Rmu_sc0003679.1_g000003

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003679.1
OG0004849
Physical Location & Seq
Forward (+)
8813 .. 10880
2068 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003679.1_g000003.1.cds

Sequence Viewer

Length: 591 bp
atgggcaatgcttccgtcactagcgatactgctattgatgacgagttcgttcctctccccatcccagtgggtgcgagcgttgaggaacagctcgctatcctgaagatcaacagagataaatacgaggaaaatttggccaagtcgatcgcacagtcagagaaaaatcttcgcgatctggaccatccagaaactgcgcgtgaagatggtatttctgggattgaattgcctgcaggtggcgatcaatctaagggcaaaaaccttcatctagatcagcctgttgcagaagaagaaactagcttgctcactggtgaatcacaaccttacaagaggctgcttatggagaagatagaaggatctgaagcggacgtccgcaacccagaaaaagtacggacgtccctccttcagcctctatcgagcagtgacggtggcgtttctgttgccggacagaaccaggccatgatgtggagcagttcaaaagcggctggactcatcggcgaagggtttgaagtcgagctcgatggacttccatggctttcggcgagatcgcaacacatcactgagtttccgatcaccttcgccttcgccacttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

196

Amino Acids

21.15

Weight (kDa)

4.5

Isoelectric Point (pI)

53.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 221
AatII GACGTC 2 cut(s) 369, 395
Acc36I ACCTGC 1 cut(s) 221
AccB7I CCANNNNNTGG 1 cut(s) 462
AccII CGCG 2 cut(s) 171, 196
AciI CCGC 3 cut(s) 362, 370, 479
AclWI GGATC 1 cut(s) 361
AcoI YGGCCR 1 cut(s) 135
AcsI RAATTY 1 cut(s) 130
AcuI CTGAAG 3 cut(s) 122, 378, 386
AcyI GRCGYC 2 cut(s) 366, 392
AfaI GTAC 1 cut(s) 387
AfiI CCNNNNNNNGG 2 cut(s) 233, 462
AgsI TTSAA 3 cut(s) 221, 474, 506
AjnI CCWGG 1 cut(s) 450
AluBI AGCT 3 cut(s) 91, 297, 514
AluI AGCT 3 cut(s) 91, 297, 514
Alw21I GWGCWC 1 cut(s) 516
AlwI GGATC 1 cut(s) 361
AlwNI CAGNNNCTG 1 cut(s) 191
AoxI GGCC 2 cut(s) 135, 453
ApeKI GCWGC 1 cut(s) 331
ApoI RAATTY 1 cut(s) 130
AspLEI GCGC 1 cut(s) 196
AspS9I GGNCC 1 cut(s) 178
AsuHPI GGTGA 2 cut(s) 320, 562
AvaII GGWCC 1 cut(s) 178
BalI TGGCCA 1 cut(s) 137
BanII GRGCYC 1 cut(s) 516
Bbv12I GWGCWC 1 cut(s) 516
BbvI GCAGC 1 cut(s) 318
BccI CCATC 4 cut(s) 68, 189, 197, 512
BciT130I CCWGG 1 cut(s) 452
BfaI CTAG 3 cut(s) 21, 266, 294
BfmI CTRYAG 1 cut(s) 228
BfuAI ACCTGC 1 cut(s) 221
BisI GCNGC 2 cut(s) 332, 480
BlsI GCNGC 2 cut(s) 333, 481
Bme1390I CCNGG 1 cut(s) 452
Bme18I GGWCC 1 cut(s) 178
BmgT120I GGNCC 1 cut(s) 178
BmrFI CCNGG 1 cut(s) 452
BmrI ACTGGG 1 cut(s) 59
BmuI ACTGGG 1 cut(s) 59
BsaHI GRCGYC 2 cut(s) 366, 392
BsaJI CCNNGG 1 cut(s) 527
Bsc4I CCNNNNNNNGG 2 cut(s) 233, 462
Bse1I ACTGG 2 cut(s) 65, 310
Bse3DI GCAATG 1 cut(s) 13
BseBI CCWGG 1 cut(s) 452
BseDI CCNNGG 1 cut(s) 527
BseGI GGATG 2 cut(s) 60, 181
BseLI CCNNNNNNNGG 2 cut(s) 233, 462
BseMI GCAATG 1 cut(s) 13
BseMII CTCAG 1 cut(s) 549
BseNI ACTGG 2 cut(s) 65, 310
BseXI GCAGC 1 cut(s) 318
Bsh1236I CGCG 2 cut(s) 171, 196
Bsh1285I CGRYCG 1 cut(s) 147
BshFI GGCC 2 cut(s) 137, 455
BsiEI CGRYCG 1 cut(s) 147
BsiHKAI GWGCWC 1 cut(s) 516
BsiSI CCGG 1 cut(s) 441
BslFI GGGAC 1 cut(s) 379
BslI CCNNNNNNNGG 2 cut(s) 233, 462
BsmFI GGGAC 1 cut(s) 379
BsnI GGCC 2 cut(s) 137, 455
Bsp1286I GDGCHC 1 cut(s) 516
Bsp143I GATC 8 cut(s) 105, 144, 172, 238, 268, 353, 542, 567
Bsp19I CCATGG 1 cut(s) 527
Bsp68I TCGCGA 1 cut(s) 171
BspACI CCGC 3 cut(s) 362, 370, 479
BspANI GGCC 2 cut(s) 137, 455
BspCNI CTCAG 1 cut(s) 550
BspFNI CGCG 2 cut(s) 171, 196
BspMAI CTGCAG 1 cut(s) 232
BspMI ACCTGC 1 cut(s) 221
BspPI GGATC 1 cut(s) 361
BsrDI GCAATG 1 cut(s) 13
BsrI ACTGG 2 cut(s) 65, 310
BssECI CCNNGG 1 cut(s) 527
BssMI GATC 8 cut(s) 105, 144, 172, 238, 268, 353, 542, 567
BssNI GRCGYC 2 cut(s) 366, 392
BssT1I CCWWGG 1 cut(s) 527
Bst2UI CCWGG 1 cut(s) 452
Bst4CI ACNGT 2 cut(s) 153, 425
BstACI GRCGYC 2 cut(s) 366, 392
BstC8I GCNNGC 4 cut(s) 76, 93, 228, 299
BstDEI CTNAG 2 cut(s) 246, 558
BstDSI CCRYGG 1 cut(s) 527
BstF5I GGATG 2 cut(s) 60, 181
BstFNI CGCG 2 cut(s) 171, 196
BstHHI GCGC 1 cut(s) 196
BstKTI GATC 8 cut(s) 108, 147, 175, 241, 271, 356, 545, 570
BstMBI GATC 8 cut(s) 105, 144, 172, 238, 268, 353, 542, 567
BstMCI CGRYCG 1 cut(s) 147
BstNI CCWGG 1 cut(s) 452
BstSCI CCNGG 1 cut(s) 450
BstSFI CTRYAG 1 cut(s) 228
BstUI CGCG 2 cut(s) 171, 196
BstV1I GCAGC 1 cut(s) 318
BstX2I RGATCY 1 cut(s) 353
BstXI CCANNNNNNTGG 1 cut(s) 67
BstYI RGATCY 1 cut(s) 353
BsuRI GGCC 2 cut(s) 137, 455
BtgI CCRYGG 1 cut(s) 527
BtsCI GGATG 2 cut(s) 60, 181
BtsI GCAGTG 1 cut(s) 424
BtsIMutI CAGTG 4 cut(s) 72, 303, 424, 555
BtuMI TCGCGA 1 cut(s) 171
BveI ACCTGC 1 cut(s) 221
Cac8I GCNNGC 4 cut(s) 76, 93, 228, 299
CaiI CAGNNNCTG 1 cut(s) 191
CfoI GCGC 1 cut(s) 196
Cfr13I GGNCC 1 cut(s) 178
Csp6I GTAC 1 cut(s) 386
CviAII CATG 2 cut(s) 457, 528
CviQI GTAC 1 cut(s) 386
DdeI CTNAG 2 cut(s) 246, 558
DpnI GATC 8 cut(s) 107, 146, 174, 240, 270, 355, 544, 569
DpnII GATC 8 cut(s) 105, 144, 172, 238, 268, 353, 542, 567
EaeI YGGCCR 1 cut(s) 135
Ecl136II GAGCTC 1 cut(s) 514
Eco130I CCWWGG 1 cut(s) 527
Eco24I GRGCYC 1 cut(s) 516
Eco47I GGWCC 1 cut(s) 178
Eco53kI GAGCTC 1 cut(s) 514
Eco57I CTGAAG 3 cut(s) 122, 378, 386
EcoICRI GAGCTC 1 cut(s) 514
EcoRII CCWGG 1 cut(s) 450
EcoT14I CCWWGG 1 cut(s) 527
EcoT38I GRGCYC 1 cut(s) 516
ErhI CCWWGG 1 cut(s) 527
FaeI CATG 2 cut(s) 460, 531
FaiI YATR 3 cut(s) 338, 458, 529
FaqI GGGAC 1 cut(s) 379
FatI CATG 2 cut(s) 456, 527
Fnu4HI GCNGC 2 cut(s) 332, 480
FokI GGATG 2 cut(s) 47, 168
FriOI GRGCYC 1 cut(s) 516
Fsp4HI GCNGC 2 cut(s) 332, 480
FspBI CTAG 3 cut(s) 21, 266, 294
GlaI GCGC 1 cut(s) 195
GluI GCNGC 2 cut(s) 332, 480
HaeIII GGCC 2 cut(s) 137, 455
HapII CCGG 1 cut(s) 441
HhaI GCGC 1 cut(s) 196
Hin1I GRCGYC 2 cut(s) 366, 392
Hin1II CATG 2 cut(s) 460, 531
Hin6I GCGC 1 cut(s) 194
HinP1I GCGC 1 cut(s) 194
HinfI GANTC 2 cut(s) 311, 486
HpaII CCGG 1 cut(s) 441
HphI GGTGA 2 cut(s) 320, 562
Hpy188I TCNGA 3 cut(s) 157, 358, 567
Hpy188III TCNNGA 5 cut(s) 100, 170, 176, 185, 266
HpyAV CCTTC 6 cut(s) 269, 344, 410, 491, 583, 589
HpyCH4III ACNGT 2 cut(s) 153, 425
HpyCH4IV ACGT 2 cut(s) 366, 392
HpyCH4V TGCA 2 cut(s) 230, 281
HpyF3I CTNAG 2 cut(s) 246, 558
HpySE526I ACGT 2 cut(s) 366, 392
Hsp92I GRCGYC 2 cut(s) 366, 392
Hsp92II CATG 2 cut(s) 460, 531
HspAI GCGC 1 cut(s) 194
Kzo9I GATC 8 cut(s) 105, 144, 172, 238, 268, 353, 542, 567
LmnI GCTCC 1 cut(s) 465
Lsp1109I GCAGC 1 cut(s) 318
MaeI CTAG 3 cut(s) 21, 266, 294
MaeII ACGT 2 cut(s) 366, 392
MaeIII GTNAC 2 cut(s) 16, 419
MalI GATC 8 cut(s) 107, 146, 174, 240, 270, 355, 544, 569
MboI GATC 8 cut(s) 105, 144, 172, 238, 268, 353, 542, 567
MboII GAAGA 6 cut(s) 115, 158, 212, 296, 299, 355
MflI RGATCY 1 cut(s) 353
MhlI GDGCHC 1 cut(s) 516
MlsI TGGCCA 1 cut(s) 137
MluCI AATT 2 cut(s) 130, 221
MluNI TGGCCA 1 cut(s) 137
MlyI GAGTC 1 cut(s) 480
MnlI CCTC 6 cut(s) 63, 76, 118, 321, 407, 417
Mox20I TGGCCA 1 cut(s) 137
MscI TGGCCA 1 cut(s) 137
MslI CAYNNNNRTG 1 cut(s) 65
Msp20I TGGCCA 1 cut(s) 137
MspI CCGG 1 cut(s) 441
MspR9I CCNGG 1 cut(s) 452
MvaI CCWGG 1 cut(s) 452
MvnI CGCG 2 cut(s) 171, 196
NcoI CCATGG 1 cut(s) 527
NdeII GATC 8 cut(s) 105, 144, 172, 238, 268, 353, 542, 567
NlaIII CATG 2 cut(s) 460, 531
NmuCI GTSAC 2 cut(s) 16, 419
NruI TCGCGA 1 cut(s) 171
PaqCI CACCTGC 1 cut(s) 221
PfeI GAWTC 1 cut(s) 311
PflMI CCANNNNNTGG 1 cut(s) 462
PkrI GCNGC 2 cut(s) 333, 481
Ple19I CGATCG 1 cut(s) 147
PleI GAGTC 1 cut(s) 480
PpsI GAGTC 1 cut(s) 480
Psp124BI GAGCTC 1 cut(s) 516
Psp6I CCWGG 1 cut(s) 450
PspGI CCWGG 1 cut(s) 450
PspPI GGNCC 1 cut(s) 178
PstI CTGCAG 1 cut(s) 232
PstNI CAGNNNCTG 1 cut(s) 191
PsuI RGATCY 1 cut(s) 353
PvuI CGATCG 1 cut(s) 147
RruI TCGCGA 1 cut(s) 171
RsaI GTAC 1 cut(s) 387
RsaNI GTAC 1 cut(s) 386
RseI CAYNNNNRTG 1 cut(s) 65
SacI GAGCTC 1 cut(s) 516
SatI GCNGC 2 cut(s) 332, 480
Sau3AI GATC 8 cut(s) 105, 144, 172, 238, 268, 353, 542, 567
Sau96I GGNCC 1 cut(s) 178
SbfI CCTGCAGG 1 cut(s) 232
SchI GAGTC 1 cut(s) 480
ScrFI CCNGG 1 cut(s) 452
SdaI CCTGCAGG 1 cut(s) 232
SduI GDGCHC 1 cut(s) 516
SetI ASST 9 cut(s) 93, 235, 261, 299, 322, 369, 395, 516, 575
SfcI CTRYAG 1 cut(s) 228
SinI GGWCC 1 cut(s) 178
SmiMI CAYNNNNRTG 1 cut(s) 65
Sse8387I CCTGCAGG 1 cut(s) 232
Sse9I AATT 2 cut(s) 130, 221
SsiI CCGC 3 cut(s) 362, 370, 479
SspMI CTAG 3 cut(s) 21, 266, 294
SstI GAGCTC 1 cut(s) 516
StyD4I CCNGG 1 cut(s) 450
StyI CCWWGG 1 cut(s) 527
TaaI ACNGT 2 cut(s) 153, 425
TaiI ACGT 2 cut(s) 369, 395
TaqI TCGA 4 cut(s) 143, 413, 510, 516
TasI AATT 2 cut(s) 130, 221
TauI GCSGC 1 cut(s) 482
TfiI GAWTC 1 cut(s) 311
TscAI CASTG 4 cut(s) 72, 310, 424, 562
TseFI GTSAC 2 cut(s) 16, 419
TseI GCWGC 1 cut(s) 331
Tsp45I GTSAC 2 cut(s) 16, 419
TspDTI ATGAA 1 cut(s) 251
TspGWI ACGGA 2 cut(s) 4, 403
TspRI CASTG 4 cut(s) 72, 310, 424, 562
Van91I CCANNNNNTGG 1 cut(s) 462
VpaK11BI GGWCC 1 cut(s) 178
XapI RAATTY 1 cut(s) 130
XbaI TCTAGA 1 cut(s) 265
XspI CTAG 3 cut(s) 21, 266, 294
ZraI GACGTC 2 cut(s) 367, 393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.