Rmu_sc0001889.1_g000008

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001889.1
OG0001843
Physical Location & Seq
Reverse (-)
30997 .. 35437
4441 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1398 bp
atggaggtggtgaccgggaactcattgtttgggaaagtaggaacggaagggatagcggaagcggcgggggttggtttggcttcagtgacatgttcagtggttgctgcttggatatccagtgctcgaatccgagtagggatttgggggattagaaatgaggagttgagtggcagtggcttgaaaaagatgaataggcatggttgtggtggagaggtcggtggtggttatgggtgtagtgctagatctgagttgagttttctgttttctgttagtgcaatggcttgtgggatgaagccttgggtggtggtgattgctctgacttggtttttaagcctgcaagaacattcttcttctgttcatggaaagccacaagtaccttgtttcttcatttttggagactcactggctgataatggcaacgacaacgtgcttgatactttggctaaagtcaattaccagccctatggtatcgacttccctagtggcacagcaaccggaagattcagcaacggtcgaaccaccgtcgatgttctctctgaacttttaggctttgacaactatattccaccatttgcttttgttaatggctctgacatacttaaaggtgtgaactatgcttgtggcgcggcaggaattcgaaaagaaactggcaagcaattgggtgcaagaatcagcatggccagacagttaaagaaccacaagattataatctcacgcattgtcgacatacttgggaagaagagcttagcccaaaagcacttaaacaagtgcttatattcagttggaatgggcagcaatgactacatcaacaattacttcgtgcctcagtattaccacaccagcaaaaaatttaccactgagcaatatgctgaagttctcatcaaccaatattcttgggaaatcctgaagttgtacaagtatggagcaaggaaggtggctttggttggattgggactaataggctgcaccccaagtgcaatttcttcctttggcacaaatgggtcagcatgtgtagacacattgaacattgcagcgcaactgtttaaccaaaggcttgtatcacttgtggacaagctcaacagcaatttgacagatgcaaaattcatctatattaacagctttgaaatgggttctggagatcctgcagctgctggcttcacagtttcgaatgttgggtgttgtgcggtaaatgaagttggtcaatgtaatgttgaccatactccatgcaagaacaggactgagtacgtgttctgggacggattccatcctactgaagccttgaaccgaatcactgcaatcagatcatacaatgcttttaaccctgtagacacttatccaatggatattagtcaccttgttcagctacaaattaataactcagcaactccagctacctag

Protein Analysis

465

Amino Acids

50.18

Weight (kDa)

8.32

Isoelectric Point (pI)

20.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 129 - 432 3.6e-33 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 707
AccI GTMKAC 3 cut(s) 723, 1014, 1326
AccII CGCG 1 cut(s) 626
AciI CCGC 5 cut(s) 56, 62, 65, 626, 1184
AclWI GGATC 1 cut(s) 1133
AcoI YGGCCR 1 cut(s) 678
AcsI RAATTY 3 cut(s) 633, 848, 1100
AcuI CTGAAG 4 cut(s) 66, 891, 926, 1293
AfaI GTAC 3 cut(s) 375, 914, 1244
AflIII ACRYGT 2 cut(s) 89, 1245
AgsI TTSAA 4 cut(s) 181, 1024, 1124, 1282
AjuI GAANNNNNNNTTGG 4 cut(s) 923, 955, 964, 996
AluBI AGCT 6 cut(s) 744, 1075, 1119, 1148, 1363, 1391
AluI AGCT 6 cut(s) 744, 1075, 1119, 1148, 1363, 1391
Alw21I GWGCWC 1 cut(s) 124
Alw26I GTCTC 1 cut(s) 390
AlwI GGATC 1 cut(s) 1133
AlwNI CAGNNNCTG 1 cut(s) 1151
AoxI GGCC 1 cut(s) 678
ApeKI GCWGC 6 cut(s) 104, 792, 963, 1031, 1145, 1148
ApoI RAATTY 3 cut(s) 633, 848, 1100
AseI ATTAAT 1 cut(s) 1371
AspLEI GCGC 2 cut(s) 626, 1036
AsuC2I CCSGG 1 cut(s) 16
AsuHPI GGTGA 3 cut(s) 22, 319, 1343
AsuII TTCGAA 2 cut(s) 637, 1166
BalI TGGCCA 1 cut(s) 680
Bbv12I GWGCWC 1 cut(s) 124
BbvI GCAGC 6 cut(s) 91, 804, 950, 1043, 1135, 1157
BccI CCATC 1 cut(s) 1272
BcnI CCSGG 1 cut(s) 16
BcoDI GTCTC 1 cut(s) 390
BfaI CTAG 3 cut(s) 240, 480, 1396
BfmI CTRYAG 2 cut(s) 1143, 1323
BglII AGATCT 1 cut(s) 242
BisI GCNGC 8 cut(s) 63, 105, 627, 793, 964, 1032, 1146, 1149
BlpI GCTNAGC 1 cut(s) 745
BlsI GCNGC 8 cut(s) 64, 106, 628, 794, 965, 1033, 1147, 1150
Bme1390I CCNGG 1 cut(s) 16
BmrFI CCNGG 1 cut(s) 16
BmsI GCATC 1 cut(s) 1084
BpmI CTGGAG 2 cut(s) 1155, 1371
Bpu1102I GCTNAGC 1 cut(s) 745
Bpu14I TTCGAA 2 cut(s) 637, 1166
BpuMI CCSGG 1 cut(s) 16
BsaAI YACGTR 1 cut(s) 1246
BsaBI GATNNNNATC 1 cut(s) 707
BsaJI CCNNGG 1 cut(s) 296
BsaWI WCCGGW 1 cut(s) 494
Bse1I ACTGG 3 cut(s) 117, 408, 652
Bse3DI GCAATG 3 cut(s) 282, 802, 1026
Bse8I GATNNNNATC 1 cut(s) 707
BseDI CCNNGG 1 cut(s) 296
BseGI GGATG 2 cut(s) 294, 1264
BseJI GATNNNNATC 1 cut(s) 707
BseMI GCAATG 3 cut(s) 282, 802, 1026
BseMII CTCAG 5 cut(s) 237, 839, 849, 1230, 1392
BseNI ACTGG 3 cut(s) 117, 408, 652
BseRI GAGGAG 1 cut(s) 173
BseXI GCAGC 6 cut(s) 91, 804, 950, 1043, 1135, 1157
BsgI GTGCAG 1 cut(s) 949
Bsh1236I CGCG 1 cut(s) 626
Bsh1285I CGRYCG 1 cut(s) 514
BshFI GGCC 1 cut(s) 680
BsiEI CGRYCG 1 cut(s) 514
BsiHKAI GWGCWC 1 cut(s) 124
BsiSI CCGG 2 cut(s) 15, 495
BslFI GGGAC 2 cut(s) 966, 1268
BsmAI GTCTC 1 cut(s) 390
BsmFI GGGAC 2 cut(s) 966, 1268
BsnI GGCC 1 cut(s) 680
Bsp119I TTCGAA 2 cut(s) 637, 1166
Bsp1286I GDGCHC 1 cut(s) 124
Bsp1407I TGTACA 1 cut(s) 912
Bsp143I GATC 3 cut(s) 242, 1138, 1301
Bsp1720I GCTNAGC 1 cut(s) 745
BspACI CCGC 5 cut(s) 56, 62, 65, 626, 1184
BspANI GGCC 1 cut(s) 680
BspCNI CTCAG 5 cut(s) 238, 838, 850, 1231, 1391
BspFNI CGCG 1 cut(s) 626
BspMAI CTGCAG 1 cut(s) 1147
BspPI GGATC 1 cut(s) 1133
BspQI GCTCTTC 1 cut(s) 734
BspT104I TTCGAA 2 cut(s) 637, 1166
BsrDI GCAATG 3 cut(s) 282, 802, 1026
BsrGI TGTACA 1 cut(s) 912
BsrI ACTGG 3 cut(s) 117, 408, 652
BssECI CCNNGG 1 cut(s) 296
BssMI GATC 3 cut(s) 242, 1138, 1301
BssT1I CCWWGG 1 cut(s) 296
Bst4CI ACNGT 5 cut(s) 512, 523, 687, 1041, 1162
Bst6I CTCTTC 1 cut(s) 734
BstAUI TGTACA 1 cut(s) 912
BstBAI YACGTR 1 cut(s) 1246
BstBI TTCGAA 2 cut(s) 637, 1166
BstC8I GCNNGC 3 cut(s) 335, 653, 1153
BstDEI CTNAG 6 cut(s) 246, 745, 825, 858, 1239, 1378
BstEII GGTNACC 1 cut(s) 10
BstF5I GGATG 2 cut(s) 294, 1264
BstFNI CGCG 1 cut(s) 626
BstHHI GCGC 2 cut(s) 626, 1036
BstKTI GATC 3 cut(s) 245, 1141, 1304
BstMAI GTCTC 1 cut(s) 390
BstMBI GATC 3 cut(s) 242, 1138, 1301
BstMCI CGRYCG 1 cut(s) 514
BstMWI GCNNNNNNNGC 3 cut(s) 62, 623, 1388
BstNSI RCATGY 2 cut(s) 93, 1011
BstPI GGTNACC 1 cut(s) 10
BstSCI CCNGG 1 cut(s) 14
BstSFI CTRYAG 2 cut(s) 1143, 1323
BstUI CGCG 1 cut(s) 626
BstV1I GCAGC 6 cut(s) 91, 804, 950, 1043, 1135, 1157
BstX2I RGATCY 2 cut(s) 242, 1138
BstXI CCANNNNNNTGG 1 cut(s) 464
BstYI RGATCY 2 cut(s) 242, 1138
BsuRI GGCC 1 cut(s) 680
BtsCI GGATG 2 cut(s) 294, 1264
BtsI GCAGTG 2 cut(s) 178, 1290
BtsIMutI CAGTG 7 cut(s) 90, 102, 124, 178, 401, 855, 1290
Cac8I GCNNGC 3 cut(s) 335, 653, 1153
CaiI CAGNNNCTG 1 cut(s) 1151
CfoI GCGC 2 cut(s) 626, 1036
Csp6I GTAC 3 cut(s) 374, 913, 1243
CspCI CAANNNNNGTGG 2 cut(s) 915, 950
CviAII CATG 6 cut(s) 90, 197, 359, 676, 1008, 1224
CviQI GTAC 3 cut(s) 374, 913, 1243
DdeI CTNAG 6 cut(s) 246, 745, 825, 858, 1239, 1378
DpnI GATC 3 cut(s) 244, 1140, 1303
DpnII GATC 3 cut(s) 242, 1138, 1301
EaeI YGGCCR 1 cut(s) 678
Eam1104I CTCTTC 1 cut(s) 734
EarI CTCTTC 1 cut(s) 734
Eco130I CCWWGG 1 cut(s) 296
Eco32I GATATC 1 cut(s) 114
Eco57I CTGAAG 4 cut(s) 66, 891, 926, 1293
Eco91I GGTNACC 1 cut(s) 10
EcoO65I GGTNACC 1 cut(s) 10
EcoRI GAATTC 1 cut(s) 633
EcoRV GATATC 1 cut(s) 114
EcoT14I CCWWGG 1 cut(s) 296
ErhI CCWWGG 1 cut(s) 296
FaeI CATG 6 cut(s) 93, 200, 362, 679, 1011, 1227
FalI AAGNNNNNCTT 2 cut(s) 728, 760
FaqI GGGAC 2 cut(s) 966, 1268
FatI CATG 6 cut(s) 89, 196, 358, 675, 1007, 1223
FauI CCCGC 1 cut(s) 58
FblI GTMKAC 3 cut(s) 723, 1014, 1326
Fnu4HI GCNGC 8 cut(s) 63, 105, 627, 793, 964, 1032, 1146, 1149
FokI GGATG 2 cut(s) 301, 1251
Fsp4HI GCNGC 8 cut(s) 63, 105, 627, 793, 964, 1032, 1146, 1149
FspBI CTAG 3 cut(s) 240, 480, 1396
GlaI GCGC 2 cut(s) 625, 1035
GluI GCNGC 8 cut(s) 63, 105, 627, 793, 964, 1032, 1146, 1149
GsuI CTGGAG 2 cut(s) 1155, 1371
HaeIII GGCC 1 cut(s) 680
HapII CCGG 2 cut(s) 15, 495
HhaI GCGC 2 cut(s) 626, 1036
Hin1II CATG 6 cut(s) 93, 200, 362, 679, 1011, 1227
Hin6I GCGC 2 cut(s) 624, 1034
HinP1I GCGC 2 cut(s) 624, 1034
HincII GTYRAC 2 cut(s) 724, 1213
HindII GTYRAC 2 cut(s) 724, 1213
HinfI GANTC 6 cut(s) 126, 398, 501, 669, 1260, 1287
HpaII CCGG 2 cut(s) 15, 495
HphI GGTGA 3 cut(s) 22, 319, 1343
Hpy166II GTNNAC 6 cut(s) 610, 724, 1015, 1069, 1213, 1327
Hpy188I TCNGA 6 cut(s) 131, 247, 318, 538, 592, 1301
Hpy188III TCNNGA 2 cut(s) 904, 1134
Hpy8I GTNNAC 6 cut(s) 610, 724, 1015, 1069, 1213, 1327
Hpy99I CGWCG 1 cut(s) 527
HpyAV CCTTC 2 cut(s) 41, 925
HpyCH4III ACNGT 5 cut(s) 512, 523, 687, 1041, 1162
HpyCH4IV ACGT 2 cut(s) 426, 1245
HpyF10VI GCNNNNNNNGC 3 cut(s) 62, 623, 1388
HpyF3I CTNAG 6 cut(s) 246, 745, 825, 858, 1239, 1378
HpySE526I ACGT 2 cut(s) 426, 1245
Hsp92II CATG 6 cut(s) 93, 200, 362, 679, 1011, 1227
HspAI GCGC 2 cut(s) 624, 1034
Kzo9I GATC 3 cut(s) 242, 1138, 1301
LguI GCTCTTC 1 cut(s) 734
LmnI GCTCC 1 cut(s) 923
Lsp1109I GCAGC 6 cut(s) 91, 804, 950, 1043, 1135, 1157
LweI GCATC 1 cut(s) 1084
MaeI CTAG 3 cut(s) 240, 480, 1396
MaeII ACGT 2 cut(s) 426, 1245
MaeIII GTNAC 3 cut(s) 10, 85, 1349
MalI GATC 3 cut(s) 244, 1140, 1303
MboI GATC 3 cut(s) 242, 1138, 1301
MboII GAAGA 7 cut(s) 339, 342, 376, 510, 748, 751, 975
MfeI CAATTG 1 cut(s) 656
MflI RGATCY 2 cut(s) 242, 1138
MhlI GDGCHC 1 cut(s) 124
MlsI TGGCCA 1 cut(s) 680
MluCI AATT 9 cut(s) 451, 633, 656, 811, 848, 978, 1084, 1100, 1368
MluNI TGGCCA 1 cut(s) 680
MlyI GAGTC 1 cut(s) 392
MmeI TCCRAC 2 cut(s) 763, 925
MnlI CCTC 3 cut(s) 151, 205, 834
Mox20I TGGCCA 1 cut(s) 680
MscI TGGCCA 1 cut(s) 680
MseI TTAA 9 cut(s) 329, 582, 600, 689, 761, 1044, 1113, 1317, 1371
MslI CAYNNNNRTG 1 cut(s) 201
Msp20I TGGCCA 1 cut(s) 680
MspA1I CMGCKG 1 cut(s) 1148
MspI CCGG 2 cut(s) 15, 495
MspR9I CCNGG 1 cut(s) 16
MunI CAATTG 1 cut(s) 656
MvnI CGCG 1 cut(s) 626
MwoI GCNNNNNNNGC 3 cut(s) 62, 623, 1388
NciI CCSGG 1 cut(s) 16
NdeII GATC 3 cut(s) 242, 1138, 1301
NlaIII CATG 6 cut(s) 93, 200, 362, 679, 1011, 1227
NmuCI GTSAC 3 cut(s) 10, 85, 1349
NspI RCATGY 2 cut(s) 93, 1011
NspV TTCGAA 2 cut(s) 637, 1166
PciI ACATGT 1 cut(s) 89
PciSI GCTCTTC 1 cut(s) 734
PfeI GAWTC 5 cut(s) 126, 501, 669, 1260, 1287
PkrI GCNGC 8 cut(s) 64, 106, 628, 794, 965, 1033, 1147, 1150
PleI GAGTC 1 cut(s) 392
PpsI GAGTC 1 cut(s) 392
Ppu21I YACGTR 1 cut(s) 1246
PscI ACATGT 1 cut(s) 89
PshBI ATTAAT 1 cut(s) 1371
PsiI TTATAA 1 cut(s) 707
PspEI GGTNACC 1 cut(s) 10
PstI CTGCAG 1 cut(s) 1147
PstNI CAGNNNCTG 1 cut(s) 1151
PsuI RGATCY 2 cut(s) 242, 1138
PvuII CAGCTG 1 cut(s) 1148
RsaI GTAC 3 cut(s) 375, 914, 1244
RsaNI GTAC 3 cut(s) 374, 913, 1243
RseI CAYNNNNRTG 1 cut(s) 201
SalI GTCGAC 1 cut(s) 722
SapI GCTCTTC 1 cut(s) 734
SaqAI TTAA 9 cut(s) 329, 582, 600, 689, 761, 1044, 1113, 1317, 1371
SatI GCNGC 8 cut(s) 63, 105, 627, 793, 964, 1032, 1146, 1149
Sau3AI GATC 3 cut(s) 242, 1138, 1301
SchI GAGTC 1 cut(s) 392
ScrFI CCNGG 1 cut(s) 16
SduI GDGCHC 1 cut(s) 124
SfaNI GCATC 1 cut(s) 1084
SfcI CTRYAG 2 cut(s) 1143, 1323
SfuI TTCGAA 2 cut(s) 637, 1166
SmiMI CAYNNNNRTG 1 cut(s) 201
Sse9I AATT 9 cut(s) 451, 633, 656, 811, 848, 978, 1084, 1100, 1368
SsiI CCGC 5 cut(s) 56, 62, 65, 626, 1184
SspI AATATT 1 cut(s) 890
SspMI CTAG 3 cut(s) 240, 480, 1396
StyD4I CCNGG 1 cut(s) 14
StyI CCWWGG 1 cut(s) 296
TaaI ACNGT 5 cut(s) 512, 523, 687, 1041, 1162
TaiI ACGT 2 cut(s) 429, 1248
TaqI TCGA 7 cut(s) 124, 471, 514, 525, 637, 723, 1166
TasI AATT 9 cut(s) 451, 633, 656, 811, 848, 978, 1084, 1100, 1368
TatI WGTACW 1 cut(s) 912
TauI GCSGC 2 cut(s) 65, 629
TfiI GAWTC 5 cut(s) 126, 501, 669, 1260, 1287
Tru1I TTAA 9 cut(s) 329, 582, 600, 689, 761, 1044, 1113, 1317, 1371
Tru9I TTAA 9 cut(s) 329, 582, 600, 689, 761, 1044, 1113, 1317, 1371
TscAI CASTG 7 cut(s) 90, 102, 124, 178, 408, 862, 1297
TseFI GTSAC 3 cut(s) 10, 85, 1349
TseI GCWGC 6 cut(s) 104, 792, 963, 1031, 1145, 1148
Tsp45I GTSAC 3 cut(s) 10, 85, 1349
TspDTI ATGAA 6 cut(s) 203, 305, 347, 376, 1093, 1206
TspGWI ACGGA 2 cut(s) 59, 1272
TspRI CASTG 7 cut(s) 90, 102, 124, 178, 408, 862, 1297
VspI ATTAAT 1 cut(s) 1371
XapI RAATTY 3 cut(s) 633, 848, 1100
XceI RCATGY 2 cut(s) 93, 1011
XmiI GTMKAC 3 cut(s) 723, 1014, 1326
XspI CTAG 3 cut(s) 240, 480, 1396
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.