Rh1DG437400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0004849
Physical Location & Seq
Forward (+)
64776681 .. 64777614
934 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG437400.1

Sequence Viewer

Length: 387 bp
ATGGAGGAGTCCACCGGGAAGAAGAATCTCAAGATGGAGGCCTGGTTTGGCACGAGCAAAGATCAGCTCCACCATCCGAGCAGCCTCGAGCCGTTCTTAGCAGCGTCCGCACTTTTACATCTTTGCGGACGTCCGCCTCTGATCCGACCTGATATACGGAGCCGTTCAAAAGAGGACGTTAGCACAGATGCCAAATTGCGGATGTCCTTCCTCAAGCCTCGATCAAGTAGCGATGGCGGCTCTGAGGTTGCTAGTGCCAAACCAGACCTCAATGTCGAGTCTTTGCTTGCCGCTGGAGTCGTCTATGAAGAGTTCGAAGTCGACCTTGTTGGGCTTCCATGGTTTCGGGCGAGCTCACCGCGCACATCTTCGATTCCGATTAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

128

Amino Acids

14.0

Weight (kDa)

6.29

Isoelectric Point (pI)

51.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 272
AatII GACGTC 1 cut(s) 133
AccI GTMKAC 1 cut(s) 321
AccII CGCG 1 cut(s) 361
AciI CCGC 7 cut(s) 108, 126, 134, 199, 237, 291, 359
AclWI GGATC 1 cut(s) 136
AcyI GRCGYC 1 cut(s) 130
AfiI CCNNNNNNNGG 1 cut(s) 198
AgsI TTSAA 1 cut(s) 168
AjnI CCWGG 1 cut(s) 41
AluBI AGCT 3 cut(s) 67, 354, 384
AluI AGCT 3 cut(s) 67, 354, 384
Alw21I GWGCWC 1 cut(s) 356
AlwI GGATC 1 cut(s) 136
Ama87I CYCGRG 1 cut(s) 86
AoxI GGCC 1 cut(s) 39
ApeKI GCWGC 2 cut(s) 81, 101
AspLEI GCGC 1 cut(s) 363
AsuC2I CCSGG 1 cut(s) 16
AsuHPI GGTGA 1 cut(s) 348
AsuII TTCGAA 1 cut(s) 315
AvaI CYCGRG 1 cut(s) 86
BanII GRGCYC 1 cut(s) 356
BauI CACGAG 1 cut(s) 52
Bbv12I GWGCWC 1 cut(s) 356
BbvI GCAGC 2 cut(s) 93, 113
BccI CCATC 3 cut(s) 28, 81, 227
BceAI ACGGC 2 cut(s) 76, 147
BciT130I CCWGG 1 cut(s) 43
BcnI CCSGG 1 cut(s) 16
BfaI CTAG 1 cut(s) 252
BisI GCNGC 4 cut(s) 82, 102, 238, 291
BlsI GCNGC 4 cut(s) 83, 103, 239, 292
Bme1390I CCNGG 2 cut(s) 16, 43
BmeT110I CYCGRG 1 cut(s) 86
BmiI GGNNCC 1 cut(s) 161
BmrFI CCNGG 2 cut(s) 16, 43
BmsI GCATC 1 cut(s) 178
BpmI CTGGAG 1 cut(s) 315
Bpu14I TTCGAA 1 cut(s) 315
BpuEI CTTGAG 2 cut(s) 14, 197
BpuMI CCSGG 1 cut(s) 16
BsaHI GRCGYC 1 cut(s) 130
BsaJI CCNNGG 1 cut(s) 338
BsaXI ACNNNNNCTCC 2 cut(s) 29, 59
Bsc4I CCNNNNNNNGG 1 cut(s) 198
BseBI CCWGG 1 cut(s) 43
BseDI CCNNGG 1 cut(s) 338
BseGI GGATG 2 cut(s) 73, 207
BseLI CCNNNNNNNGG 1 cut(s) 198
BseMII CTCAG 1 cut(s) 234
BseRI GAGGAG 1 cut(s) 20
BseXI GCAGC 2 cut(s) 93, 113
Bsh1236I CGCG 1 cut(s) 361
BshFI GGCC 1 cut(s) 41
BsiHKAI GWGCWC 1 cut(s) 356
BsiHKCI CYCGRG 1 cut(s) 86
BsiSI CCGG 1 cut(s) 15
BslI CCNNNNNNNGG 1 cut(s) 198
BsnI GGCC 1 cut(s) 41
BsoBI CYCGRG 1 cut(s) 86
Bsp119I TTCGAA 1 cut(s) 315
Bsp1286I GDGCHC 1 cut(s) 356
Bsp143I GATC 3 cut(s) 61, 141, 221
Bsp19I CCATGG 1 cut(s) 338
BspACI CCGC 7 cut(s) 108, 126, 134, 199, 237, 291, 359
BspANI GGCC 1 cut(s) 41
BspCNI CTCAG 1 cut(s) 235
BspFNI CGCG 1 cut(s) 361
BspLI GGNNCC 1 cut(s) 161
BspPI GGATC 1 cut(s) 136
BspT104I TTCGAA 1 cut(s) 315
BssECI CCNNGG 1 cut(s) 338
BssMI GATC 3 cut(s) 61, 141, 221
BssNI GRCGYC 1 cut(s) 130
BssSI CACGAG 1 cut(s) 52
BssT1I CCWWGG 1 cut(s) 338
Bst2BI CACGAG 1 cut(s) 52
Bst2UI CCWGG 1 cut(s) 43
Bst6I CTCTTC 1 cut(s) 303
BstACI GRCGYC 1 cut(s) 130
BstBI TTCGAA 1 cut(s) 315
BstC8I GCNNGC 2 cut(s) 288, 352
BstDEI CTNAG 2 cut(s) 97, 243
BstDSI CCRYGG 1 cut(s) 338
BstF5I GGATG 2 cut(s) 73, 207
BstFNI CGCG 1 cut(s) 361
BstHHI GCGC 1 cut(s) 363
BstKTI GATC 3 cut(s) 64, 144, 224
BstMBI GATC 3 cut(s) 61, 141, 221
BstMWI GCNNNNNNNGC 3 cut(s) 107, 237, 360
BstNI CCWGG 1 cut(s) 43
BstSCI CCNGG 2 cut(s) 14, 41
BstUI CGCG 1 cut(s) 361
BstV1I GCAGC 2 cut(s) 93, 113
BsuRI GGCC 1 cut(s) 41
BtgI CCRYGG 1 cut(s) 338
BtgZI GCGATG 1 cut(s) 246
BtsCI GGATG 2 cut(s) 73, 207
Cac8I GCNNGC 2 cut(s) 288, 352
CfoI GCGC 1 cut(s) 363
CseI GACGC 1 cut(s) 93
CviAII CATG 1 cut(s) 339
DdeI CTNAG 2 cut(s) 97, 243
DpnI GATC 3 cut(s) 63, 143, 223
DpnII GATC 3 cut(s) 61, 141, 221
DrdI GACNNNNNNGTC 1 cut(s) 272
DseDI GACNNNNNNGTC 1 cut(s) 272
Eam1104I CTCTTC 1 cut(s) 303
EarI CTCTTC 1 cut(s) 303
EciI GGCGGA 1 cut(s) 123
Ecl136II GAGCTC 1 cut(s) 354
Eco130I CCWWGG 1 cut(s) 338
Eco147I AGGCCT 1 cut(s) 41
Eco24I GRGCYC 1 cut(s) 356
Eco53kI GAGCTC 1 cut(s) 354
Eco88I CYCGRG 1 cut(s) 86
EcoICRI GAGCTC 1 cut(s) 354
EcoRII CCWGG 1 cut(s) 41
EcoT14I CCWWGG 1 cut(s) 338
EcoT38I GRGCYC 1 cut(s) 356
ErhI CCWWGG 1 cut(s) 338
FaeI CATG 1 cut(s) 342
FaiI YATR 3 cut(s) 155, 306, 340
FalI AAGNNNNNCTT 2 cut(s) 309, 341
FatI CATG 1 cut(s) 338
FblI GTMKAC 1 cut(s) 321
Fnu4HI GCNGC 4 cut(s) 82, 102, 238, 291
FokI GGATG 2 cut(s) 60, 214
FriOI GRGCYC 1 cut(s) 356
Fsp4HI GCNGC 4 cut(s) 82, 102, 238, 291
FspBI CTAG 1 cut(s) 252
GlaI GCGC 1 cut(s) 362
GluI GCNGC 4 cut(s) 82, 102, 238, 291
GsuI CTGGAG 1 cut(s) 315
HaeIII GGCC 1 cut(s) 41
HapII CCGG 1 cut(s) 15
HgaI GACGC 1 cut(s) 93
HhaI GCGC 1 cut(s) 363
Hin1I GRCGYC 1 cut(s) 130
Hin1II CATG 1 cut(s) 342
Hin6I GCGC 1 cut(s) 361
HinP1I GCGC 1 cut(s) 361
HincII GTYRAC 1 cut(s) 322
HindII GTYRAC 1 cut(s) 322
HinfI GANTC 5 cut(s) 8, 25, 278, 297, 373
HpaII CCGG 1 cut(s) 15
HphI GGTGA 1 cut(s) 348
Hpy166II GTNNAC 2 cut(s) 12, 322
Hpy188I TCNGA 5 cut(s) 78, 141, 146, 244, 378
Hpy188III TCNNGA 1 cut(s) 31
Hpy8I GTNNAC 2 cut(s) 12, 322
HpyAV CCTTC 1 cut(s) 217
HpyCH4IV ACGT 2 cut(s) 130, 177
HpyF10VI GCNNNNNNNGC 3 cut(s) 107, 237, 360
HpyF3I CTNAG 2 cut(s) 97, 243
HpySE526I ACGT 2 cut(s) 130, 177
Hsp92I GRCGYC 1 cut(s) 130
Hsp92II CATG 1 cut(s) 342
HspAI GCGC 1 cut(s) 361
Kzo9I GATC 3 cut(s) 61, 141, 221
LmnI GCTCC 2 cut(s) 72, 159
LpnPI CCDG 6 cut(s) 28, 28, 55, 162, 276, 279
Lsp1109I GCAGC 2 cut(s) 93, 113
LweI GCATC 1 cut(s) 178
MaeI CTAG 1 cut(s) 252
MaeII ACGT 2 cut(s) 130, 177
MalI GATC 3 cut(s) 63, 143, 223
MboI GATC 3 cut(s) 61, 141, 221
MboII GAAGA 4 cut(s) 31, 34, 320, 360
MhlI GDGCHC 1 cut(s) 356
MluCI AATT 1 cut(s) 194
MlyI GAGTC 3 cut(s) 17, 287, 306
MmeI TCCRAC 1 cut(s) 169
MnlI CCTC 8 cut(s) 31, 95, 147, 166, 221, 228, 238, 278
MspA1I CMGCKG 1 cut(s) 293
MspI CCGG 1 cut(s) 15
MspR9I CCNGG 2 cut(s) 16, 43
MvaI CCWGG 1 cut(s) 43
MvnI CGCG 1 cut(s) 361
MwoI GCNNNNNNNGC 3 cut(s) 107, 237, 360
NciI CCSGG 1 cut(s) 16
NcoI CCATGG 1 cut(s) 338
NdeII GATC 3 cut(s) 61, 141, 221
NlaIII CATG 1 cut(s) 342
NlaIV GGNNCC 1 cut(s) 161
NspV TTCGAA 1 cut(s) 315
PaeR7I CTCGAG 1 cut(s) 86
PceI AGGCCT 1 cut(s) 41
PfeI GAWTC 2 cut(s) 25, 373
PkrI GCNGC 4 cut(s) 83, 103, 239, 292
PleI GAGTC 3 cut(s) 16, 286, 305
PpsI GAGTC 3 cut(s) 16, 286, 305
Psp124BI GAGCTC 1 cut(s) 356
Psp6I CCWGG 1 cut(s) 41
PspGI CCWGG 1 cut(s) 41
PspN4I GGNNCC 1 cut(s) 161
PspXI VCTCGAGB 1 cut(s) 86
SacI GAGCTC 1 cut(s) 356
SalI GTCGAC 1 cut(s) 320
SatI GCNGC 4 cut(s) 82, 102, 238, 291
Sau3AI GATC 3 cut(s) 61, 141, 221
SchI GAGTC 3 cut(s) 17, 287, 306
ScrFI CCNGG 2 cut(s) 16, 43
SduI GDGCHC 1 cut(s) 356
SetI ASST 9 cut(s) 69, 133, 151, 180, 249, 270, 327, 356, 386
SfaNI GCATC 1 cut(s) 178
Sfr274I CTCGAG 1 cut(s) 86
SfuI TTCGAA 1 cut(s) 315
SlaI CTCGAG 1 cut(s) 86
SmlI CTYRAG 3 cut(s) 29, 86, 212
SmoI CTYRAG 3 cut(s) 29, 86, 212
Sse9I AATT 1 cut(s) 194
SseBI AGGCCT 1 cut(s) 41
SsiI CCGC 7 cut(s) 108, 126, 134, 199, 237, 291, 359
SspMI CTAG 1 cut(s) 252
SstI GAGCTC 1 cut(s) 356
StuI AGGCCT 1 cut(s) 41
StyD4I CCNGG 2 cut(s) 14, 41
StyI CCWWGG 1 cut(s) 338
TaiI ACGT 2 cut(s) 133, 180
TaqI TCGA 6 cut(s) 87, 220, 276, 315, 321, 371
TasI AATT 1 cut(s) 194
TauI GCSGC 2 cut(s) 240, 293
TfiI GAWTC 2 cut(s) 25, 373
TseI GCWGC 2 cut(s) 81, 101
TspDTI ATGAA 1 cut(s) 321
TspGWI ACGGA 1 cut(s) 172
XhoI CTCGAG 1 cut(s) 86
XmiI GTMKAC 1 cut(s) 321
XspI CTAG 1 cut(s) 252
ZraI GACGTC 1 cut(s) 131
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.