Rh5BG177200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0004556
Physical Location & Seq
Reverse (-)
19139268 .. 19139546
279 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG177200.1

Sequence Viewer

Length: 279 bp
ATGAAAACCCTATTCTCTCTAGCAGCGCCTCCCCGTGCGCTCACTCTAACGCAGCCTCCTCTTGCCGGCCGGTTTCCAATATCAGGGCACCTCTGCCCTTCCATTGATCTATCTTTGCCAGATCTGACCCATACTTATGGAGGCAGTCTTGTAGCCCTGTTAATTTGGGTTGGCAAAAGCCATGGCGGTGGTGAAAGGGTGGAACGCTTCATCCACGATGGTGACCCGGTTTCCTCGTCCTGGCTGCCTCCTACAATTGAACATCCCCGAGGCTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

92

Amino Acids

9.85

Weight (kDa)

6.95

Isoelectric Point (pI)

36.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 87
AciI CCGC 1 cut(s) 186
AcoI YGGCCR 1 cut(s) 67
AfiI CCNNNNNNNGG 3 cut(s) 65, 83, 240
AgsI TTSAA 1 cut(s) 260
AjnI CCWGG 1 cut(s) 239
AleI CACNNNNGTG 1 cut(s) 219
AloI GAACNNNNNNTCC 2 cut(s) 195, 227
Ama87I CYCGRG 1 cut(s) 267
AoxI GGCC 1 cut(s) 67
ApeKI GCWGC 4 cut(s) 23, 52, 244, 273
AspLEI GCGC 2 cut(s) 28, 40
AsuC2I CCSGG 1 cut(s) 227
AsuHPI GGTGA 2 cut(s) 203, 233
AvaI CYCGRG 1 cut(s) 267
BaeGI GKGCMC 1 cut(s) 90
BanI GGYRCC 1 cut(s) 87
BbvI GCAGC 4 cut(s) 35, 64, 231, 260
BccI CCATC 1 cut(s) 212
BciT130I CCWGG 1 cut(s) 241
BcnI CCSGG 1 cut(s) 227
BfaI CTAG 1 cut(s) 20
BfoI RGCGCY 1 cut(s) 29
BglII AGATCT 1 cut(s) 121
BisI GCNGC 4 cut(s) 24, 53, 245, 274
BlsI GCNGC 4 cut(s) 25, 54, 246, 275
Bme1390I CCNGG 2 cut(s) 227, 241
BmeT110I CYCGRG 1 cut(s) 267
BmiI GGNNCC 1 cut(s) 89
BmrFI CCNGG 2 cut(s) 227, 241
BpuMI CCSGG 1 cut(s) 227
BsaJI CCNNGG 2 cut(s) 181, 268
BsaXI ACNNNNNCTCC 2 cut(s) 40, 70
Bsc4I CCNNNNNNNGG 3 cut(s) 65, 83, 240
Bse118I RCCGGY 2 cut(s) 65, 69
BseBI CCWGG 1 cut(s) 241
BseDI CCNNGG 2 cut(s) 181, 268
BseGI GGATG 2 cut(s) 210, 262
BseLI CCNNNNNNNGG 3 cut(s) 65, 83, 240
BseRI GAGGAG 1 cut(s) 48
BseSI GKGCMC 1 cut(s) 90
BseX3I CGGCCG 1 cut(s) 67
BseXI GCAGC 4 cut(s) 35, 64, 231, 260
Bsh1285I CGRYCG 1 cut(s) 70
BshFI GGCC 1 cut(s) 69
BshNI GGYRCC 1 cut(s) 87
BsiEI CGRYCG 1 cut(s) 70
BsiHKCI CYCGRG 1 cut(s) 267
BsiSI CCGG 3 cut(s) 66, 70, 227
BslI CCNNNNNNNGG 3 cut(s) 65, 83, 240
BsnI GGCC 1 cut(s) 69
BsoBI CYCGRG 1 cut(s) 267
Bsp1286I GDGCHC 1 cut(s) 90
Bsp143I GATC 2 cut(s) 106, 121
Bsp19I CCATGG 1 cut(s) 181
BspACI CCGC 1 cut(s) 186
BspANI GGCC 1 cut(s) 69
BspLI GGNNCC 1 cut(s) 89
BspT107I GGYRCC 1 cut(s) 87
BsrFI RCCGGY 2 cut(s) 65, 69
BssAI RCCGGY 2 cut(s) 65, 69
BssECI CCNNGG 2 cut(s) 181, 268
BssMI GATC 2 cut(s) 106, 121
BssT1I CCWWGG 1 cut(s) 181
Bst2UI CCWGG 1 cut(s) 241
BstC8I GCNNGC 1 cut(s) 67
BstDSI CCRYGG 1 cut(s) 181
BstEII GGTNACC 1 cut(s) 221
BstF5I GGATG 2 cut(s) 210, 262
BstH2I RGCGCY 1 cut(s) 29
BstHHI GCGC 2 cut(s) 28, 40
BstKTI GATC 2 cut(s) 109, 124
BstMBI GATC 2 cut(s) 106, 121
BstMCI CGRYCG 1 cut(s) 70
BstNI CCWGG 1 cut(s) 241
BstPI GGTNACC 1 cut(s) 221
BstSCI CCNGG 2 cut(s) 225, 239
BstSLI GKGCMC 1 cut(s) 90
BstV1I GCAGC 4 cut(s) 35, 64, 231, 260
BstX2I RGATCY 1 cut(s) 121
BstXI CCANNNNNNTGG 2 cut(s) 137, 188
BstYI RGATCY 1 cut(s) 121
BstZI CGGCCG 1 cut(s) 67
BsuRI GGCC 1 cut(s) 69
BtgI CCRYGG 1 cut(s) 181
BtsCI GGATG 2 cut(s) 210, 262
Cac8I GCNNGC 1 cut(s) 67
CfoI GCGC 2 cut(s) 28, 40
Cfr10I RCCGGY 2 cut(s) 65, 69
CviAII CATG 1 cut(s) 182
CviJI RGCY 6 cut(s) 55, 69, 155, 180, 244, 273
CviKI_1 RGCY 6 cut(s) 55, 69, 155, 180, 244, 273
DpnI GATC 2 cut(s) 108, 123
DpnII GATC 2 cut(s) 106, 121
EaeI YGGCCR 1 cut(s) 67
EagI CGGCCG 1 cut(s) 67
EclXI CGGCCG 1 cut(s) 67
Eco130I CCWWGG 1 cut(s) 181
Eco52I CGGCCG 1 cut(s) 67
Eco88I CYCGRG 1 cut(s) 267
Eco91I GGTNACC 1 cut(s) 221
EcoO65I GGTNACC 1 cut(s) 221
EcoRII CCWGG 1 cut(s) 239
EcoT14I CCWWGG 1 cut(s) 181
ErhI CCWWGG 1 cut(s) 181
FaeI CATG 1 cut(s) 185
FaiI YATR 3 cut(s) 132, 138, 183
FatI CATG 1 cut(s) 181
Fnu4HI GCNGC 4 cut(s) 24, 53, 245, 274
FokI GGATG 2 cut(s) 197, 249
Fsp4HI GCNGC 4 cut(s) 24, 53, 245, 274
FspBI CTAG 1 cut(s) 20
GlaI GCGC 2 cut(s) 27, 39
GluI GCNGC 4 cut(s) 24, 53, 245, 274
HaeII RGCGCY 1 cut(s) 29
HaeIII GGCC 1 cut(s) 69
HapII CCGG 3 cut(s) 66, 70, 227
HhaI GCGC 2 cut(s) 28, 40
Hin1II CATG 1 cut(s) 185
Hin6I GCGC 2 cut(s) 26, 38
HinP1I GCGC 2 cut(s) 26, 38
HpaII CCGG 3 cut(s) 66, 70, 227
HphI GGTGA 2 cut(s) 203, 233
Hpy188I TCNGA 1 cut(s) 126
HpyAV CCTTC 1 cut(s) 108
Hsp92II CATG 1 cut(s) 185
HspAI GCGC 2 cut(s) 26, 38
KroI GCCGGC 1 cut(s) 65
KroNI GCCGGC 1 cut(s) 67
Kzo9I GATC 2 cut(s) 106, 121
LpnPI CCDG 8 cut(s) 69, 79, 83, 132, 170, 226, 240, 253
Lsp1109I GCAGC 4 cut(s) 35, 64, 231, 260
MaeI CTAG 1 cut(s) 20
MaeIII GTNAC 1 cut(s) 221
MalI GATC 2 cut(s) 108, 123
MboI GATC 2 cut(s) 106, 121
MfeI CAATTG 1 cut(s) 255
MflI RGATCY 1 cut(s) 121
MhlI GDGCHC 1 cut(s) 90
MluCI AATT 2 cut(s) 162, 255
MnlI CCTC 8 cut(s) 39, 66, 69, 101, 134, 244, 258, 263
MroNI GCCGGC 1 cut(s) 65
MseI TTAA 1 cut(s) 161
MslI CAYNNNNRTG 3 cut(s) 135, 186, 219
MspI CCGG 3 cut(s) 66, 70, 227
MspR9I CCNGG 2 cut(s) 227, 241
MunI CAATTG 1 cut(s) 255
MvaI CCWGG 1 cut(s) 241
NaeI GCCGGC 1 cut(s) 67
NciI CCSGG 1 cut(s) 227
NcoI CCATGG 1 cut(s) 181
NdeII GATC 2 cut(s) 106, 121
NgoMIV GCCGGC 1 cut(s) 65
NlaIII CATG 1 cut(s) 185
NlaIV GGNNCC 1 cut(s) 89
NmuCI GTSAC 1 cut(s) 221
OliI CACNNNNGTG 1 cut(s) 219
PdiI GCCGGC 1 cut(s) 67
PkrI GCNGC 4 cut(s) 25, 54, 246, 275
Psp6I CCWGG 1 cut(s) 239
PspEI GGTNACC 1 cut(s) 221
PspGI CCWGG 1 cut(s) 239
PspN4I GGNNCC 1 cut(s) 89
PsuI RGATCY 1 cut(s) 121
RseI CAYNNNNRTG 3 cut(s) 135, 186, 219
SaqAI TTAA 1 cut(s) 161
SatI GCNGC 4 cut(s) 24, 53, 245, 274
Sau3AI GATC 2 cut(s) 106, 121
ScrFI CCNGG 2 cut(s) 227, 241
SduI GDGCHC 1 cut(s) 90
SetI ASST 1 cut(s) 93
SmiMI CAYNNNNRTG 3 cut(s) 135, 186, 219
Sse9I AATT 2 cut(s) 162, 255
SsiI CCGC 1 cut(s) 186
SspMI CTAG 1 cut(s) 20
StyD4I CCNGG 2 cut(s) 225, 239
StyI CCWWGG 1 cut(s) 181
TasI AATT 2 cut(s) 162, 255
Tru1I TTAA 1 cut(s) 161
Tru9I TTAA 1 cut(s) 161
TseFI GTSAC 1 cut(s) 221
TseI GCWGC 4 cut(s) 23, 52, 244, 273
Tsp45I GTSAC 1 cut(s) 221
TspDTI ATGAA 2 cut(s) 17, 199
XspI CTAG 1 cut(s) 20
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.