Rroxscaffold_4G00321030

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000549
Physical Location & Seq
Forward (+)
50087729 .. 50090295
2567 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00321030.1

Sequence Viewer

Length: 384 bp
ATGGCTACTATGTGGCCAAAGGTGGTTATAGGAGGCGGTGCGGAAAGGTCTAAACCGGTAGAAGATTTTAGCAGCCGGTTCGGTGCTTCGAGGCCGTTCGCTGCTTTTACGGCCGATTCTCTAGATTCCGCCGGTTCACCGGTCCTAAGGTCGGGGGCTACTACGTGGGCGGCGAGAGGCTCCGAACGGCCGGATGCGAGCGACAAAACCGTCGGCTGCGGTGACGACACCTTCAACACCTTCTTCTCCGAGACCGACAACGGCAAGCAAGTCCCACGCGCCATCTTAGTCGACCTCGAGCCCACCGTCATCGACGAGGTCCGCACGGAACCTCGCCGGCTCTTCCACCGGAGCGGCTCATCTCGGCAAGGAGGACACCGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04145 ko04210 ko04530 ko04540 ko05130 map04145 map04210 map04530 map04540 map05130
Pfam Domains
Protein Families
Tubulin Tubulin_C

Protein Analysis

127

Amino Acids

13.69

Weight (kDa)

6.73

Isoelectric Point (pI)

53.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tubulin PF00091 77 - 123 1.7e-06 Tubulin/FtsZ family, GTPase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000549)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04820 AT1G50010 AT4G14960 AT4G14960
fragaria_vesca FvH4_1g18660 FvH4_2g03140 FvH4_5g33560 FvH4_6g48940 FvH4_6g48940
malus_domestica MD02G1204800.v1.1 MD05G1106800.v1.1 MD08G1210600.v1.1 MD09G1049500.v1.1 MD10G1111600.v1.1 MD15G1398700.v1.1 MD17G1049700.v1.1
prunus_persica Prupe.1G542600_v2.0.a1 Prupe.3G269300_v2.0.a1 Prupe.6G212700_v2.0.a1 Prupe.8G150900_v2.0.a1
pyrus_communis pycom05g10370 pycom08g18150 pycom10g09640 pycom10g09660 pycom111g04050 pycom15g35470 pycom17g04460
rosa_chinensis RchiOBHm_Chr2g0109481 RchiOBHm_Chr2g0168751 RchiOBHm_Chr3g0453071 RchiOBHm_Chr3g0460741 RchiOBHm_Chr3g0467971 RchiOBHm_Chr4g0400901 RchiOBHm_Chr6g0248011 RchiOBHm_Chr7g0232381 RchiOBHm_Chr7g0238121
rosa_laevigata RLG00000001350 RLG00000009167 RLG00000015163 RLG00000017708 RLG00000017741 RLG00000021807 RLG00000023849 RLG00000026518
rosa_multiflora Rmu_sc0000332.1_g000021 Rmu_sc0000332.1_g000029 Rmu_sc0002416.1_g000007 Rmu_sc0002611.1_g000001 Rmu_sc0003113.1_g000016 Rmu_sc0004780.1_g000001 Rmu_sc0004805.1_g000035 Rmu_sc0010633.1_g000010 Rmu_sc0011069.1_g000003 Rmu_sc0016170.1_g000002 Rmu_sc0025757.1_g000003 Rmu_ssc0000484.1_g000003
rosa_roxburghii Rroxscaffold_2G00134200 Rroxscaffold_4G00321030 Rroxscaffold_7G00213500
rosa_rugosa Rorug01G0283100 Rorug02G0160500 Rorug02G0535300 Rorug04G0027000 Rorug04G0328100 Rorug05G0388700 Rorug05G0530200 Rorug06G0489200 Rorug07G0104900 Rorug07G0209400 Rorug07G0274500
rosa_samantha Rh2BG222800 Rh2BG618900 Rh2CG214400 Rh2CG587200 Rh6BG040200 Rh6CG206500 Rh6DG035600 Rh7BG401600 Rh7BG401700 Rh7DG418400
rosa_wichuraiana Rw2G050240 Rw6G003920 Rw7G035430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 209
AccBSI CCGCTC 1 cut(s) 354
AccI GTMKAC 1 cut(s) 291
AccII CGCG 1 cut(s) 279
AciI CCGC 8 cut(s) 36, 41, 129, 170, 219, 322, 354, 379
AcoI YGGCCR 3 cut(s) 14, 111, 188
AfiI CCNNNNNNNGG 1 cut(s) 151
AgeI ACCGGT 2 cut(s) 55, 139
AgsI TTSAA 1 cut(s) 235
Alw26I GTCTC 1 cut(s) 245
Ama87I CYCGRG 1 cut(s) 296
AoxI GGCC 4 cut(s) 14, 92, 111, 188
ApeKI GCWGC 3 cut(s) 72, 101, 216
AsiGI ACCGGT 2 cut(s) 55, 139
AspLEI GCGC 1 cut(s) 281
AspS9I GGNCC 2 cut(s) 142, 319
AsuHPI GGTGA 2 cut(s) 129, 233
AvaI CYCGRG 1 cut(s) 296
AvaII GGWCC 2 cut(s) 142, 319
AxyI CCTNAGG 1 cut(s) 146
BalI TGGCCA 1 cut(s) 16
BanII GRGCYC 1 cut(s) 303
BbvI GCAGC 3 cut(s) 84, 88, 203
BccI CCATC 1 cut(s) 290
BceAI ACGGC 4 cut(s) 79, 126, 203, 277
BcgI CGANNNNNNTGC 2 cut(s) 61, 95
BcoDI GTCTC 1 cut(s) 245
BfaI CTAG 1 cut(s) 122
BisI GCNGC 5 cut(s) 73, 102, 171, 217, 355
BlsI GCNGC 5 cut(s) 74, 103, 172, 218, 356
Bme18I GGWCC 2 cut(s) 142, 319
BmeT110I CYCGRG 1 cut(s) 296
BmgT120I GGNCC 2 cut(s) 142, 319
BmiI GGNNCC 2 cut(s) 181, 330
BmsI GCATC 1 cut(s) 184
BsaAI YACGTR 1 cut(s) 165
BsaI GGTCTC 1 cut(s) 245
BsaWI WCCGGW 3 cut(s) 55, 139, 348
Bsc4I CCNNNNNNNGG 1 cut(s) 151
Bse118I RCCGGY 5 cut(s) 55, 75, 131, 139, 336
Bse21I CCTNAGG 1 cut(s) 146
BseGI GGATG 1 cut(s) 199
BseLI CCNNNNNNNGG 1 cut(s) 151
BseX3I CGGCCG 2 cut(s) 111, 188
BseXI GCAGC 3 cut(s) 84, 88, 203
Bsh1236I CGCG 1 cut(s) 279
Bsh1285I CGRYCG 2 cut(s) 114, 191
BshFI GGCC 4 cut(s) 16, 94, 113, 190
BshTI ACCGGT 2 cut(s) 55, 139
BsiEI CGRYCG 2 cut(s) 114, 191
BsiHKCI CYCGRG 1 cut(s) 296
BsiSI CCGG 7 cut(s) 56, 76, 132, 140, 191, 337, 349
BslFI GGGAC 1 cut(s) 257
BslI CCNNNNNNNGG 1 cut(s) 151
BsmAI GTCTC 1 cut(s) 245
BsmFI GGGAC 1 cut(s) 257
BsnI GGCC 4 cut(s) 16, 94, 113, 190
Bso31I GGTCTC 1 cut(s) 245
BsoBI CYCGRG 1 cut(s) 296
Bsp1286I GDGCHC 1 cut(s) 303
BspACI CCGC 8 cut(s) 36, 41, 129, 170, 219, 322, 354, 379
BspANI GGCC 4 cut(s) 16, 94, 113, 190
BspFNI CGCG 1 cut(s) 279
BspLI GGNNCC 2 cut(s) 181, 330
BspQI GCTCTTC 1 cut(s) 347
BspTNI GGTCTC 1 cut(s) 245
BsrBI CCGCTC 1 cut(s) 354
BsrFI RCCGGY 5 cut(s) 55, 75, 131, 139, 336
BssAI RCCGGY 5 cut(s) 55, 75, 131, 139, 336
Bst4CI ACNGT 2 cut(s) 211, 307
Bst6I CTCTTC 1 cut(s) 347
BstBAI YACGTR 1 cut(s) 165
BstC8I GCNNGC 3 cut(s) 199, 266, 338
BstDEI CTNAG 2 cut(s) 146, 286
BstF5I GGATG 1 cut(s) 199
BstFNI CGCG 1 cut(s) 279
BstHHI GCGC 1 cut(s) 281
BstMAI GTCTC 1 cut(s) 245
BstMCI CGRYCG 2 cut(s) 114, 191
BstMWI GCNNNNNNNGC 1 cut(s) 110
BstUI CGCG 1 cut(s) 279
BstV1I GCAGC 3 cut(s) 84, 88, 203
BstZI CGGCCG 2 cut(s) 111, 188
Bsu36I CCTNAGG 1 cut(s) 146
BsuRI GGCC 4 cut(s) 16, 94, 113, 190
BtsCI GGATG 1 cut(s) 199
Cac8I GCNNGC 3 cut(s) 199, 266, 338
CfoI GCGC 1 cut(s) 281
Cfr10I RCCGGY 5 cut(s) 55, 75, 131, 139, 336
Cfr13I GGNCC 2 cut(s) 142, 319
CspAI ACCGGT 2 cut(s) 55, 139
DdeI CTNAG 2 cut(s) 146, 286
DrdI GACNNNNNNGTC 1 cut(s) 209
DseDI GACNNNNNNGTC 1 cut(s) 209
EaeI YGGCCR 3 cut(s) 14, 111, 188
EagI CGGCCG 2 cut(s) 111, 188
Eam1104I CTCTTC 1 cut(s) 347
EarI CTCTTC 1 cut(s) 347
EciI GGCGGA 1 cut(s) 118
EclXI CGGCCG 2 cut(s) 111, 188
Eco24I GRGCYC 1 cut(s) 303
Eco31I GGTCTC 1 cut(s) 245
Eco47I GGWCC 2 cut(s) 142, 319
Eco52I CGGCCG 2 cut(s) 111, 188
Eco81I CCTNAGG 1 cut(s) 146
Eco88I CYCGRG 1 cut(s) 296
EcoT38I GRGCYC 1 cut(s) 303
FaiI YATR 2 cut(s) 11, 29
FaqI GGGAC 1 cut(s) 257
FblI GTMKAC 1 cut(s) 291
Fnu4HI GCNGC 5 cut(s) 73, 102, 171, 217, 355
FokI GGATG 1 cut(s) 206
FriOI GRGCYC 1 cut(s) 303
Fsp4HI GCNGC 5 cut(s) 73, 102, 171, 217, 355
FspBI CTAG 1 cut(s) 122
GlaI GCGC 1 cut(s) 280
GluI GCNGC 5 cut(s) 73, 102, 171, 217, 355
HaeIII GGCC 4 cut(s) 16, 94, 113, 190
HapII CCGG 7 cut(s) 56, 76, 132, 140, 191, 337, 349
HhaI GCGC 1 cut(s) 281
Hin6I GCGC 1 cut(s) 279
HinP1I GCGC 1 cut(s) 279
HincII GTYRAC 1 cut(s) 292
HindII GTYRAC 1 cut(s) 292
HinfI GANTC 2 cut(s) 116, 125
HpaII CCGG 7 cut(s) 56, 76, 132, 140, 191, 337, 349
HphI GGTGA 2 cut(s) 129, 233
Hpy166II GTNNAC 2 cut(s) 137, 292
Hpy188I TCNGA 2 cut(s) 184, 250
Hpy188III TCNNGA 1 cut(s) 122
Hpy8I GTNNAC 2 cut(s) 137, 292
Hpy99I CGWCG 2 cut(s) 215, 317
HpyAV CCTTC 2 cut(s) 241, 250
HpyCH4III ACNGT 2 cut(s) 211, 307
HpyCH4IV ACGT 1 cut(s) 164
HpyF10VI GCNNNNNNNGC 1 cut(s) 110
HpyF3I CTNAG 2 cut(s) 146, 286
HpySE526I ACGT 1 cut(s) 164
HspAI GCGC 1 cut(s) 279
KroI GCCGGC 1 cut(s) 336
KroNI GCCGGC 1 cut(s) 338
LguI GCTCTTC 1 cut(s) 347
LmnI GCTCC 2 cut(s) 185, 351
LpnPI CCDG 7 cut(s) 69, 89, 145, 153, 204, 350, 362
Lsp1109I GCAGC 3 cut(s) 84, 88, 203
LweI GCATC 1 cut(s) 184
MaeI CTAG 1 cut(s) 122
MaeII ACGT 1 cut(s) 164
MaeIII GTNAC 1 cut(s) 221
MbiI CCGCTC 1 cut(s) 354
MboII GAAGA 3 cut(s) 74, 235, 334
MhlI GDGCHC 1 cut(s) 303
MlsI TGGCCA 1 cut(s) 16
MluNI TGGCCA 1 cut(s) 16
MnlI CCTC 7 cut(s) 26, 84, 170, 305, 310, 342, 365
Mox20I TGGCCA 1 cut(s) 16
MroNI GCCGGC 1 cut(s) 336
MscI TGGCCA 1 cut(s) 16
Msp20I TGGCCA 1 cut(s) 16
MspI CCGG 7 cut(s) 56, 76, 132, 140, 191, 337, 349
MvnI CGCG 1 cut(s) 279
MwoI GCNNNNNNNGC 1 cut(s) 110
NaeI GCCGGC 1 cut(s) 338
NgoMIV GCCGGC 1 cut(s) 336
NlaIV GGNNCC 2 cut(s) 181, 330
NmeAIII GCCGAG 1 cut(s) 343
NmuCI GTSAC 1 cut(s) 221
PaeR7I CTCGAG 1 cut(s) 296
PciSI GCTCTTC 1 cut(s) 347
PcsI WCGNNNNNNNCGW 2 cut(s) 170, 303
PdiI GCCGGC 1 cut(s) 338
PfeI GAWTC 2 cut(s) 116, 125
PflFI GACNNNGTC 1 cut(s) 317
PinAI ACCGGT 2 cut(s) 55, 139
PkrI GCNGC 5 cut(s) 74, 103, 172, 218, 356
Ppu21I YACGTR 1 cut(s) 165
PspN4I GGNNCC 2 cut(s) 181, 330
PspPI GGNCC 2 cut(s) 142, 319
PspXI VCTCGAGB 1 cut(s) 296
PsyI GACNNNGTC 1 cut(s) 317
SalI GTCGAC 1 cut(s) 290
SapI GCTCTTC 1 cut(s) 347
SatI GCNGC 5 cut(s) 73, 102, 171, 217, 355
Sau96I GGNCC 2 cut(s) 142, 319
SduI GDGCHC 1 cut(s) 303
SetI ASST 9 cut(s) 24, 50, 152, 167, 233, 242, 297, 321, 334
SfaNI GCATC 1 cut(s) 184
Sfr274I CTCGAG 1 cut(s) 296
SinI GGWCC 2 cut(s) 142, 319
SlaI CTCGAG 1 cut(s) 296
SmlI CTYRAG 1 cut(s) 296
SmoI CTYRAG 1 cut(s) 296
SsiI CCGC 8 cut(s) 36, 41, 129, 170, 219, 322, 354, 379
SspMI CTAG 1 cut(s) 122
TaaI ACNGT 2 cut(s) 211, 307
TaiI ACGT 1 cut(s) 167
TaqI TCGA 4 cut(s) 89, 291, 297, 312
TaqII GACCGA 1 cut(s) 269
TauI GCSGC 2 cut(s) 173, 357
TfiI GAWTC 2 cut(s) 116, 125
TseFI GTSAC 1 cut(s) 221
TseI GCWGC 3 cut(s) 72, 101, 216
Tsp45I GTSAC 1 cut(s) 221
TspGWI ACGGA 1 cut(s) 341
Tth111I GACNNNGTC 1 cut(s) 317
VpaK11BI GGWCC 2 cut(s) 142, 319
XbaI TCTAGA 1 cut(s) 121
XhoI CTCGAG 1 cut(s) 296
XmiI GTMKAC 1 cut(s) 291
XspI CTAG 1 cut(s) 122
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.