RLG00000008787

Endochitinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0001782
Physical Location & Seq
Forward (+)
39197832 .. 39198878
1047 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008787

Sequence Viewer

Length: 828 bp
ATGGCTTTCCAAGTTACAATTAGGACAAAATCCCTTTTCTTCACCATCCTAGGCTTAGCATTTGTAGCCCAAATTGCAATAGCTCAAGACTGCGGGTGTGCCGCGGGCCTGTGTTGCAGCGAATTTGGTTACTGCGGCACCGGCGATCCCTACTGCGGCAAGGGTTGTAAAGAAGGTCCTTGTACTACCACCCCCACACCAACACCAACTACTAATGGAGGCTCGGTGGCAGATATCGTGACCACTGACTTCTTTAACGGGATTATTAACCAGGCTGGCGCTAACTGTGCCGGAAAGAGCTTCTACACCAGACAGGCTTTTCTCGATGCCCTCGGCTCTTATGGTGATTTCGGTACCACCGGTTCCGCTGATGACAATAAGCGGGAAGTTGCAGCGCTGTTTGCTCATGTCACGCATGAGACTGGCTTTTTTTGCTTCATTGAAGAGGAAAATAAGGACACCTACTGCGACACGAGCAGAACAGACTACCCATGCAACCCCAACAAGCAGTACTACGGGCGTGGACCACTCCAGCTCACCTGGAACTACAATTACGGAGCTGCCGGAAATGCCATTGGCTTTGACGGCTTGAACTCCCCCGAAACGGTGGCTCAAGACCCCGTGATAGCATTCAAGACAGCCTTGTGGTATTGGATGACCAATGTTCACTCCGTTATAGGCCAAGGATTCGGAGCCACAACTCGAGCCATCAACGGTGCTGTGGAATGTGATGGCAAATCCCCCGCCACAGTTCAAGCTCGCGCCAACTATTACACTAACTACTGCACCCAATTTAATGTTGCTCCCGGTGACAATCTCTTGTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00520 ko01100 map00520 map01100
Pfam Domains
Protein Families
Chitin_bind_1 Glyco_hydro_19

Protein Analysis

276

Amino Acids

29.42

Weight (kDa)

4.79

Isoelectric Point (pI)

23.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Chitin_bind_1 PF00187 32 - 61 6.8e-07 Chitin recognition protein
Glyco_hydro_19 PF00182 79 - 275 2.2e-46 Chitinase class I
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 353
AccB1I GGYRCC 2 cut(s) 137, 353
AccII CGCG 2 cut(s) 104, 762
AciI CCGC 8 cut(s) 93, 102, 104, 135, 156, 366, 382, 744
AclWI GGATC 1 cut(s) 140
AcsI RAATTY 1 cut(s) 122
AfaI GTAC 3 cut(s) 184, 355, 512
AfeI AGCGCT 1 cut(s) 396
AfiI CCNNNNNNNGG 2 cut(s) 155, 604
AgeI ACCGGT 1 cut(s) 359
AgsI TTSAA 4 cut(s) 443, 592, 634, 755
AjnI CCWGG 2 cut(s) 270, 539
AluBI AGCT 5 cut(s) 83, 300, 535, 560, 758
AluI AGCT 5 cut(s) 83, 300, 535, 560, 758
Alw26I GTCTC 1 cut(s) 413
AlwI GGATC 1 cut(s) 140
Ama87I CYCGRG 1 cut(s) 702
Aor51HI AGCGCT 1 cut(s) 396
AoxI GGCC 2 cut(s) 106, 679
ApeKI GCWGC 3 cut(s) 117, 392, 560
ApoI RAATTY 1 cut(s) 122
AsiGI ACCGGT 1 cut(s) 359
Asp718I GGTACC 1 cut(s) 353
AspA2I CCTAGG 1 cut(s) 49
AspLEI GCGC 3 cut(s) 281, 397, 764
AspS9I GGNCC 3 cut(s) 106, 176, 524
AsuC2I CCSGG 1 cut(s) 807
AsuHPI GGTGA 4 cut(s) 34, 356, 529, 821
AvaI CYCGRG 1 cut(s) 702
AvaII GGWCC 2 cut(s) 176, 524
AvrII CCTAGG 1 cut(s) 49
BanI GGYRCC 2 cut(s) 137, 353
BauI CACGAG 1 cut(s) 472
BbvI GCAGC 3 cut(s) 129, 404, 547
BccI CCATC 3 cut(s) 53, 716, 725
BceAI ACGGC 1 cut(s) 601
BciT130I CCWGG 2 cut(s) 272, 541
BcnI CCSGG 1 cut(s) 807
BcoDI GTCTC 1 cut(s) 413
BfaI CTAG 1 cut(s) 50
BfoI RGCGCY 2 cut(s) 282, 398
BisI GCNGC 6 cut(s) 102, 118, 136, 157, 393, 561
BlnI CCTAGG 1 cut(s) 49
BlpI GCTNAGC 1 cut(s) 55
BlsI GCNGC 6 cut(s) 103, 119, 137, 158, 394, 562
BmcAI AGTACT 1 cut(s) 512
Bme1390I CCNGG 3 cut(s) 272, 541, 807
Bme18I GGWCC 2 cut(s) 176, 524
BmeT110I CYCGRG 1 cut(s) 702
BmgT120I GGNCC 3 cut(s) 106, 176, 524
BmiI GGNNCC 4 cut(s) 139, 355, 364, 694
BmrFI CCNGG 3 cut(s) 272, 541, 807
BmsI GCATC 1 cut(s) 316
BpmI CTGGAG 1 cut(s) 515
Bpu1102I GCTNAGC 1 cut(s) 55
BpuEI CTTGAG 2 cut(s) 69, 597
BpuMI CCSGG 1 cut(s) 807
BsaJI CCNNGG 4 cut(s) 49, 102, 331, 682
BsaWI WCCGGW 1 cut(s) 359
BsaXI ACNNNNNCTCC 2 cut(s) 210, 240
Bsc4I CCNNNNNNNGG 2 cut(s) 155, 604
Bse118I RCCGGY 2 cut(s) 140, 359
Bse1I ACTGG 1 cut(s) 427
BseBI CCWGG 2 cut(s) 272, 541
BseDI CCNNGG 4 cut(s) 49, 102, 331, 682
BseGI GGATG 2 cut(s) 45, 660
BseLI CCNNNNNNNGG 2 cut(s) 155, 604
BseNI ACTGG 1 cut(s) 427
BseXI GCAGC 3 cut(s) 129, 404, 547
BsgI GTGCAG 1 cut(s) 769
Bsh1236I CGCG 2 cut(s) 104, 762
BshFI GGCC 2 cut(s) 108, 681
BshNI GGYRCC 2 cut(s) 137, 353
BshTI ACCGGT 1 cut(s) 359
BsiHKCI CYCGRG 1 cut(s) 702
BsiSI CCGG 5 cut(s) 141, 291, 360, 564, 807
BslI CCNNNNNNNGG 2 cut(s) 155, 604
BsmAI GTCTC 1 cut(s) 413
BsmI GAATGC 1 cut(s) 629
BsnI GGCC 2 cut(s) 108, 681
BsoBI CYCGRG 1 cut(s) 702
Bsp143I GATC 1 cut(s) 145
Bsp1720I GCTNAGC 1 cut(s) 55
BspACI CCGC 8 cut(s) 93, 102, 104, 135, 156, 366, 382, 744
BspANI GGCC 2 cut(s) 108, 681
BspFNI CGCG 2 cut(s) 104, 762
BspLI GGNNCC 4 cut(s) 139, 355, 364, 694
BspPI GGATC 1 cut(s) 140
BspT107I GGYRCC 2 cut(s) 137, 353
BsrFI RCCGGY 2 cut(s) 140, 359
BsrI ACTGG 1 cut(s) 427
BssAI RCCGGY 2 cut(s) 140, 359
BssECI CCNNGG 4 cut(s) 49, 102, 331, 682
BssMI GATC 1 cut(s) 145
BssSI CACGAG 1 cut(s) 472
BssT1I CCWWGG 2 cut(s) 49, 682
Bst2BI CACGAG 1 cut(s) 472
Bst2UI CCWGG 2 cut(s) 272, 541
Bst4CI ACNGT 4 cut(s) 287, 607, 716, 751
Bst6I CTCTTC 1 cut(s) 438
BstC8I GCNNGC 3 cut(s) 106, 277, 760
BstDEI CTNAG 1 cut(s) 55
BstDSI CCRYGG 1 cut(s) 102
BstF5I GGATG 2 cut(s) 45, 660
BstFNI CGCG 2 cut(s) 104, 762
BstH2I RGCGCY 2 cut(s) 282, 398
BstHHI GCGC 3 cut(s) 281, 397, 764
BstKTI GATC 1 cut(s) 148
BstMAI GTCTC 1 cut(s) 413
BstMBI GATC 1 cut(s) 145
BstNI CCWGG 2 cut(s) 272, 541
BstSCI CCNGG 3 cut(s) 270, 539, 805
BstUI CGCG 2 cut(s) 104, 762
BstV1I GCAGC 3 cut(s) 129, 404, 547
BsuRI GGCC 2 cut(s) 108, 681
BtgI CCRYGG 1 cut(s) 102
BtsCI GGATG 2 cut(s) 45, 660
BtsIMutI CAGTG 1 cut(s) 243
Cac8I GCNNGC 3 cut(s) 106, 277, 760
CfoI GCGC 3 cut(s) 281, 397, 764
Cfr10I RCCGGY 2 cut(s) 140, 359
Cfr13I GGNCC 3 cut(s) 106, 176, 524
Cfr42I CCGCGG 1 cut(s) 105
Csp6I GTAC 3 cut(s) 183, 354, 511
CspAI ACCGGT 1 cut(s) 359
CviAII CATG 3 cut(s) 407, 416, 492
CviQI GTAC 3 cut(s) 183, 354, 511
DdeI CTNAG 1 cut(s) 55
DpnI GATC 1 cut(s) 147
DpnII GATC 1 cut(s) 145
Eam1104I CTCTTC 1 cut(s) 438
EarI CTCTTC 1 cut(s) 438
Eco130I CCWWGG 2 cut(s) 49, 682
Eco32I GATATC 1 cut(s) 235
Eco47I GGWCC 2 cut(s) 176, 524
Eco47III AGCGCT 1 cut(s) 396
Eco88I CYCGRG 1 cut(s) 702
EcoO109I RGGNCCY 1 cut(s) 176
EcoRII CCWGG 2 cut(s) 270, 539
EcoRV GATATC 1 cut(s) 235
EcoT14I CCWWGG 2 cut(s) 49, 682
ErhI CCWWGG 2 cut(s) 49, 682
FaeI CATG 3 cut(s) 410, 419, 495
FaiI YATR 5 cut(s) 342, 408, 417, 493, 677
FalI AAGNNNNNCTT 2 cut(s) 626, 658
FatI CATG 3 cut(s) 406, 415, 491
FauI CCCGC 4 cut(s) 86, 97, 375, 751
Fnu4HI GCNGC 6 cut(s) 102, 118, 136, 157, 393, 561
FokI GGATG 2 cut(s) 32, 667
Fsp4HI GCNGC 6 cut(s) 102, 118, 136, 157, 393, 561
FspBI CTAG 1 cut(s) 50
GlaI GCGC 3 cut(s) 280, 396, 763
GluI GCNGC 6 cut(s) 102, 118, 136, 157, 393, 561
GsuI CTGGAG 1 cut(s) 515
HaeII RGCGCY 2 cut(s) 282, 398
HaeIII GGCC 2 cut(s) 108, 681
HapII CCGG 5 cut(s) 141, 291, 360, 564, 807
HhaI GCGC 3 cut(s) 281, 397, 764
Hin1II CATG 3 cut(s) 410, 419, 495
Hin6I GCGC 3 cut(s) 279, 395, 762
HinP1I GCGC 3 cut(s) 279, 395, 762
HinfI GANTC 1 cut(s) 687
HpaII CCGG 5 cut(s) 141, 291, 360, 564, 807
HphI GGTGA 4 cut(s) 34, 356, 529, 821
Hpy166II GTNNAC 2 cut(s) 524, 667
Hpy188I TCNGA 1 cut(s) 692
Hpy188III TCNNGA 5 cut(s) 86, 238, 323, 614, 634
Hpy8I GTNNAC 2 cut(s) 524, 667
HpyAV CCTTC 1 cut(s) 167
HpyCH4III ACNGT 4 cut(s) 287, 607, 716, 751
HpyCH4V TGCA 5 cut(s) 77, 117, 392, 495, 786
HpyF3I CTNAG 1 cut(s) 55
Hsp92II CATG 3 cut(s) 410, 419, 495
HspAI GCGC 3 cut(s) 279, 395, 762
KpnI GGTACC 1 cut(s) 357
KspI CCGCGG 1 cut(s) 105
Kzo9I GATC 1 cut(s) 145
LmnI GCTCC 3 cut(s) 557, 692, 808
Lsp1109I GCAGC 3 cut(s) 129, 404, 547
LweI GCATC 1 cut(s) 316
MaeI CTAG 1 cut(s) 50
MaeIII GTNAC 5 cut(s) 13, 128, 238, 409, 809
MalI GATC 1 cut(s) 147
MboI GATC 1 cut(s) 145
MboII GAAGA 2 cut(s) 31, 455
MluCI AATT 5 cut(s) 18, 72, 122, 550, 791
MnlI CCTC 3 cut(s) 212, 341, 439
MseI TTAA 3 cut(s) 255, 267, 795
MspA1I CMGCKG 2 cut(s) 104, 368
MspI CCGG 5 cut(s) 141, 291, 360, 564, 807
MspR9I CCNGG 3 cut(s) 272, 541, 807
Mva1269I GAATGC 1 cut(s) 629
MvaI CCWGG 2 cut(s) 272, 541
MvnI CGCG 2 cut(s) 104, 762
NciI CCSGG 1 cut(s) 807
NdeII GATC 1 cut(s) 145
NlaIII CATG 3 cut(s) 410, 419, 495
NlaIV GGNNCC 4 cut(s) 139, 355, 364, 694
NmeAIII GCCGAG 1 cut(s) 312
NmuCI GTSAC 3 cut(s) 238, 409, 809
PaeR7I CTCGAG 1 cut(s) 702
PctI GAATGC 1 cut(s) 629
PfeI GAWTC 1 cut(s) 687
PinAI ACCGGT 1 cut(s) 359
PkrI GCNGC 6 cut(s) 103, 119, 137, 158, 394, 562
PpuMI RGGWCCY 1 cut(s) 176
Psp5II RGGWCCY 1 cut(s) 176
Psp6I CCWGG 2 cut(s) 270, 539
PspGI CCWGG 2 cut(s) 270, 539
PspN4I GGNNCC 4 cut(s) 139, 355, 364, 694
PspPI GGNCC 3 cut(s) 106, 176, 524
PspPPI RGGWCCY 1 cut(s) 176
PspXI VCTCGAGB 1 cut(s) 702
PsrI GAACNNNNNNTAC 4 cut(s) 346, 378, 536, 568
RsaI GTAC 3 cut(s) 184, 355, 512
RsaNI GTAC 3 cut(s) 183, 354, 511
SacII CCGCGG 1 cut(s) 105
SaqAI TTAA 3 cut(s) 255, 267, 795
SatI GCNGC 6 cut(s) 102, 118, 136, 157, 393, 561
Sau3AI GATC 1 cut(s) 145
Sau96I GGNCC 3 cut(s) 106, 176, 524
ScaI AGTACT 1 cut(s) 512
ScrFI CCNGG 3 cut(s) 272, 541, 807
SetI ASST 8 cut(s) 85, 178, 302, 464, 537, 542, 562, 760
SfaNI GCATC 1 cut(s) 316
Sfr274I CTCGAG 1 cut(s) 702
Sfr303I CCGCGG 1 cut(s) 105
SgrAI CRCCGGYG 1 cut(s) 140
SgrBI CCGCGG 1 cut(s) 105
SinI GGWCC 2 cut(s) 176, 524
SlaI CTCGAG 1 cut(s) 702
SmlI CTYRAG 3 cut(s) 84, 612, 702
SmoI CTYRAG 3 cut(s) 84, 612, 702
Sse9I AATT 5 cut(s) 18, 72, 122, 550, 791
SsiI CCGC 8 cut(s) 93, 102, 104, 135, 156, 366, 382, 744
SspMI CTAG 1 cut(s) 50
StyD4I CCNGG 3 cut(s) 270, 539, 805
StyI CCWWGG 2 cut(s) 49, 682
TaaI ACNGT 4 cut(s) 287, 607, 716, 751
TaqI TCGA 2 cut(s) 324, 703
TasI AATT 5 cut(s) 18, 72, 122, 550, 791
TatI WGTACW 2 cut(s) 182, 510
TauI GCSGC 3 cut(s) 104, 138, 159
TfiI GAWTC 1 cut(s) 687
Tru1I TTAA 3 cut(s) 255, 267, 795
Tru9I TTAA 3 cut(s) 255, 267, 795
TscAI CASTG 1 cut(s) 250
TseFI GTSAC 3 cut(s) 238, 409, 809
TseI GCWGC 3 cut(s) 117, 392, 560
Tsp45I GTSAC 3 cut(s) 238, 409, 809
TspDTI ATGAA 1 cut(s) 427
TspGWI ACGGA 2 cut(s) 570, 661
TspRI CASTG 1 cut(s) 250
VpaK11BI GGWCC 2 cut(s) 176, 524
XapI RAATTY 1 cut(s) 122
XhoI CTCGAG 1 cut(s) 702
XmaJI CCTAGG 1 cut(s) 49
XspI CTAG 1 cut(s) 50
ZrmI AGTACT 1 cut(s) 512
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.