Rmu_sc0000124.1_g000038

Transcription initiation factor TFIID subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000124.1
OG0004540
Physical Location & Seq
Forward (+)
155486 .. 156877
1392 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1392 bp
atggccgccaacatatttggatcacctgatcacatgcctgaccttgttatcgacaaaattcttagagttcttcctactaaagttgccatacaggtgagctttgtttctaaacaatgggaaggtgtaatgtcttcagcccctgcactaaatttcgacgaggatgatgatttagaccgccacaacctcaattgctgcagtgattatctacatcaacacaacaggttcatcaacttcttgcaagggtatgtagaatatcgtgacaacacaaagctcatagacaaactaaggcttcacatgaggatgttcacgtgtcaagatgctgcaaccattaatacgtggttagaactcgctatagagggagatgtgaaagagttagatatcagccctaaaatgagtgttgacccaaaacaatatagagaggatgcgcttagtgagcgctgggtggagagatacgaccactattgctggtaccgcataccttgggtgacccttgttaatgccaaatctttaactagtctaaatctggagtgtatgagagttccggacattcgagctgaggtagacattgaacgtgaccaccccttattcccctttttgaaaaccatgtccttcacagctgtgacgttccatggccatagtttcttccctgtactatggaagtcctcttccatcgagtacttgtcattaactgcatgttcttttgagaatcatgaccctacgtgttacattgaaggttcgagtctcaagtgcttggaagttaagcattgtcatggtcttctgtgtattattgctaacaaagctttcaatctcgaatctcttacatttgtttcaccaaaatgtataagggtgtcaatgtataatacccccaacttgagaaagatcgatatttgtgttgatgaactcggggaattatatttatggcaatgccatcgtgcactaaaggccaccatcaatacccaaaatctcaatctattcagcttctatggttcattggaagcccaagtttttgttaaagcatgttggaaatccataatttcagtttcggaactttgggatgaagaatggttctgttcaaactcgacaaatttgtctactctgatggacttccttaaaggatttggctgcagcaagaaggtgtggctgtgtactcgtgattttaaagctctcatctttccagaaacttttaggaagaccaccggcaccctcgctacaccgttgcctaaaagttgcattctgaatgtggcaatgctgaatcctccaacagcgcgcagtagagactactgggaattgaaggactccatgaattggatagcgcattctgcacggattgttagagataatccccgatggctcgtcaagtacttggaaaaaggcggaaagcaagcaaactag

Protein Analysis

463

Amino Acids

53.57

Weight (kDa)

6.54

Isoelectric Point (pI)

42.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At5g56370 PF24758 162 - 301 1.1e-07 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1087
Acc65I GGTACC 1 cut(s) 468
AccB1I GGYRCC 2 cut(s) 468, 1199
AccB7I CCANNNNNTGG 1 cut(s) 1305
AccI GTMKAC 2 cut(s) 561, 1091
AccII CGCG 1 cut(s) 1267
AccIII TCCGGA 1 cut(s) 541
AciI CCGC 4 cut(s) 6, 175, 472, 1374
AclWI GGATC 1 cut(s) 28
AcoI YGGCCR 2 cut(s) 3, 631
AcsI RAATTY 3 cut(s) 57, 148, 1084
AcuI CTGAAG 1 cut(s) 117
AcvI CACGTG 1 cut(s) 309
AfaI GTAC 5 cut(s) 470, 651, 677, 1147, 1361
AfeI AGCGCT 1 cut(s) 437
AfiI CCNNNNNNNGG 1 cut(s) 1305
AflIII ACRYGT 2 cut(s) 308, 719
AgsI TTSAA 6 cut(s) 569, 598, 731, 805, 1074, 1291
AhlI ACTAGT 1 cut(s) 512
AleI CACNNNNGTG 1 cut(s) 617
AluBI AGCT 7 cut(s) 99, 271, 554, 617, 800, 978, 1163
AluI AGCT 7 cut(s) 99, 271, 554, 617, 800, 978, 1163
Alw21I GWGCWC 1 cut(s) 937
Alw26I GTCTC 2 cut(s) 746, 1269
Alw44I GTGCAC 1 cut(s) 933
AlwI GGATC 1 cut(s) 28
AlwNI CAGNNNCTG 1 cut(s) 140
Ama87I CYCGRG 1 cut(s) 902
Aor13HI TCCGGA 1 cut(s) 541
Aor51HI AGCGCT 1 cut(s) 437
AoxI GGCC 3 cut(s) 3, 631, 942
ApaLI GTGCAC 1 cut(s) 933
ApeKI GCWGC 4 cut(s) 192, 320, 1122, 1125
ApoI RAATTY 3 cut(s) 57, 148, 1084
AseI ATTAAT 1 cut(s) 330
Asp718I GGTACC 1 cut(s) 468
AspLEI GCGC 5 cut(s) 427, 438, 1267, 1269, 1315
AsuHPI GGTGA 4 cut(s) 15, 106, 496, 822
AvaI CYCGRG 1 cut(s) 902
BaeGI GKGCMC 1 cut(s) 937
BalI TGGCCA 1 cut(s) 633
BanI GGYRCC 2 cut(s) 468, 1199
BauI CACGAG 1 cut(s) 1149
BbrPI CACGTG 1 cut(s) 309
BbsI GAAGAC 3 cut(s) 123, 767, 1196
Bbv12I GWGCWC 1 cut(s) 937
BbvCI CCTCAGC 1 cut(s) 555
BbvI GCAGC 4 cut(s) 179, 307, 1109, 1137
BccI CCATC 5 cut(s) 677, 936, 956, 1093, 1341
BcgI CGANNNNNNTGC 2 cut(s) 911, 945
BclI TGATCA 1 cut(s) 28
BcoDI GTCTC 2 cut(s) 746, 1269
BcuI ACTAGT 1 cut(s) 512
BfaI CTAG 2 cut(s) 513, 1390
BfmI CTRYAG 3 cut(s) 193, 351, 1123
BfoI RGCGCY 1 cut(s) 439
BisI GCNGC 5 cut(s) 6, 193, 321, 1123, 1126
BlsI GCNGC 5 cut(s) 7, 194, 322, 1124, 1127
BmcAI AGTACT 2 cut(s) 677, 1361
BmeT110I CYCGRG 1 cut(s) 902
BmiI GGNNCC 2 cut(s) 470, 1201
BmrI ACTGGG 1 cut(s) 1291
BmsI GCATC 2 cut(s) 307, 412
BmuI ACTGGG 1 cut(s) 1291
BpiI GAAGAC 3 cut(s) 123, 767, 1196
BpmI CTGGAG 1 cut(s) 545
Bpu10I CCTNAGC 1 cut(s) 555
BpuEI CTTGAG 2 cut(s) 728, 892
Bsa29I ATCGAT 1 cut(s) 882
BsaAI YACGTR 3 cut(s) 309, 336, 720
BsaJI CCNNGG 2 cut(s) 479, 628
BsaWI WCCGGW 1 cut(s) 541
Bsc4I CCNNNNNNNGG 1 cut(s) 1305
Bse118I RCCGGY 1 cut(s) 1196
Bse1I ACTGG 1 cut(s) 1286
Bse3DI GCAATG 2 cut(s) 929, 1251
BseAI TCCGGA 1 cut(s) 541
BseCI ATCGAT 1 cut(s) 882
BseDI CCNNGG 2 cut(s) 479, 628
BseGI GGATG 4 cut(s) 166, 306, 427, 1060
BseLI CCNNNNNNNGG 1 cut(s) 1305
BseMI GCAATG 2 cut(s) 929, 1251
BseMII CTCAG 1 cut(s) 546
BseNI ACTGG 1 cut(s) 1286
BsePI GCGCGC 1 cut(s) 1265
BseSI GKGCMC 1 cut(s) 937
BseXI GCAGC 4 cut(s) 179, 307, 1109, 1137
BseYI CCCAGC 1 cut(s) 438
BsgI GTGCAG 2 cut(s) 126, 1305
Bsh1236I CGCG 1 cut(s) 1267
BshFI GGCC 3 cut(s) 5, 633, 944
BshNI GGYRCC 2 cut(s) 468, 1199
BshVI ATCGAT 1 cut(s) 882
BsiHKAI GWGCWC 1 cut(s) 937
BsiHKCI CYCGRG 1 cut(s) 902
BsiSI CCGG 2 cut(s) 542, 1197
BslI CCNNNNNNNGG 1 cut(s) 1305
BsmAI GTCTC 2 cut(s) 746, 1269
BsmI GAATGC 2 cut(s) 1230, 1315
BsnI GGCC 3 cut(s) 5, 633, 944
BsoBI CYCGRG 1 cut(s) 902
Bsp1286I GDGCHC 1 cut(s) 937
Bsp13I TCCGGA 1 cut(s) 541
Bsp143I GATC 3 cut(s) 20, 28, 879
Bsp19I CCATGG 1 cut(s) 628
BspACI CCGC 4 cut(s) 6, 175, 472, 1374
BspANI GGCC 3 cut(s) 5, 633, 944
BspCNI CTCAG 1 cut(s) 547
BspDI ATCGAT 1 cut(s) 882
BspEI TCCGGA 1 cut(s) 541
BspFNI CGCG 1 cut(s) 1267
BspHI TCATGA 1 cut(s) 709
BspLI GGNNCC 2 cut(s) 470, 1201
BspMAI CTGCAG 2 cut(s) 197, 1127
BspPI GGATC 1 cut(s) 28
BspT107I GGYRCC 2 cut(s) 468, 1199
BsrDI GCAATG 2 cut(s) 929, 1251
BsrFI RCCGGY 1 cut(s) 1196
BsrI ACTGG 1 cut(s) 1286
BssAI RCCGGY 1 cut(s) 1196
BssECI CCNNGG 2 cut(s) 479, 628
BssHII GCGCGC 1 cut(s) 1265
BssMI GATC 3 cut(s) 20, 28, 879
BssSI CACGAG 1 cut(s) 1149
BssT1I CCWWGG 2 cut(s) 479, 628
Bst2BI CACGAG 1 cut(s) 1149
Bst4CI ACNGT 1 cut(s) 1215
Bst6I CTCTTC 1 cut(s) 670
BstBAI YACGTR 3 cut(s) 309, 336, 720
BstC8I GCNNGC 2 cut(s) 1267, 1383
BstDEI CTNAG 4 cut(s) 62, 284, 428, 555
BstDSI CCRYGG 1 cut(s) 628
BstEII GGTNACC 1 cut(s) 484
BstF5I GGATG 4 cut(s) 166, 306, 427, 1060
BstFNI CGCG 1 cut(s) 1267
BstH2I RGCGCY 1 cut(s) 439
BstHHI GCGC 5 cut(s) 427, 438, 1267, 1269, 1315
BstKTI GATC 3 cut(s) 23, 31, 882
BstMAI GTCTC 2 cut(s) 746, 1269
BstMBI GATC 3 cut(s) 20, 28, 879
BstMWI GCNNNNNNNGC 5 cut(s) 433, 471, 797, 941, 1319
BstNSI RCATGY 3 cut(s) 37, 696, 1020
BstPI GGTNACC 1 cut(s) 484
BstSFI CTRYAG 3 cut(s) 193, 351, 1123
BstSLI GKGCMC 1 cut(s) 937
BstUI CGCG 1 cut(s) 1267
BstV1I GCAGC 4 cut(s) 179, 307, 1109, 1137
BstV2I GAAGAC 3 cut(s) 123, 767, 1196
Bsu15I ATCGAT 1 cut(s) 882
BsuRI GGCC 3 cut(s) 5, 633, 944
BsuTUI ATCGAT 1 cut(s) 882
BtgI CCRYGG 1 cut(s) 628
BtsCI GGATG 4 cut(s) 166, 306, 427, 1060
BtsI GCAGTG 1 cut(s) 202
BtsIMutI CAGTG 1 cut(s) 202
Cac8I GCNNGC 2 cut(s) 1267, 1383
CaiI CAGNNNCTG 1 cut(s) 140
CciI TCATGA 1 cut(s) 709
CfoI GCGC 5 cut(s) 427, 438, 1267, 1269, 1315
Cfr10I RCCGGY 1 cut(s) 1196
ClaI ATCGAT 1 cut(s) 882
Csp6I GTAC 5 cut(s) 469, 650, 676, 1146, 1360
CviAII CATG 9 cut(s) 34, 295, 604, 629, 693, 710, 770, 1017, 1300
CviQI GTAC 5 cut(s) 469, 650, 676, 1146, 1360
DdeI CTNAG 4 cut(s) 62, 284, 428, 555
DpnI GATC 3 cut(s) 22, 30, 881
DpnII GATC 3 cut(s) 20, 28, 879
DraI TTTAAA 1 cut(s) 1159
DrdI GACNNNNNNGTC 1 cut(s) 1087
DseDI GACNNNNNNGTC 1 cut(s) 1087
EaeI YGGCCR 2 cut(s) 3, 631
Eam1104I CTCTTC 1 cut(s) 670
EarI CTCTTC 1 cut(s) 670
EciI GGCGGA 1 cut(s) 1389
Eco130I CCWWGG 2 cut(s) 479, 628
Eco32I GATATC 1 cut(s) 379
Eco47III AGCGCT 1 cut(s) 437
Eco57I CTGAAG 1 cut(s) 117
Eco72I CACGTG 1 cut(s) 309
Eco88I CYCGRG 1 cut(s) 902
Eco91I GGTNACC 1 cut(s) 484
EcoO65I GGTNACC 1 cut(s) 484
EcoRV GATATC 1 cut(s) 379
EcoT14I CCWWGG 2 cut(s) 479, 628
ErhI CCWWGG 2 cut(s) 479, 628
FaeI CATG 9 cut(s) 37, 298, 607, 632, 696, 713, 773, 1020, 1303
FatI CATG 9 cut(s) 33, 294, 603, 628, 692, 709, 769, 1016, 1299
FbaI TGATCA 1 cut(s) 28
FblI GTMKAC 2 cut(s) 561, 1091
Fnu4HI GCNGC 5 cut(s) 6, 193, 321, 1123, 1126
FokI GGATG 4 cut(s) 173, 313, 434, 1067
Fsp4HI GCNGC 5 cut(s) 6, 193, 321, 1123, 1126
FspBI CTAG 2 cut(s) 513, 1390
GlaI GCGC 5 cut(s) 426, 437, 1266, 1268, 1314
GluI GCNGC 5 cut(s) 6, 193, 321, 1123, 1126
GsaI CCCAGC 1 cut(s) 442
GsuI CTGGAG 1 cut(s) 545
HaeII RGCGCY 1 cut(s) 439
HaeIII GGCC 3 cut(s) 5, 633, 944
HapII CCGG 2 cut(s) 542, 1197
HhaI GCGC 5 cut(s) 427, 438, 1267, 1269, 1315
Hin1II CATG 9 cut(s) 37, 298, 607, 632, 696, 713, 773, 1020, 1303
Hin6I GCGC 5 cut(s) 425, 436, 1265, 1267, 1313
HinP1I GCGC 5 cut(s) 425, 436, 1265, 1267, 1313
HincII GTYRAC 1 cut(s) 400
HindII GTYRAC 1 cut(s) 400
HindIII AAGCTT 1 cut(s) 798
HinfI GANTC 5 cut(s) 706, 739, 812, 1252, 1295
HpaII CCGG 2 cut(s) 542, 1197
HphI GGTGA 4 cut(s) 15, 106, 496, 822
Hpy166II GTNNAC 6 cut(s) 306, 400, 562, 935, 1092, 1146
Hpy188I TCNGA 3 cut(s) 1045, 1098, 1236
Hpy188III TCNNGA 8 cut(s) 257, 314, 524, 542, 710, 809, 1151, 1175
Hpy8I GTNNAC 6 cut(s) 306, 400, 562, 935, 1092, 1146
Hpy99I CGWCG 1 cut(s) 158
HpyAV CCTTC 5 cut(s) 113, 619, 725, 1126, 1285
HpyCH4III ACNGT 1 cut(s) 1215
HpyCH4IV ACGT 5 cut(s) 308, 335, 571, 623, 719
HpyCH4V TGCA 9 cut(s) 143, 195, 238, 323, 692, 935, 1125, 1230, 1322
HpyF10VI GCNNNNNNNGC 5 cut(s) 433, 471, 797, 941, 1319
HpyF3I CTNAG 4 cut(s) 62, 284, 428, 555
HpySE526I ACGT 5 cut(s) 308, 335, 571, 623, 719
Hsp92II CATG 9 cut(s) 37, 298, 607, 632, 696, 713, 773, 1020, 1303
HspAI GCGC 5 cut(s) 425, 436, 1265, 1267, 1313
Kpn2I TCCGGA 1 cut(s) 541
KpnI GGTACC 1 cut(s) 472
Ksp22I TGATCA 1 cut(s) 28
Kzo9I GATC 3 cut(s) 20, 28, 879
Lsp1109I GCAGC 4 cut(s) 179, 307, 1109, 1137
LweI GCATC 2 cut(s) 307, 412
MaeI CTAG 2 cut(s) 513, 1390
MaeII ACGT 5 cut(s) 308, 335, 571, 623, 719
MaeIII GTNAC 5 cut(s) 257, 484, 572, 619, 722
MalI GATC 3 cut(s) 22, 30, 881
MboI GATC 3 cut(s) 20, 28, 879
MboII GAAGA 7 cut(s) 62, 123, 634, 657, 767, 1070, 1201
MfeI CAATTG 1 cut(s) 187
MhlI GDGCHC 1 cut(s) 937
MlsI TGGCCA 1 cut(s) 633
MluCI AATT 8 cut(s) 57, 148, 187, 908, 1032, 1084, 1286, 1303
MluNI TGGCCA 1 cut(s) 633
MlyI GAGTC 2 cut(s) 748, 1289
MmeI TCCRAC 2 cut(s) 1001, 1283
MnlI CCTC 9 cut(s) 151, 194, 291, 349, 412, 550, 673, 1214, 1266
Mox20I TGGCCA 1 cut(s) 633
MroI TCCGGA 1 cut(s) 541
MscI TGGCCA 1 cut(s) 633
MseI TTAA 8 cut(s) 330, 495, 509, 686, 759, 1011, 1110, 1158
MslI CAYNNNNRTG 5 cut(s) 92, 299, 617, 768, 835
Msp20I TGGCCA 1 cut(s) 633
MspA1I CMGCKG 1 cut(s) 617
MspI CCGG 2 cut(s) 542, 1197
MunI CAATTG 1 cut(s) 187
Mva1269I GAATGC 2 cut(s) 1230, 1315
MvnI CGCG 1 cut(s) 1267
MwoI GCNNNNNNNGC 5 cut(s) 433, 471, 797, 941, 1319
NcoI CCATGG 1 cut(s) 628
NdeII GATC 3 cut(s) 20, 28, 879
NlaIII CATG 9 cut(s) 37, 298, 607, 632, 696, 713, 773, 1020, 1303
NlaIV GGNNCC 2 cut(s) 470, 1201
NmuCI GTSAC 4 cut(s) 257, 484, 572, 619
NspI RCATGY 3 cut(s) 37, 696, 1020
OliI CACNNNNGTG 1 cut(s) 617
PagI TCATGA 1 cut(s) 709
PauI GCGCGC 1 cut(s) 1265
PctI GAATGC 2 cut(s) 1230, 1315
PfeI GAWTC 3 cut(s) 706, 812, 1252
PflMI CCANNNNNTGG 1 cut(s) 1305
PkrI GCNGC 5 cut(s) 7, 194, 322, 1124, 1127
PleI GAGTC 2 cut(s) 747, 1289
PmaCI CACGTG 1 cut(s) 309
PmlI CACGTG 1 cut(s) 309
PpsI GAGTC 2 cut(s) 747, 1289
Ppu21I YACGTR 3 cut(s) 309, 336, 720
PshBI ATTAAT 1 cut(s) 330
PspCI CACGTG 1 cut(s) 309
PspEI GGTNACC 1 cut(s) 484
PspFI CCCAGC 1 cut(s) 438
PspN4I GGNNCC 2 cut(s) 470, 1201
PstI CTGCAG 2 cut(s) 197, 1127
PstNI CAGNNNCTG 1 cut(s) 140
PteI GCGCGC 1 cut(s) 1265
PvuII CAGCTG 1 cut(s) 617
RsaI GTAC 5 cut(s) 470, 651, 677, 1147, 1361
RsaNI GTAC 5 cut(s) 469, 650, 676, 1146, 1360
RseI CAYNNNNRTG 5 cut(s) 92, 299, 617, 768, 835
SaqAI TTAA 8 cut(s) 330, 495, 509, 686, 759, 1011, 1110, 1158
SatI GCNGC 5 cut(s) 6, 193, 321, 1123, 1126
Sau3AI GATC 3 cut(s) 20, 28, 879
ScaI AGTACT 2 cut(s) 677, 1361
SchI GAGTC 2 cut(s) 748, 1289
SduI GDGCHC 1 cut(s) 937
SfaNI GCATC 2 cut(s) 307, 412
SfcI CTRYAG 3 cut(s) 193, 351, 1123
SmiMI CAYNNNNRTG 5 cut(s) 92, 299, 617, 768, 835
SmlI CTYRAG 2 cut(s) 743, 871
SmoI CTYRAG 2 cut(s) 743, 871
SpeI ACTAGT 1 cut(s) 512
Sse9I AATT 8 cut(s) 57, 148, 187, 908, 1032, 1084, 1286, 1303
SsiI CCGC 4 cut(s) 6, 175, 472, 1374
SspMI CTAG 2 cut(s) 513, 1390
StyI CCWWGG 2 cut(s) 479, 628
TaaI ACNGT 1 cut(s) 1215
TaiI ACGT 5 cut(s) 311, 338, 574, 626, 722
TaqI TCGA 8 cut(s) 51, 153, 550, 672, 737, 810, 882, 1079
TasI AATT 8 cut(s) 57, 148, 187, 908, 1032, 1084, 1286, 1303
TatI WGTACW 4 cut(s) 649, 675, 1145, 1359
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 3 cut(s) 706, 812, 1252
Tru1I TTAA 8 cut(s) 330, 495, 509, 686, 759, 1011, 1110, 1158
Tru9I TTAA 8 cut(s) 330, 495, 509, 686, 759, 1011, 1110, 1158
TscAI CASTG 1 cut(s) 202
TseFI GTSAC 4 cut(s) 257, 484, 572, 619
TseI GCWGC 4 cut(s) 192, 320, 1122, 1125
Tsp45I GTSAC 4 cut(s) 257, 484, 572, 619
TspDTI ATGAA 5 cut(s) 214, 912, 978, 1071, 1316
TspGWI ACGGA 1 cut(s) 1339
TspRI CASTG 1 cut(s) 202
Van91I CCANNNNNTGG 1 cut(s) 1305
VneI GTGCAC 1 cut(s) 933
VspI ATTAAT 1 cut(s) 330
XapI RAATTY 3 cut(s) 57, 148, 1084
XceI RCATGY 3 cut(s) 37, 696, 1020
XmiI GTMKAC 2 cut(s) 561, 1091
XspI CTAG 2 cut(s) 513, 1390
ZrmI AGTACT 2 cut(s) 677, 1361
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.