Rw6G011700

reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0003183
Physical Location & Seq
Forward (+)
20768995 .. 20771026
2032 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G011700.1

Sequence Viewer

Length: 930 bp
ATGGAGAAGAAGGAGAGCAGAGTACTGATAATCGGAGCAACAGGAAATCTGGGTCACCATTTAGCCAATGCCAGCCTCCATTTCTCTCACCCCACTTTCGCTCTCGTCCGACCCGACACCTTCTCCGACCCCCGCAAGTCCCAAAACCTTCGAGCCCTCTCCGATGCCGGCGTCACACTCCTCCAAGGTTCGCTGGAAGATGAAGAGAGCCTTGTTGAAGCAGTGAAGCAAGTTGATGTTGTTGTTTGTGCTGTTCAGGCCAAACAGGTGCTCCATCAGAAGCTTCTTATCCAAGTTATCAAAAATGCTGGCTCCATCAAGAGATTCGTTCCATCAGAATTTGGGTTGGACCCAGATAAGACGCAGATTTCAGGGATGGACTACAACTTCTATTCAAACAAAGCCGAGATTAGGCGTCTTGTGGAAGCTCAAGGCATTCCCTACACTTTTGTTTCCTGCAATTTCTATACGAGCTATTTGCTTCCTTCACTTGTGCAGCCGGGCCTCAAAGTGCCTCCCAGGGACAAAGTCACGATTTTTGGGGATGGAAATACTAAAGGCGTTTTTGTCAAGGAAATTGATGTAGCTGCCTTCACTATCAAGACATTGGACGATCCACGAACCTTGAACAAAGTGTTGTATTTTAGGCCACCCGGAAATGTGTACTCGCTGAATGAACTTGTTGAGCTTTGGGAGAGTAAGATTGGGAAGAAGCTTGAGAAGGTGTTTGTTTCAGAAGAAGAGCTTCTTAAGAAGATTAAAGAGACTTCATATCCTGACAACATGGCCCTGATTTTCATATATTCTGCTTTCATAAAGGGCGATCAAACTTACTTTGATATCGAGTCAGCTGATAGTGCCAATGCGACAAAACTGTATCCAGAGCTGAATTATACTACAATCAGTCAATATTTAGACACTCTATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0010283 GO:0016491 GO:0016627 GO:0016628 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NmrA

Protein Analysis

309

Amino Acids

34.64

Weight (kDa)

6.02

Isoelectric Point (pI)

28.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 5 - 305 3.6e-82 NmrA-like family
NAD_binding_10 PF13460 12 - 206 3.6e-16 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 133
AclWI GGATC 1 cut(s) 608
AcsI RAATTY 1 cut(s) 338
AcyI GRCGYC 2 cut(s) 171, 415
AfaI GTAC 2 cut(s) 24, 665
AfiI CCNNNNNNNGG 1 cut(s) 411
AflII CTTAAG 1 cut(s) 749
AgsI TTSAA 3 cut(s) 218, 396, 628
AjnI CCWGG 1 cut(s) 518
AluBI AGCT 9 cut(s) 283, 428, 474, 587, 688, 715, 745, 851, 886
AluI AGCT 9 cut(s) 283, 428, 474, 587, 688, 715, 745, 851, 886
Alw21I GWGCWC 1 cut(s) 273
Alw26I GTCTC 1 cut(s) 758
AlwI GGATC 1 cut(s) 608
AoxI GGCC 4 cut(s) 258, 502, 647, 786
ApeKI GCWGC 2 cut(s) 496, 587
ApoI RAATTY 1 cut(s) 338
Asp700I GAANNNNTTC 1 cut(s) 744
AspS9I GGNCC 3 cut(s) 349, 502, 787
AsuC2I CCSGG 2 cut(s) 501, 654
AsuHPI GGTGA 2 cut(s) 47, 80
AvaII GGWCC 1 cut(s) 349
BanII GRGCYC 1 cut(s) 157
Bbv12I GWGCWC 1 cut(s) 273
BbvI GCAGC 2 cut(s) 508, 574
BccI CCATC 5 cut(s) 282, 323, 340, 370, 539
BcgI CGANNNNNNTGC 2 cut(s) 460, 494
BciT130I CCWGG 1 cut(s) 520
BciVI GTATCC 1 cut(s) 888
BcnI CCSGG 2 cut(s) 501, 654
BcoDI GTCTC 1 cut(s) 758
BfrI CTTAAG 1 cut(s) 749
BfuI GTATCC 1 cut(s) 888
BisI GCNGC 2 cut(s) 497, 588
BlsI GCNGC 2 cut(s) 498, 589
BmcAI AGTACT 1 cut(s) 24
Bme1390I CCNGG 3 cut(s) 501, 520, 654
Bme18I GGWCC 1 cut(s) 349
BmgT120I GGNCC 3 cut(s) 349, 502, 787
BmiI GGNNCC 2 cut(s) 313, 351
BmrFI CCNGG 3 cut(s) 501, 520, 654
BmsI GCATC 1 cut(s) 154
BpuEI CTTGAG 2 cut(s) 414, 737
BpuMI CCSGG 2 cut(s) 501, 654
BsaHI GRCGYC 2 cut(s) 171, 415
BsaJI CCNNGG 3 cut(s) 184, 518, 519
BsaXI ACNNNNNCTCC 4 cut(s) 107, 137, 255, 285
Bsc4I CCNNNNNNNGG 1 cut(s) 411
Bse118I RCCGGY 1 cut(s) 167
BseBI CCWGG 1 cut(s) 520
BseDI CCNNGG 3 cut(s) 184, 518, 519
BseGI GGATG 2 cut(s) 381, 550
BseLI CCNNNNNNNGG 1 cut(s) 411
BseRI GAGGAG 1 cut(s) 170
BseXI GCAGC 2 cut(s) 508, 574
BsgI GTGCAG 1 cut(s) 515
BshFI GGCC 4 cut(s) 260, 504, 649, 788
BsiHKAI GWGCWC 1 cut(s) 273
BsiSI CCGG 3 cut(s) 168, 500, 654
BslFI GGGAC 2 cut(s) 124, 536
BslI CCNNNNNNNGG 1 cut(s) 411
BsmAI GTCTC 1 cut(s) 758
BsmFI GGGAC 2 cut(s) 124, 536
BsmI GAATGC 1 cut(s) 435
BsnI GGCC 4 cut(s) 260, 504, 649, 788
Bsp1286I GDGCHC 2 cut(s) 157, 273
Bsp143I GATC 2 cut(s) 613, 823
BspACI CCGC 1 cut(s) 133
BspANI GGCC 4 cut(s) 260, 504, 649, 788
BspLI GGNNCC 2 cut(s) 313, 351
BspPI GGATC 1 cut(s) 608
BspQI GCTCTTC 1 cut(s) 735
BspTI CTTAAG 1 cut(s) 749
BsrFI RCCGGY 1 cut(s) 167
BssAI RCCGGY 1 cut(s) 167
BssECI CCNNGG 3 cut(s) 184, 518, 519
BssMI GATC 2 cut(s) 613, 823
BssNI GRCGYC 2 cut(s) 171, 415
BssT1I CCWWGG 1 cut(s) 184
Bst2UI CCWGG 1 cut(s) 520
Bst4CI ACNGT 1 cut(s) 876
Bst6I CTCTTC 2 cut(s) 198, 735
BstACI GRCGYC 2 cut(s) 171, 415
BstAFI CTTAAG 1 cut(s) 749
BstC8I GCNNGC 3 cut(s) 73, 169, 310
BstEII GGTNACC 1 cut(s) 53
BstF5I GGATG 2 cut(s) 381, 550
BstKTI GATC 2 cut(s) 616, 826
BstMAI GTCTC 1 cut(s) 758
BstMBI GATC 2 cut(s) 613, 823
BstMWI GCNNNNNNNGC 2 cut(s) 257, 857
BstNI CCWGG 1 cut(s) 520
BstPI GGTNACC 1 cut(s) 53
BstSCI CCNGG 3 cut(s) 499, 518, 652
BstV1I GCAGC 2 cut(s) 508, 574
BsuI GTATCC 1 cut(s) 888
BsuRI GGCC 4 cut(s) 260, 504, 649, 788
BtsCI GGATG 2 cut(s) 381, 550
BtsI GCAGTG 1 cut(s) 228
BtsIMutI CAGTG 1 cut(s) 228
Cac8I GCNNGC 3 cut(s) 73, 169, 310
Cfr10I RCCGGY 1 cut(s) 167
Cfr13I GGNCC 3 cut(s) 349, 502, 787
CseI GACGC 3 cut(s) 160, 370, 404
Csp6I GTAC 2 cut(s) 23, 664
CspCI CAANNNNNGTGG 2 cut(s) 606, 641
CviAII CATG 1 cut(s) 784
CviQI GTAC 2 cut(s) 23, 664
DpnI GATC 2 cut(s) 615, 825
DpnII GATC 2 cut(s) 613, 823
Eam1104I CTCTTC 2 cut(s) 198, 735
EarI CTCTTC 2 cut(s) 198, 735
Eco130I CCWWGG 1 cut(s) 184
Eco24I GRGCYC 1 cut(s) 157
Eco32I GATATC 1 cut(s) 841
Eco47I GGWCC 1 cut(s) 349
Eco91I GGTNACC 1 cut(s) 53
EcoO65I GGTNACC 1 cut(s) 53
EcoRII CCWGG 1 cut(s) 518
EcoRV GATATC 1 cut(s) 841
EcoT14I CCWWGG 1 cut(s) 184
EcoT38I GRGCYC 1 cut(s) 157
ErhI CCWWGG 1 cut(s) 184
FaeI CATG 1 cut(s) 787
FaiI YATR 7 cut(s) 468, 772, 785, 800, 802, 815, 894
FalI AAGNNNNNCTT 6 cut(s) 195, 227, 729, 761, 732, 764
FaqI GGGAC 2 cut(s) 124, 536
FatI CATG 1 cut(s) 783
FauI CCCGC 1 cut(s) 140
Fnu4HI GCNGC 2 cut(s) 497, 588
FokI GGATG 2 cut(s) 388, 557
FriOI GRGCYC 1 cut(s) 157
Fsp4HI GCNGC 2 cut(s) 497, 588
GluI GCNGC 2 cut(s) 497, 588
HaeIII GGCC 4 cut(s) 260, 504, 649, 788
HapII CCGG 3 cut(s) 168, 500, 654
HgaI GACGC 3 cut(s) 160, 370, 404
Hin1I GRCGYC 2 cut(s) 171, 415
Hin1II CATG 1 cut(s) 787
HindIII AAGCTT 2 cut(s) 281, 713
HinfI GANTC 2 cut(s) 324, 845
HpaII CCGG 3 cut(s) 168, 500, 654
HphI GGTGA 2 cut(s) 47, 80
Hpy166II GTNNAC 1 cut(s) 664
Hpy188I TCNGA 7 cut(s) 35, 110, 127, 163, 279, 337, 736
Hpy188III TCNNGA 5 cut(s) 319, 532, 601, 776, 881
Hpy8I GTNNAC 1 cut(s) 664
HpyAV CCTTC 6 cut(s) 4, 130, 158, 495, 601, 715
HpyCH4III ACNGT 1 cut(s) 876
HpyCH4V TGCA 2 cut(s) 459, 496
HpyF10VI GCNNNNNNNGC 2 cut(s) 257, 857
Hsp92I GRCGYC 2 cut(s) 171, 415
Hsp92II CATG 1 cut(s) 787
KroI GCCGGC 1 cut(s) 167
KroNI GCCGGC 1 cut(s) 169
Kzo9I GATC 2 cut(s) 613, 823
LguI GCTCTTC 1 cut(s) 735
LmnI GCTCC 3 cut(s) 35, 276, 317
Lsp1109I GCAGC 2 cut(s) 508, 574
LweI GCATC 1 cut(s) 154
MaeIII GTNAC 3 cut(s) 53, 172, 529
MalI GATC 2 cut(s) 615, 825
MboI GATC 2 cut(s) 613, 823
MboII GAAGA 7 cut(s) 19, 209, 215, 721, 749, 752, 766
MhlI GDGCHC 2 cut(s) 157, 273
MluCI AATT 4 cut(s) 338, 460, 576, 889
MlyI GAGTC 1 cut(s) 854
MmeI TCCRAC 3 cut(s) 133, 150, 327
MnlI CCTC 5 cut(s) 86, 167, 191, 515, 525
MroNI GCCGGC 1 cut(s) 167
MroXI GAANNNNTTC 1 cut(s) 744
MseI TTAA 2 cut(s) 750, 759
MspA1I CMGCKG 1 cut(s) 851
MspCI CTTAAG 1 cut(s) 749
MspI CCGG 3 cut(s) 168, 500, 654
MspR9I CCNGG 3 cut(s) 501, 520, 654
Mva1269I GAATGC 1 cut(s) 435
MvaI CCWGG 1 cut(s) 520
MwoI GCNNNNNNNGC 2 cut(s) 257, 857
NaeI GCCGGC 1 cut(s) 169
NciI CCSGG 2 cut(s) 501, 654
NdeII GATC 2 cut(s) 613, 823
NgoMIV GCCGGC 1 cut(s) 167
NlaIII CATG 1 cut(s) 787
NlaIV GGNNCC 2 cut(s) 313, 351
NmeAIII GCCGAG 1 cut(s) 430
NmuCI GTSAC 3 cut(s) 53, 172, 529
PasI CCCWGGG 1 cut(s) 519
PciSI GCTCTTC 1 cut(s) 735
PcsI WCGNNNNNNNCGW 1 cut(s) 111
PctI GAATGC 1 cut(s) 435
PdiI GCCGGC 1 cut(s) 169
PdmI GAANNNNTTC 1 cut(s) 744
PfeI GAWTC 1 cut(s) 324
PflFI GACNNNGTC 1 cut(s) 527
PkrI GCNGC 2 cut(s) 498, 589
PleI GAGTC 1 cut(s) 853
PpsI GAGTC 1 cut(s) 853
Psp6I CCWGG 1 cut(s) 518
PspEI GGTNACC 1 cut(s) 53
PspGI CCWGG 1 cut(s) 518
PspN4I GGNNCC 2 cut(s) 313, 351
PspPI GGNCC 3 cut(s) 349, 502, 787
PsyI GACNNNGTC 1 cut(s) 527
PvuII CAGCTG 1 cut(s) 851
RsaI GTAC 2 cut(s) 24, 665
RsaNI GTAC 2 cut(s) 23, 664
SapI GCTCTTC 1 cut(s) 735
SaqAI TTAA 2 cut(s) 750, 759
SatI GCNGC 2 cut(s) 497, 588
Sau3AI GATC 2 cut(s) 613, 823
Sau96I GGNCC 3 cut(s) 349, 502, 787
ScaI AGTACT 1 cut(s) 24
SchI GAGTC 1 cut(s) 854
ScrFI CCNGG 3 cut(s) 501, 520, 654
SduI GDGCHC 2 cut(s) 157, 273
SfaNI GCATC 1 cut(s) 154
SinI GGWCC 1 cut(s) 349
SmlI CTYRAG 3 cut(s) 429, 716, 749
SmoI CTYRAG 3 cut(s) 429, 716, 749
Sse9I AATT 4 cut(s) 338, 460, 576, 889
SsiI CCGC 1 cut(s) 133
SspI AATATT 1 cut(s) 911
StyD4I CCNGG 3 cut(s) 499, 518, 652
StyI CCWWGG 1 cut(s) 184
TaaI ACNGT 1 cut(s) 876
TaqI TCGA 2 cut(s) 151, 843
TasI AATT 4 cut(s) 338, 460, 576, 889
TatI WGTACW 2 cut(s) 22, 663
TfiI GAWTC 1 cut(s) 324
Tru1I TTAA 2 cut(s) 750, 759
Tru9I TTAA 2 cut(s) 750, 759
TscAI CASTG 1 cut(s) 228
TseFI GTSAC 3 cut(s) 53, 172, 529
TseI GCWGC 2 cut(s) 496, 587
Tsp45I GTSAC 3 cut(s) 53, 172, 529
TspDTI ATGAA 5 cut(s) 216, 690, 759, 787, 802
TspRI CASTG 1 cut(s) 228
Tth111I GACNNNGTC 1 cut(s) 527
Vha464I CTTAAG 1 cut(s) 749
VpaK11BI GGWCC 1 cut(s) 349
XapI RAATTY 1 cut(s) 338
XmnI GAANNNNTTC 1 cut(s) 744
ZrmI AGTACT 1 cut(s) 24
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.