Rmu_sc0002012.1_g000015

isoflavone

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002012.1
OG0003183
Physical Location & Seq
Forward (+)
87255 .. 88830
1576 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 858 bp
atgggcgagcagcttttgaacggtgataaggctctctgtgtcggtgaaggatcggcctcggccgtgatggcgttgcaagatttggggtttcccaatgctcgtgaaatggagaagaagaagagtagagtactgataatcagagcaacaggaaatctgggtcaccatttagccaatgccagcctccatttctctcaccccactttcgctctcgtccgacccgacaccttctccgacccccacaagtcccaaaaccttcgagccctctccgatgccggcgtcacactcctccaagtcggttcgctggaagacgaagagaaccttgttggagcagtgaagcaagttgatgttgtgatttgtgctgttcaggtcaaacagagattcattccatcagaattcgggttggacccagataagacgcaaatttcagggatggactacaacttctattcaaacaaagccgagattaggcgtcttgtggaagctcaaggcattccctacacttttatttcctgcaatttctatacgagctatttgcttccttcacttgtgcagccgggcctcaaagtgcctccaagggacaaagtcacgattttcggggacggaaatactaaaggcgtttttgtcaaggaaattgatgtagctgccttcactatcaggacattggacgatccacgaaccttgaacaaagtgttgtattttaggccacccggaaatgtgtactcgctgaatgaacttgttgagctttgggagagtaagattgggaagaagcttgagaaggtgtttgtatcagaagaagagcttcttaagaagagtaaaggtacttcttctagttcatactatttgggtatttcacaatga

Protein Analysis

285

Amino Acids

31.5

Weight (kDa)

6.97

Isoelectric Point (pI)

29.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 39 - 270 8.8e-51 NmrA-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 58, 662
AcoI YGGCCR 1 cut(s) 60
AcsI RAATTY 2 cut(s) 392, 420
AcyI GRCGYC 2 cut(s) 276, 469
AfaI GTAC 3 cut(s) 129, 719, 820
AfiI CCNNNNNNNGG 1 cut(s) 465
AflII CTTAAG 1 cut(s) 803
AgsI TTSAA 3 cut(s) 19, 450, 682
AluBI AGCT 7 cut(s) 13, 482, 528, 641, 742, 769, 799
AluI AGCT 7 cut(s) 13, 482, 528, 641, 742, 769, 799
AlwI GGATC 2 cut(s) 58, 662
AoxI GGCC 4 cut(s) 54, 60, 556, 701
ApeKI GCWGC 3 cut(s) 10, 550, 641
ApoI RAATTY 2 cut(s) 392, 420
Asp700I GAANNNNTTC 1 cut(s) 798
AspS9I GGNCC 2 cut(s) 403, 556
AsuC2I CCSGG 2 cut(s) 555, 708
AsuHPI GGTGA 4 cut(s) 35, 56, 152, 185
AvaII GGWCC 1 cut(s) 403
BanII GRGCYC 1 cut(s) 262
BarI GAAGNNNNNNTAC 2 cut(s) 805, 837
BauI CACGAG 1 cut(s) 99
BbsI GAAGAC 1 cut(s) 312
BbvI GCAGC 3 cut(s) 22, 562, 628
BccI CCATC 3 cut(s) 61, 394, 424
BceAI ACGGC 1 cut(s) 47
BcgI CGANNNNNNTGC 2 cut(s) 514, 548
BcnI CCSGG 2 cut(s) 555, 708
BfaI CTAG 1 cut(s) 828
BfrI CTTAAG 1 cut(s) 803
BglI GCCNNNNNGGC 1 cut(s) 68
BisI GCNGC 3 cut(s) 11, 551, 642
BlsI GCNGC 3 cut(s) 12, 552, 643
BmcAI AGTACT 1 cut(s) 129
Bme1390I CCNGG 2 cut(s) 555, 708
Bme18I GGWCC 1 cut(s) 403
BmgT120I GGNCC 2 cut(s) 403, 556
BmiI GGNNCC 1 cut(s) 405
BmrFI CCNGG 2 cut(s) 555, 708
BmsI GCATC 1 cut(s) 259
BpiI GAAGAC 1 cut(s) 312
BpuEI CTTGAG 2 cut(s) 468, 791
BpuMI CCSGG 2 cut(s) 555, 708
BsaHI GRCGYC 2 cut(s) 276, 469
BsaJI CCNNGG 2 cut(s) 57, 572
BsaXI ACNNNNNCTCC 2 cut(s) 212, 242
Bsc4I CCNNNNNNNGG 1 cut(s) 465
Bse118I RCCGGY 1 cut(s) 272
BseDI CCNNGG 2 cut(s) 57, 572
BseGI GGATG 1 cut(s) 435
BseLI CCNNNNNNNGG 1 cut(s) 465
BseRI GAGGAG 1 cut(s) 275
BseX3I CGGCCG 1 cut(s) 60
BseXI GCAGC 3 cut(s) 22, 562, 628
BsgI GTGCAG 1 cut(s) 569
Bsh1285I CGRYCG 1 cut(s) 63
BshFI GGCC 4 cut(s) 56, 62, 558, 703
BsiEI CGRYCG 1 cut(s) 63
BsiSI CCGG 3 cut(s) 273, 554, 708
BslFI GGGAC 3 cut(s) 229, 590, 611
BslI CCNNNNNNNGG 1 cut(s) 465
BsmFI GGGAC 3 cut(s) 229, 590, 611
BsmI GAATGC 1 cut(s) 489
BsnI GGCC 4 cut(s) 56, 62, 558, 703
Bsp1286I GDGCHC 1 cut(s) 262
Bsp143I GATC 2 cut(s) 50, 667
BspANI GGCC 4 cut(s) 56, 62, 558, 703
BspLI GGNNCC 1 cut(s) 405
BspPI GGATC 2 cut(s) 58, 662
BspQI GCTCTTC 1 cut(s) 789
BspTI CTTAAG 1 cut(s) 803
BsrFI RCCGGY 1 cut(s) 272
BssAI RCCGGY 1 cut(s) 272
BssECI CCNNGG 2 cut(s) 57, 572
BssMI GATC 2 cut(s) 50, 667
BssNI GRCGYC 2 cut(s) 276, 469
BssSI CACGAG 1 cut(s) 99
BssT1I CCWWGG 1 cut(s) 572
Bst2BI CACGAG 1 cut(s) 99
Bst4CI ACNGT 1 cut(s) 23
Bst6I CTCTTC 4 cut(s) 113, 306, 789, 803
BstACI GRCGYC 2 cut(s) 276, 469
BstAFI CTTAAG 1 cut(s) 803
BstC8I GCNNGC 3 cut(s) 8, 178, 274
BstEII GGTNACC 1 cut(s) 158
BstF5I GGATG 1 cut(s) 435
BstKTI GATC 2 cut(s) 53, 670
BstMBI GATC 2 cut(s) 50, 667
BstMCI CGRYCG 1 cut(s) 63
BstMWI GCNNNNNNNGC 1 cut(s) 68
BstPI GGTNACC 1 cut(s) 158
BstSCI CCNGG 2 cut(s) 553, 706
BstV1I GCAGC 3 cut(s) 22, 562, 628
BstV2I GAAGAC 1 cut(s) 312
BstZI CGGCCG 1 cut(s) 60
BsuRI GGCC 4 cut(s) 56, 62, 558, 703
BtsCI GGATG 1 cut(s) 435
BtsI GCAGTG 1 cut(s) 336
BtsIMutI CAGTG 1 cut(s) 336
Cac8I GCNNGC 3 cut(s) 8, 178, 274
Cfr10I RCCGGY 1 cut(s) 272
Cfr13I GGNCC 2 cut(s) 403, 556
CseI GACGC 3 cut(s) 265, 424, 458
Csp6I GTAC 3 cut(s) 128, 718, 819
CspCI CAANNNNNGTGG 2 cut(s) 660, 695
CviQI GTAC 3 cut(s) 128, 718, 819
DpnI GATC 2 cut(s) 52, 669
DpnII GATC 2 cut(s) 50, 667
EaeI YGGCCR 1 cut(s) 60
EagI CGGCCG 1 cut(s) 60
Eam1104I CTCTTC 4 cut(s) 113, 306, 789, 803
EarI CTCTTC 4 cut(s) 113, 306, 789, 803
EclXI CGGCCG 1 cut(s) 60
Eco130I CCWWGG 1 cut(s) 572
Eco24I GRGCYC 1 cut(s) 262
Eco47I GGWCC 1 cut(s) 403
Eco52I CGGCCG 1 cut(s) 60
Eco91I GGTNACC 1 cut(s) 158
EcoO65I GGTNACC 1 cut(s) 158
EcoRI GAATTC 1 cut(s) 392
EcoT14I CCWWGG 1 cut(s) 572
EcoT38I GRGCYC 1 cut(s) 262
ErhI CCWWGG 1 cut(s) 572
FaiI YATR 2 cut(s) 522, 835
FalI AAGNNNNNCTT 6 cut(s) 303, 335, 783, 815, 786, 818
FaqI GGGAC 3 cut(s) 229, 590, 611
Fnu4HI GCNGC 3 cut(s) 11, 551, 642
FokI GGATG 1 cut(s) 442
FriOI GRGCYC 1 cut(s) 262
Fsp4HI GCNGC 3 cut(s) 11, 551, 642
FspBI CTAG 1 cut(s) 828
GluI GCNGC 3 cut(s) 11, 551, 642
HaeIII GGCC 4 cut(s) 56, 62, 558, 703
HapII CCGG 3 cut(s) 273, 554, 708
HgaI GACGC 3 cut(s) 265, 424, 458
Hin1I GRCGYC 2 cut(s) 276, 469
HindIII AAGCTT 1 cut(s) 767
HinfI GANTC 1 cut(s) 378
HpaII CCGG 3 cut(s) 273, 554, 708
HphI GGTGA 4 cut(s) 35, 56, 152, 185
Hpy166II GTNNAC 1 cut(s) 718
Hpy188I TCNGA 6 cut(s) 140, 215, 232, 268, 391, 790
Hpy188III TCNNGA 3 cut(s) 101, 586, 655
Hpy8I GTNNAC 1 cut(s) 718
HpyAV CCTTC 6 cut(s) 41, 235, 263, 549, 655, 769
HpyCH4III ACNGT 1 cut(s) 23
HpyCH4V TGCA 3 cut(s) 76, 513, 550
HpyF10VI GCNNNNNNNGC 1 cut(s) 68
Hsp92I GRCGYC 2 cut(s) 276, 469
KroI GCCGGC 1 cut(s) 272
KroNI GCCGGC 1 cut(s) 274
Kzo9I GATC 2 cut(s) 50, 667
LguI GCTCTTC 1 cut(s) 789
LmnI GCTCC 1 cut(s) 326
Lsp1109I GCAGC 3 cut(s) 22, 562, 628
LweI GCATC 1 cut(s) 259
MaeI CTAG 1 cut(s) 828
MaeIII GTNAC 3 cut(s) 158, 277, 583
MalI GATC 2 cut(s) 52, 669
MboI GATC 2 cut(s) 50, 667
MhlI GDGCHC 1 cut(s) 262
MluCI AATT 4 cut(s) 392, 420, 514, 630
MmeI TCCRAC 4 cut(s) 238, 255, 304, 381
MnlI CCTC 6 cut(s) 67, 191, 272, 296, 569, 579
MroNI GCCGGC 1 cut(s) 272
MroXI GAANNNNTTC 1 cut(s) 798
MseI TTAA 1 cut(s) 804
MspCI CTTAAG 1 cut(s) 803
MspI CCGG 3 cut(s) 273, 554, 708
MspR9I CCNGG 2 cut(s) 555, 708
Mva1269I GAATGC 1 cut(s) 489
MwoI GCNNNNNNNGC 1 cut(s) 68
NaeI GCCGGC 1 cut(s) 274
NciI CCSGG 2 cut(s) 555, 708
NdeII GATC 2 cut(s) 50, 667
NgoMIV GCCGGC 1 cut(s) 272
NlaIV GGNNCC 1 cut(s) 405
NmeAIII GCCGAG 2 cut(s) 38, 484
NmuCI GTSAC 3 cut(s) 158, 277, 583
PciSI GCTCTTC 1 cut(s) 789
PcsI WCGNNNNNNNCGW 1 cut(s) 216
PctI GAATGC 1 cut(s) 489
PdiI GCCGGC 1 cut(s) 274
PdmI GAANNNNTTC 1 cut(s) 798
PfeI GAWTC 1 cut(s) 378
PflFI GACNNNGTC 1 cut(s) 581
PkrI GCNGC 3 cut(s) 12, 552, 643
PspEI GGTNACC 1 cut(s) 158
PspN4I GGNNCC 1 cut(s) 405
PspPI GGNCC 2 cut(s) 403, 556
PsyI GACNNNGTC 1 cut(s) 581
RsaI GTAC 3 cut(s) 129, 719, 820
RsaNI GTAC 3 cut(s) 128, 718, 819
SapI GCTCTTC 1 cut(s) 789
SaqAI TTAA 1 cut(s) 804
SatI GCNGC 3 cut(s) 11, 551, 642
Sau3AI GATC 2 cut(s) 50, 667
Sau96I GGNCC 2 cut(s) 403, 556
ScaI AGTACT 1 cut(s) 129
ScrFI CCNGG 2 cut(s) 555, 708
SduI GDGCHC 1 cut(s) 262
SfaNI GCATC 1 cut(s) 259
SinI GGWCC 1 cut(s) 403
SmlI CTYRAG 3 cut(s) 483, 770, 803
SmoI CTYRAG 3 cut(s) 483, 770, 803
Sse9I AATT 4 cut(s) 392, 420, 514, 630
SspMI CTAG 1 cut(s) 828
StyD4I CCNGG 2 cut(s) 553, 706
StyI CCWWGG 1 cut(s) 572
TaaI ACNGT 1 cut(s) 23
TaqI TCGA 1 cut(s) 256
TasI AATT 4 cut(s) 392, 420, 514, 630
TatI WGTACW 2 cut(s) 127, 717
TfiI GAWTC 1 cut(s) 378
Tru1I TTAA 1 cut(s) 804
Tru9I TTAA 1 cut(s) 804
TscAI CASTG 1 cut(s) 336
TseFI GTSAC 3 cut(s) 158, 277, 583
TseI GCWGC 3 cut(s) 10, 550, 641
Tsp45I GTSAC 3 cut(s) 158, 277, 583
TspDTI ATGAA 3 cut(s) 370, 744, 822
TspGWI ACGGA 1 cut(s) 615
TspRI CASTG 1 cut(s) 336
Tth111I GACNNNGTC 1 cut(s) 581
Vha464I CTTAAG 1 cut(s) 803
VpaK11BI GGWCC 1 cut(s) 403
XapI RAATTY 2 cut(s) 392, 420
XmnI GAANNNNTTC 1 cut(s) 798
XspI CTAG 1 cut(s) 828
ZrmI AGTACT 1 cut(s) 129
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.