RLG00000014226

reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0003183
Physical Location & Seq
Reverse (-)
49576953 .. 49578978
2026 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014226

Sequence Viewer

Length: 930 bp
ATGGAGAAGAAGAAGAGCAGAGTACTGATAATCGGAGCAACAGGAAATCTGGGTCACCATTTAGCCAATGCCAGCCTCCATTTCTCTCACCCAACTTTCGCTCTCGTCCGACCCGACACCTTCTCCGACCCCCACAAGTCCCAAAACCTTCGAGCCCTCTCCGATGCCGGCGTCACACTCCTCCAAGGTTCGCTGGAAGATGAAGAGAGCCTTGTTGAAGCCGTGAAGCAAGTTGATGTTGTTGTTTGTGCTGTTCAGGCCAAACAGGTGCTCCATCAGAAGCTTCTTATCCAAGTTATCAAAAATGCTGGCTCCATCAAGAGATTCATTCCATCAGAATTCGGGTTGGACCCAGATAAGACGCAAATTTCAGGGATGGACTACAACTTCTATTCAAACAAAGCCGAGATTAGGCGTCTTGTGGAAGCTCAAGGCATTCCCTACACTTTTGTTTCCTGCAATTTCTATACGAGCTATTTGCTTCCTTCACTTGTGCAGCCGGGCCTCAAAGTGCCTCCGAAGGACAAAGTCACGATTTTCGGGGATGGAAATACAAAAGGCGTTTTTGTCAAGGAAATTGATGTAGCTGCCTTCACTATCAGGACATTGGACGATCCACGAACCTTGAACAAAGTGTTGTATTTTAGGCCACCCGGAAATGTATACTCACTGAATGAACTTGTTGAGCTTTGGGAGAGTAAGATTGGGAAGAAGCTCGAGAAGGTGTTTGTATCAGAAGAAGAGCTTCTTAAGAAGATTAAAGAGACTTCATATCCTGACAACATGGCCCTGATTTTCATATATTCTGCTTTCATAAAGGGCGATCAAACTTACTTTGATATCGAGTCAGCTGATAGTGCCGATGCGACAAAACTGTATCCAGAGCTGAATTATACTACAATCAGTCAATATTTAGACACTCTATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0010283 GO:0016491 GO:0016627 GO:0016628 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NmrA

Protein Analysis

310

Amino Acids

34.63

Weight (kDa)

6.11

Isoelectric Point (pI)

28.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 4 - 305 3.6e-82 NmrA-like family
NAD_binding_10 PF13460 12 - 206 3.2e-16 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 663
AclWI GGATC 1 cut(s) 608
AcsI RAATTY 2 cut(s) 338, 366
AcyI GRCGYC 2 cut(s) 171, 415
AfaI GTAC 1 cut(s) 24
AfiI CCNNNNNNNGG 1 cut(s) 411
AflII CTTAAG 1 cut(s) 749
AgsI TTSAA 3 cut(s) 218, 396, 628
AluBI AGCT 9 cut(s) 283, 428, 474, 587, 688, 715, 745, 851, 886
AluI AGCT 9 cut(s) 283, 428, 474, 587, 688, 715, 745, 851, 886
Alw21I GWGCWC 1 cut(s) 273
Alw26I GTCTC 1 cut(s) 758
AlwI GGATC 1 cut(s) 608
Ama87I CYCGRG 1 cut(s) 716
AoxI GGCC 4 cut(s) 258, 502, 647, 786
ApeKI GCWGC 2 cut(s) 496, 587
ApoI RAATTY 2 cut(s) 338, 366
Asp700I GAANNNNTTC 1 cut(s) 744
AspS9I GGNCC 3 cut(s) 349, 502, 787
AsuC2I CCSGG 2 cut(s) 501, 654
AsuHPI GGTGA 2 cut(s) 47, 80
AvaI CYCGRG 1 cut(s) 716
AvaII GGWCC 1 cut(s) 349
BanII GRGCYC 1 cut(s) 157
Bbv12I GWGCWC 1 cut(s) 273
BbvI GCAGC 2 cut(s) 508, 574
BccI CCATC 5 cut(s) 282, 323, 340, 370, 539
BceAI ACGGC 1 cut(s) 206
BcgI CGANNNNNNTGC 2 cut(s) 460, 494
BciVI GTATCC 1 cut(s) 888
BcnI CCSGG 2 cut(s) 501, 654
BcoDI GTCTC 1 cut(s) 758
BfrI CTTAAG 1 cut(s) 749
BfuI GTATCC 1 cut(s) 888
BisI GCNGC 2 cut(s) 497, 588
BlsI GCNGC 2 cut(s) 498, 589
BmcAI AGTACT 1 cut(s) 24
Bme1390I CCNGG 2 cut(s) 501, 654
Bme18I GGWCC 1 cut(s) 349
BmeT110I CYCGRG 1 cut(s) 716
BmgT120I GGNCC 3 cut(s) 349, 502, 787
BmiI GGNNCC 2 cut(s) 313, 351
BmrFI CCNGG 2 cut(s) 501, 654
BmsI GCATC 2 cut(s) 154, 853
BpuEI CTTGAG 1 cut(s) 414
BpuMI CCSGG 2 cut(s) 501, 654
BsaHI GRCGYC 2 cut(s) 171, 415
BsaJI CCNNGG 1 cut(s) 184
BsaXI ACNNNNNCTCC 4 cut(s) 107, 137, 255, 285
Bsc4I CCNNNNNNNGG 1 cut(s) 411
Bse118I RCCGGY 1 cut(s) 167
BseDI CCNNGG 1 cut(s) 184
BseGI GGATG 2 cut(s) 381, 550
BseLI CCNNNNNNNGG 1 cut(s) 411
BseRI GAGGAG 1 cut(s) 170
BseXI GCAGC 2 cut(s) 508, 574
BsgI GTGCAG 1 cut(s) 515
BshFI GGCC 4 cut(s) 260, 504, 649, 788
BsiHKAI GWGCWC 1 cut(s) 273
BsiHKCI CYCGRG 1 cut(s) 716
BsiSI CCGG 3 cut(s) 168, 500, 654
BslFI GGGAC 1 cut(s) 124
BslI CCNNNNNNNGG 1 cut(s) 411
BsmAI GTCTC 1 cut(s) 758
BsmFI GGGAC 1 cut(s) 124
BsmI GAATGC 1 cut(s) 435
BsnI GGCC 4 cut(s) 260, 504, 649, 788
BsoBI CYCGRG 1 cut(s) 716
Bsp1286I GDGCHC 2 cut(s) 157, 273
Bsp143I GATC 2 cut(s) 613, 823
BspANI GGCC 4 cut(s) 260, 504, 649, 788
BspLI GGNNCC 2 cut(s) 313, 351
BspPI GGATC 1 cut(s) 608
BspQI GCTCTTC 2 cut(s) 8, 735
BspTI CTTAAG 1 cut(s) 749
BsrFI RCCGGY 1 cut(s) 167
BssAI RCCGGY 1 cut(s) 167
BssECI CCNNGG 1 cut(s) 184
BssMI GATC 2 cut(s) 613, 823
BssNAI GTATAC 1 cut(s) 664
BssNI GRCGYC 2 cut(s) 171, 415
BssT1I CCWWGG 1 cut(s) 184
Bst1107I GTATAC 1 cut(s) 664
Bst4CI ACNGT 1 cut(s) 876
Bst6I CTCTTC 3 cut(s) 8, 198, 735
BstACI GRCGYC 2 cut(s) 171, 415
BstAFI CTTAAG 1 cut(s) 749
BstC8I GCNNGC 3 cut(s) 73, 169, 310
BstEII GGTNACC 1 cut(s) 53
BstF5I GGATG 2 cut(s) 381, 550
BstKTI GATC 2 cut(s) 616, 826
BstMAI GTCTC 1 cut(s) 758
BstMBI GATC 2 cut(s) 613, 823
BstMWI GCNNNNNNNGC 2 cut(s) 257, 857
BstPI GGTNACC 1 cut(s) 53
BstSCI CCNGG 2 cut(s) 499, 652
BstV1I GCAGC 2 cut(s) 508, 574
BstZ17I GTATAC 1 cut(s) 664
BsuI GTATCC 1 cut(s) 888
BsuRI GGCC 4 cut(s) 260, 504, 649, 788
BtsCI GGATG 2 cut(s) 381, 550
BtsIMutI CAGTG 1 cut(s) 668
Cac8I GCNNGC 3 cut(s) 73, 169, 310
Cfr10I RCCGGY 1 cut(s) 167
Cfr13I GGNCC 3 cut(s) 349, 502, 787
CseI GACGC 3 cut(s) 160, 370, 404
Csp6I GTAC 1 cut(s) 23
CspCI CAANNNNNGTGG 2 cut(s) 606, 641
CviAII CATG 1 cut(s) 784
CviQI GTAC 1 cut(s) 23
DpnI GATC 2 cut(s) 615, 825
DpnII GATC 2 cut(s) 613, 823
Eam1104I CTCTTC 3 cut(s) 8, 198, 735
EarI CTCTTC 3 cut(s) 8, 198, 735
Eco130I CCWWGG 1 cut(s) 184
Eco24I GRGCYC 1 cut(s) 157
Eco32I GATATC 1 cut(s) 841
Eco47I GGWCC 1 cut(s) 349
Eco88I CYCGRG 1 cut(s) 716
Eco91I GGTNACC 1 cut(s) 53
EcoO65I GGTNACC 1 cut(s) 53
EcoRI GAATTC 1 cut(s) 338
EcoRV GATATC 1 cut(s) 841
EcoT14I CCWWGG 1 cut(s) 184
EcoT38I GRGCYC 1 cut(s) 157
ErhI CCWWGG 1 cut(s) 184
FaeI CATG 1 cut(s) 787
FaiI YATR 8 cut(s) 468, 664, 772, 785, 800, 802, 815, 894
FalI AAGNNNNNCTT 6 cut(s) 195, 227, 729, 761, 732, 764
FaqI GGGAC 1 cut(s) 124
FatI CATG 1 cut(s) 783
FblI GTMKAC 1 cut(s) 663
Fnu4HI GCNGC 2 cut(s) 497, 588
FokI GGATG 2 cut(s) 388, 557
FriOI GRGCYC 1 cut(s) 157
Fsp4HI GCNGC 2 cut(s) 497, 588
GluI GCNGC 2 cut(s) 497, 588
HaeIII GGCC 4 cut(s) 260, 504, 649, 788
HapII CCGG 3 cut(s) 168, 500, 654
HgaI GACGC 3 cut(s) 160, 370, 404
Hin1I GRCGYC 2 cut(s) 171, 415
Hin1II CATG 1 cut(s) 787
HindIII AAGCTT 1 cut(s) 281
HinfI GANTC 2 cut(s) 324, 845
HpaII CCGG 3 cut(s) 168, 500, 654
HphI GGTGA 2 cut(s) 47, 80
Hpy166II GTNNAC 1 cut(s) 664
Hpy188I TCNGA 8 cut(s) 35, 110, 127, 163, 279, 337, 519, 736
Hpy188III TCNNGA 6 cut(s) 319, 532, 601, 718, 776, 881
Hpy8I GTNNAC 1 cut(s) 664
HpyAV CCTTC 6 cut(s) 130, 158, 495, 514, 601, 715
HpyCH4III ACNGT 1 cut(s) 876
HpyCH4V TGCA 2 cut(s) 459, 496
HpyF10VI GCNNNNNNNGC 2 cut(s) 257, 857
Hsp92I GRCGYC 2 cut(s) 171, 415
Hsp92II CATG 1 cut(s) 787
KroI GCCGGC 1 cut(s) 167
KroNI GCCGGC 1 cut(s) 169
Kzo9I GATC 2 cut(s) 613, 823
LguI GCTCTTC 2 cut(s) 8, 735
LmnI GCTCC 3 cut(s) 35, 276, 317
Lsp1109I GCAGC 2 cut(s) 508, 574
LweI GCATC 2 cut(s) 154, 853
MaeIII GTNAC 3 cut(s) 53, 172, 529
MalI GATC 2 cut(s) 615, 825
MboI GATC 2 cut(s) 613, 823
MboII GAAGA 9 cut(s) 19, 22, 25, 209, 215, 721, 749, 752, 766
MhlI GDGCHC 2 cut(s) 157, 273
MluCI AATT 5 cut(s) 338, 366, 460, 576, 889
MlyI GAGTC 1 cut(s) 854
MmeI TCCRAC 3 cut(s) 133, 150, 327
MnlI CCTC 5 cut(s) 86, 167, 191, 515, 525
MroNI GCCGGC 1 cut(s) 167
MroXI GAANNNNTTC 1 cut(s) 744
MseI TTAA 2 cut(s) 750, 759
MspA1I CMGCKG 1 cut(s) 851
MspCI CTTAAG 1 cut(s) 749
MspI CCGG 3 cut(s) 168, 500, 654
MspR9I CCNGG 2 cut(s) 501, 654
Mva1269I GAATGC 1 cut(s) 435
MwoI GCNNNNNNNGC 2 cut(s) 257, 857
NaeI GCCGGC 1 cut(s) 169
NciI CCSGG 2 cut(s) 501, 654
NdeII GATC 2 cut(s) 613, 823
NgoMIV GCCGGC 1 cut(s) 167
NlaIII CATG 1 cut(s) 787
NlaIV GGNNCC 2 cut(s) 313, 351
NmeAIII GCCGAG 1 cut(s) 430
NmuCI GTSAC 3 cut(s) 53, 172, 529
PaeR7I CTCGAG 1 cut(s) 716
PciSI GCTCTTC 2 cut(s) 8, 735
PcsI WCGNNNNNNNCGW 1 cut(s) 111
PctI GAATGC 1 cut(s) 435
PdiI GCCGGC 1 cut(s) 169
PdmI GAANNNNTTC 1 cut(s) 744
PfeI GAWTC 1 cut(s) 324
PflFI GACNNNGTC 1 cut(s) 527
PkrI GCNGC 2 cut(s) 498, 589
PleI GAGTC 1 cut(s) 853
PpsI GAGTC 1 cut(s) 853
PspEI GGTNACC 1 cut(s) 53
PspN4I GGNNCC 2 cut(s) 313, 351
PspPI GGNCC 3 cut(s) 349, 502, 787
PsyI GACNNNGTC 1 cut(s) 527
PvuII CAGCTG 1 cut(s) 851
RsaI GTAC 1 cut(s) 24
RsaNI GTAC 1 cut(s) 23
SapI GCTCTTC 2 cut(s) 8, 735
SaqAI TTAA 2 cut(s) 750, 759
SatI GCNGC 2 cut(s) 497, 588
Sau3AI GATC 2 cut(s) 613, 823
Sau96I GGNCC 3 cut(s) 349, 502, 787
ScaI AGTACT 1 cut(s) 24
SchI GAGTC 1 cut(s) 854
ScrFI CCNGG 2 cut(s) 501, 654
SduI GDGCHC 2 cut(s) 157, 273
SfaNI GCATC 2 cut(s) 154, 853
Sfr274I CTCGAG 1 cut(s) 716
SinI GGWCC 1 cut(s) 349
SlaI CTCGAG 1 cut(s) 716
SmlI CTYRAG 3 cut(s) 429, 716, 749
SmoI CTYRAG 3 cut(s) 429, 716, 749
Sse9I AATT 5 cut(s) 338, 366, 460, 576, 889
SspI AATATT 1 cut(s) 911
StyD4I CCNGG 2 cut(s) 499, 652
StyI CCWWGG 1 cut(s) 184
TaaI ACNGT 1 cut(s) 876
TaqI TCGA 3 cut(s) 151, 717, 843
TasI AATT 5 cut(s) 338, 366, 460, 576, 889
TatI WGTACW 1 cut(s) 22
TfiI GAWTC 1 cut(s) 324
Tru1I TTAA 2 cut(s) 750, 759
Tru9I TTAA 2 cut(s) 750, 759
TscAI CASTG 1 cut(s) 675
TseFI GTSAC 3 cut(s) 53, 172, 529
TseI GCWGC 2 cut(s) 496, 587
Tsp45I GTSAC 3 cut(s) 53, 172, 529
TspDTI ATGAA 6 cut(s) 216, 316, 690, 759, 787, 802
TspRI CASTG 1 cut(s) 675
Tth111I GACNNNGTC 1 cut(s) 527
Vha464I CTTAAG 1 cut(s) 749
VpaK11BI GGWCC 1 cut(s) 349
XapI RAATTY 2 cut(s) 338, 366
XhoI CTCGAG 1 cut(s) 716
XmiI GTMKAC 1 cut(s) 663
XmnI GAANNNNTTC 1 cut(s) 744
ZrmI AGTACT 1 cut(s) 24
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.