH4III | ACNGT | 6 cut(s) | 28, 91, 98, 486, 787, 1555 |
| HpyCH4IV | ACGT | 5 cut(s) | 171, 414, 531, 1101, 1258 |
| HpyCH4V | TGCA | 5 cut(s) | 817, 1035, 1248, 1826, 1924 |
| HpyF3I | CTNAG | 2 cut(s) | 1173, 1800 |
| HpySE526I | ACGT | 5 cut(s) | 171, 414, 531, 1101, 1258 |
| Hsp92I | GRCGYC | 6 cut(s) | 66, 171, 414, 960, 1101, 1335 |
| Hsp92II | CATG | 5 cut(s) | 24, 283, 586, 1321, 1768 |
| HspAI | GCGC | 6 cut(s) | 507, 549, 551, 960, 1069, 1107 |
| KasI | GGCGCC | 1 cut(s) | 959 |
| KroI | GCCGGC | 2 cut(s) | 509, 962 |
| KroNI | GCCGGC | 2 cut(s) | 511, 964 |
| LguI | GCTCTTC | 1 cut(s) | 609 |
| Lsp1109I | GCAGC | 8 cut(s) | 443, 447, 450, 453, 456, 459, 462, 465 |
| LweI | GCATC | 1 cut(s) | 388 |
| MaeI | CTAG | 1 cut(s) | 1866 |
| MaeII | ACGT | 5 cut(s) | 171, 414, 531, 1101, 1258 |
| MaeIII | GTNAC | 4 cut(s) | 175, 1270, 1696, 1891 |
| MbiI | CCGCTC | 3 cut(s) | 619, 670, 1062 |
| MfeI | CAATTG | 1 cut(s) | 1459 |
| MflI | RGATCY | 3 cut(s) | 13, 808, 1732 |
| MhlI | GDGCHC | 4 cut(s) | 167, 336, 1507, 1713 |
| MlsI | TGGCCA | 2 cut(s) | 5, 590 |
| MluCI | AATT | 3 cut(s) | 1438, 1459, 1827 |
| MluNI | TGGCCA | 2 cut(s) | 5, 590 |
| Mly113I | GGCGCC | 1 cut(s) | 960 |
| MlyI | GAGTC | 5 cut(s) | 402, 923, 1267, 1367, 1401 |
| MmeI | TCCRAC | 6 cut(s) | 192, 690, 903, 1191, 1356, 1660 |
| Mox20I | TGGCCA | 2 cut(s) | 5, 590 |
| MreI | CGCCGGCG | 1 cut(s) | 509 |
| MroNI | GCCGGC | 2 cut(s) | 509, 962 |
| MroXI | GAANNNNTTC | 2 cut(s) | 113, 737 |
| MscI | TGGCCA | 2 cut(s) | 5, 590 |
| MseI | TTAA | 2 cut(s) | 137, 1578 |
| Msp20I | TGGCCA | 2 cut(s) | 5, 590 |
| MspA1I | CMGCKG | 2 cut(s) | 456, 655 |
| MspR9I | CCNGG | 2 cut(s) | 499, 1788 |
| MunI | CAATTG | 1 cut(s) | 1459 |
| Mva1269I | GAATGC | 2 cut(s) | 895, 1248 |
| MvaI | CCWGG | 1 cut(s) | 1788 |
| MvnI | CGCG | 6 cut(s) | 551, 752, 858, 969, 1069, 1226 |
| NaeI | GCCGGC | 2 cut(s) | 511, 964 |
| NarI | GGCGCC | 1 cut(s) | 960 |
| NciI | CCSGG | 1 cut(s) | 499 |
| NcoI | CCATGG | 1 cut(s) | 20 |
| NdeI | CATATG | 1 cut(s) | 1920 |
| NgoMIV | GCCGGC | 2 cut(s) | 509, 962 |
| NlaIII | CATG | 5 cut(s) | 24, 283, 586, 1321, 1768 |
| NlaIV | GGNNCC | 7 cut(s) | 203, 719, 810, 961, 1301, 1597, 1734 |
| NmeAIII | GCCGAG | 1 cut(s) | 1693 |
| NmuCI | GTSAC | 3 cut(s) | 175, 1270, 1696 |
| NruI | TCGCGA | 1 cut(s) | 1226 |
| NspI | RCATGY | 3 cut(s) | 586, 1321, 1768 |
| NspV | TTCGAA | 1 cut(s) | 1343 |
| PauI | GCGCGC | 1 cut(s) | 549 |
| PciI | ACATGT | 1 cut(s) | 1764 |
| PciSI | GCTCTTC | 1 cut(s) | 609 |
| PcsI | WCGNNNNNNNCGW | 7 cut(s) | 177, 351, 411, 1005, 1230, 1329, 1559 |
| PctI | GAATGC | 2 cut(s) | 895, 1248 |
| PdiI | GCCGGC | 2 cut(s) | 511, 964 |
| PdmI | GAANNNNTTC | 2 cut(s) | 113, 737 |
| PfeI | GAWTC | 4 cut(s) | 475, 626, 1199, 1450 |
| PflFI | GACNNNGTC | 2 cut(s) | 173, 1484 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 727 |
| PleI | GAGTC | 5 cut(s) | 402, 923, 1267, 1366, 1401 |
| PluTI | GGCGCC | 1 cut(s) | 963 |
| PmaCI | CACGTG | 2 cut(s) | 532, 1259 |
| PmlI | CACGTG | 2 cut(s) | 532, 1259 |
| PpsI | GAGTC | 5 cut(s) | 402, 923, 1267, 1366, 1401 |
| Ppu21I | YACGTR | 2 cut(s) | 532, 1259 |
| PscI | ACATGT | 1 cut(s) | 1764 |
| Psp124BI | GAGCTC | 2 cut(s) | 167, 336 |
| Psp6I | CCWGG | 1 cut(s) | 1786 |
| PspCI | CACGTG | 2 cut(s) | 532, 1259 |
| PspGI | CCWGG | 1 cut(s) | 1786 |
| PspN4I | GGNNCC | 7 cut(s) | 203, 719, 810, 961, 1301, 1597, 1734 |
| PspPI | GGNCC | 5 cut(s) | 24, 718, 743, 1300, 1548 |
| PstNI | CAGNNNCTG | 1 cut(s) | 456 |
| PsuI | RGATCY | 3 cut(s) | 13, 808, 1732 |
| PsyI | GACNNNGTC | 2 cut(s) | 173, 1484 |
| PteI | GCGCGC | 1 cut(s) | 549 |
| PvuII | CAGCTG | 1 cut(s) | 456 |
| RruI | TCGCGA | 1 cut(s) | 1226 |
| RsaI | GTAC | 3 cut(s) | 398, 836, 1772 |
| RsaNI | GTAC | 3 cut(s) | 397, 835, 1771 |
| SacI | GAGCTC | 2 cut(s) | 167, 336 |
| SapI | GCTCTTC | 1 cut(s) | 609 |
| SaqAI | TTAA | 2 cut(s) | 137, 1578 |
| Sau96I | GGNCC | 5 cut(s) | 24, 718, 743, 1300, 1548 |
| SchI | GAGTC | 5 cut(s) | 402, 923, 1267, 1367, 1401 |
| ScrFI | CCNGG | 2 cut(s) | 499, 1788 |
| SduI | GDGCHC | 4 cut(s) | 167, 336, 1507, 1713 |
| SfaNI | GCATC | 1 cut(s) | 388 |
| SfoI | GGCGCC | 1 cut(s) | 961 |
| SfuI | TTCGAA | 1 cut(s) | 1343 |
| SgrAI | CRCCGGYG | 1 cut(s) | 509 |
| SinI | GGWCC | 2 cut(s) | 24, 718 |
| SmlI | CTYRAG | 3 cut(s) | 158, 419, 1937 |
| SmoI | CTYRAG | 3 cut(s) | 158, 419, 1937 |
| Sse9I | AATT | 3 cut(s) | 1438, 1459, 1827 |
| SspDI | GGCGCC | 1 cut(s) | 959 |
| SspMI | CTAG | 1 cut(s) | 1866 |
| SstI | GAGCTC | 2 cut(s) | 167, 336 |
| StyD4I | CCNGG | 2 cut(s) | 497, 1786 |
| StyI | CCWWGG | 1 cut(s) | 20 |
| TaaI | ACNGT | 6 cut(s) | 28, 91, 98, 486, 787, 1555 |
| TaiI | ACGT | 5 cut(s) | 174, 417, 534, 1104, 1261 |
| TasI | AATT | 3 cut(s) | 1438, 1459, 1827 |
| TatI | WGTACW | 1 cut(s) | 1770 |
| TauI | GCSGC | 7 cut(s) | 227, 267, 655, 969, 1112, 1115, 1133 |
| TfiI | GAWTC | 4 cut(s) | 475, 626, 1199, 1450 |
| Tru1I | TTAA | 2 cut(s) | 137, 1578 |
| Tru9I | TTAA | 2 cut(s) | 137, 1578 |
| TscAI | CASTG | 2 cut(s) | 226, 491 |
| TseFI | GTSAC | 3 cut(s) | 175, 1270, 1696 |
| TseI | GCWGC | 8 cut(s) | 435, 438, 441, 444, 447, 450, 453, 456 |
| Tsp45I | GTSAC | 3 cut(s) | 175, 1270, 1696 |
| TspDTI | ATGAA | 4 cut(s) | 268, 415, 1892, 1907 |
| TspGWI | ACGGA | 8 cut(s) | 34, 343, 378, 420, 638, 689, 801, 967 |
| TspRI | CASTG | 2 cut(s) | 226, 491 |
| Tth111I | GACNNNGTC | 2 cut(s) | 173, 1484 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 727 |
| VpaK11BI | GGWCC | 2 cut(s) | 24, 718 |
| XapI | RAATTY | 1 cut(s) | 1438 |
| XceI | RCATGY | 3 cut(s) | 586, 1321, 1768 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 18 |
| XmnI | GAANNNNTTC | 2 cut(s) | 113, 737 |
| XspI | CTAG | 1 cut(s) | 1866 |
| ZraI | GACGTC | 3 cut(s) | 172, 415, 1102 |