RLG00000018282

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0005205
Physical Location & Seq
Forward (+)
27893291 .. 27897367
4077 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018282

Sequence Viewer

Length: 1164 bp
ATGGATATTGTCTTCTGGAATATTAGGGGCACTAAGGACCCTCGTATGTTACGGGTGTTGTCATCCTTGGTCTTCCAAAGGCGTCCTGGAGTCCTTTTCCTATTGGAGACAAAGTGTAGAAGTCAAGAATGCTTCAACTCCTTTCGAGCCAATTTAGGGTTTCAACATGAGGTCATGCAGGAGTGTAGTGACAATGGAGGGGGTGGACTGATGCTGTTCTGGGGTGAGGACATTGATCTTCATCTTTGCTCATACTCCCCACACTTTGTGGATGCAAATGTGATATTGCTGGTGTCTGAAGGGGGTCGATGCCAATTCACTGGTTTTTACGGGTATCCAGAGACGGAGCAACGTCATGTCACATGGGATTTACTCCGGCAACTTTCCATAGTATTCGGTCACCTAGATGAGATTCTTTCCTCCTCTGAGAAATATGGAGGAGCTGCACATCCTCCTTGGCAGATTGATGCCTTCCAAGCGGCTCTTGACGATTGTGGACTTGGAAGTGGAAGAGCATCTCTTCAGGGACTGTGCAGTATCGAGAAGGATCCTCACTCCTTTACTTGGACAATTGGCTCAGCGACAACTTCCTTTTCAAGCTTGGTTGAAGCTTTGCATAGGAGTGCTATCTTCTGCCCAATGGCTAATCGTCCTCCAGTTCAGATTGGTCGTCAAAGGTCTAAATGGATGAAACCGTTGCCAGGGTGGGTGAGTATTTGCTTGGATGGTGCTTTTAATTCTCTTACTTGGGAGGGGGGTGTGGGGGTTGTTGCTCGAGATAGCAATGGGACATGTATCGATGGACGGGCAAAATACCTCCAGGGTGTCTCTTCAGCTGAGCACGTTGAGTTATTAGCGGGTTTTGAAGCTTTGGAGTTGGCGCGGGATATGGGTTTGCAAGCGGTAAAGTTTGAGTTTGATTGTCTGCAGTTGGTTGGTTCGATAGCTCTTCCTTTGGAGTCTCAACTTGGCCTTCGTTTGCTTTACATGGATTTACAGTCCTTCCTATCTCAATTACCTGGTTGTTATTTTAAACATATTTATAGGGAGAAGAATGTAGTTGCTCATTACTTAGCTGGGTGGGCACTTCGTTCAAAAGTTTATTCTTCTTTTACTTCTCCTCCAAGTTTCTTGGTGGATTTGCTATCAACTGATTGTACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3

Protein Analysis

388

Amino Acids

43.03

Weight (kDa)

5.77

Isoelectric Point (pI)

47.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 242 - 361 2.5e-21 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 883
AciI CCGC 4 cut(s) 479, 857, 883, 902
AclWI GGATC 2 cut(s) 542, 555
AcuI CTGAAG 3 cut(s) 318, 506, 816
AcyI GRCGYC 1 cut(s) 82
AdeI CACNNNGTG 1 cut(s) 268
AfaI GTAC 1 cut(s) 1159
AfiI CCNNNNNNNGG 3 cut(s) 156, 564, 701
AflIII ACRYGT 1 cut(s) 791
AgsI TTSAA 6 cut(s) 136, 164, 597, 608, 866, 1095
AjnI CCWGG 4 cut(s) 85, 700, 819, 1018
AluBI AGCT 7 cut(s) 443, 600, 611, 836, 869, 947, 1076
AluI AGCT 7 cut(s) 443, 600, 611, 836, 869, 947, 1076
Alw21I GWGCWC 1 cut(s) 843
Alw26I GTCTC 4 cut(s) 101, 335, 832, 966
AlwI GGATC 2 cut(s) 542, 555
AlwNI CAGNNNCTG 1 cut(s) 529
Ama87I CYCGRG 1 cut(s) 774
AoxI GGCC 1 cut(s) 970
ApeKI GCWGC 1 cut(s) 443
AspLEI GCGC 1 cut(s) 883
AspS9I GGNCC 1 cut(s) 37
AsuHPI GGTGA 3 cut(s) 236, 392, 721
AvaI CYCGRG 1 cut(s) 774
AvaII GGWCC 1 cut(s) 37
BaeGI GKGCMC 2 cut(s) 32, 1087
BamHI GGATCC 1 cut(s) 547
BbsI GAAGAC 2 cut(s) 4, 64
Bbv12I GWGCWC 1 cut(s) 843
BbvI GCAGC 1 cut(s) 430
BccI CCATC 2 cut(s) 719, 794
BciT130I CCWGG 4 cut(s) 87, 702, 821, 1020
BciVI GTATCC 1 cut(s) 345
BcoDI GTCTC 4 cut(s) 101, 335, 832, 966
BfaI CTAG 1 cut(s) 404
BfmI CTRYAG 1 cut(s) 926
BfuI GTATCC 1 cut(s) 345
BisI GCNGC 2 cut(s) 444, 480
BlpI GCTNAGC 2 cut(s) 577, 837
BlsI GCNGC 2 cut(s) 445, 481
Bme1390I CCNGG 4 cut(s) 87, 702, 821, 1020
Bme18I GGWCC 1 cut(s) 37
BmeT110I CYCGRG 1 cut(s) 774
BmgT120I GGNCC 1 cut(s) 37
BmiI GGNNCC 2 cut(s) 39, 549
BmrFI CCNGG 4 cut(s) 87, 702, 821, 1020
BmsI GCATC 5 cut(s) 201, 262, 299, 457, 524
BpiI GAAGAC 2 cut(s) 4, 64
BpmI CTGGAG 3 cut(s) 108, 639, 803
Bpu1102I GCTNAGC 2 cut(s) 577, 837
Bsa29I ATCGAT 1 cut(s) 798
BsaBI GATNNNNATC 1 cut(s) 240
BsaHI GRCGYC 1 cut(s) 82
BsaJI CCNNGG 4 cut(s) 66, 455, 701, 820
BsaXI ACNNNNNCTCC 4 cut(s) 98, 128, 1105, 1135
Bsc4I CCNNNNNNNGG 3 cut(s) 156, 564, 701
Bse1I ACTGG 2 cut(s) 325, 656
Bse3DI GCAATG 1 cut(s) 790
Bse8I GATNNNNATC 1 cut(s) 240
BseBI CCWGG 4 cut(s) 87, 702, 821, 1020
BseCI ATCGAT 1 cut(s) 798
BseDI CCNNGG 4 cut(s) 66, 455, 701, 820
BseGI GGATG 5 cut(s) 62, 277, 448, 693, 730
BseJI GATNNNNATC 1 cut(s) 240
BseLI CCNNNNNNNGG 3 cut(s) 156, 564, 701
BseMI GCAATG 1 cut(s) 790
BseMII CTCAG 3 cut(s) 417, 591, 828
BseNI ACTGG 2 cut(s) 325, 656
BseRI GAGGAG 3 cut(s) 412, 453, 1110
BseSI GKGCMC 2 cut(s) 32, 1087
BseXI GCAGC 1 cut(s) 430
BseYI CCCAGC 1 cut(s) 1076
BsgI GTGCAG 2 cut(s) 429, 553
Bsh1236I CGCG 1 cut(s) 883
BshFI GGCC 1 cut(s) 972
BshVI ATCGAT 1 cut(s) 798
BsiHKAI GWGCWC 1 cut(s) 843
BsiHKCI CYCGRG 1 cut(s) 774
BsiSI CCGG 1 cut(s) 376
BslFI GGGAC 2 cut(s) 540, 802
BslI CCNNNNNNNGG 3 cut(s) 156, 564, 701
BsmAI GTCTC 4 cut(s) 101, 335, 832, 966
BsmBI CGTCTC 1 cut(s) 335
BsmFI GGGAC 2 cut(s) 540, 802
BsmI GAATGC 1 cut(s) 134
BsnI GGCC 1 cut(s) 972
BsoBI CYCGRG 1 cut(s) 774
Bsp1286I GDGCHC 3 cut(s) 32, 843, 1087
Bsp143I GATC 2 cut(s) 235, 547
Bsp1720I GCTNAGC 2 cut(s) 577, 837
BspACI CCGC 4 cut(s) 479, 857, 883, 902
BspANI GGCC 1 cut(s) 972
BspCNI CTCAG 3 cut(s) 418, 590, 829
BspDI ATCGAT 1 cut(s) 798
BspFNI CGCG 1 cut(s) 883
BspLI GGNNCC 2 cut(s) 39, 549
BspMAI CTGCAG 1 cut(s) 930
BspPI GGATC 2 cut(s) 542, 555
BspQI GCTCTTC 2 cut(s) 505, 954
BsrDI GCAATG 1 cut(s) 790
BsrI ACTGG 2 cut(s) 325, 656
BssECI CCNNGG 4 cut(s) 66, 455, 701, 820
BssMI GATC 2 cut(s) 235, 547
BssNI GRCGYC 1 cut(s) 82
BssT1I CCWWGG 2 cut(s) 66, 455
Bst2UI CCWGG 4 cut(s) 87, 702, 821, 1020
Bst4CI ACNGT 3 cut(s) 531, 696, 999
Bst6I CTCTTC 4 cut(s) 505, 525, 835, 954
BstACI GRCGYC 1 cut(s) 82
BstC8I GCNNGC 1 cut(s) 900
BstDEI CTNAG 5 cut(s) 33, 426, 577, 837, 1072
BstEII GGTNACC 1 cut(s) 398
BstF5I GGATG 5 cut(s) 62, 277, 448, 693, 730
BstFNI CGCG 1 cut(s) 883
BstHHI GCGC 1 cut(s) 883
BstKTI GATC 2 cut(s) 238, 550
BstMAI GTCTC 4 cut(s) 101, 335, 832, 966
BstMBI GATC 2 cut(s) 235, 547
BstMWI GCNNNNNNNGC 2 cut(s) 476, 1082
BstNI CCWGG 4 cut(s) 87, 702, 821, 1020
BstNSI RCATGY 1 cut(s) 795
BstPI GGTNACC 1 cut(s) 398
BstSCI CCNGG 4 cut(s) 85, 700, 819, 1018
BstSFI CTRYAG 1 cut(s) 926
BstSLI GKGCMC 2 cut(s) 32, 1087
BstUI CGCG 1 cut(s) 883
BstV1I GCAGC 1 cut(s) 430
BstV2I GAAGAC 2 cut(s) 4, 64
BstX2I RGATCY 1 cut(s) 547
BstXI CCANNNNNNTGG 1 cut(s) 320
BstYI RGATCY 1 cut(s) 547
Bsu15I ATCGAT 1 cut(s) 798
BsuI GTATCC 1 cut(s) 345
BsuRI GGCC 1 cut(s) 972
BsuTUI ATCGAT 1 cut(s) 798
BtsCI GGATG 5 cut(s) 62, 277, 448, 693, 730
BtsIMutI CAGTG 1 cut(s) 318
Cac8I GCNNGC 1 cut(s) 900
CaiI CAGNNNCTG 1 cut(s) 529
CfoI GCGC 1 cut(s) 883
Cfr13I GGNCC 1 cut(s) 37
ClaI ATCGAT 1 cut(s) 798
CseI GACGC 1 cut(s) 71
CsiI ACCWGGT 1 cut(s) 1018
Csp6I GTAC 1 cut(s) 1158
CviAII CATG 6 cut(s) 167, 175, 356, 363, 792, 988
CviQI GTAC 1 cut(s) 1158
DdeI CTNAG 5 cut(s) 33, 426, 577, 837, 1072
DpnI GATC 2 cut(s) 237, 549
DpnII GATC 2 cut(s) 235, 547
DraI TTTAAA 1 cut(s) 1033
DraIII CACNNNGTG 1 cut(s) 268
Eam1104I CTCTTC 4 cut(s) 505, 525, 835, 954
EarI CTCTTC 4 cut(s) 505, 525, 835, 954
Eco130I CCWWGG 2 cut(s) 66, 455
Eco47I GGWCC 1 cut(s) 37
Eco57I CTGAAG 3 cut(s) 318, 506, 816
Eco88I CYCGRG 1 cut(s) 774
Eco91I GGTNACC 1 cut(s) 398
EcoO109I RGGNCCY 1 cut(s) 37
EcoO65I GGTNACC 1 cut(s) 398
EcoRII CCWGG 4 cut(s) 85, 700, 819, 1018
EcoT14I CCWWGG 2 cut(s) 66, 455
ErhI CCWWGG 2 cut(s) 66, 455
Esp3I CGTCTC 1 cut(s) 335
FaeI CATG 6 cut(s) 170, 178, 359, 366, 795, 991
FalI AAGNNNNNCTT 2 cut(s) 468, 500
FaqI GGGAC 2 cut(s) 540, 802
FatI CATG 6 cut(s) 166, 174, 355, 362, 791, 987
FauI CCCGC 2 cut(s) 850, 876
Fnu4HI GCNGC 2 cut(s) 444, 480
FokI GGATG 5 cut(s) 49, 284, 435, 700, 737
Fsp4HI GCNGC 2 cut(s) 444, 480
FspBI CTAG 1 cut(s) 404
GlaI GCGC 1 cut(s) 882
GluI GCNGC 2 cut(s) 444, 480
GsaI CCCAGC 1 cut(s) 1080
GsuI CTGGAG 3 cut(s) 108, 639, 803
HaeIII GGCC 1 cut(s) 972
HapII CCGG 1 cut(s) 376
HgaI GACGC 1 cut(s) 71
HhaI GCGC 1 cut(s) 883
Hin1I GRCGYC 1 cut(s) 82
Hin1II CATG 6 cut(s) 170, 178, 359, 366, 795, 991
Hin6I GCGC 1 cut(s) 881
HinP1I GCGC 1 cut(s) 881
HindIII AAGCTT 3 cut(s) 598, 609, 867
HinfI GANTC 3 cut(s) 90, 412, 959
HpaII CCGG 1 cut(s) 376
HphI GGTGA 3 cut(s) 236, 392, 721
Hpy166II GTNNAC 2 cut(s) 206, 497
Hpy188I TCNGA 3 cut(s) 298, 427, 663
Hpy188III TCNNGA 6 cut(s) 16, 125, 338, 485, 541, 776
Hpy8I GTNNAC 2 cut(s) 206, 497
HpyAV CCTTC 5 cut(s) 293, 481, 538, 983, 1012
HpyCH4III ACNGT 3 cut(s) 531, 696, 999
HpyCH4IV ACGT 2 cut(s) 352, 843
HpyCH4V TGCA 7 cut(s) 178, 275, 446, 534, 616, 898, 928
HpyF10VI GCNNNNNNNGC 2 cut(s) 476, 1082
HpyF3I CTNAG 5 cut(s) 33, 426, 577, 837, 1072
HpySE526I ACGT 2 cut(s) 352, 843
Hsp92I GRCGYC 1 cut(s) 82
Hsp92II CATG 6 cut(s) 170, 178, 359, 366, 795, 991
HspAI GCGC 1 cut(s) 881
Kzo9I GATC 2 cut(s) 235, 547
LguI GCTCTTC 2 cut(s) 505, 954
LmnI GCTCC 2 cut(s) 346, 440
Lsp1109I GCAGC 1 cut(s) 430
LweI GCATC 5 cut(s) 201, 262, 299, 457, 524
MabI ACCWGGT 1 cut(s) 1018
MaeI CTAG 1 cut(s) 404
MaeII ACGT 2 cut(s) 352, 843
MaeIII GTNAC 4 cut(s) 48, 188, 358, 398
MalI GATC 2 cut(s) 237, 549
MboI GATC 2 cut(s) 235, 547
MfeI CAATTG 1 cut(s) 570
MflI RGATCY 1 cut(s) 547
MhlI GDGCHC 3 cut(s) 32, 843, 1087
MluCI AATT 5 cut(s) 151, 314, 570, 736, 1013
MlyI GAGTC 2 cut(s) 99, 968
MseI TTAA 3 cut(s) 735, 1032, 1162
MslI CAYNNNNRTG 2 cut(s) 405, 621
MspA1I CMGCKG 1 cut(s) 836
MspI CCGG 1 cut(s) 376
MspR9I CCNGG 4 cut(s) 87, 702, 821, 1020
MunI CAATTG 1 cut(s) 570
Mva1269I GAATGC 1 cut(s) 134
MvaI CCWGG 4 cut(s) 87, 702, 821, 1020
MvnI CGCG 1 cut(s) 883
MwoI GCNNNNNNNGC 2 cut(s) 476, 1082
NdeII GATC 2 cut(s) 235, 547
NlaIII CATG 6 cut(s) 170, 178, 359, 366, 795, 991
NlaIV GGNNCC 2 cut(s) 39, 549
NmuCI GTSAC 3 cut(s) 188, 358, 398
NspI RCATGY 1 cut(s) 795
PaeR7I CTCGAG 1 cut(s) 774
PciI ACATGT 1 cut(s) 791
PciSI GCTCTTC 2 cut(s) 505, 954
PctI GAATGC 1 cut(s) 134
PfeI GAWTC 1 cut(s) 412
PfoI TCCNGGA 1 cut(s) 85
PkrI GCNGC 2 cut(s) 445, 481
PleI GAGTC 2 cut(s) 98, 967
PpsI GAGTC 2 cut(s) 98, 967
PpuMI RGGWCCY 1 cut(s) 37
PscI ACATGT 1 cut(s) 791
Psp5II RGGWCCY 1 cut(s) 37
Psp6I CCWGG 4 cut(s) 85, 700, 819, 1018
PspEI GGTNACC 1 cut(s) 398
PspFI CCCAGC 1 cut(s) 1076
PspGI CCWGG 4 cut(s) 85, 700, 819, 1018
PspN4I GGNNCC 2 cut(s) 39, 549
PspPI GGNCC 1 cut(s) 37
PspPPI RGGWCCY 1 cut(s) 37
PstI CTGCAG 1 cut(s) 930
PstNI CAGNNNCTG 1 cut(s) 529
PsuI RGATCY 1 cut(s) 547
PvuII CAGCTG 1 cut(s) 836
RsaI GTAC 1 cut(s) 1159
RsaNI GTAC 1 cut(s) 1158
RseI CAYNNNNRTG 2 cut(s) 405, 621
SapI GCTCTTC 2 cut(s) 505, 954
SaqAI TTAA 3 cut(s) 735, 1032, 1162
SatI GCNGC 2 cut(s) 444, 480
Sau3AI GATC 2 cut(s) 235, 547
Sau96I GGNCC 1 cut(s) 37
SchI GAGTC 2 cut(s) 99, 968
ScrFI CCNGG 4 cut(s) 87, 702, 821, 1020
SduI GDGCHC 3 cut(s) 32, 843, 1087
SexAI ACCWGGT 1 cut(s) 1018
SfaNI GCATC 5 cut(s) 201, 262, 299, 457, 524
SfcI CTRYAG 1 cut(s) 926
Sfr274I CTCGAG 1 cut(s) 774
SinI GGWCC 1 cut(s) 37
SlaI CTCGAG 1 cut(s) 774
SmiMI CAYNNNNRTG 2 cut(s) 405, 621
SmlI CTYRAG 1 cut(s) 774
SmoI CTYRAG 1 cut(s) 774
Sse9I AATT 5 cut(s) 151, 314, 570, 736, 1013
SsiI CCGC 4 cut(s) 479, 857, 883, 902
SspI AATATT 1 cut(s) 22
SspMI CTAG 1 cut(s) 404
StyD4I CCNGG 4 cut(s) 85, 700, 819, 1018
StyI CCWWGG 2 cut(s) 66, 455
TaaI ACNGT 3 cut(s) 531, 696, 999
TaiI ACGT 2 cut(s) 355, 846
TaqI TCGA 6 cut(s) 145, 307, 540, 775, 798, 941
TaqII GACCGA 1 cut(s) 386
TasI AATT 5 cut(s) 151, 314, 570, 736, 1013
TatI WGTACW 1 cut(s) 1157
TauI GCSGC 1 cut(s) 482
TfiI GAWTC 1 cut(s) 412
Tru1I TTAA 3 cut(s) 735, 1032, 1162
Tru9I TTAA 3 cut(s) 735, 1032, 1162
TscAI CASTG 1 cut(s) 325
TseFI GTSAC 3 cut(s) 188, 358, 398
TseI GCWGC 1 cut(s) 443
Tsp45I GTSAC 3 cut(s) 188, 358, 398
TspDTI ATGAA 2 cut(s) 230, 704
TspGWI ACGGA 1 cut(s) 359
TspRI CASTG 1 cut(s) 325
VpaK11BI GGWCC 1 cut(s) 37
XceI RCATGY 1 cut(s) 795
XcmI CCANNNNNNNNNTGG 1 cut(s) 83
XhoI CTCGAG 1 cut(s) 774
XspI CTAG 1 cut(s) 404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.