Rroxscaffold_3G00250450

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0002271
Physical Location & Seq
Forward (+)
44230248 .. 44231881
1634 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00250450.1

Sequence Viewer

Length: 426 bp
ATGCGAGTTAGTGTGGTCAAGATGAGTGGACACTCCTCATCAGCGGCAGCTCTGTTAGCAATGATGGTGGTGGTGATGACTATGTCCATCATCAAAACTGATTGCTTCAGCATCAACCAGACGATCACCGTCTCCTCTACTTGGTGTGATGGCCTCAACGACGACTTGTCGTGCCTAACTAAACAGACTGATTTGGATCCGGACGAGTTCATGCTGGACTCCGAATGCAGCAGAAGAATCTTGCCTTGTCAAGAATTCTACTCTGGTAAGACCTTAAATCCTGCAACGGCTGTGTCGTGTGACCGAAAGGGGTTTCCTCCGTGCCTCACTGACAGGAAAAACAAGAACCCCCAGCAGCACGCTGCAACGAGGCGCCTACCAATAGAGATTGCTACCGCTATAATTAGTATCTCTGCTTCTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

141

Amino Acids

15.35

Weight (kDa)

6.69

Isoelectric Point (pI)

62.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 372
AccIII TCCGGA 1 cut(s) 199
AciI CCGC 2 cut(s) 44, 396
AclWI GGATC 2 cut(s) 191, 204
AcsI RAATTY 1 cut(s) 254
AcuI CTGAAG 1 cut(s) 91
AcyI GRCGYC 1 cut(s) 373
AfiI CCNNNNNNNGG 1 cut(s) 141
AhdI GACNNNNNGTC 1 cut(s) 166
AluBI AGCT 1 cut(s) 50
AluI AGCT 1 cut(s) 50
Alw26I GTCTC 1 cut(s) 136
AlwI GGATC 2 cut(s) 191, 204
Aor13HI TCCGGA 1 cut(s) 199
AoxI GGCC 1 cut(s) 151
ApeKI GCWGC 4 cut(s) 47, 228, 355, 362
ApoI RAATTY 1 cut(s) 254
AspLEI GCGC 1 cut(s) 375
AsuHPI GGTGA 2 cut(s) 85, 118
BamHI GGATCC 1 cut(s) 196
BanI GGYRCC 1 cut(s) 372
BarI GAAGNNNNNNTAC 1 cut(s) 400
BbvI GCAGC 4 cut(s) 59, 240, 349, 367
BccI CCATC 3 cut(s) 58, 95, 143
BceAI ACGGC 1 cut(s) 303
BcoDI GTCTC 1 cut(s) 136
BfoI RGCGCY 1 cut(s) 376
BisI GCNGC 5 cut(s) 45, 48, 229, 356, 363
BlsI GCNGC 5 cut(s) 46, 49, 230, 357, 364
BmeRI GACNNNNNGTC 1 cut(s) 166
BmiI GGNNCC 2 cut(s) 198, 374
BmsI GCATC 1 cut(s) 120
BsaBI GATNNNNATC 1 cut(s) 195
BsaHI GRCGYC 1 cut(s) 373
BsaWI WCCGGW 1 cut(s) 199
Bsc4I CCNNNNNNNGG 1 cut(s) 141
Bse3DI GCAATG 1 cut(s) 66
Bse8I GATNNNNATC 1 cut(s) 195
BseAI TCCGGA 1 cut(s) 199
BseJI GATNNNNATC 1 cut(s) 195
BseLI CCNNNNNNNGG 1 cut(s) 141
BseMI GCAATG 1 cut(s) 66
BseRI GAGGAG 2 cut(s) 25, 124
BseXI GCAGC 4 cut(s) 59, 240, 349, 367
BseYI CCCAGC 1 cut(s) 351
BshFI GGCC 1 cut(s) 153
BshNI GGYRCC 1 cut(s) 372
BsiSI CCGG 1 cut(s) 200
BslI CCNNNNNNNGG 1 cut(s) 141
BsmAI GTCTC 1 cut(s) 136
BsmBI CGTCTC 1 cut(s) 136
BsmI GAATGC 1 cut(s) 230
BsnI GGCC 1 cut(s) 153
Bsp13I TCCGGA 1 cut(s) 199
Bsp143I GATC 2 cut(s) 123, 196
BspACI CCGC 2 cut(s) 44, 396
BspANI GGCC 1 cut(s) 153
BspEI TCCGGA 1 cut(s) 199
BspLI GGNNCC 2 cut(s) 198, 374
BspPI GGATC 2 cut(s) 191, 204
BspT107I GGYRCC 1 cut(s) 372
BsrDI GCAATG 1 cut(s) 66
BssMI GATC 2 cut(s) 123, 196
BssNI GRCGYC 1 cut(s) 373
Bst4CI ACNGT 1 cut(s) 130
BstACI GRCGYC 1 cut(s) 373
BstC8I GCNNGC 1 cut(s) 360
BstH2I RGCGCY 1 cut(s) 376
BstHHI GCGC 1 cut(s) 375
BstKTI GATC 2 cut(s) 126, 199
BstMAI GTCTC 1 cut(s) 136
BstMBI GATC 2 cut(s) 123, 196
BstMWI GCNNNNNNNGC 1 cut(s) 56
BstV1I GCAGC 4 cut(s) 59, 240, 349, 367
BstX2I RGATCY 1 cut(s) 196
BstYI RGATCY 1 cut(s) 196
BsuRI GGCC 1 cut(s) 153
BtsIMutI CAGTG 1 cut(s) 327
Cac8I GCNNGC 1 cut(s) 360
CfoI GCGC 1 cut(s) 375
CspCI CAANNNNNGTGG 4 cut(s) 7, 42, 48, 83
CviAII CATG 1 cut(s) 211
CviJI RGCY 3 cut(s) 50, 153, 290
CviKI_1 RGCY 3 cut(s) 50, 153, 290
DinI GGCGCC 1 cut(s) 374
DpnI GATC 2 cut(s) 125, 198
DpnII GATC 2 cut(s) 123, 196
DriI GACNNNNNGTC 1 cut(s) 166
Eam1105I GACNNNNNGTC 1 cut(s) 166
Eco57I CTGAAG 1 cut(s) 91
EcoRI GAATTC 1 cut(s) 254
EgeI GGCGCC 1 cut(s) 374
EheI GGCGCC 1 cut(s) 374
Esp3I CGTCTC 1 cut(s) 136
FaeI CATG 1 cut(s) 214
FaiI YATR 3 cut(s) 83, 212, 401
FatI CATG 1 cut(s) 210
Fnu4HI GCNGC 5 cut(s) 45, 48, 229, 356, 363
Fsp4HI GCNGC 5 cut(s) 45, 48, 229, 356, 363
GlaI GCGC 1 cut(s) 374
GluI GCNGC 5 cut(s) 45, 48, 229, 356, 363
GsaI CCCAGC 1 cut(s) 355
HaeII RGCGCY 1 cut(s) 376
HaeIII GGCC 1 cut(s) 153
HapII CCGG 1 cut(s) 200
HhaI GCGC 1 cut(s) 375
Hin1I GRCGYC 1 cut(s) 373
Hin1II CATG 1 cut(s) 214
Hin6I GCGC 1 cut(s) 373
HinP1I GCGC 1 cut(s) 373
HinfI GANTC 2 cut(s) 218, 237
HpaII CCGG 1 cut(s) 200
HphI GGTGA 2 cut(s) 85, 118
Hpy166II GTNNAC 1 cut(s) 29
Hpy188I TCNGA 1 cut(s) 223
Hpy188III TCNNGA 3 cut(s) 19, 200, 251
Hpy8I GTNNAC 1 cut(s) 29
Hpy99I CGWCG 1 cut(s) 164
HpyCH4III ACNGT 1 cut(s) 130
HpyCH4V TGCA 3 cut(s) 228, 284, 365
HpyF10VI GCNNNNNNNGC 1 cut(s) 56
Hsp92I GRCGYC 1 cut(s) 373
Hsp92II CATG 1 cut(s) 214
HspAI GCGC 1 cut(s) 373
KasI GGCGCC 1 cut(s) 372
Kpn2I TCCGGA 1 cut(s) 199
Kzo9I GATC 2 cut(s) 123, 196
LpnPI CCDG 8 cut(s) 131, 200, 213, 249, 294, 319, 365, 405
Lsp1109I GCAGC 4 cut(s) 59, 240, 349, 367
LweI GCATC 1 cut(s) 120
MaeIII GTNAC 1 cut(s) 299
MalI GATC 2 cut(s) 125, 198
MboI GATC 2 cut(s) 123, 196
MboII GAAGA 1 cut(s) 246
MflI RGATCY 1 cut(s) 196
MluCI AATT 2 cut(s) 254, 402
Mly113I GGCGCC 1 cut(s) 373
MlyI GAGTC 1 cut(s) 212
MnlI CCTC 6 cut(s) 46, 145, 164, 327, 335, 363
MroI TCCGGA 1 cut(s) 199
MseI TTAA 2 cut(s) 275, 424
MspA1I CMGCKG 1 cut(s) 44
MspI CCGG 1 cut(s) 200
Mva1269I GAATGC 1 cut(s) 230
MwoI GCNNNNNNNGC 1 cut(s) 56
NarI GGCGCC 1 cut(s) 373
NdeII GATC 2 cut(s) 123, 196
NlaIII CATG 1 cut(s) 214
NlaIV GGNNCC 2 cut(s) 198, 374
NmuCI GTSAC 1 cut(s) 299
PcsI WCGNNNNNNNCGW 1 cut(s) 293
PctI GAATGC 1 cut(s) 230
PfeI GAWTC 1 cut(s) 237
PflFI GACNNNGTC 1 cut(s) 82
PkrI GCNGC 5 cut(s) 46, 49, 230, 357, 364
PleI GAGTC 1 cut(s) 212
PluTI GGCGCC 1 cut(s) 376
PpsI GAGTC 1 cut(s) 212
PspFI CCCAGC 1 cut(s) 351
PspN4I GGNNCC 2 cut(s) 198, 374
PsuI RGATCY 1 cut(s) 196
PsyI GACNNNGTC 1 cut(s) 82
SaqAI TTAA 2 cut(s) 275, 424
SatI GCNGC 5 cut(s) 45, 48, 229, 356, 363
Sau3AI GATC 2 cut(s) 123, 196
SchI GAGTC 1 cut(s) 212
SetI ASST 2 cut(s) 52, 275
SfaNI GCATC 1 cut(s) 120
SfoI GGCGCC 1 cut(s) 374
Sse9I AATT 2 cut(s) 254, 402
SsiI CCGC 2 cut(s) 44, 396
SspDI GGCGCC 1 cut(s) 372
TaaI ACNGT 1 cut(s) 130
TaqII GACCGA 1 cut(s) 318
TasI AATT 2 cut(s) 254, 402
TauI GCSGC 1 cut(s) 47
TfiI GAWTC 1 cut(s) 237
Tru1I TTAA 2 cut(s) 275, 424
Tru9I TTAA 2 cut(s) 275, 424
TscAI CASTG 1 cut(s) 334
TseFI GTSAC 1 cut(s) 299
TseI GCWGC 4 cut(s) 47, 228, 355, 362
Tsp45I GTSAC 1 cut(s) 299
TspDTI ATGAA 1 cut(s) 199
TspGWI ACGGA 1 cut(s) 309
TspRI CASTG 1 cut(s) 334
Tth111I GACNNNGTC 1 cut(s) 82
XapI RAATTY 1 cut(s) 254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.