Rmu_sc0006282.1_g000034

LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006282.1
OG0002877
Physical Location & Seq
Reverse (-)
135950 .. 137982
2033 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1602 bp
atggagcccaagtgtgagttgggagcaatgacaagttctcatgttctaggaacttctggtgagagtatcatcatcgacagatttagtgatctcccagatgaaattgctcatcacattctttccttccactgtggcatcttaatgaacctcatccgagtgggcgcagtgtccaagagatgcagacaactttatctatcacttccgtctttgattattgtttcaggtcatgtttcaaactgcaccatgtcgagagtaaggttgatgaattattgggataggtacttgtttcatcgtggtgataataagatagagtgctttggcatttgctggtctttcgatgccgcagtcaatgcagagataagtgatgagttttttcgaatcaccacgtggatccaaaatgcagtaaggtgtaatgttaaagtgcttcaccttgaattcactcaaaacaacttgaatatagttgtgttaccatcttgcatctttcattgtcattctttgacggaactctatgtgaacttgagtgggggtgatattcaatcaccctccttgtctttttcaagtaatctccagcacttggagttgaaggatgctatggttgaagatgaccggtttttcgaatggatatcatcttcctgcaaatgcatcaaggaattacgtcttgaaaatgttagtggaataaaagatatcacgattgagagctcgtctttggaaatatttatttatctgtttccttttaacaattatgagctctgggatcttaacatttcaggggagaaacttgaagaaataataatactttggcattttgattcgaatgatgattcaagacgtggtcttatacctcgcatcaggtcgctaaatattctcgccccagagcttaaaaagatcacgtggggtgggaactttctggatcatcagcatcttgggaacttgatgtgcttagaagaagctaagatttttctgttccccgacttagatgacttcaataacccatttgaggttctctgcagtattcgcgtggttaaagctcttgtcctaaacgaagagactacaaaggcttttttcatggaaagttcgatgccattacgattagataatattcatgacttgactttacatgttagaagcgtgaatgatgacttcgtcccagcaatggtctctcttttcagaggaatcactactttgaatactctatgcataacgtctgtcacatacaagcaagcaattgaggaagcgtataatgaaactgaggaagaggataataattatgaggaagaggacaacggaactgagcagaagaatagtgagactacagaagaggatggtgcaactgaggaaggggataatgctcccaagtttgatgtggaatattggaaatcccaaaaccttaattttacttgtgagctaaaggaggtgaaactggagattgtggagggtaataataatgaattggatttagcaaggaacattctggagcatgctcagaaattgaagaaaatggtgattcttcattcaccgcagcatccggatattttggaaatggtaagaaaaagcaagatgatatccactgcaacagtggttttgcaagaagtaatattgtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

533

Amino Acids

61.1

Weight (kDa)

4.87

Isoelectric Point (pI)

52.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At5g56370 PF24758 128 - 302 1.2e-09 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 574
AccII CGCG 1 cut(s) 1017
AccIII TCCGGA 1 cut(s) 1525
AciI CCGC 2 cut(s) 342, 1517
AclWI GGATC 4 cut(s) 385, 398, 762, 918
AcsI RAATTY 1 cut(s) 434
AcvI CACGTG 2 cut(s) 387, 891
AdeI CACNNNGTG 1 cut(s) 387
AfaI GTAC 1 cut(s) 281
AfiI CCNNNNNNNGG 3 cut(s) 574, 997, 1153
AflIII ACRYGT 1 cut(s) 1117
AgeI ACCGGT 1 cut(s) 606
AjiI CACGTC 1 cut(s) 830
AluBI AGCT 6 cut(s) 699, 748, 877, 950, 1028, 1405
AluI AGCT 6 cut(s) 699, 748, 877, 950, 1028, 1405
Alw21I GWGCWC 2 cut(s) 701, 750
Alw26I GTCTC 3 cut(s) 1040, 1162, 1301
AlwI GGATC 4 cut(s) 385, 398, 762, 918
Aor13HI TCCGGA 1 cut(s) 1525
ApeKI GCWGC 1 cut(s) 1519
ApoI RAATTY 1 cut(s) 434
ArsI GACNNNNNNTTYG 2 cut(s) 596, 628
AsiGI ACCGGT 1 cut(s) 606
AspLEI GCGC 1 cut(s) 164
AsuHPI GGTGA 9 cut(s) 71, 308, 373, 419, 531, 539, 1426, 1506, 1513
AsuII TTCGAA 3 cut(s) 376, 615, 812
BamHI GGATCC 1 cut(s) 390
BanII GRGCYC 3 cut(s) 9, 701, 750
BbrPI CACGTG 2 cut(s) 387, 891
Bbv12I GWGCWC 2 cut(s) 701, 750
BbvI GCAGC 1 cut(s) 1531
BccI CCATC 2 cut(s) 478, 1316
BcoDI GTCTC 3 cut(s) 1040, 1162, 1301
BfaI CTAG 1 cut(s) 47
BfmI CTRYAG 2 cut(s) 1006, 1311
BisI GCNGC 2 cut(s) 342, 1520
BlsI GCNGC 2 cut(s) 343, 1521
BmgBI CACGTC 1 cut(s) 830
BmiI GGNNCC 2 cut(s) 6, 392
BpmI CTGGAG 3 cut(s) 551, 1442, 1493
Bpu14I TTCGAA 3 cut(s) 376, 615, 812
BpuEI CTTGAG 1 cut(s) 538
BsaAI YACGTR 2 cut(s) 387, 891
BsaI GGTCTC 1 cut(s) 1162
BsaWI WCCGGW 2 cut(s) 606, 1525
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 3 cut(s) 574, 997, 1153
Bse118I RCCGGY 1 cut(s) 606
Bse1I ACTGG 1 cut(s) 1425
Bse3DI GCAATG 2 cut(s) 33, 1158
BseAI TCCGGA 1 cut(s) 1525
BseGI GGATG 4 cut(s) 150, 592, 1327, 1522
BseLI CCNNNNNNNGG 3 cut(s) 574, 997, 1153
BseMI GCAATG 2 cut(s) 33, 1158
BseMII CTCAG 4 cut(s) 1239, 1281, 1323, 1496
BseNI ACTGG 1 cut(s) 1425
BseXI GCAGC 1 cut(s) 1531
BseYI CCCAGC 1 cut(s) 1147
BsgI GTGCAG 1 cut(s) 223
Bsh1236I CGCG 1 cut(s) 1017
BshTI ACCGGT 1 cut(s) 606
BsiHKAI GWGCWC 2 cut(s) 701, 750
BsiSI CCGG 2 cut(s) 607, 1526
BslFI GGGAC 1 cut(s) 1130
BslI CCNNNNNNNGG 3 cut(s) 574, 997, 1153
BsmAI GTCTC 3 cut(s) 1040, 1162, 1301
BsmFI GGGAC 1 cut(s) 1130
Bso31I GGTCTC 1 cut(s) 1162
Bsp119I TTCGAA 3 cut(s) 376, 615, 812
Bsp1286I GDGCHC 3 cut(s) 9, 701, 750
Bsp13I TCCGGA 1 cut(s) 1525
Bsp143I GATC 5 cut(s) 88, 390, 754, 885, 910
BspACI CCGC 2 cut(s) 342, 1517
BspCNI CTCAG 4 cut(s) 1240, 1282, 1324, 1495
BspEI TCCGGA 1 cut(s) 1525
BspFNI CGCG 1 cut(s) 1017
BspHI TCATGA 1 cut(s) 1102
BspLI GGNNCC 2 cut(s) 6, 392
BspMAI CTGCAG 1 cut(s) 1010
BspPI GGATC 4 cut(s) 385, 398, 762, 918
BspT104I TTCGAA 3 cut(s) 376, 615, 812
BspTNI GGTCTC 1 cut(s) 1162
BsrDI GCAATG 2 cut(s) 33, 1158
BsrFI RCCGGY 1 cut(s) 606
BsrI ACTGG 1 cut(s) 1425
BssAI RCCGGY 1 cut(s) 606
BssMI GATC 5 cut(s) 88, 390, 754, 885, 910
Bst4CI ACNGT 2 cut(s) 131, 1576
Bst6I CTCTTC 4 cut(s) 1038, 1248, 1269, 1311
BstAPI GCANNNNNTGC 1 cut(s) 350
BstBAI YACGTR 2 cut(s) 387, 891
BstBI TTCGAA 3 cut(s) 376, 615, 812
BstC8I GCNNGC 2 cut(s) 1221, 1479
BstDEI CTNAG 7 cut(s) 940, 951, 973, 1248, 1290, 1332, 1482
BstF5I GGATG 4 cut(s) 150, 592, 1327, 1522
BstFNI CGCG 1 cut(s) 1017
BstHHI GCGC 1 cut(s) 164
BstKTI GATC 5 cut(s) 91, 393, 757, 888, 913
BstMAI GTCTC 3 cut(s) 1040, 1162, 1301
BstMBI GATC 5 cut(s) 88, 390, 754, 885, 910
BstMWI GCNNNNNNNGC 2 cut(s) 350, 1014
BstNSI RCATGY 2 cut(s) 1121, 1481
BstSFI CTRYAG 2 cut(s) 1006, 1311
BstUI CGCG 1 cut(s) 1017
BstV1I GCAGC 1 cut(s) 1531
BstX2I RGATCY 2 cut(s) 390, 754
BstYI RGATCY 2 cut(s) 390, 754
BtrI CACGTC 1 cut(s) 830
BtsCI GGATG 4 cut(s) 150, 592, 1327, 1522
BtsI GCAGTG 2 cut(s) 171, 1566
BtsIMutI CAGTG 4 cut(s) 127, 171, 1566, 1581
Cac8I GCNNGC 2 cut(s) 1221, 1479
CciI TCATGA 1 cut(s) 1102
CfoI GCGC 1 cut(s) 164
Cfr10I RCCGGY 1 cut(s) 606
Csp6I GTAC 1 cut(s) 280
CspAI ACCGGT 1 cut(s) 606
CviAII CATG 7 cut(s) 41, 227, 244, 1066, 1103, 1118, 1478
CviJI RGCY 8 cut(s) 7, 699, 748, 877, 950, 1028, 1058, 1405
CviKI_1 RGCY 8 cut(s) 7, 699, 748, 877, 950, 1028, 1058, 1405
CviQI GTAC 1 cut(s) 280
DdeI CTNAG 7 cut(s) 940, 951, 973, 1248, 1290, 1332, 1482
DpnI GATC 5 cut(s) 90, 392, 756, 887, 912
DpnII GATC 5 cut(s) 88, 390, 754, 885, 910
DraIII CACNNNGTG 1 cut(s) 387
Eam1104I CTCTTC 4 cut(s) 1038, 1248, 1269, 1311
EarI CTCTTC 4 cut(s) 1038, 1248, 1269, 1311
Ecl136II GAGCTC 2 cut(s) 699, 748
Eco24I GRGCYC 3 cut(s) 9, 701, 750
Eco31I GGTCTC 1 cut(s) 1162
Eco32I GATATC 3 cut(s) 624, 685, 1563
Eco53kI GAGCTC 2 cut(s) 699, 748
Eco72I CACGTG 2 cut(s) 387, 891
EcoICRI GAGCTC 2 cut(s) 699, 748
EcoRI GAATTC 1 cut(s) 434
EcoRV GATATC 3 cut(s) 624, 685, 1563
EcoT22I ATGCAT 2 cut(s) 644, 1199
EcoT38I GRGCYC 3 cut(s) 9, 701, 750
FaeI CATG 7 cut(s) 44, 230, 247, 1069, 1106, 1121, 1481
FaqI GGGAC 1 cut(s) 1130
FatI CATG 7 cut(s) 40, 226, 243, 1065, 1102, 1117, 1477
Fnu4HI GCNGC 2 cut(s) 342, 1520
FokI GGATG 4 cut(s) 137, 599, 1334, 1509
FriOI GRGCYC 3 cut(s) 9, 701, 750
Fsp4HI GCNGC 2 cut(s) 342, 1520
FspBI CTAG 1 cut(s) 47
GlaI GCGC 1 cut(s) 163
GluI GCNGC 2 cut(s) 342, 1520
GsaI CCCAGC 1 cut(s) 1151
GsuI CTGGAG 3 cut(s) 551, 1442, 1493
HapII CCGG 2 cut(s) 607, 1526
HhaI GCGC 1 cut(s) 164
Hin1II CATG 7 cut(s) 44, 230, 247, 1069, 1106, 1121, 1481
Hin6I GCGC 1 cut(s) 162
HinP1I GCGC 1 cut(s) 162
HinfI GANTC 5 cut(s) 378, 809, 821, 1173, 1504
HpaII CCGG 2 cut(s) 607, 1526
HphI GGTGA 9 cut(s) 71, 308, 373, 419, 531, 539, 1426, 1506, 1513
Hpy166II GTNNAC 1 cut(s) 514
Hpy188I TCNGA 3 cut(s) 155, 1169, 1485
Hpy188III TCNNGA 8 cut(s) 249, 659, 688, 825, 908, 1103, 1472, 1526
Hpy8I GTNNAC 1 cut(s) 514
HpyAV CCTTC 3 cut(s) 133, 577, 1331
HpyCH4III ACNGT 2 cut(s) 131, 1576
HpyCH4IV ACGT 5 cut(s) 386, 655, 829, 890, 1202
HpyF10VI GCNNNNNNNGC 2 cut(s) 350, 1014
HpyF3I CTNAG 7 cut(s) 940, 951, 973, 1248, 1290, 1332, 1482
HpySE526I ACGT 5 cut(s) 386, 655, 829, 890, 1202
Hsp92II CATG 7 cut(s) 44, 230, 247, 1069, 1106, 1121, 1481
HspAI GCGC 1 cut(s) 162
Kpn2I TCCGGA 1 cut(s) 1525
Kzo9I GATC 5 cut(s) 88, 390, 754, 885, 910
LmnI GCTCC 4 cut(s) 4, 23, 1354, 1474
Lsp1109I GCAGC 1 cut(s) 1531
MaeI CTAG 1 cut(s) 47
MaeII ACGT 5 cut(s) 386, 655, 829, 890, 1202
MaeIII GTNAC 2 cut(s) 465, 1207
MalI GATC 5 cut(s) 90, 392, 756, 887, 912
MboI GATC 5 cut(s) 88, 390, 754, 885, 910
MfeI CAATTG 1 cut(s) 1224
MflI RGATCY 2 cut(s) 390, 754
MhlI GDGCHC 3 cut(s) 9, 701, 750
Mph1103I ATGCAT 2 cut(s) 644, 1199
MroI TCCGGA 1 cut(s) 1525
MseI TTAA 7 cut(s) 140, 417, 735, 759, 879, 1023, 1389
MslI CAYNNNNRTG 3 cut(s) 140, 155, 294
MspI CCGG 2 cut(s) 607, 1526
MunI CAATTG 1 cut(s) 1224
MvnI CGCG 1 cut(s) 1017
MwoI GCNNNNNNNGC 2 cut(s) 350, 1014
NdeII GATC 5 cut(s) 88, 390, 754, 885, 910
NlaIII CATG 7 cut(s) 44, 230, 247, 1069, 1106, 1121, 1481
NlaIV GGNNCC 2 cut(s) 6, 392
NmuCI GTSAC 1 cut(s) 1207
NsiI ATGCAT 2 cut(s) 644, 1199
NspI RCATGY 2 cut(s) 1121, 1481
NspV TTCGAA 3 cut(s) 376, 615, 812
PaeI GCATGC 1 cut(s) 1481
PagI TCATGA 1 cut(s) 1102
PciI ACATGT 1 cut(s) 1117
PfeI GAWTC 5 cut(s) 378, 809, 821, 1173, 1504
PflFI GACNNNGTC 2 cut(s) 831, 1142
PflMI CCANNNNNTGG 1 cut(s) 574
PinAI ACCGGT 1 cut(s) 606
PkrI GCNGC 2 cut(s) 343, 1521
PmaCI CACGTG 2 cut(s) 387, 891
PmlI CACGTG 2 cut(s) 387, 891
Ppu21I YACGTR 2 cut(s) 387, 891
PscI ACATGT 1 cut(s) 1117
Psp124BI GAGCTC 2 cut(s) 701, 750
PspCI CACGTG 2 cut(s) 387, 891
PspFI CCCAGC 1 cut(s) 1147
PspN4I GGNNCC 2 cut(s) 6, 392
PstI CTGCAG 1 cut(s) 1010
PsuI RGATCY 2 cut(s) 390, 754
PsyI GACNNNGTC 2 cut(s) 831, 1142
RsaI GTAC 1 cut(s) 281
RsaNI GTAC 1 cut(s) 280
RseI CAYNNNNRTG 3 cut(s) 140, 155, 294
SacI GAGCTC 2 cut(s) 701, 750
SaqAI TTAA 7 cut(s) 140, 417, 735, 759, 879, 1023, 1389
SatI GCNGC 2 cut(s) 342, 1520
Sau3AI GATC 5 cut(s) 88, 390, 754, 885, 910
SduI GDGCHC 3 cut(s) 9, 701, 750
SfcI CTRYAG 2 cut(s) 1006, 1311
SfuI TTCGAA 3 cut(s) 376, 615, 812
SmiMI CAYNNNNRTG 3 cut(s) 140, 155, 294
SmlI CTYRAG 1 cut(s) 517
SmoI CTYRAG 1 cut(s) 517
SphI GCATGC 1 cut(s) 1481
SsiI CCGC 2 cut(s) 342, 1517
SspI AATATT 5 cut(s) 714, 862, 1099, 1370, 1596
SspMI CTAG 1 cut(s) 47
SstI GAGCTC 2 cut(s) 701, 750
TaaI ACNGT 2 cut(s) 131, 1576
TaiI ACGT 5 cut(s) 389, 658, 832, 893, 1205
TaqI TCGA 7 cut(s) 75, 248, 336, 376, 615, 812, 1076
TauI GCSGC 1 cut(s) 344
TfiI GAWTC 5 cut(s) 378, 809, 821, 1173, 1504
Tru1I TTAA 7 cut(s) 140, 417, 735, 759, 879, 1023, 1389
Tru9I TTAA 7 cut(s) 140, 417, 735, 759, 879, 1023, 1389
TscAI CASTG 4 cut(s) 134, 171, 1573, 1581
TseFI GTSAC 1 cut(s) 1207
TseI GCWGC 1 cut(s) 1519
Tsp45I GTSAC 1 cut(s) 1207
TspGWI ACGGA 3 cut(s) 192, 515, 1299
TspRI CASTG 4 cut(s) 134, 171, 1573, 1581
Tth111I GACNNNGTC 2 cut(s) 831, 1142
Van91I CCANNNNNTGG 1 cut(s) 574
XapI RAATTY 1 cut(s) 434
XceI RCATGY 2 cut(s) 1121, 1481
XcmI CCANNNNNNNNNTGG 3 cut(s) 16, 1360, 1573
XspI CTAG 1 cut(s) 47
Zsp2I ATGCAT 2 cut(s) 644, 1199
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.