Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 242 |
| AccB1I | GGYRCC | 3 cut(s) | 242, 737, 1038 |
| AccIII | TCCGGA | 1 cut(s) | 366 |
| AciI | CCGC | 5 cut(s) | 250, 282, 361, 833, 1059 |
| AclWI | GGATC | 1 cut(s) | 1278 |
| AcoI | YGGCCR | 1 cut(s) | 100 |
| AcsI | RAATTY | 1 cut(s) | 761 |
| AcuI | CTGAAG | 3 cut(s) | 74, 142, 1278 |
| AfaI | GTAC | 4 cut(s) | 244, 845, 956, 1349 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 748, 926, 1108 |
| AgsI | TTSAA | 3 cut(s) | 398, 415, 1054 |
| AjnI | CCWGG | 4 cut(s) | 42, 109, 582, 1090 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1235, 1267 |
| AluBI | AGCT | 4 cut(s) | 67, 173, 256, 499 |
| AluI | AGCT | 4 cut(s) | 67, 173, 256, 499 |
| Alw21I | GWGCWC | 2 cut(s) | 609, 1218 |
| Alw26I | GTCTC | 2 cut(s) | 535, 699 |
| Alw44I | GTGCAC | 1 cut(s) | 1214 |
| AlwI | GGATC | 1 cut(s) | 1278 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 866, 1316 |
| Ama87I | CYCGRG | 1 cut(s) | 209 |
| Aor13HI | TCCGGA | 1 cut(s) | 366 |
| AoxI | GGCC | 4 cut(s) | 33, 85, 100, 939 |
| ApaLI | GTGCAC | 1 cut(s) | 1214 |
| ApeKI | GCWGC | 3 cut(s) | 296, 989, 1277 |
| ApoI | RAATTY | 1 cut(s) | 761 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1166, 1198 |
| Asp718I | GGTACC | 1 cut(s) | 242 |
| AspLEI | GCGC | 3 cut(s) | 645, 1017, 1310 |
| AspS9I | GGNCC | 3 cut(s) | 19, 358, 1111 |
| AsuHPI | GGTGA | 5 cut(s) | 125, 146, 865, 974, 1241 |
| AvaI | CYCGRG | 1 cut(s) | 209 |
| AvaII | GGWCC | 3 cut(s) | 19, 358, 1111 |
| BaeGI | GKGCMC | 4 cut(s) | 742, 975, 1043, 1218 |
| BalI | TGGCCA | 1 cut(s) | 102 |
| BanI | GGYRCC | 3 cut(s) | 242, 737, 1038 |
| BanII | GRGCYC | 3 cut(s) | 216, 552, 894 |
| BauI | CACGAG | 1 cut(s) | 189 |
| Bbv12I | GWGCWC | 2 cut(s) | 609, 1218 |
| BbvCI | CCTCAGC | 2 cut(s) | 1155, 1320 |
| BbvI | GCAGC | 3 cut(s) | 283, 976, 1264 |
| BccI | CCATC | 5 cut(s) | 92, 526, 728, 898, 1328 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 170, 204 |
| BciT130I | CCWGG | 4 cut(s) | 44, 111, 584, 1092 |
| BciVI | GTATCC | 2 cut(s) | 50, 525 |
| BcoDI | GTCTC | 2 cut(s) | 535, 699 |
| BfmI | CTRYAG | 1 cut(s) | 637 |
| BfoI | RGCGCY | 1 cut(s) | 646 |
| BfuI | GTATCC | 2 cut(s) | 50, 525 |
| BisI | GCNGC | 4 cut(s) | 283, 297, 990, 1278 |
| BlsI | GCNGC | 4 cut(s) | 284, 298, 991, 1279 |
| BmcAI | AGTACT | 1 cut(s) | 1349 |
| Bme1390I | CCNGG | 4 cut(s) | 44, 111, 584, 1092 |
| Bme18I | GGWCC | 3 cut(s) | 19, 358, 1111 |
| BmeT110I | CYCGRG | 1 cut(s) | 209 |
| BmgT120I | GGNCC | 3 cut(s) | 19, 358, 1111 |
| BmiI | GGNNCC | 5 cut(s) | 244, 739, 780, 1040, 1089 |
| BmrFI | CCNGG | 4 cut(s) | 44, 111, 584, 1092 |
| BmrI | ACTGGG | 1 cut(s) | 399 |
| BmsI | GCATC | 3 cut(s) | 130, 283, 372 |
| BmuI | ACTGGG | 1 cut(s) | 399 |
| BoxI | GACNNNNGTC | 2 cut(s) | 921, 1148 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 842, 874, 1148, 1180 |
| BpmI | CTGGAG | 1 cut(s) | 189 |
| Bpu10I | CCTNAGC | 3 cut(s) | 267, 1155, 1320 |
| BpuEI | CTTGAG | 2 cut(s) | 98, 1304 |
| BsaI | GGTCTC | 1 cut(s) | 535 |
| BsaJI | CCNNGG | 4 cut(s) | 313, 958, 975, 1107 |
| BsaWI | WCCGGW | 2 cut(s) | 366, 1046 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 827, 857 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 748, 926, 1108 |
| Bse118I | RCCGGY | 1 cut(s) | 35 |
| Bse1I | ACTGG | 7 cut(s) | 172, 244, 394, 439, 1040, 1146, 1223 |
| Bse3DI | GCAATG | 1 cut(s) | 58 |
| BseAI | TCCGGA | 1 cut(s) | 366 |
| BseBI | CCWGG | 4 cut(s) | 44, 111, 584, 1092 |
| BseDI | CCNNGG | 4 cut(s) | 313, 958, 975, 1107 |
| BseGI | GGATG | 5 cut(s) | 363, 757, 896, 985, 1336 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 748, 926, 1108 |
| BseMI | GCAATG | 1 cut(s) | 58 |
| BseMII | CTCAG | 6 cut(s) | 258, 710, 716, 873, 1146, 1311 |
| BseNI | ACTGG | 7 cut(s) | 172, 244, 394, 439, 1040, 1146, 1223 |
| BseSI | GKGCMC | 4 cut(s) | 742, 975, 1043, 1218 |
| BseXI | GCAGC | 3 cut(s) | 283, 976, 1264 |
| BseYI | CCCAGC | 1 cut(s) | 60 |
| BsgI | GTGCAG | 1 cut(s) | 370 |
| BshFI | GGCC | 4 cut(s) | 35, 87, 102, 941 |
| BshNI | GGYRCC | 3 cut(s) | 242, 737, 1038 |
| BsiHKAI | GWGCWC | 2 cut(s) | 609, 1218 |
| BsiHKCI | CYCGRG | 1 cut(s) | 209 |
| BsiSI | CCGG | 3 cut(s) | 36, 367, 1047 |
| BslFI | GGGAC | 1 cut(s) | 445 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 748, 926, 1108 |
| BsmAI | GTCTC | 2 cut(s) | 535, 699 |
| BsmFI | GGGAC | 1 cut(s) | 445 |
| BsmI | GAATGC | 1 cut(s) | 991 |
| BsnI | GGCC | 4 cut(s) | 35, 87, 102, 941 |
| Bso31I | GGTCTC | 1 cut(s) | 535 |
| BsoBI | CYCGRG | 1 cut(s) | 209 |
| Bsp1286I | GDGCHC | 8 cut(s) | 216, 552, 609, 742, 894, 975, 1043, 1218 |
| Bsp13I | TCCGGA | 1 cut(s) | 366 |
| Bsp143I | GATC | 5 cut(s) | 205, 346, 1131, 1186, 1270 |
| BspACI | CCGC | 5 cut(s) | 250, 282, 361, 833, 1059 |
| BspANI | GGCC | 4 cut(s) | 35, 87, 102, 941 |
| BspCNI | CTCAG | 6 cut(s) | 259, 709, 717, 872, 1147, 1312 |
| BspEI | TCCGGA | 1 cut(s) | 366 |
| BspLI | GGNNCC | 5 cut(s) | 244, 739, 780, 1040, 1089 |
| BspMAI | CTGCAG | 1 cut(s) | 641 |
| BspPI | GGATC | 1 cut(s) | 1278 |
| BspQI | GCTCTTC | 1 cut(s) | 263 |
| BspT107I | GGYRCC | 3 cut(s) | 242, 737, 1038 |
| BspTNI | GGTCTC | 1 cut(s) | 535 |
| BsrDI | GCAATG | 1 cut(s) | 58 |
| BsrFI | RCCGGY | 1 cut(s) | 35 |
| BsrI | ACTGG | 7 cut(s) | 172, 244, 394, 439, 1040, 1146, 1223 |
| BssAI | RCCGGY | 1 cut(s) | 35 |
| BssECI | CCNNGG | 4 cut(s) | 313, 958, 975, 1107 |
| BssMI | GATC | 5 cut(s) | 205, 346, 1131, 1186, 1270 |
| BssSI | CACGAG | 1 cut(s) | 189 |
| BssT1I | CCWWGG | 2 cut(s) | 975, 1107 |
| Bst2BI | CACGAG | 1 cut(s) | 189 |
| Bst2UI | CCWGG | 4 cut(s) | 44, 111, 584, 1092 |
| Bst4CI | ACNGT | 8 cut(s) | 124, 220, 511, 560, 686, 881, 959, 1084 |
| Bst6I | CTCTTC | 1 cut(s) | 263 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 57, 1316 |
| BstC8I | GCNNGC | 7 cut(s) | 58, 175, 179, 254, 641, 943, 1206 |
| BstDEI | CTNAG | 6 cut(s) | 267, 696, 725, 859, 1155, 1320 |
| BstDSI | CCRYGG | 2 cut(s) | 313, 958 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1106 |
| BstF5I | GGATG | 5 cut(s) | 363, 757, 896, 985, 1336 |
| BstH2I | RGCGCY | 1 cut(s) | 646 |
| BstHHI | GCGC | 3 cut(s) | 645, 1017, 1310 |
| BstKTI | GATC | 5 cut(s) | 208, 349, 1134, 1189, 1273 |
| BstMAI | GTCTC | 2 cut(s) | 535, 699 |
| BstMBI | GATC | 5 cut(s) | 205, 346, 1131, 1186, 1270 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 57, 360, 839, 995, 1205, 1316 |
| BstNI | CCWGG | 4 cut(s) | 44, 111, 584, 1092 |
| BstNSI | RCATGY | 1 cut(s) | 185 |
| BstPAI | GACNNNNGTC | 2 cut(s) | 921, 1148 |
| BstSCI | CCNGG | 4 cut(s) | 42, 109, 582, 1090 |
| BstSFI | CTRYAG | 1 cut(s) | 637 |
| BstSLI | GKGCMC | 4 cut(s) | 742, 975, 1043, 1218 |
| BstV1I | GCAGC | 3 cut(s) | 283, 976, 1264 |
| BstX2I | RGATCY | 1 cut(s) | 1270 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 110 |
| BstYI | RGATCY | 1 cut(s) | 1270 |
| BsuI | GTATCC | 2 cut(s) | 50, 525 |
| BsuRI | GGCC | 4 cut(s) | 35, 87, 102, 941 |
| BtgI | CCRYGG | 2 cut(s) | 313, 958 |
| BtsCI | GGATG | 5 cut(s) | 363, 757, 896, 985, 1336 |
| BtsI | GCAGTG | 1 cut(s) | 609 |
| Cac8I | GCNNGC | 7 cut(s) | 58, 175, 179, 254, 641, 943, 1206 |
| CaiI | CAGNNNCTG | 2 cut(s) | 866, 1316 |
| CfoI | GCGC | 3 cut(s) | 645, 1017, 1310 |
| Cfr10I | RCCGGY | 1 cut(s) | 35 |
| Cfr13I | GGNCC | 3 cut(s) | 19, 358, 1111 |
| Csp6I | GTAC | 4 cut(s) | 243, 844, 955, 1348 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 205, 240 |
| CviAII | CATG | 8 cut(s) | 83, 182, 848, 901, 926, 937, 1129, 1192 |
| CviQI | GTAC | 4 cut(s) | 243, 844, 955, 1348 |
| DdeI | CTNAG | 6 cut(s) | 267, 696, 725, 859, 1155, 1320 |
| DpnI | GATC | 5 cut(s) | 207, 348, 1133, 1188, 1272 |
| DpnII | GATC | 5 cut(s) | 205, 346, 1131, 1186, 1270 |
| EaeI | YGGCCR | 1 cut(s) | 100 |
| Eam1104I | CTCTTC | 1 cut(s) | 263 |
| EarI | CTCTTC | 1 cut(s) | 263 |
| Eco130I | CCWWGG | 2 cut(s) | 975, 1107 |
| Eco24I | GRGCYC | 3 cut(s) | 216, 552, 894 |
| Eco31I | GGTCTC | 1 cut(s) | 535 |
| Eco32I | GATATC | 1 cut(s) | 634 |
| Eco47I | GGWCC | 3 cut(s) | 19, 358, 1111 |
| Eco57I | CTGAAG | 3 cut(s) | 74, 142, 1278 |
| Eco88I | CYCGRG | 1 cut(s) | 209 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1106 |
| EcoRI | GAATTC | 1 cut(s) | 761 |
| EcoRII | CCWGG | 4 cut(s) | 42, 109, 582, 1090 |
| EcoRV | GATATC | 1 cut(s) | 634 |
| EcoT14I | CCWWGG | 2 cut(s) | 975, 1107 |
| EcoT38I | GRGCYC | 3 cut(s) | 216, 552, 894 |
| ErhI | CCWWGG | 2 cut(s) | 975, 1107 |
| FaeI | CATG | 8 cut(s) | 86, 185, 851, 904, 929, 940, 1132, 1195 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 390, 422, 1193, 1225 |
| FaqI | GGGAC | 1 cut(s) | 445 |
| FatI | CATG | 8 cut(s) | 82, 181, 847, 900, 925, 936, 1128, 1191 |
| FauNDI | CATATG | 1 cut(s) | 814 |
| Fnu4HI | GCNGC | 4 cut(s) | 283, 297, 990, 1278 |
| FokI | GGATG | 5 cut(s) | 350, 764, 883, 992, 1343 |
| FriOI | GRGCYC | 3 cut(s) | 216, 552, 894 |
| Fsp4HI | GCNGC | 4 cut(s) | 283, 297, 990, 1278 |
| GlaI | GCGC | 3 cut(s) | 644, 1016, 1309 |
| GluI | GCNGC | 4 cut(s) | 283, 297, 990, 1278 |
| GsaI | CCCAGC | 1 cut(s) | 64 |
| GsuI | CTGGAG | 1 cut(s) | 189 |
| HaeII | RGCGCY | 1 cut(s) | 646 |
| HaeIII | GGCC | 4 cut(s) | 35, 87, 102, 941 |
| HapII | CCGG | 3 cut(s) | 36, 367, 1047 |
| HhaI | GCGC | 3 cut(s) | 645, 1017, 1310 |
| Hin1II | CATG | 8 cut(s) | 86, 185, 851, 904, 929, 940, 1132, 1195 |
| Hin6I | GCGC | 3 cut(s) | 643, 1015, 1308 |
| HinP1I | GCGC | 3 cut(s) | 643, 1015, 1308 |
| HindIII | AAGCTT | 1 cut(s) | 497 |
| HinfI | GANTC | 3 cut(s) | 792, 1050, 1313 |
| HpaII | CCGG | 3 cut(s) | 36, 367, 1047 |
| HphI | GGTGA | 5 cut(s) | 125, 146, 865, 974, 1241 |
| Hpy166II | GTNNAC | 4 cut(s) | 22, 507, 1033, 1216 |
| Hpy188I | TCNGA | 3 cut(s) | 726, 951, 1258 |
| Hpy188III | TCNNGA | 6 cut(s) | 209, 367, 650, 766, 1114, 1167 |
| Hpy8I | GTNNAC | 4 cut(s) | 22, 507, 1033, 1216 |
| HpyAV | CCTTC | 3 cut(s) | 98, 832, 1238 |
| HpyCH4III | ACNGT | 8 cut(s) | 124, 220, 511, 560, 686, 881, 959, 1084 |
| HpyCH4IV | ACGT | 1 cut(s) | 466 |
| HpyCH4V | TGCA | 7 cut(s) | 12, 387, 602, 639, 818, 1128, 1216 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 57, 360, 839, 995, 1205, 1316 |
| HpyF3I | CTNAG | 6 cut(s) | 267, 696, 725, 859, 1155, 1320 |
| HpySE526I | ACGT | 1 cut(s) | 466 |
| Hsp92II | CATG | 8 cut(s) | 86, 185, 851, 904, 929, 940, 1132, 1195 |
| HspAI | GCGC | 3 cut(s) | 643, 1015, 1308 |
| Kpn2I | TCCGGA | 1 cut(s) | 366 |
| KpnI | GGTACC | 1 cut(s) | 246 |
| Kzo9I | GATC | 5 cut(s) | 205, 346, 1131, 1186, 1270 |
| LguI | GCTCTTC | 1 cut(s) | 263 |
| LmnI | GCTCC | 3 cut(s) | 64, 170, 612 |
| Lsp1109I | GCAGC | 3 cut(s) | 283, 976, 1264 |
| LweI | GCATC | 3 cut(s) | 130, 283, 372 |
| MaeII | ACGT | 1 cut(s) | 466 |
| MaeIII | GTNAC | 4 cut(s) | 113, 317, 754, 914 |
| MalI | GATC | 5 cut(s) | 207, 348, 1133, 1188, 1272 |
| MboI | GATC | 5 cut(s) | 205, 346, 1131, 1186, 1270 |
| MboII | GAAGA | 4 cut(s) | 148, 250, 302, 1310 |
| MflI | RGATCY | 1 cut(s) | 1270 |
| MhlI | GDGCHC | 8 cut(s) | 216, 552, 609, 742, 894, 975, 1043, 1218 |
| MlsI | TGGCCA | 1 cut(s) | 102 |
| MluCI | AATT | 2 cut(s) | 761, 1170 |
| MluNI | TGGCCA | 1 cut(s) | 102 |
| MlyI | GAGTC | 1 cut(s) | 1322 |
| Mox20I | TGGCCA | 1 cut(s) | 102 |
| MroI | TCCGGA | 1 cut(s) | 366 |
| MscI | TGGCCA | 1 cut(s) | 102 |
| MseI | TTAA | 2 cut(s) | 147, 744 |
| MslI | CAYNNNNRTG | 2 cut(s) | 594, 1221 |
| Msp20I | TGGCCA | 1 cut(s) | 102 |
| MspI | CCGG | 3 cut(s) | 36, 367, 1047 |
| MspR9I | CCNGG | 4 cut(s) | 44, 111, 584, 1092 |
| Mva1269I | GAATGC | 1 cut(s) | 991 |
| MvaI | CCWGG | 4 cut(s) | 44, 111, 584, 1092 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 57, 360, 839, 995, 1205, 1316 |
| NdeI | CATATG | 1 cut(s) | 814 |
| NdeII | GATC | 5 cut(s) | 205, 346, 1131, 1186, 1270 |
| NlaIII | CATG | 8 cut(s) | 86, 185, 851, 904, 929, 940, 1132, 1195 |
| NlaIV | GGNNCC | 5 cut(s) | 244, 739, 780, 1040, 1089 |
| NmuCI | GTSAC | 4 cut(s) | 113, 317, 754, 914 |
| NspI | RCATGY | 1 cut(s) | 185 |
| PaeR7I | CTCGAG | 1 cut(s) | 209 |
| PciSI | GCTCTTC | 1 cut(s) | 263 |
| PctI | GAATGC | 1 cut(s) | 991 |
| PfeI | GAWTC | 2 cut(s) | 792, 1050 |
| PfoI | TCCNGGA | 2 cut(s) | 582, 1090 |
| PkrI | GCNGC | 4 cut(s) | 284, 298, 991, 1279 |
| PleI | GAGTC | 1 cut(s) | 1321 |
| PpsI | GAGTC | 1 cut(s) | 1321 |
| PshAI | GACNNNNGTC | 2 cut(s) | 921, 1148 |
| Psp6I | CCWGG | 4 cut(s) | 42, 109, 582, 1090 |
| PspFI | CCCAGC | 1 cut(s) | 60 |
| PspGI | CCWGG | 4 cut(s) | 42, 109, 582, 1090 |
| PspN4I | GGNNCC | 5 cut(s) | 244, 739, 780, 1040, 1089 |
| PspPI | GGNCC | 3 cut(s) | 19, 358, 1111 |
| PstI | CTGCAG | 1 cut(s) | 641 |
| PstNI | CAGNNNCTG | 2 cut(s) | 866, 1316 |
| PsuI | RGATCY | 1 cut(s) | 1270 |
| RsaI | GTAC | 4 cut(s) | 244, 845, 956, 1349 |
| RsaNI | GTAC | 4 cut(s) | 243, 844, 955, 1348 |
| RseI | CAYNNNNRTG | 2 cut(s) | 594, 1221 |
| SapI | GCTCTTC | 1 cut(s) | 263 |
| SaqAI | TTAA | 2 cut(s) | 147, 744 |
| SatI | GCNGC | 4 cut(s) | 283, 297, 990, 1278 |
| Sau3AI | GATC | 5 cut(s) | 205, 346, 1131, 1186, 1270 |
| Sau96I | GGNCC | 3 cut(s) | 19, 358, 1111 |
| ScaI | AGTACT | 1 cut(s) | 1349 |
| SchI | GAGTC | 1 cut(s) | 1322 |
| ScrFI | CCNGG | 4 cut(s) | 44, 111, 584, 1092 |
| SduI | GDGCHC | 8 cut(s) | 216, 552, 609, 742, 894, 975, 1043, 1218 |
| SfaNI | GCATC | 3 cut(s) | 130, 283, 372 |
| SfcI | CTRYAG | 1 cut(s) | 637 |
| Sfr274I | CTCGAG | 1 cut(s) | 209 |
| SinI | GGWCC | 3 cut(s) | 19, 358, 1111 |
| SlaI | CTCGAG | 1 cut(s) | 209 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 594, 1221 |
| SmlI | CTYRAG | 3 cut(s) | 77, 209, 1283 |
| SmoI | CTYRAG | 3 cut(s) | 77, 209, 1283 |
| Sse9I | AATT | 2 cut(s) | 761, 1170 |
| SsiI | CCGC | 5 cut(s) | 250, 282, 361, 833, 1059 |
| StyD4I | CCNGG | 4 cut(s) | 42, 109, 582, 1090 |
| StyI | CCWWGG | 2 cut(s) | 975, 1107 |
| TaaI | ACNGT | 8 cut(s) | 124, 220, 511, 560, 686, 881, 959, 1084 |
| TaiI | ACGT | 1 cut(s) | 469 |
| TaqI | TCGA | 3 cut(s) | 210, 732, 1142 |
| TaqII | GACCGA | 2 cut(s) | 7, 17 |
| TasI | AATT | 2 cut(s) | 761, 1170 |
| TatI | WGTACW | 1 cut(s) | 1347 |
| TauI | GCSGC | 1 cut(s) | 285 |
| TfiI | GAWTC | 2 cut(s) | 792, 1050 |
| Tru1I | TTAA | 2 cut(s) | 147, 744 |
| Tru9I | TTAA | 2 cut(s) | 147, 744 |
| TseFI | GTSAC | 4 cut(s) | 113, 317, 754, 914 |
| TseI | GCWGC | 3 cut(s) | 296, 989, 1277 |
| Tsp45I | GTSAC | 4 cut(s) | 113, 317, 754, 914 |
| TspDTI | ATGAA | 2 cut(s) | 243, 1311 |
| VneI | GTGCAC | 1 cut(s) | 1214 |
| VpaK11BI | GGWCC | 3 cut(s) | 19, 358, 1111 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1106 |
| XapI | RAATTY | 1 cut(s) | 761 |
| XceI | RCATGY | 1 cut(s) | 185 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1027 |
| XhoI | CTCGAG | 1 cut(s) | 209 |
| ZrmI | AGTACT | 1 cut(s) | 1349 |