RLG00000010203

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0004556
Physical Location & Seq
Forward (+)
64132721 .. 64134144
1424 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010203

Sequence Viewer

Length: 939 bp
ATGGCTTTCACCAGTGGATATCATATATTCTTATTGTTACTTGCAATTTTGTTCTTCCAATTCATGTACATGTGCACTGCAGATGTCCCATTTCCCAAGCAGAAACGAGTTATAGTCAGAAATGATTTGGGCGGAGCCTTGAATCTTACAATGACATGTACTTCCGTCCGAGAAGCGCCTCGGCCCCTTGCGCTAACTCTAACCCCTCCTCCTCTTGCCGGCGAGGCGCTTAGCGGCTCCGGCCGATTTCCAATCTCAGGGCACCTCTGCCCTTCCATTGATCTATCTTGGCCGGATCTGACCCCTATTATTGAAGGCTATTTTGGCGGAGGTCCTGGAGCCCTGTTAATTTGGGCTGGCAAAAGCCATAGAGGTGGTGAAGGGCTGGCAAATATCTGCTTCATCCACGATAATGACCCGATTTCCTCGTCCTCGTTGCCTCCTACAATGGCTGGAATCGATGTGACGCCTATGTGGCTGCAATTGAACTTCCCCAAGGTTGCTGATGATGCGCTTGTTGAAGCATATAGAGGAGCACTGCTAGTAGGGGGAGGAGCCAACAGAGCCGTTCGGCTGCTGTTGAGTGAGGAGATGAGTTTTGCCGATGCCATTGGGTATGGCAGCAATGTTGATCTTGGGTCTTTAGGGTTTGATCTGTTTGGACCGGAGACCAATGCAAAGGACAGGGGAGCACTACTACAAGCGTGTGACGGTGATGTCACGGTGAGGATGCTGATGTCGGCGAAGCTGGCTGCTGGTGGCGGAATTACATGGGCTCGGGCTCGGGTCCATAACTGGATATGGGTGTCCAGATTAGTATGGCAGGCGGTGTGTCGCTTTTGGGCTCCTATCCGTGCCCAACTCTCGATGGCTTGGACGGTGAAGACTGAGGACTTATCGTCGACTATGTTAGTGTTCAAATCATGGCTGGTTGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

313

Amino Acids

33.75

Weight (kDa)

6.21

Isoelectric Point (pI)

36.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 716
AccB1I GGYRCC 1 cut(s) 261
AccI GTMKAC 1 cut(s) 902
AciI CCGC 5 cut(s) 132, 234, 327, 762, 827
AclWI GGATC 1 cut(s) 303
AcoI YGGCCR 2 cut(s) 241, 290
AcyI GRCGYC 1 cut(s) 467
AfaI GTAC 2 cut(s) 68, 160
AfiI CCNNNNNNNGG 2 cut(s) 218, 257
AflIII ACRYGT 2 cut(s) 69, 155
AgsI TTSAA 6 cut(s) 142, 314, 487, 521, 919, 935
AhdI GACNNNNNGTC 1 cut(s) 898
AjnI CCWGG 1 cut(s) 334
AjuI GAANNNNNNNTTGG 2 cut(s) 306, 338
AluBI AGCT 1 cut(s) 748
AluI AGCT 1 cut(s) 748
Alw21I GWGCWC 3 cut(s) 77, 538, 694
Alw26I GTCTC 1 cut(s) 662
Alw44I GTGCAC 1 cut(s) 73
AlwI GGATC 1 cut(s) 303
Ama87I CYCGRG 2 cut(s) 777, 783
AoxI GGCC 3 cut(s) 182, 241, 290
ApaLI GTGCAC 1 cut(s) 73
ApeKI GCWGC 4 cut(s) 478, 574, 621, 752
AspLEI GCGC 4 cut(s) 178, 193, 229, 514
AspS9I GGNCC 4 cut(s) 183, 332, 662, 787
AsuHPI GGTGA 4 cut(s) 389, 725, 736, 892
AvaI CYCGRG 2 cut(s) 777, 783
AvaII GGWCC 3 cut(s) 332, 662, 787
BaeGI GKGCMC 3 cut(s) 77, 264, 859
BanI GGYRCC 1 cut(s) 261
BanII GRGCYC 4 cut(s) 343, 778, 784, 847
BbsI GAAGAC 1 cut(s) 890
Bbv12I GWGCWC 3 cut(s) 77, 538, 694
BbvI GCAGC 4 cut(s) 465, 561, 633, 739
BccI CCATC 1 cut(s) 862
BceAI ACGGC 1 cut(s) 551
BciT130I CCWGG 1 cut(s) 336
BcoDI GTCTC 1 cut(s) 662
BfaI CTAG 1 cut(s) 542
BfmI CTRYAG 1 cut(s) 78
BfoI RGCGCY 2 cut(s) 179, 230
BglI GCCNNNNNGGC 2 cut(s) 224, 475
BisI GCNGC 5 cut(s) 235, 479, 575, 622, 753
BlpI GCTNAGC 1 cut(s) 230
BlsI GCNGC 5 cut(s) 236, 480, 576, 623, 754
Bme1390I CCNGG 1 cut(s) 336
Bme18I GGWCC 3 cut(s) 332, 662, 787
BmeRI GACNNNNNGTC 1 cut(s) 898
BmeT110I CYCGRG 2 cut(s) 777, 783
BmgT120I GGNCC 4 cut(s) 183, 332, 662, 787
BmiI GGNNCC 8 cut(s) 136, 185, 238, 263, 340, 556, 788, 846
BmrFI CCNGG 1 cut(s) 336
BmsI GCATC 3 cut(s) 499, 595, 720
BpiI GAAGAC 1 cut(s) 890
BpmI CTGGAG 1 cut(s) 357
Bpu1102I GCTNAGC 1 cut(s) 230
Bsa29I ATCGAT 1 cut(s) 459
BsaHI GRCGYC 1 cut(s) 467
BsaI GGTCTC 1 cut(s) 662
BsaJI CCNNGG 2 cut(s) 179, 495
BsaWI WCCGGW 1 cut(s) 664
BsaXI ACNNNNNCTCC 2 cut(s) 193, 223
Bsc4I CCNNNNNNNGG 2 cut(s) 218, 257
Bse118I RCCGGY 1 cut(s) 218
Bse1I ACTGG 2 cut(s) 12, 800
Bse3DI GCAATG 1 cut(s) 631
BseBI CCWGG 1 cut(s) 336
BseCI ATCGAT 1 cut(s) 459
BseDI CCNNGG 2 cut(s) 179, 495
BseGI GGATG 2 cut(s) 402, 735
BseLI CCNNNNNNNGG 2 cut(s) 218, 257
BseMI GCAATG 1 cut(s) 631
BseMII CTCAG 2 cut(s) 270, 879
BseNI ACTGG 2 cut(s) 12, 800
BseRI GAGGAG 5 cut(s) 198, 201, 546, 567, 602
BseSI GKGCMC 3 cut(s) 77, 264, 859
BseX3I CGGCCG 1 cut(s) 241
BseXI GCAGC 4 cut(s) 465, 561, 633, 739
Bsh1285I CGRYCG 1 cut(s) 244
BshFI GGCC 3 cut(s) 184, 243, 292
BshNI GGYRCC 1 cut(s) 261
BshVI ATCGAT 1 cut(s) 459
BsiEI CGRYCG 1 cut(s) 244
BsiHKAI GWGCWC 3 cut(s) 77, 538, 694
BsiHKCI CYCGRG 2 cut(s) 777, 783
BsiSI CCGG 4 cut(s) 219, 240, 293, 665
BslFI GGGAC 1 cut(s) 71
BslI CCNNNNNNNGG 2 cut(s) 218, 257
BsmAI GTCTC 1 cut(s) 662
BsmFI GGGAC 1 cut(s) 71
BsnI GGCC 3 cut(s) 184, 243, 292
Bso31I GGTCTC 1 cut(s) 662
BsoBI CYCGRG 2 cut(s) 777, 783
Bsp1286I GDGCHC 9 cut(s) 77, 264, 343, 538, 694, 778, 784, 847, 859
Bsp1407I TGTACA 1 cut(s) 66
Bsp143I GATC 4 cut(s) 280, 295, 631, 652
Bsp1720I GCTNAGC 1 cut(s) 230
BspACI CCGC 5 cut(s) 132, 234, 327, 762, 827
BspANI GGCC 3 cut(s) 184, 243, 292
BspCNI CTCAG 2 cut(s) 269, 880
BspDI ATCGAT 1 cut(s) 459
BspLI GGNNCC 8 cut(s) 136, 185, 238, 263, 340, 556, 788, 846
BspMAI CTGCAG 1 cut(s) 82
BspPI GGATC 1 cut(s) 303
BspT107I GGYRCC 1 cut(s) 261
BspTNI GGTCTC 1 cut(s) 662
BsrDI GCAATG 1 cut(s) 631
BsrFI RCCGGY 1 cut(s) 218
BsrGI TGTACA 1 cut(s) 66
BsrI ACTGG 2 cut(s) 12, 800
BssAI RCCGGY 1 cut(s) 218
BssECI CCNNGG 2 cut(s) 179, 495
BssMI GATC 4 cut(s) 280, 295, 631, 652
BssNI GRCGYC 1 cut(s) 467
BssT1I CCWWGG 1 cut(s) 495
Bst2UI CCWGG 1 cut(s) 336
Bst4CI ACNGT 3 cut(s) 713, 724, 880
BstACI GRCGYC 1 cut(s) 467
BstAUI TGTACA 1 cut(s) 66
BstC8I GCNNGC 5 cut(s) 220, 358, 387, 750, 825
BstDEI CTNAG 3 cut(s) 230, 256, 888
BstF5I GGATG 2 cut(s) 402, 735
BstH2I RGCGCY 2 cut(s) 179, 230
BstHHI GCGC 4 cut(s) 178, 193, 229, 514
BstKTI GATC 4 cut(s) 283, 298, 634, 655
BstMAI GTCTC 1 cut(s) 662
BstMBI GATC 4 cut(s) 280, 295, 631, 652
BstMCI CGRYCG 1 cut(s) 244
BstMWI GCNNNNNNNGC 8 cut(s) 190, 224, 240, 324, 475, 509, 563, 749
BstNI CCWGG 1 cut(s) 336
BstNSI RCATGY 2 cut(s) 73, 159
BstSCI CCNGG 1 cut(s) 334
BstSFI CTRYAG 1 cut(s) 78
BstSLI GKGCMC 3 cut(s) 77, 264, 859
BstV1I GCAGC 4 cut(s) 465, 561, 633, 739
BstV2I GAAGAC 1 cut(s) 890
BstX2I RGATCY 1 cut(s) 295
BstXI CCANNNNNNTGG 1 cut(s) 374
BstYI RGATCY 1 cut(s) 295
BstZI CGGCCG 1 cut(s) 241
Bsu15I ATCGAT 1 cut(s) 459
BsuRI GGCC 3 cut(s) 184, 243, 292
BsuTUI ATCGAT 1 cut(s) 459
BtsCI GGATG 2 cut(s) 402, 735
BtsI GCAGTG 2 cut(s) 75, 536
BtsIMutI CAGTG 3 cut(s) 19, 75, 536
Cac8I GCNNGC 5 cut(s) 220, 358, 387, 750, 825
CfoI GCGC 4 cut(s) 178, 193, 229, 514
Cfr10I RCCGGY 1 cut(s) 218
Cfr13I GGNCC 4 cut(s) 183, 332, 662, 787
ClaI ATCGAT 1 cut(s) 459
CseI GACGC 1 cut(s) 475
Csp6I GTAC 2 cut(s) 67, 159
CviAII CATG 5 cut(s) 64, 70, 156, 771, 924
CviQI GTAC 2 cut(s) 67, 159
DdeI CTNAG 3 cut(s) 230, 256, 888
DpnI GATC 4 cut(s) 282, 297, 633, 654
DpnII GATC 4 cut(s) 280, 295, 631, 652
DrdI GACNNNNNNGTC 1 cut(s) 716
DriI GACNNNNNGTC 1 cut(s) 898
DseDI GACNNNNNNGTC 1 cut(s) 716
EaeI YGGCCR 2 cut(s) 241, 290
EagI CGGCCG 1 cut(s) 241
Eam1105I GACNNNNNGTC 1 cut(s) 898
EciI GGCGGA 3 cut(s) 147, 342, 777
EclXI CGGCCG 1 cut(s) 241
Eco130I CCWWGG 1 cut(s) 495
Eco24I GRGCYC 4 cut(s) 343, 778, 784, 847
Eco31I GGTCTC 1 cut(s) 662
Eco32I GATATC 1 cut(s) 20
Eco47I GGWCC 3 cut(s) 332, 662, 787
Eco52I CGGCCG 1 cut(s) 241
Eco88I CYCGRG 2 cut(s) 777, 783
EcoO109I RGGNCCY 1 cut(s) 332
EcoRII CCWGG 1 cut(s) 334
EcoRV GATATC 1 cut(s) 20
EcoT14I CCWWGG 1 cut(s) 495
EcoT38I GRGCYC 4 cut(s) 343, 778, 784, 847
ErhI CCWWGG 1 cut(s) 495
FaeI CATG 5 cut(s) 67, 73, 159, 774, 927
FaqI GGGAC 1 cut(s) 71
FatI CATG 5 cut(s) 63, 69, 155, 770, 923
FblI GTMKAC 1 cut(s) 902
Fnu4HI GCNGC 5 cut(s) 235, 479, 575, 622, 753
FokI GGATG 2 cut(s) 389, 742
FriOI GRGCYC 4 cut(s) 343, 778, 784, 847
Fsp4HI GCNGC 5 cut(s) 235, 479, 575, 622, 753
FspBI CTAG 1 cut(s) 542
GlaI GCGC 4 cut(s) 177, 192, 228, 513
GluI GCNGC 5 cut(s) 235, 479, 575, 622, 753
GsuI CTGGAG 1 cut(s) 357
HaeII RGCGCY 2 cut(s) 179, 230
HaeIII GGCC 3 cut(s) 184, 243, 292
HapII CCGG 4 cut(s) 219, 240, 293, 665
HgaI GACGC 1 cut(s) 475
HhaI GCGC 4 cut(s) 178, 193, 229, 514
Hin1I GRCGYC 1 cut(s) 467
Hin1II CATG 5 cut(s) 67, 73, 159, 774, 927
Hin6I GCGC 4 cut(s) 176, 191, 227, 512
HinP1I GCGC 4 cut(s) 176, 191, 227, 512
HincII GTYRAC 1 cut(s) 903
HindII GTYRAC 1 cut(s) 903
HinfI GANTC 2 cut(s) 142, 456
HpaII CCGG 4 cut(s) 219, 240, 293, 665
HphI GGTGA 4 cut(s) 389, 725, 736, 892
Hpy166II GTNNAC 2 cut(s) 75, 903
Hpy188I TCNGA 3 cut(s) 119, 170, 300
Hpy188III TCNNGA 2 cut(s) 810, 865
Hpy8I GTNNAC 2 cut(s) 75, 903
Hpy99I CGWCG 1 cut(s) 904
HpyAV CCTTC 3 cut(s) 282, 308, 374
HpyCH4III ACNGT 3 cut(s) 713, 724, 880
HpyCH4V TGCA 5 cut(s) 44, 75, 80, 481, 677
HpyF10VI GCNNNNNNNGC 8 cut(s) 190, 224, 240, 324, 475, 509, 563, 749
HpyF3I CTNAG 3 cut(s) 230, 256, 888
Hsp92I GRCGYC 1 cut(s) 467
Hsp92II CATG 5 cut(s) 67, 73, 159, 774, 927
HspAI GCGC 4 cut(s) 176, 191, 227, 512
KroI GCCGGC 1 cut(s) 218
KroNI GCCGGC 1 cut(s) 220
Kzo9I GATC 4 cut(s) 280, 295, 631, 652
LmnI GCTCC 7 cut(s) 134, 242, 338, 533, 554, 689, 850
Lsp1109I GCAGC 4 cut(s) 465, 561, 633, 739
LweI GCATC 3 cut(s) 499, 595, 720
MaeI CTAG 1 cut(s) 542
MaeIII GTNAC 4 cut(s) 36, 463, 707, 718
MalI GATC 4 cut(s) 282, 297, 633, 654
MboI GATC 4 cut(s) 280, 295, 631, 652
MboII GAAGA 2 cut(s) 46, 895
MfeI CAATTG 1 cut(s) 482
MflI RGATCY 1 cut(s) 295
MhlI GDGCHC 9 cut(s) 77, 264, 343, 538, 694, 778, 784, 847, 859
MluCI AATT 5 cut(s) 45, 59, 348, 482, 765
MroNI GCCGGC 1 cut(s) 218
MseI TTAA 1 cut(s) 347
MslI CAYNNNNRTG 3 cut(s) 68, 372, 411
MspI CCGG 4 cut(s) 219, 240, 293, 665
MspR9I CCNGG 1 cut(s) 336
MunI CAATTG 1 cut(s) 482
MvaI CCWGG 1 cut(s) 336
MwoI GCNNNNNNNGC 8 cut(s) 190, 224, 240, 324, 475, 509, 563, 749
NaeI GCCGGC 1 cut(s) 220
NdeII GATC 4 cut(s) 280, 295, 631, 652
NgoMIV GCCGGC 1 cut(s) 218
NlaIII CATG 5 cut(s) 67, 73, 159, 774, 927
NlaIV GGNNCC 8 cut(s) 136, 185, 238, 263, 340, 556, 788, 846
NmeAIII GCCGAG 1 cut(s) 160
NmuCI GTSAC 3 cut(s) 463, 707, 718
NspI RCATGY 2 cut(s) 73, 159
PciI ACATGT 2 cut(s) 69, 155
PdiI GCCGGC 1 cut(s) 220
PfeI GAWTC 2 cut(s) 142, 456
PfoI TCCNGGA 1 cut(s) 334
PkrI GCNGC 5 cut(s) 236, 480, 576, 623, 754
PpuMI RGGWCCY 1 cut(s) 332
PscI ACATGT 2 cut(s) 69, 155
Psp5II RGGWCCY 1 cut(s) 332
Psp6I CCWGG 1 cut(s) 334
PspGI CCWGG 1 cut(s) 334
PspN4I GGNNCC 8 cut(s) 136, 185, 238, 263, 340, 556, 788, 846
PspPI GGNCC 4 cut(s) 183, 332, 662, 787
PspPPI RGGWCCY 1 cut(s) 332
PstI CTGCAG 1 cut(s) 82
PsuI RGATCY 1 cut(s) 295
RsaI GTAC 2 cut(s) 68, 160
RsaNI GTAC 2 cut(s) 67, 159
RseI CAYNNNNRTG 3 cut(s) 68, 372, 411
SalI GTCGAC 1 cut(s) 901
SaqAI TTAA 1 cut(s) 347
SatI GCNGC 5 cut(s) 235, 479, 575, 622, 753
Sau3AI GATC 4 cut(s) 280, 295, 631, 652
Sau96I GGNCC 4 cut(s) 183, 332, 662, 787
ScrFI CCNGG 1 cut(s) 336
SduI GDGCHC 9 cut(s) 77, 264, 343, 538, 694, 778, 784, 847, 859
SetI ASST 5 cut(s) 267, 334, 376, 501, 750
SfaNI GCATC 3 cut(s) 499, 595, 720
SfcI CTRYAG 1 cut(s) 78
SinI GGWCC 3 cut(s) 332, 662, 787
SmiMI CAYNNNNRTG 3 cut(s) 68, 372, 411
Sse9I AATT 5 cut(s) 45, 59, 348, 482, 765
SsiI CCGC 5 cut(s) 132, 234, 327, 762, 827
SspMI CTAG 1 cut(s) 542
StyD4I CCNGG 1 cut(s) 334
StyI CCWWGG 1 cut(s) 495
TaaI ACNGT 3 cut(s) 713, 724, 880
TaqI TCGA 3 cut(s) 459, 866, 902
TasI AATT 5 cut(s) 45, 59, 348, 482, 765
TatI WGTACW 2 cut(s) 66, 158
TauI GCSGC 1 cut(s) 237
TfiI GAWTC 2 cut(s) 142, 456
Tru1I TTAA 1 cut(s) 347
Tru9I TTAA 1 cut(s) 347
TscAI CASTG 3 cut(s) 19, 82, 543
TseFI GTSAC 3 cut(s) 463, 707, 718
TseI GCWGC 4 cut(s) 478, 574, 621, 752
Tsp45I GTSAC 3 cut(s) 463, 707, 718
TspDTI ATGAA 2 cut(s) 52, 391
TspGWI ACGGA 2 cut(s) 154, 842
TspRI CASTG 3 cut(s) 19, 82, 543
VneI GTGCAC 1 cut(s) 73
VpaK11BI GGWCC 3 cut(s) 332, 662, 787
XceI RCATGY 2 cut(s) 73, 159
XmiI GTMKAC 1 cut(s) 902
XspI CTAG 1 cut(s) 542
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.