Rroxscaffold_176G00430960

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000176
OG0004849
Physical Location & Seq
Forward (+)
43258 .. 48176
4919 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_176G00430960.1

Sequence Viewer

Length: 498 bp
ATGACCCTCCTCTCCTTCACCATCTTCGTCGGCATCAACCTCTTCCACCGGTTCTTGCGGCGCTCCAAATCCCATGAAATTGATGGTGAGATTCAAATAGATTGGGATAGAGAAATGAGAGGGGAATTAAGGAAAACAAGATCGGAGGAAATTGAAAGGAGTGATGGATTTGGAAAGGAAAAAAAATTGAAACTAAAACGTTGGGTCTCGTTAGGGGTGTGCAAAACATGTCCTGTTGCATTAATAGCAGGGATATCGCCTGTAGTAGAGGAAGCAGTAGATTCATACGCAGTTCGAGGTAAAGCACCACCTGAGATTGTACGTGTTAAAACGAGAAACTTCGCAGCCACCCGGCCAGATGCAAAAATGCGAATGGGGCTTAGAACGGCTCAGGGCGGTGTAGATGGCACCGCTAGTCTTGCGGATGCCGAACCAGGCCTCCATCTTGAGCTTCTTCTTGCTAGTGGACTCGTCAGCGATGAGTTCGAAGTCGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

165

Amino Acids

18.35

Weight (kDa)

7.79

Isoelectric Point (pI)

46.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 407
AciI CCGC 4 cut(s) 58, 396, 411, 422
AclI AACGTT 1 cut(s) 199
AcoI YGGCCR 1 cut(s) 353
AfaI GTAC 1 cut(s) 321
AflIII ACRYGT 2 cut(s) 227, 322
AgeI ACCGGT 1 cut(s) 48
AgsI TTSAA 3 cut(s) 95, 155, 190
AjnI CCWGG 1 cut(s) 433
AloI GAACNNNNNNTCC 2 cut(s) 423, 455
AluBI AGCT 1 cut(s) 451
AluI AGCT 1 cut(s) 451
Alw26I GTCTC 1 cut(s) 211
AoxI GGCC 2 cut(s) 353, 436
ApeKI GCWGC 1 cut(s) 344
AseI ATTAAT 1 cut(s) 242
AsiGI ACCGGT 1 cut(s) 48
AspLEI GCGC 1 cut(s) 63
AsuC2I CCSGG 1 cut(s) 352
AsuHPI GGTGA 2 cut(s) 10, 98
AsuII TTCGAA 1 cut(s) 486
BanI GGYRCC 1 cut(s) 407
BbvI GCAGC 1 cut(s) 356
BccI CCATC 5 cut(s) 29, 77, 158, 398, 450
BceAI ACGGC 1 cut(s) 402
BcgI CGANNNNNNTGC 2 cut(s) 237, 271
BciT130I CCWGG 1 cut(s) 435
BcnI CCSGG 1 cut(s) 352
BcoDI GTCTC 1 cut(s) 211
BfaI CTAG 2 cut(s) 414, 462
BfmI CTRYAG 1 cut(s) 261
BfoI RGCGCY 1 cut(s) 64
BisI GCNGC 2 cut(s) 59, 345
BlsI GCNGC 2 cut(s) 60, 346
Bme1390I CCNGG 2 cut(s) 352, 435
BmiI GGNNCC 1 cut(s) 409
BmrFI CCNGG 2 cut(s) 352, 435
BmsI GCATC 3 cut(s) 42, 349, 415
Bpu10I CCTNAGC 1 cut(s) 390
Bpu14I TTCGAA 1 cut(s) 486
BpuEI CTTGAG 1 cut(s) 467
BpuMI CCSGG 1 cut(s) 352
BsaAI YACGTR 1 cut(s) 323
BsaI GGTCTC 1 cut(s) 211
BsaWI WCCGGW 1 cut(s) 48
BsaXI ACNNNNNCTCC 2 cut(s) 423, 453
Bse118I RCCGGY 1 cut(s) 48
BseBI CCWGG 1 cut(s) 435
BseGI GGATG 1 cut(s) 430
BseMII CTCAG 2 cut(s) 303, 404
BseXI GCAGC 1 cut(s) 356
BshFI GGCC 2 cut(s) 355, 438
BshNI GGYRCC 1 cut(s) 407
BshTI ACCGGT 1 cut(s) 48
BsiSI CCGG 2 cut(s) 49, 352
BsmAI GTCTC 1 cut(s) 211
BsnI GGCC 2 cut(s) 355, 438
Bso31I GGTCTC 1 cut(s) 211
Bsp119I TTCGAA 1 cut(s) 486
Bsp143I GATC 1 cut(s) 140
BspACI CCGC 4 cut(s) 58, 396, 411, 422
BspANI GGCC 2 cut(s) 355, 438
BspCNI CTCAG 2 cut(s) 304, 403
BspLI GGNNCC 1 cut(s) 409
BspT104I TTCGAA 1 cut(s) 486
BspT107I GGYRCC 1 cut(s) 407
BspTNI GGTCTC 1 cut(s) 211
BsrFI RCCGGY 1 cut(s) 48
BssAI RCCGGY 1 cut(s) 48
BssMI GATC 1 cut(s) 140
Bst2UI CCWGG 1 cut(s) 435
Bst6I CTCTTC 1 cut(s) 47
BstBAI YACGTR 1 cut(s) 323
BstBI TTCGAA 1 cut(s) 486
BstDEI CTNAG 3 cut(s) 312, 380, 390
BstF5I GGATG 1 cut(s) 430
BstH2I RGCGCY 1 cut(s) 64
BstHHI GCGC 1 cut(s) 63
BstKTI GATC 1 cut(s) 143
BstMAI GTCTC 1 cut(s) 211
BstMBI GATC 1 cut(s) 140
BstMWI GCNNNNNNNGC 3 cut(s) 245, 376, 419
BstNI CCWGG 1 cut(s) 435
BstNSI RCATGY 1 cut(s) 231
BstSCI CCNGG 2 cut(s) 350, 433
BstSFI CTRYAG 1 cut(s) 261
BstV1I GCAGC 1 cut(s) 356
BsuRI GGCC 2 cut(s) 355, 438
BtgZI GCGATG 1 cut(s) 492
BtsCI GGATG 1 cut(s) 430
CfoI GCGC 1 cut(s) 63
Cfr10I RCCGGY 1 cut(s) 48
Csp6I GTAC 1 cut(s) 320
CspAI ACCGGT 1 cut(s) 48
CviAII CATG 2 cut(s) 74, 228
CviJI RGCY 6 cut(s) 347, 355, 379, 389, 438, 451
CviKI_1 RGCY 6 cut(s) 347, 355, 379, 389, 438, 451
CviQI GTAC 1 cut(s) 320
DdeI CTNAG 3 cut(s) 312, 380, 390
DpnI GATC 1 cut(s) 142
DpnII GATC 1 cut(s) 140
EaeI YGGCCR 1 cut(s) 353
Eam1104I CTCTTC 1 cut(s) 47
EarI CTCTTC 1 cut(s) 47
Eco147I AGGCCT 1 cut(s) 438
Eco31I GGTCTC 1 cut(s) 211
Eco32I GATATC 1 cut(s) 255
EcoRII CCWGG 1 cut(s) 433
EcoRV GATATC 1 cut(s) 255
FaeI CATG 2 cut(s) 77, 231
FaiI YATR 3 cut(s) 75, 229, 286
FatI CATG 2 cut(s) 73, 227
Fnu4HI GCNGC 2 cut(s) 59, 345
FokI GGATG 1 cut(s) 437
Fsp4HI GCNGC 2 cut(s) 59, 345
FspBI CTAG 2 cut(s) 414, 462
GlaI GCGC 1 cut(s) 62
GluI GCNGC 2 cut(s) 59, 345
HaeII RGCGCY 1 cut(s) 64
HaeIII GGCC 2 cut(s) 355, 438
HapII CCGG 2 cut(s) 49, 352
HhaI GCGC 1 cut(s) 63
Hin1II CATG 2 cut(s) 77, 231
Hin6I GCGC 1 cut(s) 61
HinP1I GCGC 1 cut(s) 61
HinfI GANTC 3 cut(s) 91, 281, 468
HpaII CCGG 2 cut(s) 49, 352
HphI GGTGA 2 cut(s) 10, 98
Hpy166II GTNNAC 1 cut(s) 467
Hpy188I TCNGA 1 cut(s) 145
Hpy188III TCNNGA 1 cut(s) 446
Hpy8I GTNNAC 1 cut(s) 467
Hpy99I CGWCG 1 cut(s) 32
HpyAV CCTTC 1 cut(s) 25
HpyCH4IV ACGT 2 cut(s) 199, 322
HpyCH4V TGCA 3 cut(s) 222, 239, 362
HpyF10VI GCNNNNNNNGC 3 cut(s) 245, 376, 419
HpyF3I CTNAG 3 cut(s) 312, 380, 390
HpySE526I ACGT 2 cut(s) 199, 322
Hsp92II CATG 2 cut(s) 77, 231
HspAI GCGC 1 cut(s) 61
Kzo9I GATC 1 cut(s) 140
LmnI GCTCC 1 cut(s) 68
Lsp1109I GCAGC 1 cut(s) 356
LweI GCATC 3 cut(s) 42, 349, 415
MaeI CTAG 2 cut(s) 414, 462
MaeII ACGT 2 cut(s) 199, 322
MalI GATC 1 cut(s) 142
MboI GATC 1 cut(s) 140
MboII GAAGA 3 cut(s) 16, 34, 446
MluCI AATT 4 cut(s) 78, 125, 150, 185
MlyI GAGTC 1 cut(s) 462
MnlI CCTC 8 cut(s) 17, 20, 50, 113, 139, 262, 290, 449
MseI TTAA 3 cut(s) 128, 242, 327
MspI CCGG 2 cut(s) 49, 352
MspR9I CCNGG 2 cut(s) 352, 435
MvaI CCWGG 1 cut(s) 435
MwoI GCNNNNNNNGC 3 cut(s) 245, 376, 419
NciI CCSGG 1 cut(s) 352
NdeII GATC 1 cut(s) 140
NlaIII CATG 2 cut(s) 77, 231
NlaIV GGNNCC 1 cut(s) 409
NspI RCATGY 1 cut(s) 231
NspV TTCGAA 1 cut(s) 486
PceI AGGCCT 1 cut(s) 438
PciI ACATGT 1 cut(s) 227
PfeI GAWTC 2 cut(s) 91, 281
PinAI ACCGGT 1 cut(s) 48
PkrI GCNGC 2 cut(s) 60, 346
PleI GAGTC 1 cut(s) 462
PpsI GAGTC 1 cut(s) 462
Ppu21I YACGTR 1 cut(s) 323
PscI ACATGT 1 cut(s) 227
PshBI ATTAAT 1 cut(s) 242
Psp1406I AACGTT 1 cut(s) 199
Psp6I CCWGG 1 cut(s) 433
PspGI CCWGG 1 cut(s) 433
PspN4I GGNNCC 1 cut(s) 409
RsaI GTAC 1 cut(s) 321
RsaNI GTAC 1 cut(s) 320
SaqAI TTAA 3 cut(s) 128, 242, 327
SatI GCNGC 2 cut(s) 59, 345
Sau3AI GATC 1 cut(s) 140
SchI GAGTC 1 cut(s) 462
ScrFI CCNGG 2 cut(s) 352, 435
SetI ASST 6 cut(s) 42, 202, 301, 313, 325, 453
SfaNI GCATC 3 cut(s) 42, 349, 415
SfcI CTRYAG 1 cut(s) 261
SfuI TTCGAA 1 cut(s) 486
SmlI CTYRAG 1 cut(s) 446
SmoI CTYRAG 1 cut(s) 446
Sse9I AATT 4 cut(s) 78, 125, 150, 185
SseBI AGGCCT 1 cut(s) 438
SsiI CCGC 4 cut(s) 58, 396, 411, 422
SspMI CTAG 2 cut(s) 414, 462
StuI AGGCCT 1 cut(s) 438
StyD4I CCNGG 2 cut(s) 350, 433
TaiI ACGT 2 cut(s) 202, 325
TaqI TCGA 3 cut(s) 295, 486, 492
TasI AATT 4 cut(s) 78, 125, 150, 185
TauI GCSGC 1 cut(s) 61
TfiI GAWTC 2 cut(s) 91, 281
Tru1I TTAA 3 cut(s) 128, 242, 327
Tru9I TTAA 3 cut(s) 128, 242, 327
TseI GCWGC 1 cut(s) 344
TspDTI ATGAA 2 cut(s) 90, 273
VspI ATTAAT 1 cut(s) 242
XceI RCATGY 1 cut(s) 231
XcmI CCANNNNNNNNNTGG 1 cut(s) 80
XspI CTAG 2 cut(s) 414, 462
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.