Rh6AG179200

isoflavone

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0003183
Physical Location & Seq
Reverse (-)
30946116 .. 30955739
9624 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG179200.1

Sequence Viewer

Length: 867 bp
ATGGAGAAGAAGAAGAGTAGAGTACTGATAATCAGAGCAACAGGAAATCTGGGTCACCATTTAGCCAATGCCAGCCTCCATTTCTCTCACCCCACTTTCGCTCTCGTCCGACCCGACACCTTCTCCGACCCCCACAAGTCCCAAAACCTTCGAGCCCTCTCTGATGCCGGCGTCACACTCCTCCAAGTCGGTTCGCTGGAAGACGAAGAGAACCTTGTTGGAGCAGTGAAGCAAGTTGATGTTGTGATTTGTGCTGTTCAGGTCAAACAGAGATTCATTCCATCAGAATTCGGGTTGGACCCAGATAAGACGCAAATTTCAGGGACGGACTACAACTTCTATTCAAACAAAGCCGAGATTAGGCGTCTTGTGGAAGCTCAAGGCATTCCCTACACTTTTATTTCCTGCAATTTCTATACGAGCTATTTGCTTCCTTCACTTGTGCAGCCGGGCCTCAAAGTGCCTCCCAGGGACAAAGTCACGATTTTCGGGGACAGAAATACTAAAGGCGTTTTTGTAAAGGAAATTGATGTAGCTGCCTTCACTATCAGGACATTGGACGATCCACGAACCTTGAACAAAGTGTTGTATTTTAGGCCACCCGGAAATGTGTACTCGCTGAATGAACTTGTTGAGCTTTGGGAGAGTAAGATTGGGAAGAAGCTTGAGAAGGTGTTTGTATCAGAAGAAGAGCTTCTTAAGAAGATTAAAGGAAGCAAAATTCATAAGACATATAAAGCTGGCTTTTGGGAATCGATTAAACCTGGGAGAGAAGGATTGGAGGGTGATGAGCTAAGAAGGAATTCTGATGTCATGGAGATCCTAAAGAAGACAAGTTTCCAAAGGTTATTGGCTCTAGCGCGTTGA

Protein Analysis

288

Amino Acids

32.54

Weight (kDa)

9.3

Isoelectric Point (pI)

29.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 4 - 241 1.1e-51 NmrA-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 862
AclWI GGATC 2 cut(s) 557, 814
AcsI RAATTY 4 cut(s) 287, 315, 720, 802
AcyI GRCGYC 2 cut(s) 171, 364
AfaI GTAC 2 cut(s) 24, 614
AfiI CCNNNNNNNGG 1 cut(s) 360
AflII CTTAAG 1 cut(s) 698
AgsI TTSAA 2 cut(s) 345, 577
AjnI CCWGG 2 cut(s) 467, 763
AluBI AGCT 8 cut(s) 377, 423, 536, 637, 664, 694, 740, 793
AluI AGCT 8 cut(s) 377, 423, 536, 637, 664, 694, 740, 793
AlwI GGATC 2 cut(s) 557, 814
AoxI GGCC 2 cut(s) 451, 596
ApeKI GCWGC 2 cut(s) 445, 536
ApoI RAATTY 4 cut(s) 287, 315, 720, 802
Asp700I GAANNNNTTC 2 cut(s) 693, 802
AspLEI GCGC 1 cut(s) 862
AspS9I GGNCC 2 cut(s) 298, 451
AsuC2I CCSGG 2 cut(s) 450, 603
AsuHPI GGTGA 3 cut(s) 47, 80, 797
AvaII GGWCC 1 cut(s) 298
BanII GRGCYC 1 cut(s) 157
BbsI GAAGAC 2 cut(s) 207, 836
BbvI GCAGC 2 cut(s) 457, 523
BccI CCATC 1 cut(s) 289
BcgI CGANNNNNNTGC 2 cut(s) 409, 443
BciT130I CCWGG 2 cut(s) 469, 765
BcnI CCSGG 2 cut(s) 450, 603
BfaI CTAG 1 cut(s) 857
BfrI CTTAAG 1 cut(s) 698
BisI GCNGC 2 cut(s) 446, 537
BlsI GCNGC 2 cut(s) 447, 538
BmcAI AGTACT 1 cut(s) 24
Bme1390I CCNGG 4 cut(s) 450, 469, 603, 765
Bme18I GGWCC 1 cut(s) 298
BmgT120I GGNCC 2 cut(s) 298, 451
BmiI GGNNCC 1 cut(s) 300
BmrFI CCNGG 4 cut(s) 450, 469, 603, 765
BmsI GCATC 1 cut(s) 154
BpiI GAAGAC 2 cut(s) 207, 836
BpuEI CTTGAG 2 cut(s) 363, 686
BpuMI CCSGG 2 cut(s) 450, 603
Bsa29I ATCGAT 1 cut(s) 755
BsaHI GRCGYC 2 cut(s) 171, 364
BsaJI CCNNGG 3 cut(s) 467, 468, 764
BsaXI ACNNNNNCTCC 2 cut(s) 107, 137
Bsc4I CCNNNNNNNGG 1 cut(s) 360
Bse118I RCCGGY 1 cut(s) 167
BseBI CCWGG 2 cut(s) 469, 765
BseCI ATCGAT 1 cut(s) 755
BseDI CCNNGG 3 cut(s) 467, 468, 764
BseLI CCNNNNNNNGG 1 cut(s) 360
BseRI GAGGAG 1 cut(s) 170
BseXI GCAGC 2 cut(s) 457, 523
BsgI GTGCAG 1 cut(s) 464
Bsh1236I CGCG 1 cut(s) 862
BshFI GGCC 2 cut(s) 453, 598
BshVI ATCGAT 1 cut(s) 755
BsiSI CCGG 3 cut(s) 168, 449, 603
BslFI GGGAC 4 cut(s) 124, 337, 485, 506
BslI CCNNNNNNNGG 1 cut(s) 360
BsmFI GGGAC 4 cut(s) 124, 337, 485, 506
BsmI GAATGC 1 cut(s) 384
BsnI GGCC 2 cut(s) 453, 598
Bsp1286I GDGCHC 1 cut(s) 157
Bsp143I GATC 2 cut(s) 562, 819
BspANI GGCC 2 cut(s) 453, 598
BspDI ATCGAT 1 cut(s) 755
BspFNI CGCG 1 cut(s) 862
BspLI GGNNCC 1 cut(s) 300
BspPI GGATC 2 cut(s) 557, 814
BspQI GCTCTTC 1 cut(s) 684
BspTI CTTAAG 1 cut(s) 698
BsrFI RCCGGY 1 cut(s) 167
BssAI RCCGGY 1 cut(s) 167
BssECI CCNNGG 3 cut(s) 467, 468, 764
BssMI GATC 2 cut(s) 562, 819
BssNI GRCGYC 2 cut(s) 171, 364
Bst2UI CCWGG 2 cut(s) 469, 765
Bst6I CTCTTC 3 cut(s) 8, 201, 684
BstACI GRCGYC 2 cut(s) 171, 364
BstAFI CTTAAG 1 cut(s) 698
BstC8I GCNNGC 3 cut(s) 73, 169, 742
BstDEI CTNAG 1 cut(s) 794
BstEII GGTNACC 1 cut(s) 53
BstFNI CGCG 1 cut(s) 862
BstHHI GCGC 1 cut(s) 862
BstKTI GATC 2 cut(s) 565, 822
BstMBI GATC 2 cut(s) 562, 819
BstNI CCWGG 2 cut(s) 469, 765
BstPI GGTNACC 1 cut(s) 53
BstSCI CCNGG 4 cut(s) 448, 467, 601, 763
BstUI CGCG 1 cut(s) 862
BstV1I GCAGC 2 cut(s) 457, 523
BstV2I GAAGAC 2 cut(s) 207, 836
BstX2I RGATCY 1 cut(s) 819
BstYI RGATCY 1 cut(s) 819
Bsu15I ATCGAT 1 cut(s) 755
BsuRI GGCC 2 cut(s) 453, 598
BsuTUI ATCGAT 1 cut(s) 755
BtsI GCAGTG 1 cut(s) 231
BtsIMutI CAGTG 1 cut(s) 231
Cac8I GCNNGC 3 cut(s) 73, 169, 742
CfoI GCGC 1 cut(s) 862
Cfr10I RCCGGY 1 cut(s) 167
Cfr13I GGNCC 2 cut(s) 298, 451
ClaI ATCGAT 1 cut(s) 755
CseI GACGC 3 cut(s) 160, 319, 353
Csp6I GTAC 2 cut(s) 23, 613
CspCI CAANNNNNGTGG 2 cut(s) 555, 590
CviAII CATG 1 cut(s) 814
CviQI GTAC 2 cut(s) 23, 613
DdeI CTNAG 1 cut(s) 794
DpnI GATC 2 cut(s) 564, 821
DpnII GATC 2 cut(s) 562, 819
Eam1104I CTCTTC 3 cut(s) 8, 201, 684
EarI CTCTTC 3 cut(s) 8, 201, 684
Eco24I GRGCYC 1 cut(s) 157
Eco47I GGWCC 1 cut(s) 298
Eco91I GGTNACC 1 cut(s) 53
EcoO65I GGTNACC 1 cut(s) 53
EcoRI GAATTC 2 cut(s) 287, 802
EcoRII CCWGG 2 cut(s) 467, 763
EcoT38I GRGCYC 1 cut(s) 157
FaeI CATG 1 cut(s) 817
FaiI YATR 5 cut(s) 417, 726, 733, 735, 815
FalI AAGNNNNNCTT 6 cut(s) 198, 230, 678, 710, 681, 713
FaqI GGGAC 4 cut(s) 124, 337, 485, 506
FatI CATG 1 cut(s) 813
Fnu4HI GCNGC 2 cut(s) 446, 537
FriOI GRGCYC 1 cut(s) 157
Fsp4HI GCNGC 2 cut(s) 446, 537
FspBI CTAG 1 cut(s) 857
GlaI GCGC 1 cut(s) 861
GluI GCNGC 2 cut(s) 446, 537
HaeIII GGCC 2 cut(s) 453, 598
HapII CCGG 3 cut(s) 168, 449, 603
HgaI GACGC 3 cut(s) 160, 319, 353
HhaI GCGC 1 cut(s) 862
Hin1I GRCGYC 2 cut(s) 171, 364
Hin1II CATG 1 cut(s) 817
Hin6I GCGC 1 cut(s) 860
HinP1I GCGC 1 cut(s) 860
HindIII AAGCTT 1 cut(s) 662
HinfI GANTC 2 cut(s) 273, 752
HpaII CCGG 3 cut(s) 168, 449, 603
HphI GGTGA 3 cut(s) 47, 80, 797
Hpy166II GTNNAC 1 cut(s) 613
Hpy188I TCNGA 7 cut(s) 35, 110, 127, 163, 286, 685, 808
Hpy188III TCNNGA 2 cut(s) 481, 550
Hpy8I GTNNAC 1 cut(s) 613
HpyAV CCTTC 7 cut(s) 130, 158, 444, 550, 664, 767, 792
HpyCH4V TGCA 2 cut(s) 408, 445
HpyF3I CTNAG 1 cut(s) 794
Hsp92I GRCGYC 2 cut(s) 171, 364
Hsp92II CATG 1 cut(s) 817
HspAI GCGC 1 cut(s) 860
KroI GCCGGC 1 cut(s) 167
KroNI GCCGGC 1 cut(s) 169
Kzo9I GATC 2 cut(s) 562, 819
LguI GCTCTTC 1 cut(s) 684
LmnI GCTCC 1 cut(s) 221
Lsp1109I GCAGC 2 cut(s) 457, 523
LweI GCATC 1 cut(s) 154
MaeI CTAG 1 cut(s) 857
MaeIII GTNAC 3 cut(s) 53, 172, 478
MalI GATC 2 cut(s) 564, 821
MboI GATC 2 cut(s) 562, 819
MflI RGATCY 1 cut(s) 819
MhlI GDGCHC 1 cut(s) 157
MluCI AATT 6 cut(s) 287, 315, 409, 525, 720, 802
MmeI TCCRAC 4 cut(s) 133, 150, 199, 276
MnlI CCTC 6 cut(s) 86, 167, 191, 464, 474, 775
MroNI GCCGGC 1 cut(s) 167
MroXI GAANNNNTTC 2 cut(s) 693, 802
MseI TTAA 3 cut(s) 699, 708, 759
MspCI CTTAAG 1 cut(s) 698
MspI CCGG 3 cut(s) 168, 449, 603
MspR9I CCNGG 4 cut(s) 450, 469, 603, 765
Mva1269I GAATGC 1 cut(s) 384
MvaI CCWGG 2 cut(s) 469, 765
MvnI CGCG 1 cut(s) 862
NaeI GCCGGC 1 cut(s) 169
NciI CCSGG 2 cut(s) 450, 603
NdeII GATC 2 cut(s) 562, 819
NgoMIV GCCGGC 1 cut(s) 167
NlaIII CATG 1 cut(s) 817
NlaIV GGNNCC 1 cut(s) 300
NmeAIII GCCGAG 1 cut(s) 379
NmuCI GTSAC 3 cut(s) 53, 172, 478
PasI CCCWGGG 1 cut(s) 468
PciSI GCTCTTC 1 cut(s) 684
PcsI WCGNNNNNNNCGW 1 cut(s) 111
PctI GAATGC 1 cut(s) 384
PdiI GCCGGC 1 cut(s) 169
PdmI GAANNNNTTC 2 cut(s) 693, 802
PfeI GAWTC 2 cut(s) 273, 752
PflFI GACNNNGTC 1 cut(s) 476
PkrI GCNGC 2 cut(s) 447, 538
Psp6I CCWGG 2 cut(s) 467, 763
PspEI GGTNACC 1 cut(s) 53
PspGI CCWGG 2 cut(s) 467, 763
PspN4I GGNNCC 1 cut(s) 300
PspPI GGNCC 2 cut(s) 298, 451
PsuI RGATCY 1 cut(s) 819
PsyI GACNNNGTC 1 cut(s) 476
RsaI GTAC 2 cut(s) 24, 614
RsaNI GTAC 2 cut(s) 23, 613
SapI GCTCTTC 1 cut(s) 684
SaqAI TTAA 3 cut(s) 699, 708, 759
SatI GCNGC 2 cut(s) 446, 537
Sau3AI GATC 2 cut(s) 562, 819
Sau96I GGNCC 2 cut(s) 298, 451
ScaI AGTACT 1 cut(s) 24
ScrFI CCNGG 4 cut(s) 450, 469, 603, 765
SduI GDGCHC 1 cut(s) 157
SfaNI GCATC 1 cut(s) 154
SinI GGWCC 1 cut(s) 298
SmlI CTYRAG 3 cut(s) 378, 665, 698
SmoI CTYRAG 3 cut(s) 378, 665, 698
Sse9I AATT 6 cut(s) 287, 315, 409, 525, 720, 802
SspMI CTAG 1 cut(s) 857
StyD4I CCNGG 4 cut(s) 448, 467, 601, 763
TaqI TCGA 2 cut(s) 151, 755
TasI AATT 6 cut(s) 287, 315, 409, 525, 720, 802
TatI WGTACW 2 cut(s) 22, 612
TfiI GAWTC 2 cut(s) 273, 752
Tru1I TTAA 3 cut(s) 699, 708, 759
Tru9I TTAA 3 cut(s) 699, 708, 759
TscAI CASTG 1 cut(s) 231
TseFI GTSAC 3 cut(s) 53, 172, 478
TseI GCWGC 2 cut(s) 445, 536
Tsp45I GTSAC 3 cut(s) 53, 172, 478
TspDTI ATGAA 3 cut(s) 265, 639, 713
TspGWI ACGGA 1 cut(s) 341
TspRI CASTG 1 cut(s) 231
Tth111I GACNNNGTC 1 cut(s) 476
Vha464I CTTAAG 1 cut(s) 698
VpaK11BI GGWCC 1 cut(s) 298
XapI RAATTY 4 cut(s) 287, 315, 720, 802
XmnI GAANNNNTTC 2 cut(s) 693, 802
XspI CTAG 1 cut(s) 857
ZrmI AGTACT 1 cut(s) 24
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.