Rroxscaffold_2G00149000

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0004114
Physical Location & Seq
Reverse (-)
86607134 .. 86608237
1104 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00149000.1

Sequence Viewer

Length: 876 bp
ATGACATTGTGTTTCTTATTCTGCTCGATATATAAGCTATTGGAACAAATTGATCATGTTCGTTTTGCTGCCGTTTGTAAGGAATGGCGTTCTGTGGAAAAGGATTATAACCAAACAATGCAGCGCTGGTGTAAGGCACCTCCCATGCTTATGATCCCTATCAAATCCCAAGAGAAAATAAAATATGGCCTCTATGGCGTTTCTGATAGAACACTCTACAGCAATATTCGGTTACCAGTGACTTATGGTGAGAAGTGTTATGGCTCTAGCCATGCATTCTTCAATGACCACGTGCCGTTGATTTTCATGAAAGGAGGACAAAAAGATTGGAATTACAGATACTTTAAGGGTAGTGTTTCTGATGCCAGGTTTTTTAAAGCTCTATTTTATGAAAAATTCGTTTACGCAGTTAGAGATGAAGCACAAGCAGACACTATTAAGGTGACCAGATTCGACATCCATAGCTGTCATGATGTATATGTAATGGATCATAGTGTTAGATCTAGTACTTCTTTGGAAACATATCATTGTGAGAAGAATTATCTTGTGGAATCAACTAAGGGAGACCTATTGCTTGTTCGAACAGTTTCTACATGGGAAGGTCTGGAAATAACTCTGCAAGCTACTACAACCTTTAGGGTTCACAAATTTGTGTTCCAAGACAATGATTTCGAGCTGATTGAGGAAACAAATATTGGAGATGACGCTCTATTCGTAGGTGATAGTCATTCGGTGTCTGTTTTAGCTTCAAACTTTCCTATGTGCAAGCCAAACTCCATATACTACACATGTGCAGTACCTAATGAGTATGGTGGTAAAGCATGTGAGATATGTATCTTCAATTTAGAGGACGGAACCATATATAACAAACCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box F-box-like

Protein Analysis

291

Amino Acids

33.7

Weight (kDa)

6.15

Isoelectric Point (pI)

30.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 98 - 281 5e-21 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 108
AccB1I GGYRCC 1 cut(s) 136
AclWI GGATC 2 cut(s) 148, 495
AcsI RAATTY 2 cut(s) 395, 647
AcvI CACGTG 1 cut(s) 292
AfaI GTAC 2 cut(s) 508, 798
AfeI AGCGCT 1 cut(s) 125
AflIII ACRYGT 1 cut(s) 788
AgsI TTSAA 3 cut(s) 283, 750, 841
AjnI CCWGG 1 cut(s) 365
AjuI GAANNNNNNNTTGG 2 cut(s) 678, 710
AluBI AGCT 6 cut(s) 37, 380, 465, 623, 676, 746
AluI AGCT 6 cut(s) 37, 380, 465, 623, 676, 746
Alw26I GTCTC 1 cut(s) 558
AlwI GGATC 2 cut(s) 148, 495
Aor51HI AGCGCT 1 cut(s) 125
AoxI GGCC 1 cut(s) 187
ApeKI GCWGC 2 cut(s) 68, 121
ApoI RAATTY 2 cut(s) 395, 647
ArsI GACNNNNNNTTYG 4 cut(s) 653, 685, 695, 727
Asp700I GAANNNNTTC 1 cut(s) 586
AspLEI GCGC 1 cut(s) 126
AsuHPI GGTGA 3 cut(s) 260, 454, 731
AsuII TTCGAA 1 cut(s) 580
BanI GGYRCC 1 cut(s) 136
BbrPI CACGTG 1 cut(s) 292
BbvI GCAGC 2 cut(s) 55, 133
BceAI ACGGC 2 cut(s) 56, 280
BciT130I CCWGG 1 cut(s) 367
BclI TGATCA 1 cut(s) 52
BcoDI GTCTC 1 cut(s) 558
BfaI CTAG 2 cut(s) 267, 504
BfmI CTRYAG 1 cut(s) 217
BfoI RGCGCY 1 cut(s) 127
BglI GCCNNNNNGGC 1 cut(s) 195
BglII AGATCT 1 cut(s) 500
BisI GCNGC 2 cut(s) 69, 122
BlsI GCNGC 2 cut(s) 70, 123
BmcAI AGTACT 1 cut(s) 508
Bme1390I CCNGG 1 cut(s) 367
BmiI GGNNCC 2 cut(s) 138, 856
BmrFI CCNGG 1 cut(s) 367
BmsI GCATC 1 cut(s) 352
Bpu14I TTCGAA 1 cut(s) 580
BsaAI YACGTR 1 cut(s) 292
BsaBI GATNNNNATC 2 cut(s) 158, 833
BsaI GGTCTC 1 cut(s) 558
Bse1I ACTGG 1 cut(s) 236
Bse8I GATNNNNATC 2 cut(s) 158, 833
BseBI CCWGG 1 cut(s) 367
BseGI GGATG 1 cut(s) 456
BseJI GATNNNNATC 2 cut(s) 158, 833
BseNI ACTGG 1 cut(s) 236
BseXI GCAGC 2 cut(s) 55, 133
BsgI GTGCAG 1 cut(s) 813
BshFI GGCC 1 cut(s) 189
BshNI GGYRCC 1 cut(s) 136
BsmAI GTCTC 1 cut(s) 558
BsmI GAATGC 1 cut(s) 275
BsnI GGCC 1 cut(s) 189
Bso31I GGTCTC 1 cut(s) 558
Bsp119I TTCGAA 1 cut(s) 580
Bsp143I GATC 4 cut(s) 52, 153, 487, 500
BspANI GGCC 1 cut(s) 189
BspHI TCATGA 2 cut(s) 306, 469
BspLI GGNNCC 2 cut(s) 138, 856
BspPI GGATC 2 cut(s) 148, 495
BspT104I TTCGAA 1 cut(s) 580
BspT107I GGYRCC 1 cut(s) 136
BspTNI GGTCTC 1 cut(s) 558
BsrI ACTGG 1 cut(s) 236
BssMI GATC 4 cut(s) 52, 153, 487, 500
Bst2UI CCWGG 1 cut(s) 367
Bst4CI ACNGT 1 cut(s) 586
BstBAI YACGTR 1 cut(s) 292
BstBI TTCGAA 1 cut(s) 580
BstC8I GCNNGC 2 cut(s) 621, 767
BstDEI CTNAG 1 cut(s) 558
BstEII GGTNACC 2 cut(s) 231, 442
BstF5I GGATG 1 cut(s) 456
BstH2I RGCGCY 1 cut(s) 127
BstHHI GCGC 1 cut(s) 126
BstKTI GATC 4 cut(s) 55, 156, 490, 503
BstMAI GTCTC 1 cut(s) 558
BstMBI GATC 4 cut(s) 52, 153, 487, 500
BstMWI GCNNNNNNNGC 1 cut(s) 195
BstNI CCWGG 1 cut(s) 367
BstNSI RCATGY 2 cut(s) 792, 825
BstPI GGTNACC 2 cut(s) 231, 442
BstSCI CCNGG 1 cut(s) 365
BstSFI CTRYAG 1 cut(s) 217
BstV1I GCAGC 2 cut(s) 55, 133
BstX2I RGATCY 1 cut(s) 500
BstYI RGATCY 1 cut(s) 500
BsuRI GGCC 1 cut(s) 189
BtsCI GGATG 1 cut(s) 456
BtsIMutI CAGTG 1 cut(s) 243
Cac8I GCNNGC 2 cut(s) 621, 767
CciI TCATGA 2 cut(s) 306, 469
CfoI GCGC 1 cut(s) 126
CseI GACGC 1 cut(s) 713
Csp6I GTAC 2 cut(s) 507, 797
CviAII CATG 8 cut(s) 56, 145, 272, 307, 470, 594, 789, 822
CviQI GTAC 2 cut(s) 507, 797
DdeI CTNAG 1 cut(s) 558
DpnI GATC 4 cut(s) 54, 155, 489, 502
DpnII GATC 4 cut(s) 52, 153, 487, 500
DraI TTTAAA 1 cut(s) 376
Eco31I GGTCTC 1 cut(s) 558
Eco47III AGCGCT 1 cut(s) 125
Eco72I CACGTG 1 cut(s) 292
Eco91I GGTNACC 2 cut(s) 231, 442
EcoO65I GGTNACC 2 cut(s) 231, 442
EcoRII CCWGG 1 cut(s) 365
EcoT22I ATGCAT 1 cut(s) 277
FaeI CATG 8 cut(s) 59, 148, 275, 310, 473, 597, 792, 825
FatI CATG 8 cut(s) 55, 144, 271, 306, 469, 593, 788, 821
FbaI TGATCA 1 cut(s) 52
Fnu4HI GCNGC 2 cut(s) 69, 122
FokI GGATG 1 cut(s) 443
Fsp4HI GCNGC 2 cut(s) 69, 122
FspBI CTAG 2 cut(s) 267, 504
GlaI GCGC 1 cut(s) 125
GluI GCNGC 2 cut(s) 69, 122
HaeII RGCGCY 1 cut(s) 127
HaeIII GGCC 1 cut(s) 189
HgaI GACGC 1 cut(s) 713
HhaI GCGC 1 cut(s) 126
Hin1II CATG 8 cut(s) 59, 148, 275, 310, 473, 597, 792, 825
Hin6I GCGC 1 cut(s) 124
HinP1I GCGC 1 cut(s) 124
HinfI GANTC 2 cut(s) 450, 551
HphI GGTGA 3 cut(s) 260, 454, 731
Hpy166II GTNNAC 2 cut(s) 403, 643
Hpy188I TCNGA 2 cut(s) 205, 361
Hpy188III TCNNGA 3 cut(s) 307, 470, 605
Hpy8I GTNNAC 2 cut(s) 403, 643
HpyAV CCTTC 1 cut(s) 593
HpyCH4III ACNGT 1 cut(s) 586
HpyCH4IV ACGT 1 cut(s) 291
HpyCH4V TGCA 5 cut(s) 121, 275, 619, 765, 794
HpyF10VI GCNNNNNNNGC 1 cut(s) 195
HpyF3I CTNAG 1 cut(s) 558
HpySE526I ACGT 1 cut(s) 291
Hsp92II CATG 8 cut(s) 59, 148, 275, 310, 473, 597, 792, 825
HspAI GCGC 1 cut(s) 124
Ksp22I TGATCA 1 cut(s) 52
Kzo9I GATC 4 cut(s) 52, 153, 487, 500
LpnPI CCDG 6 cut(s) 112, 249, 352, 379, 460, 590
Lsp1109I GCAGC 2 cut(s) 55, 133
LweI GCATC 1 cut(s) 352
MaeI CTAG 2 cut(s) 267, 504
MaeII ACGT 1 cut(s) 291
MaeIII GTNAC 3 cut(s) 231, 238, 442
MalI GATC 4 cut(s) 54, 155, 489, 502
MboI GATC 4 cut(s) 52, 153, 487, 500
MboII GAAGA 3 cut(s) 271, 547, 829
MflI RGATCY 1 cut(s) 500
MluCI AATT 6 cut(s) 48, 331, 395, 538, 647, 841
MnlI CCTC 5 cut(s) 150, 200, 308, 676, 841
Mph1103I ATGCAT 1 cut(s) 277
MroXI GAANNNNTTC 1 cut(s) 586
MseI TTAA 3 cut(s) 345, 375, 438
MslI CAYNNNNRTG 1 cut(s) 149
MspR9I CCNGG 1 cut(s) 367
Mva1269I GAATGC 1 cut(s) 275
MvaI CCWGG 1 cut(s) 367
MwoI GCNNNNNNNGC 1 cut(s) 195
NdeII GATC 4 cut(s) 52, 153, 487, 500
NlaIII CATG 8 cut(s) 59, 148, 275, 310, 473, 597, 792, 825
NlaIV GGNNCC 2 cut(s) 138, 856
NmuCI GTSAC 2 cut(s) 238, 442
NsiI ATGCAT 1 cut(s) 277
NspI RCATGY 2 cut(s) 792, 825
NspV TTCGAA 1 cut(s) 580
PagI TCATGA 2 cut(s) 306, 469
PciI ACATGT 1 cut(s) 788
PcsI WCGNNNNNNNCGW 1 cut(s) 711
PctI GAATGC 1 cut(s) 275
PdmI GAANNNNTTC 1 cut(s) 586
PfeI GAWTC 2 cut(s) 450, 551
PkrI GCNGC 2 cut(s) 70, 123
PmaCI CACGTG 1 cut(s) 292
PmlI CACGTG 1 cut(s) 292
Ppu21I YACGTR 1 cut(s) 292
PscI ACATGT 1 cut(s) 788
PsiI TTATAA 1 cut(s) 108
Psp6I CCWGG 1 cut(s) 365
PspCI CACGTG 1 cut(s) 292
PspEI GGTNACC 2 cut(s) 231, 442
PspGI CCWGG 1 cut(s) 365
PspN4I GGNNCC 2 cut(s) 138, 856
PsrI GAACNNNNNNTAC 2 cut(s) 574, 606
PsuI RGATCY 1 cut(s) 500
RsaI GTAC 2 cut(s) 508, 798
RsaNI GTAC 2 cut(s) 507, 797
RseI CAYNNNNRTG 1 cut(s) 149
SaqAI TTAA 3 cut(s) 345, 375, 438
SatI GCNGC 2 cut(s) 69, 122
Sau3AI GATC 4 cut(s) 52, 153, 487, 500
ScaI AGTACT 1 cut(s) 508
ScrFI CCNGG 1 cut(s) 367
SfaNI GCATC 1 cut(s) 352
SfcI CTRYAG 1 cut(s) 217
SfuI TTCGAA 1 cut(s) 580
SmiMI CAYNNNNRTG 1 cut(s) 149
Sse9I AATT 6 cut(s) 48, 331, 395, 538, 647, 841
SspI AATATT 2 cut(s) 226, 694
SspMI CTAG 2 cut(s) 267, 504
StyD4I CCNGG 1 cut(s) 365
TaaI ACNGT 1 cut(s) 586
TaiI ACGT 1 cut(s) 294
TaqI TCGA 4 cut(s) 26, 453, 580, 672
TasI AATT 6 cut(s) 48, 331, 395, 538, 647, 841
TatI WGTACW 1 cut(s) 506
TfiI GAWTC 2 cut(s) 450, 551
Tru1I TTAA 3 cut(s) 345, 375, 438
Tru9I TTAA 3 cut(s) 345, 375, 438
TscAI CASTG 1 cut(s) 243
TseFI GTSAC 2 cut(s) 238, 442
TseI GCWGC 2 cut(s) 68, 121
Tsp45I GTSAC 2 cut(s) 238, 442
TspDTI ATGAA 4 cut(s) 295, 323, 405, 432
TspGWI ACGGA 1 cut(s) 867
TspRI CASTG 1 cut(s) 243
XapI RAATTY 2 cut(s) 395, 647
XceI RCATGY 2 cut(s) 792, 825
XmnI GAANNNNTTC 1 cut(s) 586
XspI CTAG 2 cut(s) 267, 504
ZrmI AGTACT 1 cut(s) 508
Zsp2I ATGCAT 1 cut(s) 277
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.