Rh4AG031500

isoflavone

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0003183
Physical Location & Seq
Reverse (-)
6800544 .. 6812899
12356 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG031500.1

Sequence Viewer

Length: 993 bp
ATGCTCGTGGTGTTTATGAACACCGATTCTTCACCGATTAAGGGTTCGCTGGAAGACGAAGAGAACCTTGTTGGAGCAGTGAAGCAAGTTGATGTTGTGATTTGTGCTGTTCAGGTCAAACAGAGATTCATTCCATCAGAATTCGGGTTGGACCCAGATAAGACGCAAATTTCAGGGATGGACTACAACTTCTATTCAAACAAAGCCGAGATTAGGCGTCTTGTGGAAGCTCAAGGCATTCCCTACACTTTTATTTCCTGCAATTTCTATACGAGCTATTTGCTTCCTTCACTTGTGCAGCCGGGCCTCAAAGTGCCTCCAAGGGACAAAGTCACGATTTTCGGGGACGGAAATACTAAAGGCGTTTTTGTCAAGGAAATTGATGTAGCTGCCTTCACTATCAGGACATTGGACGATCCACGAACCTTGAACAAAGTGTTGTATTTTAGGCCACCCGGAAATGTGTACTCGCTAAATGAACTTGTTGAGCTTTGGGAGAGTAAGATTGGGAAGAAGCTTGAGAAGGTGTTTGTATCAGAAGAAGAGCTTCTTAAGAAGATTAAAGGAAGCAAAATTCATAAGACATATAAAGCTGGCTTTTGGGAATCGATTAAATCTGGGAGAGAAGGATTGGAGGGTGATGAGCTAAGAAGGAATTCTGATGTCATGGAGATCCTAAAGAAGACAAGTTTCCAAAGGCAGATCATTAGAATGTCTACAGTTCAAACTGAGAAGCCCAGTTTATCATATGAATCCCTACCGATAAAGCCTCCTTCACATCCGACTTATGATTTGTTGGGTGTCATCAAGTCAGCCCTTGCTGAAGACACTGGGGATCGAGGTGATGTGACATGTCTGGCTACCATTCCATCTGAGATGGAAGTAGAAGCCCACTTCTTGGCAAAGGAGAATTGTATAGCTGCTAGAATTGCACTTGCAGAGATGGTATTTTATGAAGTTGATCCCACTCTAAAGGTTGTTGTTTGCAAGTAA

Protein Analysis

330

Amino Acids

37.07

Weight (kDa)

5.85

Isoelectric Point (pI)

31.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 13 - 192 1.2e-37 NmrA-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 898
AccI GTMKAC 1 cut(s) 716
AclWI GGATC 4 cut(s) 410, 667, 843, 956
AcsI RAATTY 4 cut(s) 140, 168, 573, 655
AcuI CTGAAG 1 cut(s) 843
AcyI GRCGYC 1 cut(s) 217
AfaI GTAC 1 cut(s) 467
AfiI CCNNNNNNNGG 3 cut(s) 41, 213, 898
AflII CTTAAG 1 cut(s) 551
AflIII ACRYGT 1 cut(s) 851
AgsI TTSAA 3 cut(s) 198, 430, 725
AluBI AGCT 9 cut(s) 230, 276, 389, 490, 517, 547, 593, 646, 920
AluI AGCT 9 cut(s) 230, 276, 389, 490, 517, 547, 593, 646, 920
AlwI GGATC 4 cut(s) 410, 667, 843, 956
AoxI GGCC 2 cut(s) 304, 449
ApeKI GCWGC 3 cut(s) 298, 389, 920
ApoI RAATTY 4 cut(s) 140, 168, 573, 655
ArsI GACNNNNNNTTYG 2 cut(s) 775, 807
Asp700I GAANNNNTTC 2 cut(s) 546, 655
AspS9I GGNCC 2 cut(s) 151, 304
AsuC2I CCSGG 2 cut(s) 303, 456
AsuHPI GGTGA 3 cut(s) 24, 650, 854
AvaII GGWCC 1 cut(s) 151
BauI CACGAG 1 cut(s) 5
BbsI GAAGAC 3 cut(s) 60, 689, 831
BbvI GCAGC 3 cut(s) 310, 376, 907
BccI CCATC 5 cut(s) 142, 172, 871, 877, 937
BcgI CGANNNNNNTGC 2 cut(s) 262, 296
BcnI CCSGG 2 cut(s) 303, 456
BfaI CTAG 1 cut(s) 924
BfmI CTRYAG 1 cut(s) 717
BfrI CTTAAG 1 cut(s) 551
BisI GCNGC 3 cut(s) 299, 390, 921
BlsI GCNGC 3 cut(s) 300, 391, 922
Bme1390I CCNGG 2 cut(s) 303, 456
Bme18I GGWCC 1 cut(s) 151
BmgT120I GGNCC 2 cut(s) 151, 304
BmiI GGNNCC 1 cut(s) 153
BmrFI CCNGG 2 cut(s) 303, 456
BmrI ACTGGG 2 cut(s) 732, 840
BmuI ACTGGG 2 cut(s) 732, 840
BpiI GAAGAC 3 cut(s) 60, 689, 831
BpuEI CTTGAG 2 cut(s) 216, 539
BpuMI CCSGG 2 cut(s) 303, 456
Bsa29I ATCGAT 1 cut(s) 608
BsaHI GRCGYC 1 cut(s) 217
BsaJI CCNNGG 1 cut(s) 320
Bsc4I CCNNNNNNNGG 3 cut(s) 41, 213, 898
Bse1I ACTGG 2 cut(s) 738, 835
BseCI ATCGAT 1 cut(s) 608
BseDI CCNNGG 1 cut(s) 320
BseGI GGATG 2 cut(s) 183, 778
BseLI CCNNNNNNNGG 3 cut(s) 41, 213, 898
BseMII CTCAG 2 cut(s) 720, 864
BseNI ACTGG 2 cut(s) 738, 835
BseXI GCAGC 3 cut(s) 310, 376, 907
BsgI GTGCAG 1 cut(s) 317
BshFI GGCC 2 cut(s) 306, 451
BshVI ATCGAT 1 cut(s) 608
BsiSI CCGG 2 cut(s) 302, 456
BslFI GGGAC 2 cut(s) 338, 359
BslI CCNNNNNNNGG 3 cut(s) 41, 213, 898
BsmFI GGGAC 2 cut(s) 338, 359
BsmI GAATGC 1 cut(s) 237
BsnI GGCC 2 cut(s) 306, 451
Bsp143I GATC 5 cut(s) 415, 672, 702, 835, 961
BspANI GGCC 2 cut(s) 306, 451
BspCNI CTCAG 2 cut(s) 721, 865
BspDI ATCGAT 1 cut(s) 608
BspLI GGNNCC 1 cut(s) 153
BspPI GGATC 4 cut(s) 410, 667, 843, 956
BspQI GCTCTTC 1 cut(s) 537
BspTI CTTAAG 1 cut(s) 551
BsrI ACTGG 2 cut(s) 738, 835
BssECI CCNNGG 1 cut(s) 320
BssMI GATC 5 cut(s) 415, 672, 702, 835, 961
BssNI GRCGYC 1 cut(s) 217
BssSI CACGAG 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 320
Bst2BI CACGAG 1 cut(s) 5
Bst4CI ACNGT 1 cut(s) 721
Bst6I CTCTTC 2 cut(s) 54, 537
BstACI GRCGYC 1 cut(s) 217
BstAFI CTTAAG 1 cut(s) 551
BstC8I GCNNGC 1 cut(s) 595
BstDEI CTNAG 3 cut(s) 647, 729, 873
BstF5I GGATG 2 cut(s) 183, 778
BstKTI GATC 5 cut(s) 418, 675, 705, 838, 964
BstMBI GATC 5 cut(s) 415, 672, 702, 835, 961
BstMWI GCNNNNNNNGC 1 cut(s) 929
BstNSI RCATGY 1 cut(s) 855
BstSCI CCNGG 2 cut(s) 301, 454
BstSFI CTRYAG 1 cut(s) 717
BstV1I GCAGC 3 cut(s) 310, 376, 907
BstV2I GAAGAC 3 cut(s) 60, 689, 831
BstX2I RGATCY 1 cut(s) 672
BstYI RGATCY 1 cut(s) 672
Bsu15I ATCGAT 1 cut(s) 608
BsuRI GGCC 2 cut(s) 306, 451
BsuTUI ATCGAT 1 cut(s) 608
BtsCI GGATG 2 cut(s) 183, 778
BtsI GCAGTG 1 cut(s) 84
BtsIMutI CAGTG 2 cut(s) 84, 828
Cac8I GCNNGC 1 cut(s) 595
Cfr13I GGNCC 2 cut(s) 151, 304
ClaI ATCGAT 1 cut(s) 608
CseI GACGC 2 cut(s) 172, 206
Csp6I GTAC 1 cut(s) 466
CspCI CAANNNNNGTGG 2 cut(s) 408, 443
CviAII CATG 2 cut(s) 667, 852
CviQI GTAC 1 cut(s) 466
DdeI CTNAG 3 cut(s) 647, 729, 873
DpnI GATC 5 cut(s) 417, 674, 704, 837, 963
DpnII GATC 5 cut(s) 415, 672, 702, 835, 961
Eam1104I CTCTTC 2 cut(s) 54, 537
EarI CTCTTC 2 cut(s) 54, 537
Eco130I CCWWGG 1 cut(s) 320
Eco47I GGWCC 1 cut(s) 151
Eco57I CTGAAG 1 cut(s) 843
EcoRI GAATTC 2 cut(s) 140, 655
EcoT14I CCWWGG 1 cut(s) 320
ErhI CCWWGG 1 cut(s) 320
FaeI CATG 2 cut(s) 670, 855
FalI AAGNNNNNCTT 6 cut(s) 51, 83, 531, 563, 534, 566
FaqI GGGAC 2 cut(s) 338, 359
FatI CATG 2 cut(s) 666, 851
FauNDI CATATG 1 cut(s) 748
FblI GTMKAC 1 cut(s) 716
Fnu4HI GCNGC 3 cut(s) 299, 390, 921
FokI GGATG 2 cut(s) 190, 765
Fsp4HI GCNGC 3 cut(s) 299, 390, 921
FspBI CTAG 1 cut(s) 924
GluI GCNGC 3 cut(s) 299, 390, 921
HaeIII GGCC 2 cut(s) 306, 451
HapII CCGG 2 cut(s) 302, 456
HgaI GACGC 2 cut(s) 172, 206
Hin1I GRCGYC 1 cut(s) 217
Hin1II CATG 2 cut(s) 670, 855
HindIII AAGCTT 1 cut(s) 515
HinfI GANTC 4 cut(s) 26, 126, 605, 752
HpaII CCGG 2 cut(s) 302, 456
HphI GGTGA 3 cut(s) 24, 650, 854
Hpy166II GTNNAC 2 cut(s) 466, 717
Hpy188I TCNGA 5 cut(s) 139, 538, 661, 783, 874
Hpy188III TCNNGA 2 cut(s) 334, 403
Hpy8I GTNNAC 2 cut(s) 466, 717
HpyAV CCTTC 6 cut(s) 297, 403, 517, 620, 645, 783
HpyCH4III ACNGT 1 cut(s) 721
HpyCH4V TGCA 5 cut(s) 261, 298, 932, 938, 987
HpyF10VI GCNNNNNNNGC 1 cut(s) 929
HpyF3I CTNAG 3 cut(s) 647, 729, 873
Hsp92I GRCGYC 1 cut(s) 217
Hsp92II CATG 2 cut(s) 670, 855
Kzo9I GATC 5 cut(s) 415, 672, 702, 835, 961
LguI GCTCTTC 1 cut(s) 537
LmnI GCTCC 1 cut(s) 74
Lsp1109I GCAGC 3 cut(s) 310, 376, 907
MaeI CTAG 1 cut(s) 924
MaeIII GTNAC 2 cut(s) 331, 847
MalI GATC 5 cut(s) 417, 674, 704, 837, 963
MboI GATC 5 cut(s) 415, 672, 702, 835, 961
MboII GAAGA 9 cut(s) 21, 65, 71, 523, 551, 554, 568, 694, 836
MflI RGATCY 1 cut(s) 672
MluCI AATT 8 cut(s) 140, 168, 262, 378, 573, 655, 910, 927
MmeI TCCRAC 3 cut(s) 52, 129, 806
MnlI CCTC 5 cut(s) 317, 327, 628, 780, 833
MroXI GAANNNNTTC 2 cut(s) 546, 655
MseI TTAA 4 cut(s) 39, 552, 561, 612
MslI CAYNNNNRTG 1 cut(s) 710
MspCI CTTAAG 1 cut(s) 551
MspI CCGG 2 cut(s) 302, 456
MspR9I CCNGG 2 cut(s) 303, 456
Mva1269I GAATGC 1 cut(s) 237
MwoI GCNNNNNNNGC 1 cut(s) 929
NciI CCSGG 2 cut(s) 303, 456
NdeI CATATG 1 cut(s) 748
NdeII GATC 5 cut(s) 415, 672, 702, 835, 961
NlaIII CATG 2 cut(s) 670, 855
NlaIV GGNNCC 1 cut(s) 153
NmeAIII GCCGAG 1 cut(s) 232
NmuCI GTSAC 2 cut(s) 331, 847
NspI RCATGY 1 cut(s) 855
PciI ACATGT 1 cut(s) 851
PciSI GCTCTTC 1 cut(s) 537
PctI GAATGC 1 cut(s) 237
PdmI GAANNNNTTC 2 cut(s) 546, 655
PfeI GAWTC 4 cut(s) 26, 126, 605, 752
PflFI GACNNNGTC 1 cut(s) 329
PflMI CCANNNNNTGG 1 cut(s) 898
PkrI GCNGC 3 cut(s) 300, 391, 922
PscI ACATGT 1 cut(s) 851
PspN4I GGNNCC 1 cut(s) 153
PspPI GGNCC 2 cut(s) 151, 304
PsuI RGATCY 1 cut(s) 672
PsyI GACNNNGTC 1 cut(s) 329
RsaI GTAC 1 cut(s) 467
RsaNI GTAC 1 cut(s) 466
RseI CAYNNNNRTG 1 cut(s) 710
SapI GCTCTTC 1 cut(s) 537
SaqAI TTAA 4 cut(s) 39, 552, 561, 612
SatI GCNGC 3 cut(s) 299, 390, 921
Sau3AI GATC 5 cut(s) 415, 672, 702, 835, 961
Sau96I GGNCC 2 cut(s) 151, 304
ScrFI CCNGG 2 cut(s) 303, 456
SfcI CTRYAG 1 cut(s) 717
SinI GGWCC 1 cut(s) 151
SmiMI CAYNNNNRTG 1 cut(s) 710
SmlI CTYRAG 3 cut(s) 231, 518, 551
SmoI CTYRAG 3 cut(s) 231, 518, 551
Sse9I AATT 8 cut(s) 140, 168, 262, 378, 573, 655, 910, 927
SspMI CTAG 1 cut(s) 924
StyD4I CCNGG 2 cut(s) 301, 454
StyI CCWWGG 1 cut(s) 320
TaaI ACNGT 1 cut(s) 721
TaqI TCGA 2 cut(s) 608, 838
TasI AATT 8 cut(s) 140, 168, 262, 378, 573, 655, 910, 927
TatI WGTACW 1 cut(s) 465
TfiI GAWTC 4 cut(s) 26, 126, 605, 752
Tru1I TTAA 4 cut(s) 39, 552, 561, 612
Tru9I TTAA 4 cut(s) 39, 552, 561, 612
TscAI CASTG 2 cut(s) 84, 835
TseFI GTSAC 2 cut(s) 331, 847
TseI GCWGC 3 cut(s) 298, 389, 920
Tsp45I GTSAC 2 cut(s) 331, 847
TspDTI ATGAA 6 cut(s) 32, 118, 492, 566, 765, 969
TspGWI ACGGA 1 cut(s) 363
TspRI CASTG 2 cut(s) 84, 835
Tth111I GACNNNGTC 1 cut(s) 329
Van91I CCANNNNNTGG 1 cut(s) 898
Vha464I CTTAAG 1 cut(s) 551
VpaK11BI GGWCC 1 cut(s) 151
XapI RAATTY 4 cut(s) 140, 168, 573, 655
XceI RCATGY 1 cut(s) 855
XmiI GTMKAC 1 cut(s) 716
XmnI GAANNNNTTC 2 cut(s) 546, 655
XspI CTAG 1 cut(s) 924
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.