Rmu_ssc0000204.1_g000050

F-box FBD LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000204.1
OG0004540
Physical Location & Seq
Forward (+)
234067 .. 235443
1377 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1377 bp
atgtctgacattcccgatcttgttattgacaaaatccttggactcattcctactaaagctgcgttccaggtgagctttctttgcaagcaacggcaagatgtagtctcctcattccctgtactcaatttcaacgaggatgatgattttgaccgccacaaccttaatcgcgatcgttaccgtcactacaaacatcaacacaaaaggttcatcaatttcttgaagggctatttagattatcgtaaaaagaaggaccagaaagagctcctgaataaattaagtcttcacatgaggatgttttcgcgtcgagattcatctaccataaccgagtggttgaaatatgcatgtgagagaggcctcaaagagttggatatcagtcctaaaatgagtgttgacccaacgagttggattgtcagtgagcgcccgccggaaagtctctacctcttctacatcttgccttggttggcgattgctagtgcaaaatcatctttaactagtctaaagttggagtgtgtgagagttccgcatctgctgcatgtaagagttccgcacgcttatgagcgactttttccatccttgaaaactctgtccttcaaaacttcacgggttgttgatctcgattctcttctatttgagtgcccgtccatggagtacctgtcattaactcaatgttcgattggaaactccgagttttatgttcaatgctcaagtcttaagtccttggaaattaagtattgccgtcttcatgttattcgagttgatcaagccttcaatcttgaatcttttacatttctctcaccgccgacatattcaagatgtgaaaagatgatcttgaagaatgccttcaacttgaaacatatcaacatttgtgttgattacctccgggaattttctttattgggaagccatcgtgcactagaggccacaattgaccgatatgtccatttgttcgagttctttgatggttatttgaatgccaacgtttctgttaaagcctatgaggccacaattttagttggggaacttttggaccaagaatggttctcgtcatattcgacacattttccgaagctgatgacctttcttagaaaatttagatgctgctcgaaaataaacctctacgttcaggctctcatcattccaaagaattatagaaagatgacctccgttccaccattgccggaaactttcactttgcaggcatggatgtcgaatccaccactagtgggaagtaaagtctatttggaaatggaggactccttgcaatggatggcaccactggcacagatattgatcatgccggtggacgttgaagaagatagaaagaaagtatacgagcgagctagtcctgtaggacatggacgaatgagaatggtatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

458

Amino Acids

53.62

Weight (kDa)

8.92

Isoelectric Point (pI)

44.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At5g56370 PF24758 156 - 286 1.5e-08 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 935
AccB1I GGYRCC 1 cut(s) 1270
AccI GTMKAC 1 cut(s) 1329
AccII CGCG 2 cut(s) 168, 301
AciI CCGC 5 cut(s) 151, 422, 521, 545, 797
AclI AACGTT 1 cut(s) 978
AcsI RAATTY 2 cut(s) 884, 1088
AfaI GTAC 2 cut(s) 120, 650
AfiI CCNNNNNNNGG 3 cut(s) 643, 1223, 1263
AflII CTTAAG 1 cut(s) 710
AhlI ACTAGT 2 cut(s) 491, 1219
AjnI CCWGG 1 cut(s) 66
AluBI AGCT 5 cut(s) 59, 75, 262, 1069, 1340
AluI AGCT 5 cut(s) 59, 75, 262, 1069, 1340
Alw21I GWGCWC 2 cut(s) 264, 913
Alw26I GTCTC 2 cut(s) 109, 437
Alw44I GTGCAC 1 cut(s) 909
AoxI GGCC 3 cut(s) 352, 918, 999
ApaLI GTGCAC 1 cut(s) 909
ApeKI GCWGC 3 cut(s) 59, 529, 1098
ApoI RAATTY 2 cut(s) 884, 1088
Asp700I GAANNNNTTC 1 cut(s) 839
AspLEI GCGC 1 cut(s) 420
AspS9I GGNCC 2 cut(s) 250, 1027
AsuC2I CCSGG 1 cut(s) 881
AsuHPI GGTGA 2 cut(s) 82, 786
AvaII GGWCC 2 cut(s) 250, 1027
BaeGI GKGCMC 2 cut(s) 638, 913
BanI GGYRCC 1 cut(s) 1270
BanII GRGCYC 1 cut(s) 264
BbsI GAAGAC 2 cut(s) 272, 731
Bbv12I GWGCWC 2 cut(s) 264, 913
BbvI GCAGC 3 cut(s) 46, 516, 1085
BccI CCATC 4 cut(s) 577, 912, 953, 1261
BceAI ACGGC 2 cut(s) 107, 720
BcgI CGANNNNNNTGC 2 cut(s) 1188, 1222
BciT130I CCWGG 1 cut(s) 68
BclI TGATCA 2 cut(s) 757, 1290
BcnI CCSGG 1 cut(s) 881
BcoDI GTCTC 2 cut(s) 109, 437
BcuI ACTAGT 2 cut(s) 491, 1219
BfaI CTAG 5 cut(s) 471, 492, 914, 1220, 1341
BfmI CTRYAG 1 cut(s) 1347
BfoI RGCGCY 1 cut(s) 421
BfrI CTTAAG 1 cut(s) 710
BglI GCCNNNNNGGC 1 cut(s) 998
BisI GCNGC 3 cut(s) 60, 530, 1099
BlsI GCNGC 3 cut(s) 61, 531, 1100
Bme1390I CCNGG 2 cut(s) 68, 881
Bme18I GGWCC 2 cut(s) 250, 1027
BmgT120I GGNCC 2 cut(s) 250, 1027
BmiI GGNNCC 1 cut(s) 1272
BmrFI CCNGG 2 cut(s) 68, 881
BmsI GCATC 2 cut(s) 532, 1085
BpiI GAAGAC 2 cut(s) 272, 731
BpuEI CTTGAG 1 cut(s) 688
BpuMI CCSGG 1 cut(s) 881
BsaBI GATNNNNATC 1 cut(s) 1289
BsaJI CCNNGG 4 cut(s) 37, 455, 642, 717
BsaXI ACNNNNNCTCC 2 cut(s) 638, 668
Bsc4I CCNNNNNNNGG 3 cut(s) 643, 1223, 1263
Bse118I RCCGGY 1 cut(s) 1297
Bse1I ACTGG 1 cut(s) 1281
Bse3DI GCAATG 2 cut(s) 1172, 1268
Bse8I GATNNNNATC 1 cut(s) 1289
BseBI CCWGG 1 cut(s) 68
BseDI CCNNGG 4 cut(s) 37, 455, 642, 717
BseGI GGATG 5 cut(s) 142, 297, 569, 1209, 1272
BseJI GATNNNNATC 1 cut(s) 1289
BseLI CCNNNNNNNGG 3 cut(s) 643, 1223, 1263
BseMI GCAATG 2 cut(s) 1172, 1268
BseNI ACTGG 1 cut(s) 1281
BseRI GAGGAG 1 cut(s) 97
BseSI GKGCMC 2 cut(s) 638, 913
BseXI GCAGC 3 cut(s) 46, 516, 1085
Bsh1236I CGCG 2 cut(s) 168, 301
Bsh1285I CGRYCG 1 cut(s) 172
BshFI GGCC 3 cut(s) 354, 920, 1001
BshNI GGYRCC 1 cut(s) 1270
BsiEI CGRYCG 1 cut(s) 172
BsiHKAI GWGCWC 2 cut(s) 264, 913
BsiSI CCGG 4 cut(s) 425, 880, 1178, 1298
BslI CCNNNNNNNGG 3 cut(s) 643, 1223, 1263
BsmAI GTCTC 2 cut(s) 109, 437
BsmI GAATGC 2 cut(s) 841, 976
BsnI GGCC 3 cut(s) 354, 920, 1001
Bsp1286I GDGCHC 3 cut(s) 264, 638, 913
Bsp143I GATC 6 cut(s) 16, 169, 610, 757, 825, 1290
Bsp19I CCATGG 1 cut(s) 642
Bsp68I TCGCGA 1 cut(s) 168
BspACI CCGC 5 cut(s) 151, 422, 521, 545, 797
BspANI GGCC 3 cut(s) 354, 920, 1001
BspFNI CGCG 2 cut(s) 168, 301
BspLI GGNNCC 1 cut(s) 1272
BspT107I GGYRCC 1 cut(s) 1270
BspTI CTTAAG 1 cut(s) 710
BsrDI GCAATG 2 cut(s) 1172, 1268
BsrFI RCCGGY 1 cut(s) 1297
BsrI ACTGG 1 cut(s) 1281
BssAI RCCGGY 1 cut(s) 1297
BssECI CCNNGG 4 cut(s) 37, 455, 642, 717
BssMI GATC 6 cut(s) 16, 169, 610, 757, 825, 1290
BssNAI GTATAC 1 cut(s) 1330
BssT1I CCWWGG 4 cut(s) 37, 455, 642, 717
Bst1107I GTATAC 1 cut(s) 1330
Bst2UI CCWGG 1 cut(s) 68
Bst4CI ACNGT 1 cut(s) 179
Bst6I CTCTTC 2 cut(s) 446, 627
BstAFI CTTAAG 1 cut(s) 710
BstAPI GCANNNNNTGC 1 cut(s) 529
BstC8I GCNNGC 5 cut(s) 86, 422, 549, 1197, 1338
BstDEI CTNAG 1 cut(s) 1082
BstDSI CCRYGG 1 cut(s) 642
BstF5I GGATG 5 cut(s) 142, 297, 569, 1209, 1272
BstFNI CGCG 2 cut(s) 168, 301
BstH2I RGCGCY 1 cut(s) 421
BstHHI GCGC 1 cut(s) 420
BstKTI GATC 6 cut(s) 19, 172, 613, 760, 828, 1293
BstMAI GTCTC 2 cut(s) 109, 437
BstMBI GATC 6 cut(s) 16, 169, 610, 757, 825, 1290
BstMCI CGRYCG 1 cut(s) 172
BstMWI GCNNNNNNNGC 5 cut(s) 81, 529, 917, 998, 1277
BstNI CCWGG 1 cut(s) 68
BstNSI RCATGY 2 cut(s) 345, 536
BstSCI CCNGG 2 cut(s) 66, 879
BstSFI CTRYAG 1 cut(s) 1347
BstSLI GKGCMC 2 cut(s) 638, 913
BstUI CGCG 2 cut(s) 168, 301
BstV1I GCAGC 3 cut(s) 46, 516, 1085
BstV2I GAAGAC 2 cut(s) 272, 731
BstXI CCANNNNNNTGG 1 cut(s) 402
BstZ17I GTATAC 1 cut(s) 1330
BsuRI GGCC 3 cut(s) 354, 920, 1001
BtgI CCRYGG 1 cut(s) 642
BtsCI GGATG 5 cut(s) 142, 297, 569, 1209, 1272
BtsIMutI CAGTG 2 cut(s) 418, 1274
BtuMI TCGCGA 1 cut(s) 168
Cac8I GCNNGC 5 cut(s) 86, 422, 549, 1197, 1338
CfoI GCGC 1 cut(s) 420
Cfr10I RCCGGY 1 cut(s) 1297
Cfr13I GGNCC 2 cut(s) 250, 1027
CseI GACGC 1 cut(s) 290
Csp6I GTAC 2 cut(s) 119, 649
CviAII CATG 8 cut(s) 286, 342, 533, 643, 743, 1200, 1294, 1355
CviQI GTAC 2 cut(s) 119, 649
DdeI CTNAG 1 cut(s) 1082
DpnI GATC 6 cut(s) 18, 171, 612, 759, 827, 1292
DpnII GATC 6 cut(s) 16, 169, 610, 757, 825, 1290
DrdI GACNNNNNNGTC 1 cut(s) 935
DseDI GACNNNNNNGTC 1 cut(s) 935
Eam1104I CTCTTC 2 cut(s) 446, 627
EarI CTCTTC 2 cut(s) 446, 627
Ecl136II GAGCTC 1 cut(s) 262
Eco130I CCWWGG 4 cut(s) 37, 455, 642, 717
Eco147I AGGCCT 1 cut(s) 354
Eco24I GRGCYC 1 cut(s) 264
Eco32I GATATC 1 cut(s) 370
Eco47I GGWCC 2 cut(s) 250, 1027
Eco53kI GAGCTC 1 cut(s) 262
EcoICRI GAGCTC 1 cut(s) 262
EcoRII CCWGG 1 cut(s) 66
EcoRV GATATC 1 cut(s) 370
EcoT14I CCWWGG 4 cut(s) 37, 455, 642, 717
EcoT22I ATGCAT 1 cut(s) 343
EcoT38I GRGCYC 1 cut(s) 264
ErhI CCWWGG 4 cut(s) 37, 455, 642, 717
FaeI CATG 8 cut(s) 289, 345, 536, 646, 746, 1203, 1297, 1358
FalI AAGNNNNNCTT 4 cut(s) 812, 844, 824, 856
FatI CATG 8 cut(s) 285, 341, 532, 642, 742, 1199, 1293, 1354
FauI CCCGC 1 cut(s) 429
FbaI TGATCA 2 cut(s) 757, 1290
FblI GTMKAC 1 cut(s) 1329
Fnu4HI GCNGC 3 cut(s) 60, 530, 1099
FokI GGATG 5 cut(s) 149, 304, 556, 1216, 1279
FriOI GRGCYC 1 cut(s) 264
Fsp4HI GCNGC 3 cut(s) 60, 530, 1099
FspBI CTAG 5 cut(s) 471, 492, 914, 1220, 1341
GlaI GCGC 1 cut(s) 419
GluI GCNGC 3 cut(s) 60, 530, 1099
HaeII RGCGCY 1 cut(s) 421
HaeIII GGCC 3 cut(s) 354, 920, 1001
HapII CCGG 4 cut(s) 425, 880, 1178, 1298
HgaI GACGC 1 cut(s) 290
HhaI GCGC 1 cut(s) 420
Hin1II CATG 8 cut(s) 289, 345, 536, 646, 746, 1203, 1297, 1358
Hin6I GCGC 1 cut(s) 418
HinP1I GCGC 1 cut(s) 418
HincII GTYRAC 1 cut(s) 391
HindII GTYRAC 1 cut(s) 391
HinfI GANTC 6 cut(s) 42, 308, 617, 776, 1210, 1253
HpaII CCGG 4 cut(s) 425, 880, 1178, 1298
HphI GGTGA 2 cut(s) 82, 786
Hpy166II GTNNAC 4 cut(s) 391, 911, 1303, 1330
Hpy188I TCNGA 3 cut(s) 7, 685, 1065
Hpy188III TCNNGA 9 cut(s) 14, 167, 217, 265, 305, 614, 773, 810, 829
Hpy8I GTNNAC 4 cut(s) 391, 911, 1303, 1330
Hpy99I CGWCG 1 cut(s) 306
HpyAV CCTTC 5 cut(s) 214, 241, 598, 775, 850
HpyCH4III ACNGT 1 cut(s) 179
HpyCH4IV ACGT 3 cut(s) 978, 1119, 1305
HpyCH4V TGCA 7 cut(s) 84, 341, 476, 532, 911, 1195, 1261
HpyF10VI GCNNNNNNNGC 5 cut(s) 81, 529, 917, 998, 1277
HpyF3I CTNAG 1 cut(s) 1082
HpySE526I ACGT 3 cut(s) 978, 1119, 1305
Hsp92II CATG 8 cut(s) 289, 345, 536, 646, 746, 1203, 1297, 1358
HspAI GCGC 1 cut(s) 418
Ksp22I TGATCA 2 cut(s) 757, 1290
Kzo9I GATC 6 cut(s) 16, 169, 610, 757, 825, 1290
LmnI GCTCC 1 cut(s) 267
Lsp1109I GCAGC 3 cut(s) 46, 516, 1085
LweI GCATC 2 cut(s) 532, 1085
MaeI CTAG 5 cut(s) 471, 492, 914, 1220, 1341
MaeII ACGT 3 cut(s) 978, 1119, 1305
MaeIII GTNAC 2 cut(s) 173, 179
MalI GATC 6 cut(s) 18, 171, 612, 759, 827, 1292
MboI GATC 6 cut(s) 16, 169, 610, 757, 825, 1290
MboII GAAGA 7 cut(s) 272, 433, 614, 731, 844, 1322, 1325
MfeI CAATTG 1 cut(s) 924
MhlI GDGCHC 3 cut(s) 264, 638, 913
MluCI AATT 9 cut(s) 124, 211, 272, 723, 884, 924, 1005, 1088, 1144
MlyI GAGTC 2 cut(s) 36, 1247
MmeI TCCRAC 3 cut(s) 345, 383, 483
Mph1103I ATGCAT 1 cut(s) 343
MroXI GAANNNNTTC 1 cut(s) 839
MseI TTAA 7 cut(s) 162, 275, 488, 659, 711, 726, 987
MslI CAYNNNNRTG 3 cut(s) 290, 552, 1298
MspCI CTTAAG 1 cut(s) 710
MspI CCGG 4 cut(s) 425, 880, 1178, 1298
MspR9I CCNGG 2 cut(s) 68, 881
MunI CAATTG 1 cut(s) 924
Mva1269I GAATGC 2 cut(s) 841, 976
MvaI CCWGG 1 cut(s) 68
MvnI CGCG 2 cut(s) 168, 301
MwoI GCNNNNNNNGC 5 cut(s) 81, 529, 917, 998, 1277
NciI CCSGG 1 cut(s) 881
NcoI CCATGG 1 cut(s) 642
NdeII GATC 6 cut(s) 16, 169, 610, 757, 825, 1290
NlaIII CATG 8 cut(s) 289, 345, 536, 646, 746, 1203, 1297, 1358
NlaIV GGNNCC 1 cut(s) 1272
NmuCI GTSAC 1 cut(s) 179
NruI TCGCGA 1 cut(s) 168
NsiI ATGCAT 1 cut(s) 343
NspI RCATGY 2 cut(s) 345, 536
PceI AGGCCT 1 cut(s) 354
PcsI WCGNNNNNNNCGW 1 cut(s) 1049
PctI GAATGC 2 cut(s) 841, 976
PdmI GAANNNNTTC 1 cut(s) 839
PfeI GAWTC 4 cut(s) 308, 617, 776, 1210
PfoI TCCNGGA 1 cut(s) 879
PkrI GCNGC 3 cut(s) 61, 531, 1100
Ple19I CGATCG 1 cut(s) 172
PleI GAGTC 2 cut(s) 36, 1247
PpsI GAGTC 2 cut(s) 36, 1247
Psp124BI GAGCTC 1 cut(s) 264
Psp1406I AACGTT 1 cut(s) 978
Psp6I CCWGG 1 cut(s) 66
PspGI CCWGG 1 cut(s) 66
PspN4I GGNNCC 1 cut(s) 1272
PspPI GGNCC 2 cut(s) 250, 1027
PvuI CGATCG 1 cut(s) 172
RruI TCGCGA 1 cut(s) 168
RsaI GTAC 2 cut(s) 120, 650
RsaNI GTAC 2 cut(s) 119, 649
RseI CAYNNNNRTG 3 cut(s) 290, 552, 1298
SacI GAGCTC 1 cut(s) 264
SaqAI TTAA 7 cut(s) 162, 275, 488, 659, 711, 726, 987
SatI GCNGC 3 cut(s) 60, 530, 1099
Sau3AI GATC 6 cut(s) 16, 169, 610, 757, 825, 1290
Sau96I GGNCC 2 cut(s) 250, 1027
SchI GAGTC 2 cut(s) 36, 1247
ScrFI CCNGG 2 cut(s) 68, 881
SduI GDGCHC 3 cut(s) 264, 638, 913
SfaNI GCATC 2 cut(s) 532, 1085
SfcI CTRYAG 1 cut(s) 1347
SinI GGWCC 2 cut(s) 250, 1027
SmiMI CAYNNNNRTG 3 cut(s) 290, 552, 1298
SmlI CTYRAG 2 cut(s) 703, 710
SmoI CTYRAG 2 cut(s) 703, 710
SpeI ACTAGT 2 cut(s) 491, 1219
Sse9I AATT 9 cut(s) 124, 211, 272, 723, 884, 924, 1005, 1088, 1144
SseBI AGGCCT 1 cut(s) 354
SsiI CCGC 5 cut(s) 151, 422, 521, 545, 797
SspMI CTAG 5 cut(s) 471, 492, 914, 1220, 1341
SstI GAGCTC 1 cut(s) 264
StuI AGGCCT 1 cut(s) 354
StyD4I CCNGG 2 cut(s) 66, 879
StyI CCWWGG 4 cut(s) 37, 455, 642, 717
TaaI ACNGT 1 cut(s) 179
TaiI ACGT 3 cut(s) 981, 1122, 1308
TaqI TCGA 8 cut(s) 304, 615, 671, 751, 948, 1052, 1103, 1208
TaqII GACCGA 1 cut(s) 945
TasI AATT 9 cut(s) 124, 211, 272, 723, 884, 924, 1005, 1088, 1144
TatI WGTACW 1 cut(s) 118
TfiI GAWTC 4 cut(s) 308, 617, 776, 1210
Tru1I TTAA 7 cut(s) 162, 275, 488, 659, 711, 726, 987
Tru9I TTAA 7 cut(s) 162, 275, 488, 659, 711, 726, 987
TscAI CASTG 2 cut(s) 418, 1281
TseFI GTSAC 1 cut(s) 179
TseI GCWGC 3 cut(s) 59, 529, 1098
Tsp45I GTSAC 1 cut(s) 179
TspDTI ATGAA 3 cut(s) 196, 300, 731
TspGWI ACGGA 1 cut(s) 1153
TspRI CASTG 2 cut(s) 418, 1281
Vha464I CTTAAG 1 cut(s) 710
VneI GTGCAC 1 cut(s) 909
VpaK11BI GGWCC 2 cut(s) 250, 1027
XapI RAATTY 2 cut(s) 884, 1088
XceI RCATGY 2 cut(s) 345, 536
XmiI GTMKAC 1 cut(s) 1329
XmnI GAANNNNTTC 1 cut(s) 839
XspI CTAG 5 cut(s) 471, 492, 914, 1220, 1341
Zsp2I ATGCAT 1 cut(s) 343
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.