Rmu_sc0001639.1_g000033

PWWP domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001639.1
OG0002940
Physical Location & Seq
Reverse (-)
137569 .. 139035
1467 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1467 bp
atggaaggtaaagttgtgagtgctctgggagtggaatttactaataacagtgagtctttggattatcaagacttcaatgctagcggctgttttaagacggtaaattgtgatggagttgaaaatgaggccttggaagttaatgtggaagaagagagtaatcgaaatgaattgacgccgatgagggtccctgtgagtactgaatgcgaggttgatttgctcagtgttgctgattttatccggtattcgagaatggacgggaatgtgtcaggtggtgtgaatggaaaatcttgttcttccaagctagaatttgaagtttctgatctagtatggggcaaagtaaggactcatccatggtggcccgggcagatttgtgatccttcggctgcgtcagaactggcaaagaagtgttttaagaggggtagttatctgatagcttactttggggatcagacatttgcttggaatgaagcaccaaagattaagccctttctggagcactgctcaaaaatggagaagcagagtgatatggaagaattccaaaacgcagttggttgtgcgttggacgaagtgtcgagaagggtagagtttgggctggcctgttcttgcatatccaaacgagaatatgcaaagctcaaaactcagaagattattaatgctggaatcaggaaagaagcagcaaatagaagagatggtggtgacagttctcttagagccactttttttgaacctgcgaaagtcattaagtttataaaaaagttggctcagttgccatatggcaaagctgacagactagaacttgtaacatctctagcccagctgtcagctttcttgcgttggaagagttatgtgcctctgccagaggagaatgatactgacattctcctattgggagagaagaaccactgcaatgaagtaagtgatgaagcactctcagtacttgagaatgagaagttggtgcaaaaatcagatagtaaagagaactcattacgtgagtgcaaggacatatcagattcaaaagctcagaggtgtttgtttcctatgagtgagaatggatcagaagggaaagctggcagtgaattgattttgcaaccttgcaacaaaagaaggggtcttgatgctatagctgatgaagatgtactcccagtaatcaaagataactcaatgcataagtgtcggcacatatctgaacatagtatgaatctgagtgaagaagagcaaagcttgtcagatttggagggtacgaagtgtttgtctactccaatgagtgagaatggattagaagagaaaggtgatggtaaattgatttcacaaccttctgttaagaaacgaaaggaagttgatgctatggctgatgatgctgctgtgaaacataggaaaagtgattcatcacaaggtattggtagcaatgcgaactgtcagttgtttgaagttgaagacttttttaacctgtttttacagcagtcttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PWWP

Protein Analysis

488

Amino Acids

54.33

Weight (kDa)

5.1

Isoelectric Point (pI)

47.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PWWP PF00855 107 - 189 1.3e-18 PWWP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 749
Acc36I ACCTGC 1 cut(s) 736
AccI GTMKAC 1 cut(s) 1253
AciI CCGC 1 cut(s) 84
AclWI GGATC 3 cut(s) 368, 453, 1060
AcsI RAATTY 3 cut(s) 35, 305, 533
AcyI GRCGYC 1 cut(s) 173
AfaI GTAC 4 cut(s) 196, 936, 1137, 1240
AgsI TTSAA 7 cut(s) 76, 119, 311, 725, 1014, 1427, 1433
AhdI GACNNNNNGTC 1 cut(s) 568
AjuI GAANNNNNNNTTGG 2 cut(s) 935, 967
Alw21I GWGCWC 2 cut(s) 25, 498
AlwI GGATC 3 cut(s) 368, 453, 1060
Ama87I CYCGRG 1 cut(s) 359
AoxI GGCC 3 cut(s) 126, 356, 594
ApeKI GCWGC 3 cut(s) 383, 674, 1358
ApoI RAATTY 3 cut(s) 35, 305, 533
ArsI GACNNNNNNTTYG 2 cut(s) 1235, 1267
AseI ATTAAT 1 cut(s) 651
AspS9I GGNCC 2 cut(s) 184, 357
AsuC2I CCSGG 2 cut(s) 360, 361
AsuHPI GGTGA 2 cut(s) 707, 1301
AsuNHI GCTAGC 1 cut(s) 80
AvaI CYCGRG 1 cut(s) 359
AvaII GGWCC 1 cut(s) 184
BbsI GAAGAC 1 cut(s) 1440
Bbv12I GWGCWC 2 cut(s) 25, 498
BbvI GCAGC 3 cut(s) 370, 686, 1345
BccI CCATC 3 cut(s) 104, 683, 1286
BcgI CGANNNNNNTGC 2 cut(s) 1154, 1188
BcnI CCSGG 2 cut(s) 360, 361
BfaI CTAG 5 cut(s) 81, 302, 323, 791, 809
BfmI CTRYAG 1 cut(s) 1119
BfuAI ACCTGC 1 cut(s) 736
BisI GCNGC 4 cut(s) 85, 384, 675, 1359
BlsI GCNGC 4 cut(s) 86, 385, 676, 1360
BmcAI AGTACT 2 cut(s) 196, 936
Bme1390I CCNGG 2 cut(s) 360, 361
Bme18I GGWCC 1 cut(s) 184
BmeRI GACNNNNNGTC 1 cut(s) 568
BmeT110I CYCGRG 1 cut(s) 359
BmgT120I GGNCC 2 cut(s) 184, 357
BmiI GGNNCC 2 cut(s) 185, 186
BmrFI CCNGG 2 cut(s) 360, 361
BmrI ACTGGG 1 cut(s) 1136
BmsI GCATC 3 cut(s) 1105, 1330, 1345
BmtI GCTAGC 1 cut(s) 84
BmuI ACTGGG 1 cut(s) 1136
BpiI GAAGAC 1 cut(s) 1440
BplI GAGNNNNNCTC 2 cut(s) 485, 517
BpmI CTGGAG 1 cut(s) 512
BpuEI CTTGAG 1 cut(s) 959
BpuMI CCSGG 2 cut(s) 360, 361
BsaAI YACGTR 1 cut(s) 989
BsaHI GRCGYC 1 cut(s) 173
BsaJI CCNNGG 3 cut(s) 129, 350, 359
BsaWI WCCGGW 1 cut(s) 237
Bse1I ACTGG 2 cut(s) 399, 1142
Bse3DI GCAATG 2 cut(s) 913, 1411
BseDI CCNNGG 3 cut(s) 129, 350, 359
BseGI GGATG 1 cut(s) 346
BseMI GCAATG 2 cut(s) 913, 1411
BseMII CTCAG 6 cut(s) 232, 653, 776, 945, 1034, 1193
BseNI ACTGG 2 cut(s) 399, 1142
BseRI GAGGAG 1 cut(s) 875
BseXI GCAGC 3 cut(s) 370, 686, 1345
BseYI CCCAGC 1 cut(s) 813
BshFI GGCC 3 cut(s) 128, 358, 596
BsiHKAI GWGCWC 2 cut(s) 25, 498
BsiHKCI CYCGRG 1 cut(s) 359
BsiSI CCGG 2 cut(s) 238, 360
BslFI GGGAC 1 cut(s) 170
BsmFI GGGAC 1 cut(s) 170
BsmI GAATGC 1 cut(s) 206
BsnI GGCC 3 cut(s) 128, 358, 596
BsoBI CYCGRG 1 cut(s) 359
Bsp1286I GDGCHC 2 cut(s) 25, 498
Bsp143I GATC 4 cut(s) 319, 373, 445, 1052
Bsp19I CCATGG 1 cut(s) 350
BspACI CCGC 1 cut(s) 84
BspANI GGCC 3 cut(s) 128, 358, 596
BspCNI CTCAG 6 cut(s) 231, 652, 775, 944, 1033, 1194
BspLI GGNNCC 2 cut(s) 185, 186
BspMI ACCTGC 1 cut(s) 736
BspOI GCTAGC 1 cut(s) 84
BspPI GGATC 3 cut(s) 368, 453, 1060
BspQI GCTCTTC 1 cut(s) 1206
BsrDI GCAATG 2 cut(s) 913, 1411
BsrI ACTGG 2 cut(s) 399, 1142
BssECI CCNNGG 3 cut(s) 129, 350, 359
BssMI GATC 4 cut(s) 319, 373, 445, 1052
BssNI GRCGYC 1 cut(s) 173
BssT1I CCWWGG 2 cut(s) 129, 350
Bst4CI ACNGT 4 cut(s) 50, 100, 701, 1415
Bst6I CTCTTC 5 cut(s) 144, 679, 833, 1206, 1275
BstACI GRCGYC 1 cut(s) 173
BstBAI YACGTR 1 cut(s) 989
BstC8I GCNNGC 3 cut(s) 82, 594, 1069
BstDEI CTNAG 7 cut(s) 218, 639, 707, 762, 931, 1020, 1202
BstDSI CCRYGG 1 cut(s) 350
BstF5I GGATG 1 cut(s) 346
BstKTI GATC 4 cut(s) 322, 376, 448, 1055
BstMBI GATC 4 cut(s) 319, 373, 445, 1052
BstMWI GCNNNNNNNGC 1 cut(s) 1355
BstSCI CCNGG 2 cut(s) 358, 359
BstSFI CTRYAG 1 cut(s) 1119
BstV1I GCAGC 3 cut(s) 370, 686, 1345
BstV2I GAAGAC 1 cut(s) 1440
BsuRI GGCC 3 cut(s) 128, 358, 596
BtgI CCRYGG 1 cut(s) 350
BtsCI GGATG 1 cut(s) 346
BtsI GCAGTG 3 cut(s) 496, 901, 1078
BtsIMutI CAGTG 5 cut(s) 55, 226, 496, 901, 1078
BveI ACCTGC 1 cut(s) 736
Cac8I GCNNGC 3 cut(s) 82, 594, 1069
Cfr13I GGNCC 2 cut(s) 184, 357
Cfr9I CCCGGG 1 cut(s) 359
CseI GACGC 2 cut(s) 181, 375
Csp6I GTAC 4 cut(s) 195, 935, 1136, 1239
CspCI CAANNNNNGTGG 2 cut(s) 703, 738
CviAII CATG 1 cut(s) 351
CviQI GTAC 4 cut(s) 195, 935, 1136, 1239
DdeI CTNAG 7 cut(s) 218, 639, 707, 762, 931, 1020, 1202
DpnI GATC 4 cut(s) 321, 375, 447, 1054
DpnII GATC 4 cut(s) 319, 373, 445, 1052
DriI GACNNNNNGTC 1 cut(s) 568
Eam1104I CTCTTC 5 cut(s) 144, 679, 833, 1206, 1275
Eam1105I GACNNNNNGTC 1 cut(s) 568
EarI CTCTTC 5 cut(s) 144, 679, 833, 1206, 1275
Eco130I CCWWGG 2 cut(s) 129, 350
Eco147I AGGCCT 1 cut(s) 128
Eco47I GGWCC 1 cut(s) 184
Eco88I CYCGRG 1 cut(s) 359
EcoO109I RGGNCCY 1 cut(s) 184
EcoRI GAATTC 1 cut(s) 533
EcoT14I CCWWGG 2 cut(s) 129, 350
EcoT22I ATGCAT 1 cut(s) 1167
ErhI CCWWGG 2 cut(s) 129, 350
FaeI CATG 1 cut(s) 354
FaqI GGGAC 1 cut(s) 170
FatI CATG 1 cut(s) 350
FauNDI CATATG 1 cut(s) 772
FblI GTMKAC 1 cut(s) 1253
Fnu4HI GCNGC 4 cut(s) 85, 384, 675, 1359
FokI GGATG 1 cut(s) 333
Fsp4HI GCNGC 4 cut(s) 85, 384, 675, 1359
FspBI CTAG 5 cut(s) 81, 302, 323, 791, 809
GluI GCNGC 4 cut(s) 85, 384, 675, 1359
GsaI CCCAGC 1 cut(s) 817
GsuI CTGGAG 1 cut(s) 512
HaeIII GGCC 3 cut(s) 128, 358, 596
HapII CCGG 2 cut(s) 238, 360
HgaI GACGC 2 cut(s) 181, 375
Hin1I GRCGYC 1 cut(s) 173
Hin1II CATG 1 cut(s) 354
HindIII AAGCTT 1 cut(s) 1219
HinfI GANTC 6 cut(s) 53, 343, 660, 1010, 1198, 1382
HpaII CCGG 2 cut(s) 238, 360
HphI GGTGA 2 cut(s) 707, 1301
Hpy166II GTNNAC 1 cut(s) 1254
Hpy188III TCNNGA 6 cut(s) 68, 246, 491, 573, 664, 1112
Hpy8I GTNNAC 1 cut(s) 1254
HpyAV CCTTC 5 cut(s) 387, 570, 1052, 1098, 1323
HpyCH4III ACNGT 4 cut(s) 50, 100, 701, 1415
HpyCH4IV ACGT 1 cut(s) 988
HpyCH4V TGCA 8 cut(s) 606, 626, 906, 958, 996, 1087, 1095, 1165
HpyF10VI GCNNNNNNNGC 1 cut(s) 1355
HpyF3I CTNAG 7 cut(s) 218, 639, 707, 762, 931, 1020, 1202
HpySE526I ACGT 1 cut(s) 988
Hsp92I GRCGYC 1 cut(s) 173
Hsp92II CATG 1 cut(s) 354
KflI GGGWCCC 1 cut(s) 184
Kzo9I GATC 4 cut(s) 319, 373, 445, 1052
LguI GCTCTTC 1 cut(s) 1206
LmnI GCTCC 1 cut(s) 493
Lsp1109I GCAGC 3 cut(s) 370, 686, 1345
LweI GCATC 3 cut(s) 1105, 1330, 1345
MaeI CTAG 5 cut(s) 81, 302, 323, 791, 809
MaeII ACGT 1 cut(s) 988
MaeIII GTNAC 2 cut(s) 695, 799
MalI GATC 4 cut(s) 321, 375, 447, 1054
MboI GATC 4 cut(s) 319, 373, 445, 1052
MhlI GDGCHC 2 cut(s) 25, 498
MluCI AATT 7 cut(s) 35, 103, 167, 305, 533, 1076, 1298
MlyI GAGTC 2 cut(s) 62, 337
MmeI TCCRAC 2 cut(s) 540, 815
MnlI CCTC 8 cut(s) 118, 174, 199, 408, 853, 861, 1017, 1228
Mph1103I ATGCAT 1 cut(s) 1167
MseI TTAA 9 cut(s) 93, 138, 411, 480, 651, 741, 1320, 1443, 1465
MslI CAYNNNNRTG 1 cut(s) 906
MspA1I CMGCKG 1 cut(s) 817
MspI CCGG 2 cut(s) 238, 360
MspR9I CCNGG 2 cut(s) 360, 361
Mva1269I GAATGC 1 cut(s) 206
MwoI GCNNNNNNNGC 1 cut(s) 1355
NciI CCSGG 2 cut(s) 360, 361
NcoI CCATGG 1 cut(s) 350
NdeI CATATG 1 cut(s) 772
NdeII GATC 4 cut(s) 319, 373, 445, 1052
NheI GCTAGC 1 cut(s) 80
NlaIII CATG 1 cut(s) 354
NlaIV GGNNCC 2 cut(s) 185, 186
NmuCI GTSAC 1 cut(s) 695
NsiI ATGCAT 1 cut(s) 1167
PceI AGGCCT 1 cut(s) 128
PciSI GCTCTTC 1 cut(s) 1206
PctI GAATGC 1 cut(s) 206
PfeI GAWTC 4 cut(s) 660, 1010, 1198, 1382
PkrI GCNGC 4 cut(s) 86, 385, 676, 1360
PleI GAGTC 2 cut(s) 61, 337
PpsI GAGTC 2 cut(s) 61, 337
Ppu21I YACGTR 1 cut(s) 989
PpuMI RGGWCCY 1 cut(s) 184
PshBI ATTAAT 1 cut(s) 651
PsiI TTATAA 1 cut(s) 749
Psp5II RGGWCCY 1 cut(s) 184
PspFI CCCAGC 1 cut(s) 813
PspN4I GGNNCC 2 cut(s) 185, 186
PspPI GGNCC 2 cut(s) 184, 357
PspPPI RGGWCCY 1 cut(s) 184
PvuII CAGCTG 1 cut(s) 817
RsaI GTAC 4 cut(s) 196, 936, 1137, 1240
RsaNI GTAC 4 cut(s) 195, 935, 1136, 1239
RseI CAYNNNNRTG 1 cut(s) 906
SapI GCTCTTC 1 cut(s) 1206
SaqAI TTAA 9 cut(s) 93, 138, 411, 480, 651, 741, 1320, 1443, 1465
SatI GCNGC 4 cut(s) 85, 384, 675, 1359
Sau3AI GATC 4 cut(s) 319, 373, 445, 1052
Sau96I GGNCC 2 cut(s) 184, 357
ScaI AGTACT 2 cut(s) 196, 936
SchI GAGTC 2 cut(s) 62, 337
ScrFI CCNGG 2 cut(s) 360, 361
SduI GDGCHC 2 cut(s) 25, 498
SfaNI GCATC 3 cut(s) 1105, 1330, 1345
SfcI CTRYAG 1 cut(s) 1119
SinI GGWCC 1 cut(s) 184
SmaI CCCGGG 1 cut(s) 361
SmiMI CAYNNNNRTG 1 cut(s) 906
SmlI CTYRAG 1 cut(s) 938
SmoI CTYRAG 1 cut(s) 938
SrfI GCCCGGGC 1 cut(s) 361
Sse9I AATT 7 cut(s) 35, 103, 167, 305, 533, 1076, 1298
SseBI AGGCCT 1 cut(s) 128
SsiI CCGC 1 cut(s) 84
SspMI CTAG 5 cut(s) 81, 302, 323, 791, 809
StuI AGGCCT 1 cut(s) 128
StyD4I CCNGG 2 cut(s) 358, 359
StyI CCWWGG 2 cut(s) 129, 350
TaaI ACNGT 4 cut(s) 50, 100, 701, 1415
TaiI ACGT 1 cut(s) 991
TaqI TCGA 3 cut(s) 160, 245, 572
TasI AATT 7 cut(s) 35, 103, 167, 305, 533, 1076, 1298
TatI WGTACW 3 cut(s) 194, 934, 1135
TauI GCSGC 1 cut(s) 87
TfiI GAWTC 4 cut(s) 660, 1010, 1198, 1382
Tru1I TTAA 9 cut(s) 93, 138, 411, 480, 651, 741, 1320, 1443, 1465
Tru9I TTAA 9 cut(s) 93, 138, 411, 480, 651, 741, 1320, 1443, 1465
TscAI CASTG 5 cut(s) 55, 226, 503, 908, 1078
TseFI GTSAC 1 cut(s) 695
TseI GCWGC 3 cut(s) 383, 674, 1358
Tsp45I GTSAC 1 cut(s) 695
TspDTI ATGAA 7 cut(s) 180, 480, 924, 936, 1143, 1211, 1374
TspMI CCCGGG 1 cut(s) 359
TspRI CASTG 5 cut(s) 55, 226, 503, 908, 1078
VpaK11BI GGWCC 1 cut(s) 184
VspI ATTAAT 1 cut(s) 651
XapI RAATTY 3 cut(s) 35, 305, 533
XcmI CCANNNNNNNNNTGG 1 cut(s) 545
XmaI CCCGGG 1 cut(s) 359
XmiI GTMKAC 1 cut(s) 1253
XspI CTAG 5 cut(s) 81, 302, 323, 791, 809
ZrmI AGTACT 2 cut(s) 196, 936
Zsp2I ATGCAT 1 cut(s) 1167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.