Rroxscaffold_176G00430970

INO80 complex subunit D-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000176
OG0001489
Physical Location & Seq
Reverse (-)
48613 .. 52851
4239 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_176G00430970.1

Sequence Viewer

Length: 519 bp
ATGCTACAGAGACCACTACTGGGCCTTGATGGAGGACCTCAAGATCCACTACAGGGACTACTACTGGAACTACGGCGTCAGCCCCTCCAGTTTAAACCGCTGCAAGACAATGCCGATGACGGAAATATCAACCGCCGATGCGCTTCCGTTGGCTGTAAATTGACCGCCATGGCCTTGACGAGCTTTTGTCATCTCCATATTCTCTCGGATTCCAAGCAGAGGCTCTATAAGGCTTGCGATTATGTTATCAAGAGTGCTCAGGCAGGACCTATTACATGCGGGAAGCCAATATTAAGATCCACTACTCCTTCTCTTTGTGCTGTCCACTTCCAAAAGGCTCAGAAGCATGTTACCAGGGCCTTGCGGAAGGCAGGTCTTAATGTTTCCTCATCAAGTAAGCTTGCTCCTAAGTTCCATGTAATACTGGCAGAATATGTACGCCAAATTCAATCCATGCGAAAAGCTGCACGAAAGGAAAGTAGAAGTAAAGTTGCTATTAAGGAAGAAGCTGTCGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3Hc3H

Protein Analysis

172

Amino Acids

19.02

Weight (kDa)

9.95

Isoelectric Point (pI)

61.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC3H_KANSL2 PF13891 47 - 110 1e-16 KANL2-like, probable zinc-finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 362
AccI GTMKAC 1 cut(s) 513
AciI CCGC 5 cut(s) 98, 133, 165, 279, 364
AclWI GGATC 2 cut(s) 38, 291
AcsI RAATTY 1 cut(s) 444
AcyI GRCGYC 1 cut(s) 76
AfaI GTAC 1 cut(s) 438
AfiI CCNNNNNNNGG 3 cut(s) 20, 53, 219
AgsI TTSAA 1 cut(s) 449
AjnI CCWGG 1 cut(s) 353
AluBI AGCT 4 cut(s) 183, 400, 464, 509
AluI AGCT 4 cut(s) 183, 400, 464, 509
Alw21I GWGCWC 1 cut(s) 259
Alw26I GTCTC 1 cut(s) 4
AlwI GGATC 2 cut(s) 38, 291
AoxI GGCC 3 cut(s) 22, 171, 357
ApeKI GCWGC 2 cut(s) 100, 464
ApoI RAATTY 1 cut(s) 444
AspLEI GCGC 1 cut(s) 143
AspS9I GGNCC 4 cut(s) 22, 35, 266, 357
AvaII GGWCC 2 cut(s) 35, 266
Bbv12I GWGCWC 1 cut(s) 259
BbvI GCAGC 2 cut(s) 87, 451
BccI CCATC 1 cut(s) 23
BceAI ACGGC 1 cut(s) 89
BciT130I CCWGG 1 cut(s) 355
BcoDI GTCTC 1 cut(s) 4
BfmI CTRYAG 2 cut(s) 5, 50
BfuAI ACCTGC 1 cut(s) 362
BisI GCNGC 2 cut(s) 101, 465
BlsI GCNGC 2 cut(s) 102, 466
Bme1390I CCNGG 1 cut(s) 355
Bme18I GGWCC 2 cut(s) 35, 266
BmgT120I GGNCC 4 cut(s) 22, 35, 266, 357
BmrFI CCNGG 1 cut(s) 355
BmrI ACTGGG 1 cut(s) 29
BmsI GCATC 1 cut(s) 128
BmuI ACTGGG 1 cut(s) 29
BpmI CTGGAG 1 cut(s) 71
Bpu10I CCTNAGC 1 cut(s) 258
BpuEI CTTGAG 1 cut(s) 24
BsaHI GRCGYC 1 cut(s) 76
BsaI GGTCTC 1 cut(s) 4
BsaJI CCNNGG 2 cut(s) 168, 354
Bsc4I CCNNNNNNNGG 3 cut(s) 20, 53, 219
Bse1I ACTGG 4 cut(s) 24, 69, 88, 429
BseBI CCWGG 1 cut(s) 355
BseDI CCNNGG 2 cut(s) 168, 354
BseLI CCNNNNNNNGG 3 cut(s) 20, 53, 219
BseMII CTCAG 2 cut(s) 272, 353
BseNI ACTGG 4 cut(s) 24, 69, 88, 429
BseXI GCAGC 2 cut(s) 87, 451
BsgI GTGCAG 1 cut(s) 450
BshFI GGCC 3 cut(s) 24, 173, 359
BsiHKAI GWGCWC 1 cut(s) 259
BslFI GGGAC 1 cut(s) 69
BslI CCNNNNNNNGG 3 cut(s) 20, 53, 219
BsmAI GTCTC 1 cut(s) 4
BsmFI GGGAC 1 cut(s) 69
BsnI GGCC 3 cut(s) 24, 173, 359
Bso31I GGTCTC 1 cut(s) 4
Bsp1286I GDGCHC 1 cut(s) 259
Bsp143I GATC 2 cut(s) 43, 296
Bsp19I CCATGG 1 cut(s) 168
BspACI CCGC 5 cut(s) 98, 133, 165, 279, 364
BspANI GGCC 3 cut(s) 24, 173, 359
BspCNI CTCAG 2 cut(s) 271, 352
BspMI ACCTGC 1 cut(s) 362
BspPI GGATC 2 cut(s) 38, 291
BspTNI GGTCTC 1 cut(s) 4
BsrI ACTGG 4 cut(s) 24, 69, 88, 429
BssECI CCNNGG 2 cut(s) 168, 354
BssMI GATC 2 cut(s) 43, 296
BssNI GRCGYC 1 cut(s) 76
BssT1I CCWWGG 1 cut(s) 168
Bst2UI CCWGG 1 cut(s) 355
BstACI GRCGYC 1 cut(s) 76
BstC8I GCNNGC 2 cut(s) 235, 402
BstDEI CTNAG 3 cut(s) 258, 339, 408
BstDSI CCRYGG 1 cut(s) 168
BstHHI GCGC 1 cut(s) 143
BstKTI GATC 2 cut(s) 46, 299
BstMAI GTCTC 1 cut(s) 4
BstMBI GATC 2 cut(s) 43, 296
BstNI CCWGG 1 cut(s) 355
BstNSI RCATGY 2 cut(s) 279, 350
BstSCI CCNGG 1 cut(s) 353
BstSFI CTRYAG 2 cut(s) 5, 50
BstV1I GCAGC 2 cut(s) 87, 451
BstX2I RGATCY 2 cut(s) 43, 296
BstYI RGATCY 2 cut(s) 43, 296
BsuRI GGCC 3 cut(s) 24, 173, 359
BtgI CCRYGG 1 cut(s) 168
BveI ACCTGC 1 cut(s) 362
Cac8I GCNNGC 2 cut(s) 235, 402
CfoI GCGC 1 cut(s) 143
Cfr13I GGNCC 4 cut(s) 22, 35, 266, 357
CseI GACGC 1 cut(s) 65
Csp6I GTAC 1 cut(s) 437
CviAII CATG 5 cut(s) 169, 276, 347, 416, 454
CviQI GTAC 1 cut(s) 437
DdeI CTNAG 3 cut(s) 258, 339, 408
DpnI GATC 2 cut(s) 45, 298
DpnII GATC 2 cut(s) 43, 296
DraI TTTAAA 1 cut(s) 94
Eco130I CCWWGG 1 cut(s) 168
Eco31I GGTCTC 1 cut(s) 4
Eco47I GGWCC 2 cut(s) 35, 266
EcoO109I RGGNCCY 3 cut(s) 35, 266, 357
EcoRII CCWGG 1 cut(s) 353
EcoT14I CCWWGG 1 cut(s) 168
ErhI CCWWGG 1 cut(s) 168
FaeI CATG 5 cut(s) 172, 279, 350, 419, 457
FaiI YATR 9 cut(s) 170, 198, 228, 243, 277, 348, 417, 435, 455
FaqI GGGAC 1 cut(s) 69
FatI CATG 5 cut(s) 168, 275, 346, 415, 453
FauI CCCGC 1 cut(s) 272
FblI GTMKAC 1 cut(s) 513
Fnu4HI GCNGC 2 cut(s) 101, 465
Fsp4HI GCNGC 2 cut(s) 101, 465
GlaI GCGC 1 cut(s) 142
GluI GCNGC 2 cut(s) 101, 465
GsuI CTGGAG 1 cut(s) 71
HaeIII GGCC 3 cut(s) 24, 173, 359
HgaI GACGC 1 cut(s) 65
HhaI GCGC 1 cut(s) 143
Hin1I GRCGYC 1 cut(s) 76
Hin1II CATG 5 cut(s) 172, 279, 350, 419, 457
Hin6I GCGC 1 cut(s) 141
HinP1I GCGC 1 cut(s) 141
HincII GTYRAC 1 cut(s) 514
HindII GTYRAC 1 cut(s) 514
HindIII AAGCTT 1 cut(s) 398
HinfI GANTC 1 cut(s) 209
Hpy166II GTNNAC 2 cut(s) 325, 514
Hpy188I TCNGA 2 cut(s) 208, 342
Hpy188III TCNNGA 2 cut(s) 41, 250
Hpy8I GTNNAC 2 cut(s) 325, 514
HpyAV CCTTC 2 cut(s) 318, 361
HpyCH4V TGCA 2 cut(s) 103, 467
HpyF3I CTNAG 3 cut(s) 258, 339, 408
Hsp92I GRCGYC 1 cut(s) 76
Hsp92II CATG 5 cut(s) 172, 279, 350, 419, 457
HspAI GCGC 1 cut(s) 141
Kzo9I GATC 2 cut(s) 43, 296
LmnI GCTCC 1 cut(s) 409
Lsp1109I GCAGC 2 cut(s) 87, 451
LweI GCATC 1 cut(s) 128
MaeIII GTNAC 1 cut(s) 349
MalI GATC 2 cut(s) 45, 298
MboI GATC 2 cut(s) 43, 296
MboII GAAGA 1 cut(s) 515
MflI RGATCY 2 cut(s) 43, 296
MhlI GDGCHC 1 cut(s) 259
MluCI AATT 2 cut(s) 158, 444
MnlI CCTC 5 cut(s) 26, 48, 95, 213, 397
MseI TTAA 4 cut(s) 93, 293, 378, 498
MspA1I CMGCKG 1 cut(s) 100
MspR9I CCNGG 1 cut(s) 355
MssI GTTTAAAC 1 cut(s) 94
MvaI CCWGG 1 cut(s) 355
NcoI CCATGG 1 cut(s) 168
NdeII GATC 2 cut(s) 43, 296
NlaIII CATG 5 cut(s) 172, 279, 350, 419, 457
NspI RCATGY 2 cut(s) 279, 350
PfeI GAWTC 1 cut(s) 209
PkrI GCNGC 2 cut(s) 102, 466
PmeI GTTTAAAC 1 cut(s) 94
PpuMI RGGWCCY 2 cut(s) 35, 266
Psp5II RGGWCCY 2 cut(s) 35, 266
Psp6I CCWGG 1 cut(s) 353
PspGI CCWGG 1 cut(s) 353
PspPI GGNCC 4 cut(s) 22, 35, 266, 357
PspPPI RGGWCCY 2 cut(s) 35, 266
PsuI RGATCY 2 cut(s) 43, 296
RsaI GTAC 1 cut(s) 438
RsaNI GTAC 1 cut(s) 437
SalI GTCGAC 1 cut(s) 512
SaqAI TTAA 4 cut(s) 93, 293, 378, 498
SatI GCNGC 2 cut(s) 101, 465
Sau3AI GATC 2 cut(s) 43, 296
Sau96I GGNCC 4 cut(s) 22, 35, 266, 357
ScrFI CCNGG 1 cut(s) 355
SduI GDGCHC 1 cut(s) 259
SetI ASST 7 cut(s) 40, 185, 271, 376, 402, 466, 511
SfaNI GCATC 1 cut(s) 128
SfcI CTRYAG 2 cut(s) 5, 50
SinI GGWCC 2 cut(s) 35, 266
SmlI CTYRAG 1 cut(s) 39
SmoI CTYRAG 1 cut(s) 39
Sse9I AATT 2 cut(s) 158, 444
SsiI CCGC 5 cut(s) 98, 133, 165, 279, 364
SspI AATATT 1 cut(s) 291
StyD4I CCNGG 1 cut(s) 353
StyI CCWWGG 1 cut(s) 168
TaqI TCGA 1 cut(s) 513
TasI AATT 2 cut(s) 158, 444
TfiI GAWTC 1 cut(s) 209
Tru1I TTAA 4 cut(s) 93, 293, 378, 498
Tru9I TTAA 4 cut(s) 93, 293, 378, 498
TseI GCWGC 2 cut(s) 100, 464
TspGWI ACGGA 2 cut(s) 135, 136
VpaK11BI GGWCC 2 cut(s) 35, 266
XapI RAATTY 1 cut(s) 444
XceI RCATGY 2 cut(s) 279, 350
XmiI GTMKAC 1 cut(s) 513
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.