Rroxscaffold_3G00222310

dnaJ homolog subfamily C member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0002514
Physical Location & Seq
Forward (+)
5343119 .. 5343574
456 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_3G00222310.1

Sequence Viewer

Length: 456 bp
ATGAACAAGACTATAGATGAAGATCATTATAAGGTTCTTGATTTGCCGTCCGGCGAGGAAGGCTCTAAGCTCACAGAGAAACAGATTGCAAAGGCTTACCACGCAAAGGCTTTGGTGTTGCACCCGGACAAGAGGCCTAAAAGTGATCTCCAAGCCAATACCAACTTCCAAAGGCTCAAAAAATCCTATGAGGTTCTCAAGAATGAAAGTGCCCGCAAAGTGTTCGACAAAATCCTTAGAGATCAAAAACGAAGGATGGGCGCCATTTGGAGCGAGATCCCAAGCGACGAGAAGATGGTTTCGATCTTGAAGCAAGAGAACGAGCTGCAAAGTTTGACGGGGATGCCAAAGTTAGACAAGAACAAGATGAGAGTGAAGTGGAAGTTAGATGAAGAGTGTGCAAGATATCGTACAATGCCTCGGAAGAGAGAGCACGGGGAGTTGGGAAGAAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ RRM_1

Protein Analysis

151

Amino Acids

17.79

Weight (kDa)

9.73

Isoelectric Point (pI)

59.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 8 - 76 1.1e-14 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 30
AccB1I GGYRCC 1 cut(s) 260
AciI CCGC 1 cut(s) 214
AclWI GGATC 1 cut(s) 271
AcyI GRCGYC 1 cut(s) 261
AfaI GTAC 1 cut(s) 412
AfiI CCNNNNNNNGG 1 cut(s) 106
AgsI TTSAA 1 cut(s) 310
AjuI GAANNNNNNNTTGG 2 cut(s) 149, 181
AluBI AGCT 2 cut(s) 70, 325
AluI AGCT 2 cut(s) 70, 325
Alw21I GWGCWC 1 cut(s) 435
AlwI GGATC 1 cut(s) 271
AoxI GGCC 1 cut(s) 134
ApeKI GCWGC 1 cut(s) 325
AspLEI GCGC 1 cut(s) 263
AsuC2I CCSGG 1 cut(s) 125
BaeGI GKGCMC 1 cut(s) 214
BanI GGYRCC 1 cut(s) 260
Bbv12I GWGCWC 1 cut(s) 435
BbvI GCAGC 1 cut(s) 312
BccI CCATC 2 cut(s) 250, 289
BceAI ACGGC 1 cut(s) 31
BcgI CGANNNNNNTGC 2 cut(s) 205, 239
BcnI CCSGG 1 cut(s) 125
BfmI CTRYAG 1 cut(s) 12
BfoI RGCGCY 1 cut(s) 264
BisI GCNGC 1 cut(s) 326
BlsI GCNGC 1 cut(s) 327
Bme1390I CCNGG 1 cut(s) 125
BmiI GGNNCC 1 cut(s) 262
BmrFI CCNGG 1 cut(s) 125
BmsI GCATC 1 cut(s) 333
BplI GAGNNNNNCTC 2 cut(s) 47, 79
BpuEI CTTGAG 1 cut(s) 182
BpuMI CCSGG 1 cut(s) 125
BsaBI GATNNNNATC 1 cut(s) 21
BsaHI GRCGYC 1 cut(s) 261
BsaJI CCNNGG 1 cut(s) 419
Bsc4I CCNNNNNNNGG 1 cut(s) 106
Bse8I GATNNNNATC 1 cut(s) 21
BseDI CCNNGG 1 cut(s) 419
BseGI GGATG 2 cut(s) 261, 348
BseJI GATNNNNATC 1 cut(s) 21
BseLI CCNNNNNNNGG 1 cut(s) 106
BseSI GKGCMC 1 cut(s) 214
BseXI GCAGC 1 cut(s) 312
BshFI GGCC 1 cut(s) 136
BshNI GGYRCC 1 cut(s) 260
BsiHKAI GWGCWC 1 cut(s) 435
BsiSI CCGG 2 cut(s) 51, 125
BslI CCNNNNNNNGG 1 cut(s) 106
BsnI GGCC 1 cut(s) 136
Bsp1286I GDGCHC 2 cut(s) 214, 435
Bsp143I GATC 5 cut(s) 22, 145, 241, 276, 303
BspACI CCGC 1 cut(s) 214
BspANI GGCC 1 cut(s) 136
BspLI GGNNCC 1 cut(s) 262
BspPI GGATC 1 cut(s) 271
BspT107I GGYRCC 1 cut(s) 260
BssECI CCNNGG 1 cut(s) 419
BssMI GATC 5 cut(s) 22, 145, 241, 276, 303
BssNI GRCGYC 1 cut(s) 261
Bst6I CTCTTC 2 cut(s) 387, 419
BstACI GRCGYC 1 cut(s) 261
BstC8I GCNNGC 1 cut(s) 214
BstDEI CTNAG 2 cut(s) 66, 236
BstF5I GGATG 2 cut(s) 261, 348
BstH2I RGCGCY 1 cut(s) 264
BstHHI GCGC 1 cut(s) 263
BstKTI GATC 5 cut(s) 25, 148, 244, 279, 306
BstMBI GATC 5 cut(s) 22, 145, 241, 276, 303
BstMWI GCNNNNNNNGC 2 cut(s) 60, 101
BstSCI CCNGG 1 cut(s) 123
BstSFI CTRYAG 1 cut(s) 12
BstSLI GKGCMC 1 cut(s) 214
BstV1I GCAGC 1 cut(s) 312
BstX2I RGATCY 1 cut(s) 276
BstYI RGATCY 1 cut(s) 276
BsuRI GGCC 1 cut(s) 136
BtsCI GGATG 2 cut(s) 261, 348
Cac8I GCNNGC 1 cut(s) 214
CfoI GCGC 1 cut(s) 263
Csp6I GTAC 1 cut(s) 411
CviJI RGCY 8 cut(s) 63, 70, 95, 110, 136, 155, 175, 325
CviKI_1 RGCY 8 cut(s) 63, 70, 95, 110, 136, 155, 175, 325
CviQI GTAC 1 cut(s) 411
DdeI CTNAG 2 cut(s) 66, 236
DinI GGCGCC 1 cut(s) 262
DpnI GATC 5 cut(s) 24, 147, 243, 278, 305
DpnII GATC 5 cut(s) 22, 145, 241, 276, 303
Eam1104I CTCTTC 2 cut(s) 387, 419
EarI CTCTTC 2 cut(s) 387, 419
Eco147I AGGCCT 1 cut(s) 136
Eco32I GATATC 1 cut(s) 407
EcoRV GATATC 1 cut(s) 407
EgeI GGCGCC 1 cut(s) 262
EheI GGCGCC 1 cut(s) 262
FaiI YATR 3 cut(s) 14, 30, 189
FauI CCCGC 1 cut(s) 221
Fnu4HI GCNGC 1 cut(s) 326
FokI GGATG 2 cut(s) 268, 355
Fsp4HI GCNGC 1 cut(s) 326
GlaI GCGC 1 cut(s) 262
GluI GCNGC 1 cut(s) 326
HaeII RGCGCY 1 cut(s) 264
HaeIII GGCC 1 cut(s) 136
HapII CCGG 2 cut(s) 51, 125
HhaI GCGC 1 cut(s) 263
Hin1I GRCGYC 1 cut(s) 261
Hin6I GCGC 1 cut(s) 261
HinP1I GCGC 1 cut(s) 261
HpaII CCGG 2 cut(s) 51, 125
Hpy188I TCNGA 1 cut(s) 423
Hpy188III TCNNGA 3 cut(s) 38, 199, 307
Hpy99I CGWCG 1 cut(s) 290
HpyAV CCTTC 3 cut(s) 53, 246, 444
HpyCH4V TGCA 4 cut(s) 89, 121, 328, 401
HpyF10VI GCNNNNNNNGC 2 cut(s) 60, 101
HpyF3I CTNAG 2 cut(s) 66, 236
Hsp92I GRCGYC 1 cut(s) 261
HspAI GCGC 1 cut(s) 261
KasI GGCGCC 1 cut(s) 260
Kzo9I GATC 5 cut(s) 22, 145, 241, 276, 303
LmnI GCTCC 1 cut(s) 270
LpnPI CCDG 2 cut(s) 64, 138
Lsp1109I GCAGC 1 cut(s) 312
LweI GCATC 1 cut(s) 333
MalI GATC 5 cut(s) 24, 147, 243, 278, 305
MboI GATC 5 cut(s) 22, 145, 241, 276, 303
MboII GAAGA 4 cut(s) 32, 304, 404, 436
MflI RGATCY 1 cut(s) 276
MhlI GDGCHC 2 cut(s) 214, 435
Mly113I GGCGCC 1 cut(s) 261
MnlI CCTC 4 cut(s) 49, 126, 184, 429
MspI CCGG 2 cut(s) 51, 125
MspR9I CCNGG 1 cut(s) 125
MwoI GCNNNNNNNGC 2 cut(s) 60, 101
NarI GGCGCC 1 cut(s) 261
NciI CCSGG 1 cut(s) 125
NdeII GATC 5 cut(s) 22, 145, 241, 276, 303
NlaIV GGNNCC 1 cut(s) 262
PceI AGGCCT 1 cut(s) 136
PkrI GCNGC 1 cut(s) 327
PluTI GGCGCC 1 cut(s) 264
PsiI TTATAA 1 cut(s) 30
PspN4I GGNNCC 1 cut(s) 262
PsuI RGATCY 1 cut(s) 276
RsaI GTAC 1 cut(s) 412
RsaNI GTAC 1 cut(s) 411
SatI GCNGC 1 cut(s) 326
Sau3AI GATC 5 cut(s) 22, 145, 241, 276, 303
ScrFI CCNGG 1 cut(s) 125
SduI GDGCHC 2 cut(s) 214, 435
SetI ASST 5 cut(s) 36, 72, 195, 327, 455
SfaNI GCATC 1 cut(s) 333
SfcI CTRYAG 1 cut(s) 12
SfoI GGCGCC 1 cut(s) 262
SmlI CTYRAG 1 cut(s) 197
SmoI CTYRAG 1 cut(s) 197
SseBI AGGCCT 1 cut(s) 136
SsiI CCGC 1 cut(s) 214
SspDI GGCGCC 1 cut(s) 260
StuI AGGCCT 1 cut(s) 136
StyD4I CCNGG 1 cut(s) 123
TaqI TCGA 2 cut(s) 225, 302
TseI GCWGC 1 cut(s) 325
TspDTI ATGAA 4 cut(s) 17, 33, 219, 405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.