Rmu_sc0001639.1_g000031

PWWP domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001639.1
OG0002940
Physical Location & Seq
Reverse (-)
131389 .. 133678
2290 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1719 bp
atggatgggaacatgtttagtgatgtgaatggccaatatgatccaaaaccggaattccatgtttatgatatagtatgggggaaagtaaggggttatccctggtggcctggacggatatgtgatccttcagcttcatcgaatgaggcaaagatgcttttcagaaagggtagttatttgatagattattttggggatcactcatttacttggaatgaagcaccacatattaagccctttctggattatttctcacaaatggagaagcagagtaataaggacgaattccaaaatgccattgcttgtgctctggacgaattcttaagacgggtagagttgggggtggtctgttcttgcatatctggagaggtatatccaaaactcaaaaatcagataattattagtgatggagtcaagcaagaagaagaagaagaagaagaagaagaagaagacagtggaaatgctggtgacggttcccttaccgccgtctcttttgagcctcagaaactcattatgcttctaaaggagtttgctcagttttcatatggaaaagctgaccaactagaactttcaacatcagtgatccaattatcagctttctattgttggaagagttctcagctgtcgctgccaacggagaatgatgcctatatatcagtattgggggagaagactcgatgcaatgtattaggtcacaattcacttccagtaactacagatgagaagctggtgcagaagtctaaaagtaaagctaacaacaatgatctgggatgttgtgcaacattatggctggtgaagaagcaacgcacaattcacacccgcaggaataaaatcctggttccgcccttgtgtttgtctattccaatgactgataatgcagcagaaggggaagctagtggtttggttgttttaccttccatgagtgagaatacatcagaaggggaaactagaagtaaattgattttgcaatcttctgatgagaatccagaggaagttgatgctatagcggatgaagatgtgacgacagtaagtgaagctgcacttccagtaacaaaggatgtgaaattggtgtggaaatcagaaggtaaagataactcattgcacaagtgtaatctcatgtctgagtgcagtatgaattcgagtgaaaaggagaaaatattgtcagatttaaaggcttataagtgtttgtctactccaatgtgtcagaacggattggaagggaaagcaggcagtaaattgactttgcaatcctgtgtaaagcgcaaggcagttgatgttatggctgatgactttgcgctgaaacataggaaaagtgattcatcaacaaggactaaagatgcacttctgctaactgaggatgagatgttagtgaggaaaaccgaaagtaaaaggaactcgatgcataagtgcaagcgcatatatagagataaaatgaatccaagtaaagaacagaaaagcttatcggatttaaatggtgagaagtgtttgtctccaacaagtgagtatagattaccttctgatcatacattgactttgcactcctctagtagtatacaaaaggaagttgatcctatagctgatgactctgcactggaagattgggaaagctatttatcagcaggggatgttaagcagtcttttggagtcgcagattttattaacatgtttctacagagggagcacacttctggagctggagaaaggatctgtctggttgaatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PWWP

Protein Analysis

572

Amino Acids

64.01

Weight (kDa)

5.12

Isoelectric Point (pI)

48.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PWWP PF00855 23 - 105 7.4e-19 PWWP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1176
AccI GTMKAC 2 cut(s) 1187, 1549
AciI CCGC 4 cut(s) 480, 817, 839, 1004
AclWI GGATC 6 cut(s) 35, 116, 201, 574, 1559, 1709
AcoI YGGCCR 1 cut(s) 31
AcsI RAATTY 4 cut(s) 53, 281, 314, 1132
AcuI CTGAAG 1 cut(s) 111
AflII CTTAAG 1 cut(s) 319
AflIII ACRYGT 2 cut(s) 12, 1659
AgsI TTSAA 2 cut(s) 570, 1715
AjnI CCWGG 3 cut(s) 98, 106, 831
Alw21I GWGCWC 2 cut(s) 307, 1680
Alw26I GTCTC 2 cut(s) 490, 1491
AlwI GGATC 6 cut(s) 35, 116, 201, 574, 1559, 1709
AoxI GGCC 2 cut(s) 31, 104
ApeKI GCWGC 3 cut(s) 625, 875, 1034
ApoI RAATTY 4 cut(s) 53, 281, 314, 1132
AspLEI GCGC 3 cut(s) 1260, 1294, 1413
AsuHPI GGTGA 3 cut(s) 476, 802, 1484
BalI TGGCCA 1 cut(s) 33
BbsI GAAGAC 2 cut(s) 453, 674
Bbv12I GWGCWC 2 cut(s) 307, 1680
BbvI GCAGC 3 cut(s) 612, 887, 1021
BccI CCATC 1 cut(s) 398
BceAI ACGGC 1 cut(s) 467
BciT130I CCWGG 3 cut(s) 100, 108, 833
BclI TGATCA 1 cut(s) 1516
BcoDI GTCTC 2 cut(s) 490, 1491
BfaI CTAG 4 cut(s) 560, 891, 945, 1542
BfmI CTRYAG 4 cut(s) 711, 999, 1569, 1667
BfrI CTTAAG 1 cut(s) 319
BisI GCNGC 3 cut(s) 626, 876, 1035
BlsI GCNGC 3 cut(s) 627, 877, 1036
Bme1390I CCNGG 3 cut(s) 100, 108, 833
BmiI GGNNCC 2 cut(s) 472, 837
BmrFI CCNGG 3 cut(s) 100, 108, 833
BmsI GCATC 6 cut(s) 141, 631, 665, 985, 1324, 1386
BpiI GAAGAC 2 cut(s) 453, 674
BpmI CTGGAG 3 cut(s) 381, 1707, 1713
BsaBI GATNNNNATC 1 cut(s) 978
BsaJI CCNNGG 1 cut(s) 98
BsaWI WCCGGW 1 cut(s) 49
Bse1I ACTGG 3 cut(s) 704, 1043, 1593
Bse3DI GCAATG 3 cut(s) 294, 685, 1094
Bse8I GATNNNNATC 1 cut(s) 978
BseBI CCWGG 3 cut(s) 100, 108, 833
BseDI CCNNGG 1 cut(s) 98
BseGI GGATG 6 cut(s) 10, 773, 1012, 1060, 1360, 1627
BseJI GATNNNNATC 1 cut(s) 978
BseMI GCAATG 3 cut(s) 294, 685, 1094
BseMII CTCAG 5 cut(s) 512, 545, 629, 1110, 1341
BseNI ACTGG 3 cut(s) 704, 1043, 1593
BseRI GAGGAG 1 cut(s) 1528
BseXI GCAGC 3 cut(s) 612, 887, 1021
BsgI GTGCAG 4 cut(s) 749, 1020, 1144, 1569
BshFI GGCC 2 cut(s) 33, 106
BsiHKAI GWGCWC 2 cut(s) 307, 1680
BsiSI CCGG 1 cut(s) 50
BsmAI GTCTC 2 cut(s) 490, 1491
BsmBI CGTCTC 1 cut(s) 490
BsnI GGCC 2 cut(s) 33, 106
Bsp1286I GDGCHC 2 cut(s) 307, 1680
Bsp143I GATC 8 cut(s) 40, 121, 193, 579, 760, 1516, 1564, 1701
BspACI CCGC 4 cut(s) 480, 817, 839, 1004
BspANI GGCC 2 cut(s) 33, 106
BspCNI CTCAG 5 cut(s) 511, 544, 628, 1111, 1342
BspLI GGNNCC 2 cut(s) 472, 837
BspPI GGATC 6 cut(s) 35, 116, 201, 574, 1559, 1709
BspTI CTTAAG 1 cut(s) 319
BsrDI GCAATG 3 cut(s) 294, 685, 1094
BsrI ACTGG 3 cut(s) 704, 1043, 1593
BssECI CCNNGG 1 cut(s) 98
BssMI GATC 8 cut(s) 40, 121, 193, 579, 760, 1516, 1564, 1701
BssNAI GTATAC 1 cut(s) 1550
Bst1107I GTATAC 1 cut(s) 1550
Bst2UI CCWGG 3 cut(s) 100, 108, 833
Bst4CI ACNGT 3 cut(s) 452, 470, 1024
Bst6I CTCTTC 1 cut(s) 602
BstAFI CTTAAG 1 cut(s) 319
BstC8I GCNNGC 2 cut(s) 1225, 1409
BstDEI CTNAG 5 cut(s) 498, 531, 615, 1119, 1350
BstF5I GGATG 6 cut(s) 10, 773, 1012, 1060, 1360, 1627
BstHHI GCGC 3 cut(s) 1260, 1294, 1413
BstKTI GATC 8 cut(s) 43, 124, 196, 582, 763, 1519, 1567, 1704
BstMAI GTCTC 2 cut(s) 490, 1491
BstMBI GATC 8 cut(s) 40, 121, 193, 579, 760, 1516, 1564, 1701
BstMWI GCNNNNNNNGC 1 cut(s) 625
BstNI CCWGG 3 cut(s) 100, 108, 833
BstNSI RCATGY 2 cut(s) 16, 1663
BstSCI CCNGG 3 cut(s) 98, 106, 831
BstSFI CTRYAG 4 cut(s) 711, 999, 1569, 1667
BstV1I GCAGC 3 cut(s) 612, 887, 1021
BstV2I GAAGAC 2 cut(s) 453, 674
BstX2I RGATCY 1 cut(s) 1701
BstYI RGATCY 1 cut(s) 1701
BstZ17I GTATAC 1 cut(s) 1550
BsuRI GGCC 2 cut(s) 33, 106
BtsCI GGATG 6 cut(s) 10, 773, 1012, 1060, 1360, 1627
BtsIMutI CAGTG 3 cut(s) 457, 582, 1586
Cac8I GCNNGC 2 cut(s) 1225, 1409
CfoI GCGC 3 cut(s) 1260, 1294, 1413
CviAII CATG 5 cut(s) 13, 59, 916, 1114, 1660
DdeI CTNAG 5 cut(s) 498, 531, 615, 1119, 1350
DpnI GATC 8 cut(s) 42, 123, 195, 581, 762, 1518, 1566, 1703
DpnII GATC 8 cut(s) 40, 121, 193, 579, 760, 1516, 1564, 1701
DraI TTTAAA 2 cut(s) 1167, 1467
EaeI YGGCCR 1 cut(s) 31
Eam1104I CTCTTC 1 cut(s) 602
EarI CTCTTC 1 cut(s) 602
EciI GGCGGA 1 cut(s) 828
Eco57I CTGAAG 1 cut(s) 111
EcoRI GAATTC 4 cut(s) 53, 281, 314, 1132
EcoRII CCWGG 3 cut(s) 98, 106, 831
EcoT22I ATGCAT 1 cut(s) 1401
Esp3I CGTCTC 1 cut(s) 490
FaeI CATG 5 cut(s) 16, 62, 919, 1117, 1663
FalI AAGNNNNNCTT 4 cut(s) 1023, 1055, 1323, 1355
FatI CATG 5 cut(s) 12, 58, 915, 1113, 1659
FauI CCCGC 1 cut(s) 824
FauNDI CATATG 1 cut(s) 541
FbaI TGATCA 1 cut(s) 1516
FblI GTMKAC 2 cut(s) 1187, 1549
Fnu4HI GCNGC 3 cut(s) 626, 876, 1035
FokI GGATG 6 cut(s) 17, 780, 1019, 1067, 1367, 1634
Fsp4HI GCNGC 3 cut(s) 626, 876, 1035
FspBI CTAG 4 cut(s) 560, 891, 945, 1542
GlaI GCGC 3 cut(s) 1259, 1293, 1412
GluI GCNGC 3 cut(s) 626, 876, 1035
GsuI CTGGAG 3 cut(s) 381, 1707, 1713
HaeIII GGCC 2 cut(s) 33, 106
HapII CCGG 1 cut(s) 50
HhaI GCGC 3 cut(s) 1260, 1294, 1413
Hin1II CATG 5 cut(s) 16, 62, 919, 1117, 1663
Hin6I GCGC 3 cut(s) 1258, 1292, 1411
HinP1I GCGC 3 cut(s) 1258, 1292, 1411
HindIII AAGCTT 1 cut(s) 1453
HinfI GANTC 7 cut(s) 408, 670, 979, 1313, 1432, 1580, 1641
HpaII CCGG 1 cut(s) 50
HphI GGTGA 3 cut(s) 476, 802, 1484
Hpy166II GTNNAC 2 cut(s) 1188, 1550
Hpy188III TCNNGA 5 cut(s) 239, 308, 360, 983, 1686
Hpy8I GTNNAC 2 cut(s) 1188, 1550
HpyAV CCTTC 7 cut(s) 135, 875, 921, 929, 1073, 1208, 1521
HpyCH4III ACNGT 3 cut(s) 452, 470, 1024
HpyF10VI GCNNNNNNNGC 1 cut(s) 625
HpyF3I CTNAG 5 cut(s) 498, 531, 615, 1119, 1350
Hsp92II CATG 5 cut(s) 16, 62, 919, 1117, 1663
HspAI GCGC 3 cut(s) 1258, 1292, 1411
Ksp22I TGATCA 1 cut(s) 1516
Kzo9I GATC 8 cut(s) 40, 121, 193, 579, 760, 1516, 1564, 1701
LmnI GCTCC 2 cut(s) 1675, 1688
Lsp1109I GCAGC 3 cut(s) 612, 887, 1021
LweI GCATC 6 cut(s) 141, 631, 665, 985, 1324, 1386
MaeI CTAG 4 cut(s) 560, 891, 945, 1542
MaeIII GTNAC 5 cut(s) 464, 689, 706, 1015, 1045
MalI GATC 8 cut(s) 42, 123, 195, 581, 762, 1518, 1566, 1703
MboI GATC 8 cut(s) 40, 121, 193, 579, 760, 1516, 1564, 1701
MflI RGATCY 1 cut(s) 1701
MhlI GDGCHC 2 cut(s) 307, 1680
MlsI TGGCCA 1 cut(s) 33
MluNI TGGCCA 1 cut(s) 33
MlyI GAGTC 4 cut(s) 417, 664, 1574, 1650
MmeI TCCRAC 2 cut(s) 584, 1514
MnlI CCTC 8 cut(s) 136, 358, 507, 979, 1345, 1362, 1549, 1665
Mox20I TGGCCA 1 cut(s) 33
Mph1103I ATGCAT 1 cut(s) 1401
MscI TGGCCA 1 cut(s) 33
MseI TTAA 6 cut(s) 228, 320, 1166, 1466, 1626, 1656
MslI CAYNNNNRTG 1 cut(s) 63
Msp20I TGGCCA 1 cut(s) 33
MspA1I CMGCKG 1 cut(s) 619
MspCI CTTAAG 1 cut(s) 319
MspI CCGG 1 cut(s) 50
MspR9I CCNGG 3 cut(s) 100, 108, 833
MvaI CCWGG 3 cut(s) 100, 108, 833
MwoI GCNNNNNNNGC 1 cut(s) 625
NdeI CATATG 1 cut(s) 541
NdeII GATC 8 cut(s) 40, 121, 193, 579, 760, 1516, 1564, 1701
NlaIII CATG 5 cut(s) 16, 62, 919, 1117, 1663
NlaIV GGNNCC 2 cut(s) 472, 837
NmuCI GTSAC 3 cut(s) 464, 689, 1015
NsiI ATGCAT 1 cut(s) 1401
NspI RCATGY 2 cut(s) 16, 1663
PciI ACATGT 2 cut(s) 12, 1659
PfeI GAWTC 3 cut(s) 979, 1313, 1432
PkrI GCNGC 3 cut(s) 627, 877, 1036
PleI GAGTC 4 cut(s) 416, 664, 1574, 1649
PpsI GAGTC 4 cut(s) 416, 664, 1574, 1649
PscI ACATGT 2 cut(s) 12, 1659
PsiI TTATAA 1 cut(s) 1176
Psp6I CCWGG 3 cut(s) 98, 106, 831
PspGI CCWGG 3 cut(s) 98, 106, 831
PspN4I GGNNCC 2 cut(s) 472, 837
PsuI RGATCY 1 cut(s) 1701
PvuII CAGCTG 1 cut(s) 619
RseI CAYNNNNRTG 1 cut(s) 63
SaqAI TTAA 6 cut(s) 228, 320, 1166, 1466, 1626, 1656
SatI GCNGC 3 cut(s) 626, 876, 1035
Sau3AI GATC 8 cut(s) 40, 121, 193, 579, 760, 1516, 1564, 1701
SchI GAGTC 4 cut(s) 417, 664, 1574, 1650
ScrFI CCNGG 3 cut(s) 100, 108, 833
SduI GDGCHC 2 cut(s) 307, 1680
SfaNI GCATC 6 cut(s) 141, 631, 665, 985, 1324, 1386
SfcI CTRYAG 4 cut(s) 711, 999, 1569, 1667
SmiI ATTTAAAT 1 cut(s) 1467
SmiMI CAYNNNNRTG 1 cut(s) 63
SmlI CTYRAG 1 cut(s) 319
SmoI CTYRAG 1 cut(s) 319
SsiI CCGC 4 cut(s) 480, 817, 839, 1004
SspI AATATT 1 cut(s) 1155
SspMI CTAG 4 cut(s) 560, 891, 945, 1542
StyD4I CCNGG 3 cut(s) 98, 106, 831
SwaI ATTTAAAT 1 cut(s) 1467
TaaI ACNGT 3 cut(s) 452, 470, 1024
TaqI TCGA 4 cut(s) 137, 673, 1136, 1394
TfiI GAWTC 3 cut(s) 979, 1313, 1432
Tru1I TTAA 6 cut(s) 228, 320, 1166, 1466, 1626, 1656
Tru9I TTAA 6 cut(s) 228, 320, 1166, 1466, 1626, 1656
TscAI CASTG 3 cut(s) 457, 582, 1593
TseFI GTSAC 3 cut(s) 464, 689, 1015
TseI GCWGC 3 cut(s) 625, 875, 1034
Tsp45I GTSAC 3 cut(s) 464, 689, 1015
TspDTI ATGAA 7 cut(s) 123, 228, 528, 1023, 1145, 1305, 1445
TspGWI ACGGA 3 cut(s) 127, 647, 1221
TspRI CASTG 3 cut(s) 457, 582, 1593
Vha464I CTTAAG 1 cut(s) 319
XapI RAATTY 4 cut(s) 53, 281, 314, 1132
XceI RCATGY 2 cut(s) 16, 1663
XmiI GTMKAC 2 cut(s) 1187, 1549
XspI CTAG 4 cut(s) 560, 891, 945, 1542
Zsp2I ATGCAT 1 cut(s) 1401
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.