Rroxscaffold_5G00352520

INO80 complex subunit D-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0001489
Physical Location & Seq
Forward (+)
29586546 .. 29589483
2938 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00352520.1

Sequence Viewer

Length: 789 bp
ATGGCTACCTCTGGTTCCAAGAACAACCACCCTCCTCCGTCTTCTTCCAAGCCTCCCAAGAACCAAATCGTCATCGCCGACCCACAAATCCCAAACCCCGGCCCCTCCACCTCCGCCATCACTCCTTCCCGTGAAGACGTGGTCCTCTCACGCGCCTCCCACCTCACTCGCCAAGAGCTCCTCCGCCGCCGCTCCCACAACCTCAAGGAGCTGACGAAATGCTACAAAGACCACTACTGGGGGTTCATGGAGCACCTCAAGATCCAGTACAGGGAGTACTACTGGAAGTACGGTGTCAGCCCTTTCAAAGAAGACAACAACGCCACCACCACCAATAACAACAACAACAACAACAATGGAGTCGCTGCTGTTGAAGGCAGTGATGACAACCACAATAACAATCGCCGCTGCGCTTCGGTTGGTTGTAAGTTGAAGGCCATGGCCTTAACCAGCTTCTGCCACCTCCACATTCTTTCGGATTCGAAGCAGAGGCTCTACAAGGCTTGCAATTATGTTATCAAGAGTGCTCAGGCAGGACCTATAACATGTGGGAAGCCAATACTGAGATCCACTACCCCTTCTCTTTGTACTGTCCACTTCCAAAAGGCTCAGAAGCATGTAACCAGGGCCTTGCGGAAGGCGGGGCTTAACGTTTCCTCATCGAGTAAACTTGCTCCCAAGTTCCATGTGATAGTGGCAGAATACGTACGCCAAATCCAATCCACACGAAGAGCCGCTCGAAAGGAAAATAAAAGTAAAGTTGCAATAAAGGAAGAAACTGCTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3Hc3H

Protein Analysis

262

Amino Acids

29.32

Weight (kDa)

9.9

Isoelectric Point (pI)

48.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC3H_KANSL2 PF13891 137 - 200 6.2e-17 KANL2-like, probable zinc-finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 192, 737
AccII CGCG 1 cut(s) 153
AciI CCGC 8 cut(s) 114, 184, 187, 190, 406, 634, 641, 735
AclI AACGTT 1 cut(s) 651
AclWI GGATC 2 cut(s) 256, 561
AfaI GTAC 5 cut(s) 269, 278, 290, 589, 708
AfiI CCNNNNNNNGG 3 cut(s) 98, 238, 271
AflIII ACRYGT 1 cut(s) 545
AgsI TTSAA 3 cut(s) 307, 374, 433
AjiI CACGTC 1 cut(s) 139
AjnI CCWGG 1 cut(s) 623
AluBI AGCT 3 cut(s) 178, 211, 453
AluI AGCT 3 cut(s) 178, 211, 453
Alw21I GWGCWC 3 cut(s) 180, 255, 529
AlwI GGATC 2 cut(s) 256, 561
AlwNI CAGNNNCTG 1 cut(s) 456
AoxI GGCC 4 cut(s) 100, 435, 441, 627
ApeKI GCWGC 2 cut(s) 365, 408
AspLEI GCGC 2 cut(s) 155, 413
AspS9I GGNCC 4 cut(s) 101, 142, 536, 627
AsuC2I CCSGG 1 cut(s) 99
AsuII TTCGAA 1 cut(s) 482
AvaII GGWCC 2 cut(s) 142, 536
BanII GRGCYC 1 cut(s) 180
BbsI GAAGAC 3 cut(s) 33, 141, 318
Bbv12I GWGCWC 3 cut(s) 180, 255, 529
BbvI GCAGC 2 cut(s) 352, 395
BccI CCATC 1 cut(s) 125
BciT130I CCWGG 1 cut(s) 625
BcnI CCSGG 1 cut(s) 99
BisI GCNGC 6 cut(s) 187, 190, 366, 406, 409, 735
BlsI GCNGC 6 cut(s) 188, 191, 367, 407, 410, 736
BmcAI AGTACT 1 cut(s) 278
Bme1390I CCNGG 2 cut(s) 99, 625
Bme18I GGWCC 2 cut(s) 142, 536
BmgBI CACGTC 1 cut(s) 139
BmgT120I GGNCC 4 cut(s) 101, 142, 536, 627
BmiI GGNNCC 2 cut(s) 16, 103
BmrFI CCNGG 2 cut(s) 99, 625
BmrI ACTGGG 1 cut(s) 247
BmuI ACTGGG 1 cut(s) 247
BpiI GAAGAC 3 cut(s) 33, 141, 318
Bpu10I CCTNAGC 1 cut(s) 528
Bpu14I TTCGAA 1 cut(s) 482
BpuEI CTTGAG 2 cut(s) 188, 242
BpuMI CCSGG 1 cut(s) 99
BsaAI YACGTR 1 cut(s) 706
BsaJI CCNNGG 3 cut(s) 97, 438, 624
Bsc4I CCNNNNNNNGG 3 cut(s) 98, 238, 271
Bse1I ACTGG 3 cut(s) 242, 265, 287
BseBI CCWGG 1 cut(s) 625
BseDI CCNNGG 3 cut(s) 97, 438, 624
BseLI CCNNNNNNNGG 3 cut(s) 98, 238, 271
BseMII CTCAG 3 cut(s) 542, 554, 623
BseNI ACTGG 3 cut(s) 242, 265, 287
BseRI GAGGAG 2 cut(s) 24, 170
BseXI GCAGC 2 cut(s) 352, 395
Bsh1236I CGCG 1 cut(s) 153
BshFI GGCC 4 cut(s) 102, 437, 443, 629
BsiHKAI GWGCWC 3 cut(s) 180, 255, 529
BsiSI CCGG 1 cut(s) 99
BsiWI CGTACG 1 cut(s) 706
BslI CCNNNNNNNGG 3 cut(s) 98, 238, 271
BsnI GGCC 4 cut(s) 102, 437, 443, 629
Bsp119I TTCGAA 1 cut(s) 482
Bsp1286I GDGCHC 3 cut(s) 180, 255, 529
Bsp143I GATC 2 cut(s) 261, 566
Bsp19I CCATGG 1 cut(s) 438
BspACI CCGC 8 cut(s) 114, 184, 187, 190, 406, 634, 641, 735
BspANI GGCC 4 cut(s) 102, 437, 443, 629
BspCNI CTCAG 3 cut(s) 541, 555, 622
BspFNI CGCG 1 cut(s) 153
BspLI GGNNCC 2 cut(s) 16, 103
BspPI GGATC 2 cut(s) 256, 561
BspQI GCTCTTC 1 cut(s) 724
BspT104I TTCGAA 1 cut(s) 482
BsrBI CCGCTC 2 cut(s) 192, 737
BsrI ACTGG 3 cut(s) 242, 265, 287
BssECI CCNNGG 3 cut(s) 97, 438, 624
BssMI GATC 2 cut(s) 261, 566
BssT1I CCWWGG 1 cut(s) 438
Bst2UI CCWGG 1 cut(s) 625
Bst4CI ACNGT 2 cut(s) 293, 592
Bst6I CTCTTC 1 cut(s) 724
BstBAI YACGTR 1 cut(s) 706
BstBI TTCGAA 1 cut(s) 482
BstC8I GCNNGC 1 cut(s) 505
BstDEI CTNAG 3 cut(s) 528, 563, 609
BstDSI CCRYGG 1 cut(s) 438
BstFNI CGCG 1 cut(s) 153
BstHHI GCGC 2 cut(s) 155, 413
BstKTI GATC 2 cut(s) 264, 569
BstMBI GATC 2 cut(s) 261, 566
BstNI CCWGG 1 cut(s) 625
BstNSI RCATGY 2 cut(s) 549, 620
BstSCI CCNGG 2 cut(s) 97, 623
BstSNI TACGTA 1 cut(s) 706
BstUI CGCG 1 cut(s) 153
BstV1I GCAGC 2 cut(s) 352, 395
BstV2I GAAGAC 3 cut(s) 33, 141, 318
BstX2I RGATCY 2 cut(s) 261, 566
BstYI RGATCY 2 cut(s) 261, 566
BsuRI GGCC 4 cut(s) 102, 437, 443, 629
BtgI CCRYGG 1 cut(s) 438
BtgZI GCGATG 1 cut(s) 58
BtrI CACGTC 1 cut(s) 139
BtsI GCAGTG 1 cut(s) 385
BtsIMutI CAGTG 1 cut(s) 385
Cac8I GCNNGC 1 cut(s) 505
CaiI CAGNNNCTG 1 cut(s) 456
CfoI GCGC 2 cut(s) 155, 413
Cfr13I GGNCC 4 cut(s) 101, 142, 536, 627
Csp6I GTAC 5 cut(s) 268, 277, 289, 588, 707
CviAII CATG 5 cut(s) 247, 439, 546, 617, 686
CviQI GTAC 5 cut(s) 268, 277, 289, 588, 707
DdeI CTNAG 3 cut(s) 528, 563, 609
DpnI GATC 2 cut(s) 263, 568
DpnII GATC 2 cut(s) 261, 566
Eam1104I CTCTTC 1 cut(s) 724
EarI CTCTTC 1 cut(s) 724
EciI GGCGGA 2 cut(s) 103, 173
Ecl136II GAGCTC 1 cut(s) 178
Eco105I TACGTA 1 cut(s) 706
Eco130I CCWWGG 1 cut(s) 438
Eco24I GRGCYC 1 cut(s) 180
Eco47I GGWCC 2 cut(s) 142, 536
Eco53kI GAGCTC 1 cut(s) 178
EcoICRI GAGCTC 1 cut(s) 178
EcoO109I RGGNCCY 2 cut(s) 536, 627
EcoRII CCWGG 1 cut(s) 623
EcoT14I CCWWGG 1 cut(s) 438
EcoT38I GRGCYC 1 cut(s) 180
ErhI CCWWGG 1 cut(s) 438
FaeI CATG 5 cut(s) 250, 442, 549, 620, 689
FaiI YATR 7 cut(s) 248, 440, 513, 542, 547, 618, 687
FatI CATG 5 cut(s) 246, 438, 545, 616, 685
FauI CCCGC 1 cut(s) 634
Fnu4HI GCNGC 6 cut(s) 187, 190, 366, 406, 409, 735
FriOI GRGCYC 1 cut(s) 180
Fsp4HI GCNGC 6 cut(s) 187, 190, 366, 406, 409, 735
GlaI GCGC 2 cut(s) 154, 412
GluI GCNGC 6 cut(s) 187, 190, 366, 406, 409, 735
HaeIII GGCC 4 cut(s) 102, 437, 443, 629
HapII CCGG 1 cut(s) 99
HhaI GCGC 2 cut(s) 155, 413
Hin1II CATG 5 cut(s) 250, 442, 549, 620, 689
Hin6I GCGC 2 cut(s) 153, 411
HinP1I GCGC 2 cut(s) 153, 411
HinfI GANTC 2 cut(s) 360, 479
HpaII CCGG 1 cut(s) 99
Hpy166II GTNNAC 2 cut(s) 595, 668
Hpy188I TCNGA 2 cut(s) 478, 612
Hpy188III TCNNGA 2 cut(s) 259, 520
Hpy8I GTNNAC 2 cut(s) 595, 668
HpyAV CCTTC 5 cut(s) 135, 368, 427, 588, 631
HpyCH4III ACNGT 2 cut(s) 293, 592
HpyCH4IV ACGT 3 cut(s) 138, 651, 705
HpyCH4V TGCA 2 cut(s) 507, 764
HpyF3I CTNAG 3 cut(s) 528, 563, 609
HpySE526I ACGT 3 cut(s) 138, 651, 705
Hsp92II CATG 5 cut(s) 250, 442, 549, 620, 689
HspAI GCGC 2 cut(s) 153, 411
Kzo9I GATC 2 cut(s) 261, 566
LguI GCTCTTC 1 cut(s) 724
LmnI GCTCC 5 cut(s) 183, 197, 208, 250, 679
Lsp1109I GCAGC 2 cut(s) 352, 395
MaeII ACGT 3 cut(s) 138, 651, 705
MaeIII GTNAC 1 cut(s) 619
MalI GATC 2 cut(s) 263, 568
MbiI CCGCTC 2 cut(s) 192, 737
MboI GATC 2 cut(s) 261, 566
MboII GAAGA 6 cut(s) 33, 36, 146, 323, 741, 785
MflI RGATCY 2 cut(s) 261, 566
MhlI GDGCHC 3 cut(s) 180, 255, 529
MluCI AATT 1 cut(s) 508
MlyI GAGTC 1 cut(s) 369
MseI TTAA 2 cut(s) 446, 648
MspA1I CMGCKG 1 cut(s) 408
MspI CCGG 1 cut(s) 99
MspR9I CCNGG 2 cut(s) 99, 625
MvaI CCWGG 1 cut(s) 625
MvnI CGCG 1 cut(s) 153
NciI CCSGG 1 cut(s) 99
NcoI CCATGG 1 cut(s) 438
NdeII GATC 2 cut(s) 261, 566
NlaIII CATG 5 cut(s) 250, 442, 549, 620, 689
NlaIV GGNNCC 2 cut(s) 16, 103
NspI RCATGY 2 cut(s) 549, 620
NspV TTCGAA 1 cut(s) 482
PciI ACATGT 1 cut(s) 545
PciSI GCTCTTC 1 cut(s) 724
PcsI WCGNNNNNNNCGW 1 cut(s) 75
PfeI GAWTC 1 cut(s) 479
Pfl23II CGTACG 1 cut(s) 706
PflFI GACNNNGTC 1 cut(s) 140
PkrI GCNGC 6 cut(s) 188, 191, 367, 407, 410, 736
PleI GAGTC 1 cut(s) 368
PpsI GAGTC 1 cut(s) 368
Ppu21I YACGTR 1 cut(s) 706
PpuMI RGGWCCY 1 cut(s) 536
PscI ACATGT 1 cut(s) 545
Psp124BI GAGCTC 1 cut(s) 180
Psp1406I AACGTT 1 cut(s) 651
Psp5II RGGWCCY 1 cut(s) 536
Psp6I CCWGG 1 cut(s) 623
PspGI CCWGG 1 cut(s) 623
PspLI CGTACG 1 cut(s) 706
PspN4I GGNNCC 2 cut(s) 16, 103
PspPI GGNCC 4 cut(s) 101, 142, 536, 627
PspPPI RGGWCCY 1 cut(s) 536
PstNI CAGNNNCTG 1 cut(s) 456
PsuI RGATCY 2 cut(s) 261, 566
PsyI GACNNNGTC 1 cut(s) 140
RsaI GTAC 5 cut(s) 269, 278, 290, 589, 708
RsaNI GTAC 5 cut(s) 268, 277, 289, 588, 707
SacI GAGCTC 1 cut(s) 180
SapI GCTCTTC 1 cut(s) 724
SaqAI TTAA 2 cut(s) 446, 648
SatI GCNGC 6 cut(s) 187, 190, 366, 406, 409, 735
Sau3AI GATC 2 cut(s) 261, 566
Sau96I GGNCC 4 cut(s) 101, 142, 536, 627
ScaI AGTACT 1 cut(s) 278
SchI GAGTC 1 cut(s) 369
ScrFI CCNGG 2 cut(s) 99, 625
SduI GDGCHC 3 cut(s) 180, 255, 529
SfuI TTCGAA 1 cut(s) 482
SinI GGWCC 2 cut(s) 142, 536
SmlI CTYRAG 2 cut(s) 203, 257
SmoI CTYRAG 2 cut(s) 203, 257
SnaBI TACGTA 1 cut(s) 706
Sse9I AATT 1 cut(s) 508
SsiI CCGC 8 cut(s) 114, 184, 187, 190, 406, 634, 641, 735
SstI GAGCTC 1 cut(s) 180
StyD4I CCNGG 2 cut(s) 97, 623
StyI CCWWGG 1 cut(s) 438
TaaI ACNGT 2 cut(s) 293, 592
TaiI ACGT 3 cut(s) 141, 654, 708
TaqI TCGA 3 cut(s) 482, 662, 739
TasI AATT 1 cut(s) 508
TatI WGTACW 3 cut(s) 267, 276, 587
TauI GCSGC 4 cut(s) 189, 192, 408, 737
TfiI GAWTC 1 cut(s) 479
Tru1I TTAA 2 cut(s) 446, 648
Tru9I TTAA 2 cut(s) 446, 648
TscAI CASTG 1 cut(s) 385
TseI GCWGC 2 cut(s) 365, 408
TspDTI ATGAA 1 cut(s) 235
TspGWI ACGGA 1 cut(s) 27
TspRI CASTG 1 cut(s) 385
Tth111I GACNNNGTC 1 cut(s) 140
VpaK11BI GGWCC 2 cut(s) 142, 536
XceI RCATGY 2 cut(s) 549, 620
ZrmI AGTACT 1 cut(s) 278
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.