Rh5BG471300

LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0002877
Physical Location & Seq
Reverse (-)
75060716 .. 75068860
8145 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG471300.1

Sequence Viewer

Length: 1614 bp
ATGACAAGTTGTCAAGTTCAAGGAGCTTCTAGTGAGAGTATAATGATGGACAGATTCAGTGATCTCCCAGATGAAATTTCTCATCATATTATTTCGTTCCTCTCTGACTCTTTAAGGAACCTCATTCAAGTGGGAGCTGTGTCCAAAAGATGTAGGAAACTTTATCTGTCAGTTCCATCAATAACTATTGATGCAAGTAGCGTTGCAGATTGCACCAAGTTGCGAGTAAGATTGCTGAACTCTTGGGATAGGTACTTGTTTCACCGTGGTAATAATAAGATTGAGCACCTTAGTATTTACTGGGTTTTCAGAACCTCTTGGGAGGCAAAGATAAGTGATGACTATTTCCGAGTCACCACATGGATCCAAAATGCAGTGAGGTGTAATGTTGAAGTACTTCACCTTGATCTGAATCAATACGACCGCAGGATGACAGTTGTGTTGCCGTCTTGCATCTTCCTTTGTCATTCTTTGAGGACTCTATCTGTGAATTTGTATATGGGCAAGATTCAGTCACCCAACTTGTCTTTTTCCTGTAATCTCCACTACTTGAAGTTGAAGTATGTACGTCTTAAAGATGATGGTATTTTCAAATGGATCTCATATTCCTGCAAATGCATTAAGGAATTACATCTTGAAAAAGTTATTGGGATAAAAGATATCAAGATTGAGAGCTTGTCTTTGGAATCGTTTAGTCTTTTGCTTCCTTTTCAGAATTATGAGCTCTGGGATCTTAGCATTTCAGCTGAAAAACTTGAAGATATATGTATACACTGGCAATTTGATTCGAGACGTGATTCAAAGTTTCATCTTTTACCTCGCATCAGATCGTTTAATCTCTTTGCTCCAAAGCTTAAAAATTTCAAATGGAGTGGGAATTTTCTGAACCATCATCATCTTGGGAACTTGATGTGTTTAAAAGAAGCTGACATATTTCTGATGCCCGATTTAGATGATTCTAGCAACCTATTTGAGGTTCTTTGCAGCATATGTATGGTTAAAGTTCTAACCCTAAATGAAGAAAGTACAAAGGCTTTATGCAGGGGAGGTTCCATGCCGGTACCATTAGATAATATTCGTTACTTGAGTTTGCATGTTACAAGTCTGAATGCTGACTTGGTCGCGGCAATGGTGTCTATTTTTAGAGGAATTACTTCTTTGAATACTTTGTGCATAATGTCTGACCTATCCAAGTCATCTGTATCTGGAACTGAAGATGACAGTGATATTGAGGAAGTGGTTGAGGATAATGATACGGAGGAAGAGAATAATGAAAACGAGGAAGAGGACAATGACAATTATACCGAGGAAGAAGATAATGAAACTAAGGAGGAAAATGCAACTGAGAAAGAGAATAATGAATCCAGGTTCAATGTGGAATACTGGAAATCCCAAAACCTAACCTTTATTTATCAGCTCAAGGAAGTGAGAATAGACCTTCTAGATGGTAATAATGAATTGGAGTTCGCAAGGTATATTCTGGAGAATGCTCCGAGTTTGGAGAAAATGGTCATAATTCAATCACCCCAGCATTCGGATGTTGTGGAGATGGTAACCAAAAGCAAGATGATTTCCAATGCGACAGTTGTTTTACAAGAAAGGCAGAGCAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like FBD

Protein Analysis

537

Amino Acids

62.07

Weight (kDa)

5.15

Isoelectric Point (pI)

46.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At5g56370 PF24758 119 - 264 9.5e-07 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1060
AccB1I GGYRCC 1 cut(s) 1060
AccI GTMKAC 1 cut(s) 769
AccII CGCG 1 cut(s) 1124
AciI CCGC 2 cut(s) 424, 1124
AclWI GGATC 4 cut(s) 358, 371, 605, 738
AcsI RAATTY 4 cut(s) 75, 490, 859, 877
AcuI CTGAAG 1 cut(s) 1233
AfaI GTAC 5 cut(s) 254, 396, 567, 1027, 1062
AfiI CCNNNNNNNGG 2 cut(s) 973, 1534
AhdI GACNNNNNGTC 1 cut(s) 9
AjiI CACGTC 1 cut(s) 794
AjnI CCWGG 1 cut(s) 1364
AjuI GAANNNNNNNTTGG 4 cut(s) 630, 662, 1100, 1132
AluBI AGCT 8 cut(s) 26, 137, 675, 724, 746, 853, 926, 1417
AluI AGCT 8 cut(s) 26, 137, 675, 724, 746, 853, 926, 1417
Alw21I GWGCWC 2 cut(s) 288, 726
Alw26I GTCTC 1 cut(s) 784
AlwI GGATC 4 cut(s) 358, 371, 605, 738
ApeKI GCWGC 1 cut(s) 984
ApoI RAATTY 4 cut(s) 75, 490, 859, 877
Asp700I GAANNNNTTC 2 cut(s) 396, 1153
Asp718I GGTACC 1 cut(s) 1060
AsuHPI GGTGA 5 cut(s) 254, 346, 392, 507, 1515
BamHI GGATCC 1 cut(s) 363
BanI GGYRCC 1 cut(s) 1060
BanII GRGCYC 1 cut(s) 726
Bbv12I GWGCWC 2 cut(s) 288, 726
BbvI GCAGC 1 cut(s) 996
BccI CCATC 6 cut(s) 40, 184, 575, 897, 1439, 1543
BceAI ACGGC 1 cut(s) 430
BciT130I CCWGG 1 cut(s) 1366
BcoDI GTCTC 1 cut(s) 784
BfaI CTAG 3 cut(s) 30, 960, 1442
BisI GCNGC 2 cut(s) 985, 1125
BlsI GCNGC 2 cut(s) 986, 1126
BmcAI AGTACT 1 cut(s) 396
Bme1390I CCNGG 1 cut(s) 1366
BmeRI GACNNNNNGTC 1 cut(s) 9
BmgBI CACGTC 1 cut(s) 794
BmiI GGNNCC 4 cut(s) 119, 365, 1051, 1062
BmrFI CCNGG 1 cut(s) 1366
BmrI ACTGGG 1 cut(s) 310
BmsI GCATC 4 cut(s) 181, 462, 831, 930
BmuI ACTGGG 1 cut(s) 310
BpmI CTGGAG 1 cut(s) 1502
BpuEI CTTGAG 2 cut(s) 1105, 1403
BsaBI GATNNNNATC 1 cut(s) 411
BsaJI CCNNGG 2 cut(s) 265, 1305
Bsc4I CCNNNNNNNGG 2 cut(s) 973, 1534
Bse118I RCCGGY 1 cut(s) 1057
Bse1I ACTGG 3 cut(s) 305, 779, 1388
Bse3DI GCAATG 1 cut(s) 1134
Bse8I GATNNNNATC 1 cut(s) 411
BseBI CCWGG 1 cut(s) 1366
BseDI CCNNGG 2 cut(s) 265, 1305
BseGI GGATG 2 cut(s) 435, 1543
BseJI GATNNNNATC 1 cut(s) 411
BseLI CCNNNNNNNGG 2 cut(s) 973, 1534
BseMI GCAATG 1 cut(s) 1134
BseMII CTCAG 1 cut(s) 1335
BseNI ACTGG 3 cut(s) 305, 779, 1388
BseXI GCAGC 1 cut(s) 996
BseYI CCCAGC 1 cut(s) 1527
Bsh1236I CGCG 1 cut(s) 1124
Bsh1285I CGRYCG 1 cut(s) 424
BshNI GGYRCC 1 cut(s) 1060
BsiEI CGRYCG 1 cut(s) 424
BsiHKAI GWGCWC 2 cut(s) 288, 726
BsiSI CCGG 1 cut(s) 1058
BslI CCNNNNNNNGG 2 cut(s) 973, 1534
BsmAI GTCTC 1 cut(s) 784
BsmBI CGTCTC 1 cut(s) 784
BsmI GAATGC 3 cut(s) 1114, 1492, 1531
Bsp1286I GDGCHC 2 cut(s) 288, 726
Bsp143I GATC 6 cut(s) 61, 363, 406, 597, 730, 827
BspACI CCGC 2 cut(s) 424, 1124
BspCNI CTCAG 1 cut(s) 1336
BspFNI CGCG 1 cut(s) 1124
BspLI GGNNCC 4 cut(s) 119, 365, 1051, 1062
BspPI GGATC 4 cut(s) 358, 371, 605, 738
BspT107I GGYRCC 1 cut(s) 1060
BsrDI GCAATG 1 cut(s) 1134
BsrFI RCCGGY 1 cut(s) 1057
BsrI ACTGG 3 cut(s) 305, 779, 1388
BssAI RCCGGY 1 cut(s) 1057
BssECI CCNNGG 2 cut(s) 265, 1305
BssMI GATC 6 cut(s) 61, 363, 406, 597, 730, 827
BssNAI GTATAC 1 cut(s) 770
Bst1107I GTATAC 1 cut(s) 770
Bst2UI CCWGG 1 cut(s) 1366
Bst4CI ACNGT 4 cut(s) 266, 436, 1223, 1585
Bst6I CTCTTC 2 cut(s) 1257, 1278
BstDEI CTNAG 4 cut(s) 290, 734, 1326, 1344
BstDSI CCRYGG 1 cut(s) 265
BstEII GGTNACC 1 cut(s) 1552
BstENI CCTNNNNNAGG 1 cut(s) 971
BstF5I GGATG 2 cut(s) 435, 1543
BstFNI CGCG 1 cut(s) 1124
BstKTI GATC 6 cut(s) 64, 366, 409, 600, 733, 830
BstMAI GTCTC 1 cut(s) 784
BstMBI GATC 6 cut(s) 61, 363, 406, 597, 730, 827
BstMCI CGRYCG 1 cut(s) 424
BstNI CCWGG 1 cut(s) 1366
BstNSI RCATGY 1 cut(s) 1097
BstPI GGTNACC 1 cut(s) 1552
BstSCI CCNGG 1 cut(s) 1364
BstUI CGCG 1 cut(s) 1124
BstV1I GCAGC 1 cut(s) 996
BstX2I RGATCY 3 cut(s) 363, 597, 730
BstYI RGATCY 3 cut(s) 363, 597, 730
BstZ17I GTATAC 1 cut(s) 770
BtgI CCRYGG 1 cut(s) 265
BtrI CACGTC 1 cut(s) 794
BtsCI GGATG 2 cut(s) 435, 1543
BtsI GCAGTG 1 cut(s) 381
BtsIMutI CAGTG 4 cut(s) 64, 381, 772, 1228
Cfr10I RCCGGY 1 cut(s) 1057
Csp6I GTAC 5 cut(s) 253, 395, 566, 1026, 1061
CspCI CAANNNNNGTGG 2 cut(s) 853, 888
CviAII CATG 3 cut(s) 360, 1054, 1094
CviJI RGCY 9 cut(s) 26, 137, 675, 724, 746, 853, 926, 1034, 1417
CviKI_1 RGCY 9 cut(s) 26, 137, 675, 724, 746, 853, 926, 1034, 1417
CviQI GTAC 5 cut(s) 253, 395, 566, 1026, 1061
DdeI CTNAG 4 cut(s) 290, 734, 1326, 1344
DpnI GATC 6 cut(s) 63, 365, 408, 599, 732, 829
DpnII GATC 6 cut(s) 61, 363, 406, 597, 730, 827
DraI TTTAAA 1 cut(s) 918
DriI GACNNNNNGTC 1 cut(s) 9
Eam1104I CTCTTC 2 cut(s) 1257, 1278
Eam1105I GACNNNNNGTC 1 cut(s) 9
EarI CTCTTC 2 cut(s) 1257, 1278
Ecl136II GAGCTC 1 cut(s) 724
Eco24I GRGCYC 1 cut(s) 726
Eco32I GATATC 1 cut(s) 661
Eco53kI GAGCTC 1 cut(s) 724
Eco57I CTGAAG 1 cut(s) 1233
Eco91I GGTNACC 1 cut(s) 1552
EcoICRI GAGCTC 1 cut(s) 724
EcoNI CCTNNNNNAGG 1 cut(s) 971
EcoO65I GGTNACC 1 cut(s) 1552
EcoRII CCWGG 1 cut(s) 1364
EcoRV GATATC 1 cut(s) 661
EcoT22I ATGCAT 1 cut(s) 620
EcoT38I GRGCYC 1 cut(s) 726
Esp3I CGTCTC 1 cut(s) 784
FaeI CATG 3 cut(s) 363, 1057, 1097
FatI CATG 3 cut(s) 359, 1053, 1093
FauNDI CATATG 1 cut(s) 989
FblI GTMKAC 1 cut(s) 769
Fnu4HI GCNGC 2 cut(s) 985, 1125
FokI GGATG 2 cut(s) 442, 1550
FriOI GRGCYC 1 cut(s) 726
Fsp4HI GCNGC 2 cut(s) 985, 1125
FspBI CTAG 3 cut(s) 30, 960, 1442
GluI GCNGC 2 cut(s) 985, 1125
GsaI CCCAGC 1 cut(s) 1531
GsuI CTGGAG 1 cut(s) 1502
HapII CCGG 1 cut(s) 1058
Hin1II CATG 3 cut(s) 363, 1057, 1097
HindIII AAGCTT 1 cut(s) 851
HpaII CCGG 1 cut(s) 1058
HphI GGTGA 5 cut(s) 254, 346, 392, 507, 1515
Hpy166II GTNNAC 1 cut(s) 770
Hpy188III TCNNGA 6 cut(s) 635, 664, 789, 1206, 1442, 1481
Hpy8I GTNNAC 1 cut(s) 770
HpyAV CCTTC 1 cut(s) 1448
HpyCH4III ACNGT 4 cut(s) 266, 436, 1223, 1585
HpyCH4IV ACGT 2 cut(s) 568, 793
HpyF3I CTNAG 4 cut(s) 290, 734, 1326, 1344
HpySE526I ACGT 2 cut(s) 568, 793
Hsp92II CATG 3 cut(s) 363, 1057, 1097
KpnI GGTACC 1 cut(s) 1064
Kzo9I GATC 6 cut(s) 61, 363, 406, 597, 730, 827
LmnI GCTCC 4 cut(s) 23, 134, 850, 1495
Lsp1109I GCAGC 1 cut(s) 996
LweI GCATC 4 cut(s) 181, 462, 831, 930
MaeI CTAG 3 cut(s) 30, 960, 1442
MaeII ACGT 2 cut(s) 568, 793
MaeIII GTNAC 5 cut(s) 352, 513, 1079, 1096, 1552
MalI GATC 6 cut(s) 63, 365, 408, 599, 732, 829
MboI GATC 6 cut(s) 61, 363, 406, 597, 730, 827
MboII GAAGA 8 cut(s) 448, 770, 1031, 1226, 1274, 1295, 1322, 1325
MflI RGATCY 3 cut(s) 363, 597, 730
MhlI GDGCHC 2 cut(s) 288, 726
MlyI GAGTC 3 cut(s) 101, 360, 472
Mph1103I ATGCAT 1 cut(s) 620
MroXI GAANNNNTTC 2 cut(s) 396, 1153
MseI TTAA 7 cut(s) 113, 573, 621, 834, 855, 917, 999
MslI CAYNNNNRTG 3 cut(s) 128, 992, 1536
MspA1I CMGCKG 1 cut(s) 746
MspI CCGG 1 cut(s) 1058
MspR9I CCNGG 1 cut(s) 1366
Mva1269I GAATGC 3 cut(s) 1114, 1492, 1531
MvaI CCWGG 1 cut(s) 1366
MvnI CGCG 1 cut(s) 1124
NdeI CATATG 1 cut(s) 989
NdeII GATC 6 cut(s) 61, 363, 406, 597, 730, 827
NlaIII CATG 3 cut(s) 363, 1057, 1097
NlaIV GGNNCC 4 cut(s) 119, 365, 1051, 1062
NmuCI GTSAC 2 cut(s) 352, 513
NsiI ATGCAT 1 cut(s) 620
NspI RCATGY 1 cut(s) 1097
PctI GAATGC 3 cut(s) 1114, 1492, 1531
PdmI GAANNNNTTC 2 cut(s) 396, 1153
PfeI GAWTC 8 cut(s) 54, 412, 508, 686, 785, 797, 956, 1361
PflFI GACNNNGTC 1 cut(s) 1118
PkrI GCNGC 2 cut(s) 986, 1126
PleI GAGTC 3 cut(s) 101, 359, 472
PpsI GAGTC 3 cut(s) 101, 359, 472
Psp124BI GAGCTC 1 cut(s) 726
Psp6I CCWGG 1 cut(s) 1364
PspEI GGTNACC 1 cut(s) 1552
PspFI CCCAGC 1 cut(s) 1527
PspGI CCWGG 1 cut(s) 1364
PspN4I GGNNCC 4 cut(s) 119, 365, 1051, 1062
PsuI RGATCY 3 cut(s) 363, 597, 730
PsyI GACNNNGTC 1 cut(s) 1118
PvuII CAGCTG 1 cut(s) 746
RsaI GTAC 5 cut(s) 254, 396, 567, 1027, 1062
RsaNI GTAC 5 cut(s) 253, 395, 566, 1026, 1061
RseI CAYNNNNRTG 3 cut(s) 128, 992, 1536
SacI GAGCTC 1 cut(s) 726
SaqAI TTAA 7 cut(s) 113, 573, 621, 834, 855, 917, 999
SatI GCNGC 2 cut(s) 985, 1125
Sau3AI GATC 6 cut(s) 61, 363, 406, 597, 730, 827
ScaI AGTACT 1 cut(s) 396
SchI GAGTC 3 cut(s) 101, 360, 472
ScrFI CCNGG 1 cut(s) 1366
SduI GDGCHC 2 cut(s) 288, 726
SfaNI GCATC 4 cut(s) 181, 462, 831, 930
SmiMI CAYNNNNRTG 3 cut(s) 128, 992, 1536
SmlI CTYRAG 2 cut(s) 1084, 1418
SmoI CTYRAG 2 cut(s) 1084, 1418
SsiI CCGC 2 cut(s) 424, 1124
SspI AATATT 1 cut(s) 1075
SspMI CTAG 3 cut(s) 30, 960, 1442
SstI GAGCTC 1 cut(s) 726
StyD4I CCNGG 1 cut(s) 1364
TaaI ACNGT 4 cut(s) 266, 436, 1223, 1585
TaiI ACGT 2 cut(s) 571, 796
TaqI TCGA 1 cut(s) 788
TatI WGTACW 2 cut(s) 394, 1025
TauI GCSGC 1 cut(s) 1127
TfiI GAWTC 8 cut(s) 54, 412, 508, 686, 785, 797, 956, 1361
Tru1I TTAA 7 cut(s) 113, 573, 621, 834, 855, 917, 999
Tru9I TTAA 7 cut(s) 113, 573, 621, 834, 855, 917, 999
TscAI CASTG 4 cut(s) 64, 381, 779, 1228
TseFI GTSAC 2 cut(s) 352, 513
TseI GCWGC 1 cut(s) 984
Tsp45I GTSAC 2 cut(s) 352, 513
TspDTI ATGAA 7 cut(s) 87, 797, 1032, 1287, 1335, 1374, 1470
TspGWI ACGGA 1 cut(s) 1271
TspRI CASTG 4 cut(s) 64, 381, 779, 1228
Tth111I GACNNNGTC 1 cut(s) 1118
XagI CCTNNNNNAGG 1 cut(s) 971
XapI RAATTY 4 cut(s) 75, 490, 859, 877
XbaI TCTAGA 1 cut(s) 1441
XceI RCATGY 1 cut(s) 1097
XcmI CCANNNNNNNNNTGG 2 cut(s) 896, 1372
XmiI GTMKAC 1 cut(s) 769
XmnI GAANNNNTTC 2 cut(s) 396, 1153
XspI CTAG 3 cut(s) 30, 960, 1442
ZrmI AGTACT 1 cut(s) 396
Zsp2I ATGCAT 1 cut(s) 620
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.