RLG00000020347

Protein of unknown function, DUF547

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0001176
Physical Location & Seq
Reverse (-)
63937925 .. 63943988
6064 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020347

Sequence Viewer

Length: 2238 bp
ATGGTCAAACATAACAGCACCCTTCAAATTTTACCCAATATGCCCCTCTTCCCTCGCCCTTTTCTCATCTTCCTCGTGCTCTGTCAAAGCGCAGCCGCACCTTTGTCACTTTTTAAGTGTGACCAGCAAGATTTGAATTTTGAGGAGGGAGAATATGGATGTGACTTCATACCCAAAGAAGAAGAAGAAGAAGCCTTGGAAATTTGGGTCTTTTTTGGTTCTAAAAAGCTTTCTTCAAATACCGATTTCAATCAAGGAGATCCAGGATTTGTGAAAGCTTGTTTGTACCTGCGTATAGTTTCTAGTCTTCTAGTACCAGAAGCCAGGCTGTTTCTGCGTTTGGAGGATTTGCTTTATGTGGTAAAAGCCAAACCCCATGATCACAGGATGATGTGCGACTCACCGATTGTGCAAACTAAGTTGAGCCAAAATGATTCCCTCTGTAAATATTCTCATTCTAGCATCTCATGTGATTCTGAATTTCAGTCAGAGGTTGTGAAAGATGACTCGTTTTCAAAGGTGAGTAATCCTGATGAAGGTATTGAACGACTTGGATCATGCAATAAAAGTTCATACCCGTACAAGTTCCAGCTTGAACAGGATGTACAAAGATTGGAACAGCAGCTGCTTGAAGAAATAGAGCTACATTCTATTCTGGAAAATGCAATTGAGAAAAATGCTACTAAATTCTCCAGTCCAGCATGCCTTCCTTACAATGCTCAAGAGCTCCTAAGCAATATTGCTGCCCTGGAGGTTACAGTTTCAGAACTAGAACAGGAGTTGGTTGCTTTGCATTTCCAACTAAGTCAAGAAAGAAATGAAAGAAGGCTGGCAGAATATCGTCTGAGGCATCCATCTCCTCAGTCAATGTCTCCTCGCTCGTCTGGTATTATAAAACTGCTGAGTTCATCTCCTTCGAGGTGTACAGAGCTGTCTAGTCCTGAAATTCATCATTATTCAGGAGATGACTCATGGCAGGAGCCACAAGATCAATCATCAGAAACTAGTGGTGAAACATCTTCCATGCAATCAGTAACTGAGAATGCGGTGGACTCAGTTGCATGTGGCCACGATATGAAAAACTATAGGAAGATGGATCTTGTATCTTTGCAACCTTCTGACTTCAGGAAGCTTCCTAAAGGGATGCCCTCCAAGGGCCTGTGGGATCATCCTAATCAACTGTCAGAGGAGATGGTTAGATGCATGAAAAATATTTTCATATCTTTGGCTGATTCAGCCTTACCGTCAAAATCTTCTTCCCAAGAGAGCCATTTGTCACCACTGTCACCACGTGGACATCTTTCTAATTCCTCATGGTGGTCATCATCTGAACGGTCGATGATTTCATCATGGGTACAGAGCCCACAAGTAGATATACAGAGTAACTCTGAAGTATTAGCTTCAGAAAACGCCTGTGATCCCTATAGAGTTCGAGGGAAACTGAGTTGGGCAGACATTGGGAGCTATGGACTAGCAACTGAAGTTTCTTGGATGTCAGTTGGGAAGAAGCAATTAGAATATGCTGCTGGTGCTCTGAGGAGGTTCAGAGTACTTGTGGAGCAACTGGCAAAAGTAAATCCTATCCATTTGAGCTGCAATGAGAAGCTTGCTTTCTGGATTAACTTATATAATGCACTTATCATGCATGCTTACTTGGCTTACGGAGTCCCTAGAAGTGATTTGAAGCTCTTCTCTTTAATGCAAAAGGCAGCTTACACTGTCGGGGGACATTCTTACAGTGCCACTGCTATTGAGTATGTAATTTTGAAGGCGAAACCTCCACTTCATAGACCACAAATTGCTTTGCTTCTTGCCCTTCACAAGCTTAAGGTCTCAGACGAGCAACGGAAGTCTGTAATCGATACATATGAACCACTTGTAACCTTTGCTTTAAGCTGTGGAATGTACTCCTCACCGGCGGTAAGGATCTACACTGCTAAAAATGTAAGGGAAGAACTTCAGGAAGCACAGCGTGATTTTGTTCGAGCTTCAGTTGGGGTTAGTAACAAGGGGAGGTTGTTGGTGCCAAAATTGTTGCACTGCTTTGCCAAAGGCTCTGTGGATGATTCAAATTTGGCTGTATGGATATCACATTACCTCCCGCCCCACCAAGCTATCTTTGTTGAACAATGCATATCACAAAGGCGACAAAGTCTGCTTGGTTCGCGCAACTGTGGCATTTTGCCATTTGATTCACGCTTCCGATACCTATTTCTGCCCGACAGAATTCCATTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF547 Lzipper-MIP1

Protein Analysis

746

Amino Acids

84.02

Weight (kDa)

6.19

Isoelectric Point (pI)

67.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lzipper-MIP1 PF14389 194 - 271 4.4e-20 Leucine-zipper of ternary complex factor MIP1
DUF547 PF04784 529 - 661 3e-41 Protein of unknown function, DUF547
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 893
Acc36I ACCTGC 1 cut(s) 297
AccB1I GGYRCC 1 cut(s) 2023
AccII CGCG 1 cut(s) 2167
AciI CCGC 4 cut(s) 96, 1046, 1919, 2102
AclWI GGATC 6 cut(s) 254, 562, 1104, 1173, 1412, 1934
AcoI YGGCCR 1 cut(s) 1066
AcsI RAATTY 8 cut(s) 27, 136, 201, 479, 686, 945, 2071, 2226
AcuI CTGAAG 6 cut(s) 1108, 1386, 1410, 1500, 1943, 1974
AcvI CACGTG 1 cut(s) 1292
AdeI CACNNNGTG 2 cut(s) 1292, 1973
AfaI GTAC 8 cut(s) 287, 315, 581, 606, 925, 1356, 1551, 1907
AfiI CCNNNNNNNGG 3 cut(s) 536, 1154, 1317
AflII CTTAAG 1 cut(s) 1826
AhlI ACTAGT 1 cut(s) 1004
AjnI CCWGG 3 cut(s) 262, 323, 747
Alw21I GWGCWC 3 cut(s) 81, 729, 1534
Alw26I GTCTC 2 cut(s) 876, 1837
AlwI GGATC 6 cut(s) 254, 562, 1104, 1173, 1412, 1934
AlwNI CAGNNNCTG 2 cut(s) 625, 1037
AoxI GGCC 2 cut(s) 1066, 1156
ApeKI GCWGC 7 cut(s) 92, 622, 625, 743, 1523, 1593, 1709
ApoI RAATTY 8 cut(s) 27, 136, 201, 479, 686, 945, 2071, 2226
Asp700I GAANNNNTTC 2 cut(s) 1688, 1956
AspLEI GCGC 2 cut(s) 92, 2169
AspS9I GGNCC 1 cut(s) 1156
AsuHPI GGTGA 6 cut(s) 393, 532, 1022, 1269, 1278, 1905
BalI TGGCCA 1 cut(s) 1068
BanI GGYRCC 1 cut(s) 2023
BanII GRGCYC 2 cut(s) 729, 1364
BauI CACGAG 1 cut(s) 74
BbrPI CACGTG 1 cut(s) 1292
BbsI GAAGAC 1 cut(s) 299
Bbv12I GWGCWC 3 cut(s) 81, 729, 1534
BbvI GCAGC 7 cut(s) 104, 612, 634, 730, 1510, 1580, 1721
BccI CCATC 3 cut(s) 862, 1087, 1186
BciT130I CCWGG 3 cut(s) 264, 325, 749
BclI TGATCA 1 cut(s) 379
BcoDI GTCTC 2 cut(s) 876, 1837
BcuI ACTAGT 1 cut(s) 1004
BfaI CTAG 8 cut(s) 303, 311, 459, 770, 936, 1005, 1472, 1671
BfmI CTRYAG 2 cut(s) 1084, 1423
BfrI CTTAAG 1 cut(s) 1826
BfuAI ACCTGC 1 cut(s) 297
BisI GCNGC 8 cut(s) 93, 96, 623, 626, 744, 1524, 1594, 1710
BlsI GCNGC 8 cut(s) 94, 97, 624, 627, 745, 1525, 1595, 1711
BmcAI AGTACT 1 cut(s) 1551
Bme1390I CCNGG 3 cut(s) 264, 325, 749
BmgT120I GGNCC 1 cut(s) 1156
BmiI GGNNCC 2 cut(s) 981, 2025
BmrFI CCNGG 3 cut(s) 264, 325, 749
BmsI GCATC 4 cut(s) 471, 859, 1134, 1190
BpiI GAAGAC 1 cut(s) 299
BplI GAGNNNNNCTC 2 cut(s) 895, 927
BpmI CTGGAG 2 cut(s) 676, 770
Bpu10I CCTNAGC 1 cut(s) 731
BpuEI CTTGAG 1 cut(s) 705
Bsa29I ATCGAT 1 cut(s) 1860
BsaAI YACGTR 1 cut(s) 1292
BsaBI GATNNNNATC 1 cut(s) 249
BsaI GGTCTC 1 cut(s) 1837
BsaJI CCNNGG 3 cut(s) 195, 747, 1152
BsaXI ACNNNNNCTCC 2 cut(s) 2082, 2112
Bsc4I CCNNNNNNNGG 3 cut(s) 536, 1154, 1317
Bse118I RCCGGY 1 cut(s) 1915
Bse1I ACTGG 2 cut(s) 693, 1569
Bse3DI GCAATG 1 cut(s) 1603
Bse8I GATNNNNATC 1 cut(s) 249
BseBI CCWGG 3 cut(s) 264, 325, 749
BseCI ATCGAT 1 cut(s) 1860
BseDI CCNNGG 3 cut(s) 195, 747, 1152
BseGI GGATG 8 cut(s) 164, 393, 607, 850, 1149, 1168, 1497, 2068
BseJI GATNNNNATC 1 cut(s) 249
BseLI CCNNNNNNNGG 3 cut(s) 536, 1154, 1317
BseMI GCAATG 1 cut(s) 1603
BseMII CTCAG 8 cut(s) 836, 875, 893, 1029, 1068, 1433, 1526, 1848
BseNI ACTGG 2 cut(s) 693, 1569
BseRI GAGGAG 6 cut(s) 158, 849, 864, 1202, 1552, 1900
BseXI GCAGC 7 cut(s) 104, 612, 634, 730, 1510, 1580, 1721
Bsh1236I CGCG 1 cut(s) 2167
Bsh1285I CGRYCG 1 cut(s) 1337
BshFI GGCC 2 cut(s) 1068, 1158
BshNI GGYRCC 1 cut(s) 2023
BshVI ATCGAT 1 cut(s) 1860
BsiEI CGRYCG 1 cut(s) 1337
BsiHKAI GWGCWC 3 cut(s) 81, 729, 1534
BsiSI CCGG 1 cut(s) 1916
BslFI GGGAC 2 cut(s) 1652, 1740
BslI CCNNNNNNNGG 3 cut(s) 536, 1154, 1317
BsmAI GTCTC 2 cut(s) 876, 1837
BsmFI GGGAC 2 cut(s) 1652, 1740
BsmI GAATGC 1 cut(s) 1048
BsnI GGCC 2 cut(s) 1068, 1158
Bso31I GGTCTC 1 cut(s) 1837
Bsp1286I GDGCHC 4 cut(s) 81, 729, 1364, 1534
Bsp1407I TGTACA 2 cut(s) 604, 923
Bsp143I GATC 8 cut(s) 259, 379, 554, 988, 1096, 1165, 1417, 1926
BspACI CCGC 4 cut(s) 96, 1046, 1919, 2102
BspANI GGCC 2 cut(s) 1068, 1158
BspCNI CTCAG 8 cut(s) 837, 874, 894, 1030, 1067, 1434, 1527, 1847
BspDI ATCGAT 1 cut(s) 1860
BspFNI CGCG 1 cut(s) 2167
BspLI GGNNCC 2 cut(s) 981, 2025
BspMI ACCTGC 1 cut(s) 297
BspPI GGATC 6 cut(s) 254, 562, 1104, 1173, 1412, 1934
BspQI GCTCTTC 1 cut(s) 1694
BspT107I GGYRCC 1 cut(s) 2023
BspTI CTTAAG 1 cut(s) 1826
BspTNI GGTCTC 1 cut(s) 1837
BsrDI GCAATG 1 cut(s) 1603
BsrFI RCCGGY 1 cut(s) 1915
BsrGI TGTACA 2 cut(s) 604, 923
BsrI ACTGG 2 cut(s) 693, 1569
BssAI RCCGGY 1 cut(s) 1915
BssECI CCNNGG 3 cut(s) 195, 747, 1152
BssMI GATC 8 cut(s) 259, 379, 554, 988, 1096, 1165, 1417, 1926
BssSI CACGAG 1 cut(s) 74
BssT1I CCWWGG 2 cut(s) 195, 1152
Bst2BI CACGAG 1 cut(s) 74
Bst2UI CCWGG 3 cut(s) 264, 325, 749
Bst4CI ACNGT 8 cut(s) 760, 1182, 1245, 1284, 1335, 1720, 1739, 2174
Bst6I CTCTTC 2 cut(s) 53, 1694
BstAFI CTTAAG 1 cut(s) 1826
BstAUI TGTACA 2 cut(s) 604, 923
BstBAI YACGTR 1 cut(s) 1292
BstC8I GCNNGC 4 cut(s) 703, 831, 1608, 1647
BstENI CCTNNNNNAGG 1 cut(s) 534
BstF5I GGATG 8 cut(s) 164, 393, 607, 850, 1149, 1168, 1497, 2068
BstFNI CGCG 1 cut(s) 2167
BstHHI GCGC 2 cut(s) 92, 2169
BstKTI GATC 8 cut(s) 262, 382, 557, 991, 1099, 1168, 1420, 1929
BstMAI GTCTC 2 cut(s) 876, 1837
BstMBI GATC 8 cut(s) 259, 379, 554, 988, 1096, 1165, 1417, 1926
BstMCI CGRYCG 1 cut(s) 1337
BstMWI GCNNNNNNNGC 6 cut(s) 334, 1235, 1529, 1655, 2164, 2175
BstNI CCWGG 3 cut(s) 264, 325, 749
BstNSI RCATGY 3 cut(s) 705, 1065, 1649
BstSCI CCNGG 3 cut(s) 262, 323, 747
BstSFI CTRYAG 2 cut(s) 1084, 1423
BstUI CGCG 1 cut(s) 2167
BstV1I GCAGC 7 cut(s) 104, 612, 634, 730, 1510, 1580, 1721
BstV2I GAAGAC 1 cut(s) 299
BstX2I RGATCY 3 cut(s) 259, 1096, 1926
BstYI RGATCY 3 cut(s) 259, 1096, 1926
Bsu15I ATCGAT 1 cut(s) 1860
BsuRI GGCC 2 cut(s) 1068, 1158
BsuTUI ATCGAT 1 cut(s) 1860
BtsCI GGATG 8 cut(s) 164, 393, 607, 850, 1149, 1168, 1497, 2068
BtsI GCAGTG 3 cut(s) 1743, 1932, 2038
BtsIMutI CAGTG 6 cut(s) 1280, 1716, 1743, 1744, 1932, 2038
BveI ACCTGC 1 cut(s) 297
Cac8I GCNNGC 4 cut(s) 703, 831, 1608, 1647
CaiI CAGNNNCTG 2 cut(s) 625, 1037
CfoI GCGC 2 cut(s) 92, 2169
Cfr10I RCCGGY 1 cut(s) 1915
Cfr13I GGNCC 1 cut(s) 1156
ClaI ATCGAT 1 cut(s) 1860
Csp6I GTAC 8 cut(s) 286, 314, 580, 605, 924, 1355, 1550, 1906
CspCI CAANNNNNGTGG 2 cut(s) 1732, 1767
CviQI GTAC 8 cut(s) 286, 314, 580, 605, 924, 1355, 1550, 1906
DpnI GATC 8 cut(s) 261, 381, 556, 990, 1098, 1167, 1419, 1928
DpnII GATC 8 cut(s) 259, 379, 554, 988, 1096, 1165, 1417, 1926
DraIII CACNNNGTG 2 cut(s) 1292, 1973
EaeI YGGCCR 1 cut(s) 1066
Eam1104I CTCTTC 2 cut(s) 53, 1694
EarI CTCTTC 2 cut(s) 53, 1694
Ecl136II GAGCTC 1 cut(s) 727
Eco130I CCWWGG 2 cut(s) 195, 1152
Eco24I GRGCYC 2 cut(s) 729, 1364
Eco31I GGTCTC 1 cut(s) 1837
Eco32I GATATC 1 cut(s) 2088
Eco53kI GAGCTC 1 cut(s) 727
Eco57I CTGAAG 6 cut(s) 1108, 1386, 1410, 1500, 1943, 1974
Eco72I CACGTG 1 cut(s) 1292
EcoICRI GAGCTC 1 cut(s) 727
EcoNI CCTNNNNNAGG 1 cut(s) 534
EcoO109I RGGNCCY 1 cut(s) 1156
EcoRI GAATTC 1 cut(s) 2226
EcoRII CCWGG 3 cut(s) 262, 323, 747
EcoRV GATATC 1 cut(s) 2088
EcoT14I CCWWGG 2 cut(s) 195, 1152
EcoT22I ATGCAT 3 cut(s) 1205, 1647, 2135
EcoT38I GRGCYC 2 cut(s) 729, 1364
ErhI CCWWGG 2 cut(s) 195, 1152
FaqI GGGAC 2 cut(s) 1652, 1740
FauI CCCGC 1 cut(s) 2109
FauNDI CATATG 1 cut(s) 1867
FbaI TGATCA 1 cut(s) 379
Fnu4HI GCNGC 8 cut(s) 93, 96, 623, 626, 744, 1524, 1594, 1710
FokI GGATG 8 cut(s) 171, 400, 614, 837, 1155, 1156, 1504, 2075
FriOI GRGCYC 2 cut(s) 729, 1364
Fsp4HI GCNGC 8 cut(s) 93, 96, 623, 626, 744, 1524, 1594, 1710
FspBI CTAG 8 cut(s) 303, 311, 459, 770, 936, 1005, 1472, 1671
GlaI GCGC 2 cut(s) 91, 2168
GluI GCNGC 8 cut(s) 93, 96, 623, 626, 744, 1524, 1594, 1710
GsuI CTGGAG 2 cut(s) 676, 770
HaeIII GGCC 2 cut(s) 1068, 1158
HapII CCGG 1 cut(s) 1916
HhaI GCGC 2 cut(s) 92, 2169
Hin6I GCGC 2 cut(s) 90, 2167
HinP1I GCGC 2 cut(s) 90, 2167
HindIII AAGCTT 5 cut(s) 227, 276, 1130, 1604, 1823
HpaII CCGG 1 cut(s) 1916
HphI GGTGA 6 cut(s) 393, 532, 1022, 1269, 1278, 1905
Hpy166II GTNNAC 3 cut(s) 924, 1051, 1295
Hpy188III TCNNGA 9 cut(s) 530, 656, 722, 809, 941, 960, 1126, 1615, 1961
Hpy8I GTNNAC 3 cut(s) 924, 1051, 1295
HpyAV CCTTC 8 cut(s) 32, 530, 716, 819, 924, 1125, 1762, 1826
HpyCH4III ACNGT 8 cut(s) 760, 1182, 1245, 1284, 1335, 1720, 1739, 2174
HpyCH4IV ACGT 1 cut(s) 1291
HpyF10VI GCNNNNNNNGC 6 cut(s) 334, 1235, 1529, 1655, 2164, 2175
HpySE526I ACGT 1 cut(s) 1291
HspAI GCGC 2 cut(s) 90, 2167
Ksp22I TGATCA 1 cut(s) 379
Kzo9I GATC 8 cut(s) 259, 379, 554, 988, 1096, 1165, 1417, 1926
LguI GCTCTTC 1 cut(s) 1694
LmnI GCTCC 4 cut(s) 732, 979, 1461, 1558
Lsp1109I GCAGC 7 cut(s) 104, 612, 634, 730, 1510, 1580, 1721
LweI GCATC 4 cut(s) 471, 859, 1134, 1190
MaeI CTAG 8 cut(s) 303, 311, 459, 770, 936, 1005, 1472, 1671
MaeII ACGT 1 cut(s) 1291
MalI GATC 8 cut(s) 261, 381, 556, 990, 1098, 1167, 1419, 1928
MboI GATC 8 cut(s) 259, 379, 554, 988, 1096, 1165, 1417, 1926
MfeI CAATTG 1 cut(s) 666
MflI RGATCY 3 cut(s) 259, 1096, 1926
MhlI GDGCHC 4 cut(s) 81, 729, 1364, 1534
MlsI TGGCCA 1 cut(s) 1068
MluNI TGGCCA 1 cut(s) 1068
MlyI GAGTC 5 cut(s) 392, 500, 962, 1046, 1674
MmeI TCCRAC 1 cut(s) 823
Mox20I TGGCCA 1 cut(s) 1068
Mph1103I ATGCAT 3 cut(s) 1205, 1647, 2135
MroXI GAANNNNTTC 2 cut(s) 1688, 1956
MscI TGGCCA 1 cut(s) 1068
MseI TTAA 5 cut(s) 114, 1620, 1697, 1827, 1892
Msp20I TGGCCA 1 cut(s) 1068
MspA1I CMGCKG 1 cut(s) 625
MspCI CTTAAG 1 cut(s) 1826
MspI CCGG 1 cut(s) 1916
MspR9I CCNGG 3 cut(s) 264, 325, 749
MunI CAATTG 1 cut(s) 666
Mva1269I GAATGC 1 cut(s) 1048
MvaI CCWGG 3 cut(s) 264, 325, 749
MvnI CGCG 1 cut(s) 2167
MwoI GCNNNNNNNGC 6 cut(s) 334, 1235, 1529, 1655, 2164, 2175
NdeI CATATG 1 cut(s) 1867
NdeII GATC 8 cut(s) 259, 379, 554, 988, 1096, 1165, 1417, 1926
NlaIV GGNNCC 2 cut(s) 981, 2025
NmuCI GTSAC 5 cut(s) 105, 119, 161, 1275, 1284
NsiI ATGCAT 3 cut(s) 1205, 1647, 2135
NspI RCATGY 3 cut(s) 705, 1065, 1649
PaeI GCATGC 2 cut(s) 705, 1649
PciSI GCTCTTC 1 cut(s) 1694
PctI GAATGC 1 cut(s) 1048
PdmI GAANNNNTTC 2 cut(s) 1688, 1956
PfeI GAWTC 5 cut(s) 434, 473, 1232, 2066, 2192
PflFI GACNNNGTC 1 cut(s) 2151
PfoI TCCNGGA 1 cut(s) 262
PkrI GCNGC 8 cut(s) 94, 97, 624, 627, 745, 1525, 1595, 1711
PleI GAGTC 5 cut(s) 392, 500, 962, 1046, 1673
PmaCI CACGTG 1 cut(s) 1292
PmlI CACGTG 1 cut(s) 1292
PpsI GAGTC 5 cut(s) 392, 500, 962, 1046, 1673
Ppu21I YACGTR 1 cut(s) 1292
PsiI TTATAA 1 cut(s) 893
Psp124BI GAGCTC 1 cut(s) 729
Psp6I CCWGG 3 cut(s) 262, 323, 747
PspCI CACGTG 1 cut(s) 1292
PspGI CCWGG 3 cut(s) 262, 323, 747
PspN4I GGNNCC 2 cut(s) 981, 2025
PspPI GGNCC 1 cut(s) 1156
PsrI GAACNNNNNNTAC 2 cut(s) 588, 620
PstNI CAGNNNCTG 2 cut(s) 625, 1037
PsuI RGATCY 3 cut(s) 259, 1096, 1926
PsyI GACNNNGTC 1 cut(s) 2151
PvuII CAGCTG 1 cut(s) 625
RsaI GTAC 8 cut(s) 287, 315, 581, 606, 925, 1356, 1551, 1907
RsaNI GTAC 8 cut(s) 286, 314, 580, 605, 924, 1355, 1550, 1906
SacI GAGCTC 1 cut(s) 729
SapI GCTCTTC 1 cut(s) 1694
SaqAI TTAA 5 cut(s) 114, 1620, 1697, 1827, 1892
SatI GCNGC 8 cut(s) 93, 96, 623, 626, 744, 1524, 1594, 1710
Sau3AI GATC 8 cut(s) 259, 379, 554, 988, 1096, 1165, 1417, 1926
Sau96I GGNCC 1 cut(s) 1156
ScaI AGTACT 1 cut(s) 1551
SchI GAGTC 5 cut(s) 392, 500, 962, 1046, 1674
ScrFI CCNGG 3 cut(s) 264, 325, 749
SduI GDGCHC 4 cut(s) 81, 729, 1364, 1534
SfaNI GCATC 4 cut(s) 471, 859, 1134, 1190
SfcI CTRYAG 2 cut(s) 1084, 1423
SgrAI CRCCGGYG 1 cut(s) 1915
SmlI CTYRAG 2 cut(s) 720, 1826
SmoI CTYRAG 2 cut(s) 720, 1826
SpeI ACTAGT 1 cut(s) 1004
SphI GCATGC 2 cut(s) 705, 1649
SsiI CCGC 4 cut(s) 96, 1046, 1919, 2102
SspI AATATT 3 cut(s) 449, 739, 1213
SspMI CTAG 8 cut(s) 303, 311, 459, 770, 936, 1005, 1472, 1671
SstI GAGCTC 1 cut(s) 729
StyD4I CCNGG 3 cut(s) 262, 323, 747
StyI CCWWGG 2 cut(s) 195, 1152
TaaI ACNGT 8 cut(s) 760, 1182, 1245, 1284, 1335, 1720, 1739, 2174
TaiI ACGT 1 cut(s) 1294
TaqI TCGA 5 cut(s) 917, 1337, 1432, 1860, 1984
TatI WGTACW 4 cut(s) 604, 923, 1549, 1905
TauI GCSGC 1 cut(s) 98
TfiI GAWTC 5 cut(s) 434, 473, 1232, 2066, 2192
Tru1I TTAA 5 cut(s) 114, 1620, 1697, 1827, 1892
Tru9I TTAA 5 cut(s) 114, 1620, 1697, 1827, 1892
TscAI CASTG 6 cut(s) 1287, 1723, 1744, 1750, 1939, 2045
TseFI GTSAC 5 cut(s) 105, 119, 161, 1275, 1284
TseI GCWGC 7 cut(s) 92, 622, 625, 743, 1523, 1593, 1709
Tsp45I GTSAC 5 cut(s) 105, 119, 161, 1275, 1284
TspGWI ACGGA 2 cut(s) 1677, 1861
TspRI CASTG 6 cut(s) 1287, 1723, 1744, 1750, 1939, 2045
Tth111I GACNNNGTC 1 cut(s) 2151
Vha464I CTTAAG 1 cut(s) 1826
XagI CCTNNNNNAGG 1 cut(s) 534
XapI RAATTY 8 cut(s) 27, 136, 201, 479, 686, 945, 2071, 2226
XceI RCATGY 3 cut(s) 705, 1065, 1649
XcmI CCANNNNNNNNNTGG 1 cut(s) 2056
XmnI GAANNNNTTC 2 cut(s) 1688, 1956
XspI CTAG 8 cut(s) 303, 311, 459, 770, 936, 1005, 1472, 1671
ZrmI AGTACT 1 cut(s) 1551
Zsp2I ATGCAT 3 cut(s) 1205, 1647, 2135
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.