RchiOBHm_Chr3g0460741

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0000549
Physical Location & Seq
Reverse (-)
8730296 .. 8731062
767 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ42723

Sequence Viewer

Length: 612 bp
ATGGACGGGCTCGAGAAGCTACGTCGTCAGGAAAACGACATTCCGCCTGGCCAAATGCAGAGTGACAAGACAGTCGGGGGCGGAGACGACGCCTTCAACACTTTCTTCAATGAATCCGGAGCCGGATTGCACATCCCCCGCGCCCTCATCAGCGGCAAAGAGGACGTCGCTAATAACTTCACCAGAGGCCATTACACAATTGGGAAGGAGATTGTGGATCAGATCCGGAAGCTTGCGGACAACTGTACTGGGCTTCAATGGTTTCTAGTGTCCCATGCCGTCAGAGGTGGGACTGGATCTAGGCTTGGATCTCTACTCTTGGAGAGGCTCTTTGTGGACTATGGGAAGAAATCTAAGCTTGGATTCACTGTTTACCCATCTCCTCAGGTCTCAACCTCTGTTGTTGAGCCTTATAACAATGTTTTGTCCACTCGCTCGCTCTGGGAGCACACTAATGTGGCTGTGCTTCTGGACAATGAAGCCATCTATGATATCTGCCGCAGATTGCTTGACATTGAGCGACCTACGTATACCAATCTCAACAGGCTTGTTTCTCAGGTCATTTCCTCACTAACTGCTTCTCTGCTATTTGATCCAGCGTCAGATTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04145 ko04210 ko04530 ko04540 ko05130 map04145 map04210 map04530 map04540 map05130
Pfam Domains
Protein Families
Tubulin Tubulin_C

Protein Analysis

203

Amino Acids

22.48

Weight (kDa)

5.81

Isoelectric Point (pI)

36.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tubulin PF00091 46 - 166 4.3e-28 Tubulin/FtsZ family, GTPase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000549)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04820 AT1G50010 AT4G14960 AT4G14960
fragaria_vesca FvH4_1g18660 FvH4_2g03140 FvH4_5g33560 FvH4_6g48940 FvH4_6g48940
malus_domestica MD02G1204800.v1.1 MD05G1106800.v1.1 MD08G1210600.v1.1 MD09G1049500.v1.1 MD10G1111600.v1.1 MD15G1398700.v1.1 MD17G1049700.v1.1
prunus_persica Prupe.1G542600_v2.0.a1 Prupe.3G269300_v2.0.a1 Prupe.6G212700_v2.0.a1 Prupe.8G150900_v2.0.a1
pyrus_communis pycom05g10370 pycom08g18150 pycom10g09640 pycom10g09660 pycom111g04050 pycom15g35470 pycom17g04460
rosa_chinensis RchiOBHm_Chr2g0109481 RchiOBHm_Chr2g0168751 RchiOBHm_Chr3g0453071 RchiOBHm_Chr3g0460741 RchiOBHm_Chr3g0467971 RchiOBHm_Chr4g0400901 RchiOBHm_Chr6g0248011 RchiOBHm_Chr7g0232381 RchiOBHm_Chr7g0238121
rosa_laevigata RLG00000001350 RLG00000009167 RLG00000015163 RLG00000017708 RLG00000017741 RLG00000021807 RLG00000023849 RLG00000026518
rosa_multiflora Rmu_sc0000332.1_g000021 Rmu_sc0000332.1_g000029 Rmu_sc0002416.1_g000007 Rmu_sc0002611.1_g000001 Rmu_sc0003113.1_g000016 Rmu_sc0004780.1_g000001 Rmu_sc0004805.1_g000035 Rmu_sc0010633.1_g000010 Rmu_sc0011069.1_g000003 Rmu_sc0016170.1_g000002 Rmu_sc0025757.1_g000003 Rmu_ssc0000484.1_g000003
rosa_roxburghii Rroxscaffold_2G00134200 Rroxscaffold_4G00321030 Rroxscaffold_7G00213500
rosa_rugosa Rorug01G0283100 Rorug02G0160500 Rorug02G0535300 Rorug04G0027000 Rorug04G0328100 Rorug05G0388700 Rorug05G0530200 Rorug06G0489200 Rorug07G0104900 Rorug07G0209400 Rorug07G0274500
rosa_samantha Rh2BG222800 Rh2BG618900 Rh2CG214400 Rh2CG587200 Rh6BG040200 Rh6CG206500 Rh6DG035600 Rh7BG401600 Rh7BG401700 Rh7DG418400
rosa_wichuraiana Rw2G050240 Rw6G003920 Rw7G035430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 414
AasI GACNNNNNNGTC 1 cut(s) 71
AatII GACGTC 1 cut(s) 168
AccI GTMKAC 1 cut(s) 530
AccII CGCG 1 cut(s) 141
AccIII TCCGGA 2 cut(s) 116, 225
AciI CCGC 6 cut(s) 44, 81, 139, 153, 236, 499
AclWI GGATC 5 cut(s) 217, 225, 304, 316, 587
AcoI YGGCCR 1 cut(s) 49
AcyI GRCGYC 2 cut(s) 90, 165
AfaI GTAC 1 cut(s) 247
AgsI TTSAA 3 cut(s) 97, 109, 257
AjnI CCWGG 1 cut(s) 46
AleI CACNNNNGTG 1 cut(s) 455
AluBI AGCT 3 cut(s) 19, 232, 358
AluI AGCT 3 cut(s) 19, 232, 358
Alw21I GWGCWC 1 cut(s) 450
Alw26I GTCTC 2 cut(s) 78, 394
AlwI GGATC 5 cut(s) 217, 225, 304, 316, 587
Ama87I CYCGRG 1 cut(s) 11
Aor13HI TCCGGA 2 cut(s) 116, 225
AoxI GGCC 2 cut(s) 49, 187
ArsI GACNNNNNNTTYG 2 cut(s) 150, 182
AspLEI GCGC 1 cut(s) 143
AsuHPI GGTGA 1 cut(s) 172
AvaI CYCGRG 1 cut(s) 11
AxyI CCTNAGG 1 cut(s) 384
BalI TGGCCA 1 cut(s) 51
BanII GRGCYC 1 cut(s) 12
Bbv12I GWGCWC 1 cut(s) 450
BccI CCATC 2 cut(s) 385, 491
BceAI ACGGC 1 cut(s) 263
BciT130I CCWGG 1 cut(s) 48
BcoDI GTCTC 2 cut(s) 78, 394
BfaI CTAG 2 cut(s) 266, 300
BisI GCNGC 2 cut(s) 154, 499
BlsI GCNGC 2 cut(s) 155, 500
Bme1390I CCNGG 1 cut(s) 48
BmeT110I CYCGRG 1 cut(s) 11
BmiI GGNNCC 1 cut(s) 121
BmrFI CCNGG 1 cut(s) 48
BmrI ACTGGG 1 cut(s) 258
BmuI ACTGGG 1 cut(s) 258
BsaAI YACGTR 1 cut(s) 528
BsaHI GRCGYC 2 cut(s) 90, 165
BsaI GGTCTC 1 cut(s) 394
BsaWI WCCGGW 2 cut(s) 116, 225
Bse1I ACTGG 2 cut(s) 253, 298
Bse21I CCTNAGG 1 cut(s) 384
BseAI TCCGGA 2 cut(s) 116, 225
BseBI CCWGG 1 cut(s) 48
BseGI GGATG 1 cut(s) 132
BseMII CTCAG 2 cut(s) 398, 569
BseNI ACTGG 2 cut(s) 253, 298
BseRI GAGGAG 1 cut(s) 372
Bsh1236I CGCG 1 cut(s) 141
BshFI GGCC 2 cut(s) 51, 189
BsiHKAI GWGCWC 1 cut(s) 450
BsiHKCI CYCGRG 1 cut(s) 11
BsiSI CCGG 3 cut(s) 117, 123, 226
BslFI GGGAC 2 cut(s) 256, 304
BsmAI GTCTC 2 cut(s) 78, 394
BsmBI CGTCTC 1 cut(s) 78
BsmFI GGGAC 2 cut(s) 256, 304
BsnI GGCC 2 cut(s) 51, 189
Bso31I GGTCTC 1 cut(s) 394
BsoBI CYCGRG 1 cut(s) 11
Bsp1286I GDGCHC 2 cut(s) 12, 450
Bsp13I TCCGGA 2 cut(s) 116, 225
Bsp143I GATC 5 cut(s) 217, 222, 296, 308, 592
BspACI CCGC 6 cut(s) 44, 81, 139, 153, 236, 499
BspANI GGCC 2 cut(s) 51, 189
BspCNI CTCAG 2 cut(s) 397, 568
BspEI TCCGGA 2 cut(s) 116, 225
BspFNI CGCG 1 cut(s) 141
BspLI GGNNCC 1 cut(s) 121
BspPI GGATC 5 cut(s) 217, 225, 304, 316, 587
BspTNI GGTCTC 1 cut(s) 394
BsrI ACTGG 2 cut(s) 253, 298
BssMI GATC 5 cut(s) 217, 222, 296, 308, 592
BssNAI GTATAC 1 cut(s) 531
BssNI GRCGYC 2 cut(s) 90, 165
Bst1107I GTATAC 1 cut(s) 531
Bst2UI CCWGG 1 cut(s) 48
Bst4CI ACNGT 3 cut(s) 73, 245, 370
BstACI GRCGYC 2 cut(s) 90, 165
BstBAI YACGTR 1 cut(s) 528
BstC8I GCNNGC 2 cut(s) 234, 437
BstDEI CTNAG 3 cut(s) 354, 384, 555
BstF5I GGATG 1 cut(s) 132
BstFNI CGCG 1 cut(s) 141
BstHHI GCGC 1 cut(s) 143
BstKTI GATC 5 cut(s) 220, 225, 299, 311, 595
BstMAI GTCTC 2 cut(s) 78, 394
BstMBI GATC 5 cut(s) 217, 222, 296, 308, 592
BstMWI GCNNNNNNNGC 2 cut(s) 16, 445
BstNI CCWGG 1 cut(s) 48
BstSCI CCNGG 1 cut(s) 46
BstSNI TACGTA 1 cut(s) 528
BstUI CGCG 1 cut(s) 141
BstX2I RGATCY 3 cut(s) 222, 296, 308
BstYI RGATCY 3 cut(s) 222, 296, 308
BstZ17I GTATAC 1 cut(s) 531
Bsu36I CCTNAGG 1 cut(s) 384
BsuRI GGCC 2 cut(s) 51, 189
BtsCI GGATG 1 cut(s) 132
BtsIMutI CAGTG 1 cut(s) 366
Cac8I GCNNGC 2 cut(s) 234, 437
CfoI GCGC 1 cut(s) 143
CseI GACGC 2 cut(s) 98, 588
Csp6I GTAC 1 cut(s) 246
CviAII CATG 1 cut(s) 275
CviQI GTAC 1 cut(s) 246
DdeI CTNAG 3 cut(s) 354, 384, 555
DpnI GATC 5 cut(s) 219, 224, 298, 310, 594
DpnII GATC 5 cut(s) 217, 222, 296, 308, 592
DrdI GACNNNNNNGTC 1 cut(s) 71
DseDI GACNNNNNNGTC 1 cut(s) 71
EaeI YGGCCR 1 cut(s) 49
EciI GGCGGA 2 cut(s) 33, 96
Eco105I TACGTA 1 cut(s) 528
Eco24I GRGCYC 1 cut(s) 12
Eco31I GGTCTC 1 cut(s) 394
Eco32I GATATC 1 cut(s) 493
Eco81I CCTNAGG 1 cut(s) 384
Eco88I CYCGRG 1 cut(s) 11
EcoRII CCWGG 1 cut(s) 46
EcoRV GATATC 1 cut(s) 493
EcoT38I GRGCYC 1 cut(s) 12
Esp3I CGTCTC 1 cut(s) 78
FaeI CATG 1 cut(s) 278
FaiI YATR 5 cut(s) 276, 342, 414, 489, 531
FaqI GGGAC 2 cut(s) 256, 304
FatI CATG 1 cut(s) 274
FauI CCCGC 1 cut(s) 146
FblI GTMKAC 1 cut(s) 530
Fnu4HI GCNGC 2 cut(s) 154, 499
FokI GGATG 1 cut(s) 119
FriOI GRGCYC 1 cut(s) 12
Fsp4HI GCNGC 2 cut(s) 154, 499
FspBI CTAG 2 cut(s) 266, 300
GlaI GCGC 1 cut(s) 142
GluI GCNGC 2 cut(s) 154, 499
HaeIII GGCC 2 cut(s) 51, 189
HapII CCGG 3 cut(s) 117, 123, 226
HgaI GACGC 2 cut(s) 98, 588
HhaI GCGC 1 cut(s) 143
Hin1I GRCGYC 2 cut(s) 90, 165
Hin1II CATG 1 cut(s) 278
Hin6I GCGC 1 cut(s) 141
HinP1I GCGC 1 cut(s) 141
HindIII AAGCTT 2 cut(s) 230, 356
HinfI GANTC 2 cut(s) 113, 363
HpaII CCGG 3 cut(s) 117, 123, 226
HphI GGTGA 1 cut(s) 172
Hpy166II GTNNAC 4 cut(s) 337, 373, 429, 531
Hpy188I TCNGA 3 cut(s) 222, 284, 604
Hpy188III TCNNGA 5 cut(s) 13, 29, 117, 226, 470
Hpy8I GTNNAC 4 cut(s) 337, 373, 429, 531
Hpy99I CGWCG 3 cut(s) 27, 92, 170
HpyAV CCTTC 2 cut(s) 103, 199
HpyCH4III ACNGT 3 cut(s) 73, 245, 370
HpyCH4IV ACGT 3 cut(s) 22, 165, 527
HpyCH4V TGCA 2 cut(s) 58, 130
HpyF10VI GCNNNNNNNGC 2 cut(s) 16, 445
HpyF3I CTNAG 3 cut(s) 354, 384, 555
HpySE526I ACGT 3 cut(s) 22, 165, 527
Hsp92I GRCGYC 2 cut(s) 90, 165
Hsp92II CATG 1 cut(s) 278
HspAI GCGC 1 cut(s) 141
Kpn2I TCCGGA 2 cut(s) 116, 225
Kzo9I GATC 5 cut(s) 217, 222, 296, 308, 592
LmnI GCTCC 2 cut(s) 119, 445
MaeI CTAG 2 cut(s) 266, 300
MaeII ACGT 3 cut(s) 22, 165, 527
MaeIII GTNAC 1 cut(s) 62
MalI GATC 5 cut(s) 219, 224, 298, 310, 594
MboI GATC 5 cut(s) 217, 222, 296, 308, 592
MboII GAAGA 2 cut(s) 97, 358
MfeI CAATTG 1 cut(s) 198
MflI RGATCY 3 cut(s) 222, 296, 308
MhlI GDGCHC 2 cut(s) 12, 450
MlsI TGGCCA 1 cut(s) 51
MluCI AATT 1 cut(s) 198
MluNI TGGCCA 1 cut(s) 51
MnlI CCTC 8 cut(s) 154, 155, 179, 278, 318, 393, 406, 577
Mox20I TGGCCA 1 cut(s) 51
MroI TCCGGA 2 cut(s) 116, 225
MscI TGGCCA 1 cut(s) 51
MslI CAYNNNNRTG 2 cut(s) 453, 455
Msp20I TGGCCA 1 cut(s) 51
MspA1I CMGCKG 1 cut(s) 153
MspI CCGG 3 cut(s) 117, 123, 226
MspR9I CCNGG 1 cut(s) 48
MunI CAATTG 1 cut(s) 198
MvaI CCWGG 1 cut(s) 48
MvnI CGCG 1 cut(s) 141
MwoI GCNNNNNNNGC 2 cut(s) 16, 445
NdeII GATC 5 cut(s) 217, 222, 296, 308, 592
NlaIII CATG 1 cut(s) 278
NlaIV GGNNCC 1 cut(s) 121
NmuCI GTSAC 1 cut(s) 62
OliI CACNNNNGTG 1 cut(s) 455
PaeR7I CTCGAG 1 cut(s) 11
PfeI GAWTC 2 cut(s) 113, 363
PkrI GCNGC 2 cut(s) 155, 500
Ppu21I YACGTR 1 cut(s) 528
PsiI TTATAA 1 cut(s) 414
Psp6I CCWGG 1 cut(s) 46
PspGI CCWGG 1 cut(s) 46
PspN4I GGNNCC 1 cut(s) 121
PsuI RGATCY 3 cut(s) 222, 296, 308
RsaI GTAC 1 cut(s) 247
RsaNI GTAC 1 cut(s) 246
RseI CAYNNNNRTG 2 cut(s) 453, 455
SatI GCNGC 2 cut(s) 154, 499
Sau3AI GATC 5 cut(s) 217, 222, 296, 308, 592
ScrFI CCNGG 1 cut(s) 48
SduI GDGCHC 2 cut(s) 12, 450
Sfr274I CTCGAG 1 cut(s) 11
SlaI CTCGAG 1 cut(s) 11
SmiMI CAYNNNNRTG 2 cut(s) 453, 455
SmlI CTYRAG 1 cut(s) 11
SmoI CTYRAG 1 cut(s) 11
SnaBI TACGTA 1 cut(s) 528
Sse9I AATT 1 cut(s) 198
SsiI CCGC 6 cut(s) 44, 81, 139, 153, 236, 499
SspMI CTAG 2 cut(s) 266, 300
StyD4I CCNGG 1 cut(s) 46
TaaI ACNGT 3 cut(s) 73, 245, 370
TaiI ACGT 3 cut(s) 25, 168, 530
TaqI TCGA 1 cut(s) 12
TasI AATT 1 cut(s) 198
TatI WGTACW 1 cut(s) 245
TauI GCSGC 2 cut(s) 156, 501
TfiI GAWTC 2 cut(s) 113, 363
TscAI CASTG 1 cut(s) 373
TseFI GTSAC 1 cut(s) 62
Tsp45I GTSAC 1 cut(s) 62
TspDTI ATGAA 2 cut(s) 126, 492
TspRI CASTG 1 cut(s) 373
XcmI CCANNNNNNNNNTGG 1 cut(s) 197
XhoI CTCGAG 1 cut(s) 11
XmiI GTMKAC 1 cut(s) 530
XspI CTAG 2 cut(s) 266, 300
ZraI GACGTC 1 cut(s) 166
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.