Rorug07G0104900

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0000549
Physical Location & Seq
Forward (+)
8237858 .. 8240064
2207 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0104900.1

Sequence Viewer

Length: 861 bp
ATGTCCACCGAGGAGAACTTGTTTCTTAGCAGTGTGTTTCAAAGAAAAGATGTATCCCTTGACTTTGCAGTCCCAAGCAACAAGCGTGTGATCAGAATATCGCACGCTAAGCTGACTGGAACCTGCTCTGGTGATGGCCTTACAGACATTGCCTATCATGATATATGTACTCTAGCGGACCATCAAGTTATTGTCTCAGTCCCTGACTTCCTGCTTTTATCCGAAAGGCAATGTTATACAGAGAAGATGGCTGAAATTCTTACCAAATTGAAGGTCCCAGAATTTGAGCAACCACCTATCATTGATAAAATATTTATGGAGGTTGAACTGGCTGACAACCCTGAGTGGCCTGTTGCTTGCTTTATAAAGGATATCACCTTATTTCATCAGACGATCAACCTGGCCTATCAGTTAGAGAGTTTCGAGAAAGTGAGAATTGATAGTTTGGAAGATACTGTTAGACAGCAACAATGTGTCATTTGTAAGGAGAGCCTTGATCACTTTGAAGGTGTAGAAGAAGGGATTGATCGTGGTCAGCTGATGATTATTCACTTGTCCTGCTCACATCTTTATCACGAAGATTGCATTTTCCAATGGCTGGCGAAGAGTCAGTTGTGTCCCTTGTGTCGACACTCAATGGCAGTTGTGGAACAGGCGACGCCGGTTGTTGAACAGGCGACGCCGGTTGTGGAACAGGCAATTCTGGTTGTTGAACAGGCGATGCCGGTTGTGGAACAGGCAGCTGAGCCATCGAAACCGTCATGGTGGCATTTGCATTGGCCTATGCTGCTCACAGCATCTGCGGGTGGTATATTAACGGCTATGCTGGTATGTAAATTATTGAAGCAAAGGCAAGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04145 ko04210 ko04530 ko04540 ko05130 map04145 map04210 map04530 map04540 map05130
Pfam Domains
Protein Families
Tubulin Tubulin_C

Protein Analysis

286

Amino Acids

32.41

Weight (kDa)

5.05

Isoelectric Point (pI)

44.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 156 - 210 1.3e-08 Ring finger domain
zf-rbx1 PF12678 157 - 210 6.2e-10 RING-H2 zinc finger domain
zf-C3HC4_2 PF13923 186 - 209 5.1e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000549)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04820 AT1G50010 AT4G14960 AT4G14960
fragaria_vesca FvH4_1g18660 FvH4_2g03140 FvH4_5g33560 FvH4_6g48940 FvH4_6g48940
malus_domestica MD02G1204800.v1.1 MD05G1106800.v1.1 MD08G1210600.v1.1 MD09G1049500.v1.1 MD10G1111600.v1.1 MD15G1398700.v1.1 MD17G1049700.v1.1
prunus_persica Prupe.1G542600_v2.0.a1 Prupe.3G269300_v2.0.a1 Prupe.6G212700_v2.0.a1 Prupe.8G150900_v2.0.a1
pyrus_communis pycom05g10370 pycom08g18150 pycom10g09640 pycom10g09660 pycom111g04050 pycom15g35470 pycom17g04460
rosa_chinensis RchiOBHm_Chr2g0109481 RchiOBHm_Chr2g0168751 RchiOBHm_Chr3g0453071 RchiOBHm_Chr3g0460741 RchiOBHm_Chr3g0467971 RchiOBHm_Chr4g0400901 RchiOBHm_Chr6g0248011 RchiOBHm_Chr7g0232381 RchiOBHm_Chr7g0238121
rosa_laevigata RLG00000001350 RLG00000009167 RLG00000015163 RLG00000017708 RLG00000017741 RLG00000021807 RLG00000023849 RLG00000026518
rosa_multiflora Rmu_sc0000332.1_g000021 Rmu_sc0000332.1_g000029 Rmu_sc0002416.1_g000007 Rmu_sc0002611.1_g000001 Rmu_sc0003113.1_g000016 Rmu_sc0004780.1_g000001 Rmu_sc0004805.1_g000035 Rmu_sc0010633.1_g000010 Rmu_sc0011069.1_g000003 Rmu_sc0016170.1_g000002 Rmu_sc0025757.1_g000003 Rmu_ssc0000484.1_g000003
rosa_roxburghii Rroxscaffold_2G00134200 Rroxscaffold_4G00321030 Rroxscaffold_7G00213500
rosa_rugosa Rorug01G0283100 Rorug02G0160500 Rorug02G0535300 Rorug04G0027000 Rorug04G0328100 Rorug05G0388700 Rorug05G0530200 Rorug06G0489200 Rorug07G0104900 Rorug07G0209400 Rorug07G0274500
rosa_samantha Rh2BG222800 Rh2BG618900 Rh2CG214400 Rh2CG587200 Rh6BG040200 Rh6CG206500 Rh6DG035600 Rh7BG401600 Rh7BG401700 Rh7DG418400
rosa_wichuraiana Rw2G050240 Rw6G003920 Rw7G035430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 365
AasI GACNNNNNNGTC 1 cut(s) 68
Acc36I ACCTGC 1 cut(s) 131
AccB7I CCANNNNNTGG 1 cut(s) 598
AccI GTMKAC 1 cut(s) 628
AciI CCGC 2 cut(s) 176, 803
AcsI RAATTY 2 cut(s) 255, 281
AcyI GRCGYC 2 cut(s) 659, 680
AfaI GTAC 1 cut(s) 169
AfiI CCNNNNNNNGG 1 cut(s) 598
AgsI TTSAA 7 cut(s) 41, 271, 326, 506, 671, 713, 844
AjnI CCWGG 1 cut(s) 399
AluBI AGCT 3 cut(s) 112, 538, 743
AluI AGCT 3 cut(s) 112, 538, 743
Alw26I GTCTC 1 cut(s) 199
AlwNI CAGNNNCTG 2 cut(s) 203, 800
AoxI GGCC 4 cut(s) 136, 347, 402, 779
ApeKI GCWGC 2 cut(s) 740, 787
ApoI RAATTY 2 cut(s) 255, 281
ArsI GACNNNNNNTTYG 2 cut(s) 258, 290
AspS9I GGNCC 2 cut(s) 178, 274
AsuHPI GGTGA 2 cut(s) 143, 367
AvaII GGWCC 2 cut(s) 178, 274
BbvI GCAGC 2 cut(s) 752, 774
BccI CCATC 4 cut(s) 128, 189, 241, 757
BceAI ACGGC 1 cut(s) 834
BciT130I CCWGG 1 cut(s) 401
BciVI GTATCC 1 cut(s) 64
BclI TGATCA 2 cut(s) 90, 496
BcoDI GTCTC 1 cut(s) 199
BfaI CTAG 1 cut(s) 173
BfuAI ACCTGC 1 cut(s) 131
BfuI GTATCC 1 cut(s) 64
BisI GCNGC 2 cut(s) 741, 788
BlpI GCTNAGC 2 cut(s) 108, 744
BlsI GCNGC 2 cut(s) 742, 789
Bme1390I CCNGG 1 cut(s) 401
Bme18I GGWCC 2 cut(s) 178, 274
BmgT120I GGNCC 2 cut(s) 178, 274
BmiI GGNNCC 2 cut(s) 121, 276
BmrFI CCNGG 1 cut(s) 401
BmsI GCATC 2 cut(s) 711, 806
Bpu1102I GCTNAGC 2 cut(s) 108, 744
BsaHI GRCGYC 2 cut(s) 659, 680
BsaJI CCNNGG 1 cut(s) 9
BsaXI ACNNNNNCTCC 2 cut(s) 5, 35
Bsc4I CCNNNNNNNGG 1 cut(s) 598
Bse118I RCCGGY 3 cut(s) 661, 682, 724
Bse1I ACTGG 2 cut(s) 121, 333
Bse3DI GCAATG 2 cut(s) 147, 236
BseBI CCWGG 1 cut(s) 401
BseDI CCNNGG 1 cut(s) 9
BseLI CCNNNNNNNGG 1 cut(s) 598
BseMI GCAATG 2 cut(s) 147, 236
BseMII CTCAG 3 cut(s) 210, 333, 735
BseNI ACTGG 2 cut(s) 121, 333
BseRI GAGGAG 1 cut(s) 26
BseXI GCAGC 2 cut(s) 752, 774
BshFI GGCC 4 cut(s) 138, 349, 404, 781
BsiSI CCGG 3 cut(s) 662, 683, 725
BslFI GGGAC 4 cut(s) 56, 185, 260, 603
BslI CCNNNNNNNGG 1 cut(s) 598
BsmAI GTCTC 1 cut(s) 199
BsmFI GGGAC 4 cut(s) 56, 185, 260, 603
BsnI GGCC 4 cut(s) 138, 349, 404, 781
Bsp143I GATC 4 cut(s) 90, 393, 496, 526
Bsp1720I GCTNAGC 2 cut(s) 108, 744
BspACI CCGC 2 cut(s) 176, 803
BspANI GGCC 4 cut(s) 138, 349, 404, 781
BspCNI CTCAG 3 cut(s) 209, 334, 736
BspHI TCATGA 1 cut(s) 157
BspLI GGNNCC 2 cut(s) 121, 276
BspMI ACCTGC 1 cut(s) 131
BsrDI GCAATG 2 cut(s) 147, 236
BsrFI RCCGGY 3 cut(s) 661, 682, 724
BsrI ACTGG 2 cut(s) 121, 333
BssAI RCCGGY 3 cut(s) 661, 682, 724
BssECI CCNNGG 1 cut(s) 9
BssMI GATC 4 cut(s) 90, 393, 496, 526
BssNI GRCGYC 2 cut(s) 659, 680
Bst2UI CCWGG 1 cut(s) 401
Bst4CI ACNGT 2 cut(s) 457, 759
Bst6I CTCTTC 1 cut(s) 599
BstACI GRCGYC 2 cut(s) 659, 680
BstC8I GCNNGC 3 cut(s) 105, 358, 600
BstDEI CTNAG 5 cut(s) 26, 108, 196, 342, 744
BstKTI GATC 4 cut(s) 93, 396, 499, 529
BstMAI GTCTC 1 cut(s) 199
BstMBI GATC 4 cut(s) 90, 393, 496, 526
BstMWI GCNNNNNNNGC 2 cut(s) 109, 787
BstNI CCWGG 1 cut(s) 401
BstSCI CCNGG 1 cut(s) 399
BstV1I GCAGC 2 cut(s) 752, 774
BsuI GTATCC 1 cut(s) 64
BsuRI GGCC 4 cut(s) 138, 349, 404, 781
BtgZI GCGATG 1 cut(s) 734
BtsI GCAGTG 1 cut(s) 37
BtsIMutI CAGTG 1 cut(s) 37
BveI ACCTGC 1 cut(s) 131
Cac8I GCNNGC 3 cut(s) 105, 358, 600
CaiI CAGNNNCTG 2 cut(s) 203, 800
CciI TCATGA 1 cut(s) 157
Cfr10I RCCGGY 3 cut(s) 661, 682, 724
Cfr13I GGNCC 2 cut(s) 178, 274
CseI GACGC 2 cut(s) 667, 688
Csp6I GTAC 1 cut(s) 168
CviAII CATG 2 cut(s) 158, 762
CviQI GTAC 1 cut(s) 168
DdeI CTNAG 5 cut(s) 26, 108, 196, 342, 744
DpnI GATC 4 cut(s) 92, 395, 498, 528
DpnII GATC 4 cut(s) 90, 393, 496, 526
DrdI GACNNNNNNGTC 1 cut(s) 68
DseDI GACNNNNNNGTC 1 cut(s) 68
Eam1104I CTCTTC 1 cut(s) 599
EarI CTCTTC 1 cut(s) 599
Eco32I GATATC 1 cut(s) 373
Eco47I GGWCC 2 cut(s) 178, 274
EcoO109I RGGNCCY 1 cut(s) 274
EcoRII CCWGG 1 cut(s) 399
EcoRV GATATC 1 cut(s) 373
FaeI CATG 2 cut(s) 161, 765
FaqI GGGAC 4 cut(s) 56, 185, 260, 603
FatI CATG 2 cut(s) 157, 761
FauI CCCGC 1 cut(s) 796
FbaI TGATCA 2 cut(s) 90, 496
FblI GTMKAC 1 cut(s) 628
Fnu4HI GCNGC 2 cut(s) 741, 788
Fsp4HI GCNGC 2 cut(s) 741, 788
FspBI CTAG 1 cut(s) 173
GluI GCNGC 2 cut(s) 741, 788
HaeIII GGCC 4 cut(s) 138, 349, 404, 781
HapII CCGG 3 cut(s) 662, 683, 725
HgaI GACGC 2 cut(s) 667, 688
Hin1I GRCGYC 2 cut(s) 659, 680
Hin1II CATG 2 cut(s) 161, 765
HincII GTYRAC 1 cut(s) 629
HindII GTYRAC 1 cut(s) 629
HinfI GANTC 1 cut(s) 607
HpaII CCGG 3 cut(s) 662, 683, 725
HphI GGTGA 2 cut(s) 143, 367
Hpy166II GTNNAC 2 cut(s) 6, 629
Hpy188I TCNGA 3 cut(s) 95, 223, 390
Hpy188III TCNNGA 3 cut(s) 158, 424, 575
Hpy8I GTNNAC 2 cut(s) 6, 629
Hpy99I CGWCG 2 cut(s) 661, 682
HpyAV CCTTC 3 cut(s) 265, 500, 512
HpyCH4III ACNGT 2 cut(s) 457, 759
HpyCH4V TGCA 3 cut(s) 68, 585, 775
HpyF10VI GCNNNNNNNGC 2 cut(s) 109, 787
HpyF3I CTNAG 5 cut(s) 26, 108, 196, 342, 744
Hsp92I GRCGYC 2 cut(s) 659, 680
Hsp92II CATG 2 cut(s) 161, 765
Ksp22I TGATCA 2 cut(s) 90, 496
Kzo9I GATC 4 cut(s) 90, 393, 496, 526
Lsp1109I GCAGC 2 cut(s) 752, 774
LweI GCATC 2 cut(s) 711, 806
MaeI CTAG 1 cut(s) 173
MalI GATC 4 cut(s) 92, 395, 498, 528
MboI GATC 4 cut(s) 90, 393, 496, 526
MboII GAAGA 5 cut(s) 256, 461, 527, 590, 616
MluCI AATT 6 cut(s) 255, 266, 281, 435, 699, 836
MlyI GAGTC 1 cut(s) 616
MnlI CCTC 2 cut(s) 4, 313
MseI TTAA 1 cut(s) 815
MspA1I CMGCKG 2 cut(s) 538, 743
MspI CCGG 3 cut(s) 662, 683, 725
MspR9I CCNGG 1 cut(s) 401
MvaI CCWGG 1 cut(s) 401
MwoI GCNNNNNNNGC 2 cut(s) 109, 787
NdeII GATC 4 cut(s) 90, 393, 496, 526
NlaIII CATG 2 cut(s) 161, 765
NlaIV GGNNCC 2 cut(s) 121, 276
PagI TCATGA 1 cut(s) 157
PflMI CCANNNNNTGG 1 cut(s) 598
PkrI GCNGC 2 cut(s) 742, 789
PleI GAGTC 1 cut(s) 615
PpsI GAGTC 1 cut(s) 615
PpuMI RGGWCCY 1 cut(s) 274
PsiI TTATAA 1 cut(s) 365
Psp5II RGGWCCY 1 cut(s) 274
Psp6I CCWGG 1 cut(s) 399
PspGI CCWGG 1 cut(s) 399
PspN4I GGNNCC 2 cut(s) 121, 276
PspPI GGNCC 2 cut(s) 178, 274
PspPPI RGGWCCY 1 cut(s) 274
PstNI CAGNNNCTG 2 cut(s) 203, 800
PvuII CAGCTG 2 cut(s) 538, 743
RsaI GTAC 1 cut(s) 169
RsaNI GTAC 1 cut(s) 168
SalI GTCGAC 1 cut(s) 627
SaqAI TTAA 1 cut(s) 815
SatI GCNGC 2 cut(s) 741, 788
Sau3AI GATC 4 cut(s) 90, 393, 496, 526
Sau96I GGNCC 2 cut(s) 178, 274
SchI GAGTC 1 cut(s) 616
ScrFI CCNGG 1 cut(s) 401
SfaNI GCATC 2 cut(s) 711, 806
SinI GGWCC 2 cut(s) 178, 274
Sse9I AATT 6 cut(s) 255, 266, 281, 435, 699, 836
SsiI CCGC 2 cut(s) 176, 803
SspI AATATT 1 cut(s) 312
SspMI CTAG 1 cut(s) 173
StyD4I CCNGG 1 cut(s) 399
TaaI ACNGT 2 cut(s) 457, 759
TaqI TCGA 3 cut(s) 423, 628, 752
TasI AATT 6 cut(s) 255, 266, 281, 435, 699, 836
TatI WGTACW 1 cut(s) 167
Tru1I TTAA 1 cut(s) 815
Tru9I TTAA 1 cut(s) 815
TscAI CASTG 1 cut(s) 37
TseI GCWGC 2 cut(s) 740, 787
TspDTI ATGAA 1 cut(s) 374
TspRI CASTG 1 cut(s) 37
Van91I CCANNNNNTGG 1 cut(s) 598
VpaK11BI GGWCC 2 cut(s) 178, 274
XapI RAATTY 2 cut(s) 255, 281
XmiI GTMKAC 1 cut(s) 628
XspI CTAG 1 cut(s) 173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.