Rh4BG133900

Endochitinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0001782
Physical Location & Seq
Reverse (-)
22506226 .. 22507542
1317 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG133900.1

Sequence Viewer

Length: 828 bp
ATGGCTTTCCAAGTTACAATTAGGACGAAGTCCCTTTTCTTCACCATCCTAGGCTTAGCATTTGTAGCCCAAATTGCAATAGCTCAAGACTGCGGATGTGCCGCAGGCCTGTGTTGCAGCGAATTTGGTTACTGCGGCACCGGCGATGCCTACTGCGGCAAGGGTTGTAAAGAAGGTCCTTGTACTACCACCCCCACACCGACACCAACTACTAACGGTGGCTCGGTGGCAGATATTGTGACCACTGACTTCTTTAACGGGATTATTAATCAGGCTGGTGCTGACTGTGCCGGAAAGAGCTTCTACACCAGACAGTCTTTTCTCGATGCCCTCGGCTCTTATGGTGATTTCGGTACCACCGGTTCCGCTGATGACAATAAGCGGGAAGTTGCAGCGCTGTTTGCTCATGTCACGCATGAGACTGGCCATTTTTGCTACATTGAAGAGATAAATAAGAACACCTACTGCGACGGGAGCAGAACAGACTACCCATGCAACCCCAGCAAGCAGTACTACGGGCGTGGACCACTCCAGCTCACCTGGAACTACAATTACGGAGCTGCCGGAAATGCCATTGGCTTTGACGGCCTGAACTCCCCCGAAACGGTGGCTACAGACCCCGTGGTGGCGTTCAAAACGGGCTTGTGGTTTTGGATGACCAATGTGCACTCCGTTATAGGCCAAGGATTTGGAGCCACAACTCGAGCCATCAACGGTGACGTGGAATGTGATGGCAAAGCTCCGGACAAAGTTCAAGCTCGCGTCAACTATTATAATGACTACTGCACCCAACTCAATGTTTCTCCGGGTGACAATCTCTATTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00520 ko01100 map00520 map01100
Pfam Domains
Protein Families
Chitin_bind_1 Glyco_hydro_19

Protein Analysis

275

Amino Acids

29.43

Weight (kDa)

4.8

Isoelectric Point (pI)

24.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Chitin_bind_1 PF00187 32 - 61 2.3e-07 Chitin recognition protein
Glyco_hydro_19 PF00182 79 - 222 4.7e-40 Chitinase class I
Glyco_hydro_19 PF00182 229 - 275 4.3e-09 Chitinase class I
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 774
Acc65I GGTACC 1 cut(s) 353
AccB1I GGYRCC 2 cut(s) 137, 353
AccII CGCG 1 cut(s) 762
AccIII TCCGGA 1 cut(s) 742
AciI CCGC 6 cut(s) 93, 102, 135, 156, 366, 382
AcoI YGGCCR 1 cut(s) 424
AcsI RAATTY 1 cut(s) 122
AfaI GTAC 3 cut(s) 184, 355, 512
AfeI AGCGCT 1 cut(s) 396
AfiI CCNNNNNNNGG 2 cut(s) 604, 625
AgeI ACCGGT 1 cut(s) 359
AgsI TTSAA 3 cut(s) 443, 634, 755
AjiI CACGTC 1 cut(s) 721
AjnI CCWGG 1 cut(s) 539
AluBI AGCT 6 cut(s) 83, 300, 535, 560, 740, 758
AluI AGCT 6 cut(s) 83, 300, 535, 560, 740, 758
Alw21I GWGCWC 1 cut(s) 669
Alw26I GTCTC 1 cut(s) 413
Alw44I GTGCAC 1 cut(s) 665
Ama87I CYCGRG 1 cut(s) 702
Aor13HI TCCGGA 1 cut(s) 742
Aor51HI AGCGCT 1 cut(s) 396
AoxI GGCC 4 cut(s) 106, 424, 586, 679
ApaLI GTGCAC 1 cut(s) 665
ApeKI GCWGC 3 cut(s) 117, 392, 560
ApoI RAATTY 1 cut(s) 122
AseI ATTAAT 1 cut(s) 267
AsiGI ACCGGT 1 cut(s) 359
Asp718I GGTACC 1 cut(s) 353
AspA2I CCTAGG 1 cut(s) 49
AspLEI GCGC 1 cut(s) 397
AspS9I GGNCC 2 cut(s) 176, 524
AsuC2I CCSGG 1 cut(s) 807
AsuHPI GGTGA 5 cut(s) 34, 356, 529, 728, 821
AvaI CYCGRG 1 cut(s) 702
AvaII GGWCC 2 cut(s) 176, 524
AvrII CCTAGG 1 cut(s) 49
BaeGI GKGCMC 1 cut(s) 669
BalI TGGCCA 1 cut(s) 426
BanI GGYRCC 2 cut(s) 137, 353
Bbv12I GWGCWC 1 cut(s) 669
BbvI GCAGC 3 cut(s) 129, 404, 547
BccI CCATC 3 cut(s) 53, 716, 725
BceAI ACGGC 1 cut(s) 601
BciT130I CCWGG 1 cut(s) 541
BcnI CCSGG 1 cut(s) 807
BcoDI GTCTC 1 cut(s) 413
BfaI CTAG 1 cut(s) 50
BfmI CTRYAG 1 cut(s) 612
BfoI RGCGCY 1 cut(s) 398
BisI GCNGC 6 cut(s) 102, 118, 136, 157, 393, 561
BlnI CCTAGG 1 cut(s) 49
BlpI GCTNAGC 1 cut(s) 55
BlsI GCNGC 6 cut(s) 103, 119, 137, 158, 394, 562
BmcAI AGTACT 1 cut(s) 512
Bme1390I CCNGG 2 cut(s) 541, 807
Bme18I GGWCC 2 cut(s) 176, 524
BmeT110I CYCGRG 1 cut(s) 702
BmgBI CACGTC 1 cut(s) 721
BmgT120I GGNCC 2 cut(s) 176, 524
BmiI GGNNCC 4 cut(s) 139, 355, 364, 694
BmrFI CCNGG 2 cut(s) 541, 807
BmsI GCATC 2 cut(s) 136, 316
BpmI CTGGAG 1 cut(s) 515
Bpu1102I GCTNAGC 1 cut(s) 55
BpuEI CTTGAG 1 cut(s) 69
BpuMI CCSGG 1 cut(s) 807
BsaJI CCNNGG 4 cut(s) 49, 331, 621, 682
BsaWI WCCGGW 2 cut(s) 359, 742
Bsc4I CCNNNNNNNGG 2 cut(s) 604, 625
Bse118I RCCGGY 2 cut(s) 140, 359
Bse1I ACTGG 1 cut(s) 427
BseAI TCCGGA 1 cut(s) 742
BseBI CCWGG 1 cut(s) 541
BseDI CCNNGG 4 cut(s) 49, 331, 621, 682
BseGI GGATG 3 cut(s) 45, 101, 660
BseLI CCNNNNNNNGG 2 cut(s) 604, 625
BseNI ACTGG 1 cut(s) 427
BseSI GKGCMC 1 cut(s) 669
BseXI GCAGC 3 cut(s) 129, 404, 547
BseYI CCCAGC 1 cut(s) 500
BsgI GTGCAG 1 cut(s) 769
Bsh1236I CGCG 1 cut(s) 762
BshFI GGCC 4 cut(s) 108, 426, 588, 681
BshNI GGYRCC 2 cut(s) 137, 353
BshTI ACCGGT 1 cut(s) 359
BsiHKAI GWGCWC 1 cut(s) 669
BsiHKCI CYCGRG 1 cut(s) 702
BsiSI CCGG 6 cut(s) 141, 291, 360, 564, 743, 806
BslFI GGGAC 1 cut(s) 16
BslI CCNNNNNNNGG 2 cut(s) 604, 625
BsmAI GTCTC 1 cut(s) 413
BsmFI GGGAC 1 cut(s) 16
BsnI GGCC 4 cut(s) 108, 426, 588, 681
BsoBI CYCGRG 1 cut(s) 702
Bsp1286I GDGCHC 1 cut(s) 669
Bsp13I TCCGGA 1 cut(s) 742
Bsp1720I GCTNAGC 1 cut(s) 55
BspACI CCGC 6 cut(s) 93, 102, 135, 156, 366, 382
BspANI GGCC 4 cut(s) 108, 426, 588, 681
BspEI TCCGGA 1 cut(s) 742
BspFNI CGCG 1 cut(s) 762
BspLI GGNNCC 4 cut(s) 139, 355, 364, 694
BspT107I GGYRCC 2 cut(s) 137, 353
BsrFI RCCGGY 2 cut(s) 140, 359
BsrI ACTGG 1 cut(s) 427
BssAI RCCGGY 2 cut(s) 140, 359
BssECI CCNNGG 4 cut(s) 49, 331, 621, 682
BssT1I CCWWGG 2 cut(s) 49, 682
Bst2UI CCWGG 1 cut(s) 541
Bst4CI ACNGT 5 cut(s) 218, 287, 315, 607, 716
Bst6I CTCTTC 1 cut(s) 438
BstC8I GCNNGC 3 cut(s) 106, 506, 760
BstDEI CTNAG 1 cut(s) 55
BstDSI CCRYGG 1 cut(s) 621
BstF5I GGATG 3 cut(s) 45, 101, 660
BstFNI CGCG 1 cut(s) 762
BstH2I RGCGCY 1 cut(s) 398
BstHHI GCGC 1 cut(s) 397
BstMAI GTCTC 1 cut(s) 413
BstNI CCWGG 1 cut(s) 541
BstSCI CCNGG 2 cut(s) 539, 805
BstSFI CTRYAG 1 cut(s) 612
BstSLI GKGCMC 1 cut(s) 669
BstUI CGCG 1 cut(s) 762
BstV1I GCAGC 3 cut(s) 129, 404, 547
BstXI CCANNNNNNTGG 1 cut(s) 689
BsuRI GGCC 4 cut(s) 108, 426, 588, 681
BtgI CCRYGG 1 cut(s) 621
BtgZI GCGATG 1 cut(s) 159
BtrI CACGTC 1 cut(s) 721
BtsCI GGATG 3 cut(s) 45, 101, 660
BtsIMutI CAGTG 1 cut(s) 243
Cac8I GCNNGC 3 cut(s) 106, 506, 760
CfoI GCGC 1 cut(s) 397
Cfr10I RCCGGY 2 cut(s) 140, 359
Cfr13I GGNCC 2 cut(s) 176, 524
CseI GACGC 1 cut(s) 751
Csp6I GTAC 3 cut(s) 183, 354, 511
CspAI ACCGGT 1 cut(s) 359
CviAII CATG 3 cut(s) 407, 416, 492
CviQI GTAC 3 cut(s) 183, 354, 511
DdeI CTNAG 1 cut(s) 55
EaeI YGGCCR 1 cut(s) 424
Eam1104I CTCTTC 1 cut(s) 438
EarI CTCTTC 1 cut(s) 438
Eco130I CCWWGG 2 cut(s) 49, 682
Eco147I AGGCCT 1 cut(s) 108
Eco47I GGWCC 2 cut(s) 176, 524
Eco47III AGCGCT 1 cut(s) 396
Eco88I CYCGRG 1 cut(s) 702
EcoO109I RGGNCCY 1 cut(s) 176
EcoRII CCWGG 1 cut(s) 539
EcoT14I CCWWGG 2 cut(s) 49, 682
ErhI CCWWGG 2 cut(s) 49, 682
FaeI CATG 3 cut(s) 410, 419, 495
FaiI YATR 6 cut(s) 342, 408, 417, 493, 677, 774
FaqI GGGAC 1 cut(s) 16
FatI CATG 3 cut(s) 406, 415, 491
FauI CCCGC 1 cut(s) 375
Fnu4HI GCNGC 6 cut(s) 102, 118, 136, 157, 393, 561
FokI GGATG 3 cut(s) 32, 108, 667
Fsp4HI GCNGC 6 cut(s) 102, 118, 136, 157, 393, 561
FspBI CTAG 1 cut(s) 50
GlaI GCGC 1 cut(s) 396
GluI GCNGC 6 cut(s) 102, 118, 136, 157, 393, 561
GsaI CCCAGC 1 cut(s) 504
GsuI CTGGAG 1 cut(s) 515
HaeII RGCGCY 1 cut(s) 398
HaeIII GGCC 4 cut(s) 108, 426, 588, 681
HapII CCGG 6 cut(s) 141, 291, 360, 564, 743, 806
HgaI GACGC 1 cut(s) 751
HhaI GCGC 1 cut(s) 397
Hin1II CATG 3 cut(s) 410, 419, 495
Hin6I GCGC 1 cut(s) 395
HinP1I GCGC 1 cut(s) 395
HincII GTYRAC 1 cut(s) 766
HindII GTYRAC 1 cut(s) 766
HpaII CCGG 6 cut(s) 141, 291, 360, 564, 743, 806
HphI GGTGA 5 cut(s) 34, 356, 529, 728, 821
Hpy166II GTNNAC 3 cut(s) 524, 667, 766
Hpy188III TCNNGA 3 cut(s) 86, 323, 743
Hpy8I GTNNAC 3 cut(s) 524, 667, 766
Hpy99I CGWCG 1 cut(s) 473
HpyAV CCTTC 1 cut(s) 167
HpyCH4III ACNGT 5 cut(s) 218, 287, 315, 607, 716
HpyCH4IV ACGT 1 cut(s) 720
HpyCH4V TGCA 6 cut(s) 77, 117, 392, 495, 667, 786
HpyF3I CTNAG 1 cut(s) 55
HpySE526I ACGT 1 cut(s) 720
Hsp92II CATG 3 cut(s) 410, 419, 495
HspAI GCGC 1 cut(s) 395
Kpn2I TCCGGA 1 cut(s) 742
KpnI GGTACC 1 cut(s) 357
LmnI GCTCC 4 cut(s) 474, 557, 692, 745
Lsp1109I GCAGC 3 cut(s) 129, 404, 547
LweI GCATC 2 cut(s) 136, 316
MaeI CTAG 1 cut(s) 50
MaeII ACGT 1 cut(s) 720
MaeIII GTNAC 6 cut(s) 13, 128, 238, 409, 716, 809
MboII GAAGA 2 cut(s) 31, 455
MhlI GDGCHC 1 cut(s) 669
MlsI TGGCCA 1 cut(s) 426
MluCI AATT 4 cut(s) 18, 72, 122, 550
MluNI TGGCCA 1 cut(s) 426
MnlI CCTC 1 cut(s) 341
Mox20I TGGCCA 1 cut(s) 426
MroI TCCGGA 1 cut(s) 742
MscI TGGCCA 1 cut(s) 426
MseI TTAA 2 cut(s) 255, 267
Msp20I TGGCCA 1 cut(s) 426
MspA1I CMGCKG 1 cut(s) 368
MspI CCGG 6 cut(s) 141, 291, 360, 564, 743, 806
MspR9I CCNGG 2 cut(s) 541, 807
MvaI CCWGG 1 cut(s) 541
MvnI CGCG 1 cut(s) 762
NciI CCSGG 1 cut(s) 807
NlaIII CATG 3 cut(s) 410, 419, 495
NlaIV GGNNCC 4 cut(s) 139, 355, 364, 694
NmeAIII GCCGAG 1 cut(s) 312
NmuCI GTSAC 4 cut(s) 238, 409, 716, 809
PaeR7I CTCGAG 1 cut(s) 702
PceI AGGCCT 1 cut(s) 108
PflFI GACNNNGTC 1 cut(s) 28
PinAI ACCGGT 1 cut(s) 359
PkrI GCNGC 6 cut(s) 103, 119, 137, 158, 394, 562
PpuMI RGGWCCY 1 cut(s) 176
PshBI ATTAAT 1 cut(s) 267
PsiI TTATAA 1 cut(s) 774
Psp5II RGGWCCY 1 cut(s) 176
Psp6I CCWGG 1 cut(s) 539
PspFI CCCAGC 1 cut(s) 500
PspGI CCWGG 1 cut(s) 539
PspN4I GGNNCC 4 cut(s) 139, 355, 364, 694
PspPI GGNCC 2 cut(s) 176, 524
PspPPI RGGWCCY 1 cut(s) 176
PspXI VCTCGAGB 1 cut(s) 702
PsrI GAACNNNNNNTAC 4 cut(s) 346, 378, 536, 568
PsyI GACNNNGTC 1 cut(s) 28
RsaI GTAC 3 cut(s) 184, 355, 512
RsaNI GTAC 3 cut(s) 183, 354, 511
SaqAI TTAA 2 cut(s) 255, 267
SatI GCNGC 6 cut(s) 102, 118, 136, 157, 393, 561
Sau96I GGNCC 2 cut(s) 176, 524
ScaI AGTACT 1 cut(s) 512
ScrFI CCNGG 2 cut(s) 541, 807
SduI GDGCHC 1 cut(s) 669
SfaNI GCATC 2 cut(s) 136, 316
SfcI CTRYAG 1 cut(s) 612
Sfr274I CTCGAG 1 cut(s) 702
SgrAI CRCCGGYG 1 cut(s) 140
SinI GGWCC 2 cut(s) 176, 524
SlaI CTCGAG 1 cut(s) 702
SmlI CTYRAG 2 cut(s) 84, 702
SmoI CTYRAG 2 cut(s) 84, 702
Sse9I AATT 4 cut(s) 18, 72, 122, 550
SseBI AGGCCT 1 cut(s) 108
SsiI CCGC 6 cut(s) 93, 102, 135, 156, 366, 382
SspMI CTAG 1 cut(s) 50
StuI AGGCCT 1 cut(s) 108
StyD4I CCNGG 2 cut(s) 539, 805
StyI CCWWGG 2 cut(s) 49, 682
TaaI ACNGT 5 cut(s) 218, 287, 315, 607, 716
TaiI ACGT 1 cut(s) 723
TaqI TCGA 2 cut(s) 324, 703
TasI AATT 4 cut(s) 18, 72, 122, 550
TatI WGTACW 2 cut(s) 182, 510
TauI GCSGC 3 cut(s) 104, 138, 159
Tru1I TTAA 2 cut(s) 255, 267
Tru9I TTAA 2 cut(s) 255, 267
TscAI CASTG 1 cut(s) 250
TseFI GTSAC 4 cut(s) 238, 409, 716, 809
TseI GCWGC 3 cut(s) 117, 392, 560
Tsp45I GTSAC 4 cut(s) 238, 409, 716, 809
TspGWI ACGGA 2 cut(s) 570, 661
TspRI CASTG 1 cut(s) 250
Tth111I GACNNNGTC 1 cut(s) 28
VneI GTGCAC 1 cut(s) 665
VpaK11BI GGWCC 2 cut(s) 176, 524
VspI ATTAAT 1 cut(s) 267
XapI RAATTY 1 cut(s) 122
XhoI CTCGAG 1 cut(s) 702
XmaJI CCTAGG 1 cut(s) 49
XspI CTAG 1 cut(s) 50
ZrmI AGTACT 1 cut(s) 512
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.