Rh4CG173800

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0005205
Physical Location & Seq
Forward (+)
37979886 .. 37998240
18355 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG173800.1

Sequence Viewer

Length: 993 bp
ATGGAGATTCTATCATGGAATATCAGGGGTACCAAAGACCCTAAGAAATTCAGGGCTTTGGCGTCGATTATTCAGCAGAGACGTCCTAGTATTGTGTTCTTGATCGAGACAAAGTGTAGAGACAAGCCAGCATTCAAACGGTTCCGCAACGATTTGGGTTTCCGTCATGGCATAATGCAAGAGTGTTCTAATACGGGTGGAGGAGGTTTGATGCTATTGTGGGTGGATGAAATTGATCTTCATCTCCGGTCGTACTCACCCCATCACATTGATGTAGATGTTGTGCTACCGGAATCTGAGGGAGGGAGATGGCGACTCACAGATTTAGGGTTTGAGGGCAGTCTATTCACATGGCATAATTCAGAGACTCAATGTAGATTAGACCGTGCAATTGCCACGCAGGGTTGGTGTCAAGTGTATGGACTGAATAGGGTGCTCTACCTTCCACCCTCTTGTTCTGATCATGTCCCGATTCTTGTAAAAGCACATATTCAGGCTCCAATTCAGTTTCCATCACGCAGAACCAGATTTCGGTTTGAAGAGATGTGGACGAAGGATGATTCTTGTGAAGATGTGATCCGGAAGAAGTGGCAGACGGATAGGGATGGTTTCGAGCAGTTTCGGTTGCAGGGAAAACTTTATAGCACGAGGTTGGGTTTACTCAATTGGAAGAGGCAGGTTGAAGATCTTGCTGACTTTTCCTTCCAGACAACGCCTATGTATATAGATGTGGATGATGGGAGAACTAAGGCCACGAATATAGGACTGCAACAGGGCTATTGTCTTGTGCCAAGTGCCAAGAGATTTTATTCTCCTATATTCTTTCCTGACGAGGAATCTATCAAAGAAAGTGATGAATCCGTAACACAATCAGGGGTGCCACGGCTTGGCTCAATAGTCATCCCACAAGCAGAAACTGTAGATTCATTTCACCTTCTCCCTCCCACAGAAAACTATGTTCCTCTATGCAAGGATGAGTGGAGATTTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

330

Amino Acids

38.35

Weight (kDa)

6.72

Isoelectric Point (pI)

56.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 4 - 86 2.9e-07 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 85
Acc36I ACCTGC 1 cut(s) 667
Acc65I GGTACC 1 cut(s) 29
AccB1I GGYRCC 2 cut(s) 29, 877
AccB7I CCANNNNNTGG 1 cut(s) 887
AccIII TCCGGA 1 cut(s) 579
AciI CCGC 1 cut(s) 145
AclWI GGATC 1 cut(s) 571
AcsI RAATTY 1 cut(s) 47
AcyI GRCGYC 2 cut(s) 62, 82
AfaI GTAC 2 cut(s) 31, 254
AfiI CCNNNNNNNGG 2 cut(s) 531, 887
AgsI TTSAA 3 cut(s) 136, 539, 683
Alw21I GWGCWC 1 cut(s) 438
Alw26I GTCTC 4 cut(s) 73, 101, 114, 359
AlwI GGATC 1 cut(s) 571
AlwNI CAGNNNCTG 1 cut(s) 917
Aor13HI TCCGGA 1 cut(s) 579
AoxI GGCC 1 cut(s) 750
ApoI RAATTY 1 cut(s) 47
Asp718I GGTACC 1 cut(s) 29
AsuHPI GGTGA 2 cut(s) 249, 923
BanI GGYRCC 2 cut(s) 29, 877
BauI CACGAG 1 cut(s) 646
Bbv12I GWGCWC 1 cut(s) 438
BccI CCATC 5 cut(s) 270, 303, 520, 599, 731
BceAI ACGGC 1 cut(s) 899
BclI TGATCA 1 cut(s) 460
BcoDI GTCTC 4 cut(s) 73, 101, 114, 359
BfaI CTAG 1 cut(s) 87
BfmI CTRYAG 1 cut(s) 918
BfuAI ACCTGC 1 cut(s) 667
BglII AGATCT 1 cut(s) 685
BmiI GGNNCC 4 cut(s) 31, 143, 498, 879
BmsI GCATC 1 cut(s) 201
BsaBI GATNNNNATC 1 cut(s) 240
BsaHI GRCGYC 2 cut(s) 62, 82
BsaJI CCNNGG 1 cut(s) 881
BsaWI WCCGGW 3 cut(s) 246, 289, 579
Bsc4I CCNNNNNNNGG 2 cut(s) 531, 887
Bse8I GATNNNNATC 1 cut(s) 240
BseAI TCCGGA 1 cut(s) 579
BseDI CCNNGG 1 cut(s) 881
BseGI GGATG 6 cut(s) 232, 562, 610, 739, 900, 979
BseJI GATNNNNATC 1 cut(s) 240
BseLI CCNNNNNNNGG 2 cut(s) 531, 887
BseMII CTCAG 1 cut(s) 288
BseRI GAGGAG 1 cut(s) 216
Bsh1285I CGRYCG 1 cut(s) 251
BshFI GGCC 1 cut(s) 752
BshNI GGYRCC 2 cut(s) 29, 877
BsiEI CGRYCG 1 cut(s) 251
BsiHKAI GWGCWC 1 cut(s) 438
BsiSI CCGG 3 cut(s) 247, 290, 580
BslFI GGGAC 1 cut(s) 452
BslI CCNNNNNNNGG 2 cut(s) 531, 887
BsmAI GTCTC 4 cut(s) 73, 101, 114, 359
BsmBI CGTCTC 1 cut(s) 73
BsmFI GGGAC 1 cut(s) 452
BsmI GAATGC 1 cut(s) 131
BsnI GGCC 1 cut(s) 752
Bsp1286I GDGCHC 1 cut(s) 438
Bsp13I TCCGGA 1 cut(s) 579
Bsp143I GATC 5 cut(s) 102, 235, 460, 576, 685
BspACI CCGC 1 cut(s) 145
BspANI GGCC 1 cut(s) 752
BspCNI CTCAG 1 cut(s) 289
BspEI TCCGGA 1 cut(s) 579
BspLI GGNNCC 4 cut(s) 31, 143, 498, 879
BspMI ACCTGC 1 cut(s) 667
BspPI GGATC 1 cut(s) 571
BspT107I GGYRCC 2 cut(s) 29, 877
BssECI CCNNGG 1 cut(s) 881
BssMI GATC 5 cut(s) 102, 235, 460, 576, 685
BssNI GRCGYC 2 cut(s) 62, 82
BssSI CACGAG 1 cut(s) 646
Bst2BI CACGAG 1 cut(s) 646
Bst4CI ACNGT 3 cut(s) 141, 386, 919
Bst6I CTCTTC 2 cut(s) 534, 665
BstACI GRCGYC 2 cut(s) 62, 82
BstC8I GCNNGC 1 cut(s) 129
BstDEI CTNAG 3 cut(s) 42, 297, 747
BstDSI CCRYGG 1 cut(s) 881
BstF5I GGATG 6 cut(s) 232, 562, 610, 739, 900, 979
BstKTI GATC 5 cut(s) 105, 238, 463, 579, 688
BstMAI GTCTC 4 cut(s) 73, 101, 114, 359
BstMBI GATC 5 cut(s) 102, 235, 460, 576, 685
BstMCI CGRYCG 1 cut(s) 251
BstSFI CTRYAG 1 cut(s) 918
BstX2I RGATCY 1 cut(s) 685
BstYI RGATCY 1 cut(s) 685
BsuRI GGCC 1 cut(s) 752
BtgI CCRYGG 1 cut(s) 881
BtsCI GGATG 6 cut(s) 232, 562, 610, 739, 900, 979
BveI ACCTGC 1 cut(s) 667
Cac8I GCNNGC 1 cut(s) 129
CaiI CAGNNNCTG 1 cut(s) 917
CseI GACGC 1 cut(s) 51
Csp6I GTAC 2 cut(s) 30, 253
CviAII CATG 4 cut(s) 15, 167, 351, 464
CviJI RGCY 7 cut(s) 56, 127, 497, 752, 777, 886, 891
CviKI_1 RGCY 7 cut(s) 56, 127, 497, 752, 777, 886, 891
CviQI GTAC 2 cut(s) 30, 253
DdeI CTNAG 3 cut(s) 42, 297, 747
DpnI GATC 5 cut(s) 104, 237, 462, 578, 687
DpnII GATC 5 cut(s) 102, 235, 460, 576, 685
Eam1104I CTCTTC 2 cut(s) 534, 665
EarI CTCTTC 2 cut(s) 534, 665
Esp3I CGTCTC 1 cut(s) 73
FaeI CATG 4 cut(s) 18, 170, 354, 467
FaqI GGGAC 1 cut(s) 452
FatI CATG 4 cut(s) 14, 166, 350, 463
FbaI TGATCA 1 cut(s) 460
FokI GGATG 6 cut(s) 239, 569, 617, 746, 887, 986
FspBI CTAG 1 cut(s) 87
HaeIII GGCC 1 cut(s) 752
HapII CCGG 3 cut(s) 247, 290, 580
HgaI GACGC 1 cut(s) 51
Hin1I GRCGYC 2 cut(s) 62, 82
Hin1II CATG 4 cut(s) 18, 170, 354, 467
HinfI GANTC 9 cut(s) 7, 293, 315, 367, 472, 560, 836, 857, 923
HpaII CCGG 3 cut(s) 247, 290, 580
HphI GGTGA 2 cut(s) 249, 923
Hpy166II GTNNAC 2 cut(s) 549, 659
Hpy188I TCNGA 3 cut(s) 298, 364, 460
Hpy188III TCNNGA 6 cut(s) 100, 106, 469, 580, 706, 827
Hpy8I GTNNAC 2 cut(s) 549, 659
Hpy99I CGWCG 1 cut(s) 67
HpyAV CCTTC 4 cut(s) 452, 547, 712, 944
HpyCH4III ACNGT 3 cut(s) 141, 386, 919
HpyCH4IV ACGT 1 cut(s) 82
HpyCH4V TGCA 5 cut(s) 178, 389, 628, 769, 969
HpyF3I CTNAG 3 cut(s) 42, 297, 747
HpySE526I ACGT 1 cut(s) 82
Hsp92I GRCGYC 2 cut(s) 62, 82
Hsp92II CATG 4 cut(s) 18, 170, 354, 467
Kpn2I TCCGGA 1 cut(s) 579
KpnI GGTACC 1 cut(s) 33
Ksp22I TGATCA 1 cut(s) 460
Kzo9I GATC 5 cut(s) 102, 235, 460, 576, 685
LmnI GCTCC 1 cut(s) 502
LweI GCATC 1 cut(s) 201
MaeI CTAG 1 cut(s) 87
MaeII ACGT 1 cut(s) 82
MaeIII GTNAC 1 cut(s) 862
MalI GATC 5 cut(s) 104, 237, 462, 578, 687
MboI GATC 5 cut(s) 102, 235, 460, 576, 685
MboII GAAGA 6 cut(s) 230, 551, 581, 595, 682, 695
MfeI CAATTG 2 cut(s) 390, 664
MflI RGATCY 1 cut(s) 685
MhlI GDGCHC 1 cut(s) 438
MluCI AATT 6 cut(s) 47, 231, 358, 390, 501, 664
MlyI GAGTC 2 cut(s) 309, 361
MroI TCCGGA 1 cut(s) 579
MslI CAYNNNNRTG 1 cut(s) 270
MspI CCGG 3 cut(s) 247, 290, 580
MunI CAATTG 2 cut(s) 390, 664
Mva1269I GAATGC 1 cut(s) 131
NdeII GATC 5 cut(s) 102, 235, 460, 576, 685
NlaIII CATG 4 cut(s) 18, 170, 354, 467
NlaIV GGNNCC 4 cut(s) 31, 143, 498, 879
PctI GAATGC 1 cut(s) 131
PfeI GAWTC 7 cut(s) 7, 293, 472, 560, 836, 857, 923
PflMI CCANNNNNTGG 1 cut(s) 887
PleI GAGTC 2 cut(s) 309, 361
PpsI GAGTC 2 cut(s) 309, 361
PspN4I GGNNCC 4 cut(s) 31, 143, 498, 879
PstNI CAGNNNCTG 1 cut(s) 917
PsuI RGATCY 1 cut(s) 685
RsaI GTAC 2 cut(s) 31, 254
RsaNI GTAC 2 cut(s) 30, 253
RseI CAYNNNNRTG 1 cut(s) 270
Sau3AI GATC 5 cut(s) 102, 235, 460, 576, 685
SchI GAGTC 2 cut(s) 309, 361
SduI GDGCHC 1 cut(s) 438
SetI ASST 6 cut(s) 85, 208, 444, 653, 681, 936
SfaNI GCATC 1 cut(s) 201
SfcI CTRYAG 1 cut(s) 918
SmiMI CAYNNNNRTG 1 cut(s) 270
Sse9I AATT 6 cut(s) 47, 231, 358, 390, 501, 664
SsiI CCGC 1 cut(s) 145
SspMI CTAG 1 cut(s) 87
TaaI ACNGT 3 cut(s) 141, 386, 919
TaiI ACGT 1 cut(s) 85
TaqI TCGA 3 cut(s) 65, 105, 612
TasI AATT 6 cut(s) 47, 231, 358, 390, 501, 664
TfiI GAWTC 7 cut(s) 7, 293, 472, 560, 836, 857, 923
TspDTI ATGAA 4 cut(s) 230, 243, 870, 915
TspGWI ACGGA 3 cut(s) 152, 611, 850
Van91I CCANNNNNTGG 1 cut(s) 887
XapI RAATTY 1 cut(s) 47
XspI CTAG 1 cut(s) 87
ZraI GACGTC 1 cut(s) 83
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.