Rh2DG545800

LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0002877
Physical Location & Seq
Forward (+)
77487269 .. 77490482
3214 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG545800.1

Sequence Viewer

Length: 1611 bp
ATGACAAGTTGTCAAGTTCAAGGAGCTTCTAGTGAGAGTATAATGATGGACAGATTCAGTGATCTCCCAGATGAAATTTCTCATCATATTATTTCGTTCCTCTCTGACTCTTTAAGGAACCTCATTCAAGTGGGAGCTGTGTCCAAAAGATGTAGGAAACTTTATCTGTCAGTTCCATCAATAATTATTGATGCAAGTAGCGTTGCAGATTGCACCAAGTTGCGAGTAAGATTGTTGAACTCTTGGGATAGGTACTTGTTTCACCGTGGTAATAATAAGATTGAGCGCCTTAGTATTTACTGGGTTTTCAGAACCTCTTGGGAGGCAAAGATAAGTGATGAGTATTTCCGAGTCACCACATGGATCCAAAATGCAGTGAGGTGTAATGTTGAAGTACTTCACCTTGATCTGAATCAATACGACCTCAGGATGACAGTTGTGTTGCCGTCTTGCATCTTCCTTTGTCGTTCTTTGAGGACTCTATCTGTGAATTTGTATATGGGCAAGATTCAGTCACCCAACTTGTCTTTTTCCTGTAATCTCCACTACTTGAAGTTGAAGTATGTACGTCTTAAAGATGACTGTATTTTCAAATGGATCTCATATTCCTGCAAATGCATAAAGGAATTACATCTTGAAAAAGTTATTGGGATAAAAGATATCAAGATTGAGAGCTCGTCTTTGGAATCGTTTAGTCTTTTGCTTCCTTTTCAGAATTATGAGCTCTGGGATCTTAGCATTTCAGCTGAAAAACTTGAAGATATATGTATACACTGGCAATTTGATTCGAGACGTGATTCAAAGTTTCATCTTTTACCTCGCATCAGATCATTTAATCTCTTTGCTCCAAAGCTTAAAAATTTCAAATGGAGTGGGAATTTTCTGAACCATCATCATCTTGGGAACTTGATGTGTTTAGAAGAAGCTGACATATTTCTGATGCCCGATTTAGATGATTCTAGCAACCTATTTGAGGTTCTTTGCAGCATATGTATGGTTAAAGTTCTAACCCTAAATGAAGAAACTACAGAGGCTTTATGCAGGGGAGGTTCCATGCCGGTACCATTAGATAGTATTCGTTACTTGAGTTTGCATGTTACAAGTCTGAATGCTGACTTGGTCGCGGCAATGGTGTCTATTTTTAGAGGAATTACTTCTTTGAATACTTTGTGCATAATGTCTGACCTATCCAAGTCATCTGTATCTGGAACTGAAGATGACAGTGATATTGAGGAAGTGGTTGAGGATAATGATACGGAGGAAGAGGATAATGAAAACGAGGAAGAGGACAATGACAATTATACCGAGGAAGAAGATAATGAAACTGAGGAAAATGCAACTGAGAAAGAGAATAATGAATCCAGGTTCAATGTGGAATACTGGAAATCCCAAAACCTAACCTTTATTTATCAGCTCAAGGAAGTGAGAATAGACCTTCTAGATGGTAATAATGAATTGGAGTTCGCAAGGTATATTCTGGAGAATGCTCCGAGTTTGGAGAAAATGGTCATAATTCAATCACCCCAGCATTCGGATGTTGTGGAGATGGTAACCAAAAGCAAGATGATTTCCAATGCGACAGTTGTTTTACAAGAAAGGCAGAGCAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like FBD

Protein Analysis

536

Amino Acids

61.97

Weight (kDa)

4.93

Isoelectric Point (pI)

47.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At5g56370 PF24758 119 - 263 3e-07 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1060
AccB1I GGYRCC 1 cut(s) 1060
AccI GTMKAC 1 cut(s) 769
AccII CGCG 1 cut(s) 1124
AciI CCGC 1 cut(s) 1124
AclWI GGATC 4 cut(s) 358, 371, 605, 738
AcsI RAATTY 4 cut(s) 75, 490, 859, 877
AcuI CTGAAG 1 cut(s) 1233
AfaI GTAC 4 cut(s) 254, 396, 567, 1062
AfiI CCNNNNNNNGG 2 cut(s) 973, 1531
AhdI GACNNNNNGTC 1 cut(s) 9
AjiI CACGTC 1 cut(s) 794
AjnI CCWGG 1 cut(s) 1361
AjuI GAANNNNNNNTTGG 4 cut(s) 630, 662, 1100, 1132
AluBI AGCT 8 cut(s) 26, 137, 675, 724, 746, 853, 926, 1414
AluI AGCT 8 cut(s) 26, 137, 675, 724, 746, 853, 926, 1414
Alw21I GWGCWC 2 cut(s) 677, 726
Alw26I GTCTC 1 cut(s) 784
AlwI GGATC 4 cut(s) 358, 371, 605, 738
ApeKI GCWGC 1 cut(s) 984
ApoI RAATTY 4 cut(s) 75, 490, 859, 877
Asp700I GAANNNNTTC 2 cut(s) 396, 1153
Asp718I GGTACC 1 cut(s) 1060
AspLEI GCGC 1 cut(s) 288
AsuHPI GGTGA 5 cut(s) 254, 346, 392, 507, 1512
AxyI CCTNAGG 1 cut(s) 425
BamHI GGATCC 1 cut(s) 363
BanI GGYRCC 1 cut(s) 1060
BanII GRGCYC 2 cut(s) 677, 726
Bbv12I GWGCWC 2 cut(s) 677, 726
BbvI GCAGC 1 cut(s) 996
BccI CCATC 5 cut(s) 40, 184, 897, 1436, 1540
BceAI ACGGC 1 cut(s) 430
BciT130I CCWGG 1 cut(s) 1363
BcoDI GTCTC 1 cut(s) 784
BfaI CTAG 3 cut(s) 30, 960, 1439
BfmI CTRYAG 1 cut(s) 1026
BfoI RGCGCY 1 cut(s) 289
BisI GCNGC 2 cut(s) 985, 1125
BlsI GCNGC 2 cut(s) 986, 1126
BmcAI AGTACT 1 cut(s) 396
Bme1390I CCNGG 1 cut(s) 1363
BmeRI GACNNNNNGTC 1 cut(s) 9
BmgBI CACGTC 1 cut(s) 794
BmiI GGNNCC 4 cut(s) 119, 365, 1051, 1062
BmrFI CCNGG 1 cut(s) 1363
BmrI ACTGGG 1 cut(s) 310
BmsI GCATC 4 cut(s) 181, 462, 831, 930
BmuI ACTGGG 1 cut(s) 310
BpmI CTGGAG 1 cut(s) 1499
BpuEI CTTGAG 2 cut(s) 1105, 1400
BsaBI GATNNNNATC 1 cut(s) 411
BsaJI CCNNGG 2 cut(s) 265, 1305
Bsc4I CCNNNNNNNGG 2 cut(s) 973, 1531
Bse118I RCCGGY 1 cut(s) 1057
Bse1I ACTGG 3 cut(s) 305, 779, 1385
Bse21I CCTNAGG 1 cut(s) 425
Bse3DI GCAATG 1 cut(s) 1134
Bse8I GATNNNNATC 1 cut(s) 411
BseBI CCWGG 1 cut(s) 1363
BseDI CCNNGG 2 cut(s) 265, 1305
BseGI GGATG 2 cut(s) 435, 1540
BseJI GATNNNNATC 1 cut(s) 411
BseLI CCNNNNNNNGG 2 cut(s) 973, 1531
BseMI GCAATG 1 cut(s) 1134
BseMII CTCAG 3 cut(s) 439, 1317, 1332
BseNI ACTGG 3 cut(s) 305, 779, 1385
BseXI GCAGC 1 cut(s) 996
BseYI CCCAGC 1 cut(s) 1524
Bsh1236I CGCG 1 cut(s) 1124
BshNI GGYRCC 1 cut(s) 1060
BsiHKAI GWGCWC 2 cut(s) 677, 726
BsiSI CCGG 1 cut(s) 1058
BslI CCNNNNNNNGG 2 cut(s) 973, 1531
BsmAI GTCTC 1 cut(s) 784
BsmBI CGTCTC 1 cut(s) 784
BsmI GAATGC 3 cut(s) 1114, 1489, 1528
Bsp1286I GDGCHC 2 cut(s) 677, 726
Bsp143I GATC 6 cut(s) 61, 363, 406, 597, 730, 827
BspACI CCGC 1 cut(s) 1124
BspCNI CTCAG 3 cut(s) 438, 1318, 1333
BspFNI CGCG 1 cut(s) 1124
BspLI GGNNCC 4 cut(s) 119, 365, 1051, 1062
BspPI GGATC 4 cut(s) 358, 371, 605, 738
BspT107I GGYRCC 1 cut(s) 1060
BsrDI GCAATG 1 cut(s) 1134
BsrFI RCCGGY 1 cut(s) 1057
BsrI ACTGG 3 cut(s) 305, 779, 1385
BssAI RCCGGY 1 cut(s) 1057
BssECI CCNNGG 2 cut(s) 265, 1305
BssMI GATC 6 cut(s) 61, 363, 406, 597, 730, 827
BssNAI GTATAC 1 cut(s) 770
Bst1107I GTATAC 1 cut(s) 770
Bst2UI CCWGG 1 cut(s) 1363
Bst4CI ACNGT 5 cut(s) 266, 436, 584, 1223, 1582
Bst6I CTCTTC 2 cut(s) 1257, 1278
BstDEI CTNAG 5 cut(s) 290, 425, 734, 1326, 1341
BstDSI CCRYGG 1 cut(s) 265
BstEII GGTNACC 1 cut(s) 1549
BstENI CCTNNNNNAGG 1 cut(s) 971
BstF5I GGATG 2 cut(s) 435, 1540
BstFNI CGCG 1 cut(s) 1124
BstH2I RGCGCY 1 cut(s) 289
BstHHI GCGC 1 cut(s) 288
BstKTI GATC 6 cut(s) 64, 366, 409, 600, 733, 830
BstMAI GTCTC 1 cut(s) 784
BstMBI GATC 6 cut(s) 61, 363, 406, 597, 730, 827
BstNI CCWGG 1 cut(s) 1363
BstNSI RCATGY 1 cut(s) 1097
BstPI GGTNACC 1 cut(s) 1549
BstSCI CCNGG 1 cut(s) 1361
BstSFI CTRYAG 1 cut(s) 1026
BstUI CGCG 1 cut(s) 1124
BstV1I GCAGC 1 cut(s) 996
BstX2I RGATCY 3 cut(s) 363, 597, 730
BstYI RGATCY 3 cut(s) 363, 597, 730
BstZ17I GTATAC 1 cut(s) 770
Bsu36I CCTNAGG 1 cut(s) 425
BtgI CCRYGG 1 cut(s) 265
BtrI CACGTC 1 cut(s) 794
BtsCI GGATG 2 cut(s) 435, 1540
BtsI GCAGTG 1 cut(s) 381
BtsIMutI CAGTG 4 cut(s) 64, 381, 772, 1228
CfoI GCGC 1 cut(s) 288
Cfr10I RCCGGY 1 cut(s) 1057
Csp6I GTAC 4 cut(s) 253, 395, 566, 1061
CspCI CAANNNNNGTGG 2 cut(s) 853, 888
CviAII CATG 3 cut(s) 360, 1054, 1094
CviJI RGCY 9 cut(s) 26, 137, 675, 724, 746, 853, 926, 1034, 1414
CviKI_1 RGCY 9 cut(s) 26, 137, 675, 724, 746, 853, 926, 1034, 1414
CviQI GTAC 4 cut(s) 253, 395, 566, 1061
DdeI CTNAG 5 cut(s) 290, 425, 734, 1326, 1341
DpnI GATC 6 cut(s) 63, 365, 408, 599, 732, 829
DpnII GATC 6 cut(s) 61, 363, 406, 597, 730, 827
DriI GACNNNNNGTC 1 cut(s) 9
Eam1104I CTCTTC 2 cut(s) 1257, 1278
Eam1105I GACNNNNNGTC 1 cut(s) 9
EarI CTCTTC 2 cut(s) 1257, 1278
Ecl136II GAGCTC 2 cut(s) 675, 724
Eco24I GRGCYC 2 cut(s) 677, 726
Eco32I GATATC 1 cut(s) 661
Eco53kI GAGCTC 2 cut(s) 675, 724
Eco57I CTGAAG 1 cut(s) 1233
Eco81I CCTNAGG 1 cut(s) 425
Eco91I GGTNACC 1 cut(s) 1549
EcoICRI GAGCTC 2 cut(s) 675, 724
EcoNI CCTNNNNNAGG 1 cut(s) 971
EcoO65I GGTNACC 1 cut(s) 1549
EcoRII CCWGG 1 cut(s) 1361
EcoRV GATATC 1 cut(s) 661
EcoT22I ATGCAT 1 cut(s) 620
EcoT38I GRGCYC 2 cut(s) 677, 726
Esp3I CGTCTC 1 cut(s) 784
FaeI CATG 3 cut(s) 363, 1057, 1097
FatI CATG 3 cut(s) 359, 1053, 1093
FauNDI CATATG 1 cut(s) 989
FblI GTMKAC 1 cut(s) 769
Fnu4HI GCNGC 2 cut(s) 985, 1125
FokI GGATG 2 cut(s) 442, 1547
FriOI GRGCYC 2 cut(s) 677, 726
Fsp4HI GCNGC 2 cut(s) 985, 1125
FspBI CTAG 3 cut(s) 30, 960, 1439
GlaI GCGC 1 cut(s) 287
GluI GCNGC 2 cut(s) 985, 1125
GsaI CCCAGC 1 cut(s) 1528
GsuI CTGGAG 1 cut(s) 1499
HaeII RGCGCY 1 cut(s) 289
HapII CCGG 1 cut(s) 1058
HhaI GCGC 1 cut(s) 288
Hin1II CATG 3 cut(s) 363, 1057, 1097
Hin6I GCGC 1 cut(s) 286
HinP1I GCGC 1 cut(s) 286
HindIII AAGCTT 1 cut(s) 851
HpaII CCGG 1 cut(s) 1058
HphI GGTGA 5 cut(s) 254, 346, 392, 507, 1512
Hpy166II GTNNAC 1 cut(s) 770
Hpy188III TCNNGA 7 cut(s) 427, 635, 664, 789, 1206, 1439, 1478
Hpy8I GTNNAC 1 cut(s) 770
HpyAV CCTTC 1 cut(s) 1445
HpyCH4III ACNGT 5 cut(s) 266, 436, 584, 1223, 1582
HpyCH4IV ACGT 2 cut(s) 568, 793
HpyF3I CTNAG 5 cut(s) 290, 425, 734, 1326, 1341
HpySE526I ACGT 2 cut(s) 568, 793
Hsp92II CATG 3 cut(s) 363, 1057, 1097
HspAI GCGC 1 cut(s) 286
KpnI GGTACC 1 cut(s) 1064
Kzo9I GATC 6 cut(s) 61, 363, 406, 597, 730, 827
LmnI GCTCC 4 cut(s) 23, 134, 850, 1492
Lsp1109I GCAGC 1 cut(s) 996
LweI GCATC 4 cut(s) 181, 462, 831, 930
MaeI CTAG 3 cut(s) 30, 960, 1439
MaeII ACGT 2 cut(s) 568, 793
MaeIII GTNAC 5 cut(s) 352, 513, 1079, 1096, 1549
MalI GATC 6 cut(s) 63, 365, 408, 599, 732, 829
MboI GATC 6 cut(s) 61, 363, 406, 597, 730, 827
MboII GAAGA 9 cut(s) 448, 770, 932, 1031, 1226, 1274, 1295, 1322, 1325
MflI RGATCY 3 cut(s) 363, 597, 730
MhlI GDGCHC 2 cut(s) 677, 726
MlyI GAGTC 3 cut(s) 101, 360, 472
Mph1103I ATGCAT 1 cut(s) 620
MroXI GAANNNNTTC 2 cut(s) 396, 1153
MseI TTAA 5 cut(s) 113, 573, 834, 855, 999
MslI CAYNNNNRTG 3 cut(s) 128, 992, 1533
MspA1I CMGCKG 1 cut(s) 746
MspI CCGG 1 cut(s) 1058
MspR9I CCNGG 1 cut(s) 1363
Mva1269I GAATGC 3 cut(s) 1114, 1489, 1528
MvaI CCWGG 1 cut(s) 1363
MvnI CGCG 1 cut(s) 1124
NdeI CATATG 1 cut(s) 989
NdeII GATC 6 cut(s) 61, 363, 406, 597, 730, 827
NlaIII CATG 3 cut(s) 363, 1057, 1097
NlaIV GGNNCC 4 cut(s) 119, 365, 1051, 1062
NmuCI GTSAC 2 cut(s) 352, 513
NsiI ATGCAT 1 cut(s) 620
NspI RCATGY 1 cut(s) 1097
PctI GAATGC 3 cut(s) 1114, 1489, 1528
PdmI GAANNNNTTC 2 cut(s) 396, 1153
PfeI GAWTC 8 cut(s) 54, 412, 508, 686, 785, 797, 956, 1358
PflFI GACNNNGTC 1 cut(s) 1118
PkrI GCNGC 2 cut(s) 986, 1126
PleI GAGTC 3 cut(s) 101, 359, 472
PpsI GAGTC 3 cut(s) 101, 359, 472
Psp124BI GAGCTC 2 cut(s) 677, 726
Psp6I CCWGG 1 cut(s) 1361
PspEI GGTNACC 1 cut(s) 1549
PspFI CCCAGC 1 cut(s) 1524
PspGI CCWGG 1 cut(s) 1361
PspN4I GGNNCC 4 cut(s) 119, 365, 1051, 1062
PsuI RGATCY 3 cut(s) 363, 597, 730
PsyI GACNNNGTC 1 cut(s) 1118
PvuII CAGCTG 1 cut(s) 746
RsaI GTAC 4 cut(s) 254, 396, 567, 1062
RsaNI GTAC 4 cut(s) 253, 395, 566, 1061
RseI CAYNNNNRTG 3 cut(s) 128, 992, 1533
SacI GAGCTC 2 cut(s) 677, 726
SaqAI TTAA 5 cut(s) 113, 573, 834, 855, 999
SatI GCNGC 2 cut(s) 985, 1125
Sau3AI GATC 6 cut(s) 61, 363, 406, 597, 730, 827
ScaI AGTACT 1 cut(s) 396
SchI GAGTC 3 cut(s) 101, 360, 472
ScrFI CCNGG 1 cut(s) 1363
SduI GDGCHC 2 cut(s) 677, 726
SfaNI GCATC 4 cut(s) 181, 462, 831, 930
SfcI CTRYAG 1 cut(s) 1026
SmiMI CAYNNNNRTG 3 cut(s) 128, 992, 1533
SmlI CTYRAG 2 cut(s) 1084, 1415
SmoI CTYRAG 2 cut(s) 1084, 1415
SsiI CCGC 1 cut(s) 1124
SspMI CTAG 3 cut(s) 30, 960, 1439
SstI GAGCTC 2 cut(s) 677, 726
StyD4I CCNGG 1 cut(s) 1361
TaaI ACNGT 5 cut(s) 266, 436, 584, 1223, 1582
TaiI ACGT 2 cut(s) 571, 796
TaqI TCGA 1 cut(s) 788
TatI WGTACW 1 cut(s) 394
TauI GCSGC 1 cut(s) 1127
TfiI GAWTC 8 cut(s) 54, 412, 508, 686, 785, 797, 956, 1358
Tru1I TTAA 5 cut(s) 113, 573, 834, 855, 999
Tru9I TTAA 5 cut(s) 113, 573, 834, 855, 999
TscAI CASTG 4 cut(s) 64, 381, 779, 1228
TseFI GTSAC 2 cut(s) 352, 513
TseI GCWGC 1 cut(s) 984
Tsp45I GTSAC 2 cut(s) 352, 513
TspDTI ATGAA 7 cut(s) 87, 797, 1032, 1287, 1335, 1371, 1467
TspGWI ACGGA 1 cut(s) 1271
TspRI CASTG 4 cut(s) 64, 381, 779, 1228
Tth111I GACNNNGTC 1 cut(s) 1118
XagI CCTNNNNNAGG 1 cut(s) 971
XapI RAATTY 4 cut(s) 75, 490, 859, 877
XbaI TCTAGA 1 cut(s) 1438
XceI RCATGY 1 cut(s) 1097
XcmI CCANNNNNNNNNTGG 2 cut(s) 896, 1369
XmiI GTMKAC 1 cut(s) 769
XmnI GAANNNNTTC 2 cut(s) 396, 1153
XspI CTAG 3 cut(s) 30, 960, 1439
ZrmI AGTACT 1 cut(s) 396
Zsp2I ATGCAT 1 cut(s) 620
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.