Rmu_sc0011069.1_g000003

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0011069.1
OG0000549
Physical Location & Seq
Forward (+)
8282 .. 13201
4920 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0011069.1_g000003.1.cds

Sequence Viewer

Length: 2517 bp
atgtatgtactcccgcttgcttttacaatgtttctcagtggaagtgagtatgttccatcggggtgggaagatgaattgcttttaacagatgatactttccttggagatggatttggtgttgggattgatggagaagagggtatgtccaacaatcttaataccacttcttgcaatactgaagatagaagtggtattgctagttcttcttcaaaaccatcaaaagccaaaatgttgtcccaagaaaccatatccgaatacttttacatgccgataactcgagcagctgaagaaatgaatgtctctgtaacaactcttaagaaaaggtgtcgagagttgggtattcatcgttggccttgccgaaagttgaacagcgtaaagaacctagaagaaatcattcaggccctgtacagaagcgctcaggcatatatccagctggcagatttgtatttggctaagcttgacatcaagaaagatcatgaaatagaccctaacaacataaatgtgcagttggagtacaaaacttcgaaggagaagatgaaaggaggcaaagttttatggcaacatgcgggttacggcggtgggatatccggcaactattactgcgctgggtcgtatcatctccaaggtaactcctataaccaagctcagatgttcttcccacctccatacttgcaatcgattcttcccattgctccgcagtcactcaccataactcctcaaaccccatcaatccctccttaccactcacctcatcctaccccaaatatctggattcatccttcaattccaaactttcagtcgcctgctgccccaacttttatgccccagcctcagtttccaaatcctaaacctccatcgtcttttcgttccgtgggcgattgtgagcccctagctctattcacgggttggatccaaaccataccttcttcctcttctttatcatctgacccacaaacctcccttgcagaggcaacaaatccaacttctttgcatcacaagtcgtttcggcgttccagacatagagtcgccatggcggcgaaagtgagagttgctaaaaagacccgaaacccatatatgctccatggtgtgggtaagtcctcgaggtctaagatgtaccacaagcgcaacctcttcccacgtcacaactccaaggcaacaaatccaacttctccaacgccaatttcaagtgttccggagaaaatggaggttttccattccacgctccccgtctccgccatcatggacgggctcgagaagctacgccgtcaggaaaacaacattccgcctggccaaatgcagagtgacaagacagtcgggggcagagacgacgccttcaacactttcttcaatgaaaccggagccggattgcacatcccccgcgccgtgttcctggatctggagcccaccgtcatcaacgaggtgaggactggaacttaccagctgttccaccccgagcagctcatcagcggcaaagaggacgccactaataacttcaccagaggccattacacaattgggaaggagattgtggatcggatccggaagcttgcggacaactgtactgggcttcaatggtttctagtgtcccatgccgtcagaggtgggactggatctgggcttggatctctgctcttggagaggctctctgtggactatgggaataaatctaagcttggattcactgtttacccatctcctcaggtctcaacctctgttgttgagccttataacaatgttttgtccactcgctcgctctgggagcacactaatgtggctgtgcttctggacaatgaagccatctatgatatctgccgcagattgcttgacattgagcgacctacgtacaccaatctcaacaggcttgtttctcaggtcatttcctcactcactgcttctctgctatttgatccagcgtcaggtgttcaagccccattagaatgtcatccattatcagtagttagaattgatgagtgcttgtcttcgcttgattttgttctgattgttgagaagaatgagaagttttcaagtcatagtcctgccatgaattttgcaaattccttggagactttagaagatgttaaaaaattgtttctgaaaatgcctgaaaggaacattgtttcatggaatactttgattagtgcgttggtccaaattggtcaggaggaaaaggcaatgcctgtttattatgctttggtttcggagggagtggtgccacctaattccaatctctacatgatgcctccttatgatttcaaggttgataatcacagatgtggtttccaaagaattttaattgaaccgctgattgctttctacttgagatgcataaataagcagtccagccttggaaggtatgatgatagtcatcctaaagtttcatctacttttttgaatgaagaagaatatatgtttgtgataattattaatgtgtgcactgctttagttcttaagtattgtccaactggtatggtggaagtatcagagaagtggtttatggacatggtggatatgaattag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04145 ko04210 ko04530 ko04540 ko05130 map04145 map04210 map04530 map04540 map05130
Pfam Domains
Protein Families
Tubulin Tubulin_C

Protein Analysis

838

Amino Acids

93.86

Weight (kDa)

6.91

Isoelectric Point (pI)

57.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000549)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04820 AT1G50010 AT4G14960 AT4G14960
fragaria_vesca FvH4_1g18660 FvH4_2g03140 FvH4_5g33560 FvH4_6g48940 FvH4_6g48940
malus_domestica MD02G1204800.v1.1 MD05G1106800.v1.1 MD08G1210600.v1.1 MD09G1049500.v1.1 MD10G1111600.v1.1 MD15G1398700.v1.1 MD17G1049700.v1.1
prunus_persica Prupe.1G542600_v2.0.a1 Prupe.3G269300_v2.0.a1 Prupe.6G212700_v2.0.a1 Prupe.8G150900_v2.0.a1
pyrus_communis pycom05g10370 pycom08g18150 pycom10g09640 pycom10g09660 pycom111g04050 pycom15g35470 pycom17g04460
rosa_chinensis RchiOBHm_Chr2g0109481 RchiOBHm_Chr2g0168751 RchiOBHm_Chr3g0453071 RchiOBHm_Chr3g0460741 RchiOBHm_Chr3g0467971 RchiOBHm_Chr4g0400901 RchiOBHm_Chr6g0248011 RchiOBHm_Chr7g0232381 RchiOBHm_Chr7g0238121
rosa_laevigata RLG00000001350 RLG00000009167 RLG00000015163 RLG00000017708 RLG00000017741 RLG00000021807 RLG00000023849 RLG00000026518
rosa_multiflora Rmu_sc0000332.1_g000021 Rmu_sc0000332.1_g000029 Rmu_sc0002416.1_g000007 Rmu_sc0002611.1_g000001 Rmu_sc0003113.1_g000016 Rmu_sc0004780.1_g000001 Rmu_sc0004805.1_g000035 Rmu_sc0010633.1_g000010 Rmu_sc0011069.1_g000003 Rmu_sc0016170.1_g000002 Rmu_sc0025757.1_g000003 Rmu_ssc0000484.1_g000003
rosa_roxburghii Rroxscaffold_2G00134200 Rroxscaffold_4G00321030 Rroxscaffold_7G00213500
rosa_rugosa Rorug01G0283100 Rorug02G0160500 Rorug02G0535300 Rorug04G0027000 Rorug04G0328100 Rorug05G0388700 Rorug05G0530200 Rorug06G0489200 Rorug07G0104900 Rorug07G0209400 Rorug07G0274500
rosa_samantha Rh2BG222800 Rh2BG618900 Rh2CG214400 Rh2CG587200 Rh6BG040200 Rh6CG206500 Rh6DG035600 Rh7BG401600 Rh7BG401700 Rh7DG418400
rosa_wichuraiana Rw2G050240 Rw6G003920 Rw7G035430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1728
AasI GACNNNNNNGTC 1 cut(s) 1310
AbsI CCTCGAGG 1 cut(s) 1099
AccB1I GGYRCC 1 cut(s) 2209
AccB7I CCANNNNNTGG 1 cut(s) 1087
AccII CGCG 1 cut(s) 1380
AccIII TCCGGA 2 cut(s) 1192, 1539
AclWI GGATC 9 cut(s) 904, 917, 1401, 1531, 1539, 1544, 1618, 1630, 1901
AcoI YGGCCR 1 cut(s) 1288
AcsI RAATTY 3 cut(s) 2044, 2053, 2286
AcuI CTGAAG 2 cut(s) 198, 306
AcyI GRCGYC 2 cut(s) 1329, 1479
AfaI GTAC 6 cut(s) 9, 407, 515, 1115, 1561, 1844
AfeI AGCGCT 1 cut(s) 415
AfiI CCNNNNNNNGG 4 cut(s) 967, 1087, 1396, 1916
AflII CTTAAG 2 cut(s) 314, 2447
AhdI GACNNNNNGTC 1 cut(s) 1022
AjiI CACGTC 1 cut(s) 1139
AjnI CCWGG 2 cut(s) 1285, 1389
AjuI GAANNNNNNNTTGG 2 cut(s) 1165, 1197
AleI CACNNNNGTG 2 cut(s) 1769, 2271
Alw21I GWGCWC 2 cut(s) 1764, 2435
Alw26I GTCTC 5 cut(s) 304, 1234, 1317, 1708, 2057
Alw44I GTGCAC 1 cut(s) 2431
AlwI GGATC 9 cut(s) 904, 917, 1401, 1531, 1539, 1544, 1618, 1630, 1901
AlwNI CAGNNNCTG 1 cut(s) 403
Ama87I CYCGRG 4 cut(s) 276, 1099, 1250, 1451
Aor13HI TCCGGA 2 cut(s) 1192, 1539
Aor51HI AGCGCT 1 cut(s) 415
AoxI GGCC 4 cut(s) 350, 399, 1288, 1501
ApaLI GTGCAC 1 cut(s) 2431
ApeKI GCWGC 3 cut(s) 281, 806, 1456
ApoI RAATTY 3 cut(s) 2044, 2053, 2286
ArsI GACNNNNNNTTYG 2 cut(s) 782, 814
AseI ATTAAT 1 cut(s) 2424
Asp700I GAANNNNTTC 1 cut(s) 393
AspLEI GCGC 4 cut(s) 416, 605, 1125, 1382
AspS9I GGNCC 2 cut(s) 400, 2146
AsuHPI GGTGA 4 cut(s) 697, 738, 1432, 1486
AsuII TTCGAA 1 cut(s) 524
AvaI CYCGRG 4 cut(s) 276, 1099, 1250, 1451
AvaII GGWCC 1 cut(s) 2146
AxyI CCTNAGG 1 cut(s) 1698
BaeGI GKGCMC 1 cut(s) 2435
BalI TGGCCA 1 cut(s) 1290
BamHI GGATCC 2 cut(s) 909, 1536
BanI GGYRCC 1 cut(s) 2209
BanII GRGCYC 3 cut(s) 888, 1251, 1404
BbsI GAAGAC 1 cut(s) 1971
Bbv12I GWGCWC 2 cut(s) 1764, 2435
BbvI GCAGC 3 cut(s) 293, 793, 1468
BccI CCATC 9 cut(s) 64, 101, 122, 223, 733, 862, 1244, 1699, 1805
BceAI ACGGC 4 cut(s) 589, 1248, 1367, 1577
BciT130I CCWGG 2 cut(s) 1287, 1391
BcoDI GTCTC 5 cut(s) 304, 1234, 1317, 1708, 2057
BfaI CTAG 4 cut(s) 198, 383, 890, 1580
BfoI RGCGCY 1 cut(s) 417
BfrI CTTAAG 2 cut(s) 314, 2447
BglI GCCNNNNNGGC 1 cut(s) 1034
BisI GCNGC 6 cut(s) 282, 807, 1035, 1457, 1468, 1813
BlpI GCTNAGC 1 cut(s) 453
BlsI GCNGC 6 cut(s) 283, 808, 1036, 1458, 1469, 1814
Bme1390I CCNGG 2 cut(s) 1287, 1391
Bme18I GGWCC 1 cut(s) 2146
BmeRI GACNNNNNGTC 1 cut(s) 1022
BmeT110I CYCGRG 4 cut(s) 276, 1099, 1250, 1451
BmgBI CACGTC 1 cut(s) 1139
BmgT120I GGNCC 2 cut(s) 400, 2146
BmiI GGNNCC 5 cut(s) 911, 1360, 1401, 1538, 2211
BmrFI CCNGG 2 cut(s) 1287, 1391
BmrI ACTGGG 1 cut(s) 1572
BmsI GCATC 3 cut(s) 1000, 2226, 2312
BmuI ACTGGG 1 cut(s) 1572
BpiI GAAGAC 1 cut(s) 1971
BplI GAGNNNNNCTC 2 cut(s) 1628, 1660
BpmI CTGGAG 1 cut(s) 1418
Bpu10I CCTNAGC 1 cut(s) 417
Bpu1102I GCTNAGC 1 cut(s) 453
Bpu14I TTCGAA 1 cut(s) 524
BpuEI CTTGAG 1 cut(s) 2338
Bsa29I ATCGAT 1 cut(s) 677
BsaAI YACGTR 1 cut(s) 1842
BsaBI GATNNNNATC 1 cut(s) 2364
BsaHI GRCGYC 2 cut(s) 1329, 1479
BsaI GGTCTC 1 cut(s) 1708
BsaJI CCNNGG 8 cut(s) 100, 622, 870, 1029, 1081, 1149, 2058, 2344
BsaWI WCCGGW 3 cut(s) 1192, 1355, 1539
BsaXI ACNNNNNCTCC 4 cut(s) 534, 564, 697, 727
Bsc4I CCNNNNNNNGG 4 cut(s) 967, 1087, 1396, 1916
Bse1I ACTGG 4 cut(s) 1432, 1567, 1612, 2467
Bse21I CCTNAGG 1 cut(s) 1698
Bse3DI GCAATG 2 cut(s) 687, 2178
Bse8I GATNNNNATC 1 cut(s) 2364
BseAI TCCGGA 2 cut(s) 1192, 1539
BseBI CCWGG 2 cut(s) 1287, 1391
BseCI ATCGAT 1 cut(s) 677
BseDI CCNNGG 8 cut(s) 100, 622, 870, 1029, 1081, 1149, 2058, 2344
BseGI GGATG 5 cut(s) 751, 775, 1371, 1942, 2365
BseJI GATNNNNATC 1 cut(s) 2364
BseLI CCNNNNNNNGG 4 cut(s) 967, 1087, 1396, 1916
BseMI GCAATG 2 cut(s) 687, 2178
BseMII CTCAG 6 cut(s) 49, 431, 659, 845, 1712, 1883
BseNI ACTGG 4 cut(s) 1432, 1567, 1612, 2467
BseRI GAGGAG 2 cut(s) 705, 1686
BseSI GKGCMC 1 cut(s) 2435
BseXI GCAGC 3 cut(s) 293, 793, 1468
BseYI CCCAGC 2 cut(s) 605, 825
BsgI GTGCAG 1 cut(s) 524
Bsh1236I CGCG 1 cut(s) 1380
BshFI GGCC 4 cut(s) 352, 401, 1290, 1503
BshNI GGYRCC 1 cut(s) 2209
BshVI ATCGAT 1 cut(s) 677
BsiHKAI GWGCWC 2 cut(s) 1764, 2435
BsiHKCI CYCGRG 4 cut(s) 276, 1099, 1250, 1451
BsiSI CCGG 5 cut(s) 588, 1193, 1356, 1362, 1540
BslFI GGGAC 3 cut(s) 220, 1570, 1618
BslI CCNNNNNNNGG 4 cut(s) 967, 1087, 1396, 1916
BsmAI GTCTC 5 cut(s) 304, 1234, 1317, 1708, 2057
BsmBI CGTCTC 2 cut(s) 1234, 1317
BsmFI GGGAC 3 cut(s) 220, 1570, 1618
BsnI GGCC 4 cut(s) 352, 401, 1290, 1503
Bso31I GGTCTC 1 cut(s) 1708
BsoBI CYCGRG 4 cut(s) 276, 1099, 1250, 1451
Bsp119I TTCGAA 1 cut(s) 524
Bsp1286I GDGCHC 5 cut(s) 888, 1251, 1404, 1764, 2435
Bsp13I TCCGGA 2 cut(s) 1192, 1539
Bsp1407I TGTACA 1 cut(s) 405
Bsp143I GATC 8 cut(s) 472, 909, 1393, 1531, 1536, 1610, 1622, 1906
Bsp1720I GCTNAGC 1 cut(s) 453
Bsp19I CCATGG 2 cut(s) 1029, 1081
BspANI GGCC 4 cut(s) 352, 401, 1290, 1503
BspCNI CTCAG 6 cut(s) 48, 430, 658, 844, 1711, 1882
BspDI ATCGAT 1 cut(s) 677
BspEI TCCGGA 2 cut(s) 1192, 1539
BspFNI CGCG 1 cut(s) 1380
BspHI TCATGA 1 cut(s) 475
BspLI GGNNCC 5 cut(s) 911, 1360, 1401, 1538, 2211
BspPI GGATC 9 cut(s) 904, 917, 1401, 1531, 1539, 1544, 1618, 1630, 1901
BspT104I TTCGAA 1 cut(s) 524
BspT107I GGYRCC 1 cut(s) 2209
BspTI CTTAAG 2 cut(s) 314, 2447
BspTNI GGTCTC 1 cut(s) 1708
BsrDI GCAATG 2 cut(s) 687, 2178
BsrGI TGTACA 1 cut(s) 405
BsrI ACTGG 4 cut(s) 1432, 1567, 1612, 2467
BssECI CCNNGG 8 cut(s) 100, 622, 870, 1029, 1081, 1149, 2058, 2344
BssMI GATC 8 cut(s) 472, 909, 1393, 1531, 1536, 1610, 1622, 1906
BssNI GRCGYC 2 cut(s) 1329, 1479
BssT1I CCWWGG 7 cut(s) 100, 622, 1029, 1081, 1149, 2058, 2344
Bst2UI CCWGG 2 cut(s) 1287, 1391
Bst4CI ACNGT 4 cut(s) 1312, 1408, 1559, 1684
Bst6I CTCTTC 3 cut(s) 129, 937, 1136
BstACI GRCGYC 2 cut(s) 1329, 1479
BstAFI CTTAAG 2 cut(s) 314, 2447
BstAUI TGTACA 1 cut(s) 405
BstBAI YACGTR 1 cut(s) 1842
BstBI TTCGAA 1 cut(s) 524
BstC8I GCNNGC 5 cut(s) 18, 435, 804, 1548, 1751
BstDEI CTNAG 9 cut(s) 35, 417, 453, 645, 831, 1107, 1668, 1698, 1869
BstDSI CCRYGG 3 cut(s) 870, 1029, 1081
BstENI CCTNNNNNAGG 1 cut(s) 965
BstF5I GGATG 5 cut(s) 751, 775, 1371, 1942, 2365
BstFNI CGCG 1 cut(s) 1380
BstH2I RGCGCY 1 cut(s) 417
BstHHI GCGC 4 cut(s) 416, 605, 1125, 1382
BstKTI GATC 8 cut(s) 475, 912, 1396, 1534, 1539, 1613, 1625, 1909
BstMAI GTCTC 5 cut(s) 304, 1234, 1317, 1708, 2057
BstMBI GATC 8 cut(s) 472, 909, 1393, 1531, 1536, 1610, 1622, 1906
BstMWI GCNNNNNNNGC 3 cut(s) 1034, 1255, 1759
BstNI CCWGG 2 cut(s) 1287, 1391
BstNSI RCATGY 2 cut(s) 268, 566
BstSCI CCNGG 2 cut(s) 1285, 1389
BstSLI GKGCMC 1 cut(s) 2435
BstSNI TACGTA 1 cut(s) 1842
BstUI CGCG 1 cut(s) 1380
BstV1I GCAGC 3 cut(s) 293, 793, 1468
BstV2I GAAGAC 1 cut(s) 1971
BstX2I RGATCY 5 cut(s) 909, 1393, 1536, 1610, 1622
BstXI CCANNNNNNTGG 2 cut(s) 63, 768
BstYI RGATCY 5 cut(s) 909, 1393, 1536, 1610, 1622
Bsu15I ATCGAT 1 cut(s) 677
Bsu36I CCTNAGG 1 cut(s) 1698
BsuRI GGCC 4 cut(s) 352, 401, 1290, 1503
BsuTUI ATCGAT 1 cut(s) 677
BtgI CCRYGG 3 cut(s) 870, 1029, 1081
BtrI CACGTC 1 cut(s) 1139
BtsCI GGATG 5 cut(s) 751, 775, 1371, 1942, 2365
BtsI GCAGTG 2 cut(s) 1887, 2433
BtsIMutI CAGTG 4 cut(s) 43, 1680, 1887, 2433
Cac8I GCNNGC 5 cut(s) 18, 435, 804, 1548, 1751
CaiI CAGNNNCTG 1 cut(s) 403
CciI TCATGA 1 cut(s) 475
CfoI GCGC 4 cut(s) 416, 605, 1125, 1382
Cfr13I GGNCC 2 cut(s) 400, 2146
ClaI ATCGAT 1 cut(s) 677
CseI GACGC 3 cut(s) 1337, 1487, 1902
Csp6I GTAC 6 cut(s) 8, 406, 514, 1114, 1560, 1843
CviQI GTAC 6 cut(s) 8, 406, 514, 1114, 1560, 1843
DdeI CTNAG 9 cut(s) 35, 417, 453, 645, 831, 1107, 1668, 1698, 1869
DpnI GATC 8 cut(s) 474, 911, 1395, 1533, 1538, 1612, 1624, 1908
DpnII GATC 8 cut(s) 472, 909, 1393, 1531, 1536, 1610, 1622, 1906
DrdI GACNNNNNNGTC 1 cut(s) 1310
DriI GACNNNNNGTC 1 cut(s) 1022
DseDI GACNNNNNNGTC 1 cut(s) 1310
EaeI YGGCCR 1 cut(s) 1288
Eam1104I CTCTTC 3 cut(s) 129, 937, 1136
Eam1105I GACNNNNNGTC 1 cut(s) 1022
EarI CTCTTC 3 cut(s) 129, 937, 1136
EciI GGCGGA 2 cut(s) 1222, 1272
Eco105I TACGTA 1 cut(s) 1842
Eco130I CCWWGG 7 cut(s) 100, 622, 1029, 1081, 1149, 2058, 2344
Eco24I GRGCYC 3 cut(s) 888, 1251, 1404
Eco31I GGTCTC 1 cut(s) 1708
Eco32I GATATC 2 cut(s) 585, 1807
Eco47I GGWCC 1 cut(s) 2146
Eco47III AGCGCT 1 cut(s) 415
Eco57I CTGAAG 2 cut(s) 198, 306
Eco81I CCTNAGG 1 cut(s) 1698
Eco88I CYCGRG 4 cut(s) 276, 1099, 1250, 1451
EcoNI CCTNNNNNAGG 1 cut(s) 965
EcoO109I RGGNCCY 1 cut(s) 400
EcoRII CCWGG 2 cut(s) 1285, 1389
EcoRV GATATC 2 cut(s) 585, 1807
EcoT14I CCWWGG 7 cut(s) 100, 622, 1029, 1081, 1149, 2058, 2344
EcoT22I ATGCAT 1 cut(s) 2327
EcoT38I GRGCYC 3 cut(s) 888, 1251, 1404
ErhI CCWWGG 7 cut(s) 100, 622, 1029, 1081, 1149, 2058, 2344
Esp3I CGTCTC 2 cut(s) 1234, 1317
FaqI GGGAC 3 cut(s) 220, 1570, 1618
FauI CCCGC 3 cut(s) 21, 559, 1385
Fnu4HI GCNGC 6 cut(s) 282, 807, 1035, 1457, 1468, 1813
FokI GGATG 5 cut(s) 738, 762, 1358, 1929, 2352
FriOI GRGCYC 3 cut(s) 888, 1251, 1404
Fsp4HI GCNGC 6 cut(s) 282, 807, 1035, 1457, 1468, 1813
FspBI CTAG 4 cut(s) 198, 383, 890, 1580
GlaI GCGC 4 cut(s) 415, 604, 1124, 1381
GluI GCNGC 6 cut(s) 282, 807, 1035, 1457, 1468, 1813
GsaI CCCAGC 2 cut(s) 609, 829
GsuI CTGGAG 1 cut(s) 1418
HaeII RGCGCY 1 cut(s) 417
HaeIII GGCC 4 cut(s) 352, 401, 1290, 1503
HapII CCGG 5 cut(s) 588, 1193, 1356, 1362, 1540
HgaI GACGC 3 cut(s) 1337, 1487, 1902
HhaI GCGC 4 cut(s) 416, 605, 1125, 1382
Hin1I GRCGYC 2 cut(s) 1329, 1479
Hin6I GCGC 4 cut(s) 414, 603, 1123, 1380
HinP1I GCGC 4 cut(s) 414, 603, 1123, 1380
HindIII AAGCTT 3 cut(s) 455, 1544, 1670
HinfI GANTC 4 cut(s) 679, 772, 1023, 1677
HpaII CCGG 5 cut(s) 588, 1193, 1356, 1362, 1540
HphI GGTGA 4 cut(s) 697, 738, 1432, 1486
Hpy166II GTNNAC 5 cut(s) 1651, 1687, 1743, 1845, 2433
Hpy188I TCNGA 9 cut(s) 253, 648, 946, 1536, 1598, 1998, 2094, 2200, 2482
Hpy8I GTNNAC 5 cut(s) 1651, 1687, 1743, 1845, 2433
Hpy99I CGWCG 1 cut(s) 1331
HpyAV CCTTC 6 cut(s) 520, 789, 933, 1342, 1513, 2343
HpyCH4III ACNGT 4 cut(s) 1312, 1408, 1559, 1684
HpyCH4IV ACGT 2 cut(s) 1138, 1841
HpyF10VI GCNNNNNNNGC 3 cut(s) 1034, 1255, 1759
HpyF3I CTNAG 9 cut(s) 35, 417, 453, 645, 831, 1107, 1668, 1698, 1869
HpySE526I ACGT 2 cut(s) 1138, 1841
Hsp92I GRCGYC 2 cut(s) 1329, 1479
HspAI GCGC 4 cut(s) 414, 603, 1123, 1380
Kpn2I TCCGGA 2 cut(s) 1192, 1539
Kzo9I GATC 8 cut(s) 472, 909, 1393, 1531, 1536, 1610, 1622, 1906
LmnI GCTCC 6 cut(s) 697, 1083, 1227, 1358, 1399, 1759
Lsp1109I GCAGC 3 cut(s) 293, 793, 1468
LweI GCATC 3 cut(s) 1000, 2226, 2312
MaeI CTAG 4 cut(s) 198, 383, 890, 1580
MaeII ACGT 2 cut(s) 1138, 1841
MaeIII GTNAC 6 cut(s) 304, 569, 626, 699, 1139, 1301
MalI GATC 8 cut(s) 474, 911, 1395, 1533, 1538, 1612, 1624, 1908
MboI GATC 8 cut(s) 472, 909, 1393, 1531, 1536, 1610, 1622, 1906
MfeI CAATTG 1 cut(s) 1512
MflI RGATCY 5 cut(s) 909, 1393, 1536, 1610, 1622
MhlI GDGCHC 5 cut(s) 888, 1251, 1404, 1764, 2435
MlsI TGGCCA 1 cut(s) 1290
MluNI TGGCCA 1 cut(s) 1290
MlyI GAGTC 1 cut(s) 1032
MmeI TCCRAC 7 cut(s) 171, 489, 887, 1004, 1187, 1196, 2483
Mox20I TGGCCA 1 cut(s) 1290
Mph1103I ATGCAT 1 cut(s) 2327
MroI TCCGGA 2 cut(s) 1192, 1539
MroXI GAANNNNTTC 1 cut(s) 393
MscI TGGCCA 1 cut(s) 1290
MseI TTAA 7 cut(s) 83, 156, 315, 2079, 2291, 2424, 2448
MslI CAYNNNNRTG 6 cut(s) 61, 500, 1767, 1769, 1936, 2271
Msp20I TGGCCA 1 cut(s) 1290
MspA1I CMGCKG 5 cut(s) 284, 433, 1441, 1467, 2302
MspCI CTTAAG 2 cut(s) 314, 2447
MspI CCGG 5 cut(s) 588, 1193, 1356, 1362, 1540
MspR9I CCNGG 2 cut(s) 1287, 1391
MunI CAATTG 1 cut(s) 1512
MvaI CCWGG 2 cut(s) 1287, 1391
MvnI CGCG 1 cut(s) 1380
MwoI GCNNNNNNNGC 3 cut(s) 1034, 1255, 1759
NcoI CCATGG 2 cut(s) 1029, 1081
NdeII GATC 8 cut(s) 472, 909, 1393, 1531, 1536, 1610, 1622, 1906
NlaIV GGNNCC 5 cut(s) 911, 1360, 1401, 1538, 2211
NmuCI GTSAC 3 cut(s) 699, 1139, 1301
NsiI ATGCAT 1 cut(s) 2327
NspI RCATGY 2 cut(s) 268, 566
NspV TTCGAA 1 cut(s) 524
OliI CACNNNNGTG 2 cut(s) 1769, 2271
PaeR7I CTCGAG 3 cut(s) 276, 1099, 1250
PagI TCATGA 1 cut(s) 475
PdmI GAANNNNTTC 1 cut(s) 393
PfeI GAWTC 3 cut(s) 679, 772, 1677
PflMI CCANNNNNTGG 1 cut(s) 1087
PfoI TCCNGGA 1 cut(s) 1389
PkrI GCNGC 6 cut(s) 283, 808, 1036, 1458, 1469, 1814
PleI GAGTC 1 cut(s) 1031
PpsI GAGTC 1 cut(s) 1031
Ppu21I YACGTR 1 cut(s) 1842
PshBI ATTAAT 1 cut(s) 2424
PsiI TTATAA 1 cut(s) 1728
Psp6I CCWGG 2 cut(s) 1285, 1389
PspFI CCCAGC 2 cut(s) 605, 825
PspGI CCWGG 2 cut(s) 1285, 1389
PspN4I GGNNCC 5 cut(s) 911, 1360, 1401, 1538, 2211
PspPI GGNCC 2 cut(s) 400, 2146
PspXI VCTCGAGB 2 cut(s) 276, 1099
PsrI GAACNNNNNNTAC 2 cut(s) 184, 216
PstNI CAGNNNCTG 1 cut(s) 403
PsuI RGATCY 5 cut(s) 909, 1393, 1536, 1610, 1622
PvuII CAGCTG 3 cut(s) 284, 433, 1441
RsaI GTAC 6 cut(s) 9, 407, 515, 1115, 1561, 1844
RsaNI GTAC 6 cut(s) 8, 406, 514, 1114, 1560, 1843
RseI CAYNNNNRTG 6 cut(s) 61, 500, 1767, 1769, 1936, 2271
SaqAI TTAA 7 cut(s) 83, 156, 315, 2079, 2291, 2424, 2448
SatI GCNGC 6 cut(s) 282, 807, 1035, 1457, 1468, 1813
Sau3AI GATC 8 cut(s) 472, 909, 1393, 1531, 1536, 1610, 1622, 1906
Sau96I GGNCC 2 cut(s) 400, 2146
SchI GAGTC 1 cut(s) 1032
ScrFI CCNGG 2 cut(s) 1287, 1391
SduI GDGCHC 5 cut(s) 888, 1251, 1404, 1764, 2435
SfaNI GCATC 3 cut(s) 1000, 2226, 2312
Sfr274I CTCGAG 3 cut(s) 276, 1099, 1250
SfuI TTCGAA 1 cut(s) 524
SinI GGWCC 1 cut(s) 2146
SlaI CTCGAG 3 cut(s) 276, 1099, 1250
SmiMI CAYNNNNRTG 6 cut(s) 61, 500, 1767, 1769, 1936, 2271
SmlI CTYRAG 6 cut(s) 276, 314, 1099, 1250, 2317, 2447
SmoI CTYRAG 6 cut(s) 276, 314, 1099, 1250, 2317, 2447
SnaBI TACGTA 1 cut(s) 1842
SspMI CTAG 4 cut(s) 198, 383, 890, 1580
StyD4I CCNGG 2 cut(s) 1285, 1389
StyI CCWWGG 7 cut(s) 100, 622, 1029, 1081, 1149, 2058, 2344
TaaI ACNGT 4 cut(s) 1312, 1408, 1559, 1684
TaiI ACGT 2 cut(s) 1141, 1844
TaqI TCGA 6 cut(s) 277, 328, 524, 677, 1100, 1251
TatI WGTACW 4 cut(s) 7, 405, 513, 1559
TauI GCSGC 3 cut(s) 1037, 1470, 1815
TfiI GAWTC 3 cut(s) 679, 772, 1677
Tru1I TTAA 7 cut(s) 83, 156, 315, 2079, 2291, 2424, 2448
Tru9I TTAA 7 cut(s) 83, 156, 315, 2079, 2291, 2424, 2448
TscAI CASTG 4 cut(s) 43, 1687, 1894, 2440
TseFI GTSAC 3 cut(s) 699, 1139, 1301
TseI GCWGC 3 cut(s) 281, 806, 1456
Tsp45I GTSAC 3 cut(s) 699, 1139, 1301
TspGWI ACGGA 1 cut(s) 859
TspRI CASTG 4 cut(s) 43, 1687, 1894, 2440
Van91I CCANNNNNTGG 1 cut(s) 1087
Vha464I CTTAAG 2 cut(s) 314, 2447
VneI GTGCAC 1 cut(s) 2431
VpaK11BI GGWCC 1 cut(s) 2146
VspI ATTAAT 1 cut(s) 2424
XagI CCTNNNNNAGG 1 cut(s) 965
XapI RAATTY 3 cut(s) 2044, 2053, 2286
XceI RCATGY 2 cut(s) 268, 566
XcmI CCANNNNNNNNNTGG 1 cut(s) 1511
XhoI CTCGAG 3 cut(s) 276, 1099, 1250
XmnI GAANNNNTTC 1 cut(s) 393
XspI CTAG 4 cut(s) 198, 383, 890, 1580
Zsp2I ATGCAT 1 cut(s) 2327
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.