Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 122 |
| Acc65I | GGTACC | 1 cut(s) | 431 |
| AccB1I | GGYRCC | 4 cut(s) | 170, 242, 431, 1038 |
| AccBSI | CCGCTC | 1 cut(s) | 466 |
| AccII | CGCG | 3 cut(s) | 132, 192, 1206 |
| AclWI | GGATC | 6 cut(s) | 13, 445, 787, 800, 911, 1278 |
| AcoI | YGGCCR | 4 cut(s) | 100, 127, 906, 996 |
| AcyI | GRCGYC | 2 cut(s) | 141, 294 |
| AfaI | GTAC | 6 cut(s) | 433, 845, 935, 956, 1196, 1223 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 726, 926, 1108 |
| AgeI | ACCGGT | 1 cut(s) | 167 |
| AgsI | TTSAA | 3 cut(s) | 148, 415, 1054 |
| AjiI | CACGTC | 2 cut(s) | 184, 982 |
| AjnI | CCWGG | 3 cut(s) | 525, 788, 1106 |
| AjuI | GAANNNNNNNTTGG | 6 cut(s) | 488, 520, 869, 901, 1193, 1225 |
| AleI | CACNNNNGTG | 1 cut(s) | 596 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1235, 1267 |
| AluBI | AGCT | 7 cut(s) | 67, 256, 274, 373, 499, 740, 856 |
| AluI | AGCT | 7 cut(s) | 67, 256, 274, 373, 499, 740, 856 |
| Alw21I | GWGCWC | 4 cut(s) | 69, 84, 591, 742 |
| Alw26I | GTCTC | 4 cut(s) | 158, 449, 697, 1338 |
| AlwI | GGATC | 6 cut(s) | 13, 445, 787, 800, 911, 1278 |
| Ama87I | CYCGRG | 2 cut(s) | 77, 446 |
| AoxI | GGCC | 6 cut(s) | 91, 100, 127, 906, 939, 996 |
| ApeKI | GCWGC | 4 cut(s) | 271, 853, 945, 1277 |
| AsiGI | ACCGGT | 1 cut(s) | 167 |
| Asp718I | GGTACC | 1 cut(s) | 431 |
| AspLEI | GCGC | 4 cut(s) | 194, 1206, 1208, 1310 |
| AspS9I | GGNCC | 3 cut(s) | 92, 232, 645 |
| AsuC2I | CCSGG | 2 cut(s) | 391, 1092 |
| AsuHPI | GGTGA | 5 cut(s) | 865, 974, 1109, 1241, 1346 |
| AvaI | CYCGRG | 2 cut(s) | 77, 446 |
| AvaII | GGWCC | 2 cut(s) | 232, 645 |
| BaeGI | GKGCMC | 1 cut(s) | 1043 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1213, 1246 |
| BalI | TGGCCA | 3 cut(s) | 102, 908, 998 |
| BamHI | GGATCC | 1 cut(s) | 792 |
| BanI | GGYRCC | 4 cut(s) | 170, 242, 431, 1038 |
| BanII | GRGCYC | 4 cut(s) | 69, 216, 396, 742 |
| BauI | CACGAG | 1 cut(s) | 1263 |
| BbsI | GAAGAC | 2 cut(s) | 297, 403 |
| Bbv12I | GWGCWC | 4 cut(s) | 69, 84, 591, 742 |
| BbvCI | CCTCAGC | 2 cut(s) | 725, 1155 |
| BbvI | GCAGC | 4 cut(s) | 283, 865, 932, 1264 |
| BccI | CCATC | 7 cut(s) | 92, 203, 335, 632, 898, 1010, 1325 |
| BceAI | ACGGC | 3 cut(s) | 100, 404, 624 |
| BcgI | CGANNNNNNTGC | 6 cut(s) | 152, 170, 186, 204, 322, 356 |
| BciT130I | CCWGG | 3 cut(s) | 527, 790, 1108 |
| BciVI | GTATCC | 1 cut(s) | 525 |
| BclI | TGATCA | 1 cut(s) | 1174 |
| BcnI | CCSGG | 2 cut(s) | 391, 1092 |
| BcoDI | GTCTC | 4 cut(s) | 158, 449, 697, 1338 |
| BfaI | CTAG | 1 cut(s) | 500 |
| BfoI | RGCGCY | 1 cut(s) | 1311 |
| BfuI | GTATCC | 1 cut(s) | 525 |
| BglI | GCCNNNNNGGC | 3 cut(s) | 99, 839, 995 |
| BglII | AGATCT | 1 cut(s) | 346 |
| BisI | GCNGC | 9 cut(s) | 130, 133, 272, 297, 467, 854, 946, 990, 1278 |
| BlsI | GCNGC | 9 cut(s) | 131, 134, 273, 298, 468, 855, 947, 991, 1279 |
| Bme1390I | CCNGG | 5 cut(s) | 391, 527, 790, 1092, 1108 |
| Bme18I | GGWCC | 2 cut(s) | 232, 645 |
| BmeT110I | CYCGRG | 2 cut(s) | 77, 446 |
| BmgBI | CACGTC | 2 cut(s) | 184, 982 |
| BmgT120I | GGNCC | 3 cut(s) | 92, 232, 645 |
| BmrFI | CCNGG | 5 cut(s) | 391, 527, 790, 1092, 1108 |
| BmrI | ACTGGG | 1 cut(s) | 1056 |
| BmsI | GCATC | 1 cut(s) | 96 |
| BmuI | ACTGGG | 1 cut(s) | 1056 |
| BpiI | GAAGAC | 2 cut(s) | 297, 403 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1148, 1180 |
| BpmI | CTGGAG | 4 cut(s) | 230, 482, 605, 1304 |
| Bpu10I | CCTNAGC | 3 cut(s) | 267, 725, 1155 |
| BpuMI | CCSGG | 2 cut(s) | 391, 1092 |
| BsaAI | YACGTR | 1 cut(s) | 1225 |
| BsaBI | GATNNNNATC | 1 cut(s) | 23 |
| BsaHI | GRCGYC | 2 cut(s) | 141, 294 |
| BsaI | GGTCTC | 2 cut(s) | 158, 449 |
| BsaWI | WCCGGW | 3 cut(s) | 167, 434, 820 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 726, 926, 1108 |
| Bse118I | RCCGGY | 3 cut(s) | 167, 173, 239 |
| Bse1I | ACTGG | 3 cut(s) | 1024, 1051, 1146 |
| Bse8I | GATNNNNATC | 1 cut(s) | 23 |
| BseBI | CCWGG | 3 cut(s) | 527, 790, 1108 |
| BseGI | GGATG | 1 cut(s) | 757 |
| BseJI | GATNNNNATC | 1 cut(s) | 23 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 726, 926, 1108 |
| BseMII | CTCAG | 7 cut(s) | 258, 710, 716, 750, 858, 1146, 1302 |
| BseNI | ACTGG | 3 cut(s) | 1024, 1051, 1146 |
| BsePI | GCGCGC | 1 cut(s) | 1204 |
| BseRI | GAGGAG | 1 cut(s) | 1313 |
| BseSI | GKGCMC | 1 cut(s) | 1043 |
| BseX3I | CGGCCG | 1 cut(s) | 127 |
| BseXI | GCAGC | 4 cut(s) | 283, 865, 932, 1264 |
| BseYI | CCCAGC | 2 cut(s) | 60, 110 |
| BsgI | GTGCAG | 2 cut(s) | 370, 1133 |
| Bsh1236I | CGCG | 3 cut(s) | 132, 192, 1206 |
| Bsh1285I | CGRYCG | 2 cut(s) | 130, 361 |
| BshFI | GGCC | 6 cut(s) | 93, 102, 129, 908, 941, 998 |
| BshNI | GGYRCC | 4 cut(s) | 170, 242, 431, 1038 |
| BshTI | ACCGGT | 1 cut(s) | 167 |
| BsiEI | CGRYCG | 2 cut(s) | 130, 361 |
| BsiHKAI | GWGCWC | 4 cut(s) | 69, 84, 591, 742 |
| BsiHKCI | CYCGRG | 2 cut(s) | 77, 446 |
| BsiSI | CCGG | 9 cut(s) | 36, 168, 174, 240, 282, 390, 435, 821, 1091 |
| BslFI | GGGAC | 3 cut(s) | 170, 631, 648 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 726, 926, 1108 |
| BsmAI | GTCTC | 4 cut(s) | 158, 449, 697, 1338 |
| BsmBI | CGTCTC | 2 cut(s) | 697, 1338 |
| BsmFI | GGGAC | 3 cut(s) | 170, 631, 648 |
| BsmI | GAATGC | 2 cut(s) | 884, 991 |
| BsnI | GGCC | 6 cut(s) | 93, 102, 129, 908, 941, 998 |
| Bso31I | GGTCTC | 2 cut(s) | 158, 449 |
| BsoBI | CYCGRG | 2 cut(s) | 77, 446 |
| Bsp1286I | GDGCHC | 7 cut(s) | 69, 84, 216, 396, 591, 742, 1043 |
| Bsp19I | CCATGG | 1 cut(s) | 925 |
| BspANI | GGCC | 6 cut(s) | 93, 102, 129, 908, 941, 998 |
| BspCNI | CTCAG | 7 cut(s) | 259, 709, 717, 751, 859, 1147, 1303 |
| BspFNI | CGCG | 3 cut(s) | 132, 192, 1206 |
| BspPI | GGATC | 6 cut(s) | 13, 445, 787, 800, 911, 1278 |
| BspQI | GCTCTTC | 1 cut(s) | 263 |
| BspT107I | GGYRCC | 4 cut(s) | 170, 242, 431, 1038 |
| BspTNI | GGTCTC | 2 cut(s) | 158, 449 |
| BsrBI | CCGCTC | 1 cut(s) | 466 |
| BsrFI | RCCGGY | 3 cut(s) | 167, 173, 239 |
| BsrI | ACTGG | 3 cut(s) | 1024, 1051, 1146 |
| BssAI | RCCGGY | 3 cut(s) | 167, 173, 239 |
| BssHII | GCGCGC | 1 cut(s) | 1204 |
| BssNI | GRCGYC | 2 cut(s) | 141, 294 |
| BssSI | CACGAG | 1 cut(s) | 1263 |
| BssT1I | CCWWGG | 3 cut(s) | 397, 925, 975 |
| Bst2BI | CACGAG | 1 cut(s) | 1263 |
| Bst2UI | CCWGG | 3 cut(s) | 527, 790, 1108 |
| Bst4CI | ACNGT | 6 cut(s) | 124, 220, 511, 560, 881, 1084 |
| Bst6I | CTCTTC | 1 cut(s) | 263 |
| BstACI | GRCGYC | 2 cut(s) | 141, 294 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 57 |
| BstBAI | YACGTR | 1 cut(s) | 1225 |
| BstC8I | GCNNGC | 9 cut(s) | 33, 58, 175, 179, 254, 375, 894, 943, 1206 |
| BstDEI | CTNAG | 7 cut(s) | 267, 696, 725, 759, 867, 1155, 1311 |
| BstDSI | CCRYGG | 3 cut(s) | 130, 313, 925 |
| BstEII | GGTNACC | 1 cut(s) | 1097 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 724 |
| BstF5I | GGATG | 1 cut(s) | 757 |
| BstFNI | CGCG | 3 cut(s) | 132, 192, 1206 |
| BstH2I | RGCGCY | 1 cut(s) | 1311 |
| BstHHI | GCGC | 4 cut(s) | 194, 1206, 1208, 1310 |
| BstMAI | GTCTC | 4 cut(s) | 158, 449, 697, 1338 |
| BstMCI | CGRYCG | 2 cut(s) | 130, 361 |
| BstNI | CCWGG | 3 cut(s) | 527, 790, 1108 |
| BstPI | GGTNACC | 1 cut(s) | 1097 |
| BstSCI | CCNGG | 5 cut(s) | 389, 525, 788, 1090, 1106 |
| BstSLI | GKGCMC | 1 cut(s) | 1043 |
| BstUI | CGCG | 3 cut(s) | 132, 192, 1206 |
| BstV1I | GCAGC | 4 cut(s) | 283, 865, 932, 1264 |
| BstV2I | GAAGAC | 2 cut(s) | 297, 403 |
| BstX2I | RGATCY | 3 cut(s) | 346, 792, 1270 |
| BstYI | RGATCY | 3 cut(s) | 346, 792, 1270 |
| BstZI | CGGCCG | 1 cut(s) | 127 |
| BsuI | GTATCC | 1 cut(s) | 525 |
| BsuRI | GGCC | 6 cut(s) | 93, 102, 129, 908, 941, 998 |
| BtgI | CCRYGG | 3 cut(s) | 130, 313, 925 |
| BtrI | CACGTC | 2 cut(s) | 184, 982 |
| BtsCI | GGATG | 1 cut(s) | 757 |
| BtsIMutI | CAGTG | 4 cut(s) | 507, 565, 886, 1153 |
| Cac8I | GCNNGC | 9 cut(s) | 33, 58, 175, 179, 254, 375, 894, 943, 1206 |
| CfoI | GCGC | 4 cut(s) | 194, 1206, 1208, 1310 |
| Cfr10I | RCCGGY | 3 cut(s) | 167, 173, 239 |
| Cfr13I | GGNCC | 3 cut(s) | 92, 232, 645 |
| Cfr42I | CCGCGG | 1 cut(s) | 133 |
| CseI | GACGC | 3 cut(s) | 149, 302, 650 |
| Csp6I | GTAC | 6 cut(s) | 432, 844, 934, 955, 1195, 1222 |
| CspAI | ACCGGT | 1 cut(s) | 167 |
| CviAII | CATG | 7 cut(s) | 802, 848, 901, 926, 937, 1129, 1237 |
| CviQI | GTAC | 6 cut(s) | 432, 844, 934, 955, 1195, 1222 |
| DdeI | CTNAG | 7 cut(s) | 267, 696, 725, 759, 867, 1155, 1311 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 122 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 122 |
| EaeI | YGGCCR | 4 cut(s) | 100, 127, 906, 996 |
| EagI | CGGCCG | 1 cut(s) | 127 |
| Eam1104I | CTCTTC | 1 cut(s) | 263 |
| EarI | CTCTTC | 1 cut(s) | 263 |
| EciI | GGCGGA | 1 cut(s) | 820 |
| Ecl136II | GAGCTC | 2 cut(s) | 67, 740 |
| EclXI | CGGCCG | 1 cut(s) | 127 |
| Eco130I | CCWWGG | 3 cut(s) | 397, 925, 975 |
| Eco24I | GRGCYC | 4 cut(s) | 69, 216, 396, 742 |
| Eco31I | GGTCTC | 2 cut(s) | 158, 449 |
| Eco32I | GATATC | 1 cut(s) | 655 |
| Eco47I | GGWCC | 2 cut(s) | 232, 645 |
| Eco52I | CGGCCG | 1 cut(s) | 127 |
| Eco53kI | GAGCTC | 2 cut(s) | 67, 740 |
| Eco88I | CYCGRG | 2 cut(s) | 77, 446 |
| Eco91I | GGTNACC | 1 cut(s) | 1097 |
| EcoICRI | GAGCTC | 2 cut(s) | 67, 740 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 724 |
| EcoO65I | GGTNACC | 1 cut(s) | 1097 |
| EcoRII | CCWGG | 3 cut(s) | 525, 788, 1106 |
| EcoRV | GATATC | 1 cut(s) | 655 |
| EcoT14I | CCWWGG | 3 cut(s) | 397, 925, 975 |
| EcoT38I | GRGCYC | 4 cut(s) | 69, 216, 396, 742 |
| ErhI | CCWWGG | 3 cut(s) | 397, 925, 975 |
| Esp3I | CGTCTC | 2 cut(s) | 697, 1338 |
| FaeI | CATG | 7 cut(s) | 805, 851, 904, 929, 940, 1132, 1240 |
| FaiI | YATR | 9 cut(s) | 784, 803, 816, 849, 902, 927, 938, 1130, 1238 |
| FaqI | GGGAC | 3 cut(s) | 170, 631, 648 |
| FatI | CATG | 7 cut(s) | 801, 847, 900, 925, 936, 1128, 1236 |
| FauI | CCCGC | 1 cut(s) | 929 |
| FbaI | TGATCA | 1 cut(s) | 1174 |
| Fnu4HI | GCNGC | 9 cut(s) | 130, 133, 272, 297, 467, 854, 946, 990, 1278 |
| FokI | GGATG | 1 cut(s) | 764 |
| FriOI | GRGCYC | 4 cut(s) | 69, 216, 396, 742 |
| Fsp4HI | GCNGC | 9 cut(s) | 130, 133, 272, 297, 467, 854, 946, 990, 1278 |
| FspBI | CTAG | 1 cut(s) | 500 |
| GlaI | GCGC | 4 cut(s) | 193, 1205, 1207, 1309 |
| GluI | GCNGC | 9 cut(s) | 130, 133, 272, 297, 467, 854, 946, 990, 1278 |
| GsaI | CCCAGC | 2 cut(s) | 64, 114 |
| GsuI | CTGGAG | 4 cut(s) | 230, 482, 605, 1304 |
| HaeII | RGCGCY | 1 cut(s) | 1311 |
| HaeIII | GGCC | 6 cut(s) | 93, 102, 129, 908, 941, 998 |
| HapII | CCGG | 9 cut(s) | 36, 168, 174, 240, 282, 390, 435, 821, 1091 |
| HgaI | GACGC | 3 cut(s) | 149, 302, 650 |
| HhaI | GCGC | 4 cut(s) | 194, 1206, 1208, 1310 |
| Hin1I | GRCGYC | 2 cut(s) | 141, 294 |
| Hin1II | CATG | 7 cut(s) | 805, 851, 904, 929, 940, 1132, 1240 |
| Hin6I | GCGC | 4 cut(s) | 192, 1204, 1206, 1308 |
| HinP1I | GCGC | 4 cut(s) | 192, 1204, 1206, 1308 |
| HinfI | GANTC | 4 cut(s) | 39, 504, 682, 1313 |
| HpaII | CCGG | 9 cut(s) | 36, 168, 174, 240, 282, 390, 435, 821, 1091 |
| HphI | GGTGA | 5 cut(s) | 865, 974, 1109, 1241, 1346 |
| Hpy188I | TCNGA | 5 cut(s) | 163, 681, 744, 1191, 1258 |
| Hpy188III | TCNNGA | 6 cut(s) | 209, 461, 766, 1006, 1265, 1283 |
| Hpy99I | CGWCG | 8 cut(s) | 128, 140, 143, 206, 230, 425, 645, 737 |
| HpyAV | CCTTC | 5 cut(s) | 154, 163, 528, 832, 1238 |
| HpyCH4III | ACNGT | 6 cut(s) | 124, 220, 511, 560, 881, 1084 |
| HpyCH4IV | ACGT | 3 cut(s) | 183, 981, 1224 |
| HpyCH4V | TGCA | 6 cut(s) | 12, 387, 884, 1114, 1128, 1216 |
| HpyF3I | CTNAG | 7 cut(s) | 267, 696, 725, 759, 867, 1155, 1311 |
| HpySE526I | ACGT | 3 cut(s) | 183, 981, 1224 |
| Hsp92I | GRCGYC | 2 cut(s) | 141, 294 |
| Hsp92II | CATG | 7 cut(s) | 805, 851, 904, 929, 940, 1132, 1240 |
| HspAI | GCGC | 4 cut(s) | 192, 1204, 1206, 1308 |
| KpnI | GGTACC | 1 cut(s) | 435 |
| KroI | GCCGGC | 1 cut(s) | 173 |
| KroNI | GCCGGC | 1 cut(s) | 175 |
| Ksp22I | TGATCA | 1 cut(s) | 1174 |
| KspI | CCGCGG | 1 cut(s) | 133 |
| LguI | GCTCTTC | 1 cut(s) | 263 |
| LmnI | GCTCC | 8 cut(s) | 64, 211, 399, 463, 547, 586, 737, 823 |
| Lsp1109I | GCAGC | 4 cut(s) | 283, 865, 932, 1264 |
| LweI | GCATC | 1 cut(s) | 96 |
| MaeI | CTAG | 1 cut(s) | 500 |
| MaeII | ACGT | 3 cut(s) | 183, 981, 1224 |
| MaeIII | GTNAC | 3 cut(s) | 317, 754, 1097 |
| MbiI | CCGCTC | 1 cut(s) | 466 |
| MboII | GAAGA | 6 cut(s) | 148, 190, 250, 302, 403, 699 |
| MflI | RGATCY | 3 cut(s) | 346, 792, 1270 |
| MhlI | GDGCHC | 7 cut(s) | 69, 84, 216, 396, 591, 742, 1043 |
| MlsI | TGGCCA | 3 cut(s) | 102, 908, 998 |
| MluCI | AATT | 1 cut(s) | 1066 |
| MluNI | TGGCCA | 3 cut(s) | 102, 908, 998 |
| MlyI | GAGTC | 1 cut(s) | 1322 |
| MmeI | TCCRAC | 1 cut(s) | 1161 |
| Mox20I | TGGCCA | 3 cut(s) | 102, 908, 998 |
| MroNI | GCCGGC | 1 cut(s) | 173 |
| MscI | TGGCCA | 3 cut(s) | 102, 908, 998 |
| MslI | CAYNNNNRTG | 2 cut(s) | 594, 596 |
| Msp20I | TGGCCA | 3 cut(s) | 102, 908, 998 |
| MspA1I | CMGCKG | 2 cut(s) | 132, 992 |
| MspI | CCGG | 9 cut(s) | 36, 168, 174, 240, 282, 390, 435, 821, 1091 |
| MspR9I | CCNGG | 5 cut(s) | 391, 527, 790, 1092, 1108 |
| Mva1269I | GAATGC | 2 cut(s) | 884, 991 |
| MvaI | CCWGG | 3 cut(s) | 527, 790, 1108 |
| MvnI | CGCG | 3 cut(s) | 132, 192, 1206 |
| NaeI | GCCGGC | 1 cut(s) | 175 |
| NciI | CCSGG | 2 cut(s) | 391, 1092 |
| NcoI | CCATGG | 1 cut(s) | 925 |
| NgoMIV | GCCGGC | 1 cut(s) | 173 |
| NlaIII | CATG | 7 cut(s) | 805, 851, 904, 929, 940, 1132, 1240 |
| NmeAIII | GCCGAG | 2 cut(s) | 859, 1345 |
| NmuCI | GTSAC | 3 cut(s) | 317, 754, 1097 |
| OliI | CACNNNNGTG | 1 cut(s) | 596 |
| PaeR7I | CTCGAG | 1 cut(s) | 77 |
| PauI | GCGCGC | 1 cut(s) | 1204 |
| PciSI | GCTCTTC | 1 cut(s) | 263 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 132 |
| PctI | GAATGC | 2 cut(s) | 884, 991 |
| PdiI | GCCGGC | 1 cut(s) | 175 |
| PfeI | GAWTC | 3 cut(s) | 39, 504, 682 |
| PflFI | GACNNNGTC | 1 cut(s) | 230 |
| PinAI | ACCGGT | 1 cut(s) | 167 |
| PkrI | GCNGC | 9 cut(s) | 131, 134, 273, 298, 468, 855, 947, 991, 1279 |
| PleI | GAGTC | 1 cut(s) | 1321 |
| PpsI | GAGTC | 1 cut(s) | 1321 |
| Ppu21I | YACGTR | 1 cut(s) | 1225 |
| Psp124BI | GAGCTC | 2 cut(s) | 69, 742 |
| Psp6I | CCWGG | 3 cut(s) | 525, 788, 1106 |
| PspEI | GGTNACC | 1 cut(s) | 1097 |
| PspFI | CCCAGC | 2 cut(s) | 60, 110 |
| PspGI | CCWGG | 3 cut(s) | 525, 788, 1106 |
| PspPI | GGNCC | 3 cut(s) | 92, 232, 645 |
| PspXI | VCTCGAGB | 1 cut(s) | 77 |
| PsuI | RGATCY | 3 cut(s) | 346, 792, 1270 |
| PsyI | GACNNNGTC | 1 cut(s) | 230 |
| PteI | GCGCGC | 1 cut(s) | 1204 |
| RsaI | GTAC | 6 cut(s) | 433, 845, 935, 956, 1196, 1223 |
| RsaNI | GTAC | 6 cut(s) | 432, 844, 934, 955, 1195, 1222 |
| RseI | CAYNNNNRTG | 2 cut(s) | 594, 596 |
| SacI | GAGCTC | 2 cut(s) | 69, 742 |
| SacII | CCGCGG | 1 cut(s) | 133 |
| SapI | GCTCTTC | 1 cut(s) | 263 |
| SatI | GCNGC | 9 cut(s) | 130, 133, 272, 297, 467, 854, 946, 990, 1278 |
| Sau96I | GGNCC | 3 cut(s) | 92, 232, 645 |
| SchI | GAGTC | 1 cut(s) | 1322 |
| ScrFI | CCNGG | 5 cut(s) | 391, 527, 790, 1092, 1108 |
| SduI | GDGCHC | 7 cut(s) | 69, 84, 216, 396, 591, 742, 1043 |
| SfaNI | GCATC | 1 cut(s) | 96 |
| SfiI | GGCCNNNNNGGCC | 1 cut(s) | 99 |
| Sfr274I | CTCGAG | 1 cut(s) | 77 |
| Sfr303I | CCGCGG | 1 cut(s) | 133 |
| SgrBI | CCGCGG | 1 cut(s) | 133 |
| SinI | GGWCC | 2 cut(s) | 232, 645 |
| SlaI | CTCGAG | 1 cut(s) | 77 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 594, 596 |
| SmlI | CTYRAG | 1 cut(s) | 77 |
| SmoI | CTYRAG | 1 cut(s) | 77 |
| Sse9I | AATT | 1 cut(s) | 1066 |
| SspMI | CTAG | 1 cut(s) | 500 |
| SstI | GAGCTC | 2 cut(s) | 69, 742 |
| StyD4I | CCNGG | 5 cut(s) | 389, 525, 788, 1090, 1106 |
| StyI | CCWWGG | 3 cut(s) | 397, 925, 975 |
| TaaI | ACNGT | 6 cut(s) | 124, 220, 511, 560, 881, 1084 |
| TaiI | ACGT | 3 cut(s) | 186, 984, 1227 |
| TaqII | GACCGA | 1 cut(s) | 633 |
| TasI | AATT | 1 cut(s) | 1066 |
| TatI | WGTACW | 1 cut(s) | 933 |
| TauI | GCSGC | 5 cut(s) | 132, 135, 299, 469, 992 |
| TfiI | GAWTC | 3 cut(s) | 39, 504, 682 |
| TscAI | CASTG | 4 cut(s) | 514, 565, 886, 1153 |
| TseFI | GTSAC | 3 cut(s) | 317, 754, 1097 |
| TseI | GCWGC | 4 cut(s) | 271, 853, 945, 1277 |
| Tsp45I | GTSAC | 3 cut(s) | 317, 754, 1097 |
| TspDTI | ATGAA | 2 cut(s) | 633, 790 |
| TspGWI | ACGGA | 2 cut(s) | 498, 750 |
| TspRI | CASTG | 4 cut(s) | 514, 565, 886, 1153 |
| Tth111I | GACNNNGTC | 1 cut(s) | 230 |
| VpaK11BI | GGWCC | 2 cut(s) | 232, 645 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 724 |
| XhoI | CTCGAG | 1 cut(s) | 77 |
| XspI | CTAG | 1 cut(s) | 500 |