Rmu_sc0003113.1_g000016

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003113.1
OG0000549
Physical Location & Seq
Reverse (-)
78747 .. 80706
1960 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003113.1_g000016.1.cds

Sequence Viewer

Length: 1356 bp
atgagagagtgcatttcgatccacatcggccaggccggaatccaggtcggaaacgcctgctgggagctttactgtctcgagcatgggatccagccggacggccagatgccgagcgacaagaccgtcggccgcggcgacgacgccttcaacaccttcttctccgagaccggcgccggcaagcacgtcccgcgcgccatcttcgtcgacctcgagcccaccgtcatcgacgaggtccgcaccggaacctaccgccagctcttccaccccgagcagctcatctccggcaaggaggacgccgccaacaacttcgctcgcggccattacaccatcgggaaagagatcgtcgatctatgcctggatcggatccggaagctcgctgataactgcaccgggctccagggattcctggtcttcaatgccgtcggtggcggtaccgggtcgggtctcgggtcgcttctgctggagaggctttctgtggactatgggaaaaagtccaagctcggattcactgtttatccttctcctcaggtttccacatctgttgtggagccatataacagtgtgctctccactcattcgctgcttgagcacaccgatgtgtctgttttgctcgataatgaggccatttacgacatctgccgccgatccctggacattgagcgtcccacctacaccaatctcaatcgcctcgtctctcaggtgatttcatcattgacagcctccctgaggtttgatggggctctgaatgtggatgtgactgagttccagaccaacttggttccatacccaaggatccatttcatgctctcctcctatgctcctgtgatctccgcagagaaagcataccacgagcagctctcagtggctgagatcaccaacagtgcttttgagccagcttccatgatggccaagtgtgatcctcgccatggcaagtacatggcctgctgtttgatgtaccgaggagatgtggtgcctaaggatgtgaatgcggctgtggccaccatcaagaccaagcgtaccattcagtttgtggactggtgccctactgggttcaagtgtggtatcaactaccagccacccactgttgttccaggcggtgaccttgctaaggtgcagagggctgtgtgcatgatctccaactccaccagtgtggctgaggtgttctcccgcattgatcacaagtttgatctgatgtatgccaagcgtgcttttgtgcattggtatgtgggtgagggcatggaggaaggagagttctctgaggctcgtgaggatctcgctgctctggagaaggattatgaggaggttggtgccgagtctgctgagggagatgatggcgatgaaggagacgagtactga

Protein Analysis

451

Amino Acids

49.73

Weight (kDa)

4.96

Isoelectric Point (pI)

35.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000549)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04820 AT1G50010 AT4G14960 AT4G14960
fragaria_vesca FvH4_1g18660 FvH4_2g03140 FvH4_5g33560 FvH4_6g48940 FvH4_6g48940
malus_domestica MD02G1204800.v1.1 MD05G1106800.v1.1 MD08G1210600.v1.1 MD09G1049500.v1.1 MD10G1111600.v1.1 MD15G1398700.v1.1 MD17G1049700.v1.1
prunus_persica Prupe.1G542600_v2.0.a1 Prupe.3G269300_v2.0.a1 Prupe.6G212700_v2.0.a1 Prupe.8G150900_v2.0.a1
pyrus_communis pycom05g10370 pycom08g18150 pycom10g09640 pycom10g09660 pycom111g04050 pycom15g35470 pycom17g04460
rosa_chinensis RchiOBHm_Chr2g0109481 RchiOBHm_Chr2g0168751 RchiOBHm_Chr3g0453071 RchiOBHm_Chr3g0460741 RchiOBHm_Chr3g0467971 RchiOBHm_Chr4g0400901 RchiOBHm_Chr6g0248011 RchiOBHm_Chr7g0232381 RchiOBHm_Chr7g0238121
rosa_laevigata RLG00000001350 RLG00000009167 RLG00000015163 RLG00000017708 RLG00000017741 RLG00000021807 RLG00000023849 RLG00000026518
rosa_multiflora Rmu_sc0000332.1_g000021 Rmu_sc0000332.1_g000029 Rmu_sc0002416.1_g000007 Rmu_sc0002611.1_g000001 Rmu_sc0003113.1_g000016 Rmu_sc0004780.1_g000001 Rmu_sc0004805.1_g000035 Rmu_sc0010633.1_g000010 Rmu_sc0011069.1_g000003 Rmu_sc0016170.1_g000002 Rmu_sc0025757.1_g000003 Rmu_ssc0000484.1_g000003
rosa_roxburghii Rroxscaffold_2G00134200 Rroxscaffold_4G00321030 Rroxscaffold_7G00213500
rosa_rugosa Rorug01G0283100 Rorug02G0160500 Rorug02G0535300 Rorug04G0027000 Rorug04G0328100 Rorug05G0388700 Rorug05G0530200 Rorug06G0489200 Rorug07G0104900 Rorug07G0209400 Rorug07G0274500
rosa_samantha Rh2BG222800 Rh2BG618900 Rh2CG214400 Rh2CG587200 Rh6BG040200 Rh6CG206500 Rh6DG035600 Rh7BG401600 Rh7BG401700 Rh7DG418400
rosa_wichuraiana Rw2G050240 Rw6G003920 Rw7G035430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 122
Acc65I GGTACC 1 cut(s) 431
AccB1I GGYRCC 5 cut(s) 170, 431, 970, 1038, 1307
AccI GTMKAC 1 cut(s) 204
AccII CGCG 4 cut(s) 132, 190, 192, 315
AccIII TCCGGA 1 cut(s) 366
AcoI YGGCCR 6 cut(s) 28, 100, 127, 316, 906, 996
AcyI GRCGYC 3 cut(s) 141, 171, 294
AfaI GTAC 5 cut(s) 433, 935, 956, 1018, 1352
AfiI CCNNNNNNNGG 4 cut(s) 649, 726, 926, 1108
AgsI TTSAA 3 cut(s) 148, 415, 1054
AjiI CACGTC 1 cut(s) 184
AjnI CCWGG 7 cut(s) 30, 42, 354, 396, 405, 648, 1090
AjuI GAANNNNNNNTTGG 2 cut(s) 488, 520
AleI CACNNNNGTG 2 cut(s) 596, 1148
AloI GAACNNNNNNTCC 2 cut(s) 1235, 1267
AluBI AGCT 7 cut(s) 67, 256, 274, 373, 499, 856, 896
AluI AGCT 7 cut(s) 67, 256, 274, 373, 499, 856, 896
Alw21I GWGCWC 2 cut(s) 567, 591
Alw26I GTCTC 5 cut(s) 80, 158, 449, 697, 1338
AlwNI CAGNNNCTG 1 cut(s) 866
Ama87I CYCGRG 4 cut(s) 77, 209, 266, 446
Aor13HI TCCGGA 1 cut(s) 366
AoxI GGCC 9 cut(s) 28, 33, 100, 127, 316, 621, 906, 939, 996
ApeKI GCWGC 4 cut(s) 271, 580, 853, 1277
Asp718I GGTACC 1 cut(s) 431
AspLEI GCGC 3 cut(s) 173, 192, 194
AspS9I GGNCC 1 cut(s) 232
AsuC2I CCSGG 2 cut(s) 391, 436
AsuHPI GGTGA 4 cut(s) 712, 865, 1109, 1241
AvaI CYCGRG 4 cut(s) 77, 209, 266, 446
AvaII GGWCC 1 cut(s) 232
AxyI CCTNAGG 3 cut(s) 525, 725, 975
BaeGI GKGCMC 1 cut(s) 1043
BalI TGGCCA 2 cut(s) 908, 998
BamHI GGATCC 3 cut(s) 87, 363, 792
BanI GGYRCC 5 cut(s) 170, 431, 970, 1038, 1307
BanII GRGCYC 3 cut(s) 216, 396, 742
BauI CACGAG 2 cut(s) 848, 1263
BbsI GAAGAC 1 cut(s) 403
Bbv12I GWGCWC 2 cut(s) 567, 591
BbvCI CCTCAGC 2 cut(s) 1155, 1320
BbvI GCAGC 4 cut(s) 283, 567, 865, 1264
BccI CCATC 6 cut(s) 203, 335, 728, 898, 1010, 1325
BceAI ACGGC 2 cut(s) 115, 404
BciT130I CCWGG 7 cut(s) 32, 44, 356, 398, 407, 650, 1092
BclI TGATCA 1 cut(s) 1174
BcnI CCSGG 2 cut(s) 391, 436
BcoDI GTCTC 5 cut(s) 80, 158, 449, 697, 1338
BfoI RGCGCY 1 cut(s) 174
BmcAI AGTACT 1 cut(s) 1352
Bme1390I CCNGG 9 cut(s) 32, 44, 356, 391, 398, 407, 436, 650, 1092
Bme18I GGWCC 1 cut(s) 232
BmeT110I CYCGRG 4 cut(s) 77, 209, 266, 446
BmgBI CACGTC 1 cut(s) 184
BmgT120I GGNCC 1 cut(s) 232
BmrFI CCNGG 9 cut(s) 32, 44, 356, 391, 398, 407, 436, 650, 1092
BmrI ACTGGG 1 cut(s) 1056
BmsI GCATC 1 cut(s) 96
BmuI ACTGGG 1 cut(s) 1056
BpiI GAAGAC 1 cut(s) 403
BplI GAGNNNNNCTC 4 cut(s) 842, 874, 1148, 1180
BpmI CTGGAG 3 cut(s) 380, 482, 1304
Bpu10I CCTNAGC 3 cut(s) 1107, 1155, 1320
BpuEI CTTGAG 1 cut(s) 605
BpuMI CCSGG 2 cut(s) 391, 436
BsaBI GATNNNNATC 1 cut(s) 23
BsaHI GRCGYC 3 cut(s) 141, 171, 294
BsaI GGTCTC 2 cut(s) 158, 449
BsaJI CCNNGG 6 cut(s) 130, 397, 648, 788, 925, 958
BsaWI WCCGGW 2 cut(s) 239, 366
BsaXI ACNNNNNCTCC 2 cut(s) 954, 984
Bsc4I CCNNNNNNNGG 4 cut(s) 649, 726, 926, 1108
Bse118I RCCGGY 2 cut(s) 167, 173
Bse1I ACTGG 3 cut(s) 1040, 1051, 1146
Bse21I CCTNAGG 3 cut(s) 525, 725, 975
Bse8I GATNNNNATC 1 cut(s) 23
BseAI TCCGGA 1 cut(s) 366
BseBI CCWGG 7 cut(s) 32, 44, 356, 398, 407, 650, 1092
BseDI CCNNGG 6 cut(s) 130, 397, 648, 788, 925, 958
BseGI GGATG 2 cut(s) 757, 985
BseJI GATNNNNATC 1 cut(s) 23
BseLI CCNNNNNNNGG 4 cut(s) 649, 726, 926, 1108
BseMII CTCAG 9 cut(s) 539, 710, 716, 750, 858, 873, 1146, 1248, 1311
BseNI ACTGG 3 cut(s) 1040, 1051, 1146
BsePI GCGCGC 1 cut(s) 190
BseRI GAGGAG 4 cut(s) 513, 799, 975, 1313
BseSI GKGCMC 1 cut(s) 1043
BseX3I CGGCCG 1 cut(s) 127
BseXI GCAGC 4 cut(s) 283, 567, 865, 1264
BseYI CCCAGC 1 cut(s) 60
BsgI GTGCAG 2 cut(s) 370, 1133
Bsh1236I CGCG 4 cut(s) 132, 190, 192, 315
Bsh1285I CGRYCG 1 cut(s) 130
BshFI GGCC 9 cut(s) 30, 35, 102, 129, 318, 623, 908, 941, 998
BshNI GGYRCC 5 cut(s) 170, 431, 970, 1038, 1307
BsiEI CGRYCG 1 cut(s) 130
BsiHKAI GWGCWC 2 cut(s) 567, 591
BsiHKCI CYCGRG 4 cut(s) 77, 209, 266, 446
BsiSI CCGG 9 cut(s) 36, 95, 168, 174, 240, 282, 367, 390, 435
BslFI GGGAC 2 cut(s) 170, 648
BslI CCNNNNNNNGG 4 cut(s) 649, 726, 926, 1108
BsmAI GTCTC 5 cut(s) 80, 158, 449, 697, 1338
BsmBI CGTCTC 2 cut(s) 697, 1338
BsmFI GGGAC 2 cut(s) 170, 648
BsmI GAATGC 1 cut(s) 991
BsnI GGCC 9 cut(s) 30, 35, 102, 129, 318, 623, 908, 941, 998
Bso31I GGTCTC 2 cut(s) 158, 449
BsoBI CYCGRG 4 cut(s) 77, 209, 266, 446
Bsp1286I GDGCHC 6 cut(s) 216, 396, 567, 591, 742, 1043
Bsp13I TCCGGA 1 cut(s) 366
Bsp19I CCATGG 1 cut(s) 925
BspANI GGCC 9 cut(s) 30, 35, 102, 129, 318, 623, 908, 941, 998
BspCNI CTCAG 9 cut(s) 538, 709, 717, 751, 859, 872, 1147, 1249, 1312
BspEI TCCGGA 1 cut(s) 366
BspFNI CGCG 4 cut(s) 132, 190, 192, 315
BspQI GCTCTTC 1 cut(s) 263
BspT107I GGYRCC 5 cut(s) 170, 431, 970, 1038, 1307
BspTNI GGTCTC 2 cut(s) 158, 449
BsrFI RCCGGY 2 cut(s) 167, 173
BsrI ACTGG 3 cut(s) 1040, 1051, 1146
BssAI RCCGGY 2 cut(s) 167, 173
BssECI CCNNGG 6 cut(s) 130, 397, 648, 788, 925, 958
BssHII GCGCGC 1 cut(s) 190
BssNI GRCGYC 3 cut(s) 141, 171, 294
BssSI CACGAG 2 cut(s) 848, 1263
BssT1I CCWWGG 2 cut(s) 788, 925
Bst2BI CACGAG 2 cut(s) 848, 1263
Bst2UI CCWGG 7 cut(s) 32, 44, 356, 398, 407, 650, 1092
Bst4CI ACNGT 7 cut(s) 74, 124, 220, 511, 560, 881, 1084
Bst6I CTCTTC 1 cut(s) 263
BstACI GRCGYC 3 cut(s) 141, 171, 294
BstDSI CCRYGG 2 cut(s) 130, 925
BstEII GGTNACC 1 cut(s) 1097
BstENI CCTNNNNNAGG 2 cut(s) 724, 1106
BstF5I GGATG 2 cut(s) 757, 985
BstFNI CGCG 4 cut(s) 132, 190, 192, 315
BstH2I RGCGCY 1 cut(s) 174
BstHHI GCGC 3 cut(s) 173, 192, 194
BstMAI GTCTC 5 cut(s) 80, 158, 449, 697, 1338
BstMCI CGRYCG 1 cut(s) 130
BstMWI GCNNNNNNNGC 7 cut(s) 187, 466, 586, 839, 995, 1205, 1316
BstNI CCWGG 7 cut(s) 32, 44, 356, 398, 407, 650, 1092
BstPI GGTNACC 1 cut(s) 1097
BstSCI CCNGG 9 cut(s) 30, 42, 354, 389, 396, 405, 434, 648, 1090
BstSLI GKGCMC 1 cut(s) 1043
BstUI CGCG 4 cut(s) 132, 190, 192, 315
BstV1I GCAGC 4 cut(s) 283, 567, 865, 1264
BstV2I GAAGAC 1 cut(s) 403
BstX2I RGATCY 4 cut(s) 87, 363, 792, 1270
BstXI CCANNNNNNTGG 1 cut(s) 1150
BstYI RGATCY 4 cut(s) 87, 363, 792, 1270
BstZI CGGCCG 1 cut(s) 127
Bsu36I CCTNAGG 3 cut(s) 525, 725, 975
BsuRI GGCC 9 cut(s) 30, 35, 102, 129, 318, 623, 908, 941, 998
BtgI CCRYGG 2 cut(s) 130, 925
BtgZI GCGATG 1 cut(s) 1350
BtrI CACGTC 1 cut(s) 184
BtsCI GGATG 2 cut(s) 757, 985
BtsIMutI CAGTG 6 cut(s) 507, 565, 867, 886, 1080, 1153
CaiI CAGNNNCTG 1 cut(s) 866
CfoI GCGC 3 cut(s) 173, 192, 194
Cfr10I RCCGGY 2 cut(s) 167, 173
Cfr13I GGNCC 1 cut(s) 232
Cfr42I CCGCGG 1 cut(s) 133
CseI GACGC 3 cut(s) 149, 302, 650
Csp6I GTAC 5 cut(s) 432, 934, 955, 1017, 1351
CspCI CAANNNNNGTGG 2 cut(s) 523, 558
CviAII CATG 7 cut(s) 83, 802, 901, 926, 937, 1129, 1237
CviQI GTAC 5 cut(s) 432, 934, 955, 1017, 1351
DinI GGCGCC 1 cut(s) 172
DrdI GACNNNNNNGTC 1 cut(s) 122
DseDI GACNNNNNNGTC 1 cut(s) 122
EaeI YGGCCR 6 cut(s) 28, 100, 127, 316, 906, 996
EagI CGGCCG 1 cut(s) 127
Eam1104I CTCTTC 1 cut(s) 263
EarI CTCTTC 1 cut(s) 263
EclXI CGGCCG 1 cut(s) 127
Eco130I CCWWGG 2 cut(s) 788, 925
Eco24I GRGCYC 3 cut(s) 216, 396, 742
Eco31I GGTCTC 2 cut(s) 158, 449
Eco47I GGWCC 1 cut(s) 232
Eco52I CGGCCG 1 cut(s) 127
Eco81I CCTNAGG 3 cut(s) 525, 725, 975
Eco88I CYCGRG 4 cut(s) 77, 209, 266, 446
Eco91I GGTNACC 1 cut(s) 1097
EcoNI CCTNNNNNAGG 2 cut(s) 724, 1106
EcoO65I GGTNACC 1 cut(s) 1097
EcoRII CCWGG 7 cut(s) 30, 42, 354, 396, 405, 648, 1090
EcoT14I CCWWGG 2 cut(s) 788, 925
EcoT38I GRGCYC 3 cut(s) 216, 396, 742
EgeI GGCGCC 1 cut(s) 172
EheI GGCGCC 1 cut(s) 172
ErhI CCWWGG 2 cut(s) 788, 925
Esp3I CGTCTC 2 cut(s) 697, 1338
FaeI CATG 7 cut(s) 86, 805, 904, 929, 940, 1132, 1240
FaqI GGGAC 2 cut(s) 170, 648
FatI CATG 7 cut(s) 82, 801, 900, 925, 936, 1128, 1236
FauI CCCGC 2 cut(s) 195, 1175
FbaI TGATCA 1 cut(s) 1174
FblI GTMKAC 1 cut(s) 204
FokI GGATG 2 cut(s) 764, 992
FriOI GRGCYC 3 cut(s) 216, 396, 742
GlaI GCGC 3 cut(s) 172, 191, 193
GsaI CCCAGC 1 cut(s) 64
GsuI CTGGAG 3 cut(s) 380, 482, 1304
HaeII RGCGCY 1 cut(s) 174
HaeIII GGCC 9 cut(s) 30, 35, 102, 129, 318, 623, 908, 941, 998
HapII CCGG 9 cut(s) 36, 95, 168, 174, 240, 282, 367, 390, 435
HgaI GACGC 3 cut(s) 149, 302, 650
HhaI GCGC 3 cut(s) 173, 192, 194
Hin1I GRCGYC 3 cut(s) 141, 171, 294
Hin1II CATG 7 cut(s) 86, 805, 904, 929, 940, 1132, 1240
Hin6I GCGC 3 cut(s) 171, 190, 192
HinP1I GCGC 3 cut(s) 171, 190, 192
HincII GTYRAC 1 cut(s) 205
HindII GTYRAC 1 cut(s) 205
HinfI GANTC 4 cut(s) 39, 402, 504, 1313
HpaII CCGG 9 cut(s) 36, 95, 168, 174, 240, 282, 367, 390, 435
HphI GGTGA 4 cut(s) 712, 865, 1109, 1241
Hpy166II GTNNAC 3 cut(s) 205, 478, 1033
Hpy188I TCNGA 7 cut(s) 50, 163, 363, 503, 744, 1191, 1258
Hpy188III TCNNGA 7 cut(s) 77, 331, 367, 766, 1006, 1265, 1283
Hpy8I GTNNAC 3 cut(s) 205, 478, 1033
Hpy99I CGWCG 7 cut(s) 128, 140, 143, 206, 230, 347, 425
HpyAV CCTTC 6 cut(s) 154, 163, 528, 1238, 1282, 1334
HpyCH4III ACNGT 7 cut(s) 74, 124, 220, 511, 560, 881, 1084
HpyCH4IV ACGT 1 cut(s) 183
HpyCH4V TGCA 5 cut(s) 12, 387, 1114, 1128, 1216
HpyF10VI GCNNNNNNNGC 7 cut(s) 187, 466, 586, 839, 995, 1205, 1316
HpySE526I ACGT 1 cut(s) 183
Hsp92I GRCGYC 3 cut(s) 141, 171, 294
Hsp92II CATG 7 cut(s) 86, 805, 904, 929, 940, 1132, 1240
HspAI GCGC 3 cut(s) 171, 190, 192
KasI GGCGCC 1 cut(s) 170
Kpn2I TCCGGA 1 cut(s) 366
KpnI GGTACC 1 cut(s) 435
KroI GCCGGC 1 cut(s) 173
KroNI GCCGGC 1 cut(s) 175
Ksp22I TGATCA 1 cut(s) 1174
KspI CCGCGG 1 cut(s) 133
LguI GCTCTTC 1 cut(s) 263
LmnI GCTCC 4 cut(s) 64, 399, 547, 823
Lsp1109I GCAGC 4 cut(s) 283, 567, 865, 1264
LweI GCATC 1 cut(s) 96
MaeII ACGT 1 cut(s) 183
MaeIII GTNAC 2 cut(s) 754, 1097
MboII GAAGA 4 cut(s) 148, 190, 250, 403
MflI RGATCY 4 cut(s) 87, 363, 792, 1270
MhlI GDGCHC 6 cut(s) 216, 396, 567, 591, 742, 1043
MlsI TGGCCA 2 cut(s) 908, 998
MluNI TGGCCA 2 cut(s) 908, 998
Mly113I GGCGCC 1 cut(s) 171
MlyI GAGTC 1 cut(s) 1322
MmeI TCCRAC 2 cut(s) 28, 1161
Mox20I TGGCCA 2 cut(s) 908, 998
MroI TCCGGA 1 cut(s) 366
MroNI GCCGGC 1 cut(s) 173
MscI TGGCCA 2 cut(s) 908, 998
MslI CAYNNNNRTG 4 cut(s) 594, 596, 1148, 1221
Msp20I TGGCCA 2 cut(s) 908, 998
MspA1I CMGCKG 1 cut(s) 132
MspI CCGG 9 cut(s) 36, 95, 168, 174, 240, 282, 367, 390, 435
MspR9I CCNGG 9 cut(s) 32, 44, 356, 391, 398, 407, 436, 650, 1092
Mva1269I GAATGC 1 cut(s) 991
MvaI CCWGG 7 cut(s) 32, 44, 356, 398, 407, 650, 1092
MvnI CGCG 4 cut(s) 132, 190, 192, 315
MwoI GCNNNNNNNGC 7 cut(s) 187, 466, 586, 839, 995, 1205, 1316
NaeI GCCGGC 1 cut(s) 175
NarI GGCGCC 1 cut(s) 171
NciI CCSGG 2 cut(s) 391, 436
NcoI CCATGG 1 cut(s) 925
NgoMIV GCCGGC 1 cut(s) 173
NlaIII CATG 7 cut(s) 86, 805, 904, 929, 940, 1132, 1240
NmeAIII GCCGAG 2 cut(s) 135, 1336
NmuCI GTSAC 2 cut(s) 754, 1097
OliI CACNNNNGTG 2 cut(s) 596, 1148
PaeR7I CTCGAG 2 cut(s) 77, 209
PauI GCGCGC 1 cut(s) 190
PciSI GCTCTTC 1 cut(s) 263
PcsI WCGNNNNNNNCGW 3 cut(s) 132, 207, 216
PctI GAATGC 1 cut(s) 991
PdiI GCCGGC 1 cut(s) 175
PfeI GAWTC 3 cut(s) 39, 402, 504
PflFI GACNNNGTC 1 cut(s) 230
PleI GAGTC 1 cut(s) 1321
PluTI GGCGCC 1 cut(s) 174
PpsI GAGTC 1 cut(s) 1321
Psp6I CCWGG 7 cut(s) 30, 42, 354, 396, 405, 648, 1090
PspEI GGTNACC 1 cut(s) 1097
PspFI CCCAGC 1 cut(s) 60
PspGI CCWGG 7 cut(s) 30, 42, 354, 396, 405, 648, 1090
PspPI GGNCC 1 cut(s) 232
PspXI VCTCGAGB 1 cut(s) 209
PstNI CAGNNNCTG 1 cut(s) 866
PsuI RGATCY 4 cut(s) 87, 363, 792, 1270
PsyI GACNNNGTC 1 cut(s) 230
PteI GCGCGC 1 cut(s) 190
RsaI GTAC 5 cut(s) 433, 935, 956, 1018, 1352
RsaNI GTAC 5 cut(s) 432, 934, 955, 1017, 1351
RseI CAYNNNNRTG 4 cut(s) 594, 596, 1148, 1221
SacII CCGCGG 1 cut(s) 133
SalI GTCGAC 1 cut(s) 203
SapI GCTCTTC 1 cut(s) 263
Sau96I GGNCC 1 cut(s) 232
ScaI AGTACT 1 cut(s) 1352
SchI GAGTC 1 cut(s) 1322
ScrFI CCNGG 9 cut(s) 32, 44, 356, 391, 398, 407, 436, 650, 1092
SduI GDGCHC 6 cut(s) 216, 396, 567, 591, 742, 1043
SfaNI GCATC 1 cut(s) 96
SfoI GGCGCC 1 cut(s) 172
Sfr274I CTCGAG 2 cut(s) 77, 209
Sfr303I CCGCGG 1 cut(s) 133
SgrBI CCGCGG 1 cut(s) 133
SinI GGWCC 1 cut(s) 232
SlaI CTCGAG 2 cut(s) 77, 209
SmiMI CAYNNNNRTG 4 cut(s) 594, 596, 1148, 1221
SmlI CTYRAG 3 cut(s) 77, 209, 584
SmoI CTYRAG 3 cut(s) 77, 209, 584
SspDI GGCGCC 1 cut(s) 170
StyD4I CCNGG 9 cut(s) 30, 42, 354, 389, 396, 405, 434, 648, 1090
StyI CCWWGG 2 cut(s) 788, 925
TaaI ACNGT 7 cut(s) 74, 124, 220, 511, 560, 881, 1084
TaiI ACGT 1 cut(s) 186
TaqI TCGA 7 cut(s) 17, 78, 204, 210, 225, 345, 612
TatI WGTACW 2 cut(s) 933, 1350
TauI GCSGC 6 cut(s) 132, 135, 299, 318, 642, 992
TfiI GAWTC 3 cut(s) 39, 402, 504
TscAI CASTG 6 cut(s) 514, 565, 867, 886, 1087, 1153
TseFI GTSAC 2 cut(s) 754, 1097
TseI GCWGC 4 cut(s) 271, 580, 853, 1277
Tsp45I GTSAC 2 cut(s) 754, 1097
TspDTI ATGAA 3 cut(s) 696, 790, 1353
TspRI CASTG 6 cut(s) 514, 565, 867, 886, 1087, 1153
Tth111I GACNNNGTC 1 cut(s) 230
VpaK11BI GGWCC 1 cut(s) 232
XagI CCTNNNNNAGG 2 cut(s) 724, 1106
XcmI CCANNNNNNNNNTGG 2 cut(s) 541, 1027
XhoI CTCGAG 2 cut(s) 77, 209
XmiI GTMKAC 1 cut(s) 204
ZrmI AGTACT 1 cut(s) 1352
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.