Rmu_sc0026800.1_g000001

ribonuclease H protein At1g65750-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0026800.1
OG0005205
Physical Location & Seq
Forward (+)
98 .. 688
591 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0026800.1_g000001.1.cds

Sequence Viewer

Length: 591 bp
atggatatagttttctggaatatcagaggcactaaggaccctcgtaagttacaggcgttgtcatccttggtcttccaaaggtgtcctggagtcctatttctgttggagacgaagtgtagtcaagaacgcttcaactcctttcgagctaatctaaggtttcaacatggagtgatgcaggagtgtagcgataatggagggggtggtctgatgctgttatggggtgaggacattgatattcatcttcgctcctattccccgcactttgtggatgcagatgtggtgttgccggcgtctaaagggggtcgttgccggatcactgatttttacgggcatccagagacaacgcaacgtcatgtcacttgggatttactcaggcaacttgccatgcttcccgaggcagataagtggttagtatttgttgacctaaacgaaattctttcctcctttgagaaatctggaggagccgcacgtcctctatggcagatcaatgctttccaagcggctcttgacgaagggttttcgactttaataaggagaatgccactactagtaaagagagctatatacactgatttcaatctgtgtgtataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

196

Amino Acids

22.38

Weight (kDa)

6.95

Isoelectric Point (pI)

43.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 257, 465, 500
AclWI GGATC 1 cut(s) 320
AcsI RAATTY 1 cut(s) 432
AcyI GRCGYC 1 cut(s) 290
AdeI CACNNNGTG 1 cut(s) 265
AgsI TTSAA 3 cut(s) 133, 161, 577
AhlI ACTAGT 1 cut(s) 547
AjiI CACGTC 1 cut(s) 470
AjnI CCWGG 1 cut(s) 85
AluBI AGCT 2 cut(s) 146, 560
AluI AGCT 2 cut(s) 146, 560
Alw26I GTCTC 2 cut(s) 101, 332
AlwI GGATC 1 cut(s) 320
Ama87I CYCGRG 1 cut(s) 392
ApoI RAATTY 1 cut(s) 432
AspS9I GGNCC 1 cut(s) 37
AsuHPI GGTGA 1 cut(s) 233
AvaI CYCGRG 1 cut(s) 392
AvaII GGWCC 1 cut(s) 37
BbsI GAAGAC 1 cut(s) 64
BciT130I CCWGG 1 cut(s) 87
BcoDI GTCTC 2 cut(s) 101, 332
BcuI ACTAGT 1 cut(s) 547
BfaI CTAG 1 cut(s) 548
BisI GCNGC 2 cut(s) 465, 501
BlsI GCNGC 2 cut(s) 466, 502
Bme1390I CCNGG 1 cut(s) 87
Bme18I GGWCC 1 cut(s) 37
BmeT110I CYCGRG 1 cut(s) 392
BmgBI CACGTC 1 cut(s) 470
BmgT120I GGNCC 1 cut(s) 37
BmiI GGNNCC 2 cut(s) 39, 463
BmrFI CCNGG 1 cut(s) 87
BmsI GCATC 4 cut(s) 162, 198, 259, 340
BpiI GAAGAC 1 cut(s) 64
BpmI CTGGAG 2 cut(s) 108, 477
BsaBI GATNNNNATC 2 cut(s) 237, 576
BsaHI GRCGYC 1 cut(s) 290
BsaJI CCNNGG 2 cut(s) 66, 393
BsaXI ACNNNNNCTCC 2 cut(s) 98, 128
Bse118I RCCGGY 1 cut(s) 286
Bse8I GATNNNNATC 2 cut(s) 237, 576
BseBI CCWGG 1 cut(s) 87
BseDI CCNNGG 2 cut(s) 66, 393
BseGI GGATG 3 cut(s) 62, 274, 331
BseJI GATNNNNATC 2 cut(s) 237, 576
BseMII CTCAG 1 cut(s) 385
BseRI GAGGAG 1 cut(s) 474
BsiHKCI CYCGRG 1 cut(s) 392
BsiSI CCGG 2 cut(s) 287, 310
BsmAI GTCTC 2 cut(s) 101, 332
BsmBI CGTCTC 1 cut(s) 101
BsmI GAATGC 1 cut(s) 543
BsoBI CYCGRG 1 cut(s) 392
Bsp143I GATC 2 cut(s) 312, 483
BspACI CCGC 3 cut(s) 257, 465, 500
BspCNI CTCAG 1 cut(s) 384
BspLI GGNNCC 2 cut(s) 39, 463
BspPI GGATC 1 cut(s) 320
BsrFI RCCGGY 1 cut(s) 286
BssAI RCCGGY 1 cut(s) 286
BssECI CCNNGG 2 cut(s) 66, 393
BssMI GATC 2 cut(s) 312, 483
BssNI GRCGYC 1 cut(s) 290
BssT1I CCWWGG 1 cut(s) 66
Bst2UI CCWGG 1 cut(s) 87
BstACI GRCGYC 1 cut(s) 290
BstC8I GCNNGC 1 cut(s) 288
BstDEI CTNAG 3 cut(s) 33, 152, 371
BstF5I GGATG 3 cut(s) 62, 274, 331
BstKTI GATC 2 cut(s) 315, 486
BstMAI GTCTC 2 cut(s) 101, 332
BstMBI GATC 2 cut(s) 312, 483
BstMWI GCNNNNNNNGC 1 cut(s) 497
BstNI CCWGG 1 cut(s) 87
BstSCI CCNGG 1 cut(s) 85
BstV2I GAAGAC 1 cut(s) 64
BtrI CACGTC 1 cut(s) 470
BtsCI GGATG 3 cut(s) 62, 274, 331
BtsIMutI CAGTG 2 cut(s) 315, 567
Cac8I GCNNGC 1 cut(s) 288
Cfr10I RCCGGY 1 cut(s) 286
Cfr13I GGNCC 1 cut(s) 37
CseI GACGC 1 cut(s) 279
CviAII CATG 3 cut(s) 164, 353, 385
CviJI RGCY 4 cut(s) 146, 464, 503, 560
CviKI_1 RGCY 4 cut(s) 146, 464, 503, 560
DdeI CTNAG 3 cut(s) 33, 152, 371
DpnI GATC 2 cut(s) 314, 485
DpnII GATC 2 cut(s) 312, 483
DraIII CACNNNGTG 1 cut(s) 265
Eco130I CCWWGG 1 cut(s) 66
Eco47I GGWCC 1 cut(s) 37
Eco88I CYCGRG 1 cut(s) 392
EcoO109I RGGNCCY 1 cut(s) 37
EcoRII CCWGG 1 cut(s) 85
EcoT14I CCWWGG 1 cut(s) 66
ErhI CCWWGG 1 cut(s) 66
Esp3I CGTCTC 1 cut(s) 101
FaeI CATG 3 cut(s) 167, 356, 388
FaiI YATR 9 cut(s) 8, 165, 217, 354, 386, 478, 563, 565, 589
FalI AAGNNNNNCTT 2 cut(s) 489, 521
FatI CATG 3 cut(s) 163, 352, 384
FauI CCCGC 1 cut(s) 264
Fnu4HI GCNGC 2 cut(s) 465, 501
FokI GGATG 3 cut(s) 49, 281, 318
Fsp4HI GCNGC 2 cut(s) 465, 501
FspBI CTAG 1 cut(s) 548
GluI GCNGC 2 cut(s) 465, 501
GsuI CTGGAG 2 cut(s) 108, 477
HapII CCGG 2 cut(s) 287, 310
HgaI GACGC 1 cut(s) 279
Hin1I GRCGYC 1 cut(s) 290
Hin1II CATG 3 cut(s) 167, 356, 388
HincII GTYRAC 1 cut(s) 421
HindII GTYRAC 1 cut(s) 421
HinfI GANTC 1 cut(s) 90
HpaII CCGG 2 cut(s) 287, 310
HphI GGTGA 1 cut(s) 233
Hpy166II GTNNAC 1 cut(s) 421
Hpy188I TCNGA 2 cut(s) 26, 207
Hpy188III TCNNGA 6 cut(s) 16, 122, 335, 392, 456, 506
Hpy8I GTNNAC 1 cut(s) 421
HpyAV CCTTC 1 cut(s) 506
HpyCH4IV ACGT 2 cut(s) 349, 469
HpyCH4V TGCA 2 cut(s) 175, 272
HpyF10VI GCNNNNNNNGC 1 cut(s) 497
HpyF3I CTNAG 3 cut(s) 33, 152, 371
HpySE526I ACGT 2 cut(s) 349, 469
Hsp92I GRCGYC 1 cut(s) 290
Hsp92II CATG 3 cut(s) 167, 356, 388
KroI GCCGGC 1 cut(s) 286
KroNI GCCGGC 1 cut(s) 288
Kzo9I GATC 2 cut(s) 312, 483
LmnI GCTCC 2 cut(s) 251, 461
LpnPI CCDG 9 cut(s) 38, 72, 99, 161, 300, 323, 348, 358, 441
LweI GCATC 4 cut(s) 162, 198, 259, 340
MaeI CTAG 1 cut(s) 548
MaeII ACGT 2 cut(s) 349, 469
MaeIII GTNAC 2 cut(s) 48, 355
MalI GATC 2 cut(s) 314, 485
MboI GATC 2 cut(s) 312, 483
MboII GAAGA 2 cut(s) 64, 233
MluCI AATT 1 cut(s) 432
MlyI GAGTC 1 cut(s) 99
MmeI TCCRAC 1 cut(s) 84
MnlI CCTC 8 cut(s) 20, 51, 188, 217, 388, 451, 452, 483
MroNI GCCGGC 1 cut(s) 286
MseI TTAA 1 cut(s) 527
MspI CCGG 2 cut(s) 287, 310
MspR9I CCNGG 1 cut(s) 87
Mva1269I GAATGC 1 cut(s) 543
MvaI CCWGG 1 cut(s) 87
MwoI GCNNNNNNNGC 1 cut(s) 497
NaeI GCCGGC 1 cut(s) 288
NdeII GATC 2 cut(s) 312, 483
NgoMIV GCCGGC 1 cut(s) 286
NlaIII CATG 3 cut(s) 167, 356, 388
NlaIV GGNNCC 2 cut(s) 39, 463
NmuCI GTSAC 1 cut(s) 355
PctI GAATGC 1 cut(s) 543
PdiI GCCGGC 1 cut(s) 288
PfoI TCCNGGA 1 cut(s) 85
PkrI GCNGC 2 cut(s) 466, 502
PleI GAGTC 1 cut(s) 98
PpsI GAGTC 1 cut(s) 98
PpuMI RGGWCCY 1 cut(s) 37
Psp5II RGGWCCY 1 cut(s) 37
Psp6I CCWGG 1 cut(s) 85
PspGI CCWGG 1 cut(s) 85
PspN4I GGNNCC 2 cut(s) 39, 463
PspPI GGNCC 1 cut(s) 37
PspPPI RGGWCCY 1 cut(s) 37
SaqAI TTAA 1 cut(s) 527
SatI GCNGC 2 cut(s) 465, 501
Sau3AI GATC 2 cut(s) 312, 483
Sau96I GGNCC 1 cut(s) 37
SchI GAGTC 1 cut(s) 99
ScrFI CCNGG 1 cut(s) 87
SetI ASST 7 cut(s) 83, 148, 158, 352, 426, 472, 562
SfaNI GCATC 4 cut(s) 162, 198, 259, 340
SinI GGWCC 1 cut(s) 37
SpeI ACTAGT 1 cut(s) 547
Sse9I AATT 1 cut(s) 432
SsiI CCGC 3 cut(s) 257, 465, 500
SspMI CTAG 1 cut(s) 548
StyD4I CCNGG 1 cut(s) 85
StyI CCWWGG 1 cut(s) 66
TaiI ACGT 2 cut(s) 352, 472
TaqI TCGA 2 cut(s) 142, 521
TasI AATT 1 cut(s) 432
TauI GCSGC 2 cut(s) 467, 503
Tru1I TTAA 1 cut(s) 527
Tru9I TTAA 1 cut(s) 527
TscAI CASTG 2 cut(s) 322, 574
TseFI GTSAC 1 cut(s) 355
Tsp45I GTSAC 1 cut(s) 355
TspDTI ATGAA 1 cut(s) 227
TspRI CASTG 2 cut(s) 322, 574
VpaK11BI GGWCC 1 cut(s) 37
XapI RAATTY 1 cut(s) 432
XcmI CCANNNNNNNNNTGG 1 cut(s) 83
XspI CTAG 1 cut(s) 548
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.