Rmu_sc0004805.1_g000035

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004805.1
OG0000549
Physical Location & Seq
Forward (+)
193222 .. 193737
516 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004805.1_g000035.1.cds

Sequence Viewer

Length: 516 bp
atgccgagcgacaagaccgttggctgcgacgacgacaccttcaacaccttcttctccaagaccggcaccagcaagcacgtcccacgtgccatcttcttctacctcgagcccaccgtcatcgacgaggtccgcaccggaacctactgccagctcttccacccggaacaactcatctccggcaaggaggacaccgctaacaacttcgcatgtggtcactacaccatcggcaaagagatcgtcaatctctgcctggatcagatccggaagatcgctgataactgcaccgggctccagggatccctggtcttcaatgccatctgtggcgatactgggtcgggtctcgggtcgcttctactggagaggctttttgtggactatgggaaaaagtccaagctcggattcactgtgtatccttctcctcaggtttctacgtctattgtggagccctataacagtgtgttctccactcattctctacttgagcacaccgatgcgtctgtgttgctcaattactga

Protein Analysis

171

Amino Acids

18.81

Weight (kDa)

5.24

Isoelectric Point (pI)

24.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000549)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04820 AT1G50010 AT4G14960 AT4G14960
fragaria_vesca FvH4_1g18660 FvH4_2g03140 FvH4_5g33560 FvH4_6g48940 FvH4_6g48940
malus_domestica MD02G1204800.v1.1 MD05G1106800.v1.1 MD08G1210600.v1.1 MD09G1049500.v1.1 MD10G1111600.v1.1 MD15G1398700.v1.1 MD17G1049700.v1.1
prunus_persica Prupe.1G542600_v2.0.a1 Prupe.3G269300_v2.0.a1 Prupe.6G212700_v2.0.a1 Prupe.8G150900_v2.0.a1
pyrus_communis pycom05g10370 pycom08g18150 pycom10g09640 pycom10g09660 pycom111g04050 pycom15g35470 pycom17g04460
rosa_chinensis RchiOBHm_Chr2g0109481 RchiOBHm_Chr2g0168751 RchiOBHm_Chr3g0453071 RchiOBHm_Chr3g0460741 RchiOBHm_Chr3g0467971 RchiOBHm_Chr4g0400901 RchiOBHm_Chr6g0248011 RchiOBHm_Chr7g0232381 RchiOBHm_Chr7g0238121
rosa_laevigata RLG00000001350 RLG00000009167 RLG00000015163 RLG00000017708 RLG00000017741 RLG00000021807 RLG00000023849 RLG00000026518
rosa_multiflora Rmu_sc0000332.1_g000021 Rmu_sc0000332.1_g000029 Rmu_sc0002416.1_g000007 Rmu_sc0002611.1_g000001 Rmu_sc0003113.1_g000016 Rmu_sc0004780.1_g000001 Rmu_sc0004805.1_g000035 Rmu_sc0010633.1_g000010 Rmu_sc0011069.1_g000003 Rmu_sc0016170.1_g000002 Rmu_sc0025757.1_g000003 Rmu_ssc0000484.1_g000003
rosa_roxburghii Rroxscaffold_2G00134200 Rroxscaffold_4G00321030 Rroxscaffold_7G00213500
rosa_rugosa Rorug01G0283100 Rorug02G0160500 Rorug02G0535300 Rorug04G0027000 Rorug04G0328100 Rorug05G0388700 Rorug05G0530200 Rorug06G0489200 Rorug07G0104900 Rorug07G0209400 Rorug07G0274500
rosa_samantha Rh2BG222800 Rh2BG618900 Rh2CG214400 Rh2CG587200 Rh6BG040200 Rh6CG206500 Rh6DG035600 Rh7BG401600 Rh7BG401700 Rh7DG418400
rosa_wichuraiana Rw2G050240 Rw6G003920 Rw7G035430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 65
AccIII TCCGGA 1 cut(s) 261
AciI CCGC 2 cut(s) 130, 192
AclWI GGATC 4 cut(s) 253, 261, 291, 304
AcvI CACGTG 1 cut(s) 86
AgsI TTSAA 2 cut(s) 43, 310
AjiI CACGTC 1 cut(s) 79
AjnI CCWGG 3 cut(s) 249, 291, 300
AjuI GAANNNNNNNTTGG 2 cut(s) 383, 415
AluBI AGCT 2 cut(s) 151, 394
AluI AGCT 2 cut(s) 151, 394
Alw21I GWGCWC 1 cut(s) 486
Alw26I GTCTC 1 cut(s) 344
AlwI GGATC 4 cut(s) 253, 261, 291, 304
Ama87I CYCGRG 2 cut(s) 104, 341
Aor13HI TCCGGA 1 cut(s) 261
ApeKI GCWGC 1 cut(s) 24
ArsI GACNNNNNNTTYG 2 cut(s) 222, 254
AspS9I GGNCC 1 cut(s) 127
AsuC2I CCSGG 2 cut(s) 161, 286
AvaI CYCGRG 2 cut(s) 104, 341
AvaII GGWCC 1 cut(s) 127
AxyI CCTNAGG 1 cut(s) 420
BamHI GGATCC 1 cut(s) 296
BanI GGYRCC 1 cut(s) 65
BanII GRGCYC 3 cut(s) 111, 291, 447
BbrPI CACGTG 1 cut(s) 86
BbsI GAAGAC 1 cut(s) 298
Bbv12I GWGCWC 1 cut(s) 486
BbvI GCAGC 1 cut(s) 11
BccI CCATC 3 cut(s) 98, 230, 323
BcgI CGANNNNNNTGC 2 cut(s) 217, 251
BciT130I CCWGG 3 cut(s) 251, 293, 302
BciVI GTATCC 1 cut(s) 420
BcnI CCSGG 2 cut(s) 161, 286
BcoDI GTCTC 1 cut(s) 344
BfuI GTATCC 1 cut(s) 420
BisI GCNGC 1 cut(s) 25
BlsI GCNGC 1 cut(s) 26
Bme1390I CCNGG 5 cut(s) 161, 251, 286, 293, 302
Bme18I GGWCC 1 cut(s) 127
BmeT110I CYCGRG 2 cut(s) 104, 341
BmgBI CACGTC 1 cut(s) 79
BmgT120I GGNCC 1 cut(s) 127
BmiI GGNNCC 5 cut(s) 67, 139, 290, 298, 444
BmrFI CCNGG 5 cut(s) 161, 251, 286, 293, 302
BmrI ACTGGG 1 cut(s) 339
BmsI GCATC 1 cut(s) 481
BmuI ACTGGG 1 cut(s) 339
BpiI GAAGAC 1 cut(s) 298
BpmI CTGGAG 2 cut(s) 275, 377
BpuEI CTTGAG 1 cut(s) 500
BpuMI CCSGG 2 cut(s) 161, 286
BsaAI YACGTR 1 cut(s) 86
BsaI GGTCTC 1 cut(s) 344
BsaJI CCNNGG 2 cut(s) 292, 300
BsaWI WCCGGW 2 cut(s) 134, 261
Bse118I RCCGGY 1 cut(s) 62
Bse1I ACTGG 2 cut(s) 334, 360
Bse21I CCTNAGG 1 cut(s) 420
BseAI TCCGGA 1 cut(s) 261
BseBI CCWGG 3 cut(s) 251, 293, 302
BseDI CCNNGG 2 cut(s) 292, 300
BseMII CTCAG 1 cut(s) 434
BseNI ACTGG 2 cut(s) 334, 360
BseRI GAGGAG 1 cut(s) 408
BseXI GCAGC 1 cut(s) 11
BsgI GTGCAG 1 cut(s) 265
BshNI GGYRCC 1 cut(s) 65
BsiHKAI GWGCWC 1 cut(s) 486
BsiHKCI CYCGRG 2 cut(s) 104, 341
BsiSI CCGG 6 cut(s) 63, 135, 161, 177, 262, 285
BslFI GGGAC 1 cut(s) 65
BsmAI GTCTC 1 cut(s) 344
BsmFI GGGAC 1 cut(s) 65
Bso31I GGTCTC 1 cut(s) 344
BsoBI CYCGRG 2 cut(s) 104, 341
Bsp1286I GDGCHC 4 cut(s) 111, 291, 447, 486
Bsp13I TCCGGA 1 cut(s) 261
Bsp143I GATC 5 cut(s) 234, 253, 258, 267, 296
BspACI CCGC 2 cut(s) 130, 192
BspCNI CTCAG 1 cut(s) 433
BspEI TCCGGA 1 cut(s) 261
BspLI GGNNCC 5 cut(s) 67, 139, 290, 298, 444
BspPI GGATC 4 cut(s) 253, 261, 291, 304
BspQI GCTCTTC 1 cut(s) 158
BspT107I GGYRCC 1 cut(s) 65
BspTNI GGTCTC 1 cut(s) 344
BsrFI RCCGGY 1 cut(s) 62
BsrI ACTGG 2 cut(s) 334, 360
BssAI RCCGGY 1 cut(s) 62
BssECI CCNNGG 2 cut(s) 292, 300
BssMI GATC 5 cut(s) 234, 253, 258, 267, 296
Bst2UI CCWGG 3 cut(s) 251, 293, 302
Bst4CI ACNGT 4 cut(s) 19, 115, 406, 455
Bst6I CTCTTC 1 cut(s) 158
BstBAI YACGTR 1 cut(s) 86
BstC8I GCNNGC 2 cut(s) 74, 149
BstDEI CTNAG 1 cut(s) 420
BstKTI GATC 5 cut(s) 237, 256, 261, 270, 299
BstMAI GTCTC 1 cut(s) 344
BstMBI GATC 5 cut(s) 234, 253, 258, 267, 296
BstNI CCWGG 3 cut(s) 251, 293, 302
BstNSI RCATGY 1 cut(s) 210
BstSCI CCNGG 5 cut(s) 159, 249, 284, 291, 300
BstV1I GCAGC 1 cut(s) 11
BstV2I GAAGAC 1 cut(s) 298
BstX2I RGATCY 2 cut(s) 258, 296
BstYI RGATCY 2 cut(s) 258, 296
Bsu36I CCTNAGG 1 cut(s) 420
BsuI GTATCC 1 cut(s) 420
BtrI CACGTC 1 cut(s) 79
BtsIMutI CAGTG 2 cut(s) 402, 460
Cac8I GCNNGC 2 cut(s) 74, 149
Cfr10I RCCGGY 1 cut(s) 62
Cfr13I GGNCC 1 cut(s) 127
CseI GACGC 1 cut(s) 483
CviAII CATG 1 cut(s) 207
CviJI RGCY 7 cut(s) 24, 109, 151, 289, 364, 394, 445
CviKI_1 RGCY 7 cut(s) 24, 109, 151, 289, 364, 394, 445
DdeI CTNAG 1 cut(s) 420
DpnI GATC 5 cut(s) 236, 255, 260, 269, 298
DpnII GATC 5 cut(s) 234, 253, 258, 267, 296
Eam1104I CTCTTC 1 cut(s) 158
EarI CTCTTC 1 cut(s) 158
Eco24I GRGCYC 3 cut(s) 111, 291, 447
Eco31I GGTCTC 1 cut(s) 344
Eco47I GGWCC 1 cut(s) 127
Eco72I CACGTG 1 cut(s) 86
Eco81I CCTNAGG 1 cut(s) 420
Eco88I CYCGRG 2 cut(s) 104, 341
EcoRII CCWGG 3 cut(s) 249, 291, 300
EcoT38I GRGCYC 3 cut(s) 111, 291, 447
FaeI CATG 1 cut(s) 210
FaiI YATR 3 cut(s) 208, 378, 450
FaqI GGGAC 1 cut(s) 65
FatI CATG 1 cut(s) 206
Fnu4HI GCNGC 1 cut(s) 25
FriOI GRGCYC 3 cut(s) 111, 291, 447
Fsp4HI GCNGC 1 cut(s) 25
GluI GCNGC 1 cut(s) 25
GsuI CTGGAG 2 cut(s) 275, 377
HapII CCGG 6 cut(s) 63, 135, 161, 177, 262, 285
HgaI GACGC 1 cut(s) 483
Hin1II CATG 1 cut(s) 210
HinfI GANTC 1 cut(s) 399
HpaII CCGG 6 cut(s) 63, 135, 161, 177, 262, 285
Hpy166II GTNNAC 1 cut(s) 373
Hpy188I TCNGA 2 cut(s) 258, 398
Hpy188III TCNNGA 1 cut(s) 262
Hpy8I GTNNAC 1 cut(s) 373
Hpy99I CGWCG 3 cut(s) 32, 35, 125
HpyAV CCTTC 3 cut(s) 49, 58, 423
HpyCH4III ACNGT 4 cut(s) 19, 115, 406, 455
HpyCH4IV ACGT 3 cut(s) 78, 85, 431
HpyCH4V TGCA 1 cut(s) 282
HpyF3I CTNAG 1 cut(s) 420
HpySE526I ACGT 3 cut(s) 78, 85, 431
Hsp92II CATG 1 cut(s) 210
Kpn2I TCCGGA 1 cut(s) 261
Kzo9I GATC 5 cut(s) 234, 253, 258, 267, 296
LguI GCTCTTC 1 cut(s) 158
LmnI GCTCC 2 cut(s) 294, 442
Lsp1109I GCAGC 1 cut(s) 11
LweI GCATC 1 cut(s) 481
MaeII ACGT 3 cut(s) 78, 85, 431
MaeIII GTNAC 1 cut(s) 212
MalI GATC 5 cut(s) 236, 255, 260, 269, 298
MboI GATC 5 cut(s) 234, 253, 258, 267, 296
MboII GAAGA 6 cut(s) 43, 85, 88, 145, 277, 298
MflI RGATCY 2 cut(s) 258, 296
MhlI GDGCHC 4 cut(s) 111, 291, 447, 486
MluCI AATT 1 cut(s) 508
MnlI CCTC 5 cut(s) 113, 118, 178, 354, 429
MroI TCCGGA 1 cut(s) 261
MslI CAYNNNNRTG 1 cut(s) 489
MspI CCGG 6 cut(s) 63, 135, 161, 177, 262, 285
MspR9I CCNGG 5 cut(s) 161, 251, 286, 293, 302
MvaI CCWGG 3 cut(s) 251, 293, 302
NciI CCSGG 2 cut(s) 161, 286
NdeII GATC 5 cut(s) 234, 253, 258, 267, 296
NlaIII CATG 1 cut(s) 210
NlaIV GGNNCC 5 cut(s) 67, 139, 290, 298, 444
NmeAIII GCCGAG 1 cut(s) 30
NmuCI GTSAC 1 cut(s) 212
NspI RCATGY 1 cut(s) 210
PaeR7I CTCGAG 1 cut(s) 104
PciSI GCTCTTC 1 cut(s) 158
PcsI WCGNNNNNNNCGW 1 cut(s) 111
PfeI GAWTC 1 cut(s) 399
PflFI GACNNNGTC 1 cut(s) 125
PkrI GCNGC 1 cut(s) 26
PmaCI CACGTG 1 cut(s) 86
PmlI CACGTG 1 cut(s) 86
Ppu21I YACGTR 1 cut(s) 86
Psp6I CCWGG 3 cut(s) 249, 291, 300
PspCI CACGTG 1 cut(s) 86
PspGI CCWGG 3 cut(s) 249, 291, 300
PspN4I GGNNCC 5 cut(s) 67, 139, 290, 298, 444
PspPI GGNCC 1 cut(s) 127
PspXI VCTCGAGB 1 cut(s) 104
PsuI RGATCY 2 cut(s) 258, 296
PsyI GACNNNGTC 1 cut(s) 125
RseI CAYNNNNRTG 1 cut(s) 489
SapI GCTCTTC 1 cut(s) 158
SatI GCNGC 1 cut(s) 25
Sau3AI GATC 5 cut(s) 234, 253, 258, 267, 296
Sau96I GGNCC 1 cut(s) 127
ScrFI CCNGG 5 cut(s) 161, 251, 286, 293, 302
SduI GDGCHC 4 cut(s) 111, 291, 447, 486
SfaNI GCATC 1 cut(s) 481
Sfr274I CTCGAG 1 cut(s) 104
SinI GGWCC 1 cut(s) 127
SlaI CTCGAG 1 cut(s) 104
SmiMI CAYNNNNRTG 1 cut(s) 489
SmlI CTYRAG 2 cut(s) 104, 479
SmoI CTYRAG 2 cut(s) 104, 479
Sse9I AATT 1 cut(s) 508
SsiI CCGC 2 cut(s) 130, 192
StyD4I CCNGG 5 cut(s) 159, 249, 284, 291, 300
TaaI ACNGT 4 cut(s) 19, 115, 406, 455
TaiI ACGT 3 cut(s) 81, 88, 434
TaqI TCGA 2 cut(s) 105, 120
TasI AATT 1 cut(s) 508
TfiI GAWTC 1 cut(s) 399
TscAI CASTG 2 cut(s) 409, 460
TseFI GTSAC 1 cut(s) 212
TseI GCWGC 1 cut(s) 24
Tsp45I GTSAC 1 cut(s) 212
TspRI CASTG 2 cut(s) 409, 460
Tth111I GACNNNGTC 1 cut(s) 125
VpaK11BI GGWCC 1 cut(s) 127
XceI RCATGY 1 cut(s) 210
XhoI CTCGAG 1 cut(s) 104
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.